CN220246089U

CN220246089U - Gene sequencing kit

Info

Publication number: CN220246089U
Application number: CN202322138505.1U
Authority: CN
Inventors: 易军林; 尚文宾; 冯秋杰; 侯平
Original assignee: Mygenostics Chongqing Gene Technology Co ltd
Current assignee: Mygenostics Chongqing Gene Technology Co ltd
Priority date: 2023-08-09
Filing date: 2023-08-09
Publication date: 2023-12-26
Anticipated expiration: 2033-08-09

Abstract

The utility model discloses a gene sequencing kit, which comprises: the bottom box body is provided with at least two storage tanks, the storage tanks are used for storing reaction substances, one storage tank is a magnetic bead separation tank, an inclined guide wall is formed at the bottom of the magnetic bead separation tank, one side wall of the magnetic bead separation tank is an adsorption wall, the inclined guide wall is used for guiding magnetic beads to the adsorption wall, an adsorption execution block is installed outside the bottom box body and fixed to the output end of the pushing mechanism, the pushing mechanism is used for driving the adsorption execution block to be far away from or close to the magnetic bead separation tank, and the adsorption execution block is located on one side of the adsorption wall, which is far away from the inclined guide wall. The gene sequencing kit solves the problem that magnetic beads cannot be automatically separated from a solution in the prior art.

Description

Gene sequencing kit

Technical Field

The utility model relates to equipment for gene sequencing, in particular to a gene sequencing kit.

Background

In the gene sequencing process, the magnetic beads are required to be used for adsorbing DNA fragments in the reaction process, the magnetic beads are mixed in the reaction solution, and the magnetic beads are required to be separated from the solution in the elution process, so how to realize the rapid and automatic separation of the magnetic beads from the solution is a topic in the discussion of the application.

The Chinese patent discloses a gene detection kit based on a nano magnetic bead method, which comprises a box body, a sealing cover, an observation window, a label, a comparison bin, a placing bin, a mixing bin, protective cotton, a test tube groove, a comparison test tube, a rotary cylinder, a motor, a power supply, a control button, a partition plate and a detection test tube, wherein the application number of the kit is CN 201820189385.4.

The gene detection kit based on the nano magnetic bead method can fully mix the reagent and the sample, but the gene detection kit based on the nano magnetic bead method can not realize separation of magnetic beads and a solution and can not realize subsequent elution operation.

Disclosure of Invention

The utility model provides a gene sequencing kit, which solves the problem that magnetic beads cannot be automatically separated from a solution in the prior art.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model discloses a gene sequencing kit, which comprises: the bottom box body is provided with at least two storage tanks, the storage tanks are used for storing reaction substances, one storage tank is a magnetic bead separation tank, an inclined guide wall is formed at the bottom of the magnetic bead separation tank, one side wall of the magnetic bead separation tank is an adsorption wall, the inclined guide wall is used for guiding magnetic beads to the adsorption wall, an adsorption execution block is installed outside the bottom box body and fixed to the output end of the pushing mechanism, the pushing mechanism is used for driving the adsorption execution block to be far away from or close to the magnetic bead separation tank, and the adsorption execution block is located on one side of the adsorption wall, which is far away from the inclined guide wall.

Preferably, all the storage tanks are distributed on a circumference, and the inclined guide wall is positioned on one side of the adsorption wall away from the center of the circumference.

Preferably, the bottom wall in the magnetic bead separation groove is a needle opposite wall, and the needle opposite wall is of a circular structure.

Preferably, the adsorption wall is of a partial cylindrical surface structure.

Preferably, the bottom of the bottom box body extends downwards to form a storage container, and a storage groove is formed in the storage container.

Preferably, the connection root of the storage container and the bottom box body is provided with a reinforcing rib in a downward protruding mode.

Preferably, the central bottom of the bottom box body extends downwards to form a guide cylinder, and the guide cylinder is used for guiding the upper rotating cylinder to rotate.

Preferably, the edge of the bottom surface of the bottom box body protrudes downwards to form a bottom ring.

Preferably, the bottom ring is connected with the storage containers through reinforcing ribs, the guide cylinder is connected with the storage containers through reinforcing ribs, and the two adjacent storage containers are connected through reinforcing ribs.

Compared with the prior art, the utility model has the following beneficial effects:

in this application, in order to realize automatic imbibition to appointed holding tank after, the certain biological reaction of time in the holding tank of different reagents has consequently set up end box body center and has set up the guiding hole, is equipped with the round of holding tank outward in the guiding hole, can align with every holding tank in proper order after the imbibition head is rotatory, just so can absorb or discharge to the interior liquid of every holding tank. And set up a holding tank as a magnetic bead spread groove in this application, realized when magnetic bead and liquid need separate after mixing, the absorption execution piece is close to the adsorption wall, the absorption execution piece adopts magnet material to make, because the effect of slope guide wall for the magnetic bead of less granule has the trend of directional adsorption wall motion under self gravity condition, because if the magnetic bead spread groove is more even, far away from the absorption execution piece, the adsorption dynamics is insufficient, rely on the guide effect of slope guide wall, can make the magnetic bead all adsorbed, adsorb after adsorbing on the adsorption wall like this, the liquid in the magnetic bead spread groove is inhaled to the imbibition head, then with magnetic bead and liquid separation, the magnetic bead can not follow the removal together in the time of making imbibition, thereby make the magnetic bead can not be absorbed, guarantee that the magnetic bead that contains the gene fragment can not be absorbed away, guarantee that follow-up can conveniently detect.

Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the utility model.

Drawings

FIG. 1 is a schematic diagram of the structure of a gene sequencing kit.

Fig. 2 is an enlarged view at a in fig. 1.

FIG. 3 is a bottom view of the gene sequencing kit.

FIG. 4 is a B-B cross-sectional view of the gene sequencing kit of FIG. 3.

Reference numerals: the magnetic bead separation device comprises a bottom box body 1, a storage tank 10, a magnetic bead separation tank 11, an inclined guide wall 111, an adsorption wall 112, a needle alignment wall 113, reinforcing ribs 2, a guide cylinder 3 and a bottom ring 4.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the present utility model more clear and easy to understand, the present utility model is further described below with reference to the accompanying drawings and the detailed description:

as shown in fig. 1 to 4, the present utility model discloses a gene sequencing kit, comprising: the bottom box body 1 is provided with at least two storage tanks 10, the storage tanks 10 are used for storing reaction substances, one storage tank 10 is a magnetic bead separation tank 11, an inclined guide wall 111 is formed at the bottom of the magnetic bead separation tank 11, one side wall of the magnetic bead separation tank 11 is an adsorption wall 112, the inclined guide wall 111 is used for guiding magnetic beads to the adsorption wall 112, an adsorption execution block is arranged outside the bottom box body 1 and fixed to the output end of a pushing mechanism, the pushing mechanism is used for driving the adsorption execution block to be far away from or close to the magnetic bead separation tank 11, and the adsorption execution block is located on one side of the adsorption wall 112 away from the inclined guide wall 111.

All the storage tanks 10 are distributed on a circumference, and the inclined guide wall 111 is located on the side of the suction wall 112 facing away from the center of the circumference. Conveniently, the pipette head can be aligned with each storage slot 10. Since a large number of storage tanks 10 are provided on the circumference, the inclined guide wall 111 is provided on the side of the adsorption wall 112 facing away from the center of the circumference, so that the adsorption execution block is not blocked by the other storage tanks 10 and can be sufficiently contacted with the adsorption wall 112.

The inner bottom wall of the magnetic bead separation groove 11 is a needle facing wall 113, and the needle facing wall 113 has a circular structure. Due to the arrangement of the inclined guide wall 111, the area of the needle wall 113 is smaller, so that a large amount of mixed liquid can be stored, and meanwhile, the phenomenon that the magnetic beads at a far place cannot be adsorbed on the adsorption wall 112 due to the fact that the needle wall 113 is too large is avoided.

The adsorption wall 112 has a partial cylindrical surface structure. Since there are many storage tanks 10 adjacent to each other, the width of the magnetic bead separation tank 11 is limited, and the design of the partial cylindrical surface structure makes the adsorption wall 112 larger, so that the adsorption wall 112 can fully adsorb the magnetic beads after fully contacting the adsorption execution block.

The bottom of the bottom box body 1 extends downwards to form a storage container, and a storage groove 10 is arranged in the storage container. The bottom box body 1 needs to be arranged on the mounting seat in a counterpoint mode, so that the storage container at the bottom of the bottom box body 1 is convenient to position, and due to the fact that the storage groove 10 is deeper, the material consumption of the bottom box body 1 can be reduced, and the cost is reduced.

The connection root of the storage container and the bottom box body 1 is provided with a reinforcing rib 2 which protrudes downwards. The reinforcing ribs 2 are arranged, so that the root strength of the storage container is enough, and deformation damage caused by bending of the root of the storage container during demolding is avoided.

The bottom of the center of the bottom box body 1 extends downwards to form a guide cylinder 3, and the guide cylinder 3 is used for guiding the upper rotating cylinder to rotate. The guide cylinder 3 is provided to guide the rotation of the mounting base (rotary cylinder) of the upper liquid suction head.

The edge of the bottom surface of the bottom box body 1 protrudes downwards to form a bottom ring 4. The bottom ring 4 provides the basis for the subsequent formation of the reinforcing bars 2.

The bottom ring 4 is connected with the storage containers through the reinforcing ribs 2, the guide cylinder 3 is connected with the storage containers through the reinforcing ribs 2, and the two adjacent storage containers are connected through the reinforcing ribs 2. So that the whole strength of the bottom box body 1 is enough, and the strength of the root of the storage container is further enhanced.

Finally, it is noted that the above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present utility model, which is intended to be covered by the scope of the claims of the present utility model.

Claims

1. A gene sequencing kit, comprising: the bottom box body (1) is provided with at least two storage tanks (10), the storage tanks (10) are used for storing reaction substances, one storage tank (10) is a magnetic bead separation tank (11), an inclined guide wall (111) is formed at the bottom of the magnetic bead separation tank (11), one side wall of the magnetic bead separation tank (11) is an adsorption wall (112), the inclined guide wall (111) is used for guiding magnetic beads to the adsorption wall (112), an adsorption execution block is arranged outside the bottom box body (1), the adsorption execution block is fixed to the output end of a pushing mechanism, the pushing mechanism is used for driving the adsorption execution block to be far away from or close to the magnetic bead separation tank (11), and the adsorption execution block is located on one side of the adsorption wall (112) deviating from the inclined guide wall (111).

2. The gene sequencing kit according to claim 1, wherein all the storage grooves (10) are distributed on a circumference, and the inclined guide wall (111) is located on the side of the adsorption wall (112) facing away from the center of the circumference.

3. The gene sequencing kit according to claim 2, wherein the inner bottom wall of the magnetic bead separation groove (11) is a needle facing wall (113), and the needle facing wall (113) is of a circular structure.

4. A gene sequencing kit according to claim 3, characterized in that the adsorption wall (112) is of partial cylindrical surface structure.

5. The gene sequencing kit according to any one of claims 1 to 4, wherein the bottom of the kit body (1) extends downwards to form a storage container, and the storage container is internally provided with a storage groove (10).

6. The gene sequencing kit according to claim 5, wherein the connection root of the storage container and the kit body (1) is protruded with a reinforcing rib (2) downward.

7. The gene sequencing kit according to claim 6, wherein the bottom of the center of the kit body (1) extends downward to form a guide cylinder (3), and the guide cylinder (3) is used for guiding the upper rotating cylinder to rotate.

8. The gene sequencing kit according to claim 7, wherein the bottom edge of the bottom case body (1) protrudes downward to form a bottom ring (4).

9. The gene sequencing kit according to claim 8, wherein the bottom ring (4) is connected with the storage containers through the reinforcing ribs (2), the guide cylinder (3) is connected with the storage containers through the reinforcing ribs (2), and the two adjacent storage containers are connected through the reinforcing ribs (2).