CN220018800U - Laboratory device for monitoring pressure and fermentation process - Google Patents
Laboratory device for monitoring pressure and fermentation process Download PDFInfo
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- CN220018800U CN220018800U CN202321582326.0U CN202321582326U CN220018800U CN 220018800 U CN220018800 U CN 220018800U CN 202321582326 U CN202321582326 U CN 202321582326U CN 220018800 U CN220018800 U CN 220018800U
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- 238000000855 fermentation Methods 0.000 title claims abstract description 152
- 230000004151 fermentation Effects 0.000 title claims abstract description 151
- 238000012544 monitoring process Methods 0.000 title claims abstract description 28
- 235000000346 sugar Nutrition 0.000 claims abstract description 61
- 230000008859 change Effects 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims description 10
- 235000013361 beverage Nutrition 0.000 claims description 4
- 239000007788 liquid Substances 0.000 abstract description 26
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 20
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 10
- 239000001569 carbon dioxide Substances 0.000 abstract description 10
- 238000004364 calculation method Methods 0.000 abstract description 5
- 238000011160 research Methods 0.000 abstract description 5
- 230000034659 glycolysis Effects 0.000 abstract description 4
- 239000004615 ingredient Substances 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 20
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 20
- 239000007789 gas Substances 0.000 description 20
- 238000000034 method Methods 0.000 description 10
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 235000014101 wine Nutrition 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
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- 229920002472 Starch Polymers 0.000 description 1
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- 235000013336 milk Nutrition 0.000 description 1
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Abstract
The utility model provides a laboratory device for monitoring pressure and fermentation process, which is used for monitoring the pressure of a glycolysis system and the fermentation process of the glycolysis system in real time, is simple and easy to use and is feasible, and comprises a fermentation tank, a pressure sensor and a pressure control valve, wherein the fermentation tank is used for containing sugar liquid; the pressure control valve punctures a puncture on the cover of the fermentation tank; the pressure sensor is arranged on the tank cover of the fermentation tank, is communicated with the interior of the fermentation tank through the puncture and is used for detecting the pressure in the fermentation tank in real time so as to reflect the fermentation degree of the sugar liquid. During operation, sugar liquid ingredients are injected into the fermentation tank, the fermentation tank is connected to the monitoring system, the fermentation tank is placed in the incubator for fermentation, and the pressure sensor detects the gas pressure value in the system in real time. The pressure change is monitored in real time through the sensor, and according to the pressure change condition and the calculation of an ideal gas equation, the generation condition of the carbon dioxide gas quality can be obtained, and the sugar solution has wide application value in research and judgment.
Description
Technical Field
The utility model relates to a laboratory device for monitoring pressure and fermentation process, and relates to the technical field of sugar liquor yeast ethanol anaerobic fermentation process and pressure real-time monitoring thereof.
Background
The wine can be divided into white wine, yellow wine, beer, fruit wine, and compound wine, among which the most important steps are to utilize distiller's yeast or yeast to convert the carbohydrate such as starch and sugar in the raw materials (such as grains and fruits) into a series of substances such as ethanol through a series of biological transformations. The anaerobic fermentation process involving the aerobic and anaerobic fermentation process mechanisms of yeast in this step, the anaerobic fermentation process (edible) for ethanol production is the only industrial process for ethanol production for a considerable historical period.
In the fermentation process of alcohol or wine by the traditional liquid method, the fermentation degree of sugar liquor can be judged by visual inspection, smell, try and other methods, and the methods require operation management personnel to have more experience accumulation and have certain subjectivity and uncertainty. The fermentation degree of the sugar solution can be accurately judged through chemical detection of the alcohol degree, the change of the sugar content and the like of a measuring system, but the detection of the alcohol degree of the fermentation solution and the analysis of the sugar component of the fermentation solution by a high-liquid chromatography have higher requirements on instruments and operators, are time-consuming and labor-consuming, require higher cost, and can destroy and change the original sealed fermentation environment during sampling. The latter is especially unfavorable for the research of a small-volume fermentation system in a laboratory, the original fermentation process is disturbed and changed due to the intermediate sampling, and the fermentation is continuously performed according to new environmental conditions; with reference to a normal fermentation process in which no sample is taken from beginning to end, the more the number of intermediate samples, the more the process route that deviates from the original fermentation should be changed.
In Journal of Chemical education 2018,95,828-832, a self-made simple water-driven volumetric gas meter is used, which consists of a plastic water bottle, a plastic tube and a graduated cylinder, and is used for measuring the evolution of carbon dioxide when baker's yeast ferments sugar. The device is capable of providing reproducible quantitative data that can be plotted to show induction lag time, steady state growth, and nutrient depletion, and can be used to demonstrate the effect of various conditions (e.g., carbohydrate type and concentration, inhibitors, pH and temperature) on yeast fermentation kinetics. The technology utilizes the scale cylinder to measure the gas volume, so that the general trend of the yeast fermentation sugar liquid can be observed rapidly, intuitively and simply, but systematic errors and manual reading errors exist when the scale cylinder reads data, and the experimental data and results can be influenced.
Disclosure of Invention
In the anaerobic fermentation process of sugar liquid, which is generally carried out at the temperature of 20-40 ℃, sugar is continuously fermented into ethanol and carbon dioxide, the pressure of a tank system is increased continuously along with the fermentation, and the residual sugar is reduced continuously; when the concentration of ethanol in the system is continuously increased and the concentration of residual sugar is continuously reduced to a certain degree, the inhibition of the ethanol product is more and more obvious, the concentration content of fermentable sugar is reduced to a degree that yeast is difficult to use, and the sugar fermentation of the yeast is basically completed in a terminated state. In the whole fermentation process, along with the continuous generation of carbon dioxide, the pressure of gas (mainly carbon dioxide) in a closed system is also continuously increased; as the concentration of fermentable sugars decreases, the increase in gas pressure in the fermentation system slows, stabilizes and no longer increases or even decreases, meaning that the fermentation of the system has substantially ceased to complete.
In the state that the carbon dioxide belongs to ideal gas under the conditions of normal temperature (the optimal temperature range of yeast fermentation: 25-35 ℃) and low pressure (no external pressure is applied) in the temperature and pressure range, PV=nRT (wherein P is the pressure value displayed by a pressure meter; V is the volume of liquid removed in a fermentation tank; n is the quantity of substances of consumed sugar; R is an ideal gas constant; and T is the fermentation temperature) can be used for representing, therefore, the device provided by the embodiment monitors the increase and the change of the air pressure in the system in real time on line, and under the condition that the volume of the upper space of the liquid level of the sugar liquid in the cola bottle fermentation tank is obtained by measurement, the quantity and quality of carbon dioxide generated in real time or in unit time can be obtained by calculation of the air state equation, and then the time change result of the carbon dioxide molar concentration in the system can be obtained by calculation of the reaction type, and the initial fermentation sugar liquid (comprising the concentration and the volume of the sugar liquid) in real time or in unit time can be obtained, so that the dynamic change result of the molar concentration of the residual sugar of the fermentation liquid in the fixed experiment can be obtained, and information of the fermentation equation of the sugar liquid fermentation can be deduced, and the like are simple, safe, the operation, low, the reliability and the accuracy of the fermentation degree can be realized.
The utility model provides a device system for monitoring gas pressure of a fermentation system for a laboratory, which is used for monitoring the pressure of yeast glycolysis sugar solution converted into ethanol and the fermentation process of the yeast glycolysis sugar solution, and comprises a fermentation tank, a pressure sensor and a pressure control valve,
the fermentation tank is used for containing sugar solution;
the pressure control valve punctures a puncture on the cover of the fermentation tank;
the pressure sensor is arranged on the tank cover of the fermentation tank, is communicated with the interior of the fermentation tank through the puncture and is used for detecting the pressure in the fermentation tank in real time so as to reflect the fermentation degree of the sugar liquid.
Further, the fermentation tank adopts a gas-containing beverage bottle.
Further, the fermentation tank is a plastic bottle.
Further, the fermentation tank is a plastic cola bottle.
Further, the pressure sensor comprises at least one display screen for displaying the pressure value of the current sugar liquid fermentation degree and the working state of the whole device.
Further, the fermentation tank also comprises a base, and the fermentation tank is arranged on the base.
Further, the buffer tube is further provided, two ends of the buffer tube are respectively connected with a pressure sensor and a fermentation tank, and the pressure sensor is communicated with the fermentation tank through the puncture opening and the buffer tube.
Further, the pressure sensor adopts a digital display type pressure gauge.
Further, the device also comprises a bracket, and the pressure control valve is arranged on the bracket.
Further, the pressure control valve comprises a handle and a puncture needle, wherein the handle is hinged to the support, one end of the puncture needle is fixed to the handle, and when the handle is rotated, the other end of the puncture needle can puncture the tank cover of the fermentation tank.
Further, the pressure control valve further comprises a sealing ring.
The pressure sensor is used for monitoring the change of the gas pressure in the fermentation tank in real time. The specific implementation method comprises the following steps:
1. when the fermenter (e.g. plastic cola bottle) is full of distilled water below the fermentation temperature, the distilled water is poured out and weighed, the density of the water below the fermentation temperature is queried, and the volume of the fermenter used is calculated.
2. Preparing sugar solution with concentration of 20%, adding 0.2% of nutrient elements such as organic nitrogen source and mineral substance, and 0.1% of yeast, and stirring uniformly (wherein the sugar solution concentration, organic nitrogen source and yeast addition can be changed according to self experimental condition requirements).
3. Taking up the volume of the fermenterIs put into a fermentation tank, shaken uniformly, sealed and connected to a pressure sensor, and is put into a biochemical incubator (the volume of the sugar liquid is preferably kept at +.>To->Preferably not exceeding the volume of the fermenter>)。
4. And starting the pressure sensor and the controller, timing, and starting to monitor and record the pressure and the change of the pressure in the fermentation tank.
5. According to the pressure value change condition, the fermentation degree and change of the sugar solution can be timely, accurately, safely and reliably known through calculation of an ideal gas equation of PV=nRT.
For the whole process monitoring detection research of the alcoholic fermentation system with small laboratory volume, the utility model can take the common standardized plastic bottles of cola, bacea or other gas-containing beverages as fermentation containers, develop and design a practical and simple experimental device for teaching experiments and scientific research experiments in the laboratory, does not need any can opening and bottle opening sampling, does not damage the fermentation passing state of the system, and can monitor the pressure and the change of the fermentation system at any time when the whole process of yeast fermentation of sugar liquor keeps original ecology change. When the pressure of the closed fermentation system is continuously increased, the increase is slowed down, and the pressure of the system is not increased or even reduced any more until the system pressure is stable, the fermentation of the system can be considered to be basically stopped and completed at the moment.
The utility model monitors the pressure value by using the digital display pressure gauge, calculates the consumption of sugar in the sugar liquid by using an ideal gas state equation, improves the precision of an instrument device and the stability of experimental data, and can obtain more accurate trend of the yeast fermentation sugar liquid.
Drawings
FIG. 1 is a schematic diagram of a fermentation change curve of a sugar solution.
Fig. 2 is a schematic structural diagram of a laboratory device for monitoring pressure and fermentation process according to an embodiment of the present utility model.
Fig. 3 is a design diagram of the device according to the present embodiment when the pressure is too high.
The figure shows: 1 is a pressure control valve, 2 is a pressure sensor, 3 is a bracket, 4 is a fermentation tank, 5 is a buffer tank, and 6 is a base.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The laboratory device for monitoring the pressure of the sugar liquid yeast anaerobic fermentation system and the fermentation process thereof in real time can be used for monitoring the fermentation process of the sugar liquid yeast, can be used for conveniently judging the fermentation degree of the sugar liquid by detecting the pressure and the change of the gas in the fermentation process system, and has the characteristics of safety, reliability and simplicity in operation. The device comprises a fermentation tank 4, a pressure sensor 2 and a pressure control valve 1. The fermentation tank 4 is internally provided with sugar liquid, the pressure sensor 2 is connected with the fermentation tank 4 and is communicated with the interior of the fermentation tank 4, and the device is in a vertical placement state, so that the sugar liquid is prevented from being in direct contact with the pressure sensor 2. The pressure sensor 2 detects the pressure change in the fermentation tank 4, so as to judge the fermentation degree of the sugar solution.
The pressure sensor 2 is communicated with the inner cavity of the fermentation tank 4. The pressure sensor 2 can detect carbon dioxide gas generated in the fermentation process of the sugar solution in the fermentation tank 4 in real time, and convert the carbon dioxide gas into a pressure value so as to reflect the fermentation degree of the sugar solution.
In some of the embodiments of the utility model, the fermenter 4 is a reusable container that can be used to maintain temperature and pressure during fermentation while having cleanable and sterilizable properties.
In some embodiments of the present utility model, the fermenter 4 may be a gas-containing beverage bottle such as cola.
In some of the embodiments of the utility model, as shown in fig. 3, the apparatus further comprises a base 6, the fermenter 4 being provided on the base 6.
In some of the embodiments of the utility model, as shown in fig. 2 and 3, the device further comprises a support 3, and the pressure control valve 1 is arranged on the support 3.
The pressure control valve 1 comprises a handle, a puncture needle and a sealing ring, wherein the handle is hinged to the support 3, one end of the puncture needle is fixed on the handle, when the handle is pressed and rotated, the other end of the puncture needle can puncture the tank cover of the fermentation tank 4, the sealing ring is wrapped outside the puncture needle, and the sealing ring is tightly contacted with the plane of the bottle cover along with the pushing process of the handle. The bottle cap of the fermentation tank 4 is pierced by a puncture needle in a mechanical pressing mode, the pressure sensor 2 is communicated with the fermentation tank 4, and a closed detection environment is achieved under the action of a sealing ring. Along with the continuous increase of pressure, the sealing ring is tightly pressed, a sealing environment is formed at the joint of the sealing ring and the bottle cover, and the puncture needle can not be compressed at the moment but directly puncture the bottle cover to form a communication environment of the pressure gauge and the fermentation tank.
In some of these embodiments of the utility model, the device is placed in a temperature-stabilized incubator (e.g., an incubator at 28 ℃) and fermentation is performed at constant temperature, and the device includes a display screen for displaying real-time pressure data.
In some embodiments of the utility model, the pressure sensor is a digital display pressure gauge.
In some embodiments of the present utility model, as shown in fig. 3, if the pressure value changes greatly, which would damage the pressure sensor 2, a buffer tube 5 may be added between the pressure sensor 2 and the fermenter 4 to reduce the great impact of the pressure on the pressure sensor 2.
When the utility model is implemented, an operator firstly pours sugar liquid into the fermentation tank 4, the fermentation tank bottle cap is pierced by a mechanical pressing mode, the fermentation tank 4 is tightly connected with the pressure sensor 2, the pressure sensor 2 is started to record after no air leakage condition occurs, and the purpose of guaranteeing the stability and quality of fermentation is achieved according to real-time pressure value judgment.
The ideal gas equation pv=nrt can be used to calculate and derive a sugar liquor fermentation degree judgment curve, as shown in fig. 1. Note: the utility model monitors the fermentation process, not the fermentation quality, through the gas pressure of the system
In some embodiments of the utility model, in actual use, firstly, a brown granulated sugar solution with the concentration of 20% (w/v) is prepared, 1.0% of sugar-free milk powder is dissolved, 0.75% of sugar-free Angel white spirit yeast is added, stirring and dispersing are carried out, then 200 ml of a certain sugar solution is measured, 550 ml of cola variety is added, then the pressure sensor 2 is connected with the fermentation tank 4 (such as a cola bottle) through mechanical pressing of the control valve 1, after the tightness is ensured, the biochemical incubator with the temperature of 28 ℃ is opened, and the pressure sensor 2 is recorded. Along with the fermentation, the pressure sensor 2 continuously monitors the pressure change in the fermentation tank, displays the pressure change through a digital display screen, records the pressure value on the digital display screen at daily time, achieves the purpose of monitoring the pressure in real time according to the daily recorded value, calculates according to an ideal gas state equation PV=nRT, and timely, accurately, safely and reliably knows the fermentation degree and the change of the sugar solution.
200 ml of 20% brown granulated sugar and liquid fermentation broth are filled into 550 ml clean cola bottles, and fermented for 13 days in a constant temperature closed environment at 28 ℃, and the change curves of the pressure value, the alcohol degree and the sugar consumption of the system are shown in figure 1. As can be seen from fig. 1, the pressure of the system is continuously increased, the amplitude is reduced, the pressure is gradually flattened, and the variation trend of the three curves is the same.
The laboratory device for monitoring the pressure and the fermentation process of the pressure provided by the embodiment of the utility model can judge and know the fermentation degree of the sugar solution through the change of the pressure value, is suitable for student teaching experiments and scientific research experiments, is used for small-scale experiments for improving and optimizing the production process by alcohol enterprises in winery, has the characteristics of safety, reliability, simplicity in operation, high efficiency, cost and the like, and has wide application prospects.
The laboratory device for monitoring the pressure and the fermentation process of the pressure has the following advantages:
(1) Online real-time and scalability: and (3) starting fermentation timing after the sealing condition is finished, and stopping fermentation of the system without opening the bottle and the tank, wherein the pressure sensor is used for detecting the system on line and monitoring in real time. If the pressure sensor is connected with a storage display device of a printer or a computer, a change curve of the pressure in a system in the fermentation process can be recorded, parameters such as the upper volume of a fermentation tank, the concentration of sugar liquid and the like are input, and the details of the fermentation process can be known and the expansibility can be realized through a series of calculation steps such as an ideal gas equation and the like.
(2) The precision is high: the pressure change in the fermentation process can be monitored and calculated in real time through accurate recording of the digital display pressure sensor, and the detection precision and reliability are improved.
(3) The operation is simple and convenient: the whole system is quite simple to operate, only the installation of the device is needed, the air tightness is ensured, and the operation of a professional technician is not needed.
(4) Safety and reliability: the fermentation tank that this device adopted can adopt the common soda bottle in market, and the soda bottle is the standardized product that circulates in the market, and withstand voltage is effectual, and product quality guarantees to some extent, can ensure stability and the security of system in the fermentation process, has reduced the risk in the operation process.
(5) The practicability is strong, and the application range is wide: the device provided by the utility model can be used for researching the fermentation process of the sugar solution on line in real time under different conditions of different fermentation raw materials (various sugar, nitrogen sources, mineral substances, yeast and the like and proportions thereof) in a laboratory, different sugar solution concentrations, fermentation temperatures, initial pH sugar solution of the fermentation solution and the like. The method is used as a small test in alcohol or fruit wine production enterprises, is used for the test acceptance of raw materials and the improvement and optimization of fermentation production processes, and has practical and economic values.
(6) The cost is low: the components selected by the device are all standardized products circulated in the market, the device is low in cost and operation, easy to construct and suitable for large-scale teaching experiments in laboratories.
It is to be understood that the above examples of the present utility model are provided by way of illustration only and not by way of limitation of the embodiments of the present utility model. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are desired to be protected by the following claims.
Claims (10)
1. The laboratory device for monitoring the pressure and the fermentation process in real time is characterized by comprising a fermentation tank (4), a pressure sensor (2) and a pressure control valve (1),
the fermentation tank (4) is used for containing sugar solution;
the pressure control valve (1) punctures a puncture on the cover of the fermentation tank (4);
the pressure sensor (2) is arranged on a tank cover of the fermentation tank (4), is communicated with the interior of the fermentation tank (4) through the puncture and is used for detecting the gas pressure change in the fermentation tank (4) in real time so as to reflect the fermentation degree of the sugar solution.
2. Laboratory device for the real-time monitoring of pressure and fermentation processes according to claim 1, characterized in that the fermenter (4) is a gas-containing beverage bottle.
3. Laboratory device for the real-time monitoring of pressure and fermentation processes according to claim 1, characterized in that the fermenter (4) is a plastic bottle.
4. The laboratory device for monitoring the pressure and fermentation process in real time according to claim 1, wherein the pressure sensor comprises at least one display screen for displaying the pressure value of the current sugar liquor fermentation degree and the working state of the whole device.
5. Laboratory device for the real-time monitoring of pressure and fermentation processes according to claim 1, characterized in that it further comprises a base (6), said fermenter (4) being arranged on said base (6).
6. The laboratory device for monitoring pressure and fermentation process in real time according to claim 1, further comprising a buffer tube (5), wherein the two ends of the buffer tube (5) are respectively connected with a pressure sensor (2) and a fermentation tank (4), and the pressure sensor (2) is communicated with the fermentation tank (4) through the puncture opening and the buffer tube (5).
7. Laboratory device for the real-time monitoring of pressure and fermentation processes according to claim 1, characterized in that the pressure sensor (2) is a digital display type pressure gauge.
8. Laboratory device for the real-time monitoring of pressure and fermentation processes according to any one of claims 1-7, characterized in that it further comprises a support (3), said pressure control valve (1) being arranged on the support (3).
9. Laboratory device for the real-time monitoring of pressure and fermentation processes according to claim 8, characterized in that the pressure control valve (1) comprises a handle hinged to the support (3) and a spike, one end of which is fixed to the handle, the other end of which is able to pierce the cover of the fermenter (4) when the handle is turned.
10. Laboratory device for the real-time monitoring of pressure and fermentation processes according to claim 9, characterized in that the pressure control valve (1) further comprises a sealing ring, which is arranged on the outer wall of the spike.
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| CN202321582326.0U CN220018800U (en) | 2023-06-20 | 2023-06-20 | Laboratory device for monitoring pressure and fermentation process |
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| CN202321582326.0U CN220018800U (en) | 2023-06-20 | 2023-06-20 | Laboratory device for monitoring pressure and fermentation process |
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| CN220018800U true CN220018800U (en) | 2023-11-14 |
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