CN219861377U - Cell proliferation differentiation's culture apparatus - Google Patents
Cell proliferation differentiation's culture apparatus Download PDFInfo
- Publication number
- CN219861377U CN219861377U CN202321052358.XU CN202321052358U CN219861377U CN 219861377 U CN219861377 U CN 219861377U CN 202321052358 U CN202321052358 U CN 202321052358U CN 219861377 U CN219861377 U CN 219861377U
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- Prior art keywords
- culture tank
- penetrating
- base
- culture
- cell proliferation
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- 230000004663 cell proliferation Effects 0.000 title claims abstract description 14
- 230000004069 differentiation Effects 0.000 title description 3
- 230000000149 penetrating effect Effects 0.000 claims abstract description 54
- 230000024245 cell differentiation Effects 0.000 claims abstract description 12
- 230000007246 mechanism Effects 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims description 16
- 230000035515 penetration Effects 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 8
- 230000037431 insertion Effects 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims 2
- 238000000034 method Methods 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 210000005258 dental pulp stem cell Anatomy 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 210000003074 dental pulp Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 210000004262 dental pulp cavity Anatomy 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell proliferation and differentiation culture device, which comprises: the culture tank is internally provided with a lifting assembly in a sliding manner, the bottom end of the lifting assembly is connected with an inserting rod, and the inserting rod is connected with the bottom of the culture tank in an inserting manner; external threads are formed outside the culture tank, a base is sleeved on the external threads of the culture tank, the bottom end of the penetrating rod is connected with a mounting device, and the mounting device is connected with the base in a penetrating manner; the mounting device comprises a penetrating pipe, a limiting ring and an elastic positioning mechanism. According to the utility model, by utilizing the matched arrangement mode of the culture tank and the base, the mounting device can neutralize the rotation motion in the threaded motion of the base through the interpenetration between the mounting device and the base, and the linear motion part is reserved, so that the interpenetration rod pushes the lifting component to lift up in the culture tank, and the probability of toppling is reduced due to the large specific surface area of the bottom end of the base, so that the culture device is stable when being placed on a workbench.
Description
Technical Field
The utility model relates to the field of culture devices, in particular to a culture device for cell proliferation and differentiation.
Background
The dental pulp tissue is positioned in the dental pulp cavity in the tooth and is the only soft tissue in the dental tissue, the fibroblast separated from the dental pulp tissue is called dental pulp stem cells, the dental pulp stem cells have the potential of multidirectional differentiation, and besides cells capable of forming mineralization junction capacity, the dental pulp stem cells can be differentiated into cell line types such as fat, bone, cartilage, muscle, vascular endothelium, liver, nerve and the like through the induction of different cytokines, so that the culture of the dental pulp stem cells has very far-reaching significance.
The cell culture who carries out among the conventional operation utilizes conventional culture dish to operate more, general culture dish simple structure, because the culture tank of culture dish has certain degree of depth, when cell inoculation, operating personnel's use impression is not good, cell sampling after the cultivation is accomplished also difficult operation, so in prior art, patent number 202121429221.2 name is the dental pulp stem cell culture device of being convenient for observe, wherein, the inside culture tank that is provided with of device body, the culture tank bottom is provided with the connecting axle, the connecting axle stretches out from the connector, the connecting axle body surface is provided with the screw thread, the accessible swivelling joint axle body is raised the culture tank, conveniently observe the inside condition of culture tank, the counter swivelling joint axle body then can descend the culture tank.
However, in the above patent, although the height of the culture tank is adjusted by the threaded insertion of the connecting shaft, the surface area of the bottom of the connecting shaft is small, and when the device is placed on the workbench and directly supported by the connecting shaft, the device is in a state of large top and small bottom, so that the device is not stable enough and is easy to topple over.
Disclosure of Invention
The present utility model is directed to a cell proliferation and differentiation culture apparatus, which solves the above-mentioned problems of the prior art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: a cell proliferation and differentiation culture device comprising:
the culture tank is internally provided with a lifting assembly in a sliding manner, the bottom end of the lifting assembly is connected with an inserting rod, and the inserting rod is connected with the bottom of the culture tank in an inserting manner;
external threads are formed outside the culture tank, a base is sleeved on the external threads of the culture tank, the bottom end of the penetrating rod is connected with a mounting device, and the mounting device is connected with the base in a penetrating manner;
the mounting device comprises a penetrating pipe, a limiting ring and an elastic positioning mechanism, wherein the penetrating pipe is connected with the base in a penetrating mode, the limiting ring is sleeved outside the penetrating pipe, and the elastic positioning mechanism is connected with the penetrating pipe in a penetrating mode.
Preferably, the top end of the culture tank is connected with a connecting port, an external thread is arranged on the outer wall of the connecting port, and a sealing cover is sleeved on the external thread of the connecting port.
Preferably, the lifting assembly comprises a culture tank and a sealing ring, the culture tank is arranged in the culture tank in a sliding mode, the sealing ring is fixedly sleeved outside the culture tank, and the outer wall of the sealing ring is attached to the inner wall of the culture tank.
Preferably, a rectangular groove is formed in the bottom of the culture tank, and the penetrating rod is mutually penetrated and matched with the inner cavity of the rectangular groove.
Preferably, the bottom of base has seted up the counter bore, the through-hole has been seted up on the top of counter bore, alternate pipe and the inner chamber slip alternate connection of through-hole, the spacing ring activity sets up in the inside of counter bore.
Preferably, the elastic positioning mechanism comprises a limiting slide plate, a limiting clamping block and a spring, wherein the two limiting slide plates are symmetrically arranged in the penetrating pipe, the spring is connected to the top between the two limiting slide plates, the two limiting clamping blocks are respectively connected to one sides of the two limiting slide plates, which are opposite, and the limiting clamping blocks are connected with the bottom of the penetrating pipe in a sliding penetrating manner.
Preferably, a guide rod is movably arranged in the spring, the limiting sliding plate is connected with the top of the guide rod in a sliding penetrating manner, and the end part of the guide rod is fixedly connected with the inner wall of the penetrating pipe.
The utility model has the technical effects and advantages that:
(1) According to the utility model, by utilizing the matched arrangement mode of the culture tank and the base, the mounting device can neutralize the rotation motion in the threaded motion of the base through the insertion between the mounting device and the base, and the linear motion part is reserved, so that the insertion rod pushes the lifting component to lift up in the culture tank, the lifting component can be lifted up in the culture tank by a certain height, the tissue sample cultured on the lifting component can be conveniently observed in a short distance, and the probability of dumping is reduced due to the larger specific surface area of the bottom end of the base, so that the culture device is stable when being placed on a workbench;
(2) According to the utility model, by means of the arrangement mode that the limiting slide plates and the limiting clamping blocks are matched, the two limiting slide plates are elastically supported through compression deformation of the springs, so that penetration between the limiting clamping blocks and the penetration pipe is more stable, the limiting clamping blocks are used for limiting the position of the limiting ring, the limiting ring is used for limiting the position of the penetration pipe, the penetration pipe is prevented from moving upwards relative to the base, the base is enabled to rotate more stably relative to the mounting device, the sliding of the limiting slide plates is facilitated to be limited through penetration between the limiting slide plates and the guide rods, and the linear sliding of the limiting slide plates is enabled to be more stable.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model.
FIG. 2 is a schematic diagram of the internal front structure of the present utility model.
FIG. 3 is a schematic diagram of a second front view of the present utility model.
Fig. 4 is a schematic view showing the bottom view of the insertion rod according to the present utility model.
Fig. 5 is a schematic view of the front structure of the mounting device of the present utility model.
FIG. 6 is a schematic view showing the internal structure of the mounting device of the present utility model.
In the figure: 1. a culture tank; 11. a connection port; 2. a lifting assembly; 21. a culture tank; 22. a seal ring; 3. penetrating the inserting rod; 4. a base; 5. a mounting device; 51. a penetrating pipe; 52. a limiting ring; 53. an elastic positioning mechanism; 531. a limit sliding plate; 532. a limit clamping block; 533. a spring; 6. sealing cover.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The present utility model provides a cell proliferation and differentiation culture apparatus as shown in FIGS. 1 to 6, comprising:
the culture tank 1, the top end of the culture tank 1 is connected with a connecting port 11, the outer wall of the connecting port 11 is provided with external threads, the external threads of the connecting port 11 are sleeved with a sealing cover 6, and the sealing of the culture tank 1 is facilitated through the sealing cover 6;
the inside slip of cultivateing jar 1 is provided with lifting unit 2, lifting unit 2's bottom is connected with interlude pole 3, rectangular channel has been seted up to cultivate jar 1's bottom, interlude pole 3 and rectangular channel's inner chamber interlude cooperation, interlude pole 3 is connected with cultivate jar 1's bottom interlude, lifting unit 2 includes cultivate groove 21 and sealing washer 22, cultivate groove 21 slip sets up in cultivate jar 1's inside, the outside of cultivate groove 21 is located to the fixed cover of sealing washer 22, the outer wall of sealing washer 22 is laminated with cultivate jar 1's inner wall each other, be convenient for increase the leakproofness between cultivate groove 21 and the cultivate jar 1 through sealing washer 22, be convenient for cultivate the cell through cultivate groove 21;
external threads are formed in the outer portion of the culture tank 1, a base 4 is sleeved on the external threads of the culture tank 1, the bottom end of an inserting rod 3 is connected with a mounting device 5, the mounting device 5 is connected with the base 4 in an inserting mode, a counter bore is formed in the bottom of the base 4, a through hole is formed in the top end of the counter bore, an inserting tube 51 is connected with an inner cavity of the through hole in a sliding and inserting mode, a limiting ring 52 is movably arranged in the counter bore, the mounting device 5 is conveniently hidden and contained through the counter bore, and the position of the inserting rod 3 is conveniently pushed through threaded fit between the culture tank 1 and the base 4 so as to adjust the position of the lifting assembly 2;
the mounting device 5 comprises a penetrating pipe 51, a limiting ring 52 and an elastic positioning mechanism 53, wherein the penetrating pipe 51 is connected with the base 4 in a penetrating way, the limiting ring 52 is sleeved outside the penetrating pipe 51, the elastic positioning mechanism 53 is connected with the penetrating pipe 51 in a penetrating way, the penetrating pipe 51 is cylindrical, the base 4 can rotate relative to the penetrating pipe 51, the relative position between the penetrating pipe 51 and the base 4 is limited conveniently through the cooperation between the limiting ring 52 and the elastic positioning mechanism 53, and the penetrating pipe 51 is prevented from being pulled out relative to the base 4;
the elastic positioning mechanism 53 comprises a limiting slide plate 531, a limiting clamping block 532 and a spring 533, wherein the two limiting slide plates 531 are symmetrically arranged in the penetrating pipe 51, the spring 533 is connected to the top between the two limiting slide plates 531, the two limiting clamping blocks 532 are respectively connected to one side of the two limiting slide plates 531, which is opposite to each other, the limiting clamping blocks 532 are connected with the bottom of the penetrating pipe 51 in a sliding penetrating manner, a guide rod is movably arranged in the spring 533, the limiting slide plates 531 are connected with the top of the guide rod in a sliding penetrating manner, the end portions of the guide rod are fixedly connected with the inner wall of the penetrating pipe 51 in a fixed manner, compression deformation of the spring 533 is convenient for carrying out elastic support on the two limiting slide plates 531, the penetrating between the limiting clamping blocks 532 and the penetrating pipe 51 is more stable, the limiting clamping blocks 532 are convenient for limiting the position of the limiting ring 52, the penetrating pipe 51 is prevented from moving upwards relative to the base 4, the base 4 is more stable in rotation relative to the mounting device 5, the penetrating sliding of the limiting slide plates 531 is convenient for limiting the linear sliding of the limiting slide plates 531, and the limiting slide plates 531 are more stable.
The working principle of the utility model is as follows:
in use, when a tissue sample cultured on the lifting assembly 2 needs to be observed at a short distance, only the base 4 is required to be screwed to rotate upwards, the base 4 rotates upwards relative to the culture tank 1, the mounting device 5 can neutralize the rotation motion in the screwed motion of the base 4 through the insertion between the mounting device 5 and the base 4, and the linear motion part is reserved, so that the screwed motion of the base 4 can push the position of the insertion rod 3, the insertion rod 3 pushes the lifting assembly 2 to move upwards in the culture tank 1, the lifting assembly 2 can be lifted up in the culture tank 1 for a certain height, the tissue sample cultured on the lifting assembly 2 is convenient to observe at a short distance, and the probability of dumping is reduced due to the large specific surface area of the bottom end of the base 4, so that the culture device is stable when placed on a workbench;
when need dismantle the washing to lifting unit 2, two spacing slide 531 of relative extrusion for spacing slide 531 drives two spacing fixture blocks 532 and carries out relative motion, thereby make spacing slide 531 carry out extrusion deformation to spring 533, until spacing fixture block 532 is accomodate to the inside of interlude pipe 51, spacing fixture block 532 no longer carries out spacingly to the position of spacing ring 52 this moment, spacing ring 52 drops, then alright break away from interlude with between the base 4 with penetrating pipe 51, thereby be convenient for remove the spacing between penetrating rod 3 and the base 4, then promote penetrating rod 3, thereby alright release lifting unit 2 from the inside of cultivateing jar 1, so that the washing to lifting unit 2.
Finally, it should be noted that: the foregoing description of the preferred embodiments of the present utility model is not intended to be limiting, but rather, it will be apparent to those skilled in the art that the foregoing description of the preferred embodiments of the present utility model can be modified or equivalents can be substituted for some of the features thereof, and any modification, equivalent substitution, improvement or the like that is within the spirit and principles of the present utility model should be included in the scope of the present utility model.
Claims (7)
1. A cell proliferation and differentiation culture device comprising:
the culture tank (1) is internally provided with a lifting assembly (2) in a sliding manner, the bottom end of the lifting assembly (2) is connected with a penetration rod (3), and the penetration rod (3) is connected with the bottom of the culture tank (1) in a penetration manner;
the method is characterized in that: external threads are formed on the outer portion of the culture tank (1), a base (4) is sleeved on the external threads of the culture tank (1), an installation device (5) is connected to the bottom end of the penetrating rod (3), and the installation device (5) is connected with the base (4) in a penetrating mode;
the mounting device (5) comprises a penetrating pipe (51), a limiting ring (52) and an elastic positioning mechanism (53), the penetrating pipe (51) is connected with the base (4) in a penetrating mode, the limiting ring (52) is sleeved outside the penetrating pipe (51), and the elastic positioning mechanism (53) is connected with the penetrating pipe (51) in a penetrating mode.
2. The cell proliferation and differentiation culture device according to claim 1, wherein a connection port (11) is connected to the top end of the culture tank (1), an external thread is provided on the outer wall of the connection port (11), and a sealing cover (6) is sleeved on the external thread of the connection port (11).
3. The cell proliferation and differentiation culture device according to claim 1, wherein the lifting assembly (2) comprises a culture tank (21) and a sealing ring (22), the culture tank (21) is slidably arranged inside the culture tank (1), the sealing ring (22) is fixedly sleeved outside the culture tank (21), and the outer wall of the sealing ring (22) is mutually attached to the inner wall of the culture tank (1).
4. The cell proliferation and differentiation culture device according to claim 1, wherein a rectangular groove is formed in the bottom of the culture tank (1), and the penetration rod (3) is penetrated and matched with the inner cavity of the rectangular groove.
5. The cell proliferation and differentiation culture device according to claim 1, wherein a counter bore is formed in the bottom of the base (4), a through hole is formed in the top end of the counter bore, the penetrating pipe (51) is connected with the inner cavity of the through hole in a sliding penetrating manner, and the limiting ring (52) is movably arranged in the counter bore.
6. The cell proliferation and differentiation culture apparatus according to claim 1, wherein the elastic positioning mechanism (53) comprises a limiting slide plate (531), a limiting clamping block (532) and a spring (533), the two limiting slide plates (531) are symmetrically arranged inside the penetrating pipe (51), the spring (533) is connected to the top between the two limiting slide plates (531), the two limiting clamping blocks (532) are respectively connected to one side of the two limiting slide plates (531) opposite to each other, and the limiting clamping block (532) is connected to the bottom of the penetrating pipe (51) in a sliding penetrating manner.
7. The cell proliferation and differentiation culture apparatus according to claim 6, wherein a guide rod is movably provided in the spring (533), the limit slide plate (531) is slidably inserted into the top of the guide rod, and an end of the guide rod is fixedly connected to the inner wall of the insertion tube (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321052358.XU CN219861377U (en) | 2023-05-05 | 2023-05-05 | Cell proliferation differentiation's culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321052358.XU CN219861377U (en) | 2023-05-05 | 2023-05-05 | Cell proliferation differentiation's culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219861377U true CN219861377U (en) | 2023-10-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321052358.XU Active CN219861377U (en) | 2023-05-05 | 2023-05-05 | Cell proliferation differentiation's culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219861377U (en) |
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2023
- 2023-05-05 CN CN202321052358.XU patent/CN219861377U/en active Active
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