CN219844497U - Detection cage - Google Patents
Detection cage Download PDFInfo
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- CN219844497U CN219844497U CN202320991208.9U CN202320991208U CN219844497U CN 219844497 U CN219844497 U CN 219844497U CN 202320991208 U CN202320991208 U CN 202320991208U CN 219844497 U CN219844497 U CN 219844497U
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- detection
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- cup
- urine
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- 238000001514 detection method Methods 0.000 title claims abstract description 55
- 210000002700 urine Anatomy 0.000 claims abstract description 49
- 230000000384 rearing effect Effects 0.000 claims abstract description 18
- 238000012360 testing method Methods 0.000 claims description 46
- 239000007788 liquid Substances 0.000 claims description 18
- 230000002093 peripheral effect Effects 0.000 claims description 6
- 241000699670 Mus sp. Species 0.000 abstract description 31
- 241000699666 Mus <mouse, genus> Species 0.000 description 11
- 210000003608 fece Anatomy 0.000 description 9
- 238000000034 method Methods 0.000 description 4
- 230000035935 pregnancy Effects 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000011121 vaginal smear Methods 0.000 description 2
- 241000699660 Mus musculus Species 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009894 physiological stress Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a detection cage, which comprises a feeding cage, a cage frame and a collection detection cup. The cage carries the feeder cage and forms a collecting space below the feeder cage. The collecting and detecting cup is positioned in a collecting space below the rearing cage and is used for collecting urine discharged from the rearing cage. Above-mentioned detection cage is unsettled to be placed through cage relative ground, desktop etc. placing surface with the rearing cage, and urine is concentrated through collecting the detection cup in rearing cage's below again to be used for carrying out urine detection. Therefore, enough urine can be collected only by waiting, the problems of small urine amount and difficult collection of the mice can be effectively overcome, and the difficulty of urine detection of the mice is reduced.
Description
Technical Field
The utility model relates to the technical field of detection equipment, in particular to a detection cage.
Background
The experimental mice need to be detected for conception after closing the cage, and in the related technology, the following three modes are mainly adopted: examination of thrombus, examination of sperm by vaginal smear and embryo feeling. Wherein, the examination of sperm by thrombus examination and vaginal smear can only determine whether female mice successfully mate, but can not determine whether the female mice successfully conception. The operation of fetal manipulation may lead to physiological stress in the female mice, which is prone to abortion. In addition, the gestation period of the mice is only about 21 days, so that the embryo feeling method cannot judge whether the mice are pregnant or not in the early stage of conception.
On the other hand, it is considered that the HCG hormone content in the blood of the mice increases after pregnancy, resulting in the presence of a part of HCG hormone in the urine of the mice which is excreted through the kidneys with the blood circulation system. Therefore, the level of HCG in urine of mice can be used as a criterion for judging whether the mice are pregnant.
However, given the small daily output of mouse urine relative to large animals, it is more difficult to collect alone and inconvenient to develop urine tests.
Disclosure of Invention
Based on this, it is necessary to provide a detection cage that facilitates the collection of mouse urine for detection in response to the problems described above.
A detection cage, the detection cage comprising:
a rearing cage;
a cage for carrying the rearing cage and forming a collection space below the rearing cage; and
and the collecting and detecting cup is positioned in the collecting space below the feeding cage and is used for collecting urine discharged from the feeding cage.
In one embodiment, the side wall of the collection and detection cup is provided with a slot, and the slot extends downwards from the cup opening of the collection and detection cup and is communicated with the inside of the collection and detection cup.
In one embodiment, the detection cage further comprises a net bottom, wherein the net bottom is arranged on the cage frame and is arranged at the bottom of the feeding cage in a cushioning manner.
In one embodiment, the detection cage further comprises a collection funnel disposed below the mesh floor.
In one embodiment, the cage is provided with an opening communicating with the collecting space, the bottom of the cage is configured to be open and aligned with the opening, and the mesh bottom covers the opening.
In one embodiment, the collection funnel has a maximum outer diameter that is greater than an inner diameter of the opening, and a liquid outlet of the collection funnel passes through the opening and aligns with the collection test cup within the collection space.
In one embodiment, the opening is formed in the top surface of the cage, and at least one of the peripheral side surface and the bottom surface of the cage is communicated with the collecting space and the outside.
In one embodiment, one of the feeder cage and the cage is configured with a catch and the other is configured with a bayonet with which the catch mates, the feeder cage and the cage mating through the catch and the bayonet.
In one embodiment, the collection test cup is configured as a flat bottom.
In one embodiment, the feeder cage is provided with a feeding port.
Above-mentioned detection cage is unsettled to be placed through cage relative ground, desktop etc. placing surface with the rearing cage, and urine is concentrated through collecting the detection cup in rearing cage's below again to be used for carrying out urine detection. Therefore, enough urine can be collected only by waiting, the problems of small urine amount and difficult collection of the mice can be effectively overcome, and the difficulty of urine detection of the mice is reduced.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a structure of a test cage according to an embodiment of the present utility model.
Fig. 2 is a schematic view of the structure of the feeder cage in the test cage shown in fig. 1.
Fig. 3 is a schematic structural view of the cage frame and the mesh bottom in the detection cage shown in fig. 1.
FIG. 4 is a schematic view of the structure of the collection funnel in the test cage shown in FIG. 1.
FIG. 5 is a schematic view of the structure of the collection test cup in the test cage of FIG. 1.
Fig. 6 is a top view of the collection test cup shown in fig. 5.
Reference numerals illustrate: 100. a detection cage; 10. a rearing cage; 30. a cage; 50. collecting a detection cup; 70. a net bottom; 90. a collection funnel; 91. a liquid receiving port; 93. a liquid outlet; s, collecting space; C. a slot; t, feeding port.
Detailed Description
In order that the above objects, features and advantages of the utility model will be readily understood, a more particular description of the utility model will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model. The present utility model may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the utility model, whereby the utility model is not limited to the specific embodiments disclosed below.
In the description of the present utility model, it should be understood that, if any, these terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., are used herein with respect to the orientation or positional relationship shown in the drawings, these terms refer to the orientation or positional relationship for convenience of description and simplicity of description only, and do not indicate or imply that the apparatus or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the utility model.
Furthermore, the term "and/or" is merely an association relation describing the association object, meaning that three relations may exist, e.g. a and/or B, may be represented: a exists alone, A and B exist together, and B exists alone. In addition, the character "/" herein generally indicates that the front and rear associated objects are an "or" relationship. The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present utility model, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present utility model, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Referring to fig. 1-3, one embodiment of the present utility model provides a test cage 100 comprising a feeder cage 10, a cage 30, and a collection test cup 50. The cage 30 carries the feeder cage 10 and forms a collection space S below the feeder cage 10. The collection test cup 50 is located in the collection space S below the feeder cage 10 for collecting urine discharged from the feeder cage 10.
The feeder cage 10 serves to circumscribe the range of movement of the mice, confine the mice to the feeder cage 10, and urinate within the feeder cage 10. Meanwhile, the feeding cage 10 is placed on the cage frame 30, the cage frame 30 is placed on the bearing surfaces such as the ground and the tabletop, and the feeding cage 10 is suspended in the air compared with the bearing surfaces such as the ground and the tabletop under the bearing of the cage frame 30. Below the feeder cage 10 is a collection space S.
It will be appreciated that the collection test cup 50 is placed in use in a collection space S below the feeder cage 10 for receiving urine from mice expelled within the feeder cage 10 to be used for testing.
The rearing cage 10 and the cage 30 may be made of stainless steel, and the collection and detection cup 50 may be made of plastic.
In one embodiment, the urine collected from the collection cup 50 may be used for HCG hormone testing by HCG test paper to determine if the mice in the feeder cage 10 are pregnant. In other embodiments, the collected urine from the mice may be used for other testing to determine the physiological status of the mice, which is not particularly limited herein.
The above-mentioned detection cage 100 is configured to suspend the cage 10 relative to a ground, a table surface, etc. through the cage frame 30, and collect urine under the cage 10 through the collection and detection cup 50, so as to perform urine detection. Therefore, enough urine can be collected only by waiting, the problems of small urine amount and difficult collection of the mice can be effectively overcome, and the difficulty of urine detection of the mice is reduced.
Further, one of the feeder cage 10 and the cage 30 is configured with a snap (not shown) and the other is configured with a bayonet (not shown) that mates with the snap, through which the feeder cage 10 and the cage 30 mate.
The rearing cage 10 can be fixed on the cage frame 30 through the cooperation of the buckle and the bayonet so as to improve the stability of the rearing cage, and the rearing cage 10 is difficult to push by a mouse in the moving process in the cage.
In some embodiments, the detection cage 100 further includes a mesh bottom 70, the mesh bottom 70 being disposed on the cage frame 30 and being padded to the bottom of the feeder cage 10.
The mesh bottom 70 is used to screen urine and feces, and separate feces from urine in the mouse excrement for urine collection. It will be appreciated that the mesh of the mesh substrate 70 is smaller than the diameter of the mouse faeces and that the mesh substrate 70 may be, but is not limited to, stainless steel.
The excreta from the mice falls onto the mesh floor 70 at the bottom of the feeder cage 10, wherein urine flows through the mesh openings into the collection test cup 50 and the excreta is retained on the mesh floor 70. In this way, contamination of the collection test cup 50 by fecal matter can be reduced.
Referring to fig. 4, further, the detecting cage 100 further includes a collecting funnel 90 disposed below the net bottom 70.
The collection funnel 90 is used to funnel urine from the mesh bottom 70 into the collection test cup 50. It will be appreciated that the collection funnel 90 has a liquid receiving port 91 and a liquid outlet 93, wherein the liquid receiving port 91 is aligned with the mesh floor 70 to receive urine and the liquid outlet 93 is aligned with the collection test cup 50 to output urine.
The collecting funnel 90 may be, but is not limited to, plastic, and the material is preferably smooth and hydrophobic to promote easy falling of urine under the action of gravity without urine loss caused by wall hanging and bead hanging.
The collection funnel 90 can increase the concentration of urine and deliver the concentrated urine to the collection test cup 50. The diameter of the collection test cup 50 need not cover the entire bottom of the feeder cage 10, but need only be capable of receiving the outlet 93 of the collection funnel 90.
In some embodiments, the cage 30 is provided with an opening communicating with the collection space S, and the bottom of the cage 30 is configured to be open and aligned with the opening, and the mesh bottom 70 is disposed over the opening.
At this time, the net bottom 70 also has a function of supporting the movement of the mice in the rearing cage 10, and excreta generated by the mice are directly discharged to the net bottom 70 for screening. Wherein urine flows through the mesh floor 70 into the collection funnel 90 through the openings and is returned to the collection test cup 50.
In other embodiments, the bottom of the feeder cage 10 may also be configured with a cage bottom, and the cage bottom should be a structure such as a mesh that is capable of at least allowing urine to penetrate downward.
Further, the largest outer diameter of the collection funnel 90 is larger than the inner diameter of the opening, and the liquid outlet 93 of the collection funnel 90 passes through the opening and is aligned with the collection detection cup 50 in the collection space S.
The maximum outer diameter of the collection funnel 90 is larger than the inner diameter of the opening, meaning that the collection funnel 90 does not pass completely through the opening after the outlet 93 passes through the opening, but is stuck in the opening of the cage 30, thus achieving its own fixation.
Generally, the largest outer diameter of the collection funnel 90, i.e., the outer diameter of the liquid receiving opening 91 thereof, is preferably slightly larger than the inner diameter of the opening of the collection funnel 90. The outer diameter of the mesh bottom 70 may be substantially the same as the outer diameter of the liquid receiving port 91, and the mesh bottom 70 may be disposed on the liquid receiving port 91 of the collection funnel 90 after the collection funnel 90 is mounted on the cage 30 by being caught at the opening.
Specifically, the bottom outer edge of the feeder cage 10 is configured with a snap, the cage 30 is configured with a bayonet, and the bayonet of the cage 30 is configured at the opening edge. In this way, the feeder cage 10 is positioned with the bottom open towards the opening of the cage 30 after mounting by snap-fit with the bayonet fitting of the cage 30.
In one embodiment, cage 30 is square, having a size of 20cm by 20cm, an inner diameter of the upper opening of 15cm, and a diameter of mesh floor 70 of greater than 15cm. The inner diameter of the liquid receiving port 91 of the collecting funnel 90 is 15cm, and correspondingly, the outer diameter thereof is slightly larger than 15cm, and the inner diameter of the liquid outlet 93 of the collecting funnel 90 is 0.5cm. The collection test cup 50 has an inner diameter of 1cm and receives the liquid outlet 93 of the collection funnel 90 when collecting urine.
In some embodiments, the opening is opened on the top surface of the cage 30, and at least one of the peripheral side surface and the bottom surface of the cage 30 communicates with the collection space S and the outside.
The collection space S communicates with the outside through the peripheral side surface and/or the bottom surface of the cage 30, and the collection test cup 50 can enter the collection space S from the peripheral side surface or the bottom surface. Wherein, when the peripheral side surface communicates the collecting space S with the outside, the operation of the collecting and detecting cup 50 in the collecting space S can also be facilitated.
Preferably, the cage 30 is square in shape and is hollow on all six sides except the top side. Cage 30 may be considered to be comprised of a plurality of prisms and a top surface.
In some embodiments, feeder cage 10 is provided with a feeding port T. Wherein, the feeding port T is at least one.
Specifically, there may be two feeding ports T, one of which may be used for placement of a feed cartridge and the other for access to the mouthpiece of the drinking bottle.
The time that the mice remain in the test cage 100 may be longer in order to collect sufficient urine. The feeding port T may facilitate feeding food and water to the mice in the feeder cage 10 to better maintain their normal lives and also facilitate urination by the mice.
Referring to fig. 5 and 6, in some embodiments, a slot C is formed on a side wall of the collection and detection cup 50, and the slot C extends downward from a cup opening of the collection and detection cup 50 and is communicated with the inside of the collection and detection cup 50.
In other words, the slot C extends downwards from the top edge of the cup mouth of the collecting and detecting cup 50, the slot C is communicated with the inside of the cup, and urine collected in the cup can enter the slot C.
The slot C is used for inserting detection test paper, and the test paper can be fixed by the slot C and is soaked by urine in the slot C for detection.
Preferably, the bottom of the slot C communicates with the bottom of the collection test cup 50, and the slot C and the space in the cup form a communicating vessel communicating from the respective bottoms.
The difficulty of entering urine infiltration test paper in the slot C can be reduced through bottom communication, and the completion of detection is facilitated.
Still further, the collection test cup 50 is configured as a flat bottom.
The flat bottom helps the collection test cup 50 to be placed smoothly on a floor, table top, or the like.
In use of the test cage 100, the collection test cup 50 is placed in the collection space S of the cage 30, and the liquid outlet 93 of the collection funnel 90 is inserted into the collection test cup 50 through the opening of the cage 30. The funnel 90 to be collected is moved to be caught by the opening, and a net bottom 70 is placed toward the liquid receiving port 91 of the funnel 90. Finally, the snap of the feeder cage 10 is snapped into alignment with the snap of the cage 30 such that the feeder cage 10, along with the mice therein, is placed on the cage 30.
The mice were housed in the test cages 100 within 3 to 5 days after mating, and HCG test strips were inserted into the slots C of the collection test cups 50. Only need to wait and give sufficient water and food or feed water in good time. After the mice excrete, the excreta is screened by the mesh bottom 70, the feces are trapped on the mesh bottom 70, and urine flows through the mesh bottom 70 to the collection funnel 90 below the mesh bottom 70, and is collected by the collection funnel 90 into the collection test cup 50.
The urine liquid level can react after contacting with HCG test paper in the slot C, if the mouse is pregnant, the HCG test paper can display the concentration of the urine HCG, if the color development concentration of the test paper exceeds the judgment critical value by 10mIU/ml, the pregnancy of the mouse can be primarily judged, and if the two continuous tests show that the concentration is higher than the judgment critical value, the pregnancy of the mouse can be judged.
Urine is collected by the detection cage 100, and HCG detection is performed by using the urine, so that the problem that whether a mouse is pregnant or not cannot be judged in a short period after mating is solved, and the mouse to be detected is only required to be placed into the detection cage 100 in the afternoon every day. The detection result of the HCG detection test paper can be observed the next day, the difficulty in the animal experiment process related to the current mouse breeding or reproduction field is effectively solved, the experiment efficiency and the accuracy are improved, and the method has remarkable economic value in the aspect of breeding and seed conservation of transgenic mice.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of protection of the present utility model is to be determined by the appended claims.
Claims (9)
1. A test cage, the test cage comprising:
a rearing cage (10);
a cage (30) carrying the feeder cage (10) and forming a collection space (S) below the feeder cage (10); and
a collection and detection cup (50) positioned in the collection space (S) below the rearing cage (10) and used for collecting urine discharged from the rearing cage (10); the side wall of the collecting and detecting cup (50) is provided with a slot (C), and the slot (C) extends downwards from the cup opening of the collecting and detecting cup (50) and is communicated with the inside of the collecting and detecting cup (50).
2. The detection cage according to claim 1, characterized in that it further comprises a mesh bottom (70), said mesh bottom (70) being provided on said cage (30) and being padded to the bottom of said feeder cage (10).
3. The detection cage according to claim 2, characterized in that it further comprises a collection funnel (90) arranged below the mesh bottom (70).
4. A detection cage according to claim 3, characterized in that the cage (30) is provided with an opening communicating with the collecting space (S), the bottom of the cage (30) being configured to be open and aligned with the opening, the mesh bottom (70) being provided over the opening.
5. The detection cage according to claim 4, characterized in that the largest outer diameter of the collection funnel (90) is larger than the inner diameter of the opening, from which opening the liquid outlet (93) of the collection funnel (90) passes and is aligned with the collection detection cup (50) in the collection space (S).
6. The detection cage according to claim 4, characterized in that the opening is provided in the top surface of the cage (30), at least one of the peripheral side surface and the bottom surface of the cage (30) communicating the collecting space (S) with the outside.
7. The detection cage according to claim 1, characterized in that one of the feeder cage (10) and the cage (30) is configured with a catch and the other is configured with a bayonet with which the catch cooperates, the feeder cage (10) and the cage (30) being engaged by the catch and the bayonet.
8. The detection cage according to claim 1, characterized in that the collection detection cup (50) is configured as a flat bottom.
9. The detection cage according to claim 1, characterized in that the feeder cage (10) is provided with a feeding opening (T).
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