CN219593503U

CN219593503U - Low-temperature mechanical perfusion preservation device

Info

Publication number: CN219593503U
Application number: CN202320512996.9U
Authority: CN
Inventors: 罗令
Original assignee: Shanghai Inzex Biotechnology Co ltd; Shanghai Jianfeng Medical Science And Technology Co ltd
Current assignee: Shanghai Inzex Biotechnology Co ltd; Shanghai Jianfeng Medical Science And Technology Co ltd
Priority date: 2022-04-28
Filing date: 2023-03-16
Publication date: 2023-08-29
Anticipated expiration: 2033-03-16
Also published as: CN114766470A

Abstract

The utility model provides a low-temperature mechanical perfusion preservation device, which comprises an organ box for placing an organ to be transplanted, a coolant container, a filter, a bubble catcher, a semiconductor refrigeration element, a pump, a perfusion circuit, a circulation circuit, a sensor component, an electromagnetic valve component and a control display unit, wherein an opening is arranged on the side edge of the coolant container, and a temperature sensor detects the temperature of perfusate in the organ box through the opening; a partition board is erected at the bottom of the bubble catcher and divides the bubble catcher into a plurality of communicated chambers; the semiconductor refrigeration element is assembled outside the bubble trap. The low-temperature mechanical perfusion preservation device can directly detect the temperature of the perfusion liquid, can cool the perfusion liquid with the temperature exceeding the standard to a set temperature range as soon as possible, can prevent bubbles from entering a perfusion pipeline as far as possible, and improves the accuracy of perfusion data and the perfusion safety.

Description

Low-temperature mechanical perfusion preservation device

Technical Field

The utility model belongs to the field of biomedical instruments, and particularly relates to a low-temperature mechanical perfusion preservation device for organ transplantation.

Background

Organ transplantation refers to the surgical transfer of viable ex vivo organs into a patient. The temperature must be controlled in the low temperature environment of 2-8deg.C during organ storage, and the traditional organ preservation method is static cold preservation. The longer the static cold preservation time, the higher the incidence of delayed recovery of graft function after surgery, and in addition, the static cold preservation mode is difficult to objectively evaluate the isolated organ. Compared with static cold preservation, the low-temperature mechanical perfusion of the isolated organ can obviously reduce the occurrence rate of delayed recovery of the function of the graft, is beneficial to improving the long-term survival rate of the transplanted organ, and meanwhile, the mechanical perfusion parameters can assist in evaluating the quality of the transplanted organ, thereby being beneficial to the full utilization of the isolated organ. The organ perfusion transfer box is an in-vitro organ transfer, preservation and low-temperature mechanical perfusion device for transplantation, can prolong the storage time of the in-vitro organ, evaluate the quality of the in-vitro organ and reduce the incidence rate of DGF (delayed recovery of transplanted organ function) after operation, and is widely applied. For example, the life port kidney perfusion kit proposed by LSI in the united states is the most widely used cold mechanical perfusion type product in clinic, and has been registered and sold in three markets, china, the united states and europe.

Typically, the perfusate needs to be cooled to a suitable low temperature prior to perfusion. Taking a life port kidney perfusion operation box as an example, the perfusion temperature safety of the life port kidney perfusion operation box is mainly realized by controlling the temperature of the ice box. The ice box is provided with a thermal resistance type sensor for detecting the temperature of the ice box, a temperature range of 0-8 ℃ is set for the temperature of the ice box by a control system, and when the temperature of the ice box is lower than 0 ℃ or higher than 8 ℃, the equipment cannot normally operate. However, the above-mentioned perfusate temperature control method has a certain risk, since the temperature of the ice box is detected instead of directly detecting the temperature of the perfusate, if the perfusate which is not cooled in place is put into the perfusion apparatus with the ice box, the isolated organ is perfused for about half an hour or more in a state of being higher than the ideal temperature, and then the perfusate can be cooled to a proper temperature by the ice box, which may cause irreversible damage to the isolated organ.

The lack of pre-cooling of the perfusate may also be related to limited preservation conditions after removal of the refrigerator, and when the perfusate temperature in the operation box device is too high, the device is too low in cooling efficiency by means of the physical ice box, because the physical ice box has the main function of maintaining the perfusate temperature, and a doctor is required to wait for the perfusate temperature to be lower than 8 ℃ and then install the isolated organ and perform perfusion, the waiting time may exceed 1 hour, and obviously, such long waiting time is not available in the operation process, and the existing operation box device lacks a mechanism for rapid cooling.

The organ perfusion transfer box maintains a low temperature environment of the perfusate by means of a physical ice box filled with 5.5kg of ice-water mixture, so that at least 4.5kg of crushed ice and 1kg of ice water must be prepared for filling the physical ice box before use, and when several kidneys are simultaneously perfused, much time is required for preparing enough crushed ice and ice water, and filling is required one by one before perfusion, which is a complicated process.

The organ perfusion transfer box has the effect of assessing the quality of the isolated organ by assessing the quality of the isolated kidney through perfusion resistance, changes in flow parameters and final results, so the accuracy of perfusion flow and resistance often affects clinical assessments. The organ perfusion transfer box obtains flow parameters by calculating the rotating speed of the peristaltic pump per minute, and the resistance is calculated by the detection pressure and the flow of the pressure sensor. When the peristaltic pump is abnormal, if the fixer is loose and the pump pipe falls off, the fixed flow pumped by the peristaltic pump in a single cycle can be changed (for example, the fixed flow is reduced), in order to achieve the set pressure, the peristaltic pump must increase the rotating speed, a virtual high false flow can be formed under the same set pressure, the false flow is substituted into the calculation to form a virtual low false resistance, and because the existing operation box equipment only calculates the flow by means of the rotating speed of the peristaltic pump, the accuracy of the flow cannot be identified, an inaccurate flow prompt cannot be sent, and the false flow and the resistance can often misguide clinical judgment.

When perfusate enters an isolated organ, entrainment of air bubbles into the organ should be avoided, and therefore it is often desirable to have the perfusate pass through the bubble trap in advance to remove the air bubbles. The bubble trap can discharge bubbles in the pipeline through a circulation port at the top end. During circulation, the circulation port is opened to purge air or other gases from the perfusate path. From the current practice of application of various cryogenic mechanical perfusion-type products, there is still a risk that the bubbles in the bubble trap continue to flow downstream and into the organ.

In general, the existing organ perfusion transfer box still has room for improvement in terms of convenience in use, perfusion safety, data accuracy and the like.

Disclosure of Invention

The utility model provides a low-temperature mechanical perfusion preservation device, which improves the accuracy of perfusion temperature detection and the perfusion safety.

The technical scheme adopted by the utility model is as follows:

a low-temperature mechanical perfusion preservation device comprises an organ box for placing an organ to be transplanted, a coolant container, a pump, a filter, a bubble catcher, a sensor assembly, an electromagnetic valve assembly and a control display unit, wherein the sensor assembly comprises a bubble sensor, a pressure sensor, a flow sensor and a temperature sensor;

the coolant container surrounds the periphery of the organ box, an opening is arranged on the side edge of the coolant container, and a temperature sensor for detecting perfusate in the organ box is arranged on the outer side surface of the opening;

the bubble catcher comprises three ports, namely a liquid inlet, a pouring outlet and a circulating outlet, wherein a baffle is vertically arranged at the bottom of the bubble catcher, and divides the bubble catcher into a plurality of communicated chambers;

a semiconductor refrigerating element is assembled outside the bubble catcher;

the filling loop comprises a pump, a filter, a bubble catcher, a main pipeline and a filling pipeline, wherein the pump, the filter and the bubble catcher are sequentially connected through the main pipeline, a filling outlet of the bubble catcher is connected with the filling pipeline, and a bubble sensor, a pressure sensor and a flow sensor are arranged on the filling pipeline;

the circulating loop comprises a pump, a filter, a bubble catcher, a main pipeline and a circulating pipeline, wherein the pump, the filter and the bubble catcher are sequentially connected through the main pipeline, and a circulating outlet of the bubble catcher is connected with the circulating pipeline.

Further, the filling pipeline is a Y-shaped pipeline and comprises a main filling pipeline, a filling branch and a circulating branch, and the circulating branch is communicated with the circulating pipeline through an interface.

Further, the temperature sensor is an infrared temperature sensor, the bubble sensor is an ultrasonic sensor, and the pressure sensor is a gas pressure sensor.

Further, the height of the partition plate accounts for 1/3-1/2 of the total height of the bubble trap.

Further, the semiconductor refrigeration element is a peltier refrigeration plate and is disposed on one side of the bubble trap.

Further, the system comprises an internet of things module, and is used for uploading the perfusion real-time data to a cloud or mobile terminal.

Further, an oxygenator is provided between the filter and the bubble trap to supply oxygen to the liquid path.

Compared with the prior art, the utility model has the following advantages:

1. the temperature of the perfusate is directly detected by arranging an infrared temperature sensor on the ice box near the perfusate, so that the perfusate with proper temperature is ensured to enter the organ; meanwhile, the semiconductor refrigerating element is arranged on the side face of the bubble catcher, so that the perfusate with the temperature exceeding the standard is cooled to the set temperature range as soon as possible, and the temperature safety of the perfusate is ensured;

2. the separator is arranged in the bubble catcher, so that bubbles can be prevented from entering the filling pipeline as far as possible, and the filling safety is ensured; meanwhile, only one bubble sensor is needed to be arranged on the whole filling pipeline, so that parts are reduced;

3. the pressure sensor and the flow sensor are arranged on the perfusion pipeline, so that the stability of perfusion can be monitored in real time, and equipment faults can be found in time;

4. the Internet of things function is achieved.

Drawings

FIG. 1 is a schematic diagram of a perfusion apparatus according to a first embodiment of the present utility model;

FIG. 2 is a cross-sectional perspective view of a bubble trap of the perfusion apparatus according to the first embodiment of the present utility model;

FIG. 3 is a schematic diagram of a perfusion apparatus according to a second embodiment of the present utility model;

FIG. 4 is a cross-sectional view of an oxygenator of a perfusion apparatus according to a second embodiment of the present utility model;

in the figure, 1-ice bin; 2-a pump; 3. 4, 5-electromagnetic valve; 6-controlling the display unit; 8-1, 8-2, 8-3-main pipeline; 9-semiconductor refrigeration element; a 10-20 μm filter; 11-a bubble trap; 12-a circulation pipeline; 13-pouring a main pipeline; 14-priming a branch; 15-a circulation branch; 16-bubble sensor; 17-a pressure sensor; 18-a flow sensor; a 19-oxygenator; a 19-1-oxygenator air inlet, a 19-2-oxygenator liquid inlet, a 19-3-oxygenator liquid outlet, a 19-4-oxygenator sampling port and a 20-oxygen bottle.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments of the present utility model, but the embodiments described below are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Fig. 1-3 show an embodiment of a cryogenic mechanical perfusion preservation device, mainly comprising an organ box (not shown) for placing an organ to be transplanted, an ice box 1, a pump 2, a filter 10, a bubble trap 11, a semiconductor refrigeration element 9, a perfusion circuit, a circulation circuit, a sensor assembly, solenoid valve assemblies 3, 4, 5, a control display unit 6, wherein the sensor assembly comprises a bubble sensor 16, a pressure sensor 17, a flow sensor 18, a temperature sensor (not shown). The whole device is insulated from the external environment.

The control display unit 6 comprises an external instruction input module, a data storage and analysis module, a controller and display module, a warning unit and an internet of things module.

The external command input module may provide inputs for power switches, pressure adjustments, priming modes, cycling modes, etc. The display module may display perfusion parameters (including pressure, flow, temperature, perfusion time, etc.), organ information, alarm information, etc. The external command input module and the display module may be integrated on one touch display screen.

The data storage and analysis module compares the detection value of the sensor with a preset value range and feeds back the comparison result to the controller. The controller can respond to the comparison result of the detection values of the temperature sensor to select a filling operation or a circulating operation or control the starting and stopping of the semiconductor refrigeration element; a priming operation or a venting operation may be selected in response to the value of the bubble sensor; the leakage or pump abnormality can be prompted in the display unit based on the flow sensor and the flow value of the pump; the output flow of the pump may be controlled in response to the value of the hydraulic line pressure sensor.

The organ box may have a cradle on which the organ is disposed, and the organ may be an isolated kidney of a human or animal. The organ box and/or tray are configured to allow a perfusate bath to form around the organ. The organ box is configured to provide uninterrupted sterile conditions during shipping, recovery, analysis, and storage while maintaining thermal conduction with the ice box.

The organ box is arranged in an ice box 1 with a recess, in which a cooling medium such as ice, ice water, saline water or the like can be accommodated, and any other suitable cooling medium, such as a polymer phase change gel material, can be used. In use, the organ is disposed within the cradle, the cradle is disposed within the organ box, and the organ box is disposed within the ice box such that the ice box surrounds the periphery of the organ box such that the organ box is maintained at a low temperature environment of 0-8 ℃, the ice box providing cooling to the organ without directly contacting the organ.

A hole (not shown) with a diameter of 30mm is formed at a position 100mm above the side surface of the ice box 1, an infrared temperature sensor is arranged on the outer side wall near the hole, and the temperature of perfusate in the organ box in the ice box can be directly detected through the hole, so that the practicability and accuracy of the temperature sensor are improved. The detected temperature is designated as T1, the temperature range of T1 is 0-7 ℃, and when T1 is lower than 0 ℃ or higher than 7 ℃, the device cannot perform perfusion operation and only performs circulation operation.

The filter 10 serves to filter particulate matter in the fluid path, preventing particulate matter from entering and clogging the flow path of the device. The particulate matter may be, for example, fat particles or blood clots produced by an isolated organ. The filter may be a single filter or a combination of filters of different pore sizes, here 1 20 μm filter purchased from outsource.

The pump 2 may be any pump suitable for use in combination with perfusion of an organ. Examples of suitable pumps may include manually operated pumps, centrifugal pumps, and peristaltic pumps, here employing commercially available 75 speed DC motor peristaltic pumps.

The bubble trap 11 preferably separates bubbles that may be entrained in the perfusate flow, preventing these bubbles from continuing downstream and into the extracorporeal organ. The bubble trap can also be used as a heat exchange unit for rapid refrigeration of perfusate. The bubble trap is generally square at the lower part and triangular at the upper part, and includes a circulation outlet that allows venting during circulation or priming. The circulation outlet may be connected to the perfusion flow path. After the circulation mode is started, the circulation line connected to the circulation outlet is opened to purge air or other gas. Once the gas is purged from the perfusate path, the circulation outlet may be closed. The circulation outlet may be closed by a controller in the control and display unit controlling a solenoid valve.

In the embodiment, the design capacity of the bubble trap is 300ml, the port A of the bubble trap is a liquid inlet, the port B is a pouring outlet, a baffle plate is vertically arranged at the middle position of the bottom of the bubble trap, and the height of the baffle plate accounts for 1/3-1/2 of the total height of the bubble trap. The perfusate enters the left side of the cavity of the bubble catcher, the water level reaches the top end of the baffle plate and then flows into the right side of the cavity, and bubbles in the perfusate move upwards along the baffle plate and are collected to the top end of the cavity, namely the circulating outlet C, due to the arrangement of the baffle plate. In the circulation mode, the opening A and the opening C are in an open state, the opening B is in a closed state, the perfusate enters from the opening A, flows out from the opening C, and bubbles in the pipeline are taken away. Under the filling mode, the C mouth is closed, the A mouth and the B mouth are open, the filling liquid enters from the A mouth, and flows out from the B mouth, the bubbles are converged to the C mouth, the device is switched from the filling mode to the circulating mode every 10 minutes, the C mouth is opened, and the bubbles are discharged from the C mouth along with the filling liquid. A bubble sensor 16 is also provided around the perfusion line downstream of the bubble trap for detecting if bubbles remain in the perfusate. If the presence of a bubble is detected, the priming mode is stopped and the venting mode is switched. The bubble sensor may be an ultrasonic bubble sensor which may not contact the perfusate and thus need not be clean and easy to replace.

The bubble trap is internally provided with a temperature sensor which is positioned on the narrow side (not shown), the temperature of the perfusate in the bubble trap is detected, the temperature range of T2 is marked as T2, the temperature range of T2 is 0-8 ℃, and when the temperature of T2 is lower than 0 ℃ or higher than 8 ℃, the device can not perform perfusion operation. The bubble trap is laterally fitted with a semiconductor refrigerating element 9, which may be a peltier refrigerating plate, which improves the waiting time when the temperature of the perfusate in the pouring box is higher than 8 c. When the temperature sensor senses that the temperature of the perfusate is higher than 8 ℃ and is in a power line power supply mode, the equipment prompts to enter a circulation mode, liquid starts to circulate rapidly, meanwhile, the controller controls the semiconductor refrigeration element to start, and the refrigeration sheet is attached to the bubble catcher to output low temperature, so that the perfusate is reduced below 8 ℃ as soon as possible.

The perfusion pipeline is provided with a flow sensor for detecting the flow of perfusion liquid flowing through the pipeline, and the flow sensor can be an ultrasonic flow sensor. The flow sensor is arranged in the pipeline, and by comparing the detection data of the flow sensor with the flow data of the peristaltic pump, whether the peristaltic pump fails or not and whether the filling system leaks or not can be timely judged, so that the problem of inaccurate display flow caused by deformation, aging or failure of the peristaltic pump assembly due to the fact that the peristaltic pump is only dependent on the flow data of the peristaltic pump can be solved, and the accuracy of the filling data is improved.

The perfusion pipeline is provided with a liquid path pressure sensor 17. The hydraulic circuit pressure sensor detects the real-time pressure of the hydraulic circuit and provides overpressure monitoring. In the event that the pressure of the perfusion fluid flowing through the tubing exceeds a predetermined threshold, the device can automatically stop and/or reduce the flow provided by the pump to prevent damage to the organ.

The perfusion circuit comprises peristaltic pump 2, main lines 8-1, 8-2 and 8-3, filter 10, bubble trap 11, Y-shaped perfusion lines including perfusion main line 13, perfusion branch 14 and circulation branch 15, which may be suitable flexible fluid conduits. The perfusate inlet of the organ box is connected with one end of a peristaltic pump through a main pipeline 8-1, the other end of the peristaltic pump is connected with the liquid inlet of a filter 10 through a main pipeline 8-2, the liquid outlet of the filter 10 is connected with the liquid inlet of a bubble catcher 11 through a main pipeline 8-3, the liquid outlet of the bubble catcher 11 is connected with a perfusing main pipeline 13 and a perfusing branch 14, and the liquid outlet end of the perfusing branch 14 stretches into the lower part of the perfusate liquid level of the organ box to form a perfusing loop. Wherein, be provided with bubble sensor 16 on the main line 13 of pouring, be provided with flow sensor 18 and liquid way pressure sensor 17 on the branch line 14 of pouring, circulation branch line 15 communicates with circulation line 12. The isolated organ is soaked in the low-temperature perfusate in the organ box, and the peristaltic pump in the device can provide power to enable the low-temperature perfusate to enter from the artery and flow out from the vein of the organ and form circulation, so that the low-temperature mechanical continuous perfusion of the isolated organ is realized.

The circulation loop comprises peristaltic pump 2, main lines 8-1, 8-2 and 8-3, filter 10, bubble trap 11 and circulation line 12. The perfusate inlet of the organ box is connected with one end of a peristaltic pump 2 through a main pipeline 8-1, the other end of the peristaltic pump is connected with the liquid inlet of a filter 10 through the main pipeline 8-2, the liquid outlet of the filter 10 is connected with the liquid inlet of a bubble catcher 11 through a main pipeline 8-3, the liquid outlet of the bubble catcher 11 is connected with one end of a circulating pipeline 12, and the other end of the circulating pipeline 12 stretches into the position below the perfusate liquid level of the organ box to form a circulating loop. The circulation line 12 is provided with an interface (not shown), and the circulation branch 15 communicates with the circulation line 12 through the interface.

1. Circulation mode

In the circulation mode, the controller controls the solenoid valve 3 to open and the solenoid valves 4 and 5 to close. Peristaltic pump 2 operates to cause perfusate to flow through circulation lines 8-1 and 8-2, filter 10, main line 8-3, bubble trap 11, and circulation line 12 in that order, removing bubbles.

2. Perfusion mode

In the perfusion mode, the controller controls the solenoid valve 5 to open, the solenoid valves 3 and 4 to close, and the peristaltic pump 2 to operate so that perfusate flows through the main lines 8-1 and 8-2, the filter 10, the main line 8-3, the bubble trap 11, the perfusion main line 13 and the perfusion branch 14 in sequence.

When the bubble sensor 16 on the main filling pipe 13 detects that bubbles exist in the filling liquid flowing through the main filling pipe 13, the controller controls the electromagnetic valve 5 to be closed, the electromagnetic valve 4 to be opened, the filling liquid is discharged through the circulation branch 15 and the circulation pipe 12, the exhaust mode is started until the bubble sensor no longer detects the bubbles, the controller controls the electromagnetic valve 5 to be opened, the electromagnetic valve 4 to be closed, and the filling mode is restarted.

The flow sensor 18 on the perfusion branch 14 monitors the flow B of the perfusion fluid flowing through the perfusion branch 14, meanwhile, the peristaltic pump 2 can monitor the pipeline pumping flow A, when the flow B is smaller than a certain value A, the leakage of the middle section of the pipeline or the abnormal pumping of the peristaltic pump is deduced, the controller controls the perfusion stopping mode, and meanwhile, the device gives a warning.

The fluid pressure sensor 17 on the perfusion branch 14 monitors the pressure of the perfusate flowing through the perfusion branch 14 and when the pressure exceeds a predetermined threshold, the controller controls the peristaltic pump to reduce the flow supply.

The following describes the operation of the cryogenic mechanical perfusion preservation device, including the steps of:

step one, installing a perfusion circuit and a circulation circuit, filling perfusion liquid, and starting up;

setting the pouring parameters such as pressure, flow, temperature, pouring time and the like through a control display unit on the equipment, starting a circulation mode, and operating a peristaltic pump to circulate the pouring liquid in a main pipeline, a filter, a bubble catcher and a circulation pipeline;

thirdly, after the circulation mode is finished, connecting the isolated organ into an organ box by using a T-shaped connecting sleeve, wherein two ends of the T-shaped connecting sleeve are respectively connected with an organ artery and a perfusion branch, opening the connecting sleeve to be not connected with a tail end cap, starting an exhaust mode, operating a peristaltic pump, enabling perfusion liquid to flow out from the unconnected tail end of the connecting sleeve through a main pipeline, a filter, a bubble catcher, a perfusion main pipeline and the perfusion branch, and discharging bubbles of the perfusion pipeline;

step four, after the exhaust mode is finished, covering a cap of the unconnected end of the connecting sleeve, and detecting the tightness of the liquid path;

step five, starting a perfusion mode, and running a peristaltic pump to enable perfusion liquid to enter an isolated organ artery through a main pipeline, a filter, a bubble catcher, a perfusion main pipeline and a perfusion branch; when a bubble sensor on the perfusion main pipeline detects that bubbles exist in the perfusion liquid, stopping the perfusion mode, discharging the perfusion liquid through the circulation branch until the bubble sensor does not detect the bubbles any more, and restarting the perfusion mode; when the difference between the flow monitored by the flow sensor on the filling branch and the pumping flow of the peristaltic pump exceeds a set interval, stopping the filling mode and simultaneously giving a warning; the controller controls the peristaltic pump to reduce the flow supply when the pressure monitored by the fluid circuit pressure sensor on the perfusion circuit exceeds a predetermined threshold.

Fig. 4 shows another embodiment of the cryogenic mechanical perfusion preservation device, differing from the first embodiment in that an oxygenator 19 is added in the flow path, the oxygenator being arranged between the filter and the bubble trap. The oxygenator 19 is provided with an oxygen inlet 19-1, a perfusate inlet 19-2, a perfusate outlet 19-3 and a sampling port 19-4. The oxygen source can be high-pressure oxygen in a hospital, a large-sized oxygen bottle or a customized small-sized oxygen bottle, wherein the oxygen bottle 20 is adopted, and meanwhile, a gas flowmeter is arranged, so that the oxygen flow is accurately controlled.

Oxygen enters the perfusate through the inlet 19-1 of the oxygenator, increasing the oxygen content of the perfusate. Perfusate can be periodically withdrawn from sampling port 19-4 and the oxygen content therein detected, and when the oxygen content is too low, the controller controls the gas flow meter to increase the oxygen flow.

Claims

1. A cryogenic mechanical perfusion preservation device, which is characterized by comprising an organ box for placing an organ to be transplanted, a coolant container, a pump, a filter, a bubble catcher, a sensor assembly, a solenoid valve assembly and a control display unit, wherein the sensor assembly comprises a bubble sensor, a pressure sensor, a flow sensor and a temperature sensor;

the coolant container is arranged around the periphery of the organ box, an opening is arranged on the side edge of the coolant container, and the outer side surface of the opening is provided with the temperature sensor for detecting perfusate in the organ box;

the bubble catcher comprises three ports, namely a liquid inlet, a pouring outlet and a circulating outlet, wherein a baffle is vertically arranged at the bottom of the bubble catcher, the baffle divides the bubble catcher into a plurality of communicated chambers, and the height of the baffle accounts for 1/3-1/2 of the total height of the bubble catcher;

a semiconductor refrigeration element is assembled outside the bubble catcher, and the semiconductor refrigeration element is a Peltier refrigeration sheet and is arranged on one side surface of the bubble catcher;

the filling loop comprises a pump, a filter, a main pipeline and a filling pipeline, wherein the pump, the filter and the bubble catcher are sequentially connected through the main pipeline, a filling outlet of the bubble catcher is connected with the filling pipeline, and the filling pipeline is provided with the bubble sensor, the pressure sensor and the flow sensor;

the circulation loop comprises the pump, the filter, the bubble catcher, the main pipeline and the circulation pipeline, wherein the pump, the filter and the bubble catcher are sequentially connected through the main pipeline, and the circulation outlet of the bubble catcher is connected with the circulation pipeline.

2. The cryogenic mechanical perfusion preservation device of claim 1, wherein the perfusion pipeline is a Y-type perfusion pipeline, comprising a perfusion main pipeline, a perfusion branch and a circulation branch, the circulation branch being in communication with the circulation pipeline through an interface of the circulation pipeline.

3. The device of claim 1, wherein the temperature sensor is an infrared temperature sensor, the bubble sensor is an ultrasonic sensor, and the pressure sensor is a gas pressure sensor.

4. The device of claim 1, further comprising an internet of things module configured to upload the perfusion real-time data to a cloud or mobile terminal.

5. The cryogenic mechanical perfusion preservation apparatus of claim 1 further comprising an oxygenator disposed between the filter and the bubble trap.