CN219578160U - Stem cell low-temperature preservation device - Google Patents
Stem cell low-temperature preservation device Download PDFInfo
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- CN219578160U CN219578160U CN202321369675.4U CN202321369675U CN219578160U CN 219578160 U CN219578160 U CN 219578160U CN 202321369675 U CN202321369675 U CN 202321369675U CN 219578160 U CN219578160 U CN 219578160U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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Abstract
The utility model discloses a low-temperature stem cell preservation device, which belongs to the technical field of stem cell preservation, and adopts the technical scheme that the low-temperature stem cell preservation device comprises a placement disc and a fixed disc positioned above the placement disc, wherein the upper end surface of the placement disc is provided with a plurality of evenly distributed placement grooves, the upper end surface of the fixed disc is provided with a plurality of fixing holes matched with the placement grooves in a penetrating way, the inside of the placement groove is provided with two fixing plates which are oppositely arranged, springs are arranged between one ends of the two opposite fixing plates and the placement grooves, the low-temperature preservation of stem cell test tubes in the preservation device can be realized through a cooling cavity, a plurality of test tubes with stem cells are respectively placed in the placement grooves, the fixing plates are extruded according to the size of the test tubes, the springs are extruded by the fixing plates, the springs are forced to be compressed until the stem cell test tubes are completely placed in the placement grooves, and the upper sides of the stem cell test tubes are positioned in the fixing holes, and the stable placement of the stem cell test tubes can be realized through the placement disc and the fixing plates.
Description
Technical Field
The utility model relates to the technical field of stem cell preservation, in particular to a low-temperature stem cell preservation device.
Background
Stem cells are a type of multipotent cells with self-renewal capacity, which can differentiate into a variety of multipotent cells under certain conditions, and are a type of primitive cells with self-replication and multipotent differentiation potential, i.e., stem cells remain in an undirected differentiated state and have proliferation capacity, and can differentiate into a variety of functional cells or tissue organs under appropriate conditions or given appropriate signals, and are classified into embryonic stem cells and adult stem cells according to the developmental stage in which the stem cells are located. Three categories are distinguished by the developmental potential of stem cells: totipotent stem cells, pluripotent stem cells, and monopotent stem cells. Stem cells are an insufficiently differentiated, yet immature cell with the potential function of regenerating various tissues and organs and the human body, and are called "universal cells" in the medical community. Stem cells require a low temperature environment for storage. A currently more common method of cryopreservation is vitrification, during which a cell sample suspended in a vitrification preservation medium is exposed to a cryogenic fluid or other freezing medium, such as liquid nitrogen, either by direct invasion or indirectly.
However, the existing low-temperature stem cell preservation device cannot well protect stem cell test tubes, and meanwhile cannot be stably placed in the protection cylinder, so that the stem cell test tubes in the preservation device are easy to topple over and damage after shaking.
Disclosure of Invention
The present utility model addresses the above problems by providing a stem cell cryopreservation apparatus.
The utility model is realized in such a way, the low-temperature stem cell preservation device comprises a protective outer cylinder, wherein an inner cylinder is arranged in the protective outer cylinder, a cooling cavity is formed between the inner wall of the protective outer cylinder and the inner cylinder, and a preservation device is arranged in the inner cylinder;
the preservation device comprises a placing disc and a fixing disc arranged above the placing disc, wherein a plurality of evenly distributed placing grooves are formed in the upper end face of the placing disc, a plurality of fixing holes matched with the placing grooves are formed in the upper end face of the fixing disc in a penetrating mode, two fixing plates which are oppositely arranged are arranged in the placing grooves, and springs are arranged between one ends, opposite to the other ends of the fixing plates, of the placing grooves.
In order to realize stable placement of the preservation device, as a preferred stem cell low-temperature preservation device, a plurality of protection rods which are distributed in an annular shape are fixedly connected between the upper end face of the placement disc and the fixed disc, a plurality of protection sleeves are sleeved on the outer walls of the protection rods, positioning rings are sleeved on the outer walls of the protection sleeves, four elastic columns are fixedly connected on the outer walls of the positioning rings, and four positioning grooves which are matched with the elastic columns are formed in the inner wall of the inner cylinder.
In order to support the preservation device, as a preferred embodiment of the stem cell cryopreservation device of the present utility model, a support plate is installed inside the inner cylinder, and a groove for placing a placing tray is formed in an upper end surface of the support plate.
In order to cool the stem cell test tube placed in the placing tray, as a preferred stem cell cryopreservation apparatus of the present utility model, a plurality of evenly distributed cooling pipes are installed at the bottom end inside the inner cylinder.
In order to shield and protect the stem cell test tube placed in the placing plate, the stem cell cryopreservation device is preferably provided with a plurality of fixing holes, wherein rubber pads are fixedly connected to the inner walls of the fixing holes, and a protective cover is arranged on the outer wall of the fixing plate.
In order to seal the protective outer cylinder, as a preferred low-temperature stem cell preservation device, the inner wall of the inner cylinder is in threaded connection with a protective cover extending to the upper part of the protective outer cylinder, and the upper end surface of the protective outer cylinder is fixedly connected with a handle.
In order to convey the cooling liquid nitrogen into the cooling cavity, as a stem cell cryopreservation device, preferably, a liquid inlet pipe and a liquid outlet pipe which are communicated with the cooling cavity are respectively arranged on the left side and the right side of the outer wall of the protective outer cylinder.
Compared with the prior art, the utility model has the beneficial effects that:
1. according to the low-temperature stem cell preservation device, the stem cell test tubes in the preservation device can be preserved at low temperature through the cooling cavity, a plurality of test tubes filled with stem cells are respectively placed in a plurality of placing grooves, the fixing plates are extruded according to the sizes of the test tubes, the fixing plates are stressed to extrude the springs, the springs are forced to compress until the stem cell test tubes are completely placed in the placing grooves, and meanwhile, the upper sides of the stem cell test tubes are positioned in the fixing holes, so that stable placement of the stem cell test tubes can be realized through the placing plates and the fixing plates;
2. this kind of stem cell cryopreservation device, will place dish and fixed disk through a plurality of guard bars and be connected, and then accessible a plurality of guard bars protect the stem cell test tube that the inboard was placed, avoid direct and outside contact, and can further realize the protection to the stem cell test tube through the lag, put into the inner tube with preservation device, the inner wall contact of elastic column and inner tube simultaneously, and form the extrusion to the elastic column, rotate preservation device then, make the inner wall rotation of inner tube including the elastic column, until being close to the constant head tank, and in the entering constant head tank, thereby can realize placing preservation device's stability, and to preservation device's protection, avoid receiving and rock and damage inside stem cell test tube.
Drawings
FIG. 1 is a block diagram of a stem cell cryopreservation apparatus according to the present utility model;
FIG. 2 is a cross-sectional view of a stem cell cryopreservation apparatus of the present utility model;
FIG. 3 is a block diagram of a storage device according to the present utility model;
fig. 4 is a diagram showing the construction of the placement tray and the holding tray of the present utility model.
In the figure, 1, a protective outer cylinder; 101. an inner cylinder; 102. a cooling chamber; 103. a liquid inlet pipe; 104. a liquid discharge pipe; 105. a handle; 106. a positioning groove; 107. a protective cover; 2. a storage device; 201. placing a tray; 202. a placement groove; 203. a fixing plate; 204. a spring; 205. a fixed plate; 206. a fixing hole; 207. a protective rod; 208. a protective sleeve; 209. a positioning ring; 2091. an elastic column; 2092. a protective cover; 3. a cooling tube; 4. and a support plate.
Detailed Description
The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present utility model and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model. Furthermore, in the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1-4, a low-temperature stem cell preservation device comprises a protection outer barrel 1, wherein an inner barrel 101 is arranged in the protection outer barrel 1, a cooling cavity 102 is formed between the inner wall of the protection outer barrel 1 and the inner barrel 101, and a preservation device 2 is arranged in the inner barrel 101;
the preservation device 2 comprises a placement disc 201 and a fixing disc 205 located above the placement disc 201, a plurality of evenly distributed placement grooves 202 are formed in the upper end face of the placement disc 201, a plurality of fixing holes 206 matched with the placement grooves 202 are formed in the upper end face of the fixing disc 205 in a penetrating mode, two fixing plates 203 which are oppositely arranged are arranged in the placement grooves 202, and springs 204 are arranged between one ends, opposite to the placement grooves 202, of the fixing plates 203.
In this embodiment: the stem cell test tube in the preservation device 2 can be preserved at a low temperature through the cooling cavity 102, a plurality of test tubes filled with stem cells are respectively placed in the plurality of placing grooves 202, the fixing plate 203 is extruded according to the size of the test tubes, the fixing plate 203 is stressed to extrude the spring 204, the spring 204 is forced to compress until the stem cell test tube is completely placed in the placing grooves 202, and meanwhile, the upper side of the stem cell test tube is positioned in the fixing hole 206, so that the stem cell test tube can be stably placed through the placing disc 201 and the fixing disc 205.
As a technical optimization scheme of the utility model, a plurality of protection rods 207 which are distributed in a ring shape are fixedly connected between the upper end surface of the placing tray 201 and the fixing tray 205, a protection sleeve 208 is sleeved on the outer wall of the protection rods 207, a positioning ring 209 is sleeved on the outer wall of the protection sleeve 208, four elastic columns 2091 are fixedly connected on the outer wall of the positioning ring 209, and four positioning grooves 106 which are matched with the elastic columns 2091 are formed in the inner wall of the inner cylinder 101.
In this embodiment: the placing tray 201 and the fixing tray 205 are connected through the plurality of protection rods 207, and then the stem cell test tubes placed on the inner side are protected through the plurality of protection rods 207, so that the direct contact with the outside is avoided, the protection of the stem cell test tubes can be further realized through the protection sleeve 208, the preservation device 2 is placed in the inner cylinder 101, meanwhile, the elastic column 2091 is in contact with the inner wall of the inner cylinder 101, the elastic column 2091 is extruded, then the preservation device 2 is rotated, the elastic column 2091 is rotated on the inner wall of the inner cylinder 101 until the elastic column 2091 is close to the positioning groove 106 and enters the positioning groove 106, the stable placement of the preservation device 2 can be realized, and the preservation device 2 is protected, so that the stem cell test tubes inside are prevented from being damaged due to shaking.
As a technical optimization scheme of the utility model, a supporting plate 4 is installed inside the inner cylinder 101, and a groove for placing the placing tray 201 is formed in the upper end face of the supporting plate 4.
In this embodiment: the preservation device 2 can be supported by the support plate 4, so that the preservation device 2 is placed more stably.
As a technical optimization scheme of the utility model, a plurality of cooling pipes 3 which are uniformly distributed are arranged at the bottom end inside the inner cylinder 101.
In this embodiment: the stem cell test tube placed in the placing tray 201 can be further cooled by the cooling tube 3, so that the stem cells can be kept in a low-temperature state all the time.
As a technical optimization scheme of the present utility model, rubber pads are fixedly connected to inner walls of the fixing holes 206, and a protecting cover 2092 is installed on an outer wall of the fixing plate 205.
In this embodiment: the test tube in the fixing hole 206 can be further protected by a rubber pad, and the stem cell test tube placed in the placing tray 201 can be shielded and protected by the protecting cover 2092.
As a technical optimization scheme of the utility model, the inner wall of the inner cylinder 101 is in threaded connection with a protective cover 107 extending to the upper part of the protective outer cylinder 1, and the upper end surface of the protective outer cylinder 1 is fixedly connected with a handle 105.
In this embodiment: the protection cover 107 can seal the protection outer tube 1, thereby protecting the storage device 2 in the protection outer tube 1.
As a technical optimization scheme of the utility model, a liquid inlet pipe 103 and a liquid outlet pipe 104 which are communicated with the cooling cavity 102 are respectively arranged on the left side and the right side of the outer wall of the protective outer cylinder 1.
In this embodiment: the liquid inlet pipe 103 and the liquid outlet pipe 104 are used for conveniently conveying the cooling liquid nitrogen into the cooling cavity 102, and then the cooling liquid nitrogen in the cooling cavity 102 can cool the test tube filled with the stem cells in the preservation device 2, so that the test tube can be kept in a low-temperature state.
The working principle and the using flow of the utility model are as follows: firstly, a plurality of test tubes filled with stem cells are respectively placed in a plurality of placing grooves 202, a fixing plate 203 is extruded according to the sizes of the test tubes, the fixing plate 203 is stressed to extrude a spring 204, the spring 204 is promoted to be compressed until the stem cell test tubes are completely placed in the placing grooves 202, meanwhile, the upper sides of the stem cell test tubes are positioned in fixing holes 206, next, a preservation device 2 is placed on a supporting plate 4 in an inner cylinder 101, next, the preservation device 2 is rotated, an elastic column 2091 is enabled to rotate on the inner wall of the inner cylinder 101 until the elastic column 2091 is close to a positioning groove 106 and enters the positioning groove 106, then, cooling liquid nitrogen is conveyed into a cooling cavity 102 through a liquid inlet pipe 103, low-temperature preservation of the stem cell test tubes in the preservation device 2 is achieved, and finally, a protection cover 2092 and a protection cover 107 are respectively covered on the fixing disc 205 and the inner cylinder 101.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.
Claims (7)
1. The utility model provides a stem cell cryopreservation device, includes protection urceolus (1), the inside of protection urceolus (1) is provided with inner tube (101), formed cooling chamber (102) between the inner wall of protection urceolus (1) and inner tube (101), its characterized in that: a preservation device (2) is arranged in the inner cylinder (101);
the preservation device (2) comprises a placement disc (201) and a fixing disc (205) located above the placement disc (201), wherein a plurality of evenly distributed placement grooves (202) are formed in the upper end face of the placement disc (201), a plurality of fixing holes (206) matched with the placement grooves (202) are formed in the upper end face of the fixing disc (205) in a penetrating mode, two fixing plates (203) which are oppositely arranged are arranged in the placement grooves (202), and springs (204) are arranged between one ends, opposite to the fixing plates (203), of the placement grooves (202).
2. The cryopreservation apparatus for stem cells of claim 1, wherein: the utility model discloses a protection device, including locating ring (209), inner tube (101), locating ring (209), four elastic columns (2091) are fixed connection between up end and fixed disk (205) of placing dish (201), a plurality of protection bars (207) that are annular distribution, a plurality of the outer wall of protection bars (207) has cup jointed lag (208), locating ring (209) have been cup jointed to the outer wall of lag (208), four constant head tanks (106) that mutually match with elastic columns (2091) have been seted up to the inner wall of inner tube (101).
3. The cryopreservation apparatus for stem cells of claim 1, wherein: the inner cylinder (101) is internally provided with a supporting plate (4), and the upper end surface of the supporting plate (4) is provided with a groove for placing a placing disc (201).
4. The cryopreservation apparatus for stem cells of claim 1, wherein: a plurality of cooling pipes (3) which are uniformly distributed are arranged at the bottom end inside the inner cylinder (101).
5. The cryopreservation apparatus for stem cells of claim 1, wherein: the inner walls of the fixing holes (206) are fixedly connected with rubber pads, and the outer wall of the fixing disc (205) is provided with a protective cover (2092).
6. The cryopreservation apparatus for stem cells of claim 1, wherein: the inner wall threaded connection of inner tube (101) has protective cover (107) that extends to protective urceolus (1) top, the up end fixedly connected with handle (105) of protective urceolus (1).
7. The cryopreservation apparatus for stem cells of claim 1, wherein: the left side and the right side of the outer wall of the protective outer cylinder (1) are respectively provided with a liquid inlet pipe (103) and a liquid outlet pipe (104) which are communicated with the cooling cavity (102).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321369675.4U CN219578160U (en) | 2023-06-01 | 2023-06-01 | Stem cell low-temperature preservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321369675.4U CN219578160U (en) | 2023-06-01 | 2023-06-01 | Stem cell low-temperature preservation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219578160U true CN219578160U (en) | 2023-08-25 |
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ID=87697615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321369675.4U Active CN219578160U (en) | 2023-06-01 | 2023-06-01 | Stem cell low-temperature preservation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219578160U (en) |
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2023
- 2023-06-01 CN CN202321369675.4U patent/CN219578160U/en active Active
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