CN111919837B - Biological sample cryopreservation device convenient to access - Google Patents
Biological sample cryopreservation device convenient to access Download PDFInfo
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- CN111919837B CN111919837B CN202010910211.4A CN202010910211A CN111919837B CN 111919837 B CN111919837 B CN 111919837B CN 202010910211 A CN202010910211 A CN 202010910211A CN 111919837 B CN111919837 B CN 111919837B
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- biological sample
- door
- clamped
- heat preservation
- storage container
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
Abstract
The invention discloses a biological sample cryopreservation device convenient for storing and taking, which comprises an incubator, a biological sample placing assembly and a storing and taking assembly, wherein the bottom of the incubator is provided with a base, the incubator is provided with a liquid nitrogen charging port and a sliding groove, and an independent incubator cavity is arranged in the incubator; the biological sample placing assembly is inserted into the heat preservation cavity in an inserting mode, the biological sample placing assembly comprises a placing frame and a biological sample storage container, and the biological sample storage container is clamped on the placing frame; the access assembly comprises a sliding plate, a door-shaped frame and a movable rod, the sliding plate is clamped on the sliding groove, the door-shaped frame is clamped on the sliding plate, two clamping plates are clamped at the upper end of the door-shaped frame, the movable rod is movably sleeved on a beam at the upper end of the door-shaped frame through a rotating head, and a chuck is arranged at the end part of the movable rod; the invention has reasonable structural design, is convenient for storing and taking biological samples and is suitable for large-scale popularization.
Description
Technical Field
The invention relates to the technical field of biological sample storage equipment, in particular to a biological sample cryopreservation device convenient to store and take.
Background
Biological samples are the basis of medical research, and the quality of the biological samples largely determines the accuracy and reliability of the research results. Under normal conditions, oxygen exists in the air, microorganisms in the biological sample can grow and breed when contacting with the oxygen, and the microorganisms can be effectively prevented from contacting with the oxygen through vacuum packaging, so that the breeding of the microorganisms is prevented. Cryopreservation is a commonly used method for preserving biological samples, and the biological samples are preserved by freezing through a liquid nitrogen tank.
However, the existing device for preserving the biological sample by using the liquid nitrogen needs workers to wear protective clothing when in use, and the workers directly take the biological sample by hands, and the hands of the workers are very easily frostbitten due to the extremely low temperature of the liquid nitrogen; in addition, the existing cryopreservation device is very easy to cause leakage of liquid nitrogen and influence the freezing effect of the device due to the lack of a sealing device in the process of storing and taking a biological sample.
Disclosure of Invention
Aiming at the technical problems, the invention provides the biological sample cryopreservation device which has a good preservation effect, is safe and reliable and is convenient to store and take.
The technical scheme of the invention is as follows: a biological sample freezing and storing device convenient for access comprises an incubator, a biological sample placing component and an access component; the insulation can comprises an outer box and a lining, wherein a base is arranged at the bottom of the outer box, a liquid nitrogen filling opening is formed in the outer box, sliding grooves are formed in pairs on two sides of the outer wall of the outer box, the lining is arranged in the outer box in a sleeved mode and is of a net-shaped structure, a cavity is formed between the lining and the outer box, the liquid nitrogen filling opening is communicated with the cavity, and an insulation cavity is formed in the lining; the biological sample placing assembly vertically penetrates through the outer box and is movably inserted into the heat preservation cavity, the biological sample placing assembly comprises a placing frame and a biological sample storage container, the placing frame comprises an upper pulling plate and connecting frames, the upper pulling plate is fixedly connected to the upper ends of the connecting frames, clamping grooves are formed in two sides of the upper pulling plate, the connecting frames are provided with two connecting frames, the two connecting frames are vertically and fixedly connected, sealing plates are arranged at the lower ends of the two connecting frames, the sealing plates can be clamped with openings in the upper ends of the heat preservation cavity, sample clamping plates are arranged on the two connecting frames, and the biological sample storage container is movably clamped on the sample clamping plates; the access assembly comprises two sliding plates, a door-shaped frame and movable rods, the two sliding plates are horizontally clamped on the sliding grooves through pins, the two sliding plates are respectively provided with a slot, two support legs of the door-shaped frame are movably clamped on the slots of the two sliding plates through pins, a beam at the upper end of the door-shaped frame is a columnar beam and is movably clamped with two clamping plates, the clamping plates are provided with clamping pins, and the two clamping plates are movably clamped with the clamping grooves at the two sides of the upper pulling plate through the clamping pins; the movable rod is movably sleeved on the upper end beam of the door-shaped frame through the rotating head, the end part of the movable rod is provided with a chuck, and the chuck is used for clamping a biological sample storage container.
Furthermore, the sealing plate edge is provided with a rubber sealing strip, the rubber sealing strip is movably clamped with the sealing plate through a spring rod, a corresponding sealing groove is formed in the upper end opening of the heat preservation cavity, the connecting frame rises in the process, when the sealing plate reaches the upper end opening of the heat preservation cavity, the rubber sealing strip is clamped with the sealing groove of the upper end opening of the heat preservation cavity under the action of the spring rod, the sealing performance of the device is improved, the loss of liquid nitrogen is reduced, and the freezing effect of the device is improved.
Further, the movable sleeve of chuck is established at the movable rod tip, is provided with the arc opening on the chuck, and arc opening both sides activity articulates there is the movable jaw, and the articulated department of movable jaw and arc opening is provided with the reset torsion spring, during the use, through removing the movable rod for the chuck presss from both sides biological sample storage container and gets, removes biological sample storage container through pressing down the movable jaw, and whole removal in-process, staff can not with biological sample storage container direct contact, has improved the security of device greatly.
Further, the pressure spring is sleeved on the beam at the upper end of the door-shaped frame, one end of the pressure spring is abutted to the clamping plate, the other end of the pressure spring is abutted to the beam at the upper end of the door-shaped frame, and the pressure spring is arranged, so that the clamping plate is more tightly connected with the upper pulling plate, and the reliability of the biological sample storage container in taking is improved.
Furthermore, the sliding groove is provided with a positioning hole, the sliding plate is provided with a positioning bolt, the positioning bolt can be clamped with the positioning hole, when the sliding plate rises to a specified position, the sliding plate and the sliding groove are fixed through the positioning bolt, the placing frame is further in a fixed state, the phenomenon that the placing frame falls to damage biological samples in the process of taking the biological sample storage container is avoided, and the safety of the device is improved.
Further, the lower end of the base is provided with walking wheels, and the walking wheels are arranged, so that the device can be conveniently moved and carried.
Furthermore, 3-6 heat preservation cavities are arranged, the number of the biological sample placing assemblies is consistent with the number of the heat preservation cavities correspondingly, different types of biological samples can be stored conveniently by arranging 3-6 heat preservation cavities, and the practicability of the device is improved.
The using method of the invention comprises the following steps: when the biological sample storage rack is used, a biological sample to be frozen and stored is placed in the biological sample storage container, different types of biological samples are placed in different heat preservation cavities, then the biological sample storage container is fixed on the sample clamping plate, then the biological sample storage container and the placing rack are placed in the heat preservation cavities together, liquid nitrogen is filled into the cavities through the liquid nitrogen filling openings, and the biological sample is frozen and stored; when a biological sample needs to be taken, the two clamping plates at the upper end of the door-shaped frame are clamped on the clamping grooves at the two sides of the upper pulling plate by adjusting the position of the door-shaped frame on the sliding plate, the door-shaped frame is pulled upwards, the placing frame and the biological sample storage container are pulled out of the heat preservation cavity together, when the sliding plate is lifted to a specified position, the sliding plate and the sliding groove are fixed through a positioning bolt, the biological sample storage container is clamped by using a clamping head through moving a movable rod, and the biological sample storage container is placed in an external tray for transfer through pressing a movable claw; in the ascending process of the connecting frame, when the sealing plate reaches the opening at the upper end of the heat preservation cavity, the rubber sealing strip is clamped with the sealing groove at the opening at the upper end of the heat preservation cavity under the action of the spring rod, so that the opening at the upper end of the heat preservation cavity is sealed; after the use, the positioning bolt is loosened, and the door-shaped frame is pressed down, so that the placing frame enters the heat preservation cavity again.
Compared with the prior art, the invention has the beneficial effects that: the biological sample storage container access device is reasonable in structural design, and the access assembly is arranged to access the biological sample storage container, so that personnel cannot directly contact the biological sample storage container in the whole access process, the biological sample storage container is prevented from frostbite working personnel in a low-temperature environment, and the safety of the device is greatly improved; through the arrangement of the plurality of heat preservation cavities, the device can store different types of biological samples in a classified manner, so that the practicability of the device is improved; through set up rubber seal strip on the closing plate, improved the gas tightness of device greatly, avoid the rack to remove the in-process, liquid nitrogen in the heat preservation chamber is revealed, has improved the cryopreservation effect of device.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a longitudinal section of the present invention;
FIG. 3 is a top view of the present invention;
FIG. 4 is a schematic view of the attachment of the biological specimen placement assembly of the present invention to the gantry;
FIG. 5 is a schematic view of the seal plate of the present invention;
FIG. 6 is a schematic structural view of the chuck of the present invention;
wherein, 1-heat preservation box, 10-outer box, 100-sliding groove, 1000-positioning hole, 11-inner liner, 12-base, 13-liquid nitrogen charging inlet, 14-cavity, 15-heat preservation cavity, 16-walking wheel, 2-biological sample placing component, 20-placing rack, 200-pull-up plate, 2000-clamping groove, 201-connecting rack, 202-sealing plate, 203-sample clamping plate, 204-rubber sealing strip, 2040-spring rod, 21-biological sample storage container, 3-access component, 30-sliding plate, 300-slotting, 301-positioning bolt, 31-door type rack, 310-clamping plate, 311-clamping pin, 312-pressure spring, 32-movable rod, 320-rotating head, 33-clamping head, 330-arc opening, 331-movable jaw.
Detailed Description
Example (b): the biological sample cryopreservation device convenient to access as shown in fig. 1 and 2 comprises an incubator 1, a biological sample placing assembly 2 and an access assembly 3; the heat preservation box 1 comprises an outer box 10 and a lining 11, wherein a base 12 is arranged at the bottom of the outer box 10, walking wheels 16 are arranged at the lower end of the base 12, and the walking wheels 16 are arranged, so that the device is convenient to move and transport; the outer box 10 is provided with a liquid nitrogen charging port 13, the two sides of the outer wall of the outer box 10 are provided with sliding grooves 100 in pairs, the sliding grooves 100 are provided with positioning holes 1000, the sliding plate 30 is provided with positioning bolts 301, the positioning bolts 301 can be clamped with the positioning holes 1000, when the sliding plate 30 rises to a specified position, the sliding plate 30 is fixed on the sliding grooves 100 through the positioning bolts 301, so that the placing frame 20 is in a fixed state, the phenomenon that the placing frame 20 falls to damage biological samples in the process of taking the biological sample storage container 21 is avoided, and the safety of the device is improved; the inner liner 11 is sleeved inside the outer box 10, the inner liner 11 is of a net structure, a cavity 14 is formed between the inner liner 11 and the outer box 10, a liquid nitrogen charging port 13 is communicated with the cavity 14, 6 heat preservation cavities 15 are uniformly arranged inside the inner liner 11, the number of the biological sample placing assemblies 2 is consistent with that of the heat preservation cavities 15, different types of biological samples can be conveniently stored by arranging the 6 heat preservation cavities 15, and the practicability of the device is improved;
as shown in fig. 2, 3, 4 and 5, the biological sample placing assembly 2 vertically penetrates through the outer box 10 and is movably inserted into the heat preservation cavity 15, the biological sample placing assembly 2 includes a placing rack 20 and a biological sample storage container 21, the placing rack 20 includes an upper pulling plate 200 and a connecting rack 201, the upper pulling plate 200 is fixedly connected to the upper end of the connecting rack 201, two clamping grooves 2000 are formed on two sides of the upper pulling plate 200, the connecting rack 201 is provided with two connecting racks 201, the two connecting racks 201 are vertically and fixedly connected, the lower ends of the two connecting racks 201 are both provided with a sealing plate 202, the sealing plate 202 can be clamped with the upper end opening of the heat preservation cavity 15, a rubber sealing strip 204 is arranged at the edge of the sealing plate 202, the rubber sealing strip 204 is movably clamped with the sealing plate 202 through a spring rod 2040, the upper end opening of the heat preservation cavity 15 is provided with a corresponding sealing groove, when the connecting rack 201 rises, when the sealing plate 202 reaches the upper end opening of the heat preservation cavity 15, the rubber sealing strip 204 is clamped with the sealing groove of the upper opening of the heat preservation cavity 15 under the action of the spring rod 2040, so that the sealing performance of the device is improved, the loss of liquid nitrogen is reduced, and the freezing effect of the device is improved; the two connecting frames 201 are both provided with a sample clamping plate 203, and the biological sample storage container 21 is movably clamped on the sample clamping plate 203;
as shown in fig. 1, 3, 4 and 6, the access assembly 3 includes two sliding plates 30, two door-shaped frames 31 and two movable rods 32, two ends of each sliding plate 30 are horizontally clamped on the sliding grooves 100 through pins, two slots 300 are respectively arranged on each sliding plate 30, two legs of each door-shaped frame 31 are respectively and movably clamped on the slots 300 of the two sliding plates 30 through pins, a beam at the upper end of each door-shaped frame 31 is a columnar beam and movably clamped with two clamping plates 310, each clamping plate 310 is provided with a clamping pin 311, the two clamping plates 310 are movably clamped with clamping grooves 2000 at two sides of the upper pulling plate 200 through the clamping pin 311, a pressure spring 312 is sleeved on the beam at the upper end of each door-shaped frame 31, one end of the pressure spring 312 is abutted against the clamping plate 310, the other end is abutted against the beam at the upper end of each door-shaped frame 31, and the clamping plates 310 are more tightly connected with the upper pulling plate 200 through the arrangement of the pressure spring 312, the reliability of taking the biological sample storage container is improved; the movable rod 32 is movably sleeved on an upper end beam of the door-shaped frame 31 through the rotating head 320, the end part of the movable rod 32 is provided with the chuck 33, the movable sleeve of the chuck 33 is sleeved on the end part of the movable rod 32, the chuck 33 is provided with the arc-shaped opening 330, two sides of the arc-shaped opening 330 are movably hinged with the movable claws 331, the movable claws 331 are hinged with the arc-shaped opening 330 and are provided with reset torsion springs, and during use, the movable rod 32 is moved, so that the chuck 33 clamps the biological sample storage container 21, the biological sample storage container 21 is removed through pressing the movable claws 331, and in the whole moving process, a worker can not be in direct contact with the biological sample storage container 21, and the safety of the device is greatly improved.
When the biological sample freezing and storing device is used, a biological sample to be frozen and stored is placed in the biological sample storage container 21, different types of biological samples are placed in different heat preservation cavities 15, then the biological sample storage container 21 is fixed on the sample clamping plate 203, then the biological sample storage container 21 and the placing frame 20 are placed in the heat preservation cavities 15 together, liquid nitrogen is filled into the cavity 14 through the liquid nitrogen filling port 13, and the biological sample is frozen and stored; when a biological sample needs to be taken, the two clamping plates 310 at the upper end of the door-shaped frame 31 are clamped on the clamping grooves 2000 at the two sides of the upper pulling plate 200 by adjusting the position of the door-shaped frame 31 on the sliding plate 30, the door-shaped frame 31 is pulled upwards, the placing frame 20 and the biological sample storage container 21 are pulled out of the heat preservation cavity 15 together, when the sliding plate 30 is lifted to a specified position, the sliding plate 30 and the sliding groove 100 are fixed through the positioning bolt 301, the biological sample storage container 21 is clamped through the clamping head 33 by moving the movable rod 32, and the biological sample storage container 21 is placed in an external tray for transfer by pressing the movable claw 331; in the ascending process of the connecting frame 201, when the sealing plate 202 reaches the opening at the upper end of the heat preservation cavity 15, the rubber sealing strip 204 is clamped with the sealing groove at the opening at the upper end of the heat preservation cavity 15 under the action of the spring rod 2040, so that the opening at the upper end of the heat preservation cavity 15 is sealed; after use, the positioning bolt 301 is loosened, and the door frame 31 is pressed down, so that the placing frame 20 enters the heat preservation cavity 15 again.
Claims (1)
1. A biological sample cryopreservation device convenient for access is characterized by comprising an incubator (1), a biological sample placing assembly (2) and an access assembly (3); the heat preservation box (1) comprises an outer box (10) and a lining (11), wherein a base (12) is arranged at the bottom of the outer box (10), a liquid nitrogen filling opening (13) is formed in the outer box (10), sliding grooves (100) are formed in pairs on two sides of the outer wall of the outer box (10), positioning holes (1000) are formed in the sliding grooves (100), the lining (11) is sleeved inside the outer box (10), the lining (11) is of a net-shaped structure, a cavity (14) is formed between the lining (11) and the outer box (10), the liquid nitrogen filling opening (13) is communicated with the cavity (14), and a heat preservation cavity (15) is formed inside the lining (11); the lower end of the base (12) is provided with a travelling wheel (16);
the biological sample placing component (2) vertically penetrates through the outer box (10) and is movably inserted into the heat preservation cavity (15), the biological sample placing component (2) comprises a placing rack (20) and a biological sample storage container (21), the placing rack (20) comprises an upper pulling plate (200) and a connecting rack (201), the upper pulling plate (200) is fixedly connected to the upper end of the connecting rack (201), clamping grooves (2000) are formed in two sides of the upper pulling plate (200), the connecting rack (201) is provided with two connecting racks (201), the two connecting racks (201) are vertically and fixedly connected, the lower ends of the two connecting racks (201) are respectively provided with a sealing plate (202), the sealing plate (202) can be clamped with an opening in the upper end of the heat preservation cavity (15), a rubber sealing strip (204) is arranged at the edge of the sealing plate (202), and the rubber sealing strip (204) is movably clamped with the sealing plate (202) through a spring rod (2040), a corresponding sealing groove is arranged at an opening at the upper end of the heat preservation cavity (15); sample clamping plates (203) are arranged on the two connecting frames (201), and the biological sample storage container (21) is movably clamped on the sample clamping plates (203);
the access assembly (3) comprises two sliding plates (30), a door-shaped frame (31) and a movable rod (32), two ends of each sliding plate (30) are horizontally clamped on the sliding groove (100) through pins respectively, the two sliding plates (30) are provided with open grooves (300), the sliding plates (30) are provided with positioning bolts (301), and the positioning bolts (301) can be clamped with positioning holes (1000); two support legs of the door-shaped frame (31) are movably clamped on the grooves (300) of the two sliding plates (30) through pins respectively, a beam at the upper end of the door-shaped frame (31) is a columnar beam and is movably clamped with two clamping plates (310), a pressure spring (312) is sleeved on the beam at the upper end of the door-shaped frame (31), one end of the pressure spring (312) is abutted to the clamping plates (310), and the other end of the pressure spring is abutted to the beam at the upper end of the door-shaped frame (31); the clamping plates (310) are provided with clamping pins (311), and the two clamping plates (310) are movably clamped with the clamping grooves (2000) on the two sides of the upper pulling plate (200) through the clamping pins (311); the utility model discloses a biological sample storage container, including door type frame (31), movable rod (32), chuck (33), movable jaw (330), movable jaw (331) and arc opening (330), movable jaw (331) and the articulated department of arc opening (330) are provided with the reset torsion spring, and movable rod (32) tip is provided with chuck (33) on the upper end crossbeam of door type frame (31) through rotating head (320) activity cover, chuck (33) tip is used for pressing from both sides to get biological sample storage container (21), and chuck (33) activity cover is established at movable rod (32) tip, is provided with arc opening (330) on chuck (33), arc opening (330) both sides activity is articulated to have movable jaw (331), movable jaw (331) and arc opening (330) are articulated to be provided with the reset torsion spring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010910211.4A CN111919837B (en) | 2020-09-02 | 2020-09-02 | Biological sample cryopreservation device convenient to access |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010910211.4A CN111919837B (en) | 2020-09-02 | 2020-09-02 | Biological sample cryopreservation device convenient to access |
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| CN111919837A CN111919837A (en) | 2020-11-13 |
| CN111919837B true CN111919837B (en) | 2021-11-26 |
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| CN106395223A (en) * | 2016-11-15 | 2017-02-15 | 成都叮当自动化设备有限公司 | Automatic storage and taking device for biological samples |
| CN109699635A (en) * | 2019-03-04 | 2019-05-03 | 安徽冠东电子科技有限公司 | Biological sample cryogenic storage tank and access device |
| CN111086812A (en) * | 2019-12-13 | 2020-05-01 | 苏州然成机电科技有限公司 | Biological sample storage facilities of dark low temperature |
| CN210527182U (en) * | 2019-09-29 | 2020-05-15 | 刘沁 | Biological medicine low temperature refrigeration storage device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08197388A (en) * | 1995-01-27 | 1996-08-06 | Kiriyuu Kikai Kk | Clamp device and carrying device using clamp device |
| JP5775295B2 (en) * | 2010-12-22 | 2015-09-09 | テルモ株式会社 | Membrane tissue storage and transport container and storage and transport method |
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2020
- 2020-09-02 CN CN202010910211.4A patent/CN111919837B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106081363A (en) * | 2016-06-06 | 2016-11-09 | 中国科学院合肥物质科学研究院 | A kind of liquid nitrogen zero is lost biological specimen low-temperature storing apparatus |
| CN106395223A (en) * | 2016-11-15 | 2017-02-15 | 成都叮当自动化设备有限公司 | Automatic storage and taking device for biological samples |
| CN109699635A (en) * | 2019-03-04 | 2019-05-03 | 安徽冠东电子科技有限公司 | Biological sample cryogenic storage tank and access device |
| CN210527182U (en) * | 2019-09-29 | 2020-05-15 | 刘沁 | Biological medicine low temperature refrigeration storage device |
| CN111086812A (en) * | 2019-12-13 | 2020-05-01 | 苏州然成机电科技有限公司 | Biological sample storage facilities of dark low temperature |
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