CN220663347U - Freezing preservation device for laboratory embryo - Google Patents

Abstract

The utility model relates to the technical field of embryo cryopreservation devices, in particular to a cryopreservation device for laboratory embryos, which comprises a preservation box, wherein one side of the upper surface of the preservation box is provided with an intelligent numerical control screen, the bottom end of the side surface of the preservation box is fixedly connected with a power line, the periphery of the lower surface of the preservation box is fixedly connected with a base, and one side of the preservation box, which is close to the inner part of the upper surface, is fixedly connected with a rotating shaft; in the cryopreservation device for laboratory embryos, the first tube plate and the second tube plate are arranged in the box body, so that the space inside the box body is fully occupied, the number of embryo tubes is increased, and the space utilization rate of the cryopreservation device is fully utilized; through set up the structure of first crowded backing ring, first bed rest, second crowded backing ring and second bed rest respectively in first plate body and second plate body, realized inserting embryo tube contact's stability and security.

Description

Freezing preservation device for laboratory embryo

Technical Field

The utility model relates to the technical field of embryo cryopreservation devices, in particular to a cryopreservation device for laboratory embryos.

Background

Laboratory embryo is a biological sample, adopts freezing jar or liquid nitrogen jar to preserve it under the condition of temperature at-196 ℃ generally for prevent further decomposition and the damage of cell tissue, effectively prolong the save time of cell, ensure that the embryo keeps its original vital sign in the save period, so that can successfully defrost and use when needs in the future, the program control refrigeration case that has refrigerating system that current cryopreservation device used in the experiment can accurate regulation and control cooling rate and constant temperature function, effectively avoid the ice crystal to produce, reduce the damage of embryo sample, but its equipment front is that flexible sieve pipe support places and has the unstable collision of test-tube rack and damage embryo's phenomenon.

For example, application number CN202120364255.1 discloses an embryo cryopreservation apparatus for test tube infants, which comprises a box body, a chute, a storage box, a test tube hole, a partition board, a limiting mechanism, a limiting hole, a compression rod hole, a limiting rod, a limiting spring, a compression rod, an inclined block, a button, a reset spring and a compression spring structure, wherein a plurality of storage boxes are arranged to store a plurality of embryo test tubes, the space of a liquid nitrogen tank is fully utilized, the storage boxes are in sliding connection with the chute, the storage boxes are put in or taken out of the box body only by sliding along the chute, the storage boxes are used for convenient storage and short time, a spring pressing structure is used for clamping the test tube, but the embryo test tube is pressed down forcefully to cause the recess of the tube bottom, damage the embryo structure, and the storage boxes are in close contact with the test tube, so that the defects of uneven freezing and low freezing efficiency are caused in the process that the freezing mode becomes heat transfer; the utility model provides a in the application number CN201920186996.8 discloses an embryo cryopreservation device, including the fixed plate, spacing section of thick bamboo, the silica gel pad, rectangular hole, the connecting rod, the baffle, the steel sheet, the dead lever, the rotor plate, the wheat pipe is placed the mouth, the wheat pipe sleeve, the slider, the fixture block, spacing lug, the fixture block draw-in groove, the wheat pipe, wheat Guan Pingsai and rubber lug structure, through the structural design of design fixed plate, spacing section of thick bamboo, the connecting rod, the rotor plate, the fixed lever and baffle, make this save set more convenient when taking the wheat pipe, be used for avoided the inside and outside temperature difference of liquid nitrogen jar too big and lead to the condition of embryo death to appear, work efficiency has been accelerated, and design fixed plate and spacing section of thick bamboo, the effect that the embryo wheat pipe was separately preserved has been realized, but there is freezing to hold quantity less, the unstable defect is placed to overall structure.

Therefore, both of the above-mentioned two practical information patents disclose embryo cryopreservation apparatuses, which have problems of unstable contact between embryo tube and the preserving apparatus, inactivation of embryo damage, and uneven freezing and low freezing efficiency due to insufficient space utilization of the preserving apparatus.

Disclosure of Invention

The present utility model aims to provide a cryopreservation apparatus for laboratory embryos, which solves the technical problems mentioned in the background art.

In order to achieve the above purpose, the utility model discloses a cryopreservation device for laboratory embryos, which comprises a preservation box, wherein one side of the upper surface of the preservation box is provided with an intelligent numerical control screen, the bottom end of the side surface of the preservation box is fixedly connected with a power wire, the periphery of the lower surface of the preservation box is fixedly connected with a base, one side of the interior of the preservation box, which is close to the upper surface, is fixedly connected with a rotating shaft, the inner wall of the rotating shaft is movably sleeved with a box door, the middle of the interior of the preservation box is movably connected with a first tube placing plate, the bottom surface of the interior of the preservation box is fixedly connected with a second tube placing plate, and the interiors of the first tube placing plate and the second tube placing plate are movably connected with a plurality of embryo test tubes; the structure of first tube sheet that puts is including first plate body, the equal fixedly connected with fixture block in the middle part of the positive dorsal surface of first plate body, a plurality of first tube bank has evenly been seted up to the upper surface of first plate body, the inside that first tube bank is close to the upper surface is provided with first crowded backing ring, the inside bottom of first tube bank is provided with first base pad.

Optionally, the structure of the second tube placing plate comprises a second plate body, a plurality of second tube grooves are uniformly formed in the upper surface of the second plate body, a second packing ring is arranged in the second tube grooves close to the upper surface, and a second bottom pad is arranged at the bottom end in the second tube grooves; the structure of embryo test tube is including embryo tube, embryo tube's side is provided with the scale mark, embryo tube's inner wall swing joint has the tube cap.

Optionally, the structure of preserving the case is including the box, the draw-in groove has all been seted up at the inside front and back middle part of box, the handle groove has all been seted up at the outside front and back middle part of preserving the case, the lockhole has been seted up to one side of box upper surface, is located the upper surface of box and be located the homonymy of lockhole is provided with the on & off switch, is located the inside of lockhole and be located the bottom fixedly connected with gear of on & off switch, the side of box and homonymy with the power cord are provided with the access cover, access cover and box pass through a plurality of screw activity and link to each other.

Optionally, the structure of chamber door is including the door apron, the door apron is kept away from the side middle part of pivot has seted up the lock core groove, the lock core groove is inside swing joint has the insection lock core, the handle groove has been seted up to the upper surface of door apron and one side that is close to the lock core groove.

Optionally, the material of first crowded backing ring and first collet is the same, is rubber, has elasticity, compliance, skid resistance and low thermal conductivity's characteristics for slow down external impact force, prevent rocking and the collision of test tube, first crowded backing ring comprises bottom rubber circle and top slope rubber burr fixed connection, the inner wall diameter of first crowded backing ring bottom equals with the diameter of embryo tube outer wall, but the circular diameter that its top burr point portion encloses is less than the diameter of embryo tube outer wall for insert embryo tube in the first crowded backing ring, first crowded backing ring can extrude the pipe wall, the concave curved surface of first collet with the protruding curved surface of embryo tube bottom mutually matches, is used for increasing frictional force and bottom elasticity.

Optionally, the shape of fixture block with the shape of draw-in groove is the same, and the size is unanimous each other for place first plate body in the inside centre of box, improve box space's utilization ratio and work efficiency, increase the quantity of laboratory frozen embryo, first length, width and the height of putting the tube sheet are equal with the length, width and the height that the tube sheet was put to the second respectively, just first length, the width of putting the tube sheet are equal with the inside length, the width of box respectively for with first tube sheet and the inside space of tube sheet make full use of box are put to the second, adjacent two distance between the first tube groove is 3-5mm for avoid embryo tube collision, independent embryo tube's position reduces the harm of embryo inside embryo of embryo tube.

Optionally, the tooth shape of gear with tooth shape intermeshing of tooth lock core side utilizes the on & off switch rotation to drive the gear rotation for tooth lock core carries out the flexible removal of horizontal direction, thereby realizes tooth lock core card in the inside of lockhole, reaches the state that the door apron opened and shut on the box, the shape in handle groove with the shape in handle groove is the same, and the be convenient for hold, embryo test tube's height is bigger than the height of first tube sheet that puts, but is less than 1/2 of box inside degree of depth, the diameter in first tube groove is bigger than embryo tube outer wall's diameter for make embryo tube outer wall fully contact the temperature area of freezing environment.

Advantageous effects

Compared with the prior art, the utility model has the following advantages:

1. in the cryopreservation device for laboratory embryos, the first tube plate and the second tube plate are arranged in the box body, so that the space inside the box body is fully occupied, the number of embryo tubes is increased, and the space utilization rate of the cryopreservation device is fully utilized; the first squeezing cushion ring, the first bottom cushion, the second squeezing cushion ring and the second bottom cushion are respectively arranged in the first plate body and the second plate body, so that the stability and the safety of the contact of the inserted embryo tube are realized, the embryo damage caused by the extrusion and the collision of the embryo tube by external force is avoided, and the safety and the stability of frozen embryos are improved; and by designing the diameters of the first tube groove and the second tube groove to be larger than the diameter of the outer wall of the embryo tube, uniform freezing is achieved and freezing efficiency is improved.

Drawings

FIG. 1 is a schematic diagram of the closed configuration of the cryopreservation apparatus for laboratory embryos of the present utility model.

FIG. 2 is a schematic view showing an open structure of a cryopreservation apparatus for laboratory embryos according to the present utility model.

FIG. 3 is a schematic view of the explosive structure of the cryopreservation apparatus for laboratory embryos according to the present utility model.

Fig. 4 is a longitudinal cross-sectional view of a first tubesheet structure of the present utility model.

Fig. 5 is a longitudinal cross-sectional view of the holding tank, the tank door and the second tube-placing plate structure of the present utility model.

The reference numerals are: 1. a storage case; 101. a case; 102. a clamping groove; 103. a handle slot; 104. a lock hole; 105. a switching key; 106. a gear; 107. an access cover; 108. a screw; 109. a low-temperature tank connection port; 2. an intelligent numerical control screen; 3. a power line; 4. a base; 5. a rotating shaft; 6. a door; 601. a door cover plate; 602. a lock cylinder groove; 603. a tooth lock core; 604. a handle groove; 7. a first tube plate; 701. a first plate body; 702. a clamping block; 703. a first pipe groove; 704. a first packing ring; 705. a first base pad; 8. embryo test tube; 801. embryo tube; 802. scale marks; 803. a tube cover; 9. a second tube plate; 901. a second plate body; 902. a second pipe groove; 903. a second packing ring; 904. a second bottom pad.

Detailed Description

The technical scheme of the utility model is explained in detail by specific examples.

Referring to fig. 1-5, the utility model discloses a cryopreservation device structure for laboratory embryos, which comprises a preservation box 1, wherein one side of the upper surface of the preservation box 1 is provided with an intelligent numerical control screen 2, the bottom end of the side surface of the preservation box 1 is fixedly connected with a power wire 3, the periphery of the lower surface of the preservation box 1 is fixedly connected with a base 4, one side of the interior of the preservation box 1, which is close to the upper surface, is fixedly connected with a rotating shaft 5, the inner wall of the rotating shaft 5 is movably sleeved with a box door 6, the middle of the interior of the preservation box 1 is movably connected with a first tube placing plate 7, the bottom surface of the interior of the preservation box 1 is fixedly connected with a second tube placing plate 9, and the interiors of the first tube placing plate 7 and the second tube placing plate 9 are movably connected with a plurality of embryo test tubes 8; the structure of the first tube placing plate 7 comprises a first plate body 701, wherein clamping blocks 702 are fixedly connected to the middle parts of the front side and the back side of the first plate body 701, a plurality of first tube grooves 703 are uniformly formed in the upper surface of the first plate body 701, first extrusion backing rings 704 are arranged in the first tube grooves 703 close to the upper surface, and first bottom pads 705 are arranged at the bottom ends in the first tube grooves 703.

Preferably, the structure of the second tube placing plate 9 comprises a second plate body 901, a plurality of second tube slots 902 are uniformly formed in the upper surface of the second plate body 901, a second packing ring 903 is arranged in the second tube slots 902 close to the upper surface, and a second bottom pad 904 is arranged at the bottom end in the second tube slots 902; the embryo tube 8 comprises an embryo tube 801, graduation lines 802 are arranged on the side face of the embryo tube 801, and a tube cover 803 is movably connected to the inner wall of the embryo tube 801.

Preferably, the structure of preserving case 1 is including box 101, the draw-in groove 102 has all been seted up at the inside front and back middle part of box 101, handle groove 103 has all been seted up at the outside front and back middle part of preserving case 1, lockhole 104 has been seted up to one side of box 101 upper surface, be located the upper surface of box 101 and be located lockhole 104 homonymy and be provided with on-off key 105, be located the inside of lockhole 104 and be located on-off key 105's bottom fixedly connected with gear 106, the side of box 101 and be provided with access cover 107 with power cord 3 homonymy, access cover 107 and box 101 pass through a plurality of screw 108 activity and link to each other.

Preferably, the structure of the box door 6 comprises a door cover plate 601, a lock cylinder groove 602 is formed in the middle of the side surface of the door cover plate 601 far away from the rotating shaft 5, a toothed lock cylinder 603 is movably connected inside the lock cylinder groove 602, and a handle groove 604 is formed in the upper surface of the door cover plate 601 and close to one side of the lock cylinder groove 602.

Preferably, the materials of the first extrusion backing ring 704 and the first bottom pad 705 are the same, and the first extrusion backing ring is made of rubber, has the characteristics of elasticity, softness, skid resistance and low thermal conductivity, is used for relieving external impact force, preventing shaking and collision of the test tube, helping to protect the integrity and stability of the embryo test tube, improving the safety and stability in the storage process, the first extrusion backing ring 704 is formed by fixedly connecting a bottom rubber ring and a top inclined rubber burr, the diameter of the inner wall of the bottom of the first extrusion backing ring 704 is equal to the diameter of the outer wall of the embryo tube 801, but the round diameter surrounded by the tip of the top burr is smaller than the diameter of the outer wall of the embryo tube 801, and when the embryo tube 801 is inserted into the first extrusion backing ring 704, the first extrusion backing ring 704 can extrude the tube wall, so that the stability of the embryo test tube 8 is improved, the concave curved surface of the first bottom pad 705 is matched with the convex curved surface of the bottom of the embryo tube 801, so that the friction force and the bottom elasticity are increased, the vertical storage of the test tube is ensured, and the accidental dumping risk is reduced.

Preferably, the shape of the clamping block 702 is the same as that of the clamping groove 102, the clamping blocks are consistent in size, the first plate 701 is placed in the middle of the inside of the box 101, the utilization rate and the working efficiency of the space of the box 101 are improved, the number of frozen embryos in a laboratory is increased, the length, the width and the height of the first tube placing plate 7 are respectively equal to those of the second tube placing plate 9, the length and the width of the first tube placing plate 7 are respectively equal to those of the inside of the box 101, the space of the box 101 is fully utilized by the first tube placing plate 7 and the second tube placing plate 9, the distance between two adjacent first tube grooves 703 is 3-5mm, the collision of the embryo tubes 801 is avoided, the positions of the embryo tubes 801 are independent, and the damage of the embryos in the embryo tubes 801 is reduced.

Preferably, the tooth shape of the gear 106 is meshed with the tooth shape of the side surface of the tooth lock cylinder 603, the gear 106 is driven to rotate by using the rotation of the switch key 105, so that the tooth lock cylinder 603 stretches and contracts horizontally, the tooth lock cylinder 603 is clamped in the lock hole 104, the door cover plate 601 is opened and closed on the box body 101, the shape of the handle groove 604 is the same as that of the handle groove 103, the holding is convenient, the height of the embryo test tube 8 is larger than that of the first tube placing plate 7, but smaller than 1/2 of the depth inside the box body 101, the diameter of the first tube groove 703 is larger than that of the outer wall of the embryo tube 801, and the outer wall of the embryo tube 801 is enabled to be fully contacted with the temperature area of the freezing environment, and the freezing efficiency is improved.

Working principle: firstly, a worker in a laboratory holds a handle groove 103 on the side surface of a handheld box body 101 at a proper place, so that a base 4 is placed on a platform, a low-temperature liquid nitrogen tank is taken out, a pipeline of the low-temperature liquid nitrogen tank is connected in a low-temperature tank connecting port 109 on the side surface of the box body 101 in a sealing way, a power line 3 is connected with a power supply to start an intelligent numerical control screen 2 for refrigerating operation, a switch key 105 is screwed by hands to drive a gear 106 to rotate, a toothed lock cylinder 603 meshed on the gear 106 translates in a lock cylinder groove 602, a door cover plate 601 is opened by utilizing the handheld handle groove 604 and is turned over, the door cover plate 601 is connected to the side part of the inner wall of a rotating shaft 5, the upper surface of the box body 101 is opened, a first tube placing plate 7 is taken out by utilizing a clamp, a clamping block 702 is longitudinally and vertically taken out from the clamping groove 102, a second tube placing plate 9 at the bottom is leaked, and an embryo which is still active is taken out in the laboratory, after the embryo tube 801 is sealed by the tube cover 803, the embryo tube 801 is sequentially inserted into the second tube slot 902 on the second plate body 901, when the embryo tube 801 is inserted, the side wall of the embryo tube 801 is stably extruded by the second extrusion backing ring 903 and the second bottom pad 904 to be in a balanced state, after the second tube placing plate 9 is fully inserted, the clamping block 702 on the first plate body 701 is put down along the clamping slot 102 by using the clamp, then the embryo tube 8 for storing the embryo is inserted into the first tube slot 703 on the first plate body 701 as prepared, likewise, the first extrusion backing ring 704 and the first bottom pad 705 are tightly clamped on the outer wall of the embryo tube 801 and are stable and do not collide, after the first tube placing plate 7 is fully inserted, a worker holds the handle slot 604 to close the door cover plate 601, so that the tooth lock cylinder 603 is put down along the lock hole 104, then the gear 106 controlled by the switch key 105 is screwed to engage the tooth 603, so that the embryo tube 8 is plugged into the lock cylinder 104, the door 6 is closed, finally, the intelligent numerical control screen 2 is started to enable the inside of the box body 101 to be frozen and kept at the constant temperature of-196 ℃.

The above description is only of the preferred embodiments of the present utility model, and is not intended to limit the utility model, but any modifications, equivalent substitutions, improvements, etc. within the design concept of the present utility model should be included in the scope of the present utility model.

Claims (7)

1. A cryopreservation apparatus for laboratory embryos, characterized in that: the intelligent numerical control machine comprises a preservation box (1), one side of the upper surface of the preservation box (1) is provided with an intelligent numerical control screen (2), the bottom end of the side surface of the preservation box (1) is fixedly connected with a power line (3), the periphery of the lower surface of the preservation box (1) is fixedly connected with a base (4), one side of the interior of the preservation box (1) close to the upper surface is fixedly connected with a rotating shaft (5), the inner wall of the rotating shaft (5) is movably sleeved with a box door (6), the middle of the interior of the preservation box (1) is movably connected with a first tube placing plate (7), the bottom surface of the interior of the preservation box (1) is fixedly connected with a second tube placing plate (9), and a plurality of embryo test tubes (8) are movably connected to the interior of the first tube placing plate (7) and the second tube placing plate (9); the structure of first tube placing plate (7) is including first plate body (701), the equal fixedly connected with fixture block (702) in middle part of the positive dorsal surface of first plate body (701), a plurality of first piping groove (703) have evenly been seted up to the upper surface of first plate body (701), the inside that first piping groove (703) is close to the upper surface is provided with first crowded backing ring (704), the inside bottom of first piping groove (703) is provided with first base pad (705).

2. A cryopreservation apparatus for laboratory embryos according to claim 1, wherein: the structure of the second tube placing plate (9) comprises a second plate body (901), a plurality of second tube grooves (902) are uniformly formed in the upper surface of the second plate body (901), a second packing ring (903) is arranged in the second tube grooves (902) close to the upper surface, and a second bottom pad (904) is arranged at the bottom end in the second tube grooves (902); the structure of embryo test tube (8) is including embryo tube (801), the side of embryo tube (801) is provided with scale mark (802), the inner wall swing joint of embryo tube (801) has tube cap (803).

3. A cryopreservation apparatus for laboratory embryos according to claim 2, wherein: the structure of save case (1) is including box (101), draw-in groove (102) have all been seted up at the inside front and back middle part of box (101), handle groove (103) have all been seted up at the outside front and back middle part of save case (1), lockhole (104) have been seted up to one side of box (101) upper surface, are located the upper surface of box (101) and are located the homonymy of lockhole (104) is provided with on & off switch (105), is located the inside of lockhole (104) and be located the bottom fixedly connected with gear (106) of on & off switch (105), the side of box (101) and with power cord (3) homonymy are provided with access cover (107), access cover (107) and box (101) link to each other through a plurality of screw (108) activity.

4. A cryopreservation apparatus for laboratory embryos according to claim 3, wherein: the structure of chamber door (6) is including door apron (601), door apron (601) keep away from lock core groove (602) have been seted up at the side middle part of pivot (5), lock core groove (602) are inside swing joint has tooth line lock core (603), handle groove (604) have been seted up on the upper surface of door apron (601) and one side that is close to lock core groove (602).

5. A cryopreservation apparatus for laboratory embryos as claimed in claim 4 wherein: the material of first crowded backing ring (704) is the same with first collet (705), first crowded backing ring (704) comprises bottom rubber circle and top slope rubber burr fixed connection, the inner wall diameter of first crowded backing ring (704) bottom equals with embryo pipe (801) outer wall's diameter, but the circular diameter that its top burr point portion encloses is less than the diameter of embryo pipe (801) outer wall, the concave curved surface of first collet (705) with the protruding curved surface of embryo pipe (801) bottom matches each other.

6. A cryopreservation apparatus for laboratory embryos as claimed in claim 4 wherein: the shape of the clamping block (702) is the same as that of the clamping groove (102), the sizes of the clamping blocks are consistent with each other, the length, the width and the height of the first tube placing plate (7) are respectively equal to those of the second tube placing plate (9), the length and the width of the first tube placing plate (7) are respectively equal to those of the inside of the box body (101), and the distance between two adjacent first tube grooves (703) is 3-5mm.

7. A cryopreservation apparatus for laboratory embryos as claimed in claim 4 wherein: the tooth shape of the gear (106) is meshed with the tooth shape of the side face of the tooth lock cylinder (603), the shape of the handle groove (604) is the same as that of the handle groove (103), the height of the embryo test tube (8) is larger than that of the first tube placing plate (7) but smaller than 1/2 of the inner depth of the box body (101), and the diameter of the first tube groove (703) is larger than that of the outer wall of the embryo tube (801).