CN216853590U

CN216853590U - Mouse bone marrow stem cell preservation device

Info

Publication number: CN216853590U
Application number: CN202220403975.9U
Authority: CN
Inventors: 姜红; 魏强
Original assignee: Chengdu Fushi Biotechnology Co ltd
Current assignee: Chengdu Fushi Biotechnology Co ltd
Priority date: 2022-02-25
Filing date: 2022-02-25
Publication date: 2022-07-01
Anticipated expiration: 2032-02-25

Abstract

The utility model discloses a mouse bone marrow stem cell preservation device, which comprises a first shell, wherein a second shell is arranged in the first shell, a third shell is arranged in the second shell, a vacuum cavity is formed between the first shell and the second shell, a freezing cavity is formed between the second shell and the third shell, mounting plates are arranged above and below the inside of the third shell, a protective tube is fixedly arranged on the upper end surface of the upper mounting plate in a penetrating way, when in use, the reagent tube is protected by the rubber layer in the protective tube, so that the reagent tube can be prevented from being damaged, then to freezing intracavity portion packing liquid nitrogen, carry out low temperature protection to the intraduct cell of reagent through the liquid nitrogen, the vacuum cavity of setting can improve this cell holding device's thermal insulation performance, and a plurality of damper units of setting can carry out further shock attenuation protection to the reagent pipe, further avoids the reagent pipe to take place to damage.

Description

Mouse marrow stem cell preservation device

Technical Field

The utility model relates to the technical field of cell preservation, in particular to a mouse bone marrow stem cell preservation device.

Background

The mouse bone marrow cells comprise hematopoietic stem cells, bone marrow stromal stem cells, endothelial cells and the like, wherein the hematopoietic stem cells have high division capacity and can generate various blood cells and primitive cells, and the bone marrow stromal stem cells are stem cell groups with multidirectional differentiation potential stored in bone marrow and can be differentiated into osteocytes, chondrocytes, cardiomyocytes, hepatocytes, neurons, glial cells and vascular endothelial cells in vitro or in vivo under certain conditions.

The existing device for preserving the mouse bone marrow stem cells is simple in structure, single in use performance, easy to damage in the preservation and transportation process and capable of influencing the use effect due to the fact that most of devices are stored by using test tubes, and therefore a certain limitation exists.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to a mouse bone marrow stem cell preservation device to solve the above problems of the background art.

In order to achieve the purpose, the utility model provides the following technical scheme:

a mouse bone marrow stem cell preservation device comprises a first shell, wherein a second shell is arranged inside the first shell, a third shell is arranged inside the second shell, a vacuum cavity is formed between the first shell and the second shell, a freezing cavity is formed between the second shell and the third shell, a liquid inlet nitrogen pipe and a liquid outlet nitrogen pipe which are communicated with the freezing cavity are connected onto the third shell, the outer surfaces of the liquid inlet nitrogen pipe and the liquid outlet nitrogen pipe are both in threaded connection with sealing plugs, the upper end surface of the third shell is hinged with a sealing plate, mounting plates are arranged above and below the inside of the third shell, guide rails are respectively arranged on the front side and the rear side of the inner left side wall and the front side and the rear side of the inner right side wall of the third shell, slide blocks are fixedly connected on the front side and the rear side of the left end surface and the front side and the rear side of the right end surface of the mounting plates, the slide blocks are connected with the corresponding guide rails in a sliding manner, and a plurality of fixing rods are fixedly connected between the mounting plates above and below, the fixed protection tube that wears to be equipped with of up end of top mounting panel, the bottom of protection tube and the up end fixed connection of below mounting panel, the inner wall fixedly connected with rubber layer of protection tube, the inside on rubber layer is formed with the reagent pipe and places the chamber, the surface threaded connection of protection tube has sealed lid, a plurality of damper of fixedly connected with rubber block, fixedly connected with between the bottom face of below mounting panel and the interior bottom end lateral wall of third casing in the bottom face middle part of sealed lid.

Preferably, a handle is installed in the middle of the upper end face of the sealing plate, and an anti-slip sleeve is fixedly sleeved in the middle of the outer surface of the handle.

Preferably, the damping component comprises an installation disc, the installation disc is in bolted connection with the inner bottom side wall of the third shell, a supporting cylinder is fixedly connected to the middle of the upper end face of the installation disc, a lifting block is slidably connected to the inside of the supporting cylinder, a supporting rod is slidably penetrated through the top end of the supporting cylinder, a fixed disc is fixedly connected to the top end of the supporting rod, the fixed disc is in bolted connection with the bottom end face of the lower installation plate, the bottom end of the supporting rod is fixedly connected with the upper end face of the lifting block, a first spring is fixedly connected between the lifting block and the inner bottom side wall of the supporting cylinder, a plurality of annularly distributed sliding grooves are formed in the upper end face of the installation disc, a moving block is slidably connected to the sliding grooves, a connecting rod is hinged to the upper end face of the moving block, a lifting ring block is slidably sleeved on the outer surface of the supporting cylinder, and the top end of the connecting rod is hinged to the outer surface of the lifting ring block, the fixed connecting rod is fixedly connected with the second spring between the connecting rod and the supporting cylinder, and the fixed disc is fixedly connected with the fixed sleeve between the fixed disc and the lifting ring block.

Preferably, the fixed sleeve is sleeved on the periphery of the support rod.

Preferably, the freezing chamber is internally filled with liquid nitrogen.

Preferably, the outer surface of the supporting rod is sleeved with a third spring, one end of the third spring is fixedly connected with the bottom end face of the fixed disc, and the other end of the third spring is fixedly connected with the upper end face of the supporting cylinder.

Compared with the prior art, the utility model has the beneficial effects that: the cell preservation device is convenient to use and simple to operate, when the cell preservation device is used, the reagent tube filled with the mouse bone marrow stem cells is placed inside the protection tube, the sealing cover is screwed on, the reagent tube is protected by the rubber layer inside the protection tube, the reagent tube can be prevented from being damaged, then liquid nitrogen is filled into the freezing cavity, the cells inside the reagent tube are subjected to low-temperature protection by the liquid nitrogen, the arranged vacuum cavity can improve the heat insulation performance of the cell preservation device, the arranged damping components can further perform damping protection on the reagent tube, the reagent tube is further prevented from being damaged, and the protection tube can be stably lifted in the damping process by the sliding fit of the guide rail and the sliding block; the portability of the cell holding device can be improved through the handle; when the cell preservation device absorbs shock, the supporting rod drives the lifting block to slide up and down along the inner wall of the supporting cylinder, the first spring is compressed or stretched at the moment, when the supporting rod moves downwards, the lifting ring block is driven to move downwards through the fixing sleeve, when the lifting ring block moves downwards, the moving block is driven to slide along the inner wall of the sliding groove through the connecting rod, the second spring is stretched or compressed at the moment, and the cell preservation device has shock absorption and buffering capacity through the cooperation of the first spring and the second spring; when the bracing piece up-and-down motion, the third spring will be stretched or compressed, and then can improve this cell save set's shock attenuation buffering effect through the third spring.

Drawings

FIG. 1 is a schematic cross-sectional front view of a main body structure of a mouse bone marrow stem cell preservation device;

FIG. 2 is a schematic view of a shock-absorbing assembly of a mouse bone marrow stem cell preservation device.

In the figure: 1-a first shell, 2-a vacuum cavity, 3-a freezing cavity, 4-a shock-absorbing component, 5-a sliding block, 6-a fixing rod, 7-a guide rail, 8-a sealing plug, 9-a sealing plate, 10-a rubber block, 11-a handle, 12-a sealing cover, 13-a mounting plate, 14-a rubber layer, 15-a protective tube, 16-a third shell, 17-a second shell, 18-a support rod, 19-a fixed disk, 20-a third spring, 21-a fixing sleeve, 22-a lifting block, 23-a second spring, 24-a connecting rod, 25-a sliding chute, 26-a moving block, 27-a mounting disk, 28-a first spring, 29-a support barrel and 30-a lifting ring block.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1: referring to fig. 1-2, a mouse bone marrow stem cell preservation device comprises a first shell 1, a second shell 17 is arranged inside the first shell 1, a third shell 16 is arranged inside the second shell 17, a vacuum cavity 2 is formed between the first shell 1 and the second shell 17, a freezing cavity 3 is formed between the second shell 17 and the third shell 16, a liquid inlet nitrogen pipe and a liquid outlet nitrogen pipe which are communicated with the freezing cavity 3 are connected to the third shell 16, sealing plugs 8 are respectively connected to the outer surfaces of the liquid inlet nitrogen pipe and the liquid outlet nitrogen pipe through threads, a sealing plate 9 is hinged to the upper end surface of the third shell 16, mounting plates 13 are respectively arranged above and below the inside of the third shell 16, guide rails 7 are respectively arranged on the front and rear sides of the inner left side wall and the front and rear sides of the inner right side wall of the third shell 16, sliders 5 are respectively fixedly connected to the front and rear sides of the left end surface and the front and rear sides of the right end surface of the mounting plate 13, slider 5 and the guide rail 7 sliding connection who corresponds, a plurality of dead levers 6 of fixedly connected with between the mounting panel 13 of upper and lower side, protection tube 15 is worn to be equipped with fixedly to the up end of top mounting panel 13, the bottom of protection tube 15 and the up end fixed connection of below mounting panel 13, the inner wall fixedly connected with rubber layer 14 of protection tube 15, the inside on rubber layer 14 is formed with the reagent pipe and places the chamber, the surface threaded connection of protection tube 15 has sealed lid 12, the bottom face middle part fixedly connected with block rubber 10 of sealed lid 12, a plurality of damper 4 of fixedly connected with between the bottom face of below mounting panel 13 and the interior bottom end lateral wall of third casing 16.

During the use, the inside of protection tube 15 is placed to the reagent pipe that will be equipped with mouse marrow stem cell, close sealed lid 12 soon, protect the reagent pipe through the inside rubber layer 14 of protection tube 15, can avoid reagent pipe to take place to damage, then to freezing 3 inside liquid nitrogen that fill in chambeies, carry out low temperature protection to the inside cell of reagent pipe through the liquid nitrogen, the vacuum chamber 2 that sets up can improve this cell holding device's thermal insulation performance, a plurality of damper 4 that set up can carry out further shock attenuation protection to the reagent pipe, further avoid reagent pipe to take place to damage, can make protection tube 15 stabilize the lift at the shock attenuation in-process through

guide rail

7 and 5 sliding fit of slider.

The middle part of the upper end face of the sealing plate 9 is provided with a handle 11, and the middle part of the outer surface of the handle 11 is fixedly sleeved with an anti-skidding sleeve.

The portability of the cell holding device can be increased by the handle 11.

Wherein, the damping component 4 includes a mounting disc 27, the mounting disc 27 is bolted with the inner bottom end side wall of the third casing 16, the middle part of the upper end surface of the mounting disc 27 is fixedly connected with a supporting cylinder 29, the inside of the supporting cylinder 29 is slidably connected with a lifting block 22, the top end of the supporting cylinder 29 is slidably penetrated with a supporting rod 18, the top end of the supporting rod 18 is fixedly connected with a fixed disc 19, the fixed disc 19 is bolted with the bottom end surface of the lower mounting plate 13, the bottom end of the supporting rod 18 is fixedly connected with the upper end surface of the lifting block 22, a first spring 28 is fixedly connected between the lifting block 22 and the inner bottom end side wall of the supporting cylinder 29, the upper end surface of the mounting disc 27 is provided with a plurality of annularly distributed chutes 25, the chutes 25 are slidably connected with moving blocks 26, the upper end surface of the moving blocks 26 is hinged with a connecting rod 24, the outer surface of the supporting cylinder 29 is slidably sleeved with a lifting ring 30, the top of connecting rod 24 is articulated with the surface of lift ring piece 30, fixedly connected with second spring 23 between connecting rod 24 and the support cylinder 29, fixed cover 21 of fixed connection between fixed disk 19 and the lift ring piece 30.

During the shock attenuation, bracing piece 18 will drive elevator block 22 and slide from top to bottom along a support section of thick bamboo 29 inner wall, first spring 28 will be compressed or tensile this moment, and when bracing piece 18 moves down, drive elevator ring piece 30 through fixed cover 21 and move down, will drive movable block 26 through connecting rod 24 and slide along spout 25 inner wall when elevator ring piece 30 moves down, and second spring 23 will be stretched or compressed this moment, and then can make this cell preservation device have the shock attenuation buffer capacity through first spring 28 and the cooperation of second spring 23.

Wherein, the fixed sleeve 21 is sleeved on the periphery of the support bar 18.

Wherein the freezing chamber 3 is filled with liquid nitrogen.

Example 2: referring to fig. 1-2, a device for preserving mouse bone marrow stem cells is different from embodiment 1 in that a third spring 20 is sleeved on an outer surface of a support rod 18, one end of the third spring 20 is fixedly connected to a bottom end surface of a fixed plate 19, and the other end of the third spring 20 is fixedly connected to an upper end surface of a support cylinder 29.

When the support rod 18 moves up and down, the third spring 20 will be stretched or compressed, and the third spring 20 can improve the damping and buffering effect of the cell preservation device.

In the present invention, terms such as "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "side", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only terms of relationships determined for convenience of describing structural relationships of the parts or elements of the present invention, and are not intended to refer to any parts or elements of the present invention, and are not to be construed as limiting the present invention.

Claims

1. A mouse bone marrow stem cell preservation device comprises a first shell (1) and is characterized in that: the refrigerator is characterized in that a second shell (17) is arranged inside the first shell (1), a third shell (16) is arranged inside the second shell (17), a vacuum cavity (2) is formed between the first shell (1) and the second shell (17), a freezing cavity (3) is formed between the second shell (17) and the third shell (16), a liquid inlet nitrogen pipe and a liquid outlet nitrogen pipe which are communicated with the freezing cavity (3) are connected onto the third shell (16), sealing plugs (8) are in threaded connection with the outer surfaces of the liquid inlet nitrogen pipe and the liquid outlet nitrogen pipe respectively, sealing plates (9) are hinged to the upper end face of the third shell (16), mounting plates (13) are arranged above and below the inside of the third shell (16), guide rails (7) are arranged on the front side and the rear side of the inner left side wall and the front side and the rear side of the inner right side wall of the third shell (16), and sliders (5) are fixedly connected to the front side and the rear side of the left end face and the front side of the right end face of the mounting plate (13), slider (5) and guide rail (7) sliding connection who corresponds, a plurality of dead levers of fixedly connected with (6) between mounting panel (13) of upper and lower side, protection tube (15) are worn to be equipped with to the up end of top mounting panel (13) is fixed, the bottom of protection tube (15) and the up end fixed connection of below mounting panel (13), the inner wall fixedly connected with rubber layer (14) of protection tube (15), the inside of rubber layer (14) is formed with the reagent pipe and places the chamber, the surface threaded connection of protection tube (15) has sealed lid (12), the bottom face middle part fixedly connected with rubber block (10) of sealed lid (12), a plurality of damper (4) of fixedly connected with between the bottom face of below mounting panel (13) and the interior bottom end lateral wall of third casing (16).

2. The device of claim 1, wherein the stem cell preservation device comprises: the middle part of the upper end surface of the sealing plate (9) is provided with a handle (11), and the middle part of the outer surface of the handle (11) is fixedly sleeved with an anti-skidding sleeve.

3. The device of claim 2, wherein the stem cell preservation device comprises: the shock absorption assembly (4) comprises a mounting disc (27), the mounting disc (27) is in bolted connection with the inner bottom side end side wall of the third shell (16), a supporting cylinder (29) is fixedly connected to the middle of the upper end face of the mounting disc (27), a lifting block (22) is connected to the inside of the supporting cylinder (29) in a sliding manner, a supporting rod (18) is arranged on the top end of the supporting cylinder (29) in a sliding manner, a fixed disc (19) is fixedly connected to the top end of the supporting rod (18), the fixed disc (19) is in bolted connection with the bottom end face of the mounting plate (13) below, the bottom end of the supporting rod (18) is fixedly connected with the upper end face of the lifting block (22), a first spring (28) is fixedly connected between the inner bottom side end side wall of the lifting block (22) and the supporting cylinder (29), and a plurality of annularly distributed sliding grooves (25) are formed in the upper end face of the mounting disc (27), the improved lifting device is characterized in that a moving block (26) is connected to the sliding groove (25) in a sliding mode, a connecting rod (24) is hinged to the upper end face of the moving block (26), a lifting ring block (30) is sleeved on the outer surface of the supporting cylinder (29) in a sliding mode, the top end of the connecting rod (24) is hinged to the outer surface of the lifting ring block (30), a second spring (23) is fixedly connected between the connecting rod (24) and the supporting cylinder (29), and a fixing sleeve (21) is fixedly connected between the fixing disc (19) and the lifting ring block (30).

4. The device of claim 3, wherein the stem cell preservation device comprises: the fixed sleeve (21) is sleeved on the periphery of the support rod (18).

5. The device of claim 4, wherein the stem cell preservation device comprises: the freezing cavity (3) is filled with liquid nitrogen.

6. The device of claim 5, wherein the stem cell preservation device comprises: the outer surface of the supporting rod (18) is sleeved with a third spring (20), one end of the third spring (20) is fixedly connected with the bottom end face of the fixed disc (19), and the other end of the third spring (20) is fixedly connected with the upper end face of the supporting cylinder (29).