CN219526642U - Constant-temperature water bag heating device for cell experiment - Google Patents
Constant-temperature water bag heating device for cell experiment Download PDFInfo
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- CN219526642U CN219526642U CN202320306418.XU CN202320306418U CN219526642U CN 219526642 U CN219526642 U CN 219526642U CN 202320306418 U CN202320306418 U CN 202320306418U CN 219526642 U CN219526642 U CN 219526642U
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Abstract
The utility model relates to the technical field of biotechnology scientific research, in particular to a constant-temperature water bag heating device for cell experiments. The technical proposal comprises: including stainless steel shell and base, be provided with the water bag in stainless steel shell, the inside aseptic water that fills up of water bag is provided with rotational speed adjusting device, temperature monitoring device, timing device in the base, rotational speed adjusting device includes the axis of rotation, rotating motor and the rotational speed adjust knob that is located the base outside, temperature monitoring device is including setting up temperature display, temperature setting button and the temperature monitor at the base front surface, still be provided with more than one position hole, two heating rods in the water bag, be provided with the water hole of changing that stretches out stainless steel shell at the side of water bag. The structure is provided with the independent sealing water bag, and only the constant-temperature heating water bag is needed to complete heat transfer, so that water source or cell pollution is avoided.
Description
Technical Field
The utility model relates to the technical field of biotechnology scientific research, in particular to a constant-temperature water bag heating device for cell experiments, which is used for cell resuscitation or protein inactivation aseptic experiments, and belongs to the technical field of cell aseptic experiments.
Background
The constant temperature bath is mainly applied to cell culture, clinical test, genetic immunity, plant chemical industry, factory enterprise laboratories and the like in bioengineering, is experimental equipment used in the fields of biochemistry and medicine, and mainly comprises two methods: constant temperature water bath heating and constant temperature metal bath heating, wherein:
the general open thermostatic waterbath has the following problems: bacteria are more easy to breed, especially in the stem cell cultivation step, the water in the constant-temperature water bath is in a constant-temperature state suitable for bacterial growth for a long time and is very sensitive to bacterial and other pollutants, and the open constant-temperature water bath is not suitable for the long-time stem cell cultivation requirement. And the traditional water bath heating can cause water leakage and water seepage to possibly cause electric shock to people, thereby affecting the health and safety of people. The metal bath is heated by using a metal module, but the germs possibly flow out when the germs are cultivated in the existing metal bath product, and the generated toxic substances can influence the health and safety of human bodies. Although the heat conduction speed of the metal bath is high, the temperature gradient is generated, the contact position between the middle of the metal block and devices such as test tubes is slightly lower than the peripheral temperature, and a temperature gradient is generated; moreover, one metal block corresponds to one size caliber instrument, so that a plurality of cultivation instruments with different sizes and diameters can not be used simultaneously, and aiming at test tubes and culture dishes with nonstandard diameters, the metal bath of the finished product can not be met, and the cost for automatically manufacturing a whole metal block is too high;
whereas the existing common constant temperature bath has the following problems: the conduction medium in the thermostatic water bath is sterile water, but when the thermostatic water bath is used, the water source is exposed in an unclean environment and is easy to grow bacteria, in the operation step of stem cell culture, the pollution sources are easy to grow due to the fact that the pollution sources such as bacteria are grown, therefore, the open thermostatic water bath is not suitable for long-time cultivation of stem cells, when the cells are recovered by operation, an operator needs to continuously hold the cells in the sterile water medium to shake continuously, so that the heat transfer of the sterile water medium is stable, the cells in the hands are kept in a thermostatic water bath state, the defects are that the hands of the operator cannot be liberated, meanwhile, the operator can be easily scalded by using the open water bath for a long time, and the thermostatic water bath can possibly generate water leakage and water seepage in a clean area environment, or can possibly cause electric shock to the operator in the operation experiment process.
In summary, both of the existing methods have the pollution problems of water source pollution or secondary pollution of cells.
Disclosure of Invention
The utility model aims to solve the problems of water source pollution or secondary pollution of cells in the background technology, and provides a constant-temperature water bag heating device for cell experiments.
The technical scheme of the utility model is as follows: the utility model provides a cell experiment uses constant temperature water bag heating device, includes stainless steel shell and base, its characterized in that: the stainless steel shell is of a cube-shaped non-cover structure, a water bag of a cube-shaped structure is arranged in the stainless steel shell, sterile water is filled in the water bag, a rotating speed adjusting device, a temperature monitoring device and a timing device are arranged in the base, the rotating speed adjusting device comprises four rotating shafts arranged at the bottom of the stainless steel shell, a rotating motor fixed in the base and connected with the rotating shafts in a one-to-one correspondence manner and a rotating speed adjusting knob arranged on the outer side of the base, the temperature monitoring device comprises a temperature display, a temperature setting button and a temperature monitor, the temperature monitor is arranged on the front surface of the base and used for realizing constant-temperature heating, more than one position hole and two heating rods are further arranged in the water bag, and water changing holes extending out of the stainless steel shell are formed in the side edge of the water bag.
Preferably, the base is made of stainless steel and has a trapezoid cubic structure.
Preferably, the position holes are respectively six 50ml centrifuge tube position holes with the caliber of 28.6mm and eight cryopreservation tube position holes with the caliber of 6.5mm, each position hole is provided with a position hole heat preservation cover, and the size of each position hole heat preservation cover is consistent with that of the corresponding position hole.
Preferably, the base is provided with a main switch.
Preferably, four balance supporting feet are arranged at the bottom of the base.
Compared with the prior art, the utility model has the following beneficial technical effects:
1. the heat conducting medium in the water bag is sterile water: the medium has excellent heat conductivity and low cost, and can be replaced by new sterile water at any time;
2. sterile water in the water bag can be replaced at any time;
3. the base is provided with an oscillating device: when the operation is performed to recover cells, hands of an operator do not need to shake continuously in the sterile water medium, and the water bag bottom oscillating device of the device continuously oscillates the water bag in an electric control mode so as to keep stable heat transfer of the sterile water medium in the water bag, keep the medium in the water bag in a constant-temperature state, liberate the hands of the operator, and simultaneously avoid the condition that the operator is easy to scald the hands when using an open water bath for a long time;
4. setting a time timing device: the device has the functions of countdown and stopwatch, so that the distraction and error of operators in the operation process are reduced; meanwhile, time is saved, and working efficiency is improved;
5. the plurality of caliber holes are arranged, so that the freezing storage tubes with different diameters can be realized, and the centrifuge tubes are used at the same time, so that the time is saved, and the working efficiency of operators is improved;
6. the heating device is provided with an independent sealing water bag, and only the constant-temperature heating water bag is needed to complete heat transfer, so that water source or cell pollution is avoided;
7. the utility model adopts the sealed water bag, avoids the contact of thalli, does not form a fungus membrane layer and does not need cleaning.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a heating device for a constant temperature water bag for cell experiment in example 1;
FIG. 2 is a top view of the base of example 1;
FIG. 3 is a view showing the construction of the inside of the water bag of embodiment 1;
FIG. 4 is a schematic diagram showing the internal structure of a heating device for a constant temperature water bag used in a cell experiment in example 1.
Reference numerals: stainless steel shell 1, base 2, water bag 3, position hole 4, position hole heat preservation lid 5, trade water hole 6, heating rod 7, temperature monitor 8, axis of rotation 9, master switch 10, temperature monitoring device 11, rotational speed adjusting device 12, timing device 13, balanced supporting legs 14, rotating motor 15, temperature display 11-1, temperature setting button 11-2.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions in the embodiments of the present utility model will be clearly and completely described in the following in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without inventive faculty, are intended to be within the scope of the present utility model, based on the embodiments of the present utility model.
In the description of the present utility model, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present utility model and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Example 1
As shown in fig. 1 to 4, the utility model provides a heating device for a constant temperature water bag for cell experiments, which comprises a stainless steel shell 1 and a base 2, and is characterized in that: the stainless steel shell 1 is of a cube-shaped non-cover structure, a water bag 3 of a cube-shaped structure is arranged in the stainless steel shell 1, sterile water is filled in the water bag 3, a rotating speed adjusting device 12, a temperature monitoring device 11 and a timing device 13 are arranged in the base 2, the rotating speed adjusting device 12 comprises four rotating shafts 9 arranged at the bottom of the stainless steel shell 1, a rotating motor 15 fixed in the base 2 and connected with the rotating shafts 9 in a one-to-one correspondence manner, and rotating speed adjusting knobs arranged on the outer side of the base 2, the temperature monitoring device 11 comprises a temperature display 11-1, a temperature setting button 11-2 and a temperature monitor 8 arranged in the water bag 3 and used for realizing constant temperature heating, more than one position holes 4 and two heating rods 7 are further arranged in the water bag 3, and water changing holes 6 extending out of the stainless steel shell 1 are formed in the side edge of the water bag 3.
Preferably, the base 2 is made of stainless steel and has a trapezoid cubic structure.
Preferably, the position holes 4 are respectively six 50ml centrifuge tube position holes with the caliber of 28.6mm and eight cryopreservation tube position holes with the caliber of 6.5mm, each position hole 4 is provided with a position hole heat preservation cover 5, and the aperture size of each position hole heat preservation cover 5 is consistent with that of the corresponding position hole 4.
Preferably, the base 2 is provided with a main switch 10.
Preferably, four balance support feet 14 are provided at the bottom of the base 2.
In this embodiment:
the inner wall of stainless steel shell 1 is provided with the heat preservation cotton, improves the heat preservation effect, and stainless steel shell 1 is cube no lid structure, and 4 axis of rotation 9 are connected to stainless steel shell 1 bottom, and stainless steel shell 1 bottom has three aperture, and wherein two apertures are used for pegging graft heating rod 7 and carry out the winding displacement, and one of them aperture is used for pegging graft temperature monitor 8 and carries out the winding displacement, and stainless steel shell 1 one side is provided with a water hole 6, and the function of stainless steel shell 1: the stainless steel shell 1 is mainly used for carrying a water bag, and the four rotating shafts rotate at the same speed, so that sterile water in the water bag is heated uniformly;
the base 2 is made of stainless steel, the structure of the base 2 is a trapezoid cubic structure, a heating rod control device, a temperature monitoring device, a rotary motor control device and a timing device are arranged in the base 2, 4 balance supporting feet 14 are arranged at the bottom of the base 2, and a switch button, a rotary shaft rotating speed adjusting knob, a temperature setting button and a timing button are arranged on the front surface of the base 2. The bottom of the base 2 is provided with 4 holes, so that the rotating shaft 9 can conveniently drive the stainless steel shell 1 to horizontally rotate, and the functions of the stainless steel shell 1 are used for accommodating various switches, supporting the water bag and stabilizing the stainless steel shell 1.
The water bag 3 is an insulating film with good thermal conductivity and a cubic structure, and is internally provided with 6 50ml centrifuge tube position holes with the caliber of 28.6mm and 8 freezing storage tube position holes with the caliber of 6.5 mm; 2 intelligent heating rods and 1 temperature monitor are arranged, and a water changing hole is formed in one side of the intelligent heating rod. The functions are as follows: sterile water is filled in the water bag 3, the sterile water is heated by 2 heating rods in the water bag 3, and the temperature monitor is kept constant, so that the heated sterile water is positioned in the biological sample in the position hole through heat conduction;
the material of the position hole 4 is an insulating film with good thermal conductivity, the structure is 6 position holes of 50ml centrifuge tube with caliber of 28.6mm, 8 position holes of freezing tube with caliber of 6.5mm, and the functions are as follows: the sample with proper specification is placed in the position hole, the temperature monitor is kept constant by heating the sample with proper specification through 2 heating rods in the water bag, so that the heated sterile water is positioned in the biological sample in the position hole through the heat conduction effect, the frozen storage tubes with different diameters can be realized, the centrifuge tube is used at the same time, the time is saved, the working efficiency of operators is improved, the position hole adopts a film with excellent heat conduction performance, and when the constant temperature water bag heating device is used, the film protects the sterile water medium combination material from being exposed in a clean environment, and bacteria are prevented from growing due to the fact that the sterile water is used as a pollution source, so that the constant temperature water bag heating device is very suitable for the requirement of culturing stem cells for a long time, and the film has excellent sealing performance. The water leakage and water seepage are avoided in the process of heating the water bag, so that the environment in the clean area is moist and is easy to breed a pollution source, and the film insulating material or the film insulating material can avoid electric shock to an operator in the process of heating the water bag in the operation experiment process, so that the health and safety of the operator are affected.
The heat-insulating cover 5 is made of heat-insulating cotton, and the structure of the heat-insulating cover is consistent with the pore diameter of the position hole. Functional action: the thermal insulation function is achieved, the biological sample in the protection position hole is uniformly heated, and the local temperature inconsistency is prevented;
the water changing hole 6 is made of colloid and has the structure: the nut is combined with a screw, and has the function effect that: the nut is rotated to remove the sterile water in the water bag, and the water bag is covered by rotation after being filled with the sterile water;
the material of heating rod 7 is intelligent heating device, and its structure can realize intelligent heating, keeps the water bag constant temperature, its function: the intelligent heating can be realized by acting together with the temperature monitoring device 11, and the water bag is kept at a constant temperature;
the temperature monitor 8 is an intelligent temperature monitoring device, and the structure of the temperature monitor can realize intelligent temperature monitoring, keep the water bag constant temperature and has the function of: the intelligent heating can be realized by acting together with the heating rod device, and the water bag is kept at a constant temperature.
The rotating shaft 9 is made of stainless steel, and has an inverted Z-shaped structure. One end is connected with the stainless steel shell 1, and one end is connected with the rotating motor 15, and the function of the rotating motor is as follows: the stainless steel shell 1 is driven to rotate and shake by converting the power of the rotary motor 15, so that the sterile water in the water bag is heated uniformly;
functional role of the master switch 10: the overall circuit switch of the device of the utility model is controlled.
The structure of the temperature monitoring device 11: consists of a temperature display, a temperature setting button and a temperature monitor, and has the function effect: can realize intelligent temperature monitoring and keep the water bag constant temperature.
The structure of the rotation speed adjusting device 12: comprises a rotating speed adjusting knob, a rotating motor and a rotating shaft. Functional action: controlling the rotation shaking frequency to drive the stainless steel shell 1, and driving the water bag by the stainless steel shell 1 to keep the sterile water in the water bag constant temperature;
the structure of the timer 13: consists of a timing display, a time setting button and a time alarm. Functional action: for monitoring the sample reaction time. The function of counting down can be realized, and an alarm prompt is given as soon as the time arrives; a time counting function;
material of the balance support leg 14: stainless steel support and plastic material. The structure is as follows: four stainless steel columns with threads and plastic spin feet. Functional action: the base 2 is balanced by rotating 4 plastic spin feet.
The function of the rotary motor 15: the stainless steel shell 1 is driven, and the stainless steel shell 1 drives the water bag to keep the sterile water in the water bag constant temperature.
The working principle of the structure is as follows: the cells are placed in the corresponding position holes 4, the stainless steel shell 1 is driven to swing by driving the rotating motor 15, meanwhile, the heating rod 7 is driven to heat, and the temperature is detected in real time by the temperature monitor 8 to realize constant-temperature heating.
In the embodiment, the heat insulation layer 16 is arranged between the stainless steel shell 1 and the water bag 3, so that the heat conduction and heat dissipation of the metal shell can be prevented, the temperature around the water bag is uneven, and the effect of constant temperature cannot be achieved.
The advantage of this structure is as follows:
1. the heat conducting medium in the water bag is sterile water: the medium has excellent heat conductivity and low cost, and can be replaced by new sterile water at any time;
2. sterile water in the water bag can be replaced at any time;
3. the base is provided with an oscillating device: when the operation is performed to recover cells, hands of an operator do not need to shake continuously in the sterile water medium, and the water bag bottom oscillating device of the device continuously oscillates the water bag in an electric control mode so as to keep stable heat transfer of the sterile water medium in the water bag, keep the medium in the water bag in a constant-temperature state, liberate the hands of the operator, and simultaneously avoid the condition that the operator is easy to scald the hands when using an open water bath for a long time;
4. setting a time timing device: the device has the functions of countdown and stopwatch, so that the distraction and error of operators in the operation process are reduced; meanwhile, time is saved, and working efficiency is improved;
5. the plurality of caliber holes are arranged, so that the freezing storage tubes with different diameters can be realized, and the centrifuge tubes are used at the same time, so that the time is saved, and the working efficiency of operators is improved;
6. the heating device is provided with an independent sealing water bag, and only the constant-temperature heating water bag is needed to complete heat transfer, so that water source or cell pollution is avoided;
7. the utility model adopts the sealed water bag, avoids the contact of thalli, does not form a fungus membrane layer and does not need cleaning.
The above-described embodiments are merely a few preferred embodiments of the present utility model, and many alternative modifications and combinations of the above-described embodiments will be apparent to those skilled in the art based on the technical solutions of the present utility model and the related teachings of the above-described embodiments.
Claims (5)
1. The utility model provides a cell experiment uses constant temperature water bag heating device, includes stainless steel shell (1) and base (2), its characterized in that: stainless steel shell (1) be cube no lid structure be provided with in stainless steel shell (1) water bag (3) that are cube structure, inside aseptic water that fills up of water bag (3) base (2) in be provided with rotational speed adjusting device (12), temperature monitoring device (11), timing device (13), rotational speed adjusting device (12) including setting up four axis of rotation (9) of stainless steel shell (1) bottom, fixed in base (2) and with axis of rotation (9) one-to-one be connected rotary motor (15) and be located the rotational speed adjust knob of base (2) outside, temperature monitoring device (11) including setting up temperature display (11-1) at base (2) front surface, temperature setting button (11-2) and setting up temperature monitor (8) that realize constant temperature heating in water bag (3), still be provided with more than one bit hole (4), two heating sticks (7) in water bag (3) side be provided with water bag (3) shell (1) that stretches out of water.
2. The cell assay of claim 1 using a thermostatic water bag heating device, wherein: the base (2) is made of stainless steel and is of a trapezoid cubic structure.
3. A cell assay according to claim 2 using a thermostatic water bag heating device, wherein: the position holes (4) are respectively six 50ml centrifuge tube position holes with the caliber of 28.6mm and eight cryopreservation tube position holes with the caliber of 6.5mm, each position hole (4) is provided with a position hole heat preservation cover (5), and the aperture size of each position hole heat preservation cover (5) is consistent with that of the corresponding position hole (4).
4. A cell assay according to claim 3 using a thermostatic water bag heating device, wherein: a main switch (10) is arranged on the base (2).
5. The device for heating a cell experiment using a constant temperature water bag according to claim 4, wherein: four balance supporting feet (14) are arranged at the bottom of the base (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320306418.XU CN219526642U (en) | 2023-02-23 | 2023-02-23 | Constant-temperature water bag heating device for cell experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320306418.XU CN219526642U (en) | 2023-02-23 | 2023-02-23 | Constant-temperature water bag heating device for cell experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219526642U true CN219526642U (en) | 2023-08-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320306418.XU Active CN219526642U (en) | 2023-02-23 | 2023-02-23 | Constant-temperature water bag heating device for cell experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219526642U (en) |
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- 2023-02-23 CN CN202320306418.XU patent/CN219526642U/en active Active
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