CN219496163U - Portable S100 protein chemiluminescence detection device - Google Patents
Portable S100 protein chemiluminescence detection device Download PDFInfo
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- CN219496163U CN219496163U CN202320235932.9U CN202320235932U CN219496163U CN 219496163 U CN219496163 U CN 219496163U CN 202320235932 U CN202320235932 U CN 202320235932U CN 219496163 U CN219496163 U CN 219496163U
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Abstract
The utility model discloses a portable S100 protein chemiluminescence detection device, which comprises: the detection device body comprises a liquid storage area, a reaction area and a collection area, wherein the liquid storage area is arranged above the reaction area, the liquid storage area comprises a liquid storage tank and an upper end switching plate arranged at the bottom of the liquid storage tank, and the upper end switching plate is used for controlling the liquid storage area to be communicated with or closed with the reaction area; the collecting area is arranged below the reaction area and comprises a collecting tank and a lower end switching plate arranged at the top of the collecting tank, and the lower end switching plate is used for controlling the reaction area to be communicated with or closed to the collecting area; the reaction zone comprises a fixed cylinder and a rotary cylinder nested in the fixed cylinder, a plurality of reaction balls are arranged in the rotary cylinder, a liquid flow inlet and a liquid flow outlet are arranged on the rotary cylinder, the liquid flow inlet can be connected with an upper end switching plate, and the liquid flow outlet can be connected with a lower end switching plate; the fixed cylinder is provided with a rotating motor, and the rotating motor can drive the rotating cylinder to rotate in the fixed cylinder when in operation.
Description
Technical Field
The utility model relates to the field of in-vitro diagnosis, in particular to a portable S100 protein chemiluminescence detection device.
Background
The human S100 protein is mainly concentrated in astrocytes and corresponding tumor cells of the central nervous system, and the S100 protein molecule consists of an alpha subunit and a beta subunit, has a molecular weight of 21000 and a biological half-life of 22h, and can be obtained by 100% dissolution in a saturated ammonium sulfate solution with a pH value of 7. The S100 protein in cerebrospinal fluid is age-related, and increases with age may be related to apoptosis of glial cells, demyelination, and metabolic changes of cerebrospinal fluid, but the concentration of S100 protein in plasma has no exact relation to age and sex. At present, the clinical specimen is detected by using an S100 protein monoclonal antibody, so that the tumor origin is identified, and the diagnosis accuracy of glioma is improved. The distribution of S100 protein is wider, and the S100 protein is mainly used for differential diagnosis of central nervous system tumors at present, and can be used as an important index for identifying whether the tumors are of ectodermal origin. Analysis of the expression of the S100 protein in 95 astrocytomas shows that each subtype of the S100 protein is expressed in astrocytomas and that the expression is inversely related to the malignancy of the tumor.
From the detection point of view, the current method for clinically measuring S-100 mainly comprises a chemiluminescence method, but the current luminous detection kit on the market has the defects of complicated operation flow and long detection time, so that a portable S100 protein chemiluminescence detection device is provided to solve the problems.
Disclosure of Invention
The utility model overcomes the defects of the prior art and provides a portable S100 protein chemiluminescence detection device.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a portable S100 protein chemiluminescent detection device comprising: the detection device comprises a detection device body, a detection device body and a control device, wherein the detection device body comprises a liquid storage area, a reaction area and a collection area, the liquid storage area is arranged above the reaction area, the liquid storage area comprises a liquid storage groove and an upper end switching plate arranged at the bottom of the liquid storage groove, and the upper end switching plate is used for controlling the liquid storage area to be communicated with or closed with the reaction area;
the collecting zone is arranged below the reaction zone and comprises a collecting tank and a lower end switching plate arranged at the top of the collecting tank, and the lower end switching plate is used for controlling the reaction zone to be communicated with or closed to the collecting zone;
the reaction zone comprises a fixed cylinder and a rotary cylinder nested in the fixed cylinder, a plurality of reaction balls are arranged in the rotary cylinder, a liquid flow inlet and a liquid flow outlet are arranged on the rotary cylinder, the liquid flow inlet can be connected with the upper end switching plate, and the liquid flow outlet can be connected with the lower end switching plate;
the fixed cylinder is provided with a rotating motor, and the rotating motor can drive the rotating cylinder to rotate in the fixed cylinder when in operation.
In a preferred embodiment of the present utility model, a baffle slot is disposed in the fixed cylinder, and both the liquid flow inlet and the liquid flow outlet are provided with baffles, and the baffles can rotate along the baffle slot to prevent the liquid in the rotary cylinder from flowing into the fixed cylinder.
In a preferred embodiment of the present utility model, the liquid inlet is provided with a liquid flow plate, the liquid flow plate is provided with a plurality of liquid flow holes, the upper end switching plate is provided with a plurality of upper diversion holes, and the upper diversion holes are staggered with the liquid flow holes.
In a preferred embodiment of the present utility model, the upper end switching plate is provided with a rotating rod, and the rotating rod can drive the upper end switching plate to rotate along the liquid flow plate, so that the upper diversion hole is communicated with the liquid flow hole.
In a preferred embodiment of the present utility model, the liquid outlet is provided with a liquid flow plate, the liquid flow plate is provided with a liquid flow hole, the lower end switching plate is provided with a plurality of lower diversion holes, and the liquid flow hole and the lower diversion holes are arranged in a staggered manner.
In a preferred embodiment of the present utility model, the lower end switching plate is provided with a rotating rod, and the rotating rod can drive the lower end switching plate to rotate along the liquid flow plate, so that the lower diversion hole is communicated with the liquid flow hole.
In a preferred embodiment of the present utility model, the detection device body further includes a liquid injection area, the liquid injection area includes a liquid injection groove and a sealing film disposed at a notch of the liquid injection groove, the liquid injection groove is adapted to the liquid storage groove in size, and the liquid injection groove can be inserted into the liquid storage groove.
In a preferred embodiment of the present utility model, a spike device is disposed in the liquid storage tank, and the spike device can puncture the sealing film.
In a preferred embodiment of the utility model, a handheld plate is arranged at the top of the liquid injection groove, and the diameter length of the handheld plate is larger than that of the liquid injection groove.
In a preferred embodiment of the utility model, the reaction balls are plastic beads, and the diameter of the plastic beads is 1-3 mu m.
The utility model solves the defects existing in the background technology, and has the following beneficial effects:
(1) According to the utility model, the detection device body is composed of the liquid storage area, the reaction area and the collection area which are sequentially arranged from top to bottom, the sample directly circulates in each area through gravity in the use process, the reaction area is simply and quickly arranged in a sleeve form, and the rotary drum arranged at the inner layer rotates along the fixed drum under the action of the rotary motor, so that the reaction efficiency of the sample and reactants in the reaction area is accelerated, the detection duration is greatly shortened, and the working efficiency is improved.
(2) According to the utility model, the upper end conversion plate is arranged between the liquid storage area and the reaction area, the sample injection into the reaction area is realized by rotating the conversion plate, or the sample injection is stopped, medical staff can inject different amounts of samples or reaction liquid according to the requirements, the lower end conversion plate is arranged between the reaction area and the collection area, after the reaction is finished, the reaction area and the collection area can be directly communicated through the conversion plate, so that the waste liquid can be directly collected, the liquid storage and the reaction are simply and conveniently carried out, the working efficiency is high, and meanwhile, the liquid storage and the reaction are arranged on the same device, so that the liquid storage and the reaction are integrated, and the liquid storage and the reaction are convenient to carry and detect at any time.
(3) The utility model is also provided with a liquid injection area, a sample or a reactant is packaged in the liquid injection groove, the liquid injection groove is reversely buckled in the liquid storage groove, the packaging film is poked through the spike device in the liquid storage groove, the reactant or the sample is directly injected into the liquid storage groove, the sample or the reactant is in a closed state in the detection process, the pollution of the external environment to the sample or the reactant is avoided, the inaccurate experimental result is caused, and meanwhile, the pollution to the external environment such as a device and a table top is avoided when the sample or the reactant is injected into the device and is scattered outside the device.
Drawings
The utility model is further described below with reference to the drawings and examples;
FIG. 1 is a perspective view of a detecting device according to a preferred embodiment of the present utility model;
FIG. 2 is an exploded view of a detection device according to a preferred embodiment of the present utility model;
FIG. 3 is a perspective view of a detection device of a preferred embodiment of the present utility model;
FIG. 4 is an exploded view of the reaction zone of a preferred embodiment of the present utility model;
fig. 5 is a perspective view of the upper/lower switching board according to the preferred embodiment of the present utility model.
In the figure: 1. a liquid storage area; 11. a liquid storage tank; 12. an upper switching board; 120. a rotating lever; 121. an upper deflector hole; 13. a spike device;
2. a reaction zone; 21. a fixed cylinder; 210. a motor switch; 22. a rotary drum; 220. a liquid flow inlet; 221. a liquid flow outlet; 2210. a flow plate; 222. a baffle; 23. a reaction ball;
3. a collection zone; 31. a collection tank; 32. a lower end switching plate; 320. a lower deflector hole;
4. a liquid injection area; 41. a liquid injection groove; 410. a hand-held plate; 42. and (5) sealing films.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
As shown in fig. 1-5, a portable S100 protein chemiluminescent detection device comprises: the detection device comprises a detection device body, wherein the detection device body comprises a liquid storage area 1, a reaction area 2 and a collection area 3, the liquid storage area 1 is arranged above the reaction area 2, the liquid storage area 1 comprises a liquid storage groove 11 and an upper end switching plate 12 arranged at the bottom of the liquid storage groove 11, and the upper end switching plate 12 is used for controlling the liquid storage area 1 to be communicated with or closed with the reaction area 2; the collecting zone 3 is arranged below the reaction zone 2, the collecting zone 3 comprises a collecting tank 31 and a lower end switching plate 32 arranged at the top of the collecting tank 31, and the lower end switching plate 32 is used for controlling the reaction zone 2 to be communicated with or closed with the collecting zone 3; the reaction zone 2 comprises a fixed cylinder 21 and a rotary cylinder 22 nested in the fixed cylinder 21, a plurality of reaction balls 23 are arranged in the rotary cylinder 22, the reaction balls 23 are plastic beads, the diameter of each plastic bead is 1-3 mu m, a liquid flow inlet 220 and a liquid flow outlet 221 are arranged on the rotary cylinder 22, the liquid flow inlet 220 can be connected with the upper-end switching plate 12, and the liquid flow outlet 221 can be connected with the lower-end switching plate 32; the fixed cylinder 21 is provided with a rotating motor, and the rotating motor can drive the rotating cylinder 22 to rotate in the fixed cylinder 21 when working.
Through the liquid storage area 1, reaction zone 2 and the collection district 3 that arrange in proper order from top to bottom make up into the detection device body, the sample passes through gravity in the use, directly circulates in each region, and is simple swift, sets up reaction zone 2 to the sleeve form simultaneously, sets up the rotary drum 22 at the inlayer under the rotating electrical machines effect follow fixed section of thick bamboo 21 is rotatory to accelerate the reaction efficiency of sample and reaction zone 2 internal reactant, greatly shortened the detection duration, improved work efficiency.
In a preferred embodiment of the present utility model, the detecting device body further includes a liquid injection area 4, the liquid injection area 4 includes a liquid injection groove 41 and a sealing film 42 disposed at a notch of the liquid injection groove 41, the liquid injection groove 41 is matched with the liquid storage groove 11 in size, the liquid injection groove 41 can be inserted into the liquid storage groove 11, a spike device 13 is disposed in the liquid storage groove 11, and the spike device 13 can puncture the sealing film 42.
Through encapsulating sample or reactant in annotating cistern 41, with annotating cistern 41 back-off in reservoir 11, stab device 13 through in the reservoir 11 and stab the encapsulation membrane, pour reactant or sample directly into reservoir 11, in testing process sample or reactant be in the closed state, avoid external environment to its pollution, cause experimental result inaccurate, avoid simultaneously with sample or reactant to pour into the device outside the device, cause pollution to external environment such as device and desktop.
The top of the liquid injection groove 41 is provided with a handheld plate 410, and the diameter length of the handheld plate 410 is larger than that of the liquid injection groove 41, so that the handheld liquid injection groove 41 is convenient to insert into the liquid storage groove 11.
As shown in fig. 3-5, a baffle 222 groove is formed in the fixed cylinder 21, both the liquid inlet 220 and the liquid outlet 221 are provided with baffles 222, the baffles 222 can rotate along the baffle 222 groove to prevent liquid in the rotary cylinder 22 from flowing into the fixed cylinder 21, the liquid inlet 220 is provided with a liquid flow plate 2210, a plurality of liquid flow holes are formed in the liquid flow plate 2210, a plurality of upper diversion holes 121 are formed in the upper end switching plate 12, the upper diversion holes 121 are arranged in a staggered manner with the liquid flow holes, a rotating rod 120 is arranged on the upper end switching plate 12, and the rotating rod 120 can drive the upper end switching plate 12 to rotate along the liquid flow plate 2210, so that the upper diversion holes 121 are communicated with the liquid flow holes. Through setting up the upper end conversion board between stock solution district 1 and reaction district 2, realize pouring into reaction district 2 with the sample through rotating the conversion board in, perhaps stop sample injection, medical personnel can pour into different volume samples or reaction liquid according to the demand
The liquid outlet 221 is provided with a liquid flow plate 2210, a plurality of liquid flow holes are formed in the liquid flow plate 2210, a plurality of lower flow guide holes 320 are formed in the lower end switching plate 32, the liquid flow holes are staggered with the lower flow guide holes 320, the lower end switching plate 32 is provided with a rotating rod 120, and the rotating rod 120 can drive the lower end switching plate 32 to rotate along the liquid flow plate 2210, so that the lower flow guide holes 320 are communicated with the liquid flow holes. The lower end conversion plate is arranged between the reaction zone 2 and the collection zone 3, after the reaction is finished, the reaction zone 2 and the collection zone 3 can be directly communicated through the switching plate, so that the waste liquid can be directly collected, the operation is simple and convenient, and the working efficiency is high.
In the use of the present utility model,
example 1: s100 protein chemiluminescence detection
10 microliters of a plasma sample (male sample, 26 years old) with sodium citrate anticoagulant was manually injected into the reservoir 1, and the plasma sample flowed downwardly into the reaction zone 2 through the lower flow-directing aperture 320 of the upper switching plate 12 due to the influence of gravity. The enzyme-labeled reagent is packaged into the liquid injection groove 41 and then reversely buckled into the liquid storage groove 11, the sealing film 42 at the notch of the liquid injection groove 41 is pierced by the spike device 13, and 20 microlitres of the enzyme-labeled reagent can flow downwards into the reaction groove through the upper diversion hole 121 of the upper end switching plate 12 due to the influence of gravity.
The motor switch 210 is turned on, the rotary motor is operated for 5 minutes, and the rotary drum 22 is continuously rotated during which the plasma sample reacts with the capture antibody on the plastic beads (pre-packed with 20. Mu.L of plastic bead antibody solution) and the detection antibody on the enzyme-labeled reagent (the rotary bar 120 on the upper/lower end switching plate 32 sets the reaction vessel to the closed state). After the cultivation is completed, the rotating rod 120 on the lower end switching plate 32 is rotated, so that the lower diversion hole 320 on the lower end switching plate 32 is communicated with the upper diversion hole of the liquid outlet flow plate 2210, the waste liquid flows into the waste liquid collecting tank 31 under the influence of gravity, and the plastic beads are reserved in the reaction tank due to the fact that the size of the plastic beads is larger than that of the diversion hole.
The rotating rod 120 on the lower end switching plate 32 is rotated to make the lower diversion hole 320 on the lower end switching plate 32 and the liquid outlet liquid flow plate 2210 staggered, the cleaning liquid is sealed in the liquid injection groove 41 and reversely buckled into the liquid storage groove 11, the sealing film 42 of the notch of the liquid storage groove 11 is pierced by the spike device 13, and 30 microlitres of cleaning liquid is influenced by gravity and flows downwards into the reaction groove through the upper diversion hole 121 of the upper end switching plate 12. The motor switch 210 is turned on, the rotary motor is operated for 1 minute, the rotary drum 22 is continuously rotated, and the washing step is performed. And after the cleaning is finished, repeating the waste liquid collecting step, and collecting the cleaning waste liquid.
The rotating rod 120 on the lower end switching plate 32 is rotated again, so that the lower diversion hole 320 on the lower end switching plate 32 is staggered with the liquid flow hole of the liquid flow plate 2210, the substrate is packaged into the liquid injection groove 41 and then reversely buckled into the liquid storage groove 11, the spike in the liquid storage groove 11 pierces the sealing film 42 of the notch of the liquid injection groove 41, and 45 microlitres of the substrate can flow downwards into the reaction groove through the upper diversion hole 121 of the upper end switching plate 12 due to the influence of gravity. The motor switch 210 is turned on, the rotary motor works for 2 minutes, the rotary cylinder 22 rotates continuously, the reaction step is carried out, detection can be carried out after the reaction is carried out for 300 seconds, the measured luminescence value is 15,268RLU, the concentration value obtained by substituting the measured luminescence value into a reaction curve is 28 mug/L, and the result is judged to be positive.
Example 2: incubation time and reaction efficiency analysis
Different incubation times were verified and the reaction efficiency was analyzed from the reaction results under the same flow. Experimental parameters: incubation times were 3 minutes, 5 minutes, 10 minutes. Fixed parameters: referring to the S100 protein chemiluminescence detection flow, quality control product 1 is taken as a sample for detection (containing S100 recombinant antigen/escherichia coli recombinant purified antigen, 0.05M Tris buffer, 4% bovine serum albumin, 0.05% Proclin300, and the target value is 0.1 mug/L). Experimental analysis: the reaction efficiency was analyzed as a result of the concentration test of quality control 1 (test value X μg/L, quality control 1 target value 0.1 μg/L, reaction efficiency=x/0.1×100%). Experimental results: incubation time is 3 minutes, the concentration of the S100 protein control 1 is 0.072 mug/L, and the reaction efficiency is 72%; incubation time 5 minutes, measured S100 protein control 1 concentration of 0.092 μg/L, reaction efficiency 92% (10% increase efficiency per unit time); incubation time was 10 minutes, and the concentration of S100 protein control 1 was measured to be 0.095. Mu.g/L, and the reaction efficiency was 95% (the increase efficiency per unit time was 0.6%). The results showed that the longer the incubation time, the higher the reaction efficiency. The final selection incubation time was 5 minutes in view of the increased efficiency per unit time.
The foregoing examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present utility model, which are equivalent to the above embodiments according to the essential technology of the present utility model, and these are all included in the protection scope of the present utility model.
Claims (10)
1. A portable S100 protein chemiluminescent detection device comprising: the detection device body is characterized in that,
the detection device body comprises a liquid storage area, a reaction area and a collection area, wherein the liquid storage area is arranged above the reaction area, the liquid storage area comprises a liquid storage tank and an upper end switching plate arranged at the bottom of the liquid storage tank, and the upper end switching plate is used for controlling the liquid storage area to be communicated with or closed to the reaction area;
the collecting zone is arranged below the reaction zone and comprises a collecting tank and a lower end switching plate arranged at the top of the collecting tank, and the lower end switching plate is used for controlling the reaction zone to be communicated with or closed to the collecting zone;
the reaction zone comprises a fixed cylinder and a rotary cylinder nested in the fixed cylinder, a plurality of reaction balls are arranged in the rotary cylinder, a liquid flow inlet and a liquid flow outlet are arranged on the rotary cylinder, the liquid flow inlet can be connected with the upper end switching plate, and the liquid flow outlet can be connected with the lower end switching plate;
the fixed cylinder is provided with a rotating motor, and the rotating motor can drive the rotating cylinder to rotate in the fixed cylinder when in operation.
2. The portable S100 protein chemiluminescent detection of claim 1 wherein: the fixed cylinder is internally provided with a baffle groove, the liquid flow inlet and the liquid flow outlet are both provided with baffles, and the baffles can rotate along the baffle groove to prevent liquid in the rotary cylinder from flowing into the fixed cylinder.
3. The portable S100 protein chemiluminescent detection of claim 1 wherein: the liquid flow inlet is provided with a liquid flow plate, a plurality of liquid flow holes are formed in the liquid flow plate, a plurality of upper diversion holes are formed in the upper end switching plate, and the upper diversion holes are staggered with the liquid flow holes.
4. A portable S100 protein chemiluminescent detection assembly of claim 3 wherein: the upper end switching plate is provided with a rotating rod, and the rotating rod can drive the upper end switching plate to rotate along the liquid flow plate, so that the upper diversion hole is communicated with the liquid flow hole.
5. The portable S100 protein chemiluminescent detection of claim 1 wherein: the liquid outlet is provided with a liquid flow plate, a plurality of liquid flow holes are formed in the liquid flow plate, a plurality of lower diversion holes are formed in the lower end switching plate, and the liquid flow holes are staggered with the lower diversion holes.
6. The portable S100 protein chemiluminescent detection of claim 5 wherein: the lower end switching plate is provided with a rotating rod, and the rotating rod can drive the lower end switching plate to rotate along the liquid flow plate, so that the lower diversion hole is communicated with the liquid flow hole.
7. The portable S100 protein chemiluminescent detection of claim 1 wherein: the detection device body further comprises a liquid injection area, the liquid injection area comprises a liquid injection groove and a sealing film arranged at a notch of the liquid injection groove, the liquid injection groove is matched with the liquid storage groove in size, and the liquid injection groove can be inserted into the liquid storage groove.
8. The portable S100 protein chemiluminescent detection of claim 7 wherein: the liquid storage tank is internally provided with a spike device, and the spike device can puncture the sealing film.
9. The portable S100 protein chemiluminescent detection of claim 7 wherein: the top of the liquid injection groove is provided with a handheld plate, and the diameter length of the handheld plate is larger than that of the liquid injection groove.
10. The portable S100 protein chemiluminescent detection of claim 1 wherein: the reaction ball is a plastic bead with the diameter of 1-3 mu m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320235932.9U CN219496163U (en) | 2023-02-16 | 2023-02-16 | Portable S100 protein chemiluminescence detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320235932.9U CN219496163U (en) | 2023-02-16 | 2023-02-16 | Portable S100 protein chemiluminescence detection device |
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| CN219496163U true CN219496163U (en) | 2023-08-08 |
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| CN202320235932.9U Active CN219496163U (en) | 2023-02-16 | 2023-02-16 | Portable S100 protein chemiluminescence detection device |
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- 2023-02-16 CN CN202320235932.9U patent/CN219496163U/en active Active
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