CN219475121U - Sample purifying device for QuEChERS method - Google Patents

Abstract

The utility model provides a sample purifying device for a QuEChERS method, which comprises an injection syringe, a discharging pipe arranged at the bottom end of the injection syringe, a pushing component arranged at the top end of the injection syringe, a sealing base, a sealing top cover and a filtering component, wherein the sealing top cover is arranged on the sealing top cover; the sealing base comprises a shell, a plug body fixedly arranged in the shell, and a plug hole arranged at the top of the plug body and used for plugging and plugging the discharge pipe; the bottom of the shell is arc-shaped, the top of the shell is open, and the top of the shell is detachably connected with the bottom end of the injection syringe through threads; the sealing top cover is detachably covered on the top end of the injection needle cylinder and is used for sealing the top end opening of the discharging pipe. According to the utility model, the extracted supernatant in the QuEChERS method can be subjected to continuous vortex and filtration, and centrifugal operation after vortex can be omitted, so that frequent liquid transfer is not needed, the consumption of consumable materials can be reduced, and the sample processing efficiency and the accuracy of experimental results are improved.

Description

Sample purifying device for QuEChERS method

Technical Field

The utility model belongs to the technical field of pretreatment devices for analysis of veterinary drug residues, and particularly relates to a sample purification device for a QuEChERS method.

Background

The QuEChERS method is an emerging sample purification method, which is an abbreviation of Quick, easy, cheap, effective, rugged, safe, has the characteristics of rapidness, simplicity, convenience, low cost, high efficiency, durability and safety, and has very wide application in the field of pesticide and veterinary drug residue detection.

The basic principle of the QuEChERS method is that after a homogenized sample is extracted by acetonitrile (or acidified acetonitrile), salting out and layering are carried out by adopting extraction salt, an ethylenediamine-N-Propylsilane (PSA) or other adsorbents such as octadecylsilane (C18) and the like are combined with most of interferents (organic acids, fatty acids, carbohydrates and the like) in a matrix by adopting a matrix dispersion extraction mechanism, and then impurities are removed by vortex, centrifugation and filtration, so that the aim of purification is fulfilled.

Currently, decontamination centrifuge tubes are the devices commonly used in the QuEChERS process. Generally, a purification filler is pre-stored in a purification centrifuge tube, and when the centrifugal tube type purification tube is used, the supernatant obtained by extraction is added into the purification centrifuge tube, then vortexed, centrifuged, and the supernatant is taken again and filtered for instrument analysis. However, in the above operation steps, the supernatant obtained by extraction needs to be subjected to vortex, centrifugation and filtration operations in different experimental containers in sequence, so that sample liquid is inevitably required to be transferred for multiple times in the treatment process, the consumption of consumable materials can be increased, and meanwhile, system errors can be generated due to multiple times of liquid transfer, so that the accuracy of experimental results is affected.

Disclosure of Invention

In order to solve the problems, the utility model provides a sample purifying device for the QuEChERS method, which can continuously vortex and filter extraction supernatant in the QuEChERS method, and can omit centrifugal operation after vortex, so that frequent liquid rotation is not needed, the consumption of consumables can be reduced, and the sample processing efficiency and the accuracy of experimental results are improved.

In order to achieve the above purpose, the technical scheme of the utility model is as follows:

the sample purifying device for the QuEChERS method comprises an injection syringe, a discharging pipe arranged at the bottom end of the injection syringe, and a pushing component arranged at the top end of the injection syringe, and is characterized by further comprising: the sealing base, the sealing top cover and the filtering component;

the sealing base comprises a shell, a plug body fixedly arranged in the shell, and a plug hole arranged at the top of the plug body and used for plugging and plugging the discharge pipe; the bottom of the shell is arc-shaped, the top of the shell is open, and the top of the shell is detachably connected with the bottom end of the injection syringe through threads;

the sealing top cover is detachably covered on the top end of the injection needle cylinder and is used for sealing the top end opening of the discharging pipe;

the filtering component comprises a sieve plate fixedly arranged at the bottom of the syringe barrel and a purifying filler filled in the syringe barrel.

As a specific technical scheme, the bottom of the plug hole is in an upward protruding arc shape.

As a specific technical scheme, the shell is made of corrosion-resistant hard plastic, and the plug body is made of rubber.

As a specific technical scheme, the purifying filler is one or more of ethylenediamine-N-propyl silane, C18, graphitized carbon black, anhydrous magnesium sulfate and neutral aluminum oxide.

As a specific technical scheme, the upper end face of the sealing top cover is provided with a pinching handle.

As a specific technical scheme, one side of the sealing top cover is connected with the side surface of the top of the injection syringe through a pull rope.

As a specific technical scheme, the sieve pore diameter of the sieve plate is smaller than the particle size of the purifying filler.

Compared with the prior art, the utility model has the following beneficial effects:

1) The purification device can be used for continuously swirling and filtering the extracted supernatant in the QuEChERS method, and the centrifugal operation after swirling can be omitted, so that the sample liquid does not need to be frequently transferred in different containers, the experimental operation steps can be simplified, the experimental effect can be improved, meanwhile, the loss of a sample in the transfer process can be effectively reduced, the system error can be reduced, and the experimental accuracy can be improved.

2) The purifying device has the advantages of simple structure, convenient use, repeated use, reduced consumption of consumable materials and good popularization and practical value.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a front view of the present utility model;

FIG. 3 is a schematic view of the structure of the seal cap with the pushing assembly removed and the seal cap closed when the utility model is used;

FIG. 4 is a schematic view of a seal base according to the present utility model;

FIG. 5 is a schematic diagram of the present utility model when the sample solution is filtered;

FIG. 6 is a schematic illustration of the sample fluid being vortexed using the present utility model;

the meaning of each mark in the above figures is: syringe 1, discharging pipe 2, pushing component 3, sealing base 4, casing 401, plug 402, plug hole 403, sealing top cover 5, pinching handle 501, pulling rope 502, filtering component 6, sieve plate 601, purifying filler 602, sample liquid 7, filter membrane filter 8, vortex oscillator 9.

Detailed Description

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Examples

Referring to fig. 1 and 2, a sample purifying device for the QuEChERS method includes an injection syringe 1, a discharge tube 2 disposed at the bottom end of the injection syringe 1, a pushing assembly 3 disposed at the top end of the injection syringe 1, a sealing base 4, a sealing top cover 5, and a filtering assembly 6;

the injection needle cylinder 1 is a cylinder body with a cavity inside, the lower end of the cylinder body is closed, and the upper end of the cylinder body is open so as to be used for containing sample liquid; the pushing component 3 comprises a piston which is arranged in the injection needle cylinder 1 in a sliding manner, a pushing handle and a piston push rod which is connected between the piston and the pushing handle; the sealing base 4 comprises a shell 401, a plug body 402 fixedly arranged in the shell 401, and a plug hole 403 arranged at the top of the plug body 402 and used for plugging the discharge pipe 2; the bottom of the shell 401 is arc-shaped, the top of the shell is open, and the top of the shell 401 is detachably connected with the bottom end of the injection needle cylinder 1 through threads; when the shell 401 is screwed, the lower end of the discharging pipe 2 is inserted into the plug hole 403 and is further abutted against the bottom of the plug hole 403 to be plugged, so that the sample liquid can be prevented from leaking out of the discharging pipe 2; the sealing top cover 5 is detachably covered on the top end of the injection needle cylinder 1 and is used for sealing the top end opening of the discharging pipe 2; the filtering component 6 comprises a sieve plate 601 fixedly arranged at the bottom of the cylinder body of the injection cylinder 1, and a purifying filler 602 filled in the cylinder body of the injection cylinder 1; in the filtering component 6, the purifying filler 602 is used for adsorbing impurities in the sample liquid, which interfere with detection; the sieve plate 601 is used for filtering larger particles in the liquid to be tested and blocking the purification packing 602 so as to prevent the purification packing 602 from leaking out of the discharge pipe 2;

the application method or the working principle of the utility model is as follows: referring to fig. 3, when pretreatment is performed on a sample solution by the QuEChERS method, the pushing component 3 of the device of the present utility model should be pulled out from the upper end of the syringe 1, the extracted supernatant is added into the syringe 1, and then the sealing top cover 5 is covered, so that the supernatant is sealed and contained in the syringe 1; referring to fig. 6, the device of the present utility model is then placed obliquely on the vortex oscillator 9, such that the bottom end of the housing 401 of the seal base 4 is abutted against the oscillation disk of the vortex oscillator 9, and then the vortex oscillator 9 is turned on, such that the supernatant is in full mixed contact with the purification packing 602; the bottom of the shell 401 is arc-shaped, so that the supporting inclination angle of the injection needle cylinder 1 on the oscillating disc and the contact stress degree of the injection needle cylinder 1 and the oscillating disc can be conveniently adjusted; in addition, the shell 401 is in threaded connection with the injection needle cylinder 1, so that the sealing base 4 can be firmly installed, the sealing base 4 is prevented from falling off or loosening in the vortex oscillation process to influence the blocking effect on the discharge pipe 2, and the sealing base 4 can be conveniently detached when needed; referring to fig. 5, after the vortex oscillation of the supernatant is completed, the seal top cover 5 is removed, then the pushing component 3 is installed in the syringe 1, and then the seal base 4 is unscrewed; then pressing the pushing component 3, filtering the supernatant fluid after vortex oscillation through the sieve plate 601 under a certain pressure, intercepting the purifying filler 602 and impurities and interferents adsorbed by the purifying filler 602 by the sieve plate 601, and finally discharging the supernatant fluid from the outlet end of the discharging pipe 2 to realize the purification of the supernatant fluid; at this time, a filter membrane filter 8 may be further connected to the outlet end of the discharge pipe 2, so as to directly filter the filtered supernatant with a filter membrane, and then perform subsequent measurement by loading.

Further, in a preferred embodiment, referring to fig. 4, the bottom of the hole 403 is in an arc shape protruding upwards, so that when the tapping pipe 2 is inserted into the bottom of the hole 403, the outlet end of the tapping pipe 2 can be better plugged to prevent leakage.

Further, in a preferred embodiment, the housing 401 is made of a hard plastic that is corrosion resistant and the plug body 402 is made of rubber.

Further, in a preferred embodiment, the purge filler 602 is one or more of ethylenediamine-N-propylsilane, C18, graphitized carbon black, anhydrous magnesium sulfate, neutral alumina.

To facilitate opening and closing of the seal cap 5, further, in a preferred embodiment, referring to fig. 1, the seal cap 5 is provided with a pinching grip 501 at its upper end face.

Further, in a preferred embodiment, referring to fig. 1 and 3, one side of the seal cap 5 is connected to the top side of the syringe 1 by a pull cord 502, so that the opened seal cap 5 can be suspended by the pull cord 502 to prevent the seal cap 5 from being lost.

Further, in a preferred embodiment, the mesh openings of the screen 601 are smaller than the particle size of the purge filler 602.

Claims (7)

1. Sample purification device for QuEChERS method, including syringe (1), set up discharging pipe (2) in syringe (1) bottom, set up pushing away material subassembly (3) on syringe (1) top, its characterized in that still includes: a sealing base (4), a sealing top cover (5) and a filtering component (6);

the sealing base (4) comprises a shell (401), a plug body (402) fixedly arranged in the shell (401), and a plug hole (403) arranged at the top of the plug body (402) and used for plugging the discharge pipe (2); the bottom of the shell (401) is arc-shaped, the top of the shell is open, and the top of the shell (401) is detachably connected with the bottom end of the injection needle cylinder (1) through threads;

the sealing top cover (5) is detachably covered on the top end of the injection needle cylinder (1) and is used for sealing the top end opening of the discharging pipe (2);

the filtering component (6) comprises a sieve plate (601) fixedly arranged at the bottom of the cylinder body of the injection cylinder (1), and a purifying filler (602) filled in the cylinder body of the injection cylinder (1).

2. Sample purification device for use in the QuEChERS method according to claim 1, characterised in that the bottom of the plug hole (403) is in the shape of an upwardly protruding arc.

3. The sample purification device for use in the QuEChERS method according to claim 1, characterised in that the housing (401) is made of a hard plastic resistant to corrosion and the plug body (402) is made of rubber.

4. The sample purification apparatus for use in the QuEChERS process of claim 1, wherein the purification filler (602) is one or more of ethylenediamine-N-propylsilane, C18, graphitized carbon black, anhydrous magnesium sulfate, neutral alumina.

5. Sample purification device for use in the QuEChERS method according to claim 1, characterised in that the upper end face of the sealing cap (5) is provided with a pinching grip (501).

6. Sample purification device for use in the QuEChERS method according to claim 1, characterised in that one side of the seal cap (5) is connected to the top side of the syringe (1) by a pull cord (502).

7. Sample purification device for use in a QuEChERS process according to any of claims 1-6, characterised in that the sieve plate (601) has a sieve pore size smaller than the particle size of the purification packing (602).