CN219330460U

CN219330460U - Nematode hatching device

Info

Publication number: CN219330460U
Application number: CN202320513681.6U
Authority: CN
Inventors: 顾沛雯; 裴伟; 范芮甫
Original assignee: Ningxia University
Current assignee: Ningxia University
Priority date: 2023-03-16
Filing date: 2023-03-16
Publication date: 2023-07-14
Anticipated expiration: 2033-03-16

Abstract

The utility model belongs to the technical field of nematode hatching devices, and particularly relates to a nematode hatching device, which comprises a box body, wherein a box cover is fastened at the top end of the box body, a fixed plate is fixedly connected to the upper part inside the box body, a plurality of hatching mechanisms are vertically arranged on the fixed plate in a sliding manner, the hatching mechanisms are arranged at equal intervals, a plurality of culture solution guiding mechanisms are fixedly connected to the lower part inside the box body, the culture solution guiding mechanisms are in one-to-one correspondence with the hatching mechanisms, the bottom ends of the hatching mechanisms are vertically and slidably connected with the culture solution guiding mechanisms, an observation dish is correspondingly arranged at the bottom ends of the culture solution guiding mechanisms, and an object taking hole is formed at the bottom end of one side wall of the box body. The device can extract and observe the culture solution at any time to determine the hatching progress of the root-knot nematodes, and is convenient to use.

Description

Nematode hatching device

Technical Field

The utility model belongs to the technical field of nematode hatching devices, and particularly relates to a nematode hatching device.

Background

Root knot nematodes are serious plant pathogenic nematodes, and are of various types, strong in pathogenicity and wide in host range, and are distributed all over the world. The life history of the root knot nematode includes three stages, eggs, larvae and adults, with the second-instar larvae being the only larvae with infestations. After the second-instar larvae infest the plants, a large number of root knots are formed at the roots of the plants, and the absorption and utilization of nutrient substances such as nutrients, moisture and the like by the plants are seriously affected. The method can effectively prevent and treat the root knot nematode, and reduce the harm of the root knot nematode, and a large number of larvae are needed in the screening and control mode, so that the larvae need to be hatched and collected. In the process of hatching and collecting the larvae, the hatching progress of the larvae needs to be recorded by extracting the culture solution at any time for observation, and the process of extracting the culture solution by the hatching device in the prior art is complicated, so that the nematode hatching device needs to be designed to solve the problems.

Disclosure of Invention

The utility model aims to provide a nematode hatching device which solves the problems and achieves the aim of extracting and observing culture solution at any time.

In order to achieve the above object, the present utility model provides the following solutions: the utility model provides a nematode hatching apparatus, includes the box, box top lock joint has the case lid, the inside top fixedly connected with fixed plate of box, vertical slip is provided with a plurality of hatching mechanisms on the fixed plate, a plurality of hatching mechanism equidistant setting, the inside below fixedly connected with of box a plurality of culture solution lead-out mechanism, culture solution lead-out mechanism with hatching mechanism one-to-one, the hatching mechanism bottom with the vertical sliding connection of culture solution lead-out mechanism, culture solution lead-out mechanism bottom corresponds and is provided with the observation dish, a thing hole has been seted up to box side wall bottom.

Preferably, the hatching mechanism comprises a hatching barrel, the hatching barrel is vertically arranged on the fixed plate in a sliding manner, the bottom end of the hatching barrel is vertically connected with the culture solution guiding-out mechanism in a sliding manner, a screen is coaxially arranged in the hatching barrel, and the top end of the hatching barrel is fixedly connected with the top end of the screen through a fixing part.

Preferably, the fixing part comprises a fixing connecting rod, a group of outer clamping parts are fixedly connected to the bottoms of two ends of the fixing connecting rod respectively, each group of outer clamping parts comprises two outer clamping claws, the top end of the side wall of the hatching barrel is clamped between the two outer clamping claws, two groups of inner clamping parts are arranged between the outer clamping parts, each group of inner clamping parts comprises two inner clamping claws, and the top end of the side wall of the screen is clamped between the two inner clamping claws.

Preferably, the culture solution guiding-out mechanism comprises a fixed base, the fixed base with lateral wall fixed connection in the box, diapire middle part fixedly connected with stopper in the fixed base, the stopper both sides are provided with the spring respectively, the spring top with hatching bucket bottom fixed connection, the spring bottom with diapire fixed connection in the fixed base, the second through-hole has been seted up to the fixed base lateral wall, the second through-hole intercommunication has catheter one end, the catheter with fixed base lateral wall fixed connection, the catheter other end is located observe the dish directly over.

Preferably, a first through hole is formed in the bottom of the side wall of the hatching barrel, the first through hole corresponds to the second through hole, a sealing block is fixedly connected to the inner side wall of the fixed base and located above the second through hole, and the sealing block is in sealing contact with the bottom of the outer side wall of the hatching barrel.

Preferably, handles are fixedly connected to the middle parts of the two opposite outer side walls of the box body respectively.

Compared with the prior art, the utility model has the following advantages and technical effects:

according to the utility model, the larvae can be cultivated and hatched through the hatching mechanism, the culture solution can be guided out at any time through the culture solution guiding mechanism in the process of cultivation and hatching, and guided into the observation dish, and then the observation dish containing the culture solution can be taken out through the object taking hole arranged at the bottom end of the side wall of the box body and placed under a microscope for observation, so that the cultivation and hatching progress of the nematodes can be determined, and the device is very convenient to use.

Drawings

For a clearer description of an embodiment of the utility model or of the solutions of the prior art, the drawings that are needed in the embodiment will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art:

FIG. 1 is a schematic diagram of the overall structure of the device of the present utility model;

FIG. 2 is a schematic view of the internal structure of the device of the present utility model;

FIG. 3 is an enlarged view of a portion of FIG. 2A;

fig. 4 is a partial enlarged view of B in fig. 2.

Wherein, 1, the box body; 2. a case cover; 3. a fixing plate; 4. hatching barrel; 5. a screen; 6. a fixed connecting rod; 7. a fixed base; 8. a catheter; 9. an observation dish; 10. a spring; 11. a limiting block; 12. a sealing block; 13. a first through hole; 14. a second through hole; 15. an outer jaw; 16. an inner jaw; 17. a fetching hole; 18. a handle.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In order that the above-recited objects, features and advantages of the present utility model will become more readily apparent, a more particular description of the utility model will be rendered by reference to the appended drawings and appended detailed description.

Referring to fig. 1-4, the utility model provides a nematode hatching device, which comprises a box body 1, wherein the top end of the box body 1 is buckled with a box cover 2, a fixed plate 3 is fixedly connected to the upper part inside the box body 1, a plurality of hatching mechanisms are vertically and slidably arranged on the fixed plate 3, the hatching mechanisms are arranged at equal intervals, a plurality of culture solution guiding mechanisms are fixedly connected to the lower part inside the box body 1, the culture solution guiding mechanisms are in one-to-one correspondence with the hatching mechanisms, the bottom ends of the hatching mechanisms are vertically and slidably connected with the culture solution guiding mechanisms, the bottom ends of the culture solution guiding mechanisms are correspondingly provided with observation dishes 9, and a fetching hole 17 is formed in the bottom end of one side wall of the box body 1.

The hatching mechanism that sets up can cultivate the hatching to the larva, cultivates the in-process of hatching and can export the culture solution at any time through the culture solution guiding out mechanism that sets up, exports it in the observation dish 9, later can take out the observation dish 9 that holds the culture solution through the thing hole 17 of getting of seting up of box 1 lateral wall bottom and put under the microscope and observe to confirm the cultivation incubation progress of nematode, the device is very convenient to use.

Further optimizing scheme, hatching mechanism includes hatching bucket 4, and hatching bucket 4 vertical sliding arrangement is on fixed plate 3, and hatching bucket 4 bottom and the vertical sliding connection of culture solution guide-out mechanism, and hatching bucket 4 inside is coaxial to be provided with screen cloth 5, and hatching bucket 4 top passes through fixed part fixed connection with screen cloth 5 top.

Firstly, the box cover 2 is opened, egg blocks of the root-knot nematodes are placed in the screen 5, the screen 5 is 300 meshes, the screen 5 is fixed in the hatching barrel 4 through the fixing part, distilled water is added into the hatching barrel 4, the added water is slightly higher than the bottom of the screen 5, then the box body 1 is placed in a culture environment at 25-28 ℃ for hatching, and after hatching is completed, the root-knot nematodes can enter water under the action of water-logging. The larvae have a size of 1-1.5mm and a diameter of 0.03-0.05mm, and are very small, so that the larvae can enter water through the meshes of the screen 5, while the egg masses are gathered together by a plurality of eggs, and the size is far greater than that of the larvae, so that the egg masses can stay on the screen 5 before hatching into the larvae.

Further optimizing scheme, fixed part includes fixed connecting rod 6, and fixed connecting rod 6 both ends bottom is a set of outer clamping part of fixedly connected with respectively, and every outer clamping part of group includes two outer clamping claws 15, and incubation bucket 4 lateral wall top joint is between two outer clamping claws 15, is provided with two sets of interior clamping parts between two outer clamping parts of group, and every interior clamping part of group includes two interior clamping claws 16, screen cloth 5 lateral wall top joint between two interior clamping claws 16.

When the hatching barrel 4 and the screen 5 are fixed, the screen 5 is fixed on the fixed connecting rod 6 through the inner claw 16, then the screen 5 is placed in the hatching barrel 4, and the fixed connecting rod 6 is fixed on the top end of the side wall of the hatching barrel 4 through the outer claw 15, so that the relative fixation of the hatching barrel 4 and the screen 5 can be completed.

Further optimizing scheme, culture solution guiding mechanism includes unable adjustment base 7, unable adjustment base 7 and box 1 inside wall fixed connection, unable adjustment base 7 inner bottom middle part fixedly connected with stopper 11, stopper 11 both sides are provided with spring 10 respectively, spring 10 top and hatching bucket 4 bottom fixed connection, spring 10 bottom and unable adjustment base 7 inner bottom wall fixed connection, the second through-hole 14 has been seted up to unable adjustment base 7 lateral wall, second through-hole 14 intercommunication has catheter 8 one end, catheter 8 and unable adjustment base 7 lateral wall fixed connection, the catheter 8 other end is located directly over the observation dish 9.

According to a further optimization scheme, the bottom of the side wall of the hatching barrel 4 is provided with a first through hole 13, the first through hole 13 corresponds to a second through hole 14, the inner side wall of the fixed base 7 is fixedly connected with a sealing block 12, the sealing block 12 is located above the second through hole 14, and the sealing block 12 is in sealing contact with the bottom of the outer side wall of the hatching barrel 4.

When the hatching progress of the root-knot nematode needs to be determined, the box cover 2 is opened, the fixed connecting rod 6 is pressed downwards, the hatching barrel 4 and the screen 5 can be driven to integrally move downwards, when the hatching barrel 4 bottom is moved to be in contact with the limiting block 11, the first through hole 13 is aligned with the second through hole 14, the culture solution in the hatching barrel 4 is led out along the liquid guide tube 8 and finally flows into the observation dish 9, then the fixed connecting rod 6 is loosened, under the action of the reset elastic force of the spring 10, the hatching barrel 4 and the screen 5 integrally move upwards to reset, the first through hole 13 and the second through hole 14 are staggered after reset, the sealing block 12 seals the first through hole 13, the culture solution does not flow out any more, and then the observation dish 9 containing the culture solution is taken out through the object taking hole 17 to be observed under a microscope.

Further optimizing scheme, the middle parts of the two opposite outer side walls of the box body 1 are respectively fixedly connected with handles 18.

The handle 18 facilitates the movement of the device as a whole.

In the description of the present utility model, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present utility model.

The above embodiments are only illustrative of the preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model, and various modifications and improvements made by those skilled in the art to the technical solutions of the present utility model should fall within the protection scope defined by the claims of the present utility model without departing from the design spirit of the present utility model.

Claims

1. The utility model provides a nematode hatching apparatus, its characterized in that, includes box (1), box (1) top lock joint has case lid (2), inside top fixedly connected with fixed plate (3) of box (1), vertical sliding is provided with a plurality of hatching mechanisms on fixed plate (3), a plurality of hatching mechanism equidistant setting, the inside below fixedly connected with of box (1) a plurality of culture solution exports the mechanism, culture solution export the mechanism with hatching mechanism one-to-one, hatching mechanism bottom with culture solution export mechanism vertical sliding connection, culture solution exports the mechanism bottom and corresponds and be provided with observation dish (9), a thing hole (17) has been seted up to box (1) a side wall bottom.

2. The nematode hatching device according to claim 1, wherein the hatching mechanism comprises a hatching barrel (4), the hatching barrel (4) is vertically and slidably arranged on the fixed plate (3), the bottom end of the hatching barrel (4) is vertically and slidably connected with the culture solution guiding-out mechanism, a screen (5) is coaxially arranged in the hatching barrel (4), and the top end of the hatching barrel (4) is fixedly connected with the top end of the screen (5) through a fixed part.

3. A nematode hatching device according to claim 2, wherein the fixing portion comprises a fixing connecting rod (6), a group of outer clamping portions are fixedly connected to bottoms of two ends of the fixing connecting rod (6), each group of outer clamping portions comprises two outer clamping claws (15), the top end of the side wall of the hatching barrel (4) is clamped between the two outer clamping claws (15), two groups of inner clamping portions are arranged between the two groups of outer clamping portions, each group of inner clamping portions comprises two inner clamping claws (16), and the top end of the side wall of the screen (5) is clamped between the two inner clamping claws (16).

4. The nematode hatching device according to claim 2, wherein the culture fluid guiding-out mechanism comprises a fixed base (7), the fixed base (7) is fixedly connected with the inner side wall of the box body (1), a limiting block (11) is fixedly connected with the middle part of the inner side wall of the fixed base (7), springs (10) are respectively arranged on two sides of the limiting block (11), the top ends of the springs (10) are fixedly connected with the bottom end of the hatching barrel (4), the bottom ends of the springs (10) are fixedly connected with the inner side wall of the fixed base (7), a second through hole (14) is formed in the side wall of the fixed base (7), one end of a liquid guide tube (8) is communicated with the outer side wall of the fixed base (7), and the other end of the liquid guide tube (8) is located right above the observation dish (9).

5. The nematode hatching device according to claim 4, wherein a first through hole (13) is formed in the bottom of the side wall of the hatching barrel (4), the first through hole (13) corresponds to the second through hole (14), a sealing block (12) is fixedly connected to the inner side wall of the fixed base (7), the sealing block (12) is located above the second through hole (14), and the sealing block (12) is in sealing contact with the bottom of the outer side wall of the hatching barrel (4).

6. A nematode hatching apparatus according to claim 1, wherein handles (18) are fixedly connected to the middle portions of the two opposite outer side walls of the casing (1), respectively.