CN216088376U - Part for collecting root-knot nematode second-instar larvae and device comprising same - Google Patents
Part for collecting root-knot nematode second-instar larvae and device comprising same Download PDFInfo
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- CN216088376U CN216088376U CN202121966320.4U CN202121966320U CN216088376U CN 216088376 U CN216088376 U CN 216088376U CN 202121966320 U CN202121966320 U CN 202121966320U CN 216088376 U CN216088376 U CN 216088376U
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Abstract
The utility model discloses a part for collecting second-instar larvae of root-knot nematodes, which comprises: a mesh screen having a first side for housing root knot nematode second instar larva egg masses and a second side opposite the first side; the supporting body is provided with through holes which penetrate through the upper surface and the lower surface of the supporting body and are matched with the shape of the mesh screen, and the side surfaces of the mesh screen are abutted against the inner side surfaces of the corresponding through holes so that the mesh screen can be detachably and fixedly arranged in the through holes; the culture dish is arranged in an opening manner and is provided with a culture chamber, and the support body is placed on the bottom surface of the culture dish; a first space is formed by a space surrounded by the second surface of the mesh screen, a part of the bottom surface of the culture dish opposite to the second surface and a part of the inner side surface of the support body sandwiched between the two surfaces, and the first space is communicated with the culture chamber.
Description
Technical Field
The utility model relates to a device for separating and collecting root-knot nematodes and second-instar larvae thereof, in particular to a separation and collection device which can separate and collect the root-knot nematodes in soil and can collect the second-instar larvae of the root-knot nematodes.
Background
Root knot nematode disease is a worldwide plant infectious disease caused by the pathogenic root knot nematode (melodogyne spp.) that has been more harmful than bacteria and viruses than pathogenic fungi. According to incomplete statistics, the economic loss of crops caused by root-knot nematodes in the world is about more than 1000 billion dollars every year, the average annual yield of agriculture in the world can be reduced by 10%, the economic loss of greenhouse vegetables in China is up to 4 billion dollars every year, and the production of vegetables and food and economic crops is seriously damaged.
The cultivation of improved varieties for resisting the root knot nematode disease is the most economic, safe and efficient measure for reducing and avoiding the disease loss, the efficient selection of disease-resistant genes is realized through the phenotype of plants after artificial inoculation of the root knot nematode, and the separation of the second-instar larvae of the root knot nematode with enough number and certain inoculation concentration is the basis for the phenotypic identification of the root knot nematode resistance.
At present, the root-knot nematode separating device generally has the problem of not simple and convenient enough operation and can not well meet the use requirements.
Therefore, the inventor considers that a separation and collection device which can separate and collect the root-knot nematode in the soil and can collect the second-instar larvae of the root-knot nematode is necessary.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved by the utility model is to provide a device for separating and collecting root-knot nematodes, which has the characteristics of simple structure, convenient use, high efficiency and the like, and can separate the root-knot nematodes in soil and collect second-instar larvae of the root-knot nematodes.
In order to solve the technical problems, the utility model adopts the technical scheme that:
a component for collecting second instar larvae of root knot nematodes, said component comprising:
a mesh screen having a first side for housing root knot nematode second instar larva egg masses and a second side opposite the first side;
the supporting body is provided with a through hole which penetrates through the upper surface and the lower surface of the supporting body and is matched with the shape of the mesh screen, and the side surface of the mesh screen is abutted against the inner side surface of the corresponding through hole so that the mesh screen can be detachably and fixedly arranged in the through hole;
the culture dish is arranged in an opening manner and is provided with a culture chamber, and the support body is placed on the bottom surface of the culture dish;
a first space is formed by a space surrounded by the second surface of the mesh screen, a part of the bottom surface of the culture dish opposite to the second surface, and a part of the inner side surface of the support body sandwiched between the two surfaces, and the first space is communicated with the culture chamber.
Preferably, the support body includes:
a steel ring having a circular through hole;
the upper ends of the support rods are fixed on the lower surface of the steel ring at intervals, and the lower ends of the support rods are placed on the bottom surface of the culture dish;
the first space is communicated with the culture chamber through gaps among the support rods.
Preferably, the mesh screen is arranged at the middle position of the through hole along the up-down direction, and an egg mass placing space is defined by the first surface of the mesh screen and the part of the inner side surface of the steel ring, facing upwards, of the first surface of the mesh screen, and is used for placing egg masses.
Preferably, the number of the supporting rods is three, and the supporting rods are arranged in an equilateral triangle.
Preferably, the support rods have a diameter of 2mm and a height of 2 mm.
Preferably, the steel ring is formed by embedding an inner steel ring and an outer steel ring, the height of the steel ring is 8mm, and the wall thickness of the steel ring is 2 mm.
Preferably, the mesh screen has a diameter of 50mm and a mesh number of 50 to 500 mesh.
Preferably, the mesh number of the mesh screen is 200 meshes.
The part for collecting the second-instar larvae of the root-knot nematodes provided by the utility model has the following technical effects:
set up the mesh screen, can filter some impurity, make the second age larva that moves about downwards through the mesh screen after the egg piece hatching clean, can be directly reserve to do the experiment, and first space and cultivation cavity intercommunication, can make the nematode pass through the mesh screen after, leave this first space, move about to other positions of cultivateing the cavity, do not confine to and move about in first space, the scope of moving about is big, dissolved oxygen is good, the nematode is active good, the nematode is very suitable for reserve experiment, and set up through this intercommunication, make and all have moving about of second age larva in the cultivation cavity, when collecting, can directly take out from cultivateing the cavity internal absorption, convenient collection.
And, the hatching process is probably as follows: placing the support body with the mesh screen into a culture dish, injecting a proper amount of clear water until the support body just submerges the mesh screen, picking a proper amount of root-knot nematode eggs in the mesh screen, waiting for the second-instar larvae of the root-knot nematodes to hatch for 2-7 days, and then collecting the second-instar larvae of the root-knot nematodes for experimental needs. According to the utility model, the mesh screen is arranged at the middle position, and the placing space is arranged, so that impurities in the egg mass submerged by water can be located in the placing space as much as possible, and the nematodes collected at the later stage are cleaner.
The present invention also provides an apparatus comprising a part for collecting second-instar larvae of root-knot nematodes, which is the above-mentioned part for collecting second-instar larvae of root-knot nematodes, the apparatus further comprising:
the base is provided with a plurality of grooves for accommodating the corresponding parts for collecting the second-instar larvae of the root-knot nematodes;
the at least three struts are fixedly arranged on the periphery of the base and extend upwards;
the funnel supporting plate is arranged in parallel to the base and is provided with a plurality of funnel embedding holes penetrating through the upper surface and the lower surface of the funnel supporting plate, and the funnel embedding holes are in one-to-one correspondence to the grooves in the base;
the small-diameter ends of the funnels penetrate through the funnel embedding holes and face the corresponding grooves in the direction, and the small-diameter ends are provided with switches of the funnels.
As preferred, the funnel backup pad includes first splint and the second splint of arranging from top to bottom, and first splint and second splint are parallel to each other and relative position all seted up the matching and inlay and establish the funnel of the diameter size of funnel department inlays establishes the hole, first splint with the corresponding position department of second splint all sets up the pillar and inlays establishes the hole to fixed connection at least three pillar.
Compared with the prior art, the utility model has the following beneficial effects:
by combining the separation principle of the Bellman funnel method, the device can reasonably utilize the space, is simple to operate, and can simultaneously separate a plurality of samples. When collecting the root knot nematode, need remove the supporter in the part of collecting the second-instar larva of root knot nematode can, rely on funnel and culture dish to collect, when the second-instar larva is collected to needs, then place the supporter, the funnel does not need work this moment, and the device can collect the root knot nematode, can collect the second-instar larva again, can separate the root knot nematode in the soil according to the experiment demand of difference, can collect the second-instar larva of root knot nematode again.
Drawings
FIG. 1 is a schematic structural view of an embodiment of the device for collecting second instar larvae of Meloidogyne incognita according to the present invention.
Fig. 2 is a schematic view showing the structure of the apparatus of the present invention comprising a means for collecting second instar larvae of root knot nematodes.
In FIGS. 1-2: 1 mesh screen, 2 first faces, 3 steel rings, 4 bracing pieces, 5 culture dishes, 6 first spaces, 7 bases, 8 grooves, 9 pillars, 10 funnels, 11 switches, 12 first clamping plates, 13 second clamping plates and 14 pillar embedding sleeves.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
As shown in FIGS. 1 to 2, FIG. 1 is a schematic structural view of an embodiment of the device for collecting second instar larvae of Meloidogyne incognita according to the present invention.
Fig. 2 is a schematic view showing the structure of the apparatus of the present invention comprising a means for collecting second instar larvae of root knot nematodes.
With reference to fig. 1-2, the present invention provides a device for collecting second instar larvae of root-knot nematodes, comprising:
a mesh screen 1 having a first face 2 for placing root knot nematode second instar larva egg masses and a second face opposite to the first face 2;
a support body, which is provided with a through hole penetrating the upper and lower surfaces of the support body and matching the shape of the mesh screen 1, wherein the side surface of the mesh screen 1 is abutted against the inner side surface of the corresponding through hole so that the mesh screen 1 can be detachably and fixedly arranged in the through hole;
a culture dish 5 which is provided with an opening and a culture chamber, wherein the support body is placed on the bottom surface of the culture dish 5;
a first space 6 is formed by a space surrounded by a second surface of the mesh 1, a part of the bottom surface of the culture dish 5 opposite to the second surface, and a part of the inner side surface of the support body sandwiched between the two surfaces, and the first space 6 communicates with the culture chamber.
For the first space 6, a boundary is defined by a second face, which in this application is defined as the second face of the mesh, which belongs to the mesh, a part of the bottom face of the culture dish 5 opposite to the second face, and a part of the inner side surface of the support body sandwiched between the two faces, but for the definition of the second face here of the first space 6, what is actually to be expressed is a closed (i.e. no through holes are provided through the upper and lower sides of the face) face, which is flush with the second face of the mesh 1.
When the part is used, the hatching process of the second instar larvae is as follows: placing the mesh screen 1 in a support body, placing the support body with the mesh screen 1 in a culture chamber of a culture dish 5, injecting a proper amount of clear water until the mesh screen 1 just submerges, selecting a proper amount of root-knot nematode egg masses in the mesh screen 1, waiting for incubation of second-instar larvae of the root-knot nematodes for 2-7 days, and then collecting the second-instar larvae of the root-knot nematodes for experimental needs. The second-instar larva of hatching can move about to first space 6 through mesh screen 1, in this application, first space 6 and cultivation cavity intercommunication, it is interior except that the outer cavity of this support is linked together to indicate first space 6 and cultivation cavity in essence, and then, the second-instar larva can move about to the cultivation cavity in great space by this first space 6, do not restrict and move about in first space 6, the scope of moving about is big, dissolved oxygen is good, the nematode activity is good, the nematode is very suitable for the reserve experiment, and set up through this intercommunication, make and cultivate the indoor moving about that all has second-instar larva of cavity, when collecting, can directly follow the cultivation cavity internal uptake and take out, conveniently collect.
In one embodiment, the support body comprises:
the steel ring 3 is provided with a circular through hole;
the upper ends of the support rods 4 are fixed on the lower surface of the steel ring 3 at intervals, and the lower ends of the support rods 4 are placed on the bottom surface of the culture dish 5;
the first space 6 communicates with the culture chamber through the gap between the support rods 4.
In some embodiments, the number of the support rods 4 is three, and the support rods are arranged in an equilateral triangle, so that the steel ring 3 can be stably supported.
In some embodiments, the support rods 4 are 2mm in diameter and 2mm in height; the steel ring 3 is formed by embedding an inner steel ring and an outer steel ring, the height of the steel ring 3 is 8mm, and the wall thickness is 2 mm.
In other embodiments the mesh screen 1 has a diameter of 50mm and a mesh size of 50-500 mesh, preferably the mesh size of the mesh screen 1 is 200 mesh, the mesh size being arranged to facilitate passage of second instar larvae through the mesh screen 1 into the first space 6.
In some embodiments, the mesh screen 1 is disposed at a middle position of the through hole in an up-down direction, and the first surface 2 of the mesh screen 1 and a part of the inner side surface of the steel ring 3, which is upward from the first surface 2, enclose an egg mass placing space for placing egg masses. Through setting up mesh screen 1 in intermediate position department to through the setting of placing the space, can make the impurity in the egg piece that is submerged by water be located this placing the space as far as possible, make the nematode of later stage collection comparatively clean.
As shown in fig. 2, the present invention also provides an apparatus comprising a member for collecting second-instar larvae of root-knot nematodes, which is the above-mentioned member for collecting second-instar larvae of root-knot nematodes, the apparatus further comprising:
the base 7 is provided with a plurality of grooves 8 for accommodating the corresponding components for collecting the second-instar larvae of the root-knot nematodes;
at least three struts 9, wherein the at least three struts 9 are fixedly arranged on the periphery of the base 7 and extend upwards;
the at least three upwards extending support columns 9 are fixedly connected with the funnel support plate, the funnel support plate is arranged parallel to the base 7 and is provided with a plurality of funnel embedding holes penetrating through the upper surface and the lower surface of the funnel support plate, and the funnel embedding holes are in one-to-one correspondence with the grooves 8 on the base;
a plurality of funnels 10, the path end of funnel 10 passes funnel inlays establishes the hole, and the orientation is corresponding the direction setting of groove 8, just the switch 11 of funnel 10 is installed to the path end.
The device is adopted to separate the soil root-knot nematodes: the separation of the soil root-knot nematode only needs to take out the mesh screen 1 which cannot be used, the Bellman funnel method separation principle is adopted, wet tissues are taken and washed by tap water, the wet tissues are naturally dried or dried, 10g of soil with the root-knot nematode is weighed, the soil is tied by a rubber band to be in a globular shape and is placed in the funnel 10, the funnel switch 11 is closed, clear water is slowly injected into the funnel 10 until the wet tissues are about 10mm, after the funnel is kept still for 24-72h, the funnel switch 11 is slowly opened, the culture dish 5 in the base 7 collects the separated root-knot nematode, and the root-knot nematode can be checked and counted under a microscope or used for other experimental purposes.
The device can reasonably utilize space, is simple to operate, and can separate a plurality of samples at the same time. When collecting the root knot nematode, need remove the supporter in the part of collecting the second-instar larva of root knot nematode can, rely on funnel 10 and culture dish 5 to collect, when the second-instar larva is collected to needs, then place the supporter (have the same technological effect), funnel 10 need not work this moment, and the device can collect the root knot nematode, can collect the second-instar larva again, can be according to the experimental demand of difference, can separate the root knot nematode in the soil, can collect the second-instar larva of root knot nematode again.
In some embodiments, the number of the pillars 9 is four, as shown in fig. 2, and the pillar fitting sleeve 14 is provided at the middle portion, the funnel support plate includes a first clamping plate 12 and a second clamping plate 13 which are arranged up and down, the positions of the first clamping plate 12 and the second clamping plate 13 which are parallel to each other and opposite to each other are provided with funnel fitting holes matching the diameter of the fitted funnel 10, and the corresponding positions of the first clamping plate 12 and the second clamping plate 13 are provided with pillar fitting holes to fixedly connect the four pillars 9.
Certainly, in order to improve compactness, a sealing gasket can be arranged at the movable contact part of the funnel 10 and the first clamping plate 12 and the second clamping plate 13; the first clamping plate 12 and the second clamping plate 13 are both connected with a pillar 9 for stabilization, and certainly, in order to improve compactness, a sealing ring can be arranged at the movable contact part of the first clamping plate and the second clamping plate; the double-clamping plate structure can improve the stability of the separating device to the maximum extent.
In some embodiments, the first clamping plate 12 is uniformly provided with 8 circular holes with the diameter of 98 mm; 8 circular holes with the diameter of 45mm are uniformly formed in the second clamping plate 13, and 8 funnels 10 are correspondingly placed; the diameter of the opening of the funnel 10 is 100 mm; the culture dish 5 has a diameter of 90 mm.
In some embodiments, the base 7 is provided with a plurality of grooves 8 matching the number of the parts for collecting the second-instar larvae of the root-knot nematodes for accommodating the corresponding parts for collecting the second-instar larvae of the root-knot nematodes, and the base 7 is provided with 8 grooves 8 with the diameter of 92mm and the depth of 2-3mm corresponding to the center of the funnel 10. As shown in FIG. 2, one culture dish 5 is placed in each well 8.
In some embodiments, the first clamping plate 12, the second clamping plate 13, the base 7, the funnel 10 and the culture dish 5 are made of plastic; the mesh screen 1, the steel ring 3 and the support rod 4 are all made of 304 stainless steel materials.
Claims (10)
1. A kit for collecting second instar larvae of root knot nematodes, said kit comprising:
a mesh screen having a first side for housing root knot nematode second instar larva egg masses and a second side opposite the first side;
the supporting body is provided with a through hole which penetrates through the upper surface and the lower surface of the supporting body and is matched with the shape of the mesh screen, and the side surface of the mesh screen is abutted against the inner side surface of the corresponding through hole so that the mesh screen can be detachably and fixedly arranged in the through hole;
the culture dish is arranged in an opening manner and is provided with a culture chamber, and the support body is placed on the bottom surface of the culture dish;
a first space is formed by a space surrounded by the second surface of the mesh screen, a part of the bottom surface of the culture dish opposite to the second surface, and a part of the inner side surface of the support body sandwiched between the two surfaces, and the first space is communicated with the culture chamber.
2. The member for collecting second instar larvae of root knot nematodes according to claim 1, wherein said support comprises:
a steel ring having a circular through hole;
the upper ends of the support rods are fixed on the lower surface of the steel ring at intervals, and the lower ends of the support rods are placed on the bottom surface of the culture dish;
the first space is communicated with the culture chamber through gaps among the support rods.
3. The device for collecting second instar larvae of meloidogyne according to claim 2, wherein the mesh screen is disposed at a middle position of the through hole in an up-down direction, and a first surface of the mesh screen and a part of an inner side surface of the steel ring, which is upward from the first surface, enclose an egg mass placing space for placing the egg mass.
4. The device for collecting second instar larvae of meloidogyne according to claim 2, wherein the number of the support rods is three, and the support rods are arranged in an equilateral triangle.
5. The member for collecting second instar larvae of root knot nematodes according to claim 4, wherein said supporting rods have a diameter of 2mm and a height of 2 mm.
6. The device for collecting second instar larvae of meloidogyne according to claim 2, wherein the steel ring is formed by an inner steel ring and an outer steel ring which are embedded, and the steel ring has a height of 8mm and a wall thickness of 2 mm.
7. The device for collecting second instar larvae of meloidogyne according to claim 1, wherein the mesh screen has a diameter of 50mm and a mesh number of 50 to 500 meshes.
8. The device for collecting second instar larvae of meloidogyne according to claim 7, wherein the mesh number of the mesh screen is 200 meshes.
9. An apparatus comprising a member for collecting second-instar larvae of root-knot nematode according to any one of claims 1 to 8, wherein the member for collecting second-instar larvae of root-knot nematode further comprises:
the base is provided with a plurality of grooves for accommodating the corresponding parts for collecting the second-instar larvae of the root-knot nematodes;
the at least three struts are fixedly arranged on the periphery of the base and extend upwards;
the funnel supporting plate is arranged in parallel to the base and is provided with a plurality of funnel embedding holes penetrating through the upper surface and the lower surface of the funnel supporting plate, and the funnel embedding holes are in one-to-one correspondence to the grooves in the base;
the small-diameter ends of the funnels penetrate through the funnel embedding holes and face the corresponding grooves in the direction, and the small-diameter ends are provided with switches of the funnels.
10. The device of claim 9, wherein the funnel support plate comprises a first clamping plate and a second clamping plate which are arranged up and down, the first clamping plate and the second clamping plate are parallel to each other and are provided with funnel embedding holes corresponding to the diameter of the embedded funnel, and the first clamping plate and the second clamping plate are provided with pillar embedding holes corresponding to the at least three pillars.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121966320.4U CN216088376U (en) | 2021-08-20 | 2021-08-20 | Part for collecting root-knot nematode second-instar larvae and device comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121966320.4U CN216088376U (en) | 2021-08-20 | 2021-08-20 | Part for collecting root-knot nematode second-instar larvae and device comprising same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216088376U true CN216088376U (en) | 2022-03-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121966320.4U Active CN216088376U (en) | 2021-08-20 | 2021-08-20 | Part for collecting root-knot nematode second-instar larvae and device comprising same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216088376U (en) |
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2021
- 2021-08-20 CN CN202121966320.4U patent/CN216088376U/en active Active
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