CN220734078U

CN220734078U - Combined plant nematode egg hatching device

Info

Publication number: CN220734078U
Application number: CN202321810456.5U
Authority: CN
Inventors: 陈立杰; 段辰君; 王媛媛; 朱晓峰; 范海燕; 刘晓宇; 段玉玺
Original assignee: Shenyang Agricultural University
Current assignee: Shenyang Agricultural University
Priority date: 2023-07-11
Filing date: 2023-07-11
Publication date: 2024-04-09
Anticipated expiration: 2033-07-11

Abstract

The utility model discloses a combined plant nematode egg hatching device, which relates to the technical field of plant nematode egg hatching, and comprises a large culture dish body, wherein an annular edge is fixed at the top of the large culture dish body, two side openings are formed in one side of the annular edge, a constraint ring is arranged in the large culture dish body, a small culture dish is arranged between the inner walls of the constraint ring, two nylon adjusting belts are fixed on the outer surface of the constraint ring, and the two nylon adjusting belts correspondingly penetrate through the interiors of the two side openings.

Description

Combined plant nematode egg hatching device

Technical Field

The utility model relates to the technical field of plant nematode egg hatching, in particular to a combined plant nematode egg hatching device.

Background

The root-knot nematode is a plant root system obligate parasitic nematode, the root-knot nematode mainly damages the roots of various vegetables, the lateral roots and fibrous roots are more normally increased, and the fibrous roots of the young roots are formed into milky white tumor-shaped root knots with different spherical or conical sizes, and the roots are in a candid shape, so that the overground parts of the damaged plants grow short and slow, the leaf color is abnormal, the result is less, the yield is low, even the plants die in advance, and the problem can be effectively solved by cultivating resistant varieties.

In the process of cultivating resistant varieties, screening and obtaining of resistant germplasm are critical, a large number of root knot nematode larvae are needed to be used for inoculation experiments in the process of screening the resistant germplasm, and a hatching device convenient to cultivate is needed to hatch plant line eggs.

Disclosure of Invention

(one) solving the technical problems

The utility model provides a combined plant nematode egg hatching device, which is characterized in that a small culture dish is arranged in a large culture dish, so that the small culture dish can float on culture solution, and the outer surface of the small culture dish is coated with filter cloth, so that the culture solution can conveniently soak plant nematode eggs, the hatching efficiency is improved, and people can conveniently take out the plant nematode eggs.

(II) technical scheme

In order to solve the technical problems, the utility model provides the following technical scheme:

the utility model provides a modular plant nematode ovum hatching apparatus, includes big culture dish body, the top of big culture dish body is fixed with the annular limit, two side mouths have been seted up to one side on annular limit, the inside of big culture dish body is provided with the restriction ring, be provided with little culture dish between the inner wall of restriction ring, the surface mounting of restriction ring has two nylon adjustment area, two nylon adjustment area all corresponds the inside that runs through in two side mouths.

Preferably, the outer surface of the annular edge is provided with two positioning holes, and the outer surfaces of the two nylon adjusting belts are provided with a plurality of clamping holes at equal intervals.

Preferably, positioning nails are arranged in the two positioning holes, and an annular groove is formed in the inner wall of the constraint ring.

Preferably, a plurality of arc-shaped spring pieces are fixed on the inner wall of one side of the annular groove along the circumferential direction at equal intervals, and one ends of the arc-shaped spring pieces extend outwards.

Preferably, the bottom of little culture dish is the opening form, the surface cladding of little culture dish has the filter cloth, the filter cloth seals the bottom of little culture dish, and the edge of filter cloth extends to the top of little culture dish.

Preferably, the outer surface of the small culture dish is provided with a locking ring, a rubber ring is fixed between the inner walls of the locking ring, and the inner walls of the rubber ring press the filter cloth on the outer surface of the small culture dish.

(III) beneficial effects

Compared with the prior art, the utility model has the following beneficial effects:

1. when the device is used, firstly, the bottom of a small culture dish is arranged to be open, then the bottom is covered by filter cloth, the small culture dish is tightly pressed by a rubber ring in a locking ring, redundant cloth is cut off, then the small culture dish is placed in a large culture dish body, a small amount of sterile water is poured into the small culture dish, the picked oocysts are placed in the small culture dish, the oocysts are lightly washed for 2-3 times by using a 0.5% sodium hypochlorite solution, the residual sodium hypochlorite is washed by using the sterile water for 2-3 times, sterile water is poured into the large culture dish body, the water amount is higher than the oocysts, a culture dish cover is covered, tinfoil covers the large culture dish body, the large culture dish body is hatched at the room temperature of 25 ℃ in a dark place, and the collection time can be prolonged to 10-14 days for obtaining more nematodes.

2. According to the utility model, in actual use, the clamping holes on the nylon adjusting belt are matched with the positioning nails, so that the position of the constraint ring can be conveniently changed, meanwhile, the arc-shaped spring piece is arranged in the annular groove at the inner side of the constraint ring, so that the small culture dish is conveniently constrained and fixed, and meanwhile, when the filter cloth is constrained, the filter cloth is conveniently compacted through the rubber ring at the inner side of the locking ring.

Drawings

Fig. 1 is a schematic perspective view of a combined plant nematode egg hatching apparatus according to the present utility model;

FIG. 2 is a schematic cross-sectional perspective view of a confinement ring in a combined plant nematode egg hatching apparatus according to the present utility model;

fig. 3 is a schematic cross-sectional perspective view of a small culture dish in a combined plant nematode egg hatching device according to the present utility model.

Reference numerals: 1. a large culture dish body; 2. an annular edge; 3. a side port; 4. positioning holes; 5. positioning nails; 6. a confinement ring; 7. nylon adjusting belts; 8. a clamping hole; 9. a small petri dish; 10. a locking ring; 11. an annular groove; 12. an arc-shaped spring piece; 13. a filter cloth; 14. a rubber ring.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

Embodiment 1, as shown in fig. 1-3, the utility model provides a technical scheme of a combined plant wire worm egg hatching device: including big culture dish body 1, the top of big culture dish body 1 is fixed with annular limit 2, and two side mouths 3 have been seted up to one side of annular limit 2, and the inside of big culture dish body 1 is provided with restriction ring 6, is provided with little culture dish 9 between the inner wall of restriction ring 6, and the external surface of restriction ring 6 is fixed with two nylon adjustment area 7, and two nylon adjustment area 7 all correspond to run through in the inside of two side mouths 3.

The whole embodiment 1 achieves the effects that when the device is used, firstly, the bottom of a small culture dish 9 is arranged to be open, then, after the bottom is wrapped by a filter cloth 13, the bottom is tightly pressed by a rubber ring 14 in a locking ring 10, redundant cloth is sheared off, then, the small culture dish 9 is placed in an enlarged culture dish body 1, a small amount of sterile water is poured into the small culture dish 9, the picked oocysts are placed in the small culture dish 9, the oocysts are lightly washed for 2-3 times by using a 0.5% sodium hypochlorite solution, the sterile water is used for washing the residual sodium hypochlorite for 2-3 times, sterile water is poured into the large culture dish body 1, the water quantity is higher than the oocysts, a culture dish cover is covered, the large culture dish body 1 is wrapped by tinfoil, the water is hatched at the room temperature of about 25 ℃, and the collection time is collected for 3-5 days, and the collection time can be prolonged to 10-14 days for obtaining more nematodes.

Embodiment 2, as shown in fig. 1-3, two locating holes 4 have been seted up to the surface of annular limit 2, a plurality of draw-in holes 8 have been seted up to the surface equal interval of two nylon adjustment belts 7, the inside of two locating holes 4 all is provided with locating nail 5, annular groove 11 has been seted up to the inner wall of holding ring 6, one side inner wall of annular groove 11 is fixed with a plurality of arc spring pieces 12 along the circumferencial direction equidistance, the one end of a plurality of arc spring pieces 12 all extends to the outside, the bottom of little culture dish 9 is the opening form, the surface cladding of little culture dish 9 has filter cloth 13, filter cloth 13 seals the bottom of little culture dish 9, and the edge of filter cloth 13 extends to the top of little culture dish 9, the surface of little culture dish 9 is provided with locking ring 10, be fixed with rubber ring 14 between the inner wall of locking ring 10, the inner wall of rubber ring 14 pressfitting filter cloth 13 on the surface of little culture dish 9.

The effect that its whole embodiment 2 reached is, when in actual use, through the card hole 8 cooperation locating nail 5 on the nylon adjustment band 7 for can be convenient for change the position of restriction ring 6, set up arc spring piece 12 in the ring channel 11 inside the restriction ring 6 simultaneously, be convenient for retrain fixedly to little culture dish 9, when retraining filter cloth 13 simultaneously, through the rubber ring 14 of locking ring 10 inboard, make convenient to compress tightly filter cloth 13.

Working principle: when the device is used, firstly, the bottom of the small culture dish 9 is set to be open, then after the bottom is wrapped by the filter cloth 13, the rubber ring 14 inside the locking ring 10 is used for compacting, redundant cloth is cut off, then the small culture dish 9 is placed in the large culture dish body 1, a small amount of sterile water is poured into the small culture dish 9, the picked oocysts are placed in the small culture dish 9, the oocysts are lightly washed 2-3 times by using 0.5% sodium hypochlorite solution, then 2-3 times are washed by using the sterile water, residual sodium hypochlorite is washed, sterile water is poured into the large culture dish body 1, the water amount is higher than the oocysts, the culture dish cover is covered, the large culture dish body 1 is wrapped by tinfoil, the hatching is carried out at the room temperature of about 25 ℃ for 3-5 days in a dark way, in order to obtain more nematodes, the collection time can be prolonged to 10-14 days, in the actual use, the position of the constraint ring 6 can be conveniently changed by matching the locating pin 5 through the clamping hole 8 on the nylon adjusting belt 7, meanwhile, the filter cloth is conveniently compacted by the annular groove 13, and the filter cloth is conveniently compacted by the filter cloth 13 when the filter cloth is fixed inside the constraint ring 11.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a combination formula plant nematode ovum hatching apparatus, includes big culture dish body (1), its characterized in that: the top of big culture dish body (1) is fixed with annular limit (2), two side mouths (3) have been seted up to one side of annular limit (2), the inside of big culture dish body (1) is provided with restriction ring (6), be provided with little culture dish (9) between the inner wall of restriction ring (6), the surface mounting of restriction ring (6) has two nylon adjustment area (7), two nylon adjustment area (7) all correspond the inside that runs through in two side mouths (3).

2. A modular plant nematode egg hatching apparatus according to claim 1, wherein: two locating holes (4) are formed in the outer surface of the annular edge (2), and a plurality of clamping holes (8) are formed in the outer surface of the nylon adjusting belt (7) at equal intervals.

3. A modular plant nematode egg hatching apparatus according to claim 2, wherein: the inside of two locating holes (4) is provided with locating nails (5), and the inner wall of the constraint ring (6) is provided with an annular groove (11).

4. A modular plant nematode egg hatching apparatus according to claim 3, wherein: a plurality of arc-shaped spring pieces (12) are fixed on the inner wall of one side of the annular groove (11) at equal intervals along the circumferential direction, and one ends of the arc-shaped spring pieces (12) extend outwards.

5. A modular plant nematode egg hatching apparatus according to claim 4, wherein: the bottom of little culture dish (9) is the opening form, the surface cladding of little culture dish (9) has filter cloth (13), the bottom of filter cloth (13) with little culture dish (9) is sealed, and the edge of filter cloth (13) extends to the top of little culture dish (9).

6. A combination plant nematode egg hatching apparatus according to claim 5, wherein: the outer surface of the small culture dish (9) is provided with a locking ring (10), a rubber ring (14) is fixed between the inner walls of the locking ring (10), and the inner walls of the rubber ring (14) press the filter cloth (13) on the outer surface of the small culture dish (9).