CN219260048U - Full embryo rotation culture instrument - Google Patents
Full embryo rotation culture instrument Download PDFInfo
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- CN219260048U CN219260048U CN202223401179.0U CN202223401179U CN219260048U CN 219260048 U CN219260048 U CN 219260048U CN 202223401179 U CN202223401179 U CN 202223401179U CN 219260048 U CN219260048 U CN 219260048U
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Abstract
The application relates to the field of embryo tissue culture containers and discloses a full embryo rotation culture instrument. In this application, rotatory culture appearance includes three kind gas mixing control system, adjustable speed rotatory thing dish that carries, pressure control system, gas concentration detecting system, PH detection device, culture medium factor detection device, temperature control system, gas wetting bottle, oxygen concentration excessive alarm device, box that carries, three kind gas mixing control system, adjustable speed thing rotary disk, pressure control system, gas filtration system's system etc.. The incubator establishes a system capable of simulating intrauterine culture in vitro, builds a small environment for embryos, can regulate the temperature, and breaks the bottleneck of embryo development process research which is covered by the uterus. A model was constructed that could be used to study the effects of different perturbations on embryo development processes, and also to demonstrate that mammalian embryo development and organogenesis processes can be completely and adequately reproduced in vitro culture vessels.
Description
Technical Field
The application belongs to the technical field of embryo tissue culture containers, and particularly relates to a full embryo rotation culture instrument.
Background
After the ovum and sperm of mammal form zygote in the ampulla of oviduct, the development of individual is started, after the fertilization of sperm and ovum in the ampulla of oviduct is completed, embryo starts to move to uterine horn direction, embryo is split in the course of moving. Animals vary in species, and the time and stage of embryo migration into the uterus vary. The fertilized ovum of the mouse moves 72 mice in the oviduct and enters the uterus when the fertilized ovum develops to the morula stage; after the fertilized eggs of the cattle migrate in the oviduct for 72-84 hours, entering the uterus at 8-16 cell stage; pig fertilized eggs enter uterus at 4-6 cell stage in 46-48 mice with oviduct migration. Development of embryos before entering the uterine horn is primarily dependent on the manipulation of the materials stored in the ovum and the oviduct environment. Therefore, the temperature, the rotating speed and the pressure can be adjusted, and the method can be applied to embryo culture of different types.
Fisher and Bavister (1993) measured the concentration of oxygen in hamster, rabbit and macaque oviduct and uterus with sensitive clicks, found that the oxygen concentration in mid-term was only 40% of air. The parameters and the oxygen concentration of the rabbit in the embryo transfer period are lower, so the low-oxygen environment is very beneficial to controlling the peroxide concentration in the embryo living environment and promoting the embryo development.
Nutrient substances: in early embryo development, different nutrients, energy substances and electrolytes are different; the concentration of some amino acids in the fallopian tube and uterine fluid is significantly higher than in the blood, such as glycine, taurine and pyruvic acid. The oviduct epithelium can secrete various protein factors after ovulation, and the research finds that the protein factors regulate early development of the embryo through the way of combining with receptors on the surface of the embryo or being directly pinocytosis by the embryo, and the like.
Energy substance: the energy substances supporting embryo development in the fallopian tube and uterine cavity are mainly pyruvic acid, lactic acid and glucose, and their concentrations are constantly changing.
An electrolyte: in embryo development period, the concentration of K+ and HCO 3-in oviduct is higher, the concentration of K+ is 3 times that of blood, the concentration of HCO3 is increased from 35mmol/L before ovulation to 90mmol/L after ovulation, the PH change in oviduct mainly depends on the change of HCO 3-because the required pH value of the ampulla at fertilization is maintained at 7.6-7.8, and the ideal pH value at embryo development is 7.1-7.3, so that the PH can be regulated to have a critical effect on embryo development.
In the process of in vitro culturing of mammalian embryo, a whole embryo rotary culture instrument is needed, but the existing embryo tissue has the following disadvantages when culturing: often cultivate in the culture dish, but current culture dish can't rotate, and the experimenter can't directly operate the culture dish of other positions department, also touch the container very easily during the operation simultaneously, and it is very inconvenient to use.
2. The growth condition cannot be observed, namely, the heat preservation box body is provided with an observation window, and the culture vessel is made of transparent glass;
3. mammalian embryos are cultured using the original method and apparatus to easily adhere to the bottom of the container-the present application provides apparatus that uses low speed rotation so that embryos do not adhere to the bottom of the culture container;
4. the original method and equipment cannot be used for integral heating, namely the temperature-adjustable rotary carrying tray is provided with the temperature-adjustable heat-insulation box body, so that the whole speed-adjustable rotary carrying tray can be heated;
5. the original method and equipment cannot be used for regulating and controlling the gas concentration, namely the device comprises a concentration regulating device for three gases, three different gases can be mixed according to the required proportion, and the concentration of the gases entering the rotary ventilation carrying disc can be detected;
6. the original method and equipment can not be used for adjusting the PH value, namely the equipment is provided with a PH detection device, and the PH value of the culture medium can be controlled by adjusting the concentration of carbon dioxide;
7. the pressure cannot be regulated using the original method and apparatus-the apparatus of the present application has a pressure regulating device that can provide pressures up to 30 psi;
8. the original method and equipment are used without a detection device, namely the equipment comprises a culture medium factor detection device which can detect indexes such as ph value, glycine, taurine, pyruvic acid, lactic acid, glucose and the like;
9. the original method and equipment can not control the gas concentration in the container, 2 hole sites are arranged outside the object carrying rotary disc of the equipment, 24 glass vial containers can be hung outside the hole sites, a special air passage is arranged in the speed-adjustable rotary ventilation object carrying disc, and three gas mixing control systems can be uniformly distributed in each vial.
Disclosure of Invention
The purpose of the present application is: in order to solve the problems set forth above, a whole embryo rotation incubator is provided.
The technical scheme adopted by the application is as follows: the whole embryo rotary culture instrument comprises three gas mixing control systems, a speed-adjustable rotary ventilation carrying disc, a pressure control system, a gas concentration detection system, a PH detection device, a culture medium factor detection device, a temperature control system, a gas wetting bottle, an oxygen concentration excess alarm device and a box body, wherein the three gas mixing control systems, the speed-adjustable carrying rotary disc, the pressure control system, the gas concentration detection system, the gas filtration system, the PH detection device, the culture medium factor detection device, the temperature control system, the gas wetting bottle and the oxygen concentration excess alarm device are positioned in the box body;
the surface of the speed-adjustable rotary ventilation carrying disc is provided with an air inlet flow passage, the side edge of the air inlet flow passage is provided with an air outlet flow passage, the surface of the speed-adjustable rotary ventilation carrying disc is provided with a plurality of sensor mounting points, and a sealing ring is adhered to the surface of the speed-adjustable rotary ventilation carrying disc close to the edge.
In a preferred embodiment, the whole embryo rotary incubator is provided with a box, a control panel is arranged above the box, and a gas humidifying bottle is arranged above the box.
In a preferred embodiment, the bottom of full embryo rotation culture appearance is provided with mounting plate, mounting plate's upper surface fixed mounting has the mounting bracket, and the surface of mounting bracket is provided with driving motor, driving motor's top is provided with gas electric rotary joint, the one end that gas electric rotary joint kept away from the mounting bracket is provided with the air inlet, the bottom of air inlet is provided with oxygen sensor joint, mounting plate's upper surface is located driving motor's side and installs the sensor, the both sides of mounting bracket are provided with the blake bottle, one side end that blake bottle and mounting bracket contacted is provided with the rubber circle, the rubber circle is provided with business turn over air current way with the blake bottle other end, be provided with the relief valve on one side mounting bracket that mounting plate's upper surface and gas electric rotary joint are relative.
In a preferred embodiment, the carrying tray comprises a turntable carved with a special air passage, an anti-leakage sealing ring, 24 screw ports capable of externally hanging glass vials, a breathable stand column containing gears, an adjustable speed stepping motor and a conveyor belt.
In a preferred embodiment, the turntable is provided with an anti-oxidation coating both inside and outside.
In a preferred embodiment, a quick-release nozzle is disposed outside the turntable.
In summary, due to the adoption of the technical scheme, the beneficial effects of the application are as follows:
in this application, the culture apparatus of this application designs uniquely, compact structure, independent power supply, convenient operation. The incubator establishes a system capable of simulating intrauterine culture in vitro, builds a small environment for embryos, can regulate the temperature, and breaks the bottleneck of embryo development process research which is covered by the uterus. A model was constructed that could be used to study the effects of different perturbations on embryo development processes, and also to demonstrate that mammalian embryo development and organogenesis processes can be completely and adequately reproduced in vitro culture vessels.
Drawings
FIG. 1 is a schematic diagram of a culture solution instrument according to the present application;
FIG. 2 is a schematic view of the structure of the carrying tray in the present application;
FIG. 3 is a cross-sectional view of the tray of the present application;
fig. 4 is a schematic view of the surface structure of the carrying disc in the present application.
The marks in the figure: 1-driving motor, 2-oxygen sensor joint, 3-air inlet, 4-gas electricity rotary joint, 5-sensor, 6-relief valve, 7-blake bottle, 8-rubber circle, 9-business turn over air flow channel, 10-control panel, 11-gas humidification bottle, 12-inlet channel, 13-outlet channel, 14-sensor mounting point, 15-sealing washer.
Detailed Description
For the purposes of making the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, are intended to be within the scope of the present application.
With reference to figures 1-4 of the drawings,
examples:
the application discloses a full embryo rotation culture instrument. The rotary culture instrument comprises three gas mixing control systems, a speed-adjustable rotary ventilation carrying disc, a pressure control system, a gas concentration detection system, a PH detection device, a culture medium factor detection device, a temperature control system, a gas wetting bottle, an oxygen concentration excess alarm device and a box body, wherein the three gas mixing control systems, the speed-adjustable carrying rotary disc, the pressure control system, the gas concentration detection system, the gas filtration system, the PH detection device, the culture medium factor detection device, the temperature control system, the gas wetting bottle and the oxygen concentration excess alarm device are positioned in the box body;
the main functions of the rotary culture instrument are as follows: providing a microenvironment for each embryo, simulating an in vivo growth ring 1: 3 gas exchange: o (O) 2 :0-100% (adjustable); CO 2 :0-100% (adjustable)
N 2 : is not adjustable;
2. control pressure: 0-20psi
3. And (3) controlling the temperature: constant temperature of 37 DEG C
4. And (3) rotation: 5-500rpm, providing gas exchange
5. A container: the culture vials were custom-made by labeling 4-7ml.
The core technology of the application and the special flow channel design can ensure that the gas in each externally hung small bottle is uniformly distributed;
the surface of the speed-adjustable rotary ventilation carrying disc is provided with an air inlet flow channel 12, the side edge of the air inlet flow channel 12 is provided with an air outlet flow channel 13, the surface of the speed-adjustable rotary ventilation carrying disc is provided with a plurality of sensor mounting points 14, and a sealing ring 15 is adhered to the surface of the speed-adjustable rotary ventilation carrying disc near the edge.
The rotary disk is provided with a sensor connected to the inside of the rotary disk, and the gas parameters are monitored at the position of the gas discharge channel, so that each preset index in the culture bottle can be monitored in real time.
Firstly, externally hanging 24 glass vial containers on hole sites of an adjustable speed rotary ventilation carrying disc by using sealing rubber plugs; the gas cylinders of three different gases are externally connected with the three gas mixing control systems, the gas mixing control systems are opened, the concentrations of the three gases are regulated, the proportion is 100%, and after the three different gases are uniformly mixed through the gas mixing control systems, heat source substances such as bacteria are filtered through the gas filtering system, so that the gas entering the small bottle is sterile.
Then the gas concentration detection system is used for obtaining whether the gas concentration is accurate or not; the purified water is filled in the gas wetting bottle, so that the humidity of the gas entering the turntable is ensured to be more than 93%; then the mixture is introduced into the carrying rotary disk through a pipeline, and then the mixture is distributed into 24 glass small bottle containers through air passages in the carrying rotary disk.
The glass vial container is internally provided with a PH detection device and a culture medium factor detection device, and can detect the culture medium factors of indexes such as PH value, glycine, taurine, pyruvic acid, lactic acid, glucose and the like in the vial; a control circuit is arranged in the box body, the temperature can be regulated, the direct current motor is connected with the control circuit, and the object carrying rotary disk is connected with the speed reducer through a transmission shaft; the rotating speed regulating valve is connected with the direct current motor; the outer wall of the box body is provided with an observation window and a display screen, the oxygen concentration excessive alarming device is connected with a main power supply, and if the oxygen concentration inside the box body is detected to be excessive, an alarm sound is given out and the power supply is cut off.
The culture instrument has unique design, compact structure, independent power supply and convenient operation. The incubator establishes a system capable of simulating intrauterine culture in vitro, builds a small environment for embryos, can regulate the temperature, and breaks the bottleneck of embryo development process research which is covered by the uterus. A model was constructed that could be used to study the effects of different perturbations on embryo development processes, and also to demonstrate that mammalian embryo development and organogenesis processes can be completely and adequately reproduced in vitro culture vessels.
The above embodiments are only for illustrating the technical solution of the present application, and are not limiting thereof; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (7)
1. A full embryo rotation culture instrument, characterized in that: the full embryo rotation incubator comprises three gas mixing control systems, an adjustable speed rotation ventilation carrying disc, a pressure control system, a gas concentration detection system, a PH detection device, a culture medium factor detection device, a temperature control system, a gas wetting bottle, an oxygen concentration excess alarm device and a box body, wherein the three gas mixing control systems, the adjustable speed carrying rotating disc, the pressure control system, the gas concentration detection system, the gas filtration system, the PH detection device, the culture medium factor detection device, the temperature control system, the gas wetting bottle and the oxygen concentration excess alarm device are positioned in the box body;
the adjustable speed rotary ventilation carrying disc is characterized in that an air inlet runner (12) is formed in the surface of the adjustable speed rotary ventilation carrying disc, an air outlet runner (13) is formed in the side edge of the air inlet runner (12), a plurality of sensor mounting points (14) are formed in the surface of the adjustable speed rotary ventilation carrying disc, and a sealing ring (15) is bonded to the surface of the adjustable speed rotary ventilation carrying disc close to the edge.
2. A whole embryo rotation incubator as claimed in claim 1, wherein: the whole embryo rotation incubator is characterized in that a box body is arranged outside the whole embryo rotation incubator, a control panel (10) is arranged above the outer part of the box body, and a gas humidifying bottle (11) is arranged above the inner part of the box body.
3. A whole embryo rotation incubator as claimed in claim 1, wherein: the bottom of full embryo rotation culture appearance is provided with mounting plate, mounting plate's upper surface fixed mounting has the mounting bracket, and the surface of mounting bracket is provided with driving motor (1), the top of driving motor (1) is provided with gas electric rotary joint (4), the one end that mounting bracket was kept away from to gas electric rotary joint (4) is provided with air inlet (3), the bottom of air inlet (3) is provided with oxygen sensor joint (2), sensor (5) are installed to the side that mounting plate's upper surface is located driving motor (1), the both sides of mounting bracket are provided with blake bottle (7), one side end that blake bottle (7) contacted with the mounting bracket is provided with rubber circle (8), rubber circle (8) and blake bottle (7) other end are provided with business turn over air flow channel (9), be provided with relief valve (6) on one side mounting bracket that mounting plate's upper surface and gas electric rotary joint (4) are relative.
4. A whole embryo rotation incubator as claimed in claim 1, wherein: the carrying tray comprises a turntable carved with a special air passage, an anti-leakage sealing ring, 24 screw ports capable of being externally hung with glass vials, a breathable stand column containing gears, an adjustable speed stepping motor and a conveyor belt.
5. A whole embryo rotation incubator as claimed in claim 1, wherein: and the inside and the outside of the turntable are both provided with anti-oxidation coatings.
6. A whole embryo rotation incubator as claimed in claim 1, wherein: the rotary table is provided with a quick-dismantling type air receiving nozzle.
7. A whole embryo rotation incubator as claimed in claim 3, in which: the standard of the culture bottle (7) is 4-7ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223401179.0U CN219260048U (en) | 2022-12-19 | 2022-12-19 | Full embryo rotation culture instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223401179.0U CN219260048U (en) | 2022-12-19 | 2022-12-19 | Full embryo rotation culture instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219260048U true CN219260048U (en) | 2023-06-27 |
Family
ID=86865201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223401179.0U Active CN219260048U (en) | 2022-12-19 | 2022-12-19 | Full embryo rotation culture instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219260048U (en) |
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2022
- 2022-12-19 CN CN202223401179.0U patent/CN219260048U/en active Active
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