CN219239661U - Calibrating device of fluorescence quantitative PCR instrument - Google Patents
Calibrating device of fluorescence quantitative PCR instrument Download PDFInfo
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- CN219239661U CN219239661U CN202320166407.6U CN202320166407U CN219239661U CN 219239661 U CN219239661 U CN 219239661U CN 202320166407 U CN202320166407 U CN 202320166407U CN 219239661 U CN219239661 U CN 219239661U
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- 238000003753 real-time PCR Methods 0.000 title claims abstract description 46
- 239000000523 sample Substances 0.000 claims abstract description 35
- 230000003287 optical effect Effects 0.000 claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 7
- 238000005259 measurement Methods 0.000 abstract description 3
- 230000004907 flux Effects 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000010170 biological method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The utility model relates to the technical field of temperature detection of medical instruments, in particular to a calibration device of a fluorescence quantitative PCR instrument, which is characterized in that a host machine provided with a circuit board and three identical calibration modules which are respectively and fixedly arranged on the host machine in a triangle shape are arranged, wherein each calibration module comprises a temperature calibration unit and an optical calibration unit, the center of one calibration module is positioned on a Y-direction central line, and the other two calibration modules are symmetrically arranged at the edge by taking the Y-direction central line as a central line, so that the measurement of the middle and the edge is realized, and the physical detection of the fluorescence quantitative PCR instrument with small flux is realized; the filter is set to be a narrow-band filter, so that the span range is small, and the specific calibration of the wavelength range of a specific reporting fluorescent group can be realized; the temperature data collected by the probe and the optical data collected by the measured fluorescence quantitative PCR instrument are arranged by the calibration module, so that the accuracy and the reliability of the fluorescence quantitative PCR instrument can be effectively reflected.
Description
Technical Field
The utility model relates to the technical field of temperature detection of medical instruments, in particular to a calibrating device of a fluorescent quantitative PCR instrument.
Background
The core technology of the fluorescent quantitative PCR instrument is to add fluorescent groups into a PCR reaction system, monitor the whole PCR reaction process in real time by utilizing fluorescent signal accumulation and finally quantitatively analyze an unknown template through a standard curve, wherein the method mainly relates to temperature and optical two contents, and the accuracy and reliability of temperature and optical physical parameters and determine the accuracy and reliability of the detection result of the fluorescent quantitative PCR instrument.
The current calibration methods for fluorescent quantitative PCR instruments include physical methods and biological methods; the biological method mainly adopts DNA plasmid standard substances to detect sample linearity and sample indication error of the fluorescent quantitative PCR instrument, the method does not directly calibrate optical physical parameters of the fluorescent quantitative PCR instrument, is the judgment of experimental results of combined action of temperature and optics, cannot effectively reflect whether the physical layer of equipment is accurate and reliable, and cannot carry out reverse tracing and process analysis on the accuracy of measurement results; the physical method is provided with a fluorescent quantitative PCR instrument optical simulator which is universally adapted to a 96 Kong Yingguang quantitative PCR instrument and a 48 Kong Yingguang quantitative PCR instrument, and cannot be suitable for physical detection of small-flux fluorescent quantitative PCR instruments such as 16 holes and 32 holes, and besides, the used optical simulator has a universal wavelength range of 400-700nm, has a large wavelength range span, is not calibrated for a single fluorescent probe or fluorescent dye, and does not have the purpose of calibrating the wavelength range of a specific reporting fluorescent group.
Disclosure of Invention
The utility model discloses a calibrating device of a fluorescent quantitative PCR instrument, and aims to solve the technical problems in the prior art.
The utility model adopts the following technical scheme:
the calibrating device of the fluorescent quantitative PCR instrument is used for calibrating the temperature and the optics of the fluorescent quantitative PCR instrument and comprises a host machine provided with a circuit board and three identical calibrating modules which are respectively and fixedly arranged on the host machine in a triangular shape, wherein each calibrating module comprises a temperature calibrating unit and an optics calibrating unit; the top wall of the outer shell body arranged on the host is provided with light holes respectively positioned at the vertexes of the triangle, the center of the light holes positioned in the middle is positioned on the Y-direction central line of the outer shell body, and the bottom of the outer shell body is provided with the circuit board; the temperature calibration unit comprises temperature probes which are respectively fixedly connected to the outer side of the circuit board and positioned at the vertex of the triangle; the optical calibration unit comprises a light source which is arranged on the inner side of the circuit board and projects light to the light transmission hole, and a light filter is arranged between the light source and the top wall.
In some embodiments, the outer casing is a square body, the light-transmitting holes are square holes, the sides of the top wall and the corresponding sides of the square holes are parallel to each other, and the center of the light-transmitting holes is not less than 9cm from the edge of the outer casing.
In some embodiments, the center of the light hole in the middle is on the Y-direction center line, and the other two light holes are symmetrically arranged with the Y-direction center line as a center line.
In some embodiments, the inner diameter of the light hole is equal to the diameter of a reaction hole of a temperature control module of the PCR instrument to be measured.
In some embodiments, the light source comprises an LED light source soldered to the circuit board.
In some embodiments, the filter is placed horizontally on the LED light source, the filter being provided as a narrowband filter, the narrowband filter being suitable for use in the wavelength range of 480-520 nm.
In some embodiments, the temperature probes are downward and perpendicular to the circuit board, the temperature probes comprise a cylinder positioned at the upper part and a cone positioned at the lower end part of the cylinder, and the temperature probes are matched and attached with a measured temperature control module measuring hole of the PCR instrument.
In some embodiments, the temperature probe is fixedly connected with the circuit board through a connecting sheet fixedly arranged on the upper end.
The beneficial effects are that:
the utility model discloses a calibrating device of a fluorescent quantitative PCR instrument, which has the following advantages compared with the prior art:
the calibration device of the fluorescent quantitative PCR instrument comprises a host machine provided with a circuit board and three identical calibration modules which are respectively and fixedly arranged on the host machine in a triangle shape, wherein each calibration module comprises a temperature calibration unit and an optical calibration unit, the center of one calibration module is positioned on a Y-direction central line, the other two calibration modules are symmetrically arranged at the edge by taking the Y-direction central line as a central line, the center distance between the two calibration modules and the edge of the outer shell is not less than 9cm, the measurement of the middle and the edge in the triangle is ensured, the phenomenon of incomplete acquired data caused by the edge effect of the fluorescent quantitative PCR instrument is effectively avoided, and the calibration device is not only suitable for physical detection of the 96 Kong Yingguang quantitative PCR instrument and 48 Kong Yingguang quantitative PCR instrument, but also comprises 16-hole and 32-hole small-flux fluorescent quantitative PCR instruments; the filter is a narrow-band filter suitable for a wavelength range of 480-520nm, the span range is small, and the specific calibration of the wavelength range of a specific report fluorescent group can be realized; the temperature data collected by the probe and the optical data collected by the measured fluorescence quantitative PCR instrument are arranged by the calibration module, so that the accuracy and the reliability of the fluorescence quantitative PCR instrument can be effectively reflected.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments are briefly described below to form a part of the present utility model, and the exemplary embodiments of the present utility model and the description thereof illustrate the present utility model and do not constitute undue limitations of the present utility model; in the drawings:
FIG. 1 is a schematic structural diagram of a technical scheme of a calibrating device of a fluorescent quantitative PCR apparatus according to an embodiment of the present utility model;
FIG. 2 is a perspective view of an embodiment of the present utility model;
fig. 3 is an exploded view of an embodiment of the present utility model.
In the figure:
a host 1; a light transmitting hole 11; a socket 12; a circuit board 13; a main body case 14; a groove 15; a filter 2; an LED light source 3; a bolt assembly 4; a temperature probe 5; a middle probe 51; a left probe 52; a right probe 53; a cylinder 500; a conical end 510; a connecting piece 6; a screw 7; and a wire 8.
Detailed Description
In order to make the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to specific embodiments of the present utility model and corresponding drawings. In the description of the present utility model, it should be noted that, as used in the specification and the claims, the term "comprising" is an open-ended term, and thus should be interpreted to mean "including, but not limited to"; "number" means more than 2. In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
As shown in fig. 1-3, the technical scheme of the utility model comprises:
the calibrating device of the fluorescent quantitative PCR instrument is used for calibrating the temperature and the optics of the fluorescent quantitative PCR instrument and comprises a host machine 1 provided with a circuit board 13 and three identical calibrating modules which are respectively and fixedly arranged on the host machine 1 in a triangular shape, wherein each calibrating module comprises a temperature calibrating unit and an optics calibrating unit; wherein,,
the top wall of the outer shell 14 arranged on the host 1 is provided with light holes 11 respectively positioned at the vertices of the triangle, the center of the light holes 11 positioned in the middle is positioned on the Y-direction central line of the outer shell 14, and the bottom of the outer shell 14 is provided with the circuit board 13.
The temperature calibration unit comprises a temperature probe 5, wherein the temperature probe 5 comprises a middle probe 51 positioned in the middle, a left probe 52 and a right probe 53 positioned at two sides, and the temperature probe is respectively fixedly arranged on the outer side of the circuit board 13 and positioned at the vertex of the triangle, is connected with the circuit board 13 through a lead 8 and is used for measuring temperature data and optical data of middle and edge zones.
The optical calibration unit comprises an LED light source 3 which is arranged in the circuit board 13 and projects light to the light transmission hole 11, and a light filter 2 is arranged between the LED light source 3 and the top wall.
The preferred embodiment of the present utility model is shown in fig. 1-3:
the utility model provides a calibrating device of fluorescence ration PCR appearance for right fluorescence ration PCR appearance temperature and optical calibration, is including being equipped with host computer 1 and being triangle-shaped of circuit board 13 and being respectively through bolt assembly 4 and screw 7 solid dress three the same calibration module on the host computer 1, the calibration module all includes temperature calibration unit and optical calibration unit, wherein, the roof of the shell body 14 that host computer 1 was equipped with is located respectively light trap 11 of triangle-shaped summit department, and be located the centre light trap 11's center is in on the Y to the central line of shell body 14, the bottom of shell body 14 is equipped with circuit board 13.
The temperature calibration unit comprises temperature probes 5, wherein the temperature probes 5 are respectively fixedly arranged on the outer side of the circuit board 13 through screws 7 and positioned at the vertex of the triangle, and comprise a middle probe 51 positioned in the middle, a left probe 52 and a right probe 53 positioned on two sides, and are connected with the circuit board 13 through wires 8 for measuring temperature data and optical data of middle and edge zones.
The optical calibration unit comprises a light hole 11 and three LED light sources 3 which are arranged in a measuring groove 15 in the circuit board 13 and throw light rays to the light hole 11, the upper ends of the LED light sources 3 are positioned on the same horizontal plane, and a light filter 2 is arranged between the LED light sources 3 and the top wall.
The vertical centers of the light holes 11 at the vertex of each triangle, the LED light source 3 and the temperature probe 5 are all coincident.
In this example, the outer casing 14 is a square body, the upper end is provided with a top wall, the lower end is provided with a groove 15, the side wall of the groove 15 is provided with a socket 12, the light-transmitting hole 11 is a through hole, the inner diameter of the light-transmitting hole 11 is equal to the diameter of a reaction hole of a temperature control module provided by the PCR instrument to be tested, and the positions of the three light-transmitting holes 11 correspond to the three vertexes of the triangle; the top wall and the light holes 11 are square, the corresponding sides are parallel to each other, one light hole 11 positioned in the middle position is positioned on the Y-direction central line, the other two light holes 11 are symmetrically arranged by taking the Y-direction central line as the central line, the simultaneous calibration of the center and the edge is realized, the distance between the center of the light hole 11 and the edge of the outer shell 14 is not less than 9cm, the phenomenon that the acquired data is incomplete due to the edge effect of the fluorescent quantitative PCR instrument is effectively avoided, and the light hole is not only suitable for the physical detection of the 96 Kong Yingguang quantitative PCR instrument and the 48 Kong Yingguang quantitative PCR instrument, but also comprises the small-flux fluorescent quantitative PCR instrument with 16 holes, 32 holes and the like.
In this example, the LED light sources are welded on the circuit board 13, the planes of the upper ends of the three LED light sources 3 for supporting the optical filters 2 are in the same horizontal plane, the optical filters 2 are placed from the insertion openings 12 into the optical filters 2, the optical filters 2 are narrow-band optical filters and are placed between the LED light sources 3 and the top wall, the narrow-band optical filters are applicable to a wavelength range of 480-520nm, the narrow-band optical filters are applicable to a small wavelength span range, and can be calibrated for a single fluorescent probe or fluorescent dye, so that the specific calibration for the wavelength range of a specific reporting fluorescent group can be realized.
In this example, the temperature probe 5 is vertically downward and is perpendicular to the circuit board 13, the temperature probe 5 includes a cylinder 500 located at the upper portion and a conical end 510 at the lower end of the cylinder 500, a wire 8 is disposed in the inner cavity, the wire 8 is welded with the circuit board 13, the cylinder 500 and the conical end 510 of the temperature probe 5 are matched and attached to a measured temperature control module measuring hole of the PCR instrument, temperature measurement data are transmitted to a computer (not shown in the figure) through the circuit board 13, a connecting piece 6 is fixedly welded at the upper end of the temperature probe 5, and the connecting piece 6 is fixedly connected with the circuit board 13 through a screw 7.
The accuracy and the reliability of the fluorescent quantitative PCR instrument can be effectively reflected through the calibration module arranged on the calibration device of the fluorescent quantitative PCR instrument, namely, the temperature data acquired by the temperature probe 5 and the optical data acquired by the measured fluorescent quantitative PCR instrument.
The embodiments of the present utility model have been described above with reference to the accompanying drawings, but the present utility model is not limited to the above-described embodiments, which are merely illustrative and not restrictive, and many forms may be made by those having ordinary skill in the art without departing from the spirit of the present utility model and the scope of the claims, which are to be protected by the present utility model.
Claims (8)
1. A calibrating device of a fluorescent quantitative PCR instrument, which is used for calibrating the temperature and optics of the fluorescent quantitative PCR instrument, and is characterized in that: the device comprises a host machine provided with a circuit board and three identical calibration modules which are respectively and fixedly arranged on the host machine in a triangle shape, wherein each calibration module comprises a temperature calibration unit and an optical calibration unit; wherein,,
the top wall of the outer shell body arranged on the host is provided with light holes respectively positioned at the vertexes of the triangles, the center of the light holes positioned in the middle is positioned on the Y-direction central line of the outer shell body, and the bottom of the outer shell body is provided with the circuit board;
the temperature calibration unit comprises temperature probes which are respectively fixedly connected to the outer side of the circuit board and positioned at the vertex of the triangle;
the optical calibration unit comprises a light source which is arranged on the inner side of the circuit board and projects light to the light transmission hole, and a light filter is arranged between the light source and the top wall.
2. The calibration device of a fluorescent quantitative PCR instrument according to claim 1, wherein: the outer shell is square, the light holes are square holes, the edges of the top wall are parallel to the corresponding edges of the square holes, and the center distance of the light holes is not smaller than 9cm from the edge of the outer shell.
3. The calibration device of a fluorescent quantitative PCR instrument according to claim 2, wherein: the center of the light holes in the middle is on the Y-direction central line, and the other two light holes are symmetrically arranged by taking the Y-direction central line as a central line.
4. The calibration device for a fluorescent quantitative PCR instrument according to claim 3, wherein: the inner diameter of the light hole is equal to the diameter of a reaction hole of a temperature control module arranged on the PCR instrument to be tested.
5. The calibration device of a fluorescent quantitative PCR instrument according to claim 1, wherein: the light source comprises an LED light source, and the LED light source is welded on the circuit board.
6. The calibration device for a fluorescent quantitative PCR apparatus according to claim 5, wherein: the optical filter is horizontally arranged on the LED light source, the optical filter is a narrow-band optical filter, and the narrow-band optical filter is applicable to a wavelength range of 480-520 nm.
7. The calibration device of a fluorescent quantitative PCR instrument according to claim 1, wherein: the temperature probe is downward arranged and perpendicular to the circuit board, the temperature probe comprises a cylinder positioned on the upper part and a cone positioned at the lower end part of the cylinder, and the temperature probe is matched and attached with a measured temperature control module measuring hole of the PCR instrument.
8. The calibration device for a fluorescent quantitative PCR instrument according to claim 7, wherein: the temperature probe is fixedly connected with the circuit board through a connecting sheet fixedly arranged at the upper end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320166407.6U CN219239661U (en) | 2023-02-09 | 2023-02-09 | Calibrating device of fluorescence quantitative PCR instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320166407.6U CN219239661U (en) | 2023-02-09 | 2023-02-09 | Calibrating device of fluorescence quantitative PCR instrument |
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| Publication Number | Publication Date |
|---|---|
| CN219239661U true CN219239661U (en) | 2023-06-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320166407.6U Active CN219239661U (en) | 2023-02-09 | 2023-02-09 | Calibrating device of fluorescence quantitative PCR instrument |
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2023
- 2023-02-09 CN CN202320166407.6U patent/CN219239661U/en active Active
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