CN111007044A - Optical fiber module for fluorescence detection and fluorescence detector - Google Patents
Optical fiber module for fluorescence detection and fluorescence detector Download PDFInfo
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- CN111007044A CN111007044A CN201911070418.9A CN201911070418A CN111007044A CN 111007044 A CN111007044 A CN 111007044A CN 201911070418 A CN201911070418 A CN 201911070418A CN 111007044 A CN111007044 A CN 111007044A
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- optical fiber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
Abstract
The invention relates to the field of experimental instruments, in particular to an optical fiber module and a fluorescence detector for fluorescence detection, which comprise an optical fiber fixing plate, a light source and a fluorescence receiving optical fiber, wherein the surface of the optical fiber fixing plate is provided with a plurality of optical fiber excitation ends, the emitted light of the light source is irradiated on a sample to be detected by the optical fiber excitation ends to generate fluorescence, and the fluorescence receiving optical fiber is used for receiving the generated fluorescence; the fluorescence receiving optical fiber is also provided with a data connecting end; the spacing between the fiber excitation ends is the same as that between the eight connecting pipes. The invention adopts a plurality of optical fiber excitation ends fixed on the optical fiber fixing plate and matched with the eight-connected tube for use, can realize simultaneous detection of samples of the eight-connected tube, realizes one-time rapid detection, improves the repeatability and stability of detection, and facilitates splicing and combination or interchange of modularized optical fiber modules according to requirements. In addition, the phenomenon of position deviation of the optical fiber excitation end caused by frequent movement is avoided, and the maintenance cost of equipment is reduced.
Description
Technical Field
The invention relates to the field of experimental instruments, in particular to an optical fiber module for fluorescence detection and a fluorescence detector.
Background
The newly developed isothermal nucleic acid amplification technology is simpler and more convenient than the PCR technology in terms of actual operation and instrument requirements. Isothermal amplification techniques generally utilize the change of absorption or fluorescence signals with time after light irradiation of a solution to perform qualitative or quantitative analysis on a sample to be detected in the solution. At present, a fluorescence detector which is convenient to carry and operate has been developed in the prior art, the small detector is often matched with an eight-connecting pipe for use, and the detector is provided with an optical fiber excitation end which is driven by a crawler belt or other transmission parts. After one sample is detected, the fiber laser is moved to detect the next sample. When more samples need to be tested, eight tubes need to be replaced several times. In the process of waiting for testing, the sample to be tested may change, and the repeatability of the test result is not good. Moreover, the position of the optical fiber excitation end is frequently changed, so that the position of the optical fiber excitation end is possibly deviated, the accuracy of the test is influenced, and the maintenance cost is increased.
Disclosure of Invention
The invention aims to solve the problems of poor repeatability and low accuracy of a test result of a fluorescence detector in the prior art, and provides an optical fiber module for fluorescence detection.
Another object of the present invention is to provide a fluorescence detector including the above optical fiber module for fluorescence detection.
The purpose of the invention is realized by the following technical scheme:
an optical fiber module for fluorescence detection comprises an optical fiber fixing plate, a light source and a fluorescence receiving optical fiber, wherein a plurality of optical fiber excitation ends are arranged on the surface of the optical fiber fixing plate, the distance between the plurality of optical fiber excitation ends and the distance between eight connecting pipes are the same, the emitted light of the light source is irradiated to a sample to be detected by the optical fiber excitation ends to generate fluorescence, and the fluorescence receiving optical fiber is used for receiving the generated fluorescence; the fluorescence receiving optical fiber is also provided with a data connecting end.
The fiber optic modules described above for fluorescence detection are typically used in conjunction with eight tubes. The space between the optical fiber excitation ends is the same as that of the eight-connected pipe, the optical fiber excitation ends are fixed on the optical fiber fixing plates, one fixing plate is provided with 8 optical fiber excitation ends or multiple optical fiber excitation ends of 8, and each optical fiber excitation end corresponds to the eight-connected pipe. The fixing plate is provided with a fixed mounting hole, and the optical fiber excitation end is fixedly arranged in the mounting hole. The fixing plate is provided with a light source, the emitted light of the light source irradiates the sample to be tested through the optical fiber excitation end to generate fluorescence, and then the fluorescence receiving optical fiber transmits the fluorescence signal to the data processing module to complete the fluorescence test. By the arrangement, on one hand, the simultaneous and one-time detection of 8 samples or more samples can be realized, the detection time is saved, and the repeatability and the accuracy of the detection are improved; in addition, the optical fiber excitation end does not generate the phenomenon of position deviation because of frequent movement, the testing accuracy is improved, and the maintenance cost of the equipment is reduced.
The light emitted by the light source irradiates the sample to be measured through the optical fiber excitation end to generate fluorescence. There are various ways to arrange the light source, and the following two ways are provided in the present invention.
The optical fiber fixing plate is characterized in that a plurality of optical fiber excitation ends are arranged on one side of the optical fiber fixing plate, light sources are arranged on the other side of the optical fiber fixing plate, the optical fiber excitation ends and the light sources are arranged in a one-to-one correspondence mode, and each optical fiber excitation end is also connected with a fluorescence receiving optical fiber; the fluorescence receiving optical fiber is connected with the data connecting end. The light source in this way preferably uses a light source column.
The light source may be provided in a plurality, preferably 8 or 8 times, each light source corresponding to one fiber excitation end. However, the length of the fixing plate is about 56-60 mm because the distance between the two eight pipes is about 9 mm. The 8 light sources are fixed on the short fixing plate, so that the process is complex and the operation is not easy, and the light sources are easy to influence each other.
In another arrangement of the light source, the light source is arranged in a cassette, and the cassette is connected with each optical fiber excitation end through an incident optical fiber.
Preferably, a plurality of optical fiber interfaces are arranged on the cassette; one end of the incident optical fiber is connected with the optical fiber excitation end through a plurality of incident optical fiber branches, and the other end of the incident optical fiber is provided with an optical fiber joint; each incident optical fiber branch is in one-to-one correspondence with each optical fiber excitation end, and the optical fiber interface is connected with the optical fiber interface.
In order to solve the problems that light sources generated by multiple light sources are difficult to fix and the light sources are difficult to influence each other, a cassette is additionally arranged on a module, and a plurality of optical fiber interfaces are arranged on the cassette. The fiber excitation end and the cassette are connected through an incident fiber. And a plurality of incident optical fibers are integrated into an optical fiber bundle, the optical fiber bundle is connected with the optical fiber connector, and then the optical fiber connector is connected with the optical fiber interface on the cassette. The optical fiber connector and the optical fiber interface adopt a connection mode of threads and nuts, and the optical fiber connector is provided with a gasket to ensure the light tightness of the cassette.
Preferably, the light source is a linear light source. The light source is arranged in the cassette, preferably the light source is a linear optical fiber, and the optical fiber interface on the cassette is distributed in parallel with the linear light source. The linear light source can ensure that the light intensity and wavelength at each fiber interface are consistent. The repeatability and stability of the experiment can be improved.
Preferably, the optical fiber interface is provided with an optical filter. An optical filter is further arranged at the optical fiber interface, and incident light with specific wavelength is selected according to needs, so that the generation of a fluorescence effect is facilitated.
Preferably, the optical fiber interface is further provided with a collimator. And the collimator is additionally arranged, so that exciting light can be converted into parallel light, and the utilization rate of the exciting light is increased.
Preferably, the light source is an LED light source.
The fluorescence detector comprises the optical fiber module and a data processing module, wherein the data processing module is provided with a plurality of data connecting ports, and the data connecting ports are matched with the data connecting ends.
The fluorescence detector comprises the optical fiber module and the data processing module, wherein the data processing module is provided with a plurality of data interfaces, and a user can connect a plurality of optical fiber modules in parallel as required. The data connection end on the optical fiber module is connected with the data interface, and the fluorescence signal obtained by the optical fiber module is transmitted to the data processing module by the fluorescence receiving optical fiber, so that a plurality of samples can be rapidly detected simultaneously.
Compared with the prior art, the invention has the following technical effects:
according to the optical fiber module and the fluorescence detector for fluorescence detection, the plurality of optical fiber excitation ends are fixed on the optical fiber fixing plate and are matched with the eight-connected tubes for use, so that samples of the eight-connected tubes can be detected simultaneously, one-time rapid detection is realized, and the repeatability and the stability of detection are improved. In addition, the modularized optical fiber modules are convenient to splice and combine or exchange according to needs, namely a plurality of optical fiber modules can be connected in parallel according to needs to realize simultaneous detection of more samples, and detection results with better repeatability can be obtained when the same sample is subjected to parallel test.
Drawings
FIG. 1 is a schematic perspective view (front view) of a fluorescence detection module according to embodiment 1 of the present invention;
FIG. 2 is a schematic perspective view (back side) of a fluorescence detection module according to embodiment 1 of the present invention;
FIG. 3 is a schematic perspective view (front view) of a fluorescence detection module according to embodiment 2 of the present invention;
FIG. 4 is a schematic perspective view (back side) of a fluorescence detection module according to embodiment 2 of the present invention;
description of reference numerals:
1-optical fiber fixing plate, 2-optical fiber excitation end, 3-light source, 4-fluorescence receiving optical fiber, 41-data connecting end, 5-cassette, 51-optical fiber interface, 6-incident optical fiber, 61-optical fiber joint and 62-optical fiber branch.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Example 1
As shown in fig. 1 and 2, an optical fiber module for fluorescence detection includes an optical fiber fixing plate 1, a plurality of optical fiber excitation ends 2 are disposed on a surface of the optical fiber fixing plate 1, the optical fiber fixing plate 1 is provided with a light source 3, the optical fiber excitation ends 2 are disposed corresponding to the light source 3, and each optical fiber excitation end 2 is connected to a fluorescence receiving optical fiber 4; the fluorescence receiving fiber 4 is also provided with a data connection 41. The space between the optical fiber excitation ends 2 is the same as that between the eight connecting pipes, and the space is 9 mm.
The light source 3 is a light source column, and the light source column and the light excitation end 2 are respectively positioned at two sides of the optical fiber fixing plate 1.
The light source 3 is an LED light source, and a light filter and a collimator are arranged at the light source. The optical filter and the collimator are arranged between the light source 3 and the optical fiber excitation end 2, and the arranged position relation is that the light source, the optical filter, the collimator and the optical fiber excitation end are arranged in sequence.
A fluorescence detector comprises an optical fiber module and a data processing module, wherein the data processing module is provided with a plurality of data connecting ports, and each data connecting port is matched with each data connecting end 41.
When the device is used, the optical fiber modules are connected in parallel according to needs, and the eight-connection pipe containing the sample to be detected is placed on the detector. The light emitted by different light sources passes through the optical filter and the collimator and then irradiates the sample through the excitation end of the optical fiber. The sample electron excited transition emits fluorescence in the process of returning to the ground state, and the fluorescence is transmitted to the data processing module through the fluorescence receiving optical fiber under the action of the optical element in the optical fiber exciting end. The fluorescence signal is processed by the data processing module to obtain a test result. Because each eight-connecting pipe corresponds to one fluorescence excitation end and the fluorescence receiving optical fiber, the simultaneous detection of a plurality of groups of samples is realized, and the consistency among holes of the detection result is better.
Example 2
As shown in fig. 3 and 4, an optical fiber module for fluorescence detection includes an optical fiber fixing plate 1, a plurality of optical fiber excitation ends 2 are disposed on a surface of the optical fiber fixing plate 1, the optical fiber module further includes a cassette 5, a light source 3 is disposed in the cassette 5, the cassette 5 is connected to the optical fiber excitation ends 2 through an incident optical fiber 6, and each optical fiber excitation end 2 is connected to a fluorescence receiving optical fiber 4; the fluorescence receiving fiber 4 is also provided with a data connection 41. The space between the optical fiber excitation ends 2 is the same as that between the eight connecting pipes, and the space is 9 mm.
The cassette 5 is provided with a plurality of optical fiber interfaces 51; one end of the incident optical fiber 6 is connected with the optical fiber excitation end 2 through an incident optical fiber branch 62, and the other end is provided with an optical fiber connector 61. The optical fiber connector 61 is connected to the optical fiber interface 51.
The light source 3 (not shown in the figure) is a linear light source. The optical fiber interface 51 is provided with an optical filter. The fiber interface 51 is also provided with a collimator. The light source 3 is an LED light source. The optical filter and the collimator are arranged between the light source 3 and the optical fiber interface 5, and the arranged position relation is that the light source, the optical filter, the collimator and the optical fiber interface are sequentially arranged.
The fluorescence detector comprises the optical fiber module and a data processing module, wherein the data processing module is provided with a plurality of data connecting ports, and the data connecting ports are matched with the data connecting end 41.
When the device is used, the optical fiber modules are connected in parallel according to needs, and the eight-connection pipe containing the sample to be detected is placed on the detector. The light emitted by the light source passes through the filter and the collimator and is transmitted by the incident optical fiber to irradiate on the sample through the optical fiber excitation end. The sample electron excited transition emits fluorescence in the process of returning to the ground state, and the fluorescence is transmitted to the data processing module through the fluorescence receiving optical fiber under the action of the optical element in the optical fiber exciting end. The fluorescence signal is processed by the data processing module to obtain a test result. Because each eight-connecting pipe corresponds to one fluorescence excitation end and the fluorescence receiving optical fiber, the simultaneous detection of a plurality of groups of samples is realized, and the consistency among holes of the detection result is better.
Examples of the experiments
For the Sodium Fluorescein (Fluorescein Sodium) contained, the molecular formula isC20H10Na2O5Fluorescence detection was carried out using a 3.3 ng/. mu.L solution. And (5) transferring the solution to be detected, and putting the solution into different holes of the eight tubes. Eight-connected pipes are marked with A1 holes and A2 holes, … … holes and 8 holes. The constant temperature amplification fluorescence detector Deaou-308C and the fluorescence detector of the invention are adopted for respective tests, and the test results are shown in Table 1.
TABLE 1 samples the results should be tested
CV value is a discrete coefficient, and CV ═ sd/mean × 100% (sd is the standard deviation of a set of data, mean is the average of a set of data).
As can be seen from Table 1, when fluorescence tests are performed on samples in the same eight-connected tube, the consistency among the holes of the results measured by a conventional fluorescence detector is 6-7%; the consistency among the holes of the result measured by the fluorescence detector is 2-2.5%, which shows that the optical fiber module adopted by the fluorescence detector can be used for testing different holes simultaneously, and the obtained test result has better consistency. In addition, when multiple parallel tests are carried out, the repeatability of the test result of the conventional detector is 1.2-1.8%; the repeatability of the test result of the detector is 0.1-0.5%, which shows that the invention realizes the simultaneous detection of a plurality of groups of parallel test groups by connecting a plurality of optical fiber modules in parallel, and the obtained test result has better repeatability.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or the orientations or positional relationships in which the products of the present invention are conventionally placed when used, and are used only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical", "suspended", and the like do not imply that the components are required to be absolutely horizontal or suspended, but rather may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it is also to be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The drawings of the embodiments of the disclosure only relate to the structures related to the embodiments of the disclosure, and other structures can refer to the common design.
For purposes of clarity, the thickness of layers or regions in the figures used to describe embodiments of the present disclosure are exaggerated or reduced, i.e., the figures are not drawn on a true scale. Without conflict, embodiments of the present disclosure and features of the embodiments may be combined with each other to arrive at new embodiments.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims (9)
1. The optical fiber module for fluorescence detection is characterized by comprising an optical fiber fixing plate (1), a light source (3) and a fluorescence receiving optical fiber (4), wherein a plurality of optical fiber excitation ends (2) are arranged on the surface of the optical fiber fixing plate (1), the distance between the optical fiber excitation ends (2) is the same as that between eight connecting pipes, the emitted light of the light source (3) is irradiated to a sample to be detected by the optical fiber excitation ends (2) to generate fluorescence, and the fluorescence receiving optical fiber (4) is used for receiving the generated fluorescence; the fluorescence receiving optical fiber (4) is also provided with a data connecting end (41).
2. The optical fiber module for fluorescence detection according to claim 1, wherein a plurality of optical fiber excitation ends (2) are disposed on one side of the optical fiber fixing plate (1), a light source (3) is disposed on the other side of the optical fiber fixing plate, the optical fiber excitation ends (2) and the light source (3) are disposed in a one-to-one correspondence, and each optical fiber excitation end (2) is further connected to a fluorescence receiving optical fiber (4).
3. The fiber optic module for fluorescence detection of claim 1, wherein the light source (3) is disposed within a cassette (5), the cassette (5) being connected to each of the fiber excitation ends (2) by an incident fiber (6).
4. The fiber optic module for fluorescence detection according to claim 3, wherein the cassette (5) is provided with a plurality of fiber optic interfaces (51); one end of the incident optical fiber (6) is connected with the optical fiber excitation end (2) through a plurality of incident optical fiber branches (62), and the other end of the incident optical fiber is provided with an optical fiber joint (61); each incident optical fiber branch (62) and each optical fiber excitation end (2) are arranged in a one-to-one correspondence mode, and the optical fiber connectors (61) are connected with the optical fiber interfaces (51).
5. The fiber optic module for fluorescence detection of claim 4, wherein the fiber optic interface (51) is provided with an optical filter.
6. The fiber optic module for fluorescence detection of claim 5, wherein the fiber optic interface (51) is further provided with a collimator.
7. The fiber optic module for fluorescence detection of claim 6, wherein the light source (3) is a linear light source.
8. The fiber optic module for fluorescence detection of claim 7, wherein the light source (3) is an LED light source.
9. A fluorescence detector, comprising an optical fiber module according to any one of claims 1 to 8 and a data processing module, wherein the data processing module is provided with a plurality of data connection ports, and the data connection ports are matched with the data connection ports (41).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112823890A (en) * | 2020-07-30 | 2021-05-21 | 北京金诺美生物技术有限公司 | Real-time fluorescence quantitative PCR instrument |
| CN113155801A (en) * | 2021-05-10 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Fluorescence detector |
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Application publication date: 20200414 |
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