CN219079456U

CN219079456U - Automatic library construction and sample addition system for library construction instrument

Info

Publication number: CN219079456U
Application number: CN202223451852.1U
Authority: CN
Inventors: 皇福详; 沈洪飞; 潘林伟; 宋安琦; 范兆飞; 顾志鹏; 王春明
Original assignee: Xiamen Deyun Xinzhun Technology Co ltd
Current assignee: Xiamen Deyun Xinzhun Technology Co ltd
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-05-26
Anticipated expiration: 2032-12-22

Abstract

The utility model discloses an automatic library construction and sample addition system for a library construction instrument. The system comprises an X-axis movement module, a Y-axis movement module, a Z-axis movement module, a suction nozzle gun rod and an injection pump, wherein the Y-axis movement module is slidably mounted on the X-axis movement module, the Z-axis movement module is slidably mounted on the Y-axis movement module, the Z-axis movement module is provided with a Z-axis mounting block, the upper end of the suction nozzle gun rod is slidably mounted on the Z-axis movement module, the lower end of the suction nozzle gun rod is provided with an expanding part, the middle end of the suction nozzle gun rod is externally sleeved with an ejection block, the top of the ejection block is provided with a vertical spring baffle column, and the top of the suction nozzle gun rod is connected with the injection pump. The automatic library construction and sample addition system for the library construction instrument can be used for adding various reagents required by cell lysis into a chip according to a certain sequence and a certain time difference in the operation of the library construction instrument, and can automatically replace a suction head, so that time and labor are saved, and cross contamination is reduced.

Description

Automatic library construction and sample addition system for library construction instrument

Technical Field

The utility model relates to the field of medical instruments, in particular to an automatic library construction and sample addition system for a library construction instrument.

Background

In the biological experiment of sequencing and analyzing cell nucleic acid, cell suspension is required to be injected into a special chip, then the cell suspension is settled into micropores of the chip, each micropore contains a cell and a microsphere, and then the subsequent steps of accurate sample adding, cleaning, cracking and the like, namely steps of capturing, cracking and RNA capturing of the cell in the sequencing process are carried out by matching with corresponding instruments, so that the sequencing and analyzing of genome, transcriptome, epigenome, proteome and the like are carried out on the single cell level.

In the whole sequencing analysis process, various operations such as sample loading, standing, shaking and the like need to be performed on a sample for many times, the whole process is complicated, if the operations are performed manually, time and labor are wasted, errors are often high, and the final analysis result is easily affected, so that in actual operation, equipment capable of automatically completing the operations, namely a library constructor, needs to be used.

In the library construction instrument, a mechanism for providing sample materials can provide a plurality of suckers and a plurality of reagents at a time, each suckers and reagents need to be encoded through system control, and in the experimental process, various stock solutions or reagents including cell suspension, microsphere suspension, lysis buffer, mineral oil, PBS and the like need to be added to a sample of a chip in batches, and the addition needs to be performed according to a certain sequence and a certain time difference, wherein according to different system flows, 6-12 suckers are used at a time, and the suckers after use need to be replaced in time, so that a special sample adding system is needed to complete.

Disclosure of Invention

In order to solve the problems, the utility model provides an automatic library construction and sample addition system for a library construction instrument.

According to one aspect of the present utility model, there is provided an automated library construction and sample addition system for a library construction instrument, comprising an X-axis movement module, a Y-axis movement module, a Z-axis movement module, a tip stock, and a syringe pump, wherein the Y-axis movement module is slidably mounted on the X-axis movement module, the Z-axis movement module is slidably mounted on the Y-axis movement module, and the Z-axis movement module has a Z-axis mounting block; the upper end of the suction head gun rod is slidably arranged on the Z-axis movement module, the lower end of the suction head gun rod is provided with an expansion part, an ejection block is sleeved outside the middle end of the suction head gun rod, a vertical spring baffle column is arranged at the top of the ejection block, and the Z-axis installation block extends to the position right above the spring baffle column; the top end of the suction head gun rod is connected with the injection pump, and the bottom end of the suction head gun rod is used for installing the suction head.

In some embodiments, the X-axis motion module comprises a chassis, an X-axis screw motor and an X-axis slide rail, the X-axis screw motor and the X-axis slide rail are both mounted on the chassis, and the Y-axis motion module is connected to the X-axis screw motor and slidably mounted on the X-axis slide rail. The X-axis motion module has the advantage that the structure of the X-axis motion module is described.

In some embodiments, the Y-axis motion module comprises a Y-axis mounting block, a Y-axis screw motor and two parallel Y-axis sliding rails, wherein the Y-axis mounting block is connected to the X-axis screw motor and slidably mounted on the X-axis sliding rails, the Y-axis screw motor and the two Y-axis sliding rails are both mounted on the Y-axis mounting block, and the Z-axis mounting block is connected to the Y-axis screw motor and slidably mounted on the two Y-axis sliding rails. The Y-axis motion module has the advantage that the structure of the Y-axis motion module is described.

In some embodiments, the Z-axis motion module further comprises a Z-axis lead screw motor and a Z-axis slide rail, and the suction nozzle gun rod is connected to the Z-axis lead screw motor and slidably mounted on the Z-axis slide rail. The Z-axis motion module has the advantage that the structure of the Z-axis motion module is described.

In some embodiments, the suction head holster is connected to the Z-axis lead screw motor and slidably mounted on the Z-axis slide rail by a holster mounting block, and the spring catch is slidably connected to the holster mounting block and extends above the holster mounting block. The advantage is that the relevant structure of the suction nozzle gun shaft is further described.

In some embodiments, the chassis, the Y-axis mounting block and the Z-axis mounting block are each provided with an optocoupler switch, and the Y-axis mounting block, the Z-axis mounting block and the gun rod mounting block are each provided with an optocoupler blocking piece corresponding to each optocoupler switch. The optical coupler has the advantages that the closed loop feedback can be formed by arranging the optical coupler switches and the optical coupler baffle plates, and the accurate positioning is realized.

Drawings

FIG. 1 is a schematic diagram of an automated library construction and loading system for a library construction instrument according to one embodiment of the present utility model in use;

FIG. 2 is a schematic structural diagram of the X-axis motion module shown in FIG. 1;

FIG. 3 is a schematic diagram of the Y-axis motion module shown in FIG. 1;

fig. 4 is a schematic diagram of the structure of the Z-axis motion module shown in fig. 1 and the suction nozzle gun shaft.

In the figure: x-axis motion module 1, Y-axis motion module 2, Z-axis motion module 3, suction nozzle gun rod 4, injection pump 5, suction nozzle 6, opto-coupler switch 7, consumable platform 8, chip 9, chassis 11, X-axis lead screw motor 12, X-axis slide rail 13, Y-axis installation block 21, Y-axis lead screw motor 22, Y-axis slide rail 23, Z-axis installation block 31, Z-axis lead screw motor 32, Z-axis slide rail 33, expansion part 41, ejection block 42, spring baffle column 43, gun rod installation block 44, opto-coupler baffle 71.

Detailed Description

The utility model is described in further detail below with reference to the accompanying drawings.

Fig. 1 schematically shows the structure of an automated banking and loading system for a library construction machine in use, fig. 2 shows the structure of the X-axis movement module of fig. 1, fig. 3 shows the structure of the Y-axis movement module of fig. 1, and fig. 4 shows the structure of the Z-axis movement module of fig. 1 and the associated structure of the tip stick, according to one embodiment of the present utility model.

As shown in fig. 1, the library-building and sample-adding system mainly comprises an X-axis movement module 1, a Y-axis movement module 2, a Z-axis movement module 3, a suction nozzle gun rod 4 and a syringe pump 5, wherein the approximate positional relationship of each mechanism is as follows: the Y-axis movement module 2 is slidably mounted on the X-axis movement module 1, the Z-axis movement module 3 is slidably mounted on the Y-axis movement module 2, the suction nozzle gun rod 4 is slidably mounted on the Z-axis movement module 3, the top end of the suction nozzle gun rod 4 is connected with the injection pump 5, and the suction nozzle 6 can be mounted at the bottom end of the suction nozzle gun rod 4.

This build storehouse application of sample system needs to cooperate consumable platform 8 and chip 9 (omit its installation and removal structure in the figure) to use, and wherein can stably place a plurality of suction heads 6 and many test tubes that hold reagent and stoste on the consumable platform 8 to still design a waste tank, and chip 9 is the reaction place that is used for carrying out the sequencing experiment. Wherein, can be with application of sample system, consumable platform 8 and chip 9 together constitute a library construction appearance that can automatic operation.

As shown in fig. 2, the X-axis motion module 1 mainly includes a chassis 11, an X-axis screw motor 12 and an X-axis slide rail 13, and the X-axis screw motor 12 and the X-axis slide rail 13 are both mounted on the chassis 11. Wherein, the Y-axis motion module 2 is connected to the screw of the X-axis screw motor 12, and the Y-axis motion module 2 is also slidably mounted on the X-axis slide rail 13, so that the X-axis screw motor 12 operates to drive the Y-axis motion module 2 and the mechanisms thereon to slide along the X-axis slide rail 13 in the X-axis direction.

As shown in fig. 3, the Y-axis moving module 2 mainly includes a Y-axis mounting block 21, a Y-axis screw motor 22 and two parallel Y-axis slide rails 23, the Y-axis mounting block 21 is directly connected to the X-axis screw motor 12 and slidably mounted on the X-axis slide rails 13, and the Y-axis screw motor 22 and the two Y-axis slide rails 23 are both mounted on the Y-axis mounting block 21. Wherein, the Z-axis movement module 3 is connected to the Y-axis screw motor 22, and the Z-axis movement module 3 is further slidably mounted on two Y-axis sliding rails 23, so that the Y-axis screw motor 22 can drive the Z-axis movement module 3 and each mechanism thereon to slide along the Y-axis sliding rails 23 in the Y-axis direction.

As shown in fig. 4, the Z-axis moving module 3 mainly includes a Z-axis mounting block 31, a Z-axis screw motor 32 and a Z-axis slide rail 33, the Z-axis mounting block 31 is directly connected to the Y-axis screw motor 22 and slidably mounted on the two Y-axis slide rails 23, and the Z-axis screw motor 32 and the Z-axis slide rail 33 are both mounted on the Z-axis mounting block 31. Wherein, suction nozzle gun rod 4 is connected on the lead screw of Z axle lead screw motor 32 and also slidable mounting is on Z axle slide rail 33, then Z axle lead screw motor 32 operation can drive suction nozzle gun rod 4 and relevant structure along Z axle slide rail 33 in the Z axle direction.

As shown in fig. 4, the suction nozzle stick 4 is hollow and vertically disposed, and the upper end of the suction nozzle stick 4 is fixedly mounted on a stick mounting block 44, and the stick mounting block 44 is directly connected to the Z-axis screw motor 32 and slidably mounted on the Z-axis slide rail 33. Wherein the top of the Z-axis mounting block 31 also extends directly above the suction nozzle stick 4.

The lower end of the suction nozzle gun rod 4 is provided with a protruding expansion part 41, the size of the expansion part 41 is slightly larger than the size of the top nozzle of the suction nozzle 6, when the suction nozzle gun rod 4 slides downwards along with the gun rod mounting block 44 and the lower end of the suction nozzle gun rod is pressed on the suction nozzle 6, the expansion part 41 is clamped at the top nozzle of the suction nozzle 6, so that the suction nozzle 6 is automatically and fixedly mounted on the suction nozzle gun rod 4, and the injection pump 5 can control the suction nozzle 6 to perform the actions of liquid suction, liquid discharge and the like through the suction nozzle gun rod 4 under the control of a system. In addition, in order to reduce the occurrence of residue, foaming, and the like during pipetting and draining, air may be injected prior to pipetting.

An ejector block 42 is also connected below the lance mounting block 44, the ejector block 42 having a hole of a size substantially corresponding to the enlarged portion 41 of the lower end of the suction nozzle lance 4 (i.e. the enlarged portion 41 alone may pass through the hole but may not pass through the enlarged portion 41 after the suction nozzle 6 is mounted thereon), and the suction nozzle lance 4 passing through the hole of the ejector block 42, i.e. the ejector block 42 is sleeved outside the suction nozzle lance 4 but not in contact with the suction nozzle lance 4.

A vertical spring blocking post 43 is fixedly mounted on the top of the ejector block 42, the spring blocking post 43 penetrates through the gun rod mounting block 44 and can slide relatively to the gun rod mounting block 44, so that the gun rod mounting block 44 is in sliding connection with the ejector block 42, and the top end of the spring blocking post 43 extends to the position above the gun rod mounting block 44 and is positioned right below the extending top of the Z-axis mounting block 31.

When the suction head gun rod 4 with the suction head 6 mounted thereon slides upwards along with the gun rod mounting block 44, the ejection block 42 and the spring baffle 43 also slide upwards along with the connected gun rod mounting block 44 until the spring baffle 43 contacts with the Z-axis mounting block 31 above the spring baffle 43, the ejection block 42 fixedly connected with the spring baffle 43 is blocked from sliding any more, and the suction head gun rod 4 continues to slide upwards along with the gun rod mounting block 44; then, because the expansion part 41 on the suction head gun rod 4 cannot pass through the hole on the ejection block 42 after the suction head 6 is installed, extrusion stress is generated between the expansion part and the ejection block 42, and the suction head 6 can be extruded and fallen; finally, the sucker gun rod 4 slides downwards along with the gun rod mounting block 44 to enable the lower end expansion part 41 to be reinstalled with a new sucker 6, thereby realizing the function of automatically changing sucker liquid.

In addition, as shown in fig. 2 to 4, one optocoupler switch 7 is disposed on each of the chassis 11, the Y-axis mounting block 21 and the Z-axis mounting block 31, and one optocoupler baffle 71 is disposed on each of the Y-axis mounting block 21, the Z-axis mounting block 31 and the gun bar mounting block 44, wherein each optocoupler baffle 71 corresponds to each optocoupler switch 7 and can form closed loop feedback when in contact, so that accurate positioning can be achieved in the movement of the X-axis, the Y-axis and the Z-axis, respectively.

When the warehouse-building sample-adding system is used, the operation of the X-axis movement module 1, the Y-axis movement module 2 and the Z-axis movement module 3 is controlled by the system, the suction nozzle gun rod 4 is firstly moved to the upper part of the suction nozzle 6 on the consumable platform 8, then the suction nozzle gun rod 4 is lowered to mount the suction nozzle 6 on the suction nozzle gun rod 4, and then the suction nozzle 6 is matched with the operation of the injection pump 5, so that various reagents can be sequentially sucked from the consumable platform 8 by using the suction nozzle 6 according to the preset sequence and time difference in the system, and then the reagents are moved and injected into the chip 9 for reaction, wherein each axis movement module can accurately control the position and the insertion depth of the suction nozzle 6, and ensure the controllability of liquid suction and liquid discharge.

When the suction head 6 needs to be replaced, the suction head 6 is moved to the upper part of the waste tank, the gun rod mounting block 44 slides upwards until the ejection block 42 extrudes the suction head 6 to be separated from the waste tank, and then a new suction head 6 is mounted to continue the experiment operation.

What has been described above is merely some embodiments of the present utility model. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit of the utility model.

Claims

1. An automatic library construction and sample addition system for a library construction instrument, which is characterized in that: the suction head gun comprises an X-axis movement module (1), a Y-axis movement module (2), a Z-axis movement module (3), a suction head gun rod (4) and a syringe pump (5), wherein the Y-axis movement module (2) is slidably mounted on the X-axis movement module (1), the Z-axis movement module (3) is slidably mounted on the Y-axis movement module (2), and the Z-axis movement module (3) is provided with a Z-axis mounting block (31);

the upper end of the suction head gun rod (4) is slidably mounted on the Z-axis movement module (3), the lower end of the suction head gun rod is provided with an expansion part (41), an ejection block (42) is sleeved outside the middle end of the suction head gun rod, a vertical spring baffle column (43) is mounted at the top of the ejection block (42), and the Z-axis mounting block (31) extends to the position right above the spring baffle column (43);

the top end of the suction head gun rod (4) is connected with the injection pump (5), and the bottom end is used for installing the suction head (6).

2. An automated library-building and sample-adding system for a library construction machine according to claim 1, wherein: the X-axis movement module (1) comprises an underframe (11), an X-axis screw motor (12) and an X-axis sliding rail (13), wherein the X-axis screw motor (12) and the X-axis sliding rail (13) are both arranged on the underframe (11), and the Y-axis movement module (2) is connected to the X-axis screw motor (12) and is slidably arranged on the X-axis sliding rail (13).

3. An automated library-building and sample-adding system for a library construction machine according to claim 2, wherein: the Y-axis motion module (2) comprises a Y-axis installation block (21), a Y-axis lead screw motor (22) and two Y-axis sliding rails (23) which are parallel to each other, wherein the Y-axis installation block (21) is connected to the X-axis lead screw motor (12) and is slidably installed on the X-axis sliding rails (13), the Y-axis lead screw motor (22) and the two Y-axis sliding rails (23) are both installed on the Y-axis installation block (21), and the Z-axis installation block (31) is connected to the Y-axis lead screw motor (22) and is slidably installed on the two Y-axis sliding rails (23).

4. An automated library-building and sample-adding system for a library construction machine according to claim 3, wherein: the Z-axis movement module (3) further comprises a Z-axis screw motor (32) and a Z-axis sliding rail (33), and the suction nozzle gun rod (4) is connected to the Z-axis screw motor (32) and is slidably mounted on the Z-axis sliding rail (33).

5. An automated library addition system for a library construction machine according to claim 4, wherein: the suction head gun rod (4) is connected to the Z-axis screw motor (32) through a gun rod mounting block (44) and is slidably mounted on the Z-axis sliding rail (33), and the spring baffle column (43) is slidably connected to the gun rod mounting block (44) and extends to the upper side of the gun rod mounting block (44).

6. An automated library-building and sample-adding system for a library construction machine according to claim 5, wherein: the novel gun is characterized in that an optical coupler switch (7) is arranged on the underframe (11), the Y-axis installation block (21) and the Z-axis installation block (31), and optical coupler blocking pieces (71) corresponding to the optical coupler switches (7) are arranged on the Y-axis installation block (21), the Z-axis installation block (31) and the gun rod installation block (44).