CN218851674U

CN218851674U - Insect behavior observation device

Info

Publication number: CN218851674U
Application number: CN202221946096.7U
Authority: CN
Inventors: 林文汉; 张利荷; 王静; 周雨彤; 邱宝利
Original assignee: Guangdong Provincial Laboratory Of Lingnan Modern Agricultural Science And Technology; South China Agricultural University
Current assignee: Guangdong Provincial Laboratory Of Lingnan Modern Agricultural Science And Technology; South China Agricultural University
Priority date: 2022-07-26
Filing date: 2022-07-26
Publication date: 2023-04-14
Anticipated expiration: 2032-07-26

Abstract

The utility model discloses an insect action viewing device. The device is including observing the pipe, the one end of observing the pipe is equipped with the opening, the opening one end of observing the pipe is connected with the stopper, the size of stopper with the opening size of observing the pipe suits, be provided with the air vent on the stopper, be connected with the dwang on the stopper. The device can visually observe the insect behaviors in 360 degrees in an all-round way, is particularly suitable for observing the parasitic and spawning behaviors of parasitic wasps, and can be used for observing the parasitic and spawning behaviors of the parasitic wasps in the interaction process of different types of parasitic wasps and hosts.

Description

Insect behavior observation device

Technical Field

The utility model relates to an insect viewing device's technical field, more specifically relates to an insect action viewing device.

Background

Parasitic wasps, i.e., parasitic hymenoptera insects, are a class of important natural enemy insects, are of various species and complex habits, and play an extremely important role in biological pest control and comprehensive pest management. Usually parasitize in lepidoptera, coleoptera, hymenoptera, hemiptera and diptera, etc. insect larva, pupa and egg inside or outside, rely on the nutrient substance in the host body to guarantee the success eclosion of the young bee, finally achieve the purpose of self-propagation, also eliminated the host insect at the same time. Parasitic wasps can produce parasitic factors such as venom, multiple DNA viruses, abnormal cells, viroid and the like in the parasitic process. The parasitic factors can regulate the behavior, immune system, nutrition metabolism, endocrine, growth and development and other aspects of the host, and the strength and the type of the regulation and control function determine the application potential of the parasitic wasps in the biological control of pests.

The amethystodes lucida is a obligate dominant parasitic natural enemy on the diaphorina citri, has high host specialization, does not cause harm to introduced species, and can effectively control the population quantity of the diaphorina citri. Moreover, the control of the diaphorina citri is one of effective measures for controlling the spread of the citrus greening disease. In an interaction system of the amelogia lucida and the host diaphorina citri, eggs can be laid on the surface of the host, and parasitic factors such as venom and the like are injected into the host diaphorina citri during laying, so that the internal tissues of the host diaphorina citri can be seriously damaged, and the normal physiological function can not be exerted. In the biological experimental study, researchers can observe the process that the amelogia lucida parasitizes and spawns in the diaphorina citri, so that a host at one end is prevented from being parasitized for many times, and then other operations are carried out. Most of the existing observation devices adopt a culture dish to observe the parasitic wasp parasitizing host, the host and the parasitic wasp are put into the same culture dish, and then the host is removed under a microscope or after the parasitic behavior is observed by naked eyes. The parasitic host is transferred to another device. This observation is more suitable for larger host pests, such as lepidopteran larvae, although it also allows observation of the behavior of parasitic wasps to parasitize or lay eggs in or out of the host. The parasitizing behaviors of the ectoparasite and the endoparasite of the small host insects, such as hemiptera host insects, cannot observe the parasitizing and spawning behaviors of various types of parasitizing bees in an all-around 360-degree mode, and cannot accurately and intuitively observe whether the parasitizing bees spawn in the bodies or the body surfaces of the hosts (large and small).

Therefore, it is highly desirable to provide an observation device for 360 ° omni-directional observation of parasitic wasp parasitism and spawning behavior.

Disclosure of Invention

To the current technical problem of the aforesaid, an object of the utility model is to provide an insect action viewing device, this device can 360 all-round visual observation insect actions, is particularly useful for the parasitic wasp parasitic and the observation of the action of laying eggs, can be used to the parasitic wasp of different grade type (external parasitic and internal parasitic) and the observation of the parasitic wasp parasitic and the action of laying eggs of host (large-scale, small-size) interaction in-process.

The utility model discloses above-mentioned purpose is realized through following technical scheme:

the utility model provides an insect action viewing device, includes the observation pipe, the one end of observation pipe is equipped with the opening, the opening one end of observation pipe is connected with the stopper, the size of stopper with the opening size of observation pipe suits, be provided with the air vent on the stopper, be connected with the dwang on the stopper.

The utility model discloses mainly constitute by observation tube, stopper and dwang, can be used to observe the parasitism and the action of laying eggs of small-size host insect, if when the object is bright abdomen glaze wasp, bright abdomen glaze wasp parasitism action and the action of laying eggs are short-lived, can produce bull ovum in that one head host is external, and final this kind of parasitism action of crossing can lead to the host dead. Consequently, this device adopts the observation tube, can guarantee that parasitic wasp searches the host fast to observe parasitic and the action of laying eggs of parasitic wasp in the short time, through setting up the dwang, the sight that causes when avoiding hand and observation tube direct contact shelters from can be used to 360 all-round observations parasitic wasp parasitizes and the action of laying eggs, thereby guarantees effectively, directly perceived and save time 360 all-round observations to bright abdomen glaze wasp and host interaction action. The stopper is used for preventing parasitic bee and host escape, is provided with the air vent on the stopper simultaneously, can guarantee to observe inside and outside circulation of air of pipe, and the dwang can insert inside the stopper, with stopper zonulae occludens, can drive the stopper and observe the pipe and rotate together along with the rotation of dwang. When the device is used specifically, a user can operate the device with one hand to observe parasitic behavior of parasitic wasps for 360 degrees, and the other hand can be liberated to adjust the focal length of the microscope.

Preferably, the other end of the observation tube is a closed dome, and the observation tube is a circular tube. The observation tube is a circular tube, so that interaction behaviors of parasitic wasps and hosts can be observed conveniently at 360 degrees.

Preferably, the plug is an elastic plug, and the plug is in interference fit with the observation tube; for a better interference fit with the sight tube, the plug may preferably be a foam plug.

Preferably, the plug is a truncated cone or a cylinder.

Further preferably, the plug is a truncated cone. The diameter of the upper bottom of the plug of the cone is smaller than that of the lower bottom, so that the plug is more favorable for being plugged into (or taken out of) the observation tube.

Preferably, 4 to 6 vent holes are provided. The vent holes are arranged to ensure air circulation in the device to ensure survival of parasitic bees and hosts in the observation device.

Preferably, one end of the rotating rod close to the stopper is a tip, specifically, the tip may be a cone shape, and the rotating rod penetrates into the vent hole of the stopper through the tip and is connected with the stopper. The tip is arranged, so that the tip can be conveniently inserted into the vent hole and fixed with the plug; on the other hand, the egg picking device is convenient for picking eggs and can be used for picking out egg blocks of ectoparasite bees in the interaction experiment of the parasitic wasps and the hosts.

Preferably, one end of the rotating rod, which is far away from the plug, is connected with a guide brush. The guide brush can be used to guide parasitic bees or host pests out of the observation tube without causing damage thereto. More specifically, the guide brush adopts soft animal hair as a material, and avoids damaging the insect body when guiding the parasitic wasp or the host away from the observation tube.

Preferably, the rotating rod is provided with an anti-slip part. The antiskid part is convenient for drive the handheld operation when observing the pipe rotation.

Preferably, the anti-skid part is a spiral ring formed by winding a spiral wire, one end of the spiral wire, which is far away from the plug, is wound to form a ring opening, and the guide brush is arranged in the ring opening. The arrangement of the ring opening is convenient for manufacturing the guide brush, and the size of the guide brush can be changed according to the size of the ring opening.

Preferably, the sight tube, the stopper, the rotating lever and the guide brush are detachably connected to each other.

Preferably, the weight of the rotating rod does not usually exceed the weight of the observation tube and the plug, and the horizontal balance state of the observation tube can be ensured.

Compared with the prior art, the utility model discloses following beneficial effect has:

the utility model provides a modular viewing device with choose ovum and guiding device has solved the trouble of present 360 all-round observations of parasitic wasp parasitism and spawning behavior to parasitic wasp and host interaction in-process, 360 observations of solution of integration, choose the problem of ovum and injury-free guide, can be used for the observation of various types parasitic wasp and host interaction behavior for a long time, perhaps various types insect mating, get the observation and the research of behaviors such as food, parasitism, spawning. Device modern design, simple structure, preparation low cost, the operation is simple and easy, convenient and practical can be used to the observation of multiple insect action, and the device size can carry out selective adjustment according to the insect size.

Drawings

Fig. 1 is a schematic structural view of the device for observing insect behaviors of the present invention.

Fig. 2 is a schematic view of the structure of the stopper of fig. 1.

Fig. 3 is a schematic view showing the structure of the rotating rod and the guide brush in fig. 1.

In the figure, 1, a guide brush; 2. rotating the rod; 3. a plug; 4. an observation tube; 5. a vent hole; 6. an anti-slip portion; 7. and (4) looping.

Detailed Description

The following examples are given by way of illustration only and are not to be construed as limiting the invention. For a better understanding of the present embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent actual product dimensions. It will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the invention.

In describing embodiments of the present invention, if the terms "length," "width," "thickness," "height," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," etc., indicate that the orientation and positional relationship is based on that shown in the associated drawings, which is intended only for convenience of description and simplicity of description, and is not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, the above terms are not to be construed as limiting the present invention.

Example 1

As shown in figure 1, the insect behavior observation device comprises an observation tube 4, a plug 3 and a rotating rod 2, wherein one end of the observation tube 4 is a closed dome, and the other end of the observation tube is provided with an opening. Observe 4 open-ended one ends of pipe and be connected with stopper 3, the size of stopper 3 and the opening size of observing pipe 4 suit, still be provided with air vent 5 on the stopper 3, be connected with dwang 2 on the stopper 3.

In this embodiment, in order to facilitate observation of the parasitism and oviposition behavior of the bright belly glaze wasps on the host diaphorina citri, the observation tube 4 is a circular tube made of glass, such as a commonly used cylindrical finger-shaped tube with an original bottom, which is simple, convenient and practical, and has low cost. The glass material is convenient for insect behavior observation, and the cylindrical shape is convenient for 360-degree rolling observation under a microscope.

In this embodiment, the plug 3 is an elastic plug, and the plug 3 is in interference fit with the observation tube 4; the plug 3 may preferably be a foam plug for a better interference fit with the sight tube 4.

In this embodiment, the plug 3 is a truncated cone, and the truncated cone-shaped plug 3 is more favorable for screwing into the observation tube 4 (or taking out from the observation tube 4), so as to plug the opening of the observation tube 4, and the operation is convenient.

As shown in fig. 2, in this embodiment, 6 vent holes 5 are provided on the plug 3, which is convenient for observing the circulation of air in the tube 4 to the outside, and ensures the survival of amelogia lucida and diaphorina citri during the experiment.

In the present embodiment, an end of the rotating lever 2 near the stopper 3 is a pointed end, such as a conical shape, and more specifically, the rotating lever 2 may be embodied in a needle shape, such as a common acupuncture needle. The tip of the rotating rod 2 can penetrate into any position (such as a vent hole) of the plug 3, and then is connected with the plug 3 into a whole. The tip is arranged so as to be convenient to prick into the vent hole 5 and fixed with the plug 3; on the other hand, the method is convenient for picking eggs, and can be used for picking out egg blocks of ectoparasitic bees in the interaction experiment of the parasitic bees and hosts.

In this embodiment, the specific operation of the above device is: after putting into observation tube 4 parasitic wasp and host, guarantee the normal survival of parasitic wasp and host through air vent 5 on stopper 3, insert dwang 2 on the stopper 3 and fix together, the hand is held between the fingers one end of dwang 2 and is rotated, drives observation tube 4 and rotates, can reach 360 observation parasitic wasps and host between parasitism and the action of laying eggs.

Example 2

As shown in figure 1, the insect behavior observation device comprises an observation tube 4, a plug 3 and a rotating rod 2, wherein one end of the observation tube 4 is a closed dome, and the other end of the observation tube is provided with an opening. 4 open-ended one ends of observation pipe are connected with stopper 3, and the size of stopper 3 suits with the opening size of observation pipe 4, still is provided with air vent 5 on the stopper 3, is connected with dwang 2 on the stopper 3.

In this embodiment, in order to facilitate observation of the parasitism and spawning behavior of the belly-bright glaze wasps on the host diaphorina citri, the observation tube 4 is a circular tube made of glass, such as a commonly used cylindrical finger-shaped tube with an original bottom, which is simple, convenient and practical, and has low cost. The glass material is convenient for insect behavior observation, and the cylindrical shape is convenient for 360-degree rolling observation under a microscope.

In the present embodiment, the plug 3 is an elastic plug, and the plug 3 is in interference fit with the observation tube 4; the plug 3 may preferably be a foam plug for a better interference fit with the viewing tube 4.

In this embodiment, the plug 3 is a cylinder, and the plug 3 of the truncated cone is more beneficial to screwing into the observation tube 4 (or taking out from the observation tube 4 in a rotating manner), so that the opening of the observation tube 4 is conveniently plugged, and the operation is convenient.

As shown in fig. 2, in this embodiment, 4 vent holes 5 are provided on the plug 3, which is convenient for observing the circulation of air in the tube 4 to the outside, and ensures the survival of the ameliopsidae and the diaphorina citri during the experiment.

As shown in fig. 3, in the present embodiment, one end of the rotating lever 2 near the stopper 3 is a sharp end, such as a conical shape, and more specifically, a needle shape, such as a common silver needle, can be provided. The tip of the rotating rod 2 can penetrate into any position (such as a vent hole) of the plug 3, and then is connected with the plug 3 into a whole. The tip is arranged to facilitate the penetration into the vent hole 5 and the fixation with the plug 3; on the other hand, the method is convenient for picking eggs, and can be used for picking out egg blocks of ectoparasitic bees in the interaction experiment of the parasitic bees and hosts.

In the present embodiment, the anti-slip part 6 is provided on the rotating rod 2, the anti-slip part 6 is a spiral ring formed by a spiral wire, and one end of the spiral wire far away from the plug 3 is encircled to form a ring opening 7.

In this embodiment, the specific operation of the above device is: after putting into observation tube 4 with parasitic bee and host, guarantee the normal survival of parasitic bee and host through air vent 5 on stopper 3, insert dwang 2 on the stopper 3, be in the same place dwang 2 and stopper 3 are fixed, and the one end of holding the antiskid portion 6 on the dwang 2 with the hand rotates, drives observation tube 4 and rotates, can reach 360 observations parasitism and the behavior of laying eggs between parasitic bee and the host. When the parasitic wasp completes the parasitic or oviposition behavior, the rotating rod 2 is adopted to remove the wasp eggs outside the host body.

Example 3

In this embodiment, in order to facilitate observing the parasitism and spawning behavior of the belly glaze wasps on the host diaphorina citri, the observation tube 4 is a round tube made of glass, such as a commonly used cylindrical round-bottom glass cuvette, which is simple, convenient and practical, and has low cost. The glass material is convenient for insect behavior observation, and the cylindrical shape is convenient for 360-degree rolling observation under a microscope.

In this embodiment, the plug 3 is a truncated cone, and the plug 3 of the truncated cone is more beneficial to screwing into the observation tube 4 (or taking out from the observation tube 4), so as to plug the opening of the observation tube 4, and the operation is convenient.

In this embodiment, as shown in fig. 3, the end of the turning rod 2 near the stopper 3 is pointed, such as conical, more specifically, it can be needle-shaped, such as a common syringe needle. The tip of the rotating rod 2 can penetrate into any position of the plug 3, and then is connected with the plug 3 into a whole. The tip is arranged to facilitate the penetration into the vent hole 5 and the fixation with the plug 3; on the other hand, the egg picking device is convenient for picking eggs and can be used for picking out egg blocks of ectoparasite bees in the interaction experiment of the parasitic wasps and the hosts.

In this embodiment, a guiding brush 1 is connected to the end of the turning rod 2 remote from the stopper 3.

The guide brush 1 can be connected with the ring opening 7 on the rotating rod 2 through a double-sided adhesive tape.

In this embodiment, the above apparatus is implemented by the following steps: after putting into observation tube 4 with parasitic bee and host, guaranteeing the normal survival of parasitic bee and host through air vent 5 on the stopper 3, inserting dwang 2 on the stopper 3, being in the same place dwang 2 and stopper 3 are fixed, and the one end of the antiskid portion 6 on the dwang 2 is held between the fingers to the hand rotates, drives observation tube 4 and rotates, can reach 360 observations parasitic and the oviposition action between parasitic bee and the host. Dwang 2 and guide brush 1 unite into a whole through the annular opening 7, treat that parasitic wasp accomplishes parasitism or when laying eggs the action, draw parasitic wasp or host from observation tube 4 gently through guide brush 1 to adopt dwang 2 to choose the external bee ovum of host and remove.

It should be finally noted that the above embodiments are only intended to illustrate the technical solutions of the present invention, and not to limit the scope of the present invention, and that those skilled in the art can make variations or modifications in other different forms based on the above description and thought, and all embodiments need not be exhaustive. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. The utility model provides an insect action viewing device, its characterized in that, is including observing pipe (4), the one end of observing pipe (4) is equipped with the opening, the opening one end of observing pipe (4) is connected with stopper (3), the size of stopper (3) with the opening size of observing pipe (4) suits, be provided with air vent (5) on stopper (3), be connected with dwang (2) on stopper (3).

2. The insect behavior observing device according to claim 1, characterized in that the other end of the observing tube (4) is a closed dome, and the observing tube (4) is a circular tube.

3. An insect behaviour viewing device according to claim 1, wherein the plug (3) is an elastomeric plug, the plug (3) being an interference fit with the viewing tube (4).

4. Insect behaviour viewing device according to claim 1, characterised in that said plug (3) is a cone or a cylinder.

5. An insect behavior observing device according to claim 1, wherein 4 to 6 air vents (5) are provided.

6. The insect behavior observing device according to claim 1, wherein the rotating rod (2) has a pointed end at an end thereof close to the stopper (3), and the rotating rod (2) penetrates into the vent hole (5) of the stopper (3) through the pointed end and is connected to the stopper (3).

7. An insect behaviour viewing device according to claim 1 or 6, characterised in that a guiding brush (1) is attached to the end of the swivelling lever (2) remote from the plug (3).

8. An insect behavior observing device according to claim 7, wherein a non-slip portion (6) is provided on the rotating lever (2).

9. An insect behavior observing device according to claim 8, wherein the non-slip portion (6) is a spiral loop formed by a spiral wire, one end of the spiral wire far away from the plug (3) is surrounded to form a ring opening (7), and the guide brush (1) is arranged in the ring opening (7).

10. An insect behavior observing apparatus according to claim 7, wherein the observing tube (4), the stopper (3), the rotating lever (2) and the guide brush (1) are detachably connected to each other.