CN218778956U

CN218778956U - Instrument device for simulating myocardial cell volume overload

Info

Publication number: CN218778956U
Application number: CN202220081518.2U
Authority: CN
Inventors: 邹云增; 丁志文; 徐冉; 吴剑; 彭娟; 杨春杰
Original assignee: Zhongshan Hospital Fudan University
Current assignee: Zhongshan Hospital Fudan University
Priority date: 2022-01-11
Filing date: 2022-01-11
Publication date: 2023-03-31
Anticipated expiration: 2032-01-11

Abstract

The application discloses an apparatus device for simulating myocardial cell volume overload, which is characterized by comprising a culture box body and a cell culture dish for culturing myocardial cells, wherein the cell culture dish is a culture dish capable of being stretched leftwards and rightwards; the left side and the right side of the cell culture dish are both connected with a movable seat. The device that this application provided arranges the cardiomyocyte according to certain direction when the inoculation, can realize directional tensile this cell, and the guide post is periodic to be removed, realizes periodic tensile this cell, and to sum up two points, this device can simulate internal heart volume overload.

Description

Instrument device for simulating myocardial cell volume overload

Technical Field

The application relates to an apparatus for simulating myocardial cell volume overload, in particular to a volume overload model for preparing myocardial cells in vitro, belonging to the technical field of medical test instruments.

Background

Cardiac overload is a common and important cause of heart failure. Cardiac load is divided into two types, volume load and pressure load. Volume loading is the load that the myocardium is subjected to before contraction, which is equivalent to ventricular end-diastolic volume or pressure, also known as preload; pressure loading is the resistance encountered during myocardial contraction, i.e., the pressure to be overcome during ventricular ejection, also known as afterload. The cardiac signal paths affected by volume loading and pressure loading are different. In mouse models, aortic ligation and aortic valve regurgitation are commonly used to simulate cardiac pressure overload and volume overload. At the cellular level, myocardial pressure overload can be simulated using drugs such as angiotensin II, however volume overload has no in vitro model. In order to further clarify the internal mechanism of volume overload at the level of gene, molecule, cell, etc., it is necessary to establish a set of cell culture model for in vitro simulation of volume overload, so that through in vitro experiments, detailed molecular mechanisms of volume overload caused by various factors are found, a new method is provided for clinically treating heart failure caused by volume overload, and relevant theoretical theories are enriched.

SUMMERY OF THE UTILITY MODEL

The application aims to provide an in vitro model of cardiac volume overload, which provides a foundation for deeply explaining the intrinsic mechanism of cardiac volume overload from the gene, molecule and cell level.

In order to achieve the above object, the present application provides an apparatus device for simulating myocardial cell volume overload, comprising a culture box and a cell culture dish for culturing myocardial cells, wherein the cell culture dish is a culture dish capable of being stretched left and right; the cell culture dish is arranged at the left and the right both sides are connected with a movable seat.

Preferably, the moving distance of the moving seat should be not less than 10% of the length of the cell culture dish.

Preferably, the cell culture dish is rectangular and is made of silica gel.

Preferably, the cell culture dish is provided with a cell shape arrangement container which is used in a matching way, and the cell shape arrangement container is hung on the culture box body through handles positioned at four corners so that a gap exists between the cell culture dish and the cell shape arrangement container.

Preferably, be equipped with the screen cloth on the container is arranged to the cell shape, the screen cloth aperture is for length 18um, wide 2.5um.

Preferably, the screen is a metal screen.

Preferably, the movable seat is a sliding module arranged on the base of the culture box body, the sliding module is driven by a motor, and a guide column is arranged on the sliding module; rigid plastic is connected to the left and right sides of cell culture dish, is equipped with the round hole of cooperation guide post on the rigid plastic, and the cell culture dish is installed on the guide post of left and right sides slip module through the round hole of left and right sides.

The device that this application provided arranges the cardiomyocyte according to certain direction when the inoculation, can realize directional tensile this cell, and the guide post is periodic to be removed, realizes periodic tensile this cell, and to sum up two points, this device can simulate internal heart volume overload.

Drawings

FIG. 1 is a schematic view of an embodiment schematic diagram of the incubator;

FIG. 2 is a schematic view of a cell culture dish provided in the examples;

FIG. 3 is a schematic view of a cell shape arrangement container provided in the examples.

Detailed Description

In order to make the present application more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.

Examples

The embodiment provides an apparatus device for simulating capacity overload of myocardial cells, including incubator body 1 and cell culture dish 2 for culturing myocardial cells, cell culture dish 2 is rectangular, the material is the silica gel material, tensile attribute has, the removal seat is all connected to the 2 left and right sides of cell culture dish, the displacement distance that the seat was removed to the unilateral should be no less than 10% of 2 length of cell culture dish, the seat is removed to the both sides removes and then tensile cell culture dish 2 to the direction removal of keeping away from respectively, make the tensile range of cell culture dish 2 can reach more than 20% the most.

Optionally, the cell culture dish 2 is provided with a cell shape arrangement container 3 which is used in a matched manner, the cell shape arrangement container 3 is hung on the culture box body 1 through handles positioned at four corners so that a gap exists between the cell shape arrangement container 3 and the culture box body 1, the cell shape arrangement container 3 is in accordance with the cell culture dish 2 in size and can be just taken and placed in the culture box body 1, the cell shape arrangement container 3 is matched with the cell culture dish 2 for use and is used for placing a screen with irregular hole number, each hole of the screen is 18um in length and 2.5um in width, and therefore, the myocardial cells can be conveniently planted in the cell culture dish 2 according to a certain arrangement sequence; optionally, the screen is made of a metal material;

optionally, the connection structure who removes seat and cell culture dish 2 adopts the guide post, it sets up to installing the sliding module on 1 base of incubator body to remove the seat, sliding module's drive is by motor drive, the last guide post of installing of sliding module, rigid plastic is connected to the left and right sides of cell culture dish 2, set up the round hole of cooperation guide post on the rigid plastic, cell culture dish 2 is installed on the guide post of the left and right sides sliding module through the round hole of the left and right sides, the change of cell culture dish 2 of being convenient for.

The apparatus for simulating the volume overload of the cardiomyocytes provided by the embodiment simulates the volume overload of the heart in vivo by transversely stretching the cardiomyocytes; in particular, the method comprises the following steps of, placing the cell culture dish 2 on a guide column in the box body 1; the cell shape arrangement container 3 is placed on the cell culture dish 2, a culture medium is added, and after the cells naturally settle to the bottom of the cell culture dish 2 through the cell shape arrangement container 3, the container is taken out, and the cardiomyocytes are planted in the cell culture dish 2. After the cells adhere to the wall, the motor of the movable seat is controlled to drive the left (right) side guide column to periodically move. All cardiomyocytes in the culture dish were subjected to a lateral pulling force at this time, thereby achieving a simulated heart volume overload in vitro.

Claims

1. An apparatus device for simulating myocardial cell volume overload is characterized by comprising a culture box body and a cell culture dish for culturing myocardial cells, wherein the cell culture dish is a left-right stretchable culture dish which is made of silica gel and has stretching property; the left side and the right side of the cell culture dish are both connected with a movable seat; the cell culture dish is provided with a cell shape arrangement container which is used in a matched mode, and the cell shape arrangement container is hung on the culture box body through handles located at four corners, so that a gap exists between the cell culture dish and the cell shape arrangement container.

2. The apparatus for simulating myocardial cell volume overload of claim 1, wherein the moving seat has a moving distance not less than 10% of the length of the cell culture dish in the stretching direction.

3. The apparatus for simulating myocardial cell volume overload according to claim 1, wherein the cell culture dish is rectangular and is made of silica gel.

4. The apparatus for simulating myocardial cell volume overload of claim 1, wherein the cell shape arranging container has a screen with 18um long and 2.5um wide.

5. The apparatus for simulating myocardial cell volume overload according to claim 4, wherein the mesh is metal mesh.

6. The apparatus for simulating myocardial cell volume overload of claim 1, wherein the moving seat is one sliding module set on the base of the culture box and driven with motor and with guide column; rigid plastic is connected to the left and right sides of cell culture dish, is equipped with the round hole of cooperation guide post on the rigid plastic, and the cell culture dish is installed on the guide post of left and right sides slip module through the round hole of left and right sides.