CN201268703Y - Apparatus for sequentially observing influence of different electric field directions to cell biology behavior - Google Patents
Apparatus for sequentially observing influence of different electric field directions to cell biology behavior Download PDFInfo
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- CN201268703Y CN201268703Y CNU2008200999665U CN200820099966U CN201268703Y CN 201268703 Y CN201268703 Y CN 201268703Y CN U2008200999665 U CNU2008200999665 U CN U2008200999665U CN 200820099966 U CN200820099966 U CN 200820099966U CN 201268703 Y CN201268703 Y CN 201268703Y
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- patchhole
- culture medium
- electric field
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Abstract
The utility model relates to a device for sequentially observing influences of different electric field directions on cell biological behaviors, which comprises a cultivation observing component, wherein the cultivation observing component comprises four cultivation basal cells and an observing cell, the four cultivation basal cells are respectively arranged into a whole with the observing cell and are arranged on the periphery of the observing cell in a cross shape. Under the condition that the observing window is not moved, the utility model can replace the cultivation medium in the direction vertical to a DC electric field, and reduce the possible influence of the cultivation medium flowing on cell behaviors when the cultivation medium is replaced in the direction parallel to the DC electric field. Under the condition that the observation window is not moved, the utility model can also change the direction of the DC electric field, and sequentially observe the influence of two electric fields in vertical directions on the cell moving direction to enable the test result to be more objective and reliable.
Description
Technical field
The utility model relates to the cytobiology field, the device of the influence of the different direction of an electric field pair cell of particularly a kind of sequential property observation biological behaviour.
Background technology
DC electric field (direct-currentel ectric fields dcEF) extensively exists in organism, be since multiple charged ion (as Na
+, K
+, Ca
2+, HCO
3 -, C1
-Deng) inside and outside the cell, between cell and the cell, the difference that distributes of tissue and organ different sites produces.In recent years, people pay attention to gradually the Biological effects of DC electric field and go deep into, relate to cytobiology every field such as organizing formation, inflammatory reaction and injury repairing in the fetal development, confirmed the motion of various kinds of cell, the influence that division is subjected to DC electric field.The biological effect research of DC electric field mainly is on the cell levels biological behaviour of cell under the DC electric field to be studied at present.In some cases, research needs to investigate the influence of different direction of an electric field pair cell direction of motion, and the influence of the mobile pair cell biological behaviour of substratum when changing cell culture medium, but research device of the prior art can only be observed from a kind of biological behaviour of direction of an electric field pair cell, and can only change substratum from the direction parallel, can't satisfy the research demand with electric field.
The utility model content
In view of this, the purpose of this utility model is to provide the device of the influence of the different direction of an electric field pair cell of a kind of sequential property observation biological behaviour.
For achieving the above object, the device of the influence of the different direction of an electric field pair cell of sequential property observation of the present utility model biological behaviour, comprise cultivating and observe assembly, described cultivation is observed assembly and is comprised Culture Medium Laboratory and observation ward, described Culture Medium Laboratory is four, be set to one with observation ward respectively, four Culture Medium Laboratories are cross and are arranged on around the observation ward.
Further, described Culture Medium Laboratory height is at least 3 times of observation ward height;
Further, at least one is trapezoidal for described Culture Medium Laboratory, and described trapezoidal Culture Medium Laboratory and observation ward's connected component are the trapezoid upper base;
Further, also comprise fixation kit, described fixation kit comprises container and container cover, and at least two fixed orificess are set on the container cover, inserts column in the fixed orifices, and described column is used in level or/and fixing cultivation of vertical direction observed assembly;
Further, vision slit, electrode patchhole, salt bridge patchhole and substratum also are set on the described container cover and change the pipeline patchhole; The quantity of electrode patchhole, salt bridge patchhole and substratum replacing pipeline patchhole is corresponding with the quantity of Culture Medium Laboratory; The position of vision slit is corresponding with the position of observation ward, and the position of electrode patchhole is corresponding with the position of Culture Medium Laboratory and observation ward's connection place, and the salt bridge patchhole is corresponding with the position of Culture Medium Laboratory with the position that substratum is changed the pipeline patchhole;
Further, described Culture Medium Laboratory is a rectangle, described salt bridge patchhole is or/and the position of substratum replacing pipeline patchhole is corresponding with cultivation observation assembly diagonal lines two ends, described fixed orifices and electrode patchhole, salt bridge patchhole are or/and substratum is changed the pipeline patchhole overlaps, and described column is a hollow pillar stand.
The beneficial effects of the utility model are:
(1) adopt ready-formed, independently, the cultivation that is set to one observes assembly, do not need steps such as interim gluing or stickup during experiment, can reach to simplify the operation, the shortening time, avoid polluting the purpose of assembly and substratum; Can clean according to a conventional method, sterilize before using, the sterilization back is reusable, reduces the error that is caused because of each interim producing device; Directly cell climbing sheet is inserted observation ward during use and get final product, easy to use;
(2) adopt four Culture Medium Laboratories to be the cross observation ward's structure all around that is arranged on, further expanded the function of research device, can be under the situation of mobile viewing window not, realization is from changing substratum with the perpendicular direction of DC electric field, reduces the influence that the mobile pair cell behavior of substratum may cause when changing substratum with the DC electric field parallel direction; Also can under the situation of mobile viewing window not, change the DC electric field direction, the influence of vertical two the electric field pair cell direction of motion of sequential property direction of observation, thus make experimental result more objective, reliable;
(3) adopt the Culture Medium Laboratory height to be at least 3 times structure of observation ward's height, can well be kept at substratum in the Culture Medium Laboratory, can not make the substratum of positive and negative electrode cover the observation ward top when adding the more or mobile culture dish of substratum, extra current occurring passes through, the cleaning that can also keep the observation ward top in addition guarantees good observing effect;
(4) at least one is trapezoidal to adopt Culture Medium Laboratory, and trapezoidal Culture Medium Laboratory and observation ward's connected component are the structure of trapezoid upper base, can play guide effect, can easily and quickly cell climbing sheet be inserted observation ward;
(5) adopt the fixation kit that constitutes by container and container cover, at least two fixed orificess are set on the container cover, insert the structure of column in the fixed orifices, when in container, putting into cultivation observation assembly and covered container lid, can block the Culture Medium Laboratory inboard by column, reach to fix to cultivate and observe assembly and make the stable purpose of whole apparatus structure, assurance cultivation observation assembly can not be moved or rock in container and viewing window is moved, and is convenient to observational study;
(6) microscopic examination needs light to see through in the middle of install, and adopts the structure that vision slit is set on the container cover, can reduce the stopping of light, guarantees good observing effect; Adopt the structure that the electrode patchhole is set on the container cover, be used for fixing electrode, in real time the voltage change of both sides, monitoring observation chamber; Substratum is through may keeping the vigor of cell owing to cellular metabolism after long-time, should change substratum this moment makes cell obtain suitable environment, maintain vigour, so that observe for a long time, adopt the structure that substratum is changed the pipeline patchhole is set on the container cover, can under the situation of mobile viewing window not, change substratum easily;
(7) adopt the salt bridge patchhole or/and the position of substratum replacing pipeline patchhole is corresponding with cultivation observation assembly diagonal lines two ends, fixed orifices and electrode patchhole, salt bridge patchhole are or/and substratum is changed the pipeline patchhole overlaps, in fixed orifices, insert the structure of hollow pillar stand, not only be simplified to fix the making step of assembly, make its compact construction, and further expanded electrode patchhole, salt bridge patchhole or/and substratum is changed the function of pipeline patchhole, make it have dual function.
Description of drawings
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing the utility model is described in further detail, wherein:
Fig. 1 is that the sectional view under assembly and the fixation kit assembled state is observed in cultivation of the present utility model;
Fig. 2 is the structural representation of container cover of the present utility model.
Embodiment
Hereinafter with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail.
In the present embodiment, the device of the influence of the different direction of an electric field pair cell of sequential property observation biological behaviour comprises cultivation observation assembly and fixation kit.
Fig. 1 is that the sectional view under assembly and the fixation kit assembled state is observed in cultivation of the present utility model, Fig. 2 is the structural representation of container cover of the present utility model, as shown in the figure: cultivate the observation assembly and comprise observation ward 1 and four Culture Medium Laboratories: Culture Medium Laboratory I21, Culture Medium Laboratory II22, Culture Medium Laboratory III23 and Culture Medium Laboratory IV24, four Culture Medium Laboratories are set to one with observation ward 1 respectively, being cross is arranged on around the observation ward 1, the Culture Medium Laboratory height is 3 times of observation ward's 3 height, and Culture Medium Laboratory and observation ward 1 are connected; Culture Medium Laboratory at least one can be designed to trapezoidally, and trapezoidal Culture Medium Laboratory and observation ward's 1 connected component are the trapezoid upper base; Fixation kit comprises container 3 and container cover, a vision slit 41, four electrode patchholes 44, four salt bridge patchholes 42 and four substratum is set on the container cover changes pipeline patchholes 43; When in container, putting into cultivation observation assembly and covered container lid, the position of vision slit 41 is corresponding with the position of observation ward 1, the position of electrode patchhole 44 is corresponding with the position of connection place of Culture Medium Laboratory and observation ward 1, the position of salt bridge patchhole 42 is corresponding with the position of Culture Medium Laboratory, and the position of substratum replacing pipeline patchhole 43 is corresponding with the position of cultivating observation assembly diagonal lines two ends; Container cover top electrode patchhole 44 and substratum are changed pipeline patchhole 43 places and are inserted and fixedly install hollow pillar stand 45, the length of hollow pillar stand 45 is for can insert the Culture Medium Laboratory inboard, and assurance cultivation observation assembly can not be moved or rock in container and viewing window is moved.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (6)
1, the device of the influence of the different direction of an electric field pair cell of sequential property observation biological behaviour, comprise cultivating and observe assembly, it is characterized in that: described cultivation is observed assembly and is comprised Culture Medium Laboratory and observation ward (1), described Culture Medium Laboratory is four, be set to one with observation ward (1) respectively, four Culture Medium Laboratories are cross and are arranged on observation ward (1) all around.
2, the device of the influence of the different direction of an electric field pair cell of sequential property observation according to claim 1 biological behaviour, it is characterized in that: described Culture Medium Laboratory height is at least 3 times of observation ward (1) height.
3, the device of the influence of the different direction of an electric field pair cell of sequential property observation according to claim 2 biological behaviour, it is characterized in that: at least one is trapezoidal for described Culture Medium Laboratory, and described trapezoidal Culture Medium Laboratory and observation ward (1) connected component are the trapezoid upper base.
4, the device of the influence of the different direction of an electric field pair cell of sequential property observation according to claim 3 biological behaviour, it is characterized in that: also comprise fixation kit, described fixation kit comprises container (3) and container cover, at least two fixed orificess are set on the container cover, insert column in the fixed orifices, described column is used in level or/and fixing cultivation of vertical direction observed assembly.
5, the device of the influence of the different direction of an electric field pair cell of sequential property observation according to claim 4 biological behaviour is characterized in that: vision slit (41), electrode patchhole (44), salt bridge patchhole (42) and substratum also are set on the described container cover change pipeline patchhole (43); The quantity of electrode patchhole (44), salt bridge patchhole (42) and substratum replacing pipeline patchhole (43) is corresponding with the quantity of Culture Medium Laboratory; The position of vision slit (41) is corresponding with the position of observation ward (1), the position of electrode patchhole (44) is corresponding with the position of Culture Medium Laboratory and observation ward (1) connection place, and salt bridge patchhole (42) is corresponding with the position of Culture Medium Laboratory with the position that substratum is changed pipeline patchhole (43).
6, the device of the influence of the different direction of an electric field pair cell of sequential property observation according to claim 5 biological behaviour, it is characterized in that: described Culture Medium Laboratory is a rectangle, described salt bridge patchhole (42) is or/and the position of substratum replacing pipeline patchhole (43) is corresponding with cultivation observation assembly diagonal lines two ends, described fixed orifices and electrode patchhole (44), salt bridge patchhole (42) are or/and substratum is changed pipeline patchhole (43) overlaps, and described column is a hollow pillar stand.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200999665U CN201268703Y (en) | 2008-09-11 | 2008-09-11 | Apparatus for sequentially observing influence of different electric field directions to cell biology behavior |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200999665U CN201268703Y (en) | 2008-09-11 | 2008-09-11 | Apparatus for sequentially observing influence of different electric field directions to cell biology behavior |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201268703Y true CN201268703Y (en) | 2009-07-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200999665U Expired - Fee Related CN201268703Y (en) | 2008-09-11 | 2008-09-11 | Apparatus for sequentially observing influence of different electric field directions to cell biology behavior |
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|---|---|
| CN (1) | CN201268703Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787592A (en) * | 2010-03-17 | 2010-07-28 | 山东天鹅棉业机械股份有限公司 | Barbed nail roller assembly |
| CN103267660A (en) * | 2013-04-19 | 2013-08-28 | 山东大学 | Pathological processing humidity box for cell slide |
| CN109827898A (en) * | 2019-03-29 | 2019-05-31 | 河海大学 | A kind of metal corrosion test device |
-
2008
- 2008-09-11 CN CNU2008200999665U patent/CN201268703Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787592A (en) * | 2010-03-17 | 2010-07-28 | 山东天鹅棉业机械股份有限公司 | Barbed nail roller assembly |
| CN103267660A (en) * | 2013-04-19 | 2013-08-28 | 山东大学 | Pathological processing humidity box for cell slide |
| CN109827898A (en) * | 2019-03-29 | 2019-05-31 | 河海大学 | A kind of metal corrosion test device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090708 Termination date: 20100911 |