CN201296757Y - Improved device used for observing cell biological behaviors under direct current field - Google Patents

Improved device used for observing cell biological behaviors under direct current field Download PDF

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Publication number
CN201296757Y
CN201296757Y CN 200820099965 CN200820099965U CN201296757Y CN 201296757 Y CN201296757 Y CN 201296757Y CN 200820099965 CN200820099965 CN 200820099965 CN 200820099965 U CN200820099965 U CN 200820099965U CN 201296757 Y CN201296757 Y CN 201296757Y
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China
Prior art keywords
patchhole
culture medium
observation ward
improved device
medium laboratory
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Expired - Fee Related
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CN 200820099965
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Chinese (zh)
Inventor
李飞
冯华
卢佳友
李梅
朱刚
李丽
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Third Military Medical University TMMU
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Third Military Medical University TMMU
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses an improved device used for observing cell biological behaviors under direct current field, comprising culturing and observing components and fixing components; the culturing and observing components comprises an observing room and at least two culture medium rooms which are respectively integrated with the observing room into one body; the fixing component comprises a container and a container cover; the container is provided with at least two fixing holes which are internally inserted with posts; and the post are used for fixing the culturing and observing components in the horizontal or/and vertical direction. The improved device has simple and convenient manufacture and stable structure, can be used repeatedly, has good repetitiveness, can save experimental time and can be used for observing the influence of different directions of the electric field to the cell biological behaviors in sequence.

Description

The improved device that is used for observing cell biology behavior under the DC electric field
Technical field
The utility model relates to the cytobiology field, particularly a kind of improved device that is used for observing cell biology behavior under the DC electric field.
Background technology
DC electric field (direct-current electric fields dcEF) extensively exists in organism, be since multiple charged ion (as Na +, K +, Ca 2+, HCO 3 -, Cl -Deng) inside and outside the cell, between cell and the cell, the difference that distributes of tissue and organ different sites produces.In recent years, people pay attention to gradually the Biological effects of DC electric field and go deep into, relate to cytobiology every field such as organizing formation, inflammatory reaction and injury repairing in the fetal development, confirmed the motion of various kinds of cell, the influence that division is subjected to DC electric field.The biological effect research of DC electric field mainly is on the cell levels biological behaviour of cell under the DC electric field to be studied at present.In the prior art, the device that is used for this research comprises power supply, electrode exchange bottle, salt bridge, electrode, cell climbing sheet and cultivation observation assembly, cultivate the observation assembly assembles when each experiment temporarily, be about to cell climbing sheet and put into the observation groove, observing groove top stickup slide again, make observation ward, then observation ward is affixed to the base plate center of a square cell, make to cultivate and observe assembly.There is following defective in this device: (1) makes complicated, consuming time and can not reuse; (2) may there be error between each device of making and influences the accuracy of experimental result; Need the interim slide of pasting at every turn when (3) making, easily pollute cultivating observation assembly and substratum; (4) height of the cover glass pasted of observation ward top is difficult to stop that substratum covers, and substratum easily covers cover glass and influences observation when adding the too much or mobile culture dish of substratum; (4) can not be sequential the influence of the different direction of an electric field pair cells of property observation.
The utility model content
In view of this, the purpose of this utility model is to provide a kind of improved device that is used for observing cell biology behavior under the DC electric field, simple for production, Stability Analysis of Structures, reusable and good reproducibility, save experimental period, can be used for the influence of the different direction of an electric field pair cell of sequential property observation biological behaviour.
For achieving the above object, the improved device that is used for observing cell biology behavior under the DC electric field of the present utility model, comprise cultivating and observe assembly and fixation kit, described cultivation is observed assembly and is comprised observation ward and Culture Medium Laboratory, described Culture Medium Laboratory is at least two, fixedly installs with observation ward respectively to be one;
Described fixation kit comprises container and container cover, and at least two fixed orificess are set on the container cover, inserts column in the described fixed orifices, and described column is used in level or/and fixing cultivation of vertical direction observed assembly.
Further, vision slit, electrode patchhole, salt bridge patchhole and substratum also are set on the described container cover and change the pipeline patchhole; The quantity of electrode patchhole, salt bridge patchhole and substratum replacing pipeline patchhole is corresponding with the quantity of Culture Medium Laboratory; The position of vision slit is corresponding with the position of observation ward, and the position of electrode patchhole is corresponding with the position of Culture Medium Laboratory and observation ward's connection place, and the salt bridge patchhole is corresponding with the position of Culture Medium Laboratory with the position that substratum is changed the pipeline patchhole;
Further, described Culture Medium Laboratory is a rectangle, described fixed orifices and electrode patchhole, salt bridge patchhole are or/and substratum is changed the pipeline patchhole overlaps, described salt bridge patchhole is or/and the position of substratum replacing pipeline patchhole is corresponding with cultivation observation assembly diagonal lines two ends, and described column is a hollow pillar stand;
Further, described cultivation is observed assembly and is comprised Culture Medium Laboratory and observation ward, and Culture Medium Laboratory is four, is set to one with observation ward respectively, and four Culture Medium Laboratories are cross and are arranged on around the observation ward;
Further, described Culture Medium Laboratory height is at least 3 times of observation ward height;
Further, at least one is trapezoidal for described Culture Medium Laboratory, and described trapezoidal Culture Medium Laboratory and observation ward's connected component are the trapezoid upper base.
The beneficial effects of the utility model are:
(1) adopt ready-formed, independently, the cultivation that is set to one observes assembly, do not need steps such as interim stickup during experiment, can reach to simplify the operation, the shortening time, avoid polluting the purpose of assembly and substratum; Can clean according to a conventional method, sterilize before using, the sterilization back is reusable, reduces the error that is caused because of each interim producing device; Directly cell climbing sheet is inserted observation ward during use and get final product, easy to use;
(2) adopt four Culture Medium Laboratories to be the cross observation ward's structure all around that is arranged on, further expanded the function of research device, can be under the situation of mobile viewing window not, realization is from changing substratum with the perpendicular direction of DC electric field, reduces the influence that the mobile pair cell behavior of substratum may cause when changing substratum with the DC electric field parallel direction; Also can under the situation of mobile viewing window not, change the DC electric field direction, the influence of vertical two the electric field pair cell direction of motion of sequential property direction of observation, thus make experimental result more objective, reliable;
(3) adopt the Culture Medium Laboratory height to be at least 3 times structure of observation ward's height, can well be kept at substratum in the Culture Medium Laboratory, can not make the substratum of positive and negative electrode cover the observation ward top when adding the more or mobile culture dish of substratum, extra current occurring passes through, the cleaning that can also keep the observation ward top in addition guarantees good observing effect;
(4) at least one is trapezoidal to adopt Culture Medium Laboratory, and trapezoidal Culture Medium Laboratory and observation ward's connected component are the structure of trapezoid upper base, can play guide effect, can easily and quickly cell climbing sheet be inserted observation ward;
(5) adopt the fixation kit that constitutes by container and container cover, at least two fixed orificess are set on the container cover, insert the structure of column in the fixed orifices, when in container, putting into cultivation observation assembly and covered container lid, can block the Culture Medium Laboratory inboard by column, reach to fix to cultivate and observe assembly and make the stable purpose of whole apparatus structure, assurance cultivation observation assembly can not be moved or rock in container and viewing window is moved, and is convenient to observational study;
(6) microscopic examination needs light to see through in the middle of install, and adopts the structure that vision slit is set on the container cover, can reduce the stopping of light, guarantees good observing effect; Adopt the structure that the electrode patchhole is set on the container cover, be used for fixing electrode, in real time the voltage change of both sides, monitoring observation chamber; Substratum is through may keeping the vigor of cell owing to cellular metabolism after long-time, should change substratum this moment makes cell obtain suitable environment, maintain vigour, so that observe for a long time, adopt the structure that substratum is changed the pipeline patchhole is set on the container cover, can under the situation of mobile viewing window not, change substratum easily;
(7) adopt the salt bridge patchhole or/and the position of substratum replacing pipeline patchhole is corresponding with cultivation observation assembly diagonal lines two ends, fixed orifices and electrode patchhole, salt bridge patchhole are or/and substratum is changed the pipeline patchhole overlaps, in fixed orifices, insert the structure of hollow pillar stand, not only be simplified to fix the making step of assembly, make its compact construction, and further expanded electrode patchhole, salt bridge patchhole or/and substratum is changed the function of pipeline patchhole, make it have dual function.
Description of drawings
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing the utility model is described in further detail, wherein:
Fig. 1 is that the sectional view under assembly and the fixation kit assembled state is observed in cultivation of the present utility model;
Fig. 2 is the structural representation of container cover of the present utility model.
Embodiment
Hereinafter with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail.
Fig. 1 is that the sectional view under assembly and the fixation kit assembled state is observed in cultivation of the present utility model, Fig. 2 is the structural representation of container cover of the present utility model, as shown in the figure: cultivate the observation assembly and comprise observation ward 1 and four Culture Medium Laboratories: Culture Medium Laboratory I21, Culture Medium Laboratory II22, Culture Medium Laboratory III23 and Culture Medium Laboratory IV24, four Culture Medium Laboratories are set to one with observation ward 1 respectively, being cross is arranged on around the observation ward 1, the Culture Medium Laboratory height is 3 times of observation ward's 3 height, and Culture Medium Laboratory and observation ward 1 are connected; Culture Medium Laboratory at least one can be designed to trapezoidally, and trapezoidal Culture Medium Laboratory and observation ward's 1 connected component are the trapezoid upper base; Fixation kit comprises container 3 and container cover, a vision slit 41, four electrode patchholes 44, four salt bridge patchholes 42 and four substratum is set on the container cover changes pipeline patchholes 43; When in container, putting into cultivation observation assembly and covered container lid, the position of vision slit 41 is corresponding with the position of observation ward 1, the position of electrode patchhole 44 is corresponding with the position of connection place of Culture Medium Laboratory and observation ward 1, the position of salt bridge patchhole 42 is corresponding with the position of Culture Medium Laboratory, and the position of substratum replacing pipeline patchhole 43 is corresponding with the position of cultivating observation assembly diagonal lines two ends; Container cover top electrode patchhole 44 and substratum are changed pipeline patchhole 43 places and are inserted and fixedly install hollow pillar stand 45, the length of hollow pillar stand 45 is for can insert the Culture Medium Laboratory inboard, and assurance cultivation observation assembly can not be moved or rock in container and viewing window is moved.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.

Claims (6)

1, the improved device that is used for observing cell biology behavior under the DC electric field, comprise cultivating and observe assembly and fixation kit, it is characterized in that: described cultivation is observed assembly and is comprised observation ward (1) and Culture Medium Laboratory, described Culture Medium Laboratory is at least two, fixedly installs with observation ward (1) respectively to be one;
Described fixation kit comprises container (3) and container cover, and at least two fixed orificess are set on the container cover, inserts column in the described fixed orifices, and described column is used in level or/and fixing cultivation of vertical direction observed assembly.
2, the improved device that is used for observing cell biology behavior under the DC electric field according to claim 1 is characterized in that: vision slit (41), electrode patchhole (44), salt bridge patchhole (42) and substratum also are set on the described container cover change pipeline patchhole (43); The quantity of electrode patchhole (44), salt bridge patchhole (42) and substratum replacing pipeline patchhole (43) is corresponding with the quantity of Culture Medium Laboratory; The position of vision slit (41) is corresponding with the position of observation ward (1), the position of electrode patchhole (44) is corresponding with the position of Culture Medium Laboratory and observation ward (1) connection place, and salt bridge patchhole (42) is corresponding with the position of Culture Medium Laboratory with the position that substratum is changed pipeline patchhole (43).
3, the improved device that is used for observing cell biology behavior under the DC electric field according to claim 2, it is characterized in that: described Culture Medium Laboratory is a rectangle, described fixed orifices and electrode patchhole (44), salt bridge patchhole (42) are or/and substratum is changed pipeline patchhole (43) overlaps, described salt bridge patchhole (42) is or/and the position of substratum replacing pipeline patchhole (43) is corresponding with cultivation observation assembly diagonal lines two ends, and described column is a hollow pillar stand.
4, the improved device that is used for observing cell biology behavior under the DC electric field according to claim 3, it is characterized in that: described cultivation is observed assembly and is comprised Culture Medium Laboratory and observation ward (1), Culture Medium Laboratory is four, be set to one with observation ward (1) respectively, four Culture Medium Laboratories are cross and are arranged on observation ward (1) all around.
5, the improved device that is used for observing cell biology behavior under the DC electric field according to claim 4 is characterized in that: described Culture Medium Laboratory height is at least 3 times of observation ward (1) height.
6, the improved device that is used for observing cell biology behavior under the DC electric field according to claim 5 is characterized in that: at least one is trapezoidal for described Culture Medium Laboratory, and described trapezoidal Culture Medium Laboratory and observation ward (1) connected component are the trapezoid upper base.
CN 200820099965 2008-09-11 2008-09-11 Improved device used for observing cell biological behaviors under direct current field Expired - Fee Related CN201296757Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200820099965 CN201296757Y (en) 2008-09-11 2008-09-11 Improved device used for observing cell biological behaviors under direct current field

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200820099965 CN201296757Y (en) 2008-09-11 2008-09-11 Improved device used for observing cell biological behaviors under direct current field

Publications (1)

Publication Number Publication Date
CN201296757Y true CN201296757Y (en) 2009-08-26

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C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090826

Termination date: 20100911