CN210458217U - Micro-negative pressure cell culture device - Google Patents
Micro-negative pressure cell culture device Download PDFInfo
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- CN210458217U CN210458217U CN201920949088.XU CN201920949088U CN210458217U CN 210458217 U CN210458217 U CN 210458217U CN 201920949088 U CN201920949088 U CN 201920949088U CN 210458217 U CN210458217 U CN 210458217U
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- negative pressure
- cell culture
- culture device
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- pressure
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- 238000004113 cell culture Methods 0.000 title claims abstract description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 238000002347 injection Methods 0.000 claims abstract description 5
- 239000007924 injection Substances 0.000 claims abstract description 5
- 230000001105 regulatory effect Effects 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 7
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 19
- 210000002950 fibroblast Anatomy 0.000 abstract description 7
- 230000009193 crawling Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 210000001339 epidermal cell Anatomy 0.000 abstract description 4
- 206010020718 hyperplasia Diseases 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 15
- 208000027418 Wounds and injury Diseases 0.000 description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 7
- 210000002510 keratinocyte Anatomy 0.000 description 5
- 230000037311 normal skin Effects 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
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- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000019305 fibroblast migration Effects 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
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- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
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Abstract
The utility model provides a micro-negative pressure cell culture device, which comprises CO2Cell culture chamber, placed in the CO2A cell incubator, a pressure trimmer and a negative pressure pump in the cell incubator. The pressure fine adjuster comprises a pressure adjusting cavity and a water injection pipeline communicated with the side end, a water outlet pipeline is arranged at the bottom end of the pressure adjusting cavity, and a pipe joint is arranged at the top end of the pressure adjusting cavity; the cell culture device is provided with two tubes, one tube is connected with the negative pressure pump, and the other tube is arranged on the tube connector. Proved through experimental study, the utility model discloses well little negative pressure cell culture device has certain promotion effect to the cell crawl with the hyperplasia, and the epidermal cell and the fibroblast of external culture all show stronger crawling ability, provide useful exploration for clinical surface of a wound is restoreed.
Description
Technical Field
The utility model belongs to the technical field of experimental apparatus, concretely relates to cell (epidermis, fibroblast, vascular endothelial cell etc.) culture apparatus that can provide little negative pressure.
Background
The negative pressure closed drainage technology changes an open wound surface into a closed wound surface, can remove seepage of the wound surface at regular time under the action of rated negative pressure, can relieve edema among tissues, improve tissue microcirculation, promote capillary vessel regeneration, and reduce bacterial reproduction, thereby promoting the growth of granulation tissues and creating favorable conditions for covering the wound surface. The method is widely applied to the wound repair of the department of burn, surgery and orthopaedics.
The negative pressure value of the wound surface negative pressure drainage technology in application at present is-80 to-120 mmHg, the wound surface seepage can be effectively managed under the action of the negative pressure value, but the wound surface seepage has no obvious effect on the growth and creeping of epidermis, so in clinical practice, when fresh granulation grows on the base of the wound surface, a skin grafting operation can be selected to cover the wound surface or the wound surface is repaired by the normal epidermis around the wound surface to creep.
In clinical practice, after the wound surface has new granulation growth, if a certain micro negative pressure is given, the wound surface of a patient is better in healing speed and degree than the wound surface which is self-healed. However, the minimum negative pressure that can be generated by the negative pressure pump used in the prior art is generally-30 to-50 mmHg, and strictly speaking, the minimum negative pressure is not in the category of micro negative pressure, so that even if the negative pressure pump is adjusted to be the minimum, the minimum negative pressure still has little influence on the creeping of epidermal cells. Under the limitation of the probe technology of the negative pressure pump, the negative pressure value of the existing machine is difficult to further reduce to reach the micro negative pressure value (-5 to-10 mmHg) by negative pressure pump manufacturers at present. Therefore, whether the micro negative pressure can promote the wound healing or not can not be verified by adopting the current negative pressure closed drainage equipment.
SUMMERY OF THE UTILITY MODEL
The utility model provides a micro-negative pressure cell culture device which can provide micro-negative pressure environment for cell culture. In order to realize the purpose, the utility model discloses the technical scheme who adopts as follows:
the utility model provides a little negative pressure cell culture device has following technical characteristic: comprising CO2A cell culture chamber placed in the CO2A cell incubator, a pressure trimmer and a negative pressure pump in the cell incubator. The pressure fine adjuster comprises a pressure adjusting cavity and a water injection pipeline communicated with the side end, a water outlet pipeline is arranged at the bottom end of the pressure adjusting cavity, and a pipe joint is arranged at the top end of the pressure adjusting cavity; the cell culture device is provided with two tubes, one tube is connected with the negative pressure pump, and the other tube is arranged on the tube connector.
The utility model discloses in, place the negative pressure pump at CO2The reasons for the cell culture chamber, rather than being placed outside, are: CO to be absorbed by negative pressure pump2The gas is released in the external environment, the pump is placed outside, the carbon dioxide gas in the incubator can be consumed, and carbon dioxide needs to be added continuously; is placed in a cell culture box and can relatively maintain CO in the culture box2And (4) balancing the gas.
Preferably, in the micro-negative pressure cell culture device provided by the utility model, the device further comprises a pressure fine-adjustment device placing frame, which comprises an upper layer and a lower layer, wherein the upper layer is provided with a placing groove with a through hole on the bottom wall, and the lower layer is provided with a push-pull water receiving groove.
The pressure trimmer placing rack is also placed in the CO2In the cell culture box, the pressure trimmer is placed in the placing groove, two water outlet pipelines of the pressure trimmer penetrate through the through hole and are positioned above the push-pull water receiving groove, so that pressure adjustment is realized at any time, and when water in the water receiving groove is accumulated to a certain degree, the water receiving groove is taken out and poured.
Preferably, in the micro-negative pressure cell culture device provided by the utility model, the pressure fine-tuning ware is placed and is provided with two guide vanes on the inner wall of frame lower floor, and the both ends of plug-type water receiving tank are provided with the border of taking on the guide vane, and the width of water receiving tank is the same with the interval between two guide vanes. The structure of plug-type water receiving tank is more common among the prior art, the utility model discloses only enumerate one of them simplest mode, realize taking out of water receiving tank and water receiving after the installation.
Preferably, in the utility model provides a micro-negative pressure cell culture device, the cell culture ware includes circular chassis, sets up double-deck culture frame on circular chassis, sealed transparent sealing lid of detaining on circular chassis, sets up the hasp that goes on locking transparent sealing lid in circular chassis peripheral part, is provided with two pipes of being connected with negative pressure pump and pressure trimmer respectively on the circular chassis.
Preferably, in the micro-negative pressure cell culture device provided by the utility model, a valve is arranged on the water outlet pipeline and is positioned above the push-pull water receiving tank.
Preferably, in the micro-negative pressure cell culture device provided by the utility model, the front wall of the pressure regulating cavity is provided with scale marks.
Preferably, in the utility model provides an among the micro negative pressure cell culture device, the negative pressure pump has the display screen, and the negative pressure pump that the inventor chose for use when actually carrying out the experiment is CGBIO company's curasys negative pressure pump.
Action and effect of the utility model
According to the utility model provides a little negative pressure cell culture device owing to set up the pressure trimmer, through the liquid level height in adjusting the pressure regulating chamber, has realized forming-5-/-10 mmHg little negative pressure environment in the cell culture ware, and it is verified through the experiment, and the speed of crawling and the proliferation of epidermal cell under this little negative pressure environment is obviously faster than the experimental result under the non-little negative pressure environment, is favorable to simulating the exploration clinically, promotes the healing of the surface of a wound.
Furthermore, the utility model provides a pressure fine setting ware simple structure, the preparation cost is low, is favorable to the popularization and application in laboratory and clinic.
Drawings
FIG. 1 is a schematic structural diagram of a micro-negative pressure cell culture device according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a pressure trimmer according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a placement frame for a pressure trimmer according to an embodiment of the present invention;
FIG. 4 shows the microscopic results of the scratch test of normal skin keratinocytes in the embodiment of the present invention;
fig. 5 shows the microscopic result of the fibroblast scratch test in the embodiment of the present invention.
Detailed Description
The present invention will be described in detail with reference to the following embodiments and drawings. The following examples should not be construed, however, as limiting the scope of the invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.
FIG. 1 is a schematic structural diagram of a micro-negative pressure cell culture device according to an embodiment of the present invention.
As shown in FIG. 1, the micro-negative pressure cell culture apparatus 100 includes CO2A cell culture chamber 1 placed in the CO2A cell incubator 2, a pressure trimmer 3, a pressure trimmer placing frame 4 and a negative pressure pump 5 in the cell incubator.
CO2The cell culture box 1 is a common culture box for a biological laboratory, and the cell culture box 2 is a relatively sealed cell culture box and comprises a circular bottom frame, a double-layer culture frame 21 arranged on the circular bottom frame, a transparent sealing cover 22 sealed and buckled on the circular bottom frame, and a lock 23 arranged on the peripheral part of the circular bottom frame and used for locking the transparent sealing cover. The circular chassis is provided with two pipes 24 and 25 connected to the negative pressure pump 5 and the pressure trimmer 3, respectively.
Fig. 2 is a schematic structural diagram of a pressure trimmer according to an embodiment of the present invention.
As shown in fig. 1 and 2, the pressure trimmer 3 includes a pressure adjusting chamber 31, a water injection pipe 32 disposed at a side end of the pressure adjusting chamber 31, a water outlet pipe 33 disposed at a bottom end of the pressure adjusting chamber, and a pipe joint 34 disposed at a top end of the pressure adjusting chamber.
The front end of the pressure regulating cavity 31 is provided with scale marks for displaying water level, and the water injection pipeline 32 is used for injecting water into the pressure regulating cavity. The formation of a micro-negative pressure environment in the cell culture device 2 is related to the liquid level in the pressure-regulated chamber, and when the liquid level is regulated, the water discharge can be regulated by regulating the valve 331 on the water outlet pipe 33.
Fig. 3 is a schematic structural view of a rack for placing pressure fine-tuning devices in an embodiment of the present invention.
As shown in FIGS. 1 and 3, the pressure trimmer shelf 4 is also placed in the CO2The cell culture box comprises an upper layer and a lower layer, wherein the upper layer is a placing groove 41, and the lower layer is provided with a push-pull water receiving groove 42. The bottom wall of the housing groove 41 has a through hole 411 through which the water pipe 34 passes. Two guide vanes 43 are symmetrically arranged on the inner wall of the lower layer, edges 421 lapped on the guide vanes are arranged at two ends of the push-pull type water receiving tank, the width of the water receiving tank is the same as the interval between the two guide vanes, and the installation stability of the water receiving tank is improved. The structure of plug-type water receiving tank is more common among the prior art, the utility model discloses only enumerate one of them simplest mode, realize taking out of water receiving tank and water receiving after the installation.
When the pressure fine-tuning device is used, the pressure fine-tuning device is placed in the placing groove, the water outlet pipeline of the pressure fine-tuning device penetrates through the through hole and is positioned above the push-pull type water receiving groove, pressure adjustment is achieved at any time, and the water receiving groove is taken out and poured when water in the water receiving groove is accumulated to a certain degree.
In this embodiment, the negative pressure pump is placed in the CO2Inside the cell culture case, have the display screen, the negative pressure pump that inventor chose when actually carrying out the experiment is the curasys negative pressure pump of CGBIO company.
The principle of the micro-negative pressure cell culture device in the embodiment for realizing the micro-negative pressure environment in the cell culture device is as follows: after the negative pressure pump 5 is started, the cell culture device 2 is in a negative pressure state, and further CO above the pressure regulating cavity 312Flows into the cell culture device 2 and is communicated withThe liquid level height of the pressure cavity is adjusted by adjusting, so that the micro negative pressure in the cell culture device 2 is adjusted. Since the minimum negative pressure value that the negative pressure pump can display is-5 to-10 mmHg, through experiments, the device in the embodiment can realize at least a micro negative pressure of-5 to-10 mmHg, even smaller, but the negative pressure pump cannot display the minimum negative pressure value.
In order to verify the beneficial effect of the micro-negative pressure cell culture device 100 on the cell crawling experiment, the inventors performed a comparative experiment. The human normal skin keratinocytes and fibroblasts were used as the study subjects, the experimental examples were cultured using the apparatus of the present example, and the comparative examples were cultured in a relatively sealed cell culture apparatus using the temperature, humidity, and CO used in the culturing process2The conditions such as concentration are the same. The experimental results are compared as follows:
and observing the cell crawling capacity condition through a scratch experiment. The distance between cells was observed and measured at 0, 12 and 24 hours, respectively, and the procedure was repeated 8 times, and then the cell distance was compared between the control and experimental examples. The specific measurement data are shown in table 1, and the microscopic results of the normal skin keratinocyte and fibroblast scratch test are respectively shown in fig. 4 and fig. 5.
The results indicate that the cell spacing between the two groups at 0 hour after the inoculation of the normal skin keratinocytes is not different, P is more than 0.05, the cell spacing of the experimental examples at 12 and 24 hours is obviously smaller than that of the control example, and P is less than 0.05; after the fibroblast is inoculated, the cell spacing of the groups 0 and 12 hours and 2 is not different, P is more than 0.05, the cell spacing of the experimental example is obviously smaller than that of the control example after 24 hours, and P is less than 0.05.
TABLE 1 cell in vitro culture scratch test cell space Table (X. + -. s, mm) in control and experimental examples
Research proves that the utility model discloses well little negative pressure cell culture device 100 has the promotion effect to the cell crawls, and epidermal cell and the fibroblast of in vitro culture all show stronger ability of crawling. The enhancement of the migration capacity of normal skin keratinocytes occurs earlier, and the migration capacity is enhanced starting from 12 hours after the exposure to the negative pressure environment. Whereas for fibroblast migration capacity, the enhancement occurs 24 hours after receiving the micro-negative pressure environment.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A micro-negative pressure cell culture device, comprising:
CO2a cell culture chamber placed in the CO2A cell culture device, a pressure trimmer and a negative pressure pump in the cell culture box,
the pressure trimmer comprises a pressure adjusting cavity and a water injection pipeline communicated with the side end, wherein a water outlet pipeline is arranged at the bottom end of the pressure adjusting cavity, and a pipe joint is arranged at the top end of the pressure adjusting cavity;
the cell culture device is provided with two tubes, one tube is connected with the negative pressure pump, and the other tube is arranged on the tube connector.
2. The micro negative pressure cell culture device of claim 1, further comprising:
the pressure trimmer placing frame comprises an upper layer and a lower layer, wherein the upper layer is provided with a placing groove with a through hole in the bottom wall, and the lower layer is provided with a push-pull water receiving groove.
3. The micro-negative pressure cell culture device of claim 2, wherein:
the pressure fine-tuning device placing frame is characterized in that two guide vanes are symmetrically arranged on the inner wall of the lower layer of the pressure fine-tuning device placing frame, edges lapped on the guide vanes are arranged at two ends of the push-pull type water receiving tank, and the width of the water receiving tank is the same as the interval between the two guide vanes.
4. The micro-negative pressure cell culture device of claim 1, wherein:
wherein, the cell culture device is a sealed culture device.
5. The micro-negative pressure cell culture device of claim 4, wherein:
wherein the cell culture device comprises a circular bottom frame, a double-layer culture frame arranged on the circular bottom frame, a transparent sealing cover buckled on the circular bottom frame in a sealing way, and a lock catch arranged on the peripheral part of the circular bottom frame and used for locking the transparent sealing cover,
and the circular underframe is provided with two pipes which are respectively connected with the negative pressure pump and the pressure trimmer.
6. The micro-negative pressure cell culture device of claim 2, wherein:
wherein, install the valve on the outlet conduit, this valve is located plug-type water receiving tank top.
7. The micro-negative pressure cell culture device of claim 1, wherein:
and scale marks are arranged on the front wall of the pressure regulating cavity.
8. The micro-negative pressure cell culture device of claim 1, wherein:
wherein, the negative pressure pump is provided with a display screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920949088.XU CN210458217U (en) | 2019-06-24 | 2019-06-24 | Micro-negative pressure cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920949088.XU CN210458217U (en) | 2019-06-24 | 2019-06-24 | Micro-negative pressure cell culture device |
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| Publication Number | Publication Date |
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| CN210458217U true CN210458217U (en) | 2020-05-05 |
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| CN201920949088.XU Expired - Fee Related CN210458217U (en) | 2019-06-24 | 2019-06-24 | Micro-negative pressure cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229752A (en) * | 2019-06-24 | 2019-09-13 | 上海长海医院 | A kind of tiny structure cell culture apparatus |
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2019
- 2019-06-24 CN CN201920949088.XU patent/CN210458217U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229752A (en) * | 2019-06-24 | 2019-09-13 | 上海长海医院 | A kind of tiny structure cell culture apparatus |
| CN110229752B (en) * | 2019-06-24 | 2024-03-19 | 上海长海医院 | Micro negative pressure cell culture device |
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Granted publication date: 20200505 |