CN110229752A - A kind of tiny structure cell culture apparatus - Google Patents
A kind of tiny structure cell culture apparatus Download PDFInfo
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- CN110229752A CN110229752A CN201910571338.5A CN201910571338A CN110229752A CN 110229752 A CN110229752 A CN 110229752A CN 201910571338 A CN201910571338 A CN 201910571338A CN 110229752 A CN110229752 A CN 110229752A
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- tiny structure
- micro actuator
- negative pressure
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- 238000004113 cell culture Methods 0.000 title claims abstract description 45
- 230000001105 regulatory effect Effects 0.000 claims abstract description 16
- 210000004262 dental pulp cavity Anatomy 0.000 claims abstract description 6
- 238000011049 filling Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000007789 sealing Methods 0.000 claims description 7
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 19
- 210000002950 fibroblast Anatomy 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 210000001339 epidermal cell Anatomy 0.000 abstract description 3
- 230000037314 wound repair Effects 0.000 abstract description 2
- 238000000338 in vitro Methods 0.000 abstract 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 12
- 206010052428 Wound Diseases 0.000 description 10
- 208000027418 Wounds and injury Diseases 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 230000037311 normal skin Effects 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 206010021703 Indifference Diseases 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 235000008113 selfheal Nutrition 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Mechanical Engineering (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of tiny structure cell culture apparatus, including CO2Cell incubator is placed on the CO2Cell culture apparatus, pressure micro actuator and negative pressure pump in cell incubator.Wherein, pressure micro actuator includes the filling pipe that side is arranged in pressure regulating cavity and connection, and pressure regulating cavity bottom end is provided with outlet conduit, and top is provided with pipe fitting；Cell culture apparatus is equipped with two root canals, and one connect with negative pressure pump, and another is mounted on pipe fitting.It is proved by experimental study, tiny structure cell culture apparatus is creeped for cell and is proliferated with certain facilitation effect in the present invention, the epidermal cell of in vitro culture and fibroblast show the stronger ability of creeping, and provide and benefit our pursuits for clinic wound repair.
Description
Technical field
The invention belongs to experimental provision technical fields, and in particular to it is a kind of be capable of providing tiny structure cell (epidermis, at fibre
Dimension, vascular endothelial cell etc.) culture apparatus.
Background technique
Negative-pressure sealed drainage technology becomes the open surface of a wound as wound closure, and the surface of a wound can be periodically removed under specified suction function
Sepage, can reduce oedema between tissue, improve tissue microcirculation, promote capillary regeneration, bacterial reproduction is reduced, to promote
The growth of granulation tissue, creates favorable conditions for flap coverage.It is applied in terms of the wound repair of Department of B urn, surgery and orthopaedics
Extensively.
Its negative pressure value of Wound-surface negative pressure drainage technology in the application is -80~-120mmHg at present, in the work of the negative pressure value
Wound exudate can be managed effectively with lower, but growth for epidermis and creeped then without obvious effect, therefore, clinical real
In trampling, skin grafting flap coverage then may be selected after surface of a wound substrate has the growth of fresh granulation or voluntarily climbed to surrounding normal epidermis
Row wound repairing.
After the surface of a wound has the growth that newly granulates in clinical practice, if giving certain tiny structure, the wound healing of patient
Speed and degree are better than the surface of a wound of self-heal.But the minimum sub-atmospheric pressure that negative pressure pump used in the prior art can generate is general
For -30~-50mmHg, strictly and it is not belonging to the scope of tiny structure, therefore even if negative pressure pump is adjusted to minimum, for table
The creeping of chrotoplast still influences little.It is limited by negative pressure pump probe technologies, negative pressure pump manufacturer is difficult to accomplish to show at present
There is the negative pressure value of machine to be further reduced and has reached tiny structure value (- 5~-10mmHg).Therefore, whether tiny structure can promote the surface of a wound
Healing, can not be verified using current negative pressure closed drainage device.
Summary of the invention
The present invention is to carry out to solve above-mentioned technical problem, and tiny structure can be provided for cell culture by providing one kind
The tiny structure cell culture apparatus of environment.In order to realize that purpose, the technical solution adopted in the present invention are as follows:
Tiny structure cell culture apparatus provided by the invention has following technical characteristic: including CO2Cell incubator is placed
In the CO2Cell culture apparatus, pressure micro actuator and negative pressure pump in cell incubator.Wherein, pressure micro actuator includes pressure regulation
The filling pipe of side is arranged in chamber and connection, and pressure regulating cavity bottom end is provided with outlet conduit, and top is provided with pipe fitting；Cell
Incubator is equipped with two root canals, and one connect with negative pressure pump, and another is mounted on pipe fitting.
In the present invention, negative pressure pump is placed on CO2In cell incubator, rather than be placed on outside the reason of be: negative pressure pump
By the CO of absorption2Gas discharges in external environment, and pump is placed on outside, and the carbon dioxide gas cognition of incubator is consumed, needs
Constantly to add carbon dioxide；It is placed on inside cell incubator, opposite can maintain CO in incubator2The balance of gas.
It preferably, further include pressure micro actuator holder in tiny structure cell culture apparatus provided by the invention, including
Upper layer and lower layer, upper layer are provided with the putting groove that bottom wall has through hole, and lower layer is provided with plug-type water receiving tank.
The pressure micro actuator holder is also disposed in CO2In cell incubator, pressure micro actuator is placed in putting groove,
Two outlet conduits both pass through through hole, are located above plug-type water receiving tank, realize and carry out pressure adjusting at any time, in water receiving tank
Water it is accumulative to a certain extent when, water receiving tank taking-up is outwelled.
Preferably, in tiny structure cell culture apparatus provided by the invention, the inner wall of pressure micro actuator holder lower layer
On be symmetrically arranged with two guide cards, the both ends of plug-type water receiving tank are provided with the edge ridden on guide card, the width of water receiving tank with
Interval between two guide cards is identical.The structure of plug-type water receiving tank is more typical in the prior art, and the present invention only lists
One of simplest mode, water receiving after realizing the extraction pouring of water receiving tank and installing.
Preferably, in tiny structure cell culture apparatus provided by the invention, cell culture apparatus includes round chassis, setting
In transparent sealing lid of the Double layer culture frame, seal buckle on round chassis on round chassis, it is arranged in round chassis peripheral portion
To transparent sealing lid into line-locked lock on point, it is provided on round chassis and is connect respectively with negative pressure pump and pressure micro actuator
Two root canals.
Preferably, in tiny structure cell culture apparatus provided by the invention, valve is installed on outlet conduit, the valve
Above plug-type water receiving tank.
Preferably, in tiny structure cell culture apparatus provided by the invention, graduation mark is provided on the antetheca of pressure regulating cavity.
Preferably, in tiny structure cell culture apparatus provided by the invention, negative pressure pump has display screen, and inventor is practical
The negative pressure pump selected when being tested is the curasys negative pressure pump of CGBIO company.
The action and effect of invention
The tiny structure cell culture apparatus provided according to the present invention, due to being provided with pressure micro actuator, by adjusting pressure regulation
Liquid level in chamber realizes formation -5~-10mmHg tiny structure environment in cell culture apparatus, by experimental verification, place
The experimental result being significantly faster than that under non-tiny structure environment with growth rate of creeping of epidermal cell under the tiny structure environment, has
Conducive to simulation exploration is clinically carried out, promote the healing of the surface of a wound.
In addition, the pressure micro actuator structure in the present invention is simple, preparation cost is low, is conducive to laboratory and clinically
It promotes and applies.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of tiny structure cell culture apparatus in the embodiment of the present invention；
Fig. 2 is the structural schematic diagram of pressure micro actuator in the embodiment of the present invention；
Fig. 3 is the structural schematic diagram of pressure micro actuator holder in the embodiment of the present invention；
Fig. 4 is result under the microscope of normal skin horn cell scratch experiment in the embodiment of the present invention；
Fig. 5 is result under the microscope of fibroblast scratch experiment in the embodiment of the present invention.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention will be described in detail.But the following example should not be regarded as to the present invention
The limitation of range.
In the description of the present invention, it should be noted that the orientation or position of the instructions such as term " on ", "lower", "left", "right"
Setting relationship is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of description of the present invention and simplification of the description, rather than
The device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot
It is interpreted as limitation of the present invention.
Fig. 1 is the structural schematic diagram of tiny structure cell culture apparatus in the embodiment of the present invention.
As shown in Figure 1, tiny structure cell culture apparatus 100 includes CO2Cell incubator 1 is placed on the CO2Cell culture
Cell culture apparatus 2, pressure micro actuator 3, pressure micro actuator holder 4 and negative pressure pump 5 in case.
CO2Cell incubator 1 is that biology laboratory often uses incubator, and cell culture apparatus 2 is the cell culture of opposing seal
Device, the transparent sealing lid including round chassis, the Double layer culture frame 21 being arranged on round chassis, seal buckle on round chassis
22, it is arranged on round chassis peripheral portion and 23 is latched into line-locked to transparent sealing lid.Difference is provided on round chassis
Two root canals 24 and 25 being connect with negative pressure pump 5 and pressure micro actuator 3.
Fig. 2 is the structural schematic diagram of pressure micro actuator in the embodiment of the present invention.
As depicted in figs. 1 and 2, pressure micro actuator 3 includes in pressure regulating cavity 31, the filling pipe of 31 side of pressure regulating cavity is arranged in
32, the outlet conduit 33 that pressure regulating cavity bottom end is set, the pipe fitting 34 that pressure regulating cavity top is set.
The front end of pressure regulating cavity 31 is provided with the graduation mark for showing water level, and filling pipe 32 into pressure regulating cavity for infusing
Water.It is related to the liquid level in pressure regulating cavity that tiny structure environment is formed in cell culture apparatus device 2, it, can when liquid level is adjusted
By adjusting the valve 331 on outlet conduit 33, draining is adjusted.
Fig. 3 is the structural schematic diagram of pressure micro actuator holder in the embodiment of the present invention.
As shown in figures 1 and 3, pressure micro actuator holder 4 is also disposed in CO2In cell incubator, including upper layer and lower layer,
Upper layer is putting groove 41, and lower layer is provided with plug-type water receiving tank 42.The bottom wall of putting groove 41, which has, allows outlet conduit 34 to pass through
Through hole 411.Two guide cards 43 are symmetrically arranged on the inner wall of lower layer, the both ends of plug-type water receiving tank, which are provided with, to be ridden on guide card
Edge 421, the interval between the width of water receiving tank and two guide cards is identical, increases the installation stability of water receiving tank.It is plug-type
The structure of water receiving tank is more typical in the prior art, and the present invention only lists one of simplest mode, realizes water receiving
Water receiving after the extraction of slot pours and installs.
In use, pressure micro actuator is placed in putting groove, outlet conduit passes through through hole, is located at plug-type water receiving tank
Top is realized and carries out pressure adjusting at any time, and when the water in water receiving tank adds up to a certain extent, water receiving tank taking-up is outwelled.
In the present embodiment, negative pressure pump is placed on CO2Inside cell incubator, display screen is had, the practical progress of inventor is real
The negative pressure pump selected when testing is the curasys negative pressure pump of CGBIO company.
Tiny structure cell culture apparatus in the present embodiment realizes that the principle of tiny structure environment in cell culture apparatus is as follows: negative
After press pump 5 is opened, so that negative pressure state is in cell culture apparatus 2, so that the CO of 31 top of pressure regulating cavity2Flow into cell training
It supports in device 2, by adjusting pressure regulating cavity liquid level, realizes the adjusting of tiny structure in cell culture apparatus 2.Since negative pressure pump can be shown
The minimum negative pressure shown is -5~-10mmHg, and by experiment, -5~-10mmHg is at least may be implemented in the device in the present embodiment
Tiny structure, even more small, only negative pressure pump can not carry out showing.
It creeps the advantageous effects of experiment to verify tiny structure cell culture apparatus 100 in the present invention for cell, invention
People has carried out comparative experiments.Using people's normal skin horn cell and fibroblast as research object, experimental example uses this reality
The device applied in example is cultivated, and reference examples are cultivated in the cell culture apparatus of opposing seal, employed in incubation
Temperature, humidity, CO2The conditions such as concentration are identical.Experimental result comparison is as follows:
Cell is observed by scratch experiment to creep capabilities might.Respectively at the 0th, 12,24 hour observation, measurement iuntercellular away from
From operation being repeated 8 times, then by reference examples and experimental example iuntercellular away from being compared.Specific measurement data is shown in Table 1, normal skin
Result is shown in Fig. 4 and Fig. 5 respectively under the microscope of skin horn cell and fibroblast scratch experiment.
As a result it prompting, the 0th hour iuntercellular between two groups is away from indifference after the inoculation of normal skin horn cell, P > 0.05,
12,24 hours experimental example iuntercellulars are away from being significantly less than reference examples, P < 0.05；It is the 0th, 12 hour 2 groups thin after fibroblast inoculation
Intercellular is away from indifference, and 0.05,24 hours experimental example iuntercellulars of P > are away from being significantly less than reference examples, P < 0.05.
Cell injuring model scratch experiment iuntercellular is away from table (X ± s, mm) in 1 reference examples of table and experimental example
Research has shown that tiny structure cell culture apparatus 100 creeps with facilitation effect for cell in the present invention, external to train
Feeding epidermal cell and fibroblast shows the stronger ability of creeping.For normal skin keratinocyte cell migration ability
Enhancing occurs earlier, in receiving the transfer ability enhancing for subnormal ambient 12 hours.And for migration of fibroblast cells energy
Power, enhancing betide receive tiny structure environment after 24 hours.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent defines.
Claims (8)
1. a kind of tiny structure cell culture apparatus characterized by comprising
CO2Cell incubator is placed on the CO2Cell culture apparatus, pressure micro actuator and negative pressure pump in cell incubator,
Wherein, the pressure micro actuator includes the filling pipe that side is arranged in pressure regulating cavity and connection, the pressure regulating cavity bottom end
It is provided with outlet conduit, top is provided with pipe fitting；
The cell culture apparatus is equipped with two root canals, and one connect with the negative pressure pump, and another is mounted on the pipe fitting.
2. tiny structure cell culture apparatus according to claim 1, which is characterized in that further include:
Pressure micro actuator holder, including upper layer and lower layer, upper layer are provided with the putting groove that bottom wall has through hole, and lower layer is provided with
Plug-type water receiving tank.
The experimental provision 3. tiny structure formula cell according to claim 2 is creeped, it is characterised in that:
Wherein, two guide cards, the plug-type water receiving tank are symmetrically arranged on the inner wall of the pressure micro actuator holder lower layer
Both ends be provided with the edge ridden on the guide card, the interval between the width of water receiving tank and two guide cards is identical.
4. tiny structure cell culture apparatus according to claim 1, it is characterised in that:
Wherein, the cell culture apparatus is sealing incubator.
5. tiny structure cell culture apparatus according to claim 4, it is characterised in that:
Wherein, the cell culture apparatus includes that round chassis, the Double layer culture frame being arranged on the round chassis, seal buckle exist
Transparent sealing lid, setting on the circle chassis lock the transparent sealing lid on the round chassis peripheral portion
Fixed lock,
Two root canals connecting respectively with the negative pressure pump and pressure micro actuator are provided on the circle chassis.
6. tiny structure cell culture apparatus according to claim 2, it is characterised in that:
Wherein, valve is installed on the outlet conduit, which is located above the plug-type water receiving tank.
7. tiny structure cell culture apparatus according to claim 1, it is characterised in that:
Wherein, graduation mark is provided on the antetheca of the pressure regulating cavity.
8. tiny structure cell culture apparatus according to claim 1, it is characterised in that:
Wherein, the negative pressure pump has display screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910571338.5A CN110229752B (en) | 2019-06-24 | 2019-06-24 | Micro negative pressure cell culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910571338.5A CN110229752B (en) | 2019-06-24 | 2019-06-24 | Micro negative pressure cell culture device |
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| CN110229752A true CN110229752A (en) | 2019-09-13 |
| CN110229752B CN110229752B (en) | 2024-03-19 |
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