JP2017023131A - Cell culture apparatus - Google Patents
Cell culture apparatus Download PDFInfo
- Publication number
- JP2017023131A JP2017023131A JP2016105714A JP2016105714A JP2017023131A JP 2017023131 A JP2017023131 A JP 2017023131A JP 2016105714 A JP2016105714 A JP 2016105714A JP 2016105714 A JP2016105714 A JP 2016105714A JP 2017023131 A JP2017023131 A JP 2017023131A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- culture
- supply
- holding means
- negative pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
本発明は、培養バッグ（細胞培養バッグ）内の培地を自動交換可能な細胞培養装置に関するものである。 The present invention relates to a cell culture apparatus capable of automatically replacing a culture medium in a culture bag (cell culture bag).
近年、幹細胞研究や再生医療の進展に伴い、臨床用途の細胞を大量に調製することが要求されている。臨床用途の細胞の調製には厳しい基準に適った環境での作業が求められ、そのため、作業者が作業空間に立ち入る際には使い捨ての作業着に着替えたりするなど大変な手間とコストが発生している。また、作業者による作業工程は培養系が汚染される機会となっている。したがって、作業者が作業空間に立ち入り作業をする回数をできる限り少なくし、可能な作業については人手によらず自動で行うことが求められている。
細胞を培養するには定期的に培地（細胞培養液）の交換が必要となるが、培地交換は培養系の汚染（コンタミネーション）のリスクを伴い、可能な限り人手を介さずに自動で行えることが望ましい。自動で培地を交換するシステムとして、搬送ロボットにより培養容器をインキュベータと培地交換ロボットの間で移動させるシステムが知られている（特許文献１）。
In recent years, with the progress of stem cell research and regenerative medicine, it is required to prepare a large number of cells for clinical use. Preparation of cells for clinical use requires work in an environment that meets strict standards, and therefore, when workers enter the work space, they change clothes into disposable work clothes, which requires great effort and cost. ing. In addition, the work process by the worker is an opportunity to contaminate the culture system. Therefore, it is required to minimize the number of times that the worker enters the work space as much as possible, and to perform the possible work automatically without human intervention.
To culture cells, it is necessary to periodically change the medium (cell culture medium). However, medium replacement involves the risk of contamination of the culture system and can be performed automatically without human intervention as much as possible. It is desirable. As a system for automatically exchanging a medium, a system in which a culture container is moved between an incubator and a medium exchanging robot by a transfer robot is known (Patent Document 1).
臨床用途の細胞の調製には厳しい基準が設定されており、培養系が汚染されるリスクはなるべく排除する必要があるため、培地交換の過程を自動化することが有利となるが、特許文献１のような自動培地交換システムでは、搬送ロボット等を採用するためシステムが非常に複雑となり、システムエラーを起こすリスクが高くなる。また、培養容器をインキュベータから出し入れするため、培養容器内の細胞に温度変化のよるストレスがかかることになる。それをさけるためにシステムをインキュベータ内に設置すると、インキュベータ内は多湿の環境にあるため、機械的、電気的な構造物がトラブルを起こすリスクがあり、インキュベータ内のシステム構成物はなるべく簡単な構成が好ましい。 Strict standards are set for the preparation of cells for clinical use, and it is necessary to eliminate the risk of contamination of the culture system as much as possible. Therefore, it is advantageous to automate the medium exchange process. In such an automatic medium change system, since a transfer robot or the like is employed, the system becomes very complicated and the risk of causing a system error increases. In addition, since the culture container is taken in and out of the incubator, the cells in the culture container are subjected to stress due to temperature changes. If the system is installed in the incubator to avoid it, the incubator is in a humid environment, so there is a risk that mechanical and electrical structures will cause trouble, and the system components in the incubator are configured as simple as possible. Is preferred.
本発明は、上述した事情に鑑みてなされたものであって、細胞培養空間における培養バッグ（細胞培養バッグ）内の培地を簡易に自動で交換することができるとともに、装置を簡単な構成とすることでシステムトラブルの発生リスクを軽減できる細胞培養装置を提供することを目的としている。 The present invention has been made in view of the above-described circumstances, and can easily and automatically change the medium in the culture bag (cell culture bag) in the cell culture space, and has a simple configuration. Therefore, an object of the present invention is to provide a cell culture device that can reduce the risk of system trouble.
上記目的を達成するために、本発明は以下の手段を提供する。本発明の一態様は、
細胞を培養するための培地を保持する培地保持手段と、
該培地保持手段と連結し、前記培地保持手段から排出された培地を培養バッグに供給する培地供給手段と、
該培地供給手段と接続する供給口と、内部から培地を排出するための排出口を備えた培養バッグと、
前記培養バッグの前記排出口に陰圧を供給する陰圧供給手段と、
前記培養バッグの前記排出口からの培地を保持する廃液保持手段を備えた細胞培養装置を提供する。
In order to achieve the above object, the present invention provides the following means. One embodiment of the present invention provides:
Medium holding means for holding a medium for culturing cells;
Medium supply means connected to the medium holding means and supplying the culture medium discharged from the medium holding means to the culture bag;
A culture bag having a supply port connected to the medium supply means, and a discharge port for discharging the medium from the inside;
Negative pressure supply means for supplying negative pressure to the outlet of the culture bag;
Provided is a cell culture device provided with waste liquid holding means for holding a culture medium from the outlet of the culture bag.
本態様によれば、簡単な構成によって培養バッグの培地を交換することができる。 According to this aspect, the culture medium in the culture bag can be exchanged with a simple configuration.
また、上記態様においては、前記培地供給手段が、培養バッグへ培地を供給する速度を調整する供給速度調整手段を備えていても良い。このことにより、培養バッグへの培地の供給速度を任意に変えることができるので、培地の排出速度より供給速度を遅く調整することで培養バッグ内の培地の交換効率を上げることができる。 Moreover, in the said aspect, the said culture medium supply means may be provided with the supply speed adjustment means which adjusts the speed | rate which supplies a culture medium to a culture bag. As a result, the supply rate of the medium to the culture bag can be arbitrarily changed, so that the exchange efficiency of the medium in the culture bag can be increased by adjusting the supply rate slower than the discharge rate of the medium.
また、上記態様においては、前記培養バッグの前記排出口の口径が、前記供給口の口径より大きく設定しても良い。このことにより、培地の培養バッグからの排出速度より培養バッグへの供給速度を遅くすることができ、培養バッグ内の培地の交換効率を上げることができる。 Moreover, in the said aspect, you may set the diameter of the said discharge port of the said culture bag larger than the diameter of the said supply port. Thereby, the supply speed to the culture bag can be made slower than the discharge speed of the culture medium from the culture bag, and the replacement efficiency of the culture medium in the culture bag can be increased.
また、上記態様においては、培地を保存に適した温度に維持し前記培地保持手段に培地を供給する培地保存手段と、前記培地保持手段内の培地の量を検出する液量センサと、をさらに備え、前記培地保存手段から前記培地保持手段に供給された培地量が所定の量に達したときに、前記液量センサがそれを検出し、前記陰圧供給手段を作動させても良い。このことにより、簡単な構成によって、所望の周期で培地を断続的に供給、排出することができる。 In the above aspect, the medium storage means for maintaining the medium at a temperature suitable for storage and supplying the medium to the medium holding means, and the liquid amount sensor for detecting the amount of the medium in the medium holding means, In addition, when the amount of the medium supplied from the medium storage means to the medium holding means reaches a predetermined amount, the liquid amount sensor may detect it and operate the negative pressure supply means. Accordingly, the medium can be intermittently supplied and discharged at a desired cycle with a simple configuration.
また、上記態様においては、培地を保存に適した温度に維持し前記培地保持手段に培地を供給する培地保存手段と、前記培地保持手段内の培地の量を検出する液量センサと、をさらに備え、前記培地保存手段から前記培地保持手段に供給された培地量が所定の量に達したときに、前記液量センサがそれを検出し、前記陰圧供給手段および前記供給速度調整手段を作動させても良い。このことにより、簡単な構成によって、所望の周期で培地を断続的に供給、排出することができる。 In the above aspect, the medium storage means for maintaining the medium at a temperature suitable for storage and supplying the medium to the medium holding means, and the liquid amount sensor for detecting the amount of the medium in the medium holding means, And when the amount of medium supplied from the medium storage means to the medium holding means reaches a predetermined amount, the liquid amount sensor detects it and activates the negative pressure supply means and the supply speed adjustment means You may let them. Accordingly, the medium can be intermittently supplied and discharged at a desired cycle with a simple configuration.
また、上記態様においては、前記培養バッグの前記排出口からの培地が、前記廃液保持手段内の空間を滴下されても良い。このことにより、培地の逆流を防ぐことができ、培養バッグ内が汚染される（コンタミネーションが起こる）ことを防止することができる。 Moreover, in the said aspect, the culture medium from the said discharge port of the said culture bag may be dripped at the space in the said waste liquid holding means. As a result, the back flow of the medium can be prevented, and the inside of the culture bag can be prevented from being contaminated (contamination occurs).
本発明によれば、簡単なシステムの構成によって培養バッグの培地を交換することができるので、システムエラーのリスクを軽減することができ、エラーにより培養条件に影響を及ぼしてしまうリスクを回避することができる。 According to the present invention, since the culture medium in the culture bag can be replaced with a simple system configuration, the risk of system errors can be reduced, and the risk of affecting the culture conditions due to errors can be avoided. Can do.
本発明の実施形態に係る細胞培養装置について、図面を参照して以下に説明する。
（第１実施形態）
本実施形態に係る細胞培養装置１００は、図１に示される構成の装置であり、インキュベータ内に設置された培養バッグ（細胞培養バッグ）内の培地を交換する装置である。
A cell culture device according to an embodiment of the present invention will be described below with reference to the drawings.
(First embodiment)
A cell culture device 100 according to the present embodiment is a device having the configuration shown in FIG. 1, and is a device for exchanging a medium in a culture bag (cell culture bag) installed in an incubator.
培地保持手段３は、インキュベータ１の内部に設置されており、内部に培地（細胞培養液）を保持するためのものである。培地の温度をインキュベータ１の内部の温度（例えば３７℃）に維持する。 The medium holding means 3 is installed inside the incubator 1 and is for holding a medium (cell culture solution) inside. The temperature of the medium is maintained at the temperature inside the incubator 1 (for example, 37 ° C.).
培地保持手段３で細胞培養に適したインキュベータ１の内部の温度（例えば３７℃）に温められた培地は、インキュベータ１内で培地供給手段４を介して培養バッグ２に供給される。培地保持手段３は培地供給手段４に培地を排出する排出口３ａを備えている。排出口３ａの設置場所は、内部の培地を最後まで排出できる場所、つまり、培地保持手段３の底面または、側面の下部が好ましい。 The medium heated to a temperature inside the incubator 1 suitable for cell culture (for example, 37 ° C.) by the medium holding means 3 is supplied to the culture bag 2 through the medium supply means 4 in the incubator 1. The culture medium holding means 3 includes a discharge port 3 a for discharging the culture medium to the culture medium supply means 4. The place where the discharge port 3a is installed is preferably a place where the internal medium can be discharged to the end, that is, the bottom face of the medium holding means 3 or the lower part of the side face.
培地供給手段４は、培地保持手段３の排出口３ａと培養バッグ２の供給口２ａをつなぐチューブ等の管状部材を備え、複数の培養バッグ２を使用する場合は、培地保持手段３の排出口３ａからの管状部材４ａが分岐部４ｃで複数の管状部材４ｂに分岐し、それぞれが異なる培養バッグ２の供給口２ａに接続している。 The culture medium supply means 4 includes a tubular member such as a tube connecting the discharge port 3a of the culture medium holding means 3 and the supply port 2a of the culture bag 2, and when using a plurality of culture bags 2, the discharge port of the culture medium holding means 3 The tubular member 4a from 3a is branched into a plurality of tubular members 4b at the branch portion 4c, and each is connected to the supply port 2a of the culture bag 2 different from each other.
培養バッグ２は培地供給手段４の管状部材４ｂと接続する供給口２ａと、培養バッグ２外に培地を排出する排出口２ｂを有している。供給口２ａと排出口２ｂの設置位置は任意であるが、互いに可能な限り距離を離して設置した方が培地の交換効率を上げることができる。
また、排出口２ｂの口径（または断面面積）は供給口２ａの口径（または断面面積）より大きいことが好ましい。こうすることで、新しい培地の供給より早く培養バッグ２内の古い培地が排出されるようになり、培地の交換効率を上げることができる。
なお、培養バッグ２は接着細胞用の培養バッグであり、細胞が接着しやすいように表面加工がされていることが好ましい。
The culture bag 2 has a supply port 2 a connected to the tubular member 4 b of the medium supply means 4 and a discharge port 2 b for discharging the medium outside the culture bag 2. The installation positions of the supply port 2a and the discharge port 2b are arbitrary, but the medium replacement efficiency can be increased by installing the supply port 2a and the discharge port 2b as far as possible from each other.
Moreover, it is preferable that the diameter (or cross-sectional area) of the discharge port 2b is larger than the diameter (or cross-sectional area) of the supply port 2a. By doing so, the old medium in the culture bag 2 is discharged earlier than the supply of a new medium, and the medium replacement efficiency can be increased.
The culture bag 2 is a culture bag for adherent cells, and is preferably surface-treated so that the cells can easily adhere.
培養バッグ２内の培地は、培地排出手段により排出口２ｂから排出される。培地排出手段は、廃液保持手段５と陰圧供給手段６を備えている。
廃液保持手段５は培養バッグ２からのチューブ等の管状部材と接続する廃液供給口５ａと、廃液保持手段５外に培地を排出する廃液排出口５ｂを有している。廃液供給口５ａは廃液保持手段５の上面に設置されており、廃液供給口５ａから供給された培地は廃液保持手段５内の空間を滴下され、廃液排出口５ｂより排出される。このように、培地が廃液保持手段５内の空間を滴下されることで、培地の逆流を防ぐことができ、培養バッグ２内が汚染される（コンタミネーションが起こる）ことを防止することができる。
廃液排出口５ｂは陰圧供給手段６に接続されている。
The culture medium in the culture bag 2 is discharged from the discharge port 2b by the medium discharge means. The medium discharge means includes a waste liquid holding means 5 and a negative pressure supply means 6.
The waste liquid holding means 5 has a waste liquid supply port 5 a connected to a tubular member such as a tube from the culture bag 2 and a waste liquid discharge port 5 b for discharging the culture medium outside the waste liquid holding means 5. The waste liquid supply port 5a is installed on the upper surface of the waste liquid holding means 5, and the medium supplied from the waste liquid supply port 5a is dropped in the space in the waste liquid holding means 5 and discharged from the waste liquid discharge port 5b. As described above, the medium is dropped in the space in the waste liquid holding means 5, so that the back flow of the medium can be prevented and the inside of the culture bag 2 can be prevented from being contaminated (contamination occurs). .
The waste liquid discharge port 5 b is connected to the negative pressure supply means 6.
陰圧供給手段６としては、図２に示すようなポンプ６１と廃液容器６２を備えた手段を例示することができる。陰圧供給手段６の吸引口６３を廃液保持手段５の廃液排出口５ｂと接続して廃液保持手段５内を陰圧にし、培養バッグ２の排出口２ｂを陰圧にすることで培養バッグ２から培地を吸引することが可能である。
陰圧供給手段のポンプとしてペリスタポンプ等の送液ポンプを使用しても良い。この場合、送液ポンプを吸引口６３の管状部材に設置しても良い。
Examples of the negative pressure supply means 6 include a means including a pump 61 and a waste liquid container 62 as shown in FIG. The suction port 63 of the negative pressure supply means 6 is connected to the waste liquid discharge port 5b of the waste liquid holding means 5, so that the inside of the waste liquid holding means 5 has a negative pressure, and the discharge port 2b of the culture bag 2 has a negative pressure. It is possible to aspirate the culture medium.
A liquid feed pump such as a peristaltic pump may be used as a pump for the negative pressure supply means. In this case, a liquid feed pump may be installed on the tubular member of the suction port 63.
次に、本実施形態に係る細胞培養装置１００を用いて培地を交換する手順について一例を説明する。
本装置のユーザは、まず培地及び細胞（接着細胞）が入った培養バッグ２を用意し、インキュベータ１内で培養バッグ２の供給口２ａを培地供給手段４の管状部材４ｂに、培養バッグ２の排出口２ｂを管状部材５ｃを介して廃液保持手段５の廃液供給口５ａに接続する。
培地交換が必要になった際、ユーザはまず陰圧供給手段６を作動させ、廃液保持手段５内を陰圧にする。このことにより、培養バッグ２内の培地が管状部材５ｃを介して廃液保持手段５内に吸引される。同時に、培養バッグ２内も陰圧になり、培地保持手段３内の培地が培地供給手段４を介して培養バッグ２内に供給される。
ユーザは培養バッグ２内の培地が適量排出された段階で陰圧供給手段６を停止する。このことにより、廃液保持手段５内の陰圧が解除され、培養バッグ２内からの培地の排出が停止する。また、培養バッグ２内が常圧に戻ると培養バッグ２内への培地の供給も停止する。
Next, an example of the procedure for exchanging the medium using the cell culture device 100 according to the present embodiment will be described.
The user of this apparatus first prepares a culture bag 2 containing a culture medium and cells (adherent cells). In the incubator 1, the supply port 2a of the culture bag 2 is connected to the tubular member 4b of the culture medium supply means 4, and the culture bag 2 The discharge port 2b is connected to the waste liquid supply port 5a of the waste liquid holding means 5 through the tubular member 5c.
When the medium needs to be replaced, the user first operates the negative pressure supply means 6 to make the waste liquid holding means 5 have a negative pressure. Thereby, the culture medium in the culture bag 2 is sucked into the waste liquid holding means 5 through the tubular member 5c. At the same time, the culture bag 2 also has a negative pressure, and the medium in the medium holding means 3 is supplied into the culture bag 2 via the medium supply means 4.
The user stops the negative pressure supply means 6 when an appropriate amount of medium in the culture bag 2 is discharged. As a result, the negative pressure in the waste liquid holding means 5 is released, and the discharge of the medium from the culture bag 2 is stopped. In addition, when the inside of the culture bag 2 returns to normal pressure, the supply of the medium into the culture bag 2 is also stopped.
本実施形態において、陰圧供給手段６を遠隔的に操作する制御部を備えていても良い。こうすることで、ユーザは、例えば図示しないモニタリングシステムで遠隔監視しながら、所望のタイミングで培地交換をすることができる。
また、あらかじめ設定したプログラムにより制御部が陰圧供給手段６を自動制御しても良い。
In this embodiment, you may provide the control part which operates the negative pressure supply means 6 remotely. By doing so, the user can change the medium at a desired timing while remotely monitoring with a monitoring system (not shown), for example.
Further, the negative pressure supply means 6 may be automatically controlled by the control unit according to a preset program.
（第２実施形態）
本実施形態に係る細胞培養装置２００は、図３に示される構成の装置であり、培地供給手段４の管状部材に供給速度調整手段１０を備えた点で第１実施形態と異なっている。それ以外は、第１実施形態と同様である。
(Second Embodiment)
A cell culture device 200 according to this embodiment is a device having the configuration shown in FIG. 3, and is different from the first embodiment in that a supply speed adjusting means 10 is provided on the tubular member of the medium supply means 4. The rest is the same as in the first embodiment.
供給速度調整手段１０は培地供給手段４の管状部材（チューブ等）に配置されており、管状部材に外力を加えて変形させ、管状部材内腔の断面積を小さくすることで溶液の流量を制限して流速を抑制する。逆に外力を解除すると、管状部材の弾性力により管状部材が元の状態に戻り、流速を上げることができる。このように、供給速度調整手段１０は管状部材に対する外力の強弱により管状部材内に流れる溶液の流速を調整する。供給速度調整手段１０による管状部材への外力の加え方について図４に例を示す。（ａ）は２枚の板状部材２１により管状部材２０を挟む例、（ｂ）は複数の球状（または円柱状）部材２２により貫通穴２３に通した管状部材２０を挟む例、（ｃ）はシャッター状部材２４で貫通穴２５に通した管状部材２０を挟む例、（ｄ）は管状部材２０を通した貫通穴２６の内径が小さくなり管状部材を変形させる例である。これ以外でも外力を加えて管状部材を変形させることができる機構であれば良い。 The supply speed adjusting means 10 is disposed on a tubular member (tube or the like) of the culture medium supply means 4 and deforms the tubular member by applying an external force to limit the flow rate of the solution by reducing the cross-sectional area of the tubular member lumen. To suppress the flow rate. Conversely, when the external force is released, the tubular member returns to its original state by the elastic force of the tubular member, and the flow velocity can be increased. In this way, the supply speed adjusting means 10 adjusts the flow rate of the solution flowing in the tubular member by the strength of the external force on the tubular member. An example of how external force is applied to the tubular member by the supply speed adjusting means 10 is shown in FIG. (A) is an example in which the tubular member 20 is sandwiched between two plate-like members 21, (b) is an example in which the tubular member 20 passed through the through hole 23 is sandwiched between a plurality of spherical (or cylindrical) members 22, and (c). Is an example in which the tubular member 20 passed through the through hole 25 is sandwiched by the shutter-like member 24, and (d) is an example in which the inner diameter of the through hole 26 through the tubular member 20 is reduced and the tubular member is deformed. Other than this, any mechanism that can deform the tubular member by applying an external force may be used.
ユーザは供給速度調整手段１０により培養バッグ２への培地供給速度を調整することができる（培地供給速度を０にして供給を止めることもできる）。培養バッグ２へ培地が供給される速度を培養バッグ２から培地が排出される速度より遅くすれば、古い培地と新しい培地の交換効率をあげることができる。 The user can adjust the medium supply speed to the culture bag 2 by the supply speed adjusting means 10 (the medium supply speed can be set to 0 to stop the supply). If the rate at which the medium is supplied to the culture bag 2 is slower than the rate at which the medium is discharged from the culture bag 2, the exchange efficiency of the old medium and the new medium can be increased.
本実施形態に係る培地交換装置２００を用いて培地を交換する手順については第１実施形態と同様であるが、本実施形態においては、ユーザは供給速度調整手段１０により培養バッグ２への培地供給速度を調整することができる。 The procedure for exchanging the medium using the medium exchanging apparatus 200 according to the present embodiment is the same as that in the first embodiment, but in this embodiment, the user supplies the medium to the culture bag 2 by the supply speed adjusting means 10. The speed can be adjusted.
本実施形態において、陰圧供給手段６および供給速度調整手段１０を遠隔的に操作する制御部を備えていても良い。こうすることで、ユーザは、例えば図示しないモニタリングシステムで遠隔監視しながら、所望のタイミングで培地交換をすることができる。
また、あらかじめ設定したプログラムにより制御部が陰圧供給手段６および供給速度調整手段１０を自動制御しても良い。
In the present embodiment, a controller for remotely operating the negative pressure supply means 6 and the supply speed adjustment means 10 may be provided. By doing so, the user can change the medium at a desired timing while remotely monitoring with a monitoring system (not shown), for example.
Further, the controller may automatically control the negative pressure supply means 6 and the supply speed adjustment means 10 by a preset program.
（第３実施形態）
本実施形態に係る細胞培養装置３００は、図５に示される構成の装置であり、培地保存手段３１を備え、培地保持手段３内に液量センサ３２を備えた点で第１実施形態と異なっている。それ以外は、第１実施形態と同様である。
(Third embodiment)
The cell culture device 300 according to the present embodiment is a device having the configuration shown in FIG. 5, and is different from the first embodiment in that it includes a medium storage unit 31 and a liquid amount sensor 32 in the medium holding unit 3. ing. The rest is the same as in the first embodiment.
培地保存手段３１は培地保存容器３１ａを備え、チューブ等の管状部材３１ｃを介して培地保持手段３の開口部（供給口３ｂ）に接続している。管状部材３１ｃにはペリスタポンプ等の送液ポンプ３１ｄを備えられ、培地保存容器３１ａ内の培地を培地保持手段３に供給することができる。培地保存容器３１ａ内の培地は、温度調節手段３１ｂにより培地の保存に適した温度（例えば４℃）に維持されている。
培地保持手段３の供給口３ｂの設置位置は任意であるが、供給された培地が培地保持手段３内の空間を滴下するように、培地保持手段３の上面部にあることが好ましい。このことにより、培地の逆流を防ぐことができ、培地保存容器３１ａ内が汚染される（コンタミネーションが起こる）ことを防止することができる。
The culture medium storage means 31 includes a culture medium storage container 31a, and is connected to the opening (supply port 3b) of the culture medium holding means 3 via a tubular member 31c such as a tube. The tubular member 31 c is provided with a liquid feed pump 31 d such as a peristaltic pump, and the medium in the medium storage container 31 a can be supplied to the medium holding means 3. The medium in the medium storage container 31a is maintained at a temperature suitable for storage of the medium (for example, 4 ° C.) by the temperature adjusting means 31b.
Although the installation position of the supply port 3b of the culture medium holding means 3 is arbitrary, it is preferable that the supply medium is on the upper surface of the culture medium holding means 3 so that the supplied culture medium drops in the space in the culture medium holding means 3. Thereby, the back flow of the culture medium can be prevented, and the inside of the culture medium storage container 31a can be prevented from being contaminated (contamination occurs).
液量センサ３２は培地保持手段３内の培地量を検出するセンサであり、培地保持手段３内の培地量が所定量に達した際にそれを検知し、陰圧供給手段６へ情報を送ることで陰圧供給手段６を作動させることができる。陰圧供給手段６は作動開始してから所定時間経過後に停止するようになっていても良いし、液量センサ３２が培地保持手段３内の培地量が所定量まで減少した際にそれを検知し、陰圧供給手段６へ情報を送ることで陰圧供給手段６を停止させても良い。液量センサ３２から陰圧供給手段６への情報の伝達は有線でも無線でも良い。 The liquid amount sensor 32 is a sensor for detecting the amount of medium in the medium holding means 3, detects when the amount of medium in the medium holding means 3 reaches a predetermined amount, and sends information to the negative pressure supply means 6. Thus, the negative pressure supply means 6 can be operated. The negative pressure supply means 6 may stop after a predetermined time has elapsed since the start of operation, or the liquid amount sensor 32 detects when the medium amount in the medium holding means 3 has decreased to a predetermined amount. Then, the negative pressure supply means 6 may be stopped by sending information to the negative pressure supply means 6. Transmission of information from the liquid amount sensor 32 to the negative pressure supply means 6 may be wired or wireless.
液量センサ３２の例としては、光学的や電気的に培地保持手段３内の培地上面の液面を検出できるセンサを挙げることができる。所定位置に設置した電極に培地が接触することで生じる通電を検出したり、静電容量の変化で液面位置を検出したり、液面からの反射光により光学的に液面位置を検出したり、超音波や電波を液面に反射させて反射時間を検出したりするセンサなどがある。また、培地保持手段３内の培地にフロートを浮かべてフロートの位置を検出したり、培地保持手段３の重量変化（圧力変化）を検出したりしても良い。 As an example of the liquid amount sensor 32, a sensor that can optically or electrically detect the liquid level on the upper surface of the medium in the medium holding means 3 can be mentioned. Detects energization that occurs when the medium comes in contact with the electrode installed at a predetermined position, detects the liquid surface position by changing the capacitance, and optically detects the liquid surface position by reflected light from the liquid surface. Or a sensor that detects the reflection time by reflecting ultrasonic waves or radio waves to the liquid surface. Alternatively, the float may be floated on the medium in the medium holding means 3 to detect the position of the float, or the weight change (pressure change) of the medium holding means 3 may be detected.
次に、本実施形態に係る培地交換装置３００を用いて培地を交換する手順について一例を説明する。
本装置のユーザは、まず培地及び細胞（接着細胞）が入った培養バッグ２を用意し、インキュベータ１内で培養バッグ２の供給口２ａを培地供給手段４の管状部材４ｂに、培養バッグ２の排出口２ｂを管状部材５ｃを介して廃液保持手段５の廃液供給口５ａに接続する。
培地保存手段３１を培地保持手段３の供給口３ｂに接続し、送液ポンプ３１ｄを作動させて培地保持手段３へ培地の供給を開始する。培地保持手段３へ供給された培地はインキュベータ１内の温度（細胞培養に適した温度）に暖められる。培地保持手段３内の培地が所定量に達すると液量センサ３２がそれを検知し、陰圧供給手段６へ情報を送ることで陰圧供給手段６を作動させ、廃液保持手段５内を陰圧にする（液量センサ３２が作動する前は、陰圧供給手段６は停止した状態となっている）。このことにより、培養バッグ２内の培地が管状部材５ｃを介して廃液保持手段５内に吸引される。同時に、培養バッグ２内も陰圧になり、培地保持手段３内の培地が培地供給手段４を介して培養バッグ２内に供給される。
陰圧供給手段６は作動開始してから所定時間経過後に停止する。このことにより、廃液保持手段５内の陰圧が解除され、培養バッグ２内からの培地の排出が停止する。また、培養バッグ２内が常圧に戻ると培養バッグ２内への培地の供給も停止し、培地保持手段３内の培地量が増加し始める。培地保持手段３内の培地が所定量に達すると液量センサ３２がそれを検知し、上記ステップを繰り返す。
Next, an example of the procedure for exchanging the medium using the medium exchanging apparatus 300 according to the present embodiment will be described.
The user of this apparatus first prepares a culture bag 2 containing a culture medium and cells (adherent cells). In the incubator 1, the supply port 2a of the culture bag 2 is connected to the tubular member 4b of the culture medium supply means 4, and the culture bag 2 The discharge port 2b is connected to the waste liquid supply port 5a of the waste liquid holding means 5 through the tubular member 5c.
The culture medium storage means 31 is connected to the supply port 3b of the culture medium holding means 3, and the liquid feed pump 31d is operated to start supplying the culture medium to the culture medium holding means 3. The medium supplied to the medium holding means 3 is warmed to the temperature in the incubator 1 (temperature suitable for cell culture). When the culture medium in the culture medium holding means 3 reaches a predetermined amount, the liquid amount sensor 32 detects it and sends information to the negative pressure supply means 6 to operate the negative pressure supply means 6, and the waste liquid holding means 5 is negatively charged. The negative pressure supply means 6 is stopped before the liquid amount sensor 32 is activated. Thereby, the culture medium in the culture bag 2 is sucked into the waste liquid holding means 5 through the tubular member 5c. At the same time, the culture bag 2 also has a negative pressure, and the medium in the medium holding means 3 is supplied into the culture bag 2 via the medium supply means 4.
The negative pressure supply means 6 stops after a predetermined time has elapsed since the start of operation. As a result, the negative pressure in the waste liquid holding means 5 is released, and the discharge of the medium from the culture bag 2 is stopped. Further, when the inside of the culture bag 2 returns to normal pressure, the supply of the medium into the culture bag 2 is also stopped, and the amount of medium in the medium holding means 3 begins to increase. When the medium in the medium holding means 3 reaches a predetermined amount, the liquid amount sensor 32 detects it and repeats the above steps.
本実施形態の変形例を図６に示す。
本変形例に係る細胞培養装置３１０は、図６に示される構成の装置であり、培地供給手段４の管状部材に供給速度調整手段３３をさらに備え、液量センサ３２が供給速度調整手段３３による培養バッグ２への培地供給速度を調整することができる（培地供給速度を０にして供給を止めることもできる）装置である。
A modification of this embodiment is shown in FIG.
A cell culture apparatus 310 according to this modification is an apparatus having the configuration shown in FIG. 6, further including a supply speed adjustment means 33 on the tubular member of the medium supply means 4, and the liquid amount sensor 32 is based on the supply speed adjustment means 33. This is an apparatus capable of adjusting the medium supply rate to the culture bag 2 (the supply can be stopped by setting the medium supply rate to 0).
本変形例によれば、培地保持手段３内の培地が所定量に達すると液量センサ３２がそれを検知し、陰圧供給手段６および供給速度調整手段３３へ情報を送ることで陰圧供給手段６および供給速度調整手段３３を作動させ、培養バッグ２内からの培地の排出および培養バッグ２内への培地の供給を制御することができる。 According to this modification, when the culture medium in the culture medium holding means 3 reaches a predetermined amount, the liquid amount sensor 32 detects this and sends information to the negative pressure supply means 6 and the supply speed adjustment means 33 to supply negative pressure. The means 6 and the supply speed adjusting means 33 can be operated to control the discharge of the medium from the culture bag 2 and the supply of the medium to the culture bag 2.
次に、液量センサ３２による陰圧供給手段６および供給速度調整手段３３の制御の一例を示す。
液量センサ３２が作動する前は、陰圧供給手段６は停止した状態で、供給速度調整手段３３は培地の供給を止めた状態（流路を閉じ培地供給速度を０にした状態）となっている。培地保持手段３内の培地が所定量に達すると液量センサ３２がそれを検知し、陰圧供給手段６へ情報を送ることで陰圧供給手段６を作動させ、廃液保持手段５内を陰圧にする。このことにより、培養バッグ２内の培地が管状部材５ｃを介して廃液保持手段５内に吸引される。液量センサ３２は陰圧供給手段６へ情報を送ってから所定の時間間隔をおいてから供給速度調整手段３３へ情報を送り、流路を開いた状態にして培地の供給を開始する。陰圧供給手段６および供給速度調整手段３３は、液量センサ３２から情報を受けてから所定時間経過後に停止する様になっており、それらが停止すると培地保持手段３内の培地量が増加し始める。培地保持手段３内の培地が所定量に達すると液量センサ３２がそれを検知し、上記ステップを繰り返す。
このように、陰圧供給手段６および供給速度調整手段３３の作動のタイミングをずらすことにより、培地の交換効率をよくすることができる。
Next, an example of control of the negative pressure supply means 6 and the supply speed adjustment means 33 by the liquid amount sensor 32 will be shown.
Before the liquid amount sensor 32 is activated, the negative pressure supply means 6 is in a stopped state, and the supply speed adjusting means 33 is in a state where supply of the culture medium is stopped (a state where the flow path is closed and the culture medium supply speed is set to 0). ing. When the culture medium in the culture medium holding means 3 reaches a predetermined amount, the liquid amount sensor 32 detects it and sends information to the negative pressure supply means 6 to operate the negative pressure supply means 6, and the waste liquid holding means 5 is negatively charged. Pressure. Thereby, the culture medium in the culture bag 2 is sucked into the waste liquid holding means 5 through the tubular member 5c. The liquid amount sensor 32 sends information to the negative pressure supply means 6 and then sends information to the supply speed adjustment means 33 after a predetermined time interval, and starts supplying the culture medium with the flow path opened. The negative pressure supply means 6 and the supply speed adjustment means 33 stop after a predetermined time has elapsed since receiving information from the liquid amount sensor 32, and when they stop, the amount of medium in the medium holding means 3 increases. start. When the medium in the medium holding means 3 reaches a predetermined amount, the liquid amount sensor 32 detects it and repeats the above steps.
In this way, the medium exchange efficiency can be improved by shifting the operation timing of the negative pressure supply means 6 and the supply speed adjustment means 33.
ここで、液量センサ３２が陰圧供給手段６および供給速度調整手段３３に時間差をつけて情報を発信することにかえ、液量センサ３２が陰圧供給手段６および供給速度調整手段３３に同時に情報を発信し、供給速度調整手段３３が情報を受けてから所定時間経過後に作動する様にしても良い。 Here, instead of the liquid quantity sensor 32 transmitting information to the negative pressure supply means 6 and the supply speed adjustment means 33 with a time difference, the liquid quantity sensor 32 simultaneously transmits to the negative pressure supply means 6 and the supply speed adjustment means 33. Information may be transmitted, and the operation may be performed after a predetermined time has elapsed since the supply speed adjusting means 33 receives the information.
液量センサ３２、陰圧供給手段６、供給速度調整手段３３はタイマーを備えていることが好ましい。 The liquid amount sensor 32, the negative pressure supply means 6, and the supply speed adjustment means 33 preferably include a timer.
陰圧供給手段６および供給速度調整手段３３が作動開始してから所定時間経過後に停止することにかえ、液量センサ３２が培地保持手段３内の培地量が所定量まで減少した際にそれを検知し、陰圧供給手段６および供給速度調整手段３３へ情報を送ることで陰圧供給手段６および供給速度調整手段３３を停止させても良い。 Instead of the negative pressure supply means 6 and the supply speed adjusting means 33 being stopped after a lapse of a predetermined time from the start of operation, when the liquid amount sensor 32 reduces the amount of medium in the medium holding means 3 to a predetermined amount, The negative pressure supply means 6 and the supply speed adjustment means 33 may be stopped by detecting and sending information to the negative pressure supply means 6 and the supply speed adjustment means 33.
本実施形態および変形例において、送液ポンプ３１ｄを遠隔的に操作する制御部を備えていても良い。こうすることで、ユーザは、例えば図示しないモニタリングシステムで遠隔監視しながら、所望のタイミングで培地交換をすることができる。
また、あらかじめ設定したプログラムにより制御部が送液ポンプ３１ｄを自動制御しても良い。
In the present embodiment and the modification, a control unit that remotely operates the liquid feed pump 31d may be provided. By doing so, the user can change the medium at a desired timing while remotely monitoring with a monitoring system (not shown), for example.
Further, the control unit may automatically control the liquid feed pump 31d by a preset program.
なお、液量センサ３２から陰圧供給手段６や供給速度調整手段３３への情報の伝達は有線でも無線でも良い。 Information transmission from the liquid amount sensor 32 to the negative pressure supply means 6 and the supply speed adjustment means 33 may be wired or wireless.
本実施形態および変形例によれば、培地保存手段３１から培地保持手段３への培地供給速度を調整することで、所望の周期で培養バッグ内の培地の交換を行うことが可能となる。 According to the present embodiment and the modification, it is possible to exchange the medium in the culture bag at a desired cycle by adjusting the medium supply speed from the medium storage unit 31 to the medium holding unit 3.
上記各実施形態において、培地排出手段はインキュベータの内外いずれに設置しても良い。
また、陰圧供給手段６が廃液容器６２を備えている場合、廃液保持手段５を採用せず、廃液容器６２が廃液保持手段５の機能を有していても良い。この場合、廃液容器６２内に供給された培地が廃液容器６２内の空間を滴下される構成となっていることが好ましい。このことにより、培地の逆流を防ぐことができ、培養バッグ２内が汚染される（コンタミネーションが起こる）ことを防止することができる。
In each of the above embodiments, the medium discharging means may be installed either inside or outside the incubator.
Further, when the negative pressure supply means 6 includes the waste liquid container 62, the waste liquid holding means 5 may not be adopted, and the waste liquid container 62 may have the function of the waste liquid holding means 5. In this case, it is preferable that the medium supplied in the waste liquid container 62 is dropped in the space in the waste liquid container 62. Thereby, the back flow of the culture medium can be prevented, and the inside of the culture bag 2 can be prevented from being contaminated (contamination occurs).
上記各実施形態において、複数の培養バッグ２を使用する場合、培地保持手段３の排出口３ａからの管状部材４ａが分岐部４ｃで複数の管状部材４ｂに分岐し、それぞれが異なる培養バッグ２の供給口２ａに接続している態様を示したが、例えば図７に示すように、培地保持手段３が複数の排出口３ａを備え、それぞれが管状部材を介して異なる培養バッグ２の供給口２ａに接続している態様でも良い。
また、培養バッグは１つでも良い。
In each said embodiment, when using the some culture bag 2, the tubular member 4a from the discharge port 3a of the culture medium holding means 3 branches into the some tubular member 4b in the branch part 4c, and each is different culture bag 2 Although the aspect connected to the supply port 2a was shown, as shown, for example in FIG. 7, the culture medium holding means 3 is provided with a plurality of discharge ports 3a, and each supply port 2a of the culture bag 2 is different via a tubular member. The aspect connected to may be sufficient.
Moreover, one culture bag may be sufficient.
上記各実施形態において、陰圧供給手段６の操作は、ポンプ６１を作動・停止させることで陰圧の供給を制御しても良い。または、図８に示すように、廃液保持手段につながる管状部材に流路開閉ゲート８１を設置し、ポンプ６１を作動させた状態で、管状部材の流路を開閉することにより陰圧の供給を制御しても良い。
流路開閉ゲート８１の例としては、供給速度調整手段と同様であれば良く、図４に示す態様を例示できる。
また、流路開閉ゲート８１は制御部により遠隔的に制御されていても良い。遠隔的な制御は無線でも有線でも良い。
In each of the above embodiments, the operation of the negative pressure supply means 6 may control the supply of the negative pressure by operating and stopping the pump 61. Alternatively, as shown in FIG. 8, a negative pressure is supplied by opening and closing the flow path of the tubular member in a state where the flow path opening / closing gate 81 is installed in the tubular member connected to the waste liquid holding means and the pump 61 is operated. You may control.
An example of the flow path opening / closing gate 81 may be the same as that of the supply speed adjusting means, and the mode shown in FIG. 4 can be exemplified.
Further, the channel opening / closing gate 81 may be controlled remotely by the control unit. Remote control may be wireless or wired.
本発明の制御部としては、ＰＣを例示することができる。例えばＣＰＵとメモリを有するＰＣが、メモリに記憶された制御プログラムをＣＰＵが実行することで制御部としての機能を実現しても良い。 An example of the control unit of the present invention is a PC. For example, a PC having a CPU and a memory may realize a function as a control unit by the CPU executing a control program stored in the memory.
本発明に使用可能な培養バッグとしては、例えば図９に示したような内部に流路を有する培養バッグ９１を例示することができる。このように流路を形成することにより、培地の交換効率を上げることができる。培養バッグの対峙した面同士を一部癒着させることで培養バッグ内の空間に境界線９２をつくり、流路を形成しても良い。培養バッグ９１の開口部である供給口９３、排出口９４は、流路上で互いに離れた位置に設置すると培地に交換効率を上げることができる。また、排出口９４の口径（または断面面積）が、供給口９３の口径（または断面面積）より大きくても良い。 As a culture bag which can be used in the present invention, for example, a culture bag 91 having a channel inside as shown in FIG. 9 can be exemplified. By forming the flow path in this manner, the medium exchange efficiency can be increased. A boundary 92 may be formed in the space in the culture bag by partially bonding the facing surfaces of the culture bag to form a flow path. If the supply port 93 and the discharge port 94 which are openings of the culture bag 91 are installed at positions separated from each other on the flow path, the exchange efficiency of the culture medium can be increased. Further, the diameter (or cross-sectional area) of the discharge port 94 may be larger than the diameter (or cross-sectional area) of the supply port 93.
本発明において、供給速度調整手段１０、３３は、ペリスタルティックポンプ等の送液ポンプであっても良い。 In the present invention, the supply speed adjusting means 10, 33 may be a liquid feed pump such as a peristaltic pump.
１ インキュベータ
２ 培養バッグ
３ 培地保持手段
４ 培地供給手段
５ 培地排出手段
６ 陰圧供給手段
１０ 供給速度調整手段
３１ 培地保存手段
３１ａ 培地保存容器
３１ｂ 温度調節手段
３１ｃ 管状部材
３１ｄ 送液ポンプ
３２ 液量センサ
３３ 供給速度調整手段
４１ 開閉ゲート
６１ ポンプ
６２ 廃液容器
６３ 吸引口
８１ 流路開閉ゲート
９１ 培養バッグ
９２ しきい
９３ 供給口
９４ 排出口
DESCRIPTION OF SYMBOLS 1 Incubator 2 Culture bag 3 Medium holding means 4 Medium supply means 5 Medium discharge means 6 Negative pressure supply means 10 Supply speed adjustment means 31 Medium storage means 31a Medium storage container 31b Temperature adjustment means 31c Tubular member 31d Liquid feed pump 32 Liquid quantity sensor 33 Supply speed adjusting means 41 Open / close gate 61 Pump 62 Waste liquid container 63 Suction port 81 Channel open / close gate 91 Culture bag 92 Threshold 93 Supply port 94 Discharge port
Claims (6)
該培地保持手段と連結し、前記培地保持手段から排出された培地を培養バッグに供給する培地供給手段と、
該培地供給手段と接続する供給口と、内部から培地を排出するための排出口を備えた培養バッグと、
前記培養バッグの前記排出口に陰圧を供給する陰圧供給手段と、
前記培養バッグの前記排出口からの培地を保持する廃液保持手段を備えた細胞培養装置。 Medium holding means for holding a medium for culturing cells;
Medium supply means connected to the medium holding means and supplying the culture medium discharged from the medium holding means to the culture bag;
A culture bag having a supply port connected to the medium supply means, and a discharge port for discharging the medium from the inside;
Negative pressure supply means for supplying negative pressure to the outlet of the culture bag;
A cell culture apparatus comprising waste liquid holding means for holding a culture medium from the outlet of the culture bag.
前記培地保持手段内の培地の量を検出する液量センサと、をさらに備え、
前記培地保存手段から前記培地保持手段に供給された培地量が所定の量に達したときに、前記液量センサがそれを検出し、前記陰圧供給手段を作動させる請求項１に記載の細胞培養装置。 Medium storage means for maintaining the medium at a temperature suitable for storage and supplying the medium to the medium holding means;
A liquid amount sensor for detecting the amount of the medium in the medium holding means,
The cell according to claim 1, wherein when the amount of the medium supplied from the medium storage means to the medium holding means reaches a predetermined amount, the liquid amount sensor detects it and activates the negative pressure supply means. Culture device.
前記培地保持手段内の培地の量を検出する液量センサと、をさらに備え、
前記培地保存手段から前記培地保持手段に供給された培地量が所定の量に達したときに、前記液量センサがそれを検出し、前記陰圧供給手段および前記供給速度調整手段を作動させる請求項２に記載の細胞培養装置。 Medium storage means for maintaining the medium at a temperature suitable for storage and supplying the medium to the medium holding means;
A liquid amount sensor for detecting the amount of the medium in the medium holding means,
When the amount of culture medium supplied from the culture medium storage means to the culture medium holding means reaches a predetermined amount, the liquid amount sensor detects it and activates the negative pressure supply means and the supply speed adjustment means. Item 3. The cell culture device according to Item 2.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15/208,663 US20170022469A1 (en) | 2015-07-23 | 2016-07-13 | Cell culturing apparatus |
| EP16179936.6A EP3121259A1 (en) | 2015-07-23 | 2016-07-18 | Cell culturing apparatus |
| CN201610577281.6A CN106367342A (en) | 2015-07-23 | 2016-07-20 | Cell culturing apparatus |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015145830 | 2015-07-23 | ||
| JP2015145830 | 2015-07-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2017023131A true JP2017023131A (en) | 2017-02-02 |
Family
ID=57948867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016105714A Pending JP2017023131A (en) | 2015-07-23 | 2016-05-27 | Cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2017023131A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229752A (en) * | 2019-06-24 | 2019-09-13 | 上海长海医院 | A kind of tiny structure cell culture apparatus |
| WO2020003504A1 (en) * | 2018-06-29 | 2020-01-02 | 株式会社サンプラテック | Perfusion culture system |
| JP2021087364A (en) * | 2019-12-02 | 2021-06-10 | 株式会社日立製作所 | Cell culture apparatus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007020493A (en) * | 2005-07-19 | 2007-02-01 | Takagi Ind Co Ltd | Culture chamber, culturing apparatus and method for supplying culture solution |
| US20100316446A1 (en) * | 2005-01-04 | 2010-12-16 | Larry Runyon | Method and system for bioreaction |
| EP2623587A1 (en) * | 2012-02-03 | 2013-08-07 | Cellix Limited | Apparatus and method for performing experiments on live cells |
| WO2013114845A1 (en) * | 2012-02-01 | 2013-08-08 | 東洋製罐グループホールディングス株式会社 | Cell culture kit, and method of using cell culture kit |
| JP2014113109A (en) * | 2012-12-11 | 2014-06-26 | Kawasaki Heavy Ind Ltd | Medium exchange system and medium heating apparatus |
-
2016
- 2016-05-27 JP JP2016105714A patent/JP2017023131A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100316446A1 (en) * | 2005-01-04 | 2010-12-16 | Larry Runyon | Method and system for bioreaction |
| JP2007020493A (en) * | 2005-07-19 | 2007-02-01 | Takagi Ind Co Ltd | Culture chamber, culturing apparatus and method for supplying culture solution |
| WO2013114845A1 (en) * | 2012-02-01 | 2013-08-08 | 東洋製罐グループホールディングス株式会社 | Cell culture kit, and method of using cell culture kit |
| EP2623587A1 (en) * | 2012-02-03 | 2013-08-07 | Cellix Limited | Apparatus and method for performing experiments on live cells |
| JP2014113109A (en) * | 2012-12-11 | 2014-06-26 | Kawasaki Heavy Ind Ltd | Medium exchange system and medium heating apparatus |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020003504A1 (en) * | 2018-06-29 | 2020-01-02 | 株式会社サンプラテック | Perfusion culture system |
| JPWO2020003504A1 (en) * | 2018-06-29 | 2021-07-08 | 株式会社サンプラテック | Perfusion culture system |
| JP7162226B2 (en) | 2018-06-29 | 2022-10-28 | 株式会社サンプラテック | Perfusion culture system |
| CN110229752A (en) * | 2019-06-24 | 2019-09-13 | 上海长海医院 | A kind of tiny structure cell culture apparatus |
| CN110229752B (en) * | 2019-06-24 | 2024-03-19 | 上海长海医院 | Micro negative pressure cell culture device |
| JP2021087364A (en) * | 2019-12-02 | 2021-06-10 | 株式会社日立製作所 | Cell culture apparatus |
| JP7339138B2 (en) | 2019-12-02 | 2023-09-05 | 株式会社日立製作所 | Cell culture device |
| US11814614B2 (en) | 2019-12-02 | 2023-11-14 | Hitachi, Ltd. | Cell culture device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2016072460A1 (en) | Cell culture system and cell culture container | |
| EP3121259A1 (en) | Cell culturing apparatus | |
| JP2017023131A (en) | Cell culture apparatus | |
| US20150017711A1 (en) | Cell culture systems | |
| JP6675309B2 (en) | Cell culture system | |
| US20150344833A1 (en) | Medium exchange system | |
| US11478751B2 (en) | Automated single-use filtering apparatus, and method for controlling an automated single-use filtering apparatus | |
| US20190002815A1 (en) | Large-scale Bioreactor | |
| WO2019201270A1 (en) | Biological cartridge, biological cartridge assembly, microsphere preparation device, shell assembling device, biobrick preparation instrument, biological ink preparation instrument, and biological ink preparation system | |
| JP6062054B2 (en) | Liquid feeding device and cell culture device using the same | |
| WO2018042710A1 (en) | Liquid feed device, and cell culture device and method using same | |
| US20130000422A1 (en) | Collection unit | |
| WO2020018725A1 (en) | Automatized, programmable, high-throughput culture and analysis systems and methods | |
| US20170275579A1 (en) | Cell culture apparatus and cell culture bag | |
| KR102350723B1 (en) | Auto medium-changing system | |
| JP6134816B2 (en) | Cell culture equipment | |
| US20180320120A1 (en) | Cell culture container and culture-medium changing system | |
| JP2012026385A (en) | Liquid conveying device, liquid conveying kit | |
| JP2022119670A (en) | Cell culture apparatus | |
| JP7339138B2 (en) | Cell culture device | |
| JP2011145112A (en) | Mechanism and method of preventing suction air from leaking during filtration of capturing carrier solution | |
| JP2006141327A (en) | Culturing apparatus | |
| JP2008292186A (en) | Precise measuring system in batch plant | |
| JP2008086894A (en) | Liquid agent dropping device and defoaming method thereof | |
| JP2006141325A (en) | Culturing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20180309 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190524 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20200214 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200303 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20200923 |