CN218681326U

CN218681326U - Spider biotoxin collection system

Info

Publication number: CN218681326U
Application number: CN202222625595.2U
Authority: CN
Inventors: 赖仞; 容明强; 谷陟欣; 王文智; 温巧
Original assignee: Chengdu Peide Biomedical Co ltd
Current assignee: Chengdu Peide Biomedical Co ltd
Priority date: 2022-07-15
Filing date: 2022-09-30
Publication date: 2023-03-24
Anticipated expiration: 2032-09-30

Abstract

The utility model provides a spider biotoxin collecting device. The device comprises a negative pressure glove box and a toxin collecting mechanism, wherein the toxin collecting mechanism is arranged in the negative pressure glove box and comprises an electrical stimulator and a biotoxin collecting assembly, the biotoxin collecting assembly comprises a base and a plurality of collecting cups, the cup bodies of the collecting cups are cylindrical, and the cup mouths of the collecting cups are inverted truncated cones. The spider is stimulated by negative pressure and electric stimulation, so that the toxin can be more favorably removed, the organism of the spider can not be damaged, the spider can repeatedly collect the toxin after recovering for a period of time, and the repeated utilization rate of spider resources is effectively improved; the cup mouth of the collecting cup is used for isolating the chela and the mouthpart of the spider, so that the saliva secreted in the mouthpart is prevented from decomposing protein in the biotoxin, the bioactive components in the biotoxin are effectively ensured, and the quality of a biotoxin sample is improved. The device simple structure has reduced the risk that the staff is bitten by the spider, can realize gathering spider biotoxin in batches, has obvious economic benefits.

Description

Spider biotoxin collection system

Technical Field

The utility model relates to a biotoxin gathers technical field, concretely relates to spider biotoxin collection system.

Background

In recent years, it has been discovered that spider biotoxins are an important source of natural neurotoxins and are complex in composition, mainly polypeptides and proteins, including polypeptide neurotoxins, cytotoxins, enzymes, kinin analogs, aggregation-active peptides, antimicrobial peptides, and the like. In addition, the spider biotoxin contains polyamines, ATP, nucleotides, amino acids, low molecular weight substances such as single-limb substances, inorganic salts, and the like. Therefore, the spider biotoxin has high medical value and obvious economic benefit. The traditional method for collecting the toxin of the spider biotoxin is to catch the spider by hands or electrically stimulate the spider, and continuously irritate the spider, so that the spider bites the inner wall of a container and discharges the biotoxin. The toxin taking mode is easy to cause the danger that the human body is bitten by the spider, is easy to damage the body of the spider, and has low toxin taking efficiency, poor quality and small quantity of biotoxin.

How to obtain a large amount of spider biotoxins with controllable quality and ensure the safety of workers is a technical problem which needs to be solved urgently by the technical personnel in the field.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a spider biotoxin collecting device, which aims at the defects in the prior art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a spider biotoxin collection system, including negative pressure glove box and virus collection mechanism, be provided with on the negative pressure glove box and send appearance mouth and shift the mouth, virus collection mechanism sets up in the negative pressure glove box, virus collection mechanism includes electrical stimulation appearance and biotoxin collection component, biotoxin collection component includes base and a plurality of collection cup, the base sets up the bottom of negative pressure glove box, even interval is provided with a plurality of grooves of shelving on the base, it is in to collect the cup setting shelve the inslot, the cup body of collecting the cup is cylindricly, the rim of a cup of collecting the cup is the truncated cone of inversion.

Further, negative pressure glove box includes box and negative pressure generation system, negative pressure generation system sets up one side of box, the top of box is provided with at least one opening, the bottom of box is provided with send a kind mouth and shift the mouth, send a kind mouth drawably to install and send a kind box, but shift the mouth drawably to install and shift the box, send a kind box with the top that shifts the box is uncovered.

Furthermore, the sample sending box, the transfer box and the base are arranged at the bottom of the box body side by side, and the base is positioned between the sample sending box and the transfer box.

Furthermore, the inner walls of the sample sending box and the transfer box are both provided with butter coatings.

Further, send the appearance box with the transfer box all through installation draw runner cooperation installation spout with box sliding connection, first spout has all been seted up on the both sides inner wall of box, the second spout has all been seted up on the both sides outer wall of base, and relative first spout and the second spout that sets up constitute the installation spout, send the appearance box with all install on the both sides wall of transfer box the installation draw runner.

Furthermore, the electrical stimulation instrument comprises an adjustable power supply structure, a control panel and two electrodes, wherein the adjustable power supply structure is arranged inside the base, the electrodes are arranged on the upper surface of the base, and the control panel is arranged outside the base.

Furthermore, a plurality of spring pieces are arranged around the inner wall in the placing groove.

Further, the spider venom collecting device further comprises a movable support and an idler wheel installed below the movable support, and the movable support is arranged at the bottom of the negative pressure glove box.

Further, the box body is a transparent box body.

Furthermore, the gloves of the negative pressure glove box are breakage-proof latex gloves.

Compared with the prior art, the utility model provides a beneficial effect that technical scheme brought is:

(1) The utility model provides a pair of spider biotoxin collection system, including negative pressure glove box and toxin collection mechanism, toxin collection mechanism sets up in negative pressure glove box, and toxin collection mechanism includes electrical stimulation appearance and biotoxin collection component, and biotoxin collection component includes base and a plurality of collection cup, and the base setting is provided with the groove of shelving on the bottom of negative pressure glove box at even interval on the base, collects the cup setting and shelves the inslot, and the cup body of collecting the cup is cylindricly, the rim of a cup of collecting the cup is the truncated cone of inversion. By applying negative pressure stimulation and electrical stimulation to the spiders, the toxin expelling of the spiders is facilitated, the organism of the spiders is not damaged, and the spiders can repeatedly collect the toxins after recovering for a period of time, so that the repeated utilization rate of spider resources is greatly improved; the cup mouth of the collecting cup is in an inverted truncated cone shape and is used for isolating the chela and the mouthpart of the spider, the chela is arranged on the cup mouth, the mouthpart is arranged below the cup mouth, and when the spider secretes biotoxin through the chela after receiving electric stimulation, the biotoxin drops on the cup mouth, so that saliva secreted from the mouth after the spider receives electric stimulation is avoided, proteins in the biotoxin are decomposed, bioactive components in the biotoxin are effectively guaranteed, and the quality of a biotoxin sample is improved.

(2) The device of the utility model is simple in structure, reduced the risk that the staff is bitten by the spider, can realize gathering spider biotoxin in batches, single can accomplish whole virus collection process moreover, has improved virus collection efficiency, has obvious economic benefits.

Drawings

FIG. 1 is a schematic view of a spider biotoxin collecting device of the present invention;

figure 2 is the top view of the toxin collecting mechanism of the spider biotoxin collecting device of the utility model

Fig. 3 is a schematic diagram of the sample-sending box in the embodiment.

1. A negative pressure glove box; 11. a box body; 111. an opening; 112. a first chute; 113. a second chute; 12. a negative pressure generating system; 13. a sample sending box; 14. a transfer cassette; 15. installing a slide bar; 16. installing a chute; 152. installing a slide bar; 2. a poison collecting mechanism; 21. an electrical stimulator; 211. an adjustable power supply structure; 212. a control panel; 213. an electrode; 22. a biotoxin collection assembly; 221. a base; 222. a collection cup; 223. a resting groove; 224. a spring plate; 3. a sample feeding port; 4. a transfer port; 5. moving the support; 6. and a roller.

Detailed Description

The following are specific embodiments of the present invention and the accompanying drawings are used to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

As shown in fig. 1, the utility model discloses a spider biotoxin collection system, including negative pressure glove box 1 and toxin collection mechanism 2, be provided with on the negative pressure glove box 1 and send appearance mouth 3 and shift mouth 4, toxin collection mechanism 2 sets up in negative pressure glove box 1, toxin collection mechanism 2 includes electrical stimulation appearance 21 and biotoxin collection component 22, biotoxin collection component 22 includes base 221 and a plurality of collection cup 222, base 221 sets up the bottom at negative pressure glove box 1, even interval is provided with a plurality of grooves 223 of shelving on the base 221, collect cup 222 and set up in the groove 223 of shelving, the cup body of collecting cup 222 is cylindricly, the rim of a cup of collecting cup 222 is the truncated cone form of inversion, the thickness of rim of a cup is thin, can be 1 ~ 2mm. The electric stimulation is carried out on the spiders under negative pressure to collect the toxin, so that the toxin expelling of the spiders is facilitated, the body of the spiders is not damaged, and the spiders can repeatedly collect the toxin after recovering for a period of time, so that the repeated utilization rate of spider resources is greatly improved; the cup opening of the collecting cup is in an inverted truncated cone shape and is used for isolating a chela and a mouthpart of the spider, the chela is located on the cup opening, the mouthpart is located below the cup opening, and when the spider secretes biotoxin through the chela after receiving electric stimulation, the biotoxin drops on the cup opening, so that saliva secreted from the mouth after the spider receives the electric stimulation is avoided, proteins in the biotoxin are decomposed, bioactive components in the biotoxin are effectively guaranteed, and the quality of a biotoxin sample is improved. And, the device of the utility model is simple in structure, make things convenient for the staff to carry out batch collection spider biotoxin, reduced the risk that the staff was bitten by the spider simultaneously, single can accomplish whole toxin collection process moreover, the toxin collection efficiency of improvement has obvious economic benefits.

In some embodiments, in order to improve the virus collection efficiency and realize the virus collection of the batch spiders, as shown in fig. 2 and 3, the negative pressure glove box 1 may include a box body 11 and a negative pressure generating system 12, the negative pressure generating system 12 is disposed at one side of the box body 11, at least one opening 111 is disposed above the box body 11, a sample feeding port 3 and a transfer port 4 are disposed at the bottom of the box body 11, the sample feeding port 3 is installed with a sample feeding box 13 in a drawable manner, and the transfer port 4 is installed with a transfer box 14 in a drawable manner.

In some embodiments, in order to facilitate the operation of the worker, the sample feeding cassette 13, the transfer cassette 14, and the base 221 may be arranged side by side at the bottom of the case 11, the base 221 may be located between the sample feeding cassette 13 and the transfer cassette 14, the sample feeding cassette 13 may be used to feed spiders to be sampled to the negative pressure generating system 12, and after the worker finishes sampling the toxins, the spiders may be put into the transfer cassette 14 and taken out to be put back into the culture chamber for culture.

In some embodiments, to prevent spiders from escaping, the inner walls of the sample transport box 13 and the transfer box 14 may each be provided with a butter coating.

In some embodiments, in order to realize batch collection of spider toxins and facilitate operation of workers, the sample feeding box 13 and the transfer box 14 can be both connected with the box body 11 in a sliding manner through the installation sliding strips 15 in a matching manner, the inner walls of two sides of the box body 11 are both provided with the first sliding grooves 112, the outer walls of two sides of the base 221 are both provided with the second sliding grooves 113, the first sliding grooves 112 and the second sliding grooves 113 which are arranged oppositely form the installation sliding grooves 16, and the two side walls of the sample feeding box 13 and the two side walls of the transfer box 14 are both provided with the installation sliding strips 15.

In some embodiments, in order to make the space layout of the drug administration facility more reasonable, the electrical stimulator 21 may include an adjustable power supply structure 211, a control panel 212, and two electrodes 213, wherein the adjustable power supply structure 211 is disposed inside the base 221, the electrodes 213 are disposed on the upper surface of the base 221, and the control panel 212 is disposed outside the base 221.

In some embodiments, a plurality of spring tabs 224 are provided around the inner wall within the resting groove 223 for better stability of the collection cup.

In some embodiments, for the convenience of use and transfer of the spider venom device, a movable bracket 5 and a roller 6 installed at the bottom of the movable bracket 5 may be further included, and the movable bracket 5 is disposed at the bottom of the negative pressure glove box 1.

In some embodiments, the housing may be a transparent housing in order to allow for viewing and prevent virus errors.

In some embodiments, the gloves of the negative pressure glove box 1 may be breakage-proof latex gloves in order to facilitate the ability of the worker to better grip the spider and not get bitten.

The utility model discloses a process that spider biotoxin collection system realized biotoxin collection does: the spider enters the negative pressure glove box 1 through the sample conveying box 13, the vacuum pressure of the negative pressure glove box 1 is set to be gradually increased from the range of 0.00-0.50 MPa, after 5-10 min, an opening in the top of the box body 11 is opened, a worker carries a breakage-proof latex glove, the spider is fixed on the collection cup 222 through the left thumb and the index finger, the cup mouth of the collection cup 222 is inserted below the spider chela, the upper side of the mouthpart is arranged above the mouthpart, the touch limb of the spider tightly holds the cup body of the collection cup 222, the right hand respectively contacts the two electrodes 213 of the electrical stimulator 21 with the tail of the spider and the root of the spider chela, the output intensity and the frequency of the electrical stimulator 21 are adjusted, namely, the biotoxin is discharged and is stained on the upper surface of the cup mouth of the collection cup 222, if saliva secretion exists in the mouthpart, the mouth of the collection cup 222 is stained below the cup mouth, the electrical stimulator can be wiped off by using alcohol cotton swabs and dry cotton in sequence, and the spider after being collected is transferred to a culture room through the transfer box 14 to be cultured. The single spider of independently accomplishing adopts the poison work, can also realize single batch and adopt the poison, the utility model discloses a spider adopts the poison device not only had guaranteed the quality of spider biotoxin, has also improved and has adopted the poison work efficiency, prevents simultaneously that the staff from being bitten by the spider.

The above is not relevant and is applicable to the prior art.

While certain specific embodiments of the present invention have been described in detail by way of illustration, it should be understood by those skilled in the art that the foregoing examples are for purposes of illustration only and not limitation, as various modifications or additions may be made to the described specific embodiments or alternatives thereto, as will be apparent to those skilled in the art, without departing from the scope of the invention or exceeding the scope of the claims. It should be understood by those skilled in the art that any modifications, equivalent substitutions, improvements and the like made to the above embodiments according to the technical spirit of the present invention should be included in the scope of the present invention.

Claims

1. A spider biotoxin collection system, characterized in that: comprises a negative pressure glove box (1) and a poison collecting mechanism (2), wherein a sample feeding port (3) and a transfer port (4) are arranged on the negative pressure glove box (1),

the toxin collecting mechanism (2) is arranged in the negative pressure glove box (1), the toxin collecting mechanism (2) comprises an electric stimulator (21) and a biotoxin collecting assembly (22), the biotoxin collecting assembly (22) comprises a base (221) and a plurality of collecting cups (222), the base (221) is arranged at the bottom of the negative pressure glove box (1), a plurality of placing grooves (223) are uniformly arranged on the base (221) at intervals, the collecting cups (222) are arranged in the placing grooves (223), cup bodies of the collecting cups (222) are cylindrical, and cup mouths of the collecting cups (222) are inverted truncated cones.

2. The spider biotoxin collecting device according to claim 1, wherein the negative pressure glove box (1) comprises a box body (11) and a negative pressure generating system (12), the negative pressure generating system (12) is disposed on one side of the box body (11), the top of the box body (11) is provided with at least one opening (111), the bottom of the box body (11) is provided with the sample sending port (3) and the transfer port (4), the sample sending port (3) is provided with a sample sending box (13) in a drawable manner, the transfer port (4) is provided with a transfer box (14) in a drawable manner, and the tops of the sample sending box (13) and the transfer box (14) are open.

3. The aragonitoxin collection device according to claim 2, wherein said sample presentation cassette (13), said transfer cassette (14), and said base (221) are disposed side by side at the bottom of said housing (11), said base (221) being located between said sample presentation cassette (13) and said transfer cassette (14).

4. The spider biotoxin collection device of claim 3, wherein the sample transport cassette (13) and the transfer cassette (14) are each coated with a butter coating on their inner walls.

5. The spider biotoxin collecting device as claimed in claim 4, wherein the sample feeding box (13) and the transfer box (14) are slidably connected to the box body (11) through a mounting slide (15) and a mounting slide (16), the inner walls of the two sides of the box body (11) are respectively provided with a first slide (112), the outer walls of the two sides of the base (221) are respectively provided with a second slide (113), the mounting slide (16) is formed by the first slide (112) and the second slide (113) which are oppositely arranged, and the mounting slides (15) are respectively mounted on the two side walls of the sample feeding box (13) and the transfer box (14).

6. The aragonitoxin collection device according to claim 1, wherein said electrical stimulator (21) comprises an adjustable power supply structure (211), a control panel (212) and two electrodes (213), said adjustable power supply structure (211) is disposed inside said base (221), said electrodes (213) are disposed on the upper surface of said base (221), and said control panel (212) is disposed outside said base (221).

7. The spider bio-toxin collection device according to claim 1, wherein a plurality of spring pieces (224) are provided around an inner wall in the resting groove (223).

8. The spider biotoxin collecting device according to claim 3, further comprising a moving bracket (5) and rollers (6) mounted on the bottom of the moving bracket (5), wherein the moving bracket (5) is disposed on the bottom of the negative pressure glove box (1).

9. The spider biotoxin collection device of claim 3, wherein the housing (11) is a transparent housing.

10. The spider biotoxin collecting device as defined in claim 1, wherein the gloves of the negative pressure glove box (1) are breakage-proof latex gloves.