CN219915074U - Full-automatic chromosome slide pelleter - Google Patents
Full-automatic chromosome slide pelleter Download PDFInfo
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- CN219915074U CN219915074U CN202321531655.2U CN202321531655U CN219915074U CN 219915074 U CN219915074 U CN 219915074U CN 202321531655 U CN202321531655 U CN 202321531655U CN 219915074 U CN219915074 U CN 219915074U
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- axis motor
- axis
- motor
- code scanning
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- 210000000349 chromosome Anatomy 0.000 title claims abstract description 20
- 238000007664 blowing Methods 0.000 claims abstract description 18
- 238000005507 spraying Methods 0.000 claims abstract description 18
- 239000011521 glass Substances 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 230000002572 peristaltic effect Effects 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 8
- 239000006285 cell suspension Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000007605 air drying Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000002559 cytogenic effect Effects 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000008570 general process Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a full-automatic chromosome slide making machine, which belongs to the field of sample pretreatment and comprises a control device, a drying device, a base and a frame arranged on the base, wherein a Y axis, an X axis and a Z axis which are overlapped are arranged on the frame, a glass carrying table and a sample test tube placing table are arranged on the base, a Y axis motor is arranged on the Y axis, an X axis motor is arranged on the X axis, and a Z axis motor, a liquid transferring pump, a instilling device, a spraying device and a blowing device are arranged on the Z axis. The chromosome sample slide of the slide making machine is quick to manufacture, labor-saving and simple to operate.
Description
Technical Field
The utility model relates to a full-automatic chromosome slide pelleter, and belongs to the field of sample pretreatment.
Background
In cytogenetic studies, in order to spread the chromosomes apart from each other and clearly show a constriction, it is often necessary to subject the dividing or already harvested cells to a series of pre-treatments, the purpose of which is to make the state of the chromosomes more suitable for analysis and study. 72 hours after culturing the cells (PHA is added to the culture medium), the cells synthesizing DNA account for 45% of the total number, the rate of mitosis corresponds to 1% per hour, and activated lymphocytes account for 90% of the total number, which is the best timing for harvesting cells in the division stage and preparing chromosome specimens. The main links from harvesting to tabletting are spindle inhibitor, hypotonic treatment, fixing, dripping tablet and the like.
The fixing step is followed by a tabletting stage. In the early days, people mainly adopted extrusion methods, and the air drying method was quickly replaced. Today, the air drying method is widely applied in cytogenetics of mammals and human beings, and the extrusion method has been forgotten almost.
The air dry method is mainly applied to the tissues such as bone marrow, peripheral lymphocyte, ascites, germ epithelial cells and the like which are easy to prepare cell suspension. The general process is that tissue blocks are grown into monolayer cells by in vitro culture technology, and then are subjected to enzymolysis into cell suspension. Once the cells are in suspension, they can be pretreated and fixed and then air dried to form a tablet.
The immobilized cells are centrifuged through a suspension, most of the supernatant is removed, a large amount of cells are suspended in a small amount of the immobilized solution at the bottom of the tube, and the clean glass slide is placed in cold double distilled water or pure alcohol. When in use, the cleaned forceps are used for clamping the glass slide on the backing plate, and the glass slide forms a certain angle (10-15 degrees) with the backing plate. Several drops of the cell suspension were pipetted onto the slide. The cell suspension thus dropped can be spread down the surface of the wet slide up and down. On one slide, approximately 2/3 of the area can hold all cells in 0.2 ml of whole blood culture. Immediately after the dripping, the backing plate and the slide are put into a 75 ℃ oven together, and the slide is baked for 3 hours to volatilize the fixing liquid.
The above embodiments are all realized manually, so that the labor cost is increased and the efficiency is low.
Disclosure of Invention
In view of the above, the present utility model provides a fully automatic chromosome slide pelleter to solve the above-mentioned problems in the prior art.
The technical scheme for solving the technical problems is as follows:
the full-automatic chromosome slide making machine comprises a control device, a drying device, a base and a frame arranged on the base, wherein the frame is arranged at four corners of the top surface of the base, Y shafts, X shafts and Z shafts which are overlapped are arranged on the frame, and the Y shafts comprise two shafts which are arranged in parallel;
the base is provided with a glass carrying table and a sample test tube placing table; the Y-axis is provided with a Y-axis motor; an X-axis motor is arranged on the X-axis; the Z axis is provided with a Z axis motor, a liquid transferring pump, a instilling device, a spraying device and a blowing device; the drying device, the Y-axis motor, the X-axis motor, the Z-axis motor, the liquid transferring pump, the instilling device, the spraying device and the blowing device are respectively and electrically connected with the control device; and an air outlet of the drying device corresponds to the position of the glass carrying table.
On the basis of the technical scheme, the utility model can be improved as follows.
Further, the Y-axis motor comprises a first Y-axis motor and a second Y-axis motor which are respectively arranged on the two shafts.
Further, the Z-axis motor comprises a first Z-axis motor and a second Z-axis motor, the spraying device and the blowing device are arranged on the first Z-axis motor, and the liquid transferring pump is arranged on the second Z-axis motor.
Further, the code scanning device comprises a code scanning module, wherein the code scanning module comprises a first code scanning gun arranged on the base, a code scanning motor in transmission connection with the first code scanning gun and a second code scanning gun arranged on the Z axis, and the code scanning module is electrically connected with the control device.
Further, the bottom of the glass carrying table is provided with a vibration motor.
Further, the spraying device comprises a spraying motor and a spraying peristaltic pump; the blowing device comprises a blowing motor.
The beneficial effects of the utility model are as follows:
1. chromosome sample slide preparation is fast: at the end of one round of workflow, 64 chromosome slides may be made.
2. Labor is saved: after the pelleter is opened, the staff only needs to put the sample test tube that is processed into pelleter appointed test tube and place the position, closes the bin gate, and pelleter alright be according to predetermineeing parameter accurate operation, only need a staff alright accomplish loaded down with trivial details pelleter work, the loss of staff physical power, energy that significantly reduces.
3. The operation is simple: the operator only needs to put the sample tube into the sample tube placing table and place the slide on the glass carrying table, and the control system can manufacture the sample slide according to the parameters set by the operator by setting the interface parameters.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model.
In the drawings, the list of components represented by the various numbers is as follows:
1. x-axis motor, 2, first Y-axis motor, 3, second Y-axis motor, 4, first Z-axis motor, 5, second Z-axis motor, 6, first yard rifle that sweeps, 7, second sweep yard rifle, 8, sweep yard motor, 9, glass-carrying platform, 10, pipetting pump, 11, sample test tube placement platform, 12, atomizer, 13, blowing device.
Detailed Description
The principles and features of the present utility model are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the utility model and are not to be construed as limiting the scope of the utility model.
As shown in fig. 1, the full-automatic chromosome slide pelleter comprises a control device, a drying device, a base and a rack arranged on the base, wherein the rack is arranged at four corners of the top surface of the base, and is provided with a Y axis, an X axis and a Z axis which are overlapped, and the Y axis comprises two axes which are arranged in parallel;
the base is provided with a glass carrying table 9 and a sample test tube placing table 11; a Y-axis motor is arranged on the Y-axis; an X-axis motor 1 is arranged on the X-axis; the Z axis is provided with a Z axis motor, a liquid transferring pump 10, a instilling device, a spraying device 12 and a blowing device 13; the drying device, the Y-axis motor, the X-axis motor 1, the Z-axis motor, the liquid transferring pump 10, the instilling device, the spraying device 12 and the blowing device 13 are respectively and electrically connected with the control device; the air outlet of the drying device corresponds to the position of the glass carrying table 9.
In another embodiment of the present utility model, the Y-axis motor includes a first Y-axis motor 2 and a second Y-axis motor 3 respectively provided on two axes.
In another embodiment of the utility model, the Z-axis motor comprises a first Z-axis motor 4 and a second Z-axis motor 5, the spraying device 12 and the blowing device 13 are arranged on the first Z-axis motor 4, and the pipetting pump 10 is arranged on the second Z-axis motor 5.
In another embodiment of the utility model, the code scanning device further comprises a code scanning module, wherein the code scanning module comprises a first code scanning gun 6 arranged on the base, a code scanning motor 8 in transmission connection with the first code scanning gun 6 and a second code scanning gun 7 arranged on the Z axis, and the code scanning module is electrically connected with the control device.
In another embodiment of the present utility model, a vibration motor is provided at the bottom of the glass carrier 9.
In another embodiment of the utility model, the spraying device 12 comprises a spraying motor, a spraying peristaltic pump; the blowing device 13 includes a blowing motor.
In another embodiment of the utility model, a shell with a side provided with a normally closed cabinet door is arranged outside the full-automatic chromosome slide pelleter.
The X-axis motor 1, the first Y-axis motor 2 and the second Y-axis motor 3 are driven to precisely move in the XY plane coordinates to find coordinate points of all positions, the code scanning motor 8 and the second code scanning gun 7 are driven to complete matching of sample slides, and the second Z-axis motor 5 and the liquid transferring pump 10 are further driven to complete extraction and instillation of samples to the slides; the first Z-axis motor 4 is driven to complete wetting of the slide with the spraying device 12 and the blowing device 13, and the slide is further dried by using a drying system.
The operator only needs to put a test tube containing a sample (such as a blood sample) into the sample tube placing table 11, further place a slide on the glass carrying table 9, and by setting interface parameters, the control system can make the sample slide according to the parameters set by the operator.
The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the utility model are intended to be included within the scope of the utility model.
Claims (6)
1. The full-automatic chromosome slide making machine is characterized by comprising a control device, a drying device, a base and a frame arranged on the base, wherein the frame is arranged at four corners of the top surface of the base, Y shafts, X shafts and Z shafts which are overlapped are arranged on the frame, and the Y shafts comprise two shafts which are arranged in parallel;
the base is provided with a glass carrying table and a sample test tube placing table; the Y-axis is provided with a Y-axis motor; an X-axis motor is arranged on the X-axis; the Z axis is provided with a Z axis motor, a liquid transferring pump, a instilling device, a spraying device and a blowing device; the drying device, the Y-axis motor, the X-axis motor, the Z-axis motor, the liquid transferring pump, the instilling device, the spraying device and the blowing device are respectively and electrically connected with the control device; and an air outlet of the drying device corresponds to the position of the glass carrying table.
2. The fully automated chromosome slide processing machine of claim 1, wherein the Y-axis motor comprises a first Y-axis motor and a second Y-axis motor disposed on two axes, respectively.
3. The fully automated chromosome slide processing machine of claim 1, wherein the Z-axis motor comprises a first Z-axis motor and a second Z-axis motor, the spraying device and the air blowing device are disposed on the first Z-axis motor, and the transfer pump is disposed on the second Z-axis motor.
4. The fully automatic chromosome slide pelleter of claim 1 further comprising a code scanning module comprising a first code scanning gun disposed on the base, a code scanning motor in driving connection with the first code scanning gun, and a second code scanning gun disposed on the Z-axis, the code scanning module being electrically connected with the control device.
5. The fully automatic chromosome slide pelleter of claim 1 wherein the glass carrier bottom is provided with a vibration motor.
6. The fully automatic chromosome slide pelleter of claim 1 wherein the spraying device comprises a spray motor, a spray peristaltic pump; the blowing device comprises a blowing motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321531655.2U CN219915074U (en) | 2023-06-15 | 2023-06-15 | Full-automatic chromosome slide pelleter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321531655.2U CN219915074U (en) | 2023-06-15 | 2023-06-15 | Full-automatic chromosome slide pelleter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219915074U true CN219915074U (en) | 2023-10-27 |
Family
ID=88437626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321531655.2U Active CN219915074U (en) | 2023-06-15 | 2023-06-15 | Full-automatic chromosome slide pelleter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219915074U (en) |
-
2023
- 2023-06-15 CN CN202321531655.2U patent/CN219915074U/en active Active
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