CN218666018U - Cell culture device for tumor immunotherapy - Google Patents

Abstract

The utility model discloses a cell culture device for tumour immunotherapy relates to medical instrument technical field, specifically is a cell culture device for tumour immunotherapy, including the artificial containers, the culture dish has been placed to the inner chamber of artificial containers, the fixed cover in inner chamber of artificial containers is equipped with fixed damping cover, the fixed cover in the outside of artificial containers is equipped with down the vacuum cover, the top of artificial containers is provided with the capping lid, the fixed cover in inboard of capping lid is equipped with movable damping cover. The fixed suit vacuum cover down in the outside that makes the artificial containers, fix the suit vacuum cover in the outside of closing cap simultaneously, utilize the cooperation of vacuum cover and last vacuum cover down, can restrict the ambient temperature of artificial containers and closing cap inner chamber, can avoid inside temperature to receive ambient temperature's influence, the setting of fixed damping cover and movable damping cover simultaneously, under the cooperation of friction line, can conveniently guarantee sealed effect, it is very fast to avoid inside and outside air interactive speed.

Description

Cell culture device for tumor immunotherapy

Technical Field

The utility model relates to the technical field of medical equipment, specifically a cell culture device for tumour immunotherapy.

Background

Normally, the immune system can recognize and eliminate tumor cells in the tumor microenvironment, but for survival and growth, tumor cells can adopt different strategies, so that the human immune system is inhibited and can not normally kill tumor cells, thereby being survived at each stage of the anti-tumor immune response, the above characteristic of tumor cells is called immune escape, and in order to better understand the multi-stage and multi-stage complexity of tumor immunity, the concept of tumor-immune cycle is developed and proposed.

In the process of culturing tumor immunotherapy cells, culture dishes are used for culturing, and issued patents, patent numbers: CN213012843U cell culture dish, its technical means who publishes: divide into interior chamber and outer chamber with the culture dish through detachable separator, because cell membrane requires the cell density in the unit area great, so when the cultured cell, add the culture solution in the interior chamber of culture dish, inoculate interior chamber with the cell according to the density of cell in the cell membrane, can guarantee the density of cell on the cell membrane piece, after the cell adherence, the interior culture solution of suction inner chamber, take out the separator from the culture dish, make the cell in the cell membrane all expose the outer chamber at the culture dish, add sufficient culture solution at outer chamber and provide the nutrient for the cell, its technical means has realized: the biological function of the cell membrane is detected in vitro, the stability of the nutrition supply of the cells can be ensured, but in the process of culturing the cells, except for controlling the culture solution, the culture environment also plays a crucial role in the growth of the cells, the growth environment of the cells has strict requirements on temperature and needs to be controlled at the proper temperature of a human body, the proper temperature of the general cells cultured in vitro is 37-38 ℃, and if the culture temperature is not controlled, the survival efficiency of the cell culture is difficult to accurately control, so that a cell culture device for tumor immunotherapy is provided.

SUMMERY OF THE UTILITY MODEL

To the not enough of prior art, the utility model provides a cell culture device for tumour immunotherapy has solved the problem that proposes in the above-mentioned background art.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a cell culture device for tumour immunotherapy, includes the artificial containers, the culture dish has been placed to the inner chamber of artificial containers, the fixed cover in inner chamber of artificial containers is equipped with fixed damping cover, the fixed cover in the outside of artificial containers is equipped with down the vacuum cover, the top of artificial containers is provided with the capping lid, the fixed cover in inboard of capping lid is equipped with movable damping cover, the fixed cover in the outside of capping lid is equipped with the vacuum cover, the fixed cover in the inside of capping lid and last vacuum cover is equipped with the air supply pipe, the fixed cover in inside of going up vacuum cover and capping lid is equipped with the blast pipe.

Optionally, a support ring is fixed to the inner cavity of the exhaust pipe, a plugging ring is sleeved on the inner cavity of the exhaust pipe, a threaded column is sleeved on the inner cavity of the support ring, a connection disc is movably connected to the bottom end of the threaded column, a connection spring is fixedly mounted on the bottom surface of the connection disc, a plugging disc is fixedly connected to the bottom end of the connection spring, and a knob is fixedly mounted at the top end of the threaded column.

Optionally, the top surfaces of the lower vacuum sleeve and the incubator and the fixed damping sleeve are located on the same horizontal plane, and the bottom surfaces of the upper vacuum sleeve and the top sealing cover are located on the same horizontal plane.

Optionally, friction grains are arranged on the outer side of the fixed damping sleeve and the inner side of the movable damping sleeve, and the bottom end of the movable damping sleeve is movably sleeved between the outer side of the fixed damping sleeve and the inner side of the incubator.

Optionally, the bottom ends of the air supply pipe and the exhaust pipe extend downwards to the lower part of the top surface of the inner cavity of the top cover, and the bottom ends of the air supply pipe and the exhaust pipe are located above the bottom surfaces of the top cover and the upper vacuum sleeve.

Optionally, the knob is located above the exhaust pipe, and the axes of the threaded column, the connecting disc, the plugging disc and the exhaust pipe are located on the same vertical line.

Optionally, the bottom surface of the plugging disc is attached to the top surface of the plugging ring, and the diameter value of the plugging disc is larger than the inner diameter value of the plugging ring.

The utility model provides a cell culture device for tumour immunotherapy possesses following beneficial effect:

1. this a cell culture device for tumour immunotherapy, setting through artificial containers and capping lid, vacuum cover under the fixed suit in the outside that makes artificial containers, vacuum cover is gone up to the fixed suit in the outside of capping lid simultaneously, the cooperation of vacuum cover and last vacuum cover under the utilization, can restrict the ambient temperature of artificial containers and capping inner chamber, can avoid inside temperature to receive ambient temperature's influence, the setting of fixed damping cover and movable damping cover simultaneously, under the cooperation of friction line, can conveniently guarantee sealed effect, it is very fast to avoid inside and outside air interaction speed.

2. This a cell culture device for tumour immunotherapy, through fixed suit has plenum pipe and blast pipe in the top of closing cap, utilizes the setting of the shutoff ring of blast pipe and shutoff dish, under the cooperation of connecting spring and connection pad, can restrict connecting spring's pressure, the cooperation of plenum pipe and blast pipe, the inner chamber temperature that can control the artificial containers is in suitable breed ambient temperature, conveniently improves the efficiency of cultivateing.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the split structure of the present invention;

FIG. 3 is a schematic structural view of the capping cover of the present invention;

FIG. 4 is a schematic structural view of the incubator of the present invention;

fig. 5 is a schematic structural view of the exhaust pipe of the present invention.

In the figure: 1. an incubator; 2. fixing a damping sleeve; 3. rubbing the lines; 4. a culture dish; 5. a lower vacuum sleeve; 6. a capping cover; 7. a movable damping sleeve; 8. putting a vacuum sleeve; 9. an air supply pipe; 10. an exhaust pipe; 11. a support ring; 12. a threaded post; 13. a knob; 14. a connecting disc; 15. a connecting spring; 16. a plugging disc; 17. and (6) plugging the ring.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1 to 4, the present invention provides a technical solution: a cell culture device for tumor immunotherapy comprises an incubator 1, a culture dish 4 is placed in an inner cavity of the incubator 1, a fixed damping sleeve 2 is fixedly sleeved on the inner cavity of the incubator 1, a lower vacuum sleeve 5 is fixedly sleeved on the outer side of the incubator 1, the upper surfaces of the lower vacuum sleeve 5, the incubator 1 and the fixed damping sleeve 2 are located on the same horizontal plane, the bottom surfaces of an upper vacuum sleeve 8 and a top sealing cover 6 are located on the same horizontal plane, the incubator 1 and the top sealing cover 6 are arranged to play a role in sealing the culture dish 4, the lower vacuum sleeve 5 fixedly sleeved on the outer side of the incubator 1 and the upper vacuum sleeve 8 fixedly sleeved on the outer side of the top sealing cover 6 play a role in insulating temperature when in use, the temperature of the inner cavity of the incubator 1 and the temperature of the inner cavity of the top sealing cover 6 can be prevented from changing fast with the external temperature, and the stability of cell growth can be effectively prevented from being influenced, a top sealing cover 6 is arranged above the incubator 1, a movable damping sleeve 7 is fixedly sleeved on the inner side of the top sealing cover 6, friction grains 3 are arranged on the outer side of the fixed damping sleeve 2 and the inner side of the movable damping sleeve 7, the bottom end of the movable damping sleeve 7 is movably sleeved between the outer side of the fixed damping sleeve 2 and the inner side of the incubator 1, the culture dish 4 is placed in the inner cavity of the incubator 1, after the placement is finished, an upper vacuum sleeve 8 is covered downwards from the upper part of a lower vacuum sleeve 5, the movable damping sleeve 7 is inserted to the outer side of the fixed damping sleeve 2 from the space between the fixed damping sleeve 2 and the incubator 1, the bottom surface of the top sealing cover 6 is attached to the top surface of the incubator 1, the bottom end of the movable damping sleeve 7 is inserted between the incubator 1 and the fixed damping sleeve 2, an upper vacuum sleeve 8 is fixedly sleeved on the outer side of the top sealing cover 6, an air supply pipe 9 is fixedly sleeved inside the top sealing cover 6 and the upper vacuum sleeve 8, go up the fixed cover in inside of vacuum sleeve 8 and capping 6 and be equipped with blast pipe 10, utilize the inboard friction line 3 of movable damping cover 7 and the outside friction line 3's of fixed damping cover 2 cooperation, play sealed effect when using, can avoid separating easily between artificial containers 1 and the capping 6, make things convenient for device's use, improved the practicality of device.

Referring to fig. 5, a support ring 11 is fixed in the inner cavity of the exhaust pipe 10, the bottom ends of the air supply pipe 9 and the exhaust pipe 10 extend downward to below the top surface of the inner cavity of the top cover 6, the bottom ends of the air supply pipe 9 and the exhaust pipe 10 are located above the bottom surfaces of the top cover 6 and the upper vacuum jacket 8, the air supply pipe 9 is arranged to supply air, the exhaust pipe 10 is arranged to exhaust air, when in use, the gas with the proper temperature in the inner cavities of the incubator 1 and the capping cover 6 can be fed through the gas feed pipe 9 to control the external environment of the culture dish 4, so that the culture dish 4 can be used at the proper temperature, the inner cavity of the exhaust pipe 10 is fixedly sleeved with the plugging ring 17, the inner cavity of the support ring 11 is fixedly sleeved with the threaded column 12, the rotating button 13 is positioned above the exhaust pipe 10, the threaded column 12, the connecting disc 14, the axis of the plugging disc 16 and the axis of the exhaust pipe 10 are positioned on the same vertical line, the arrangement of the threaded column 12 and the support ring 11 plays a role in controlling the pressure of the connecting spring 15, thereby limiting the exhaust speed of the gas in the inner cavity of the incubator 1, simultaneously controlling the external air pressure of the culture dish 4, improving the culture efficiency of the culture dish 4, facilitating the use of the device, the bottom end of the threaded column 12 is movably connected with a connecting disc 14, the bottom surface of the connecting disc 14 is fixedly provided with a connecting spring 15, the bottom end of the connecting spring 15 is fixedly connected with a plugging disc 16, the top end of the threaded column 12 is fixedly provided with a rotating button 13, by rotating the knob 13, the knob 13 drives the threaded column 12 to rotate, so that the threaded column 12 rotates in the inner cavity of the support ring 11, the position of the threaded column 12 is controlled, so that the threaded column 12 controls the connecting disc 14 to move to a proper position, and then the air pressure of the inner cavity incubator of the incubator 1 can be controlled by controlling the pressure applied to the connecting spring 15 by using the position of the connecting disc 14.

In summary, when the cell culture device for tumor immunotherapy is used, firstly, culture solution and cells are placed in an inner cavity of a culture dish 4, then the culture dish 4 is placed in an inner cavity of an incubator 1, after the culture dish is placed, an upper vacuum sleeve 8 is covered downwards from the upper part of a lower vacuum sleeve 5, a movable damping sleeve 7 is inserted to the outer side of the fixed damping sleeve 2 from the space between the fixed damping sleeve 2 and the incubator 1, the bottom surface of a capping cover 6 is attached to the top surface of the incubator 1, the bottom end of the movable damping sleeve 7 is inserted between the incubator 1 and the fixed damping sleeve 2, dry gas is fed into an inner cavity of a heat pipe 9 from the upper part of an air feed pipe 9 in the process of incubation by using the arrangement of friction lines 3 on the outer side of the fixed damping sleeve 2 and the inner side of the movable damping sleeve 7, the gas enters the inner cavity of the incubator 1 and the capping cover 6, the air feed pipe 1 is controlled to control the inner cavity of the incubator 1 and the capping sleeve 6, the air feed pipe 6 is pushed out from the upper sealing ring 16, and the air feed pipe 8, and the upper sealing ring 16 are separated from the upper sealing ring 16.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. A cell culture device for tumour immunotherapy, includes incubator (1), culture dish (4) have been placed to the inner chamber of incubator (1), its characterized in that: the fixed cover in inner chamber of artificial containers (1) is equipped with fixed damping cover (2), the fixed cover in the outside of artificial containers (1) is equipped with down vacuum cover (5), the top of artificial containers (1) is provided with top cap (6), the fixed cover in the inboard of top cap (6) is equipped with movable damping cover (7), the fixed cover in the outside of top cap (6) is equipped with vacuum cover (8), the fixed cover in the inside of top cap (6) and last vacuum cover (8) is equipped with air supply pipe (9), the fixed cover in the inside of going up vacuum cover (8) and top cap (6) is equipped with blast pipe (10).

2. A cell culture apparatus for immunotherapy of tumors according to claim 1, wherein: the inner chamber of blast pipe (10) is fixed with support ring (11), the fixed cover in inner chamber of blast pipe (10) is equipped with shutoff ring (17), the fixed cover in inner chamber of support ring (11) is equipped with screw post (12), the bottom swing joint of screw post (12) has connection pad (14), the bottom surface fixed mounting of connection pad (14) has connecting spring (15), the bottom fixedly connected with shutoff dish (16) of connecting spring (15), the top fixed mounting of screw post (12) has knob (13).

3. A cell culture assembly according to claim 1, wherein the cell culture assembly comprises: the top surfaces of the lower vacuum sleeve (5), the incubator (1) and the fixed damping sleeve (2) are located on the same horizontal plane, and the bottom surfaces of the upper vacuum sleeve (8) and the capping cover (6) are located on the same horizontal plane.

4. A cell culture assembly according to claim 1, wherein the cell culture assembly comprises: the friction lines (3) are arranged on the outer side of the fixed damping sleeve (2) and the inner side of the movable damping sleeve (7), and the bottom end of the movable damping sleeve (7) is movably sleeved between the outer side of the fixed damping sleeve (2) and the inner side of the incubator (1).

5. A cell culture apparatus for immunotherapy of tumors according to claim 1, wherein: the bottom ends of the air supply pipe (9) and the exhaust pipe (10) extend downwards to the lower part of the top surface of the inner cavity of the top sealing cover (6), and the bottom ends of the air supply pipe (9) and the exhaust pipe (10) are positioned above the bottom surfaces of the top sealing cover (6) and the upper vacuum sleeve (8).

6. A cell culture assembly according to claim 2, wherein the cell culture assembly comprises: the knob (13) is positioned above the exhaust pipe (10), and the threaded column (12) and the connecting disc (14) are positioned on the same vertical line with the axis of the plugging disc (16) and the axis of the exhaust pipe (10).

7. A cell culture assembly according to claim 2, wherein: the bottom surface of shutoff dish (16) and the top surface laminating of shutoff ring (17), the diameter value of shutoff dish (16) is greater than the internal diameter value of shutoff ring (17).

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