CN218620808U - Constant-temperature self-sterilization CO2 incubator suitable for roller bottle adherent cell culture - Google Patents
Constant-temperature self-sterilization CO2 incubator suitable for roller bottle adherent cell culture Download PDFInfo
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- CN218620808U CN218620808U CN202221589375.2U CN202221589375U CN218620808U CN 218620808 U CN218620808 U CN 218620808U CN 202221589375 U CN202221589375 U CN 202221589375U CN 218620808 U CN218620808 U CN 218620808U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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Abstract
The utility model discloses a constant temperature self-sterilization CO suitable for roller bottle adherent cell culture 2 The incubator comprises an incubator body; this internal cell culture cavity that is equipped with of incubator, cell culture cavity top is equipped with cell culture case control system, the cell culture cavity openly is equipped with a body, is equipped with the support in the cell culture cavity, the support on be equipped with a plurality of roller bottle axis of rotation, form small-size the cavity of placing between two adjacent roller bottle axis of rotation, place the roller bottle in the small-size cavity of placing, cell culture cavity bottom is equipped with the sterilization structure. The utility model aims at providing a constant temperature CO which is suitable for small scale adherent cell roller bottle culture and can carry out self-disinfection and sterilization, and has simple structure and convenient operation 2 An incubator.
Description
Technical Field
The utility model relates to the field of adherent cell culture, in particular to a constant temperature self-sterilization CO suitable for roller bottle adherent cell culture 2 An incubator.
Background
The culture of adherent cells is mainly performed in cell culture flasks, cell factories and roller bottles. The whole process of the cell culture bottle and the cell factory is immersed in the culture medium during the cell culture process, and a part of time of the adherent cells cultured in the roller bottle is directly exposed to the air environment in the roller bottle after leaving the culture medium; in addition, when digesting adherent cells, digestive enzymes are added into the culture bottle and the cell factory and then the culture bottle and the cell factory are placed in an incubator for standing, and the roller bottle is in a continuous rotating dynamic environment; thirdly, the stability of the cell culture batches varies during the cell culture process due to the abnormal level of the culture plane or the uneven distribution of the culture medium and other liquids. The large-scale industrialized roller bottle culture of adherent cells cannot be completely simulated by using a cell culture bottle or a cell factory in a small test and a pilot test. If the existing roller bottle culture device is directly adopted to carry out small tests and pilot tests, a large cell culture space needs to be prepared, and the construction and operation costs are too high.
The prior art searches can know that: CN201920575740.6 a CO2 incubator, the power distribution box comprises a box body, the top fixed mounting of box has the humidifier, the downside is provided with the fixed plate in the box, the fixed plate top is provided with the ventilated membrane, be provided with the cavity between ventilated membrane and the fixed plate, install the heating machine between fixed plate and the bottom half, water tank and refrigerator, be provided with in the upper end of ventilated membrane in the box and put the thing board, both sides fixedly connected with supporting shoe in the box, the supporting shoe with put thing board sliding connection, it has a plurality of to put the thing piece to put on the thing board rigid coupling, it sets up flutedly on the thing piece to put, the universal wheel is installed to two bisymmetries of bottom half, the top of universal wheel is provided with the mounting bracket, the top welding of mounting bracket has the turning block, be provided with the axis of rotation on the lateral wall of turning block, the universal wheel passes through the axis of rotation and is connected with the box rotation, the left and right sides of box all is provided with the baffle, the baffle passes through bayonet lock and is connected with the box rotation, the bayonet lock is located directly over the turning block. The CO2 incubator of the utility model is convenient to operate.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to use cell culture bottle or cell factory can't simulate the large-scale industrialization roller bottle of adherent cell completely and cultivate when lab scale, pilot scale. If the existing roller bottle culture device is directly adopted to carry out small tests and pilot tests, a large cell culture space needs to be prepared, and the construction and operation costs are too high.
In order to solve the above technical problem, the utility model discloses a following technical means:
constant-temperature self-sterilization CO suitable for roller bottle adherent cell culture 2 The incubator comprises an incubator body; this internal cell culture cavity that is equipped with of incubator, cell culture cavity top is equipped with cell culture case control system, the cell culture cavity openly is equipped with a body, is equipped with the support in the cell culture cavity, the support on be equipped with a plurality of roller bottle axis of rotation, form small-size the cavity of placing between two adjacent roller bottle axis of rotation, place the roller bottle in the small-size cavity of placing, cell culture cavity bottom is equipped with the sterilization structure.
The utility model aims at providing a simple structure, simple operation be applicable to can carry out oneself disappear of small-scale adherence cell roller bottle cultivationConstant temperature CO for poison and sterilization 2 An incubator.
Preferably, the present invention further provides:
the door body for the heat preservation insulated door, the positive opening size of the cell culture cavity of heat preservation insulated door size and incubator body matches each other, shaft body connection is passed through to heat preservation insulated door one end in incubator body one side, the heat preservation insulated door is opened or is closed along the shaft body and makes the cell culture cavity form sealed cavity.
The door body is internally provided with a cell culture box glass observation door.
The support be four layers, six roller bottles can be placed to every layer of support.
The cell culture cavity be equipped with insulation construction all around, insulation construction be water jacket formula heat preservation.
The sterilization structure is a sterilization water tray, the sterilization water tray is placed below the bracket, and sterilization deionized water is filled in the sterilization water tray.
The top of the incubator body is provided with a cell incubator rotating shaft transmission power box and an integrated uninterruptible power supply, and the cell incubator rotating shaft transmission power box and the integrated uninterruptible power supply are connected with a roller bottle rotating shaft through a power line and a data line.
Cell culture case control system include temperature display, CO2 concentration display, roller bottle rotational speed display, audible-visual annunciator, incubator work and set for display and operation button, temperature display, CO2 concentration display, roller bottle rotational speed display, audible-visual annunciator, incubator work set for the display and be the LED display screen, operation button set up in the below of temperature display, CO2 concentration display, roller bottle rotational speed display, audible-visual annunciator, incubator work set for the display.
The rotating speed working threshold of the rotating shaft of the roller bottle is 0-10rpm.
The temperature of the cell culture cavity is maintained above 30 ℃ for 6-8 hours, and the incubator body is provided with a buzzer alarm and a flashing red alarm lamp.
The invention has simple structure and convenient operation, can meet the requirement of small-scale adherent cell roller bottle culture without opening a cell culture room, saves the production cost and improves the production efficiency.
Drawings
FIG. 1 is a front view of the cell culture apparatus of the present invention.
FIG. 2 is a side view of the cell culture chamber of the present invention.
FIG. 3 is a schematic view of the controller of the cell incubator of the present invention.
Description of reference numerals: 1-cell culture chamber; 2-cell incubator control system; 3-heat preservation and insulation door; 12-cell incubator glass viewing door; 13-water jacket type heat insulation layer; 14-roller bottle rotating shaft; 15-rolling the bottle; 16-sterilized water tray; 21-cell incubator rotating shaft transmission power box; 22-integrated uninterruptible power supply; 23-a temperature display; 24-CO 2 A concentration display; 25-roller bottle rotation speed display; 26-audible and visual alarm; 27-operating keys; 28-incubator working setup display.
Detailed Description
The present invention will be further described with reference to the following examples.
The specific embodiment is as follows:
referring to fig. 1, 2 and 3, the cell culture box 1 of the present invention has 4 layers of roller bottle supports, and 6 roller bottles can be placed on each layer; the water jacket type heat insulation layer can ensure that the environmental temperature is more stable in the cell culture process; meanwhile, an uninterruptible power supply is integrated in the incubator, so that the temperature in the incubator can be maintained at more than 30 ℃ for 6-8 hours in a closed state of a heat insulation door of the cell incubator in a power-off state. Sterile deionized water is poured into the sterile water tray 16, so that the saturated humidity in the incubator can be kept during the working period of the incubator; after the incubator heat-insulating door 3 is opened, the operating state of the roller bottle can be directly observed without opening the glass observation door 12. Temperature display 23, CO 2 The concentration display 24 and the rotating speed display 25 display set parameters when the cultivation wants to be set, and display running parameters in real time when the worktable is on; the incubator working setting display 28 can display a plurality of setting parameters including the service life and replacement of the air filter element, the setting of the rotating speed of the roller bottle rotating shaft, and the temperatureSetting and calibration, CO 2 Setting and calibrating concentration, and self-cleaning and sterilizing the incubator body. The rotating speed working threshold of the rotating shaft 14 of the roller bottle of the incubator is 0-10rpm, and the requirement of culture and pancreatin digestion of various adherent cells can be met. When the working parameters of the cell incubator exceed the range of the set working parameters, the incubator can send out buzzing sound and twinkle red alarm lamps, and send out an alarm to an administrator in real time through an accessed laboratory management system.
Since the above description is only a specific embodiment of the present invention, but the protection of the present invention is not limited thereto, any person skilled in the art can think of equivalent changes or substitutions of the technical features of the present invention, and all are covered in the protection scope of the present invention.
Claims (7)
1. Constant-temperature self-sterilization CO suitable for roller bottle adherent cell culture 2 The incubator comprises an incubator body; the method is characterized in that: this internal cell culture cavity that is equipped with of incubator, cell culture cavity top is equipped with cell culture case control system, the cell culture cavity openly is equipped with a body, is equipped with the support in the cell culture cavity, the support on be equipped with a plurality of roller bottle axis of rotation, form small-size cavity of placing between two adjacent roller bottle axis of rotation, place the roller bottle in the small-size cavity of placing, cell culture cavity bottom is equipped with the sterilization structure, the door body be the thermal-insulated door that keeps warm, the positive opening size of the cell culture cavity of thermal-insulated door size and incubator body matches each other, thermal-insulated door one end is passed through the axis body and is connected in incubator body one side, the thermal-insulated door that keeps warm opens or closed messenger cell culture cavity along the axis body and forms seal chamber, the support be four layers, six roller bottles can be placed to every layer of support, the sterilization structure be sterilization water tray, the sterilization water tray is placed in the support below, fills the sterilization deionized water in the sterilization water tray.
2. The isothermal self-sterilizing CO suitable for roller bottle adherent cell culture according to claim 1 2 Incubator, its characterized in that: the door body is internally provided with cell culture box glass for observationAnd a door.
3. The isothermal self-sterilizing CO suitable for roller bottle adherent cell culture according to claim 1 2 Incubator, its characterized in that: the cell culture cavity be equipped with insulation construction all around, insulation construction be water jacket formula heat preservation.
4. The isothermal self-sterilizing CO suitable for roller bottle adherent cell culture according to claim 1 2 Incubator, its characterized in that: the top of the incubator body is provided with a cell incubator rotating shaft transmission power box and an integrated uninterruptible power supply, and the cell incubator rotating shaft transmission power box and the integrated uninterruptible power supply are connected with a roller bottle rotating shaft through a power line and a data line.
5. The isothermal self-sterilizing CO suitable for roller bottle adherent cell culture according to claim 1 2 Incubator, its characterized in that: the cell culture box control system comprises a temperature display and CO 2 Concentration display, roller bottle rotating speed display, audible and visual alarm, incubator working setting display and operation keys, temperature display, CO 2 The concentration display, the roller bottle rotating speed display, the audible and visual alarm and the incubator working setting display are all LED display screens, and the operation keys are arranged on the temperature display and the CO 2 A concentration display, a roller bottle rotating speed display, an audible and visual alarm and an incubator working setting display.
6. The isothermal self-sterilizing CO suitable for roller bottle adherent cell culture according to claim 1 2 Incubator, its characterized in that: the rotating speed working threshold of the rotating shaft of the roller bottle is 0-10rpm.
7. The isothermal self-sterilizing CO suitable for roller bottle adherent cell culture according to claim 1 2 Incubator, its characterized in that: the temperature of the cell culture cavity is maintained above 30 ℃ for 6-8 hours, and the incubator body is provided withA buzzer alarm and a flashing red alarm lamp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221589375.2U CN218620808U (en) | 2022-06-24 | 2022-06-24 | Constant-temperature self-sterilization CO2 incubator suitable for roller bottle adherent cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221589375.2U CN218620808U (en) | 2022-06-24 | 2022-06-24 | Constant-temperature self-sterilization CO2 incubator suitable for roller bottle adherent cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218620808U true CN218620808U (en) | 2023-03-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221589375.2U Active CN218620808U (en) | 2022-06-24 | 2022-06-24 | Constant-temperature self-sterilization CO2 incubator suitable for roller bottle adherent cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218620808U (en) |
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2022
- 2022-06-24 CN CN202221589375.2U patent/CN218620808U/en active Active
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