CN218435776U - Observation objective table cell culture case of normal position - Google Patents
Observation objective table cell culture case of normal position Download PDFInfo
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- CN218435776U CN218435776U CN202222213387.1U CN202222213387U CN218435776U CN 218435776 U CN218435776 U CN 218435776U CN 202222213387 U CN202222213387 U CN 202222213387U CN 218435776 U CN218435776 U CN 218435776U
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Abstract
The utility model discloses an objective table cell culture case is surveyd to normal position in cell culture technical field aims at solving and can't carry out normal position observation and operation scheduling problem in cell culture among the prior art, and it includes: the cell culture box body is arranged inside the outer containing box body, a culture plate is arranged at the bottom of the cell culture box body, a steel plate is embedded on a bottom plate, below the culture plate, of the outer containing box body, the steel plate is connected with one end of a hose through a flange, and the other end of the hose is connected with a lens. The periphery of the hose is connected with a stress loading system which is used for adjusting the stretching stress to which the cells are subjected. One side of the cell culture box body is provided with a carbon dioxide inlet which is sequentially connected with an external carbon dioxide purifier and a carbon dioxide generator through pipelines. The utility model discloses not only provide the environment that more does benefit to cell growth, can realize the real-time microscopic observation of cell normal position and operation in cell culture moreover.
Description
Technical Field
The utility model relates to an objective table cell culture case is surveyd to normal position belongs to cell culture technical field.
Background
Cell biology is the subject of studying the life process of cells, early cytology is mainly described by morphology, later cytology absorbs the achievements of molecular biology, and goes deep to the level of ultrastructure, mainly studying the biological processes of cell growth, metabolism, heredity and the like. Cytological experiments are a frequently involved link in the field of cell biology, life sciences and related medical research. The living cells are basic structural units and functional units of life bodies, so that the living cells have important significance for real-time observation of single living cells. In order to observe activities, migration, proliferation and other behaviors of cells for a long time in real time, a cell culture device matched with a microscope stage structure is required.
However, the current cell culture device is difficult to realize the experimental requirement of real-time observation while culturing cells in terms of volume and structure, some foreign object stage culture devices can realize in-situ observation of a single cell culture process, but are expensive, low in integration level with other operation modules, not in totally-enclosed cooperation with an objective lens, limited in internal space or more in pipeline complex peripheral equipment, and inconvenient for simultaneously culturing and observing multiple groups of cell samples.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the not enough among the prior art, provide an objective table cell culture case is surveyd to normal position, not only provide the environment that more does benefit to cell growth, can realize the real-time microscopic observation of cell normal position and operation, convenient to use in cell culture moreover.
In order to achieve the purpose, the utility model adopts the following technical proposal: the utility model provides an objective table cell culture case is surveyd to normal position, contains box and cell culture box including the outside, the cell culture box is located the outside and is contained inside the box, the cell culture bottom of the case portion is equipped with the cultivation board, the outside contains the box and is located to inlay on the bottom plate of cultivation board below and is equipped with the steel sheet, the steel sheet passes through the flange and is connected with hose one end, the hose other end is connected with the camera lens.
The periphery of the hose is connected with a stress loading system, and the stress loading system is used for regulating the stretching stress to which the cells are subjected.
And a carbon dioxide air inlet is formed in one side of the cell culture box body and is sequentially connected with an external carbon dioxide purifier and a carbon dioxide generator through pipelines.
Optionally, the stress loading system includes a pressure sensor, a vacuum pump and a PDMS film, the pressure sensor is connected to the large air outlet on one side of the hose through a pipeline, the PDMS film is disposed on the upper portion of the flange, the vacuum pump is connected to the small air outlet on one side of the hose through a pipeline, and the vacuum pump can pump the enclosed space below the PDMS film to a negative pressure under the control of the control system, so as to adjust the tensile stress applied to the cells.
Optionally, the pressure sensor is arranged inside the sensor cabin, the sensor cabin includes a sensor cabin body and a sensor cabin cover, and a groove for placing the pressure sensor is arranged in the sensor cabin body.
Optionally, the cell culture device further comprises a carbon dioxide concentration sensor for monitoring the concentration of carbon dioxide in the cell culture box body, wherein the carbon dioxide concentration sensor is arranged on one side of the cell culture box body.
Optionally, a pressure reducing valve and an electromagnetic valve are connected between the carbon dioxide purifier and the carbon dioxide generator, and the carbon dioxide concentration sensor, the pressure reducing valve and the electromagnetic valve are all electrically connected with the carbon dioxide concentration controller.
Optionally, a first sinking groove for placing a culture dish is arranged below the culture plate.
Optionally, one side of the bottom of the cell culture box body is provided with a second settling tank, the second settling tank is used for placing a temperature sensor, and the temperature sensor is also electrically connected with the temperature controller.
Optionally, the inner wall of the cell culture box body is provided with an aluminum plate and a hot plate from inside to outside, and the aluminum plate and the hot plate are used for heat preservation.
Optionally, the heating device further comprises a heating film, wherein one part of the heating film is attached to the outer surface of the steel plate, and the other part of the heating film is covered on the outer wall of the hose or attached to the inner wall of the hose.
Optionally, the external containing box body and the cell culture box body are both provided with cover plates, and the cover plates of the cell culture box body are connected with the cell culture box body through bolt structures;
the external containing box body and the cell culture box body are respectively provided with a plurality of through holes, and the through holes are used for input and output of each line and pipeline.
Compared with the prior art, the utility model discloses the beneficial effect who reaches:
the utility model is connected with the hose through the flange at the bottom of the cell culture box body, the hose is connected with the observation lens, the cell in-situ real-time in-situ microscopic observation can be conveniently realized during the cell culture, the operation is simple, and the use is convenient;
a stress loading system is arranged below the cell culture box body, the air pressure of the closed space below the PDMS film can be pumped to negative pressure under the control of the control system through a vacuum pump, the PDMS film can be deformed, the stretching stress on the cells can be adjusted, and the stress stimulation to the cells can be realized;
the utility model discloses be provided with the heating film for the bottom surface also is in the state of heating under the culture plate of cell is equipped with, is favorable to surviving and growing of cell more, is equipped with aluminum plate and hot plate at cell culture box inner wall simultaneously, also is favorable to the stability of the temperature in the cell culture box when reinforcing heat conduction effect.
Drawings
Fig. 1 is a schematic diagram of an internal structure of a cell culture box of an in-situ observation stage according to an embodiment of the present invention;
fig. 2 is a schematic bottom view of a cell incubator with an in-situ observation stage according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of an external structure of a cell incubator with an in-situ observation stage according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a sensor chamber of a cell culture box of an in-situ observation objective table according to an embodiment of the present invention;
fig. 5 is a schematic diagram of a connection structure of a carbon dioxide gas supply and concentration control system of a cell culture box of an in-situ observation objective table according to an embodiment of the present invention;
in the figure: 1 external containing box body, 2 cell culture box body, 3 wire inlet, 4 carbon dioxide gas inlet, 5 sink groove I, 6 sink groove II, 7 carbon dioxide gas concentration sensor, 8 culture plate, 9 temperature sensor, 10 hot plate, 11 aluminum plate, 12 steel plate, 13 flange, 14 hose, 15 observation lens, 16 heating film, 17 sensor cabin cover, 18 pressure sensor, 19 sensor cabin body, 20 vacuum pump, 21 carbon dioxide generator, 22 pressure reducing valve, 23 electromagnetic valve and 24 carbon dioxide purifier.
Detailed Description
The present invention will be further described with reference to the accompanying drawings. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
Example 1:
as shown in fig. 1-2, the cell culture box of the in-situ observation objective table provided by the embodiment of the present invention comprises: the cell culture box body 2 is arranged inside the outer containing box body 1, and the cell culture box body 2 and the outer containing box body have the same bottom plate and are formed by bonding black opaque acrylic plates. The outer containing box body 1 and the cell culture box body 2 are both provided with cover plates, one side of the cover plate of the cell culture box body 2 is connected with the cell culture box body 2 through a hinge, and the other side of the cover plate is connected through a locking buckle, so that the cover plate is convenient to open and close. The external containing box body 1 and the cell culture box body 2 are both provided with a plurality of through holes which are used for input and output of each line and pipeline.
The bottom plate of the external containing box body 1 below the culture plate 8 is embedded with a steel plate 12, the steel plate 12 is connected with one end of a hose 14 through a flange 13, the other end of the hose 14 is connected with an observation lens 15, and the observation lens 15 is convenient for in-situ real-time microscopic observation of the cell culture process. The outer surface of the steel plate 12 is adhered with a heating film 16, the other part of the heating film 16 is coated on the outer wall of the hose 14, one side of the bottom of the steel plate is provided with a second sinking groove 6, the second sinking groove 6 can be used for placing a temperature sensor 9, and the temperature sensor 9 can monitor the temperature in the cell culture box body 2.
According to the actual need of cell culture and the monitoring result of combining temperature sensor 9, can heat the box through heating film 16, be equipped with aluminum plate 11 and hot plate 10 simultaneously at cell culture box 2 inner wall from inside to outside, aluminum plate 11 and hot plate 10 all can be used to keep warm, guarantee the stability of the temperature in the cell culture box 2.
Example 2:
with reference to fig. 3, on the basis of embodiment 1, the outer periphery of the hose 14 of this embodiment is connected to a stress loading system, the stress loading system includes a pressure sensor 18, a vacuum pump 20 and a PDMS film, the pressure sensor 18 is connected to the large air outlet on one side of the hose 14 through a pipe, and is limited to the volume factor of the pressure sensor 18, and cannot be placed inside the hose 14, so the pressure sensor 18 is disposed inside the sensor chamber, with reference to fig. 4, the sensor chamber includes a sensor chamber body 19 and a sensor chamber cover 17, the pressure sensor 18 is placed in a groove of the sensor chamber body 19, and after the placement, a gap is sealed with a hot melt adhesive.
The PDMS film is arranged on the upper portion of the flange 13, the vacuum pump 20 is connected with the small air outlet on one side of the hose 14 through a pipeline and matched with monitoring data of the pressure sensor 18, the vacuum pump 20 can pump negative pressure in a closed space below the PDMS film under the control of the control system, so that the PDMS film is deformed, and therefore the tension stress applied to cells is adjusted, and the growth of the cells is facilitated.
Example 3:
as shown in FIG. 5, in addition to example 1, this example was provided with a carbon dioxide gas supply and concentration control system, and a carbon dioxide gas inlet was provided on the cell culture enclosure 2 side, and the carbon dioxide gas inlet was connected to an external carbon dioxide purifier 24 and a carbon dioxide generator 21 in this order via pipes. The carbon dioxide purifier 24 functions to further filter the carbon dioxide gas supplied from the gas supply side of the carbon dioxide generator 21, thereby increasing the purity of the carbon dioxide gas entering the cell culture chamber 2.
A pressure reducing valve 22 and an electromagnetic valve 23 are connected between the carbon dioxide purifier 24 and the carbon dioxide generator 21, and the concentration c of carbon dioxide gas in the incubator body and the valve opening d of the carbon dioxide gas inlet control electromagnetic valve 23 have a functional relation:
d＝k(E-log a c)；E＝log a c max ；
wherein k is a proportionality coefficient, c max The maximum gas concentration of the system, a, is constant, i.e., the valve opening d of the carbon dioxide inlet control solenoid valve 23 decreases with increasing value of c as the carbon dioxide gas concentration c in the cell culture chamber 2 increases.
A carbon dioxide concentration sensor 7 is also arranged on one side of the cell culture box body 2, and a probe of the carbon dioxide concentration sensor 7 is in contact with the internal gas environment through a through hole on the cell culture box body 2, so that the carbon dioxide concentration of the cell culture box body can be monitored.
The carbon dioxide concentration sensor 7, the reducing valve 22 and the electromagnetic valve 23 are all electrically connected with the carbon dioxide concentration controller. The carbon dioxide controller takes the time required for the natural diffusion of the carbon dioxide gas in the cell culture box body 2 into consideration according to the program design, and finally realizes the maintenance of the concentration c of the carbon dioxide gas in the culture box body at a set value through the high-frequency concentration feedback and the gas diffusion characteristic adaptability program design.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The utility model provides an objective table cell culture case is surveyed to normal position which characterized in that: the cell culture box body is arranged inside the external containing box body, a culture plate is arranged at the bottom of the cell culture box body, a steel plate is embedded on a bottom plate of the external containing box body below the culture plate, the steel plate is connected with one end of a hose through a flange, and the other end of the hose is connected with an observation lens;
the periphery of the hose is connected with a stress loading system, and the stress loading system is used for regulating the stretching stress to which the cells are subjected;
and a carbon dioxide air inlet is formed in one side of the cell culture box body and is sequentially connected with an external carbon dioxide purifier and a carbon dioxide generator through pipelines.
2. The in situ observation stage cell culture chamber of claim 1, wherein: the stress loading system comprises a pressure sensor, a vacuum pump and a PDMS film, wherein the pressure sensor is connected with a large air outlet at one side of the hose through a pipeline, the PDMS film is arranged at the upper part of the flange, the vacuum pump is connected with a small air outlet at one side of the hose through a pipeline, and the vacuum pump can pump the closed space below the PDMS film to negative pressure under the control of the control system, so that the tension stress applied to the cells is adjusted.
3. The in situ observation stage cell culture chamber of claim 2, wherein: the pressure sensor is arranged in the sensor cabin, the sensor cabin comprises a sensor cabin body and a sensor cabin cover, and a groove for placing the pressure sensor is formed in the sensor cabin body.
4. The in situ observation stage cell culture chamber of claim 1, wherein: the carbon dioxide concentration sensor is used for monitoring the concentration of carbon dioxide in the cell culture box body and is arranged on one side of the cell culture box body.
5. The in situ observation stage cell culture box of claim 4, wherein: and a pressure reducing valve and an electromagnetic valve are connected between the carbon dioxide purifier and the carbon dioxide generator, and the carbon dioxide concentration sensor, the pressure reducing valve and the electromagnetic valve are all electrically connected with the carbon dioxide concentration controller.
6. The in situ observation stage cell culture box of claim 1, wherein: and a first sinking groove for placing a culture dish is arranged below the culture plate.
7. The in situ observation stage cell culture chamber of claim 1, wherein: and one side of the bottom of the cell culture box body is provided with a second settling tank, the second settling tank is used for placing a temperature sensor, and the temperature sensor is also electrically connected with the temperature controller.
8. The in situ observation stage cell culture box of claim 1, wherein: the inner wall of the cell culture box body is provided with an aluminum plate and a hot plate from inside to outside.
9. The in situ observation stage cell culture box of claim 1, wherein: the heating device further comprises a heating film, wherein one part of the heating film is attached to the outer surface of the steel plate, and the other part of the heating film is coated on the outer wall of the hose or attached to the inner wall of the hose.
10. The in situ observation stage cell culture chamber of claim 1, wherein: the external containing box body and the cell culture box body are both provided with cover plates, and the cover plates of the cell culture box body are connected with the cell culture box body through a bolt mechanism;
the external containing box body and the cell culture box body are respectively provided with a plurality of through holes, and the through holes are used for input and output of each line and pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222213387.1U CN218435776U (en) | 2022-08-23 | 2022-08-23 | Observation objective table cell culture case of normal position |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222213387.1U CN218435776U (en) | 2022-08-23 | 2022-08-23 | Observation objective table cell culture case of normal position |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218435776U true CN218435776U (en) | 2023-02-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222213387.1U Active CN218435776U (en) | 2022-08-23 | 2022-08-23 | Observation objective table cell culture case of normal position |
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| Country | Link |
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| CN (1) | CN218435776U (en) |
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- 2022-08-23 CN CN202222213387.1U patent/CN218435776U/en active Active
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