CN218290883U - Assembly for batch accelerated cell resuscitation - Google Patents
Assembly for batch accelerated cell resuscitation Download PDFInfo
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- CN218290883U CN218290883U CN202222380498.1U CN202222380498U CN218290883U CN 218290883 U CN218290883 U CN 218290883U CN 202222380498 U CN202222380498 U CN 202222380498U CN 218290883 U CN218290883 U CN 218290883U
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Abstract
An assembly for accelerating cell recovery in batches comprises a frame body and a pipe fitting. The frame body is provided with a plurality of through holes which are arranged in a transverse or longitudinal direction to form an array, one side which is in contact with water is provided with a plurality of through grooves, and the through holes which are arranged in the transverse direction are communicated with each other along the through grooves. The pipe fitting is used for depositing the cell, and it includes body and lid, lid and body assembly, and in the through-hole was arranged in to the body, the lid was located the through-hole top, sets up the heat transfer district on the lateral wall of body, and the heat transfer district includes 2 at least cell bodies, and each cell body is the arc, sets up around the lateral wall of body. The utility model discloses a subassembly has not only improved the volume of cell recovery synchronous operation, and efficiency is showing and is improving, more makes each freezing pipe be heated more evenly, and the different positions of freezing pipe be heated more unanimously, compares with freezing before depositing, and the cell survival rate after the recovery does not see obvious reduction, has kept the survival rate of cell after the recovery well.
Description
Technical Field
The present invention relates to a heat transfer device, and more particularly to an assembly for performing rapid bulk freezing and thawing of frozen biological products to reduce the time for cell resuscitation.
Background
After cells are extracted from tissues such as human placenta and umbilical cord, it is generally necessary to store the cells in a cryopreservation tube and then freeze-store the cells at-196 ℃ for a long period of time. When the cells are to be used for subsequent experiments or clinical infusion, the cryopreserved tubes with the cells are taken out of the liquid nitrogen and are revived in a water bath. The experimenter holds the freezing tube and shakes back and forth in a water bath at 37 ℃ to enable the cell suspension to be restored to a fluid state from a frozen state as soon as possible, the resuscitation is generally completed within 1min to ensure the cell survival rate to the maximum extent, and the process is called as resuscitation.
In the recovery operation process, each experimenter can recover 2-4 cryopreserved tubes at one time, and even if a test tube rack can be adopted, the requirement of batch recovery cannot be met. In addition, when a large number of freezing tubes (such as clinical cell infusion, animal experiment, etc., usually at least dozens of freezing tubes need to be recovered at one time, or even dozens of freezing tubes) are placed at one position, local heat exchange insufficiency of the freezing tubes occurs, particularly the freezing tube positioned in the middle and the contact part between every two freezing tubes, so that the recovery time is prolonged, the efficiency is low, and the cell survival rate is reduced because the contact time between the firstly recovered cells and the liquid freezing solution is too long and the next treatment is not carried out in time.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a subassembly for quickening cell recovery in batches improves the heat transfer, satisfies the needs of recovering in batches.
Another object of the utility model is to provide a subassembly for quickening cell recovery in batches improves the efficiency of recovering in batches.
Still another object of the present invention is to provide an assembly for accelerating cell resuscitation in batches, so as to facilitate maintaining cell viability.
The utility model provides a subassembly for quickening cell recovery in batches, include:
the frame body is provided with a plurality of through holes which are arranged in a transverse or longitudinal direction to form an array, one side of the frame body which is contacted with water is provided with a plurality of through grooves, and the through holes which are arranged in the transverse direction are communicated along the through grooves;
the pipe fitting for deposit the cell, it includes body and lid, lid and body assembly, in the through-hole was arranged in to the body, the lid was located the through-hole top, sets up the heat transfer district on the lateral wall of body, and the heat transfer district includes 2 at least cell bodies, and each cell body is the arc, sets up around the lateral wall of body.
In order to prevent the pipe fitting from deviating from the through hole due to the buoyancy of liquid, the utility model discloses a subassembly still includes the apron, and it is detained on the support body, or links to each other apron and support body through the screw.
In order to facilitate the transportation and the recovery operation, the utility model discloses a subassembly still includes the handle, and it is connected with support body or apron.
In order to improve the mass transfer efficiency and facilitate the flow of cold flow and hot flow, a pair of slope bodies is arranged on the edge of one side of the through hole, which is in contact with water, the slope surface of each slope body faces the pipe body, and the inclination angle of the slope surface is 110-135 degrees.
An embodiment of an assembly for use in accelerated batch cell resuscitation has a heat transfer region upper edge spaced a distance from an opening edge of a tube body less than a heat transfer region lower edge spaced a distance from a tube bottom edge of the tube body.
In another embodiment of the assembly for the batch-wise cell recovery acceleration, the ratio of the distance from the upper edge of the heat transfer area to the edge of the opening of the tube body to the distance from the lower edge of the heat transfer area to the edge of the bottom of the tube body is 1:3-1:6.
In another embodiment of the assembly for batch accelerated cell resuscitation, the tanks are arranged in parallel in the heat transfer zone.
In another embodiment of the assembly for the batch-wise accelerated cell resuscitation, the groove openings of the respective groove bodies located on the tube bodies are only oriented towards the adjacent tube bodies along the direction of the through grooves.
Drawings
FIG. 1 is a schematic view of an embodiment of an assembly for expedited batch cell resuscitation according to the present invention;
FIG. 2 is a schematic view of an angle of a frame of the assembly shown in FIG. 1;
FIG. 3 is an angled view of the cover of the assembly of FIG. 1;
FIG. 4 is an angled view of the assembly of FIG. 1 shown in an inverted state;
FIG. 5 is a schematic view of the assembly of FIG. 1 shown in an inverted position from another angle;
fig. 6 isbase:Sub>A schematic cross-sectional view taken along linebase:Sub>A-base:Sub>A of fig. 5.
Detailed Description
The technical solution of the present invention is described in detail below with reference to the accompanying drawings. The embodiments of the present invention are only used for illustrating the technical solutions of the present invention and not for limiting, although the present invention is described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced with other equivalent solutions without departing from the spirit and scope of the present invention, which should be covered by the scope of the claims of the present invention.
Fig. 1 is a schematic view of an embodiment of an assembly for accelerating cell resuscitation in batches according to the present invention, fig. 4 is a schematic view of an inverted state of the assembly shown in fig. 1 at one angle, and fig. 5 is a schematic view of an inverted state of the assembly shown in fig. 1 at another angle. As shown in fig. 1, 4 and 5, the assembly for batch-accelerated cell resuscitation of the present invention includes a frame body 100, a pipe member 200, a cover plate 300 and a handle 400. Fig. 3 is a schematic view of an angle of a cover of the assembly shown in fig. 1, referring to fig. 1 and 4, as shown in fig. 3, a cover plate 400 is fastened on a frame body 100, in this embodiment, the cover plate 400 is connected to the frame body 100 by a screw 500, so that the pipe 200 is effectively prevented from being separated from the frame body 100 due to buoyancy of liquid. A handle 400 is attached to the frame 100 or to the cover 400 to move or transport the assembly during the resuscitation operation and to synchronize the operation of the frozen tubes in the batch.
Fig. 2 is a schematic view of an angle of a frame body of the assembly shown in fig. 1. Referring to fig. 1, 4 and 5, as shown in fig. 2, a plurality of through holes 110 are formed in a frame body 100, the through holes 110 are arranged in a transverse or longitudinal direction to form an array, a plurality of through grooves 120 are formed on a side contacting water, and the through grooves 120 are communicated with the through holes 110 arranged in the transverse direction.
Fig. 6 isbase:Sub>A cross-sectional view taken alongbase:Sub>A-base:Sub>A in fig. 5, and as shown in fig. 6,base:Sub>A tube 200 for storing cells includesbase:Sub>A tube 210 andbase:Sub>A cap 220, and the cap 220 is assembled with the tube 210. Each tube 210 is correspondingly disposed in one of the through holes 110, and the cover 220 is disposed above the through hole 110, for example: the edge of the cover 220 is mounted on the through hole, or the tube 210 is in interference fit with the wall of the through hole 110, so that the cover 220 is suspended above the through hole 110. A heat transfer region 211 is formed on a sidewall of the pipe body 210, and the heat transfer region 211 includes at least 2 groove bodies (not shown), each of which is arc-shaped and is disposed around the sidewall of the pipe body 210. Each slot body is arranged in parallel in the heat transfer area, and along the direction of the through slot 120, the slot opening of each slot body on the tube body 210 only faces to the adjacent tube body. The distance from the upper edge 212 of the heat transfer area to the edge of the opening of the tube body is smaller than the distance from the lower edge 213 of the heat transfer area to the edge of the bottom of the tube body, and the ratio is: 1:3-1:6 to facilitate faster flow of cold and hot streams.
In order to improve the mass transfer efficiency, facilitate the flow of cold flow and hot flow and make the freezing liquid in each position of the pipe body tend to be thawed synchronously, a pair of slope bodies 130 are arranged on the edge of one side of each through hole 110, which is in contact with water. The slope of the ramp 130 faces the body 110 to facilitate fluid flow and energy exchange at the upper portion of the body, near the orifice. When the groove opening of each groove body on the pipe body 110 only faces to the adjacent pipe body along the trend of the through groove 120, the flow of the fluid is accelerated by each groove body and the slope surface, and particularly the inclination angle of the slope surface is 110-135 degrees.
The subassembly that this embodiment provided, it has not only improved the volume of cell resuscitation synchronous operation, and efficiency is showing and is improving, more makes each freezing pipe be heated more evenly, and the different positions of a freezing pipe are also more unanimous to be heated, compares before freezing, and the cell survival rate after the recovery does not see obvious reduction, has well kept the survival rate of cell after the recovery.
Claims (9)
1. An assembly for expedited batch cell resuscitation, comprising:
the frame body is provided with a plurality of through holes which are arranged in a transverse or longitudinal direction to form an array, one side of the frame body which is contacted with water is provided with a plurality of through grooves, and the through holes which are arranged in the transverse direction are communicated along the through grooves;
the pipe fitting for deposit the cell, it includes body and lid, lid and body assembly, in the through-hole was arranged in to the body, the lid was located the through-hole top, sets up the heat transfer district on the lateral wall of body, and the heat transfer district includes 2 at least cell bodies, and each cell body is the arc, sets up around the lateral wall of body.
2. The assembly for batch accelerated cell resuscitation of claim 1, further comprising a cover plate fastened to the frame body or connected to the frame body by screws.
3. The assembly for expedited batch cell resuscitation of claim 1, further comprising a handle coupled to the frame or the cover plate.
4. The assembly for rapid batch cell resuscitation of claim 1, wherein a pair of slopes is disposed at the edge of the through hole contacting with water, and the slope of the slope faces the tube.
5. The assembly of claim 4 wherein the slope is inclined at an angle of 110 ° to 135 °.
6. The assembly of claim 1 wherein the distance from the upper edge of the heat transfer region to the edge of the opening of the tubular body is less than the distance from the lower edge of the heat transfer region to the edge of the bottom of the tubular body.
7. The assembly for expedited batch cell resuscitation of claim 1, wherein a ratio of a distance from an upper edge of the heat transfer region to an edge of the opening of the tube to a distance from a lower edge of the heat transfer region to a bottom edge of the tube is 1:3 to 1:6.
8. The assembly for enhanced batch cell resuscitation as claimed in claim 1, wherein the tanks are disposed in parallel in the heat transfer region.
9. The assembly for expedited batch cell resuscitation as claimed in claim 1, wherein the slot openings of the respective slots on the tubes are oriented toward only adjacent tubes along the direction of the through slot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222380498.1U CN218290883U (en) | 2022-09-07 | 2022-09-07 | Assembly for batch accelerated cell resuscitation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222380498.1U CN218290883U (en) | 2022-09-07 | 2022-09-07 | Assembly for batch accelerated cell resuscitation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218290883U true CN218290883U (en) | 2023-01-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222380498.1U Active CN218290883U (en) | 2022-09-07 | 2022-09-07 | Assembly for batch accelerated cell resuscitation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218290883U (en) |
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2022
- 2022-09-07 CN CN202222380498.1U patent/CN218290883U/en active Active
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