CN214932363U

CN214932363U - Reproductive sample cryopreserving device

Info

Publication number: CN214932363U
Application number: CN202120313409.4U
Authority: CN
Inventors: 胡佳霖; 李璜; 马丽琴
Original assignee: Guangzhou Pinjie Biotechnology Co ltd
Current assignee: Guangzhou Pinjie Biotechnology Co ltd
Priority date: 2021-02-03
Filing date: 2021-02-03
Publication date: 2021-11-30
Anticipated expiration: 2031-02-03

Abstract

The utility model discloses a reproductive sample cryopreservation device, belonging to the field of sample storage equipment and comprising a liquid nitrogen tank, a support frame supported in the liquid nitrogen tank and a plurality of cryopreservation frames supported in the support frame; the freezing storage rack is provided with a top plate, a bottom plate and a plurality of layers of storage cavities which are distributed up and down between the top plate and the bottom plate; the device is characterized in that the support frame comprises a support plate, the support plate vertically divides the interior of the liquid nitrogen tank into an extraction area and a storage area, and the support plate is vertically provided with a plurality of through holes corresponding to the freezing rack; a suspension shaft is fixed on a top plate of the freezing frame, and the freezing frame passes through the through hole, enters the storage area and is suspended and supported on the support plate through the suspension shaft; vacant cavities are reserved between the storage cavity at the top layer and the top plate and between the storage cavity at the bottom layer and the bottom plate in the freezing storage frame. Can ensure the storage environment of reproduction sample to will freeze and deposit the frame and promote to extract the district and get and put and freeze and deposit the box, still guaranteed dark low temperature environment.

Description

Reproductive sample cryopreserving device

Technical Field

The utility model belongs to sample storage device field, concretely relates to reproduction sample cryopreserving device.

Background

In the biomedical field, germ cells such as sperm, ovum, embryo, etc. need to be cryopreserved. Present hospital or research institution etc. generally place the liquid nitrogen jar through manual freezing of putting the sample in the box, and not only inefficiency, the storage process risk is great, need place a large amount of liquid nitrogen jars moreover usually, has occupied great space and a large amount of jar bodies, nevertheless does not reach higher memory space.

The existing freezing storage equipment is generally used for common cell and tissue samples, and the storage of the existing freezing storage equipment is realized in a liquid nitrogen gas phase environment to realize automatic storage and taking. However, the reproductive sample needs to be preserved in a liquid-phase liquid nitrogen environment, and the sample is soaked below the liquid level of the liquid nitrogen. Therefore, a device capable of storing the reproductive samples needs to be developed, and the problem of storing the reproductive samples in a liquid-phase liquid nitrogen environment in the device needs to be solved in the development process.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a reproduction sample cryopreserving device to solve the problem of depositing of reproduction sample in liquid phase liquid nitrogen environment.

For realizing the purpose of the utility model, the technical proposal adopted is that: a reproductive sample cryopreservation device comprises a liquid nitrogen tank, a support frame supported in the liquid nitrogen tank, and a plurality of cryopreservation racks supported in the support frame; the freezing storage rack is provided with a top plate, a bottom plate and a plurality of layers of storage cavities which are distributed up and down between the top plate and the bottom plate; the support frame comprises a support plate, the interior of the liquid nitrogen tank is vertically divided into an extraction area and a storage area by the support plate, and the support plate is vertically provided with a plurality of through holes corresponding to the freezing frame; a suspension shaft is fixed on a top plate of the freezing frame, and the freezing frame passes through the through hole, enters the storage area and is suspended and supported on the support plate through the suspension shaft; vacant cavities are reserved between the storage cavity at the top layer and the top plate and between the storage cavity at the bottom layer and the bottom plate in the freezing storage frame.

As a further alternative, a suspension seat is fixed on the top plate, and a plurality of suspension shafts are fixed around the suspension seat.

As a further alternative, the suspension shafts are four and distributed in a cross shape.

As a further alternative, a support block is fixed on the support plate around the through hole, and the support block is provided with a support groove matched with the suspension shaft.

As a further alternative, the height of the extraction zone is not less than the overall height of all the storage cavities.

As a further alternative, the bottom of each storage cavity in the cryopreservation rack is fixed with two cryopreservation box support bars, the two cryopreservation box support bars are respectively fixed on two sides in the cryopreservation rack, and an extraction operation gap is formed between the two cryopreservation box support bars.

As a further alternative, the two sides of each storage cavity in the freezing storage rack are provided with elastic sheets.

As a further alternative, the top of the liquid nitrogen tank is provided with an access port.

The utility model has the advantages that: the freezing rack is suspended on the supporting frame through the suspension shaft to be placed, the freezing rack is prevented from being directly placed on a flat plate at the bottom of the supporting frame, the flat plate is deformed to cause offset, so that the freezing rack is prevented from being extracted, the freezing rack is stably placed in the storage area, liquid-phase liquid nitrogen in the liquid nitrogen tank is added to the supporting plate, the freezing box is not stored in the vacant cavity, the vacant cavity at the top is used for reserving and consuming the liquid-phase liquid nitrogen, therefore, the freezing storage boxes in the storage cavities of all layers below can be always soaked in the liquid nitrogen before the liquid nitrogen needs to be supplemented, the empty cavity at the bottom prevents the freezing frame from separating from the support frame and being difficult to fall back to the original position when the freezing frame is lifted to the extraction area, meanwhile, the cryopreservation box in the storage cavity at the lowest part can be extracted, the storage is stable and reliable, the storage environment of reproductive samples can be guaranteed, and the freezing frame is lifted to the extraction area to pick and place the freezing box, so that the deep low-temperature environment is still ensured.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings required for the description of the embodiments will be briefly introduced below, it should be understood that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

Fig. 1 is a schematic structural view of a reproductive sample cryopreservation device provided by an embodiment of the present invention;

FIG. 2 is an enlarged view of a portion of the support plate of the reproductive sample cryopreservation apparatus of FIG. 1;

FIG. 3 is a schematic structural view of a cryopreservation rack of the reproductive sample cryopreservation device shown in FIG. 1;

FIG. 4 is a schematic diagram of the structure of a suspension mount in the cryopreservation rack shown in FIG. 3;

reference numerals: 1-top plate, 2-bottom plate, 3-back plate, 4-side plate, 5-storage cavity, 6-vacant cavity, 7-support bar of freezing box, 8-stop edge of freezing box, 9-suspension seat, 10-suspension shaft, 11-spring plate, 12-rib, 13-liquid nitrogen tank, 14-support frame, 15-freezing frame, 16-support plate, 17-extraction area, 18-storage area, 19-through hole, 20-flat plate, 21-support block and 22-access opening.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention. It is to be understood that the drawings are designed solely for the purposes of illustration and description and not as a definition of the limits of the invention. The connection relationships shown in the drawings are for clarity of description only and do not limit the manner of connection.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

The present invention will be further described with reference to the accompanying drawings and specific embodiments.

Fig. 1 to 4 show a reproductive sample cryopreservation device provided by the present invention, which comprises a liquid nitrogen tank 13, a support frame 14 supported in the liquid nitrogen tank 13, and a plurality of cryopreservation racks 15 supported in the support frame 14; the freezing frame 15 is provided with a top plate 1, a bottom plate 2 and a plurality of layers of storage cavities 5 which are distributed up and down between the top plate 1 and the bottom plate 2; the support frame 14 comprises a support plate 16, the support plate 16 vertically divides the interior of the liquid nitrogen tank 13 into an extraction area 17 and a storage area 18, and the support plate 16 is vertically provided with a plurality of through holes 19 corresponding to the freezing frame 15; a suspension shaft 10 is fixed on the top plate 1 of the freezing frame 15, and the freezing frame 15 passes through the through hole 19 to enter the storage area 18 and is suspended and supported on the support plate 16 through the suspension shaft 10; vacant cavities 6 are reserved between the storage cavity 5 at the top layer and the top plate 1 and between the storage cavity 5 at the bottom layer and the bottom plate 2 in the freezing storage frame 15.

The support frame 14 is rotatably supported in the liquid nitrogen tank 13, the freezing frame 15 is positioned between the support plate 16 and the flat plate 20 at the bottom, and a support rod is fixed between the support plate 16 and the flat plate 20. A gap is left between the bottom surface of the freezing frame 15 and the flat plate 20. The freezing frame 15 is provided with a back plate 3 and two side plates 4 connected between a top plate 1 and a bottom plate 2, the two side plates 4 are arranged on two sides of the back plate 3, the upper empty cavity 6 and the lower empty cavity 6 are empty, the freezing box is not placed, and the storage cavity 5 between the two empty cavities 6 is used for placing the freezing box. The height of the vacant cavity 6 can be set according to the requirement, and specifically can be not lower than the height of the storage cavity 5, and also can be set at other heights.

The bottom of every storage cavity 5 all is fixed with two and freezes deposits box support bars 7 in freezing frame 15, and two freezing box support bars 7 of depositing are fixed in the both sides in freezing frame 15 respectively, form between two freezing box support bars 7 and draw the operation clearance, for freezing box entering and shift out for freezing frame 15 provides operating space, also lightened freezing frame 15 weight simultaneously. The end of the support strip 7 of the freezing storage box, which is far away from the backboard 3, can be provided with a blocking edge 8 of the freezing storage box, so that the blocking effect is achieved, and the freezing storage box is prevented from falling off from the freezing storage frame 15.

The both sides of depositing in the chamber 5 in every in the frame 15 are deposited to freezing all are provided with shell fragment 11, apply certain two-way clamp force for freezing the case of depositing, prevent to freeze the case and appear floating in liquid phase liquid nitrogen, fasten the state of depositing of freezing the case, protect the sample.

A hanging seat 9 is fixed on the top plate 1, the hanging seat 9 can be detachably connected with the top plate 1, and a plurality of hanging shafts 10 are fixed around the hanging seat 9. The lower surface of the edge of the hanging seat 9 is provided with a convex rib 12, so that the freezing rack 15 can be stably hooked and lifted by the convex rib 12 conveniently, and the sliding is avoided. The suspension shaft 10 is detachably connected with the suspension seat 9, and can be fixedly connected through threads or by a set screw, so that the maintenance and the replacement are convenient. The four suspension shafts 10 are distributed in a cross shape, and suspension limiting in XY directions can be realized.

The supporting block 21 is fixed on the supporting plate and located around the through hole 19, the supporting block 21 is provided with a supporting groove matched with the hanging shaft 10, and the supporting groove can be an arc-shaped groove corresponding to the hanging shaft 10. The supporting block 21 is screwed with the supporting plate. The height of the extraction zone 17 is not less than the overall height of all the storage chambers 5. The access opening 22 is opened at the top of the liquid nitrogen tank 13, and the freezing storage frame 15 is lifted through the access opening 22 to carry out the picking and placing operation of the freezing storage box.

The present invention is not limited to the above-mentioned optional embodiments, and any other products in various forms can be obtained by anyone under the teaching of the present invention, and any changes in the shape or structure thereof, all the technical solutions falling within the scope of the present invention, are within the protection scope of the present invention.

Claims

1. The reproductive sample cryopreservation device comprises a liquid nitrogen tank, a support frame supported in the liquid nitrogen tank and a plurality of cryopreservation frames supported in the support frame; the freezing storage rack is provided with a top plate, a bottom plate and a plurality of layers of storage cavities which are distributed up and down between the top plate and the bottom plate; the device is characterized in that the support frame comprises a support plate, the support plate vertically divides the interior of the liquid nitrogen tank into an extraction area and a storage area, and the support plate is vertically provided with a plurality of through holes corresponding to the freezing rack; a suspension shaft is fixed on a top plate of the freezing frame, and the freezing frame passes through the through hole, enters the storage area and is suspended and supported on the support plate through the suspension shaft; vacant cavities are reserved between the storage cavity at the top layer and the top plate and between the storage cavity at the bottom layer and the bottom plate in the freezing storage frame.

2. The reproductive sample cryopreservation device as claimed in claim 1, wherein a hanging seat is fixed on the top plate, and a plurality of hanging shafts are fixed around the hanging seat.

3. The reproductive sample cryopreservation device as claimed in claim 2, wherein the number of the hanging shafts is four, and the hanging shafts are distributed in a cross shape.

4. The reproductive sample cryopreservation device as claimed in claim 1 or 2, wherein a support block is fixed on the support plate and positioned around the through hole, and the support block is provided with a support groove matched with the suspension shaft.

5. The reproductive sample cryopreservation device of claim 1 wherein the height of the extraction region is not less than the overall height of all storage cavities.

6. The reproductive sample cryopreservation device as claimed in claim 1, wherein two cryopreservation box support bars are fixed at the bottom of each storage cavity in the cryopreservation frame, the two cryopreservation box support bars are respectively fixed at two sides in the cryopreservation frame, and an extraction operation gap is formed between the two cryopreservation box support bars.

7. The reproductive sample cryopreservation device of claim 1 wherein spring plates are arranged on two sides of each storage cavity in the cryopreservation frame.

8. The cryopreservation device for the reproductive samples as claimed in claim 1, wherein an access port is formed at the top of the liquid nitrogen tank.