CN216274154U

CN216274154U - Bacillus licheniformis separation culture dish for high-yield tetramethylpyrazine

Info

Publication number: CN216274154U
Application number: CN202122494193.9U
Authority: CN
Inventors: 赵书建
Original assignee: Jinan Deruifeng Environmental Protection Chemical Co ltd
Current assignee: Jinan Deruifeng Environmental Protection Chemical Co ltd
Priority date: 2021-10-18
Filing date: 2021-10-18
Publication date: 2022-04-12
Anticipated expiration: 2031-10-18

Abstract

The utility model relates to the technical field of culture dish equipment, and discloses a separation culture dish for high-yield tetramethylpyrazine bacillus licheniformis, which solves the problem of water reduction after temperature rise in the culture dish and comprises a culture dish, wherein a culture dish base is arranged at the bottom end of the culture dish, a dish cover is arranged at the top end of the culture dish, a handle is arranged at the top end of the dish cover, a fixture block is arranged at the bottom end of the dish cover at an equal angle, a temperature sensing mechanism is arranged in the middle of the bottom end of the dish cover, a partition plate is arranged in the culture dish at an equal angle, a clamping groove is formed in the top end of the partition plate, and a water adding mechanism is arranged in the middle of the interior of the culture dish; in the utility model, in operation, the gas in the air storage cylinder expands after the temperature of the heat transfer plate is raised, then the push block is pushed to move downwards, the movable water adding column is pushed to move downwards, and then the water in the central ring enters the culture dish from the water adding hole, so that the water is automatically added into the culture dish, and the living environment of the bacillus licheniformis is adjusted.

Description

Bacillus licheniformis separation culture dish for high-yield tetramethylpyrazine

Technical Field

The utility model belongs to the technical field of culture dish equipment, and particularly relates to a separation culture dish for bacillus licheniformis for high yield of tetramethylpyrazine.

Background

Tetramethylpyrazine, also known as ligustrazine, is a bioactive component extracted from the widely used traditional Chinese medicine ligusticum wallichii. It has effects in inhibiting platelet aggregation, enhancing vasodilatation, increasing cerebral blood flow, and protecting nerve. Ligustrazine injection has been widely used in domestic for treating ischemic stroke, coronary heart disease, diabetic nephropathy, knee osteoarthritis. Ligustrazine has also been evaluated as a clinical treatment for pressure sores, as a rescue agent for non-hodgkin lymphoma patients, as a treatment for bronchial asthma and vertebrobasilar insufficiency.

During the culture of the bacillus licheniformis of tetramethylpyrazine, the temperature is higher, so that the moisture in a culture dish is reduced, the living environment of the bacillus licheniformis is changed, and the growth of the bacillus licheniformis is limited.

SUMMERY OF THE UTILITY MODEL

Aiming at the situation, in order to overcome the defects of the prior art, the utility model provides the separation culture dish for the bacillus licheniformis with high tetramethylpyrazine yield, which effectively solves the problem of water reduction after the temperature in the culture dish rises.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a high yield tetramethylpyrazine's bacillus licheniformis's isolated culture dish, includes the culture dish, the culture dish base is installed to the bottom of culture dish, and the ware lid is installed on the top of culture dish, and the handle is installed on the top of ware lid, and the fixture block is installed to angles such as the bottom of ware lid, and the mid-mounting of ware lid bottom has temperature-sensing mechanism, and the division board is installed to angles such as the inside of culture dish, and the draw-in groove has been seted up on the top of division board, and the inside mid-mounting of culture dish has the mechanism of adding water.

Preferably, the temperature sensing mechanism comprises an air storage cylinder arranged at the bottom end of the dish cover, a heat transfer plate is arranged on the outer side of the top end of the air storage cylinder, a push block is arranged inside the air storage cylinder, and a spring is arranged between the bottom end of the air storage cylinder and the top end of the push block.

Preferably, the heat transfer plate is made of aluminum.

Preferably, the mechanism of adding water is including installing at the inside centre ring of culture dish, the water hole has been seted up to angles such as the outside of centre ring, the internally mounted of centre ring has the activity to add the water column, the activity adds the mid-mounting of water column and keeps off the ring, keep off angles such as the bottom of ring and seted up the through-hole, the activity adds the bottom of water column and has seted up down the water hole, the activity adds the bottom of water column and sets up the last hole of adding water that is located down the hole top of adding water, the activity adds the bottom of water column and installs the floating block that is located down the hole below of adding water, the mid-mounting of culture dish bottom has the storage water tank.

Preferably, the movable water adding column is hollow inside, and the top end of the movable water adding column is arranged above the water adding hole.

Preferably, the floating block is arranged inside the water storage tank and is clamped with the bottom end of the culture dish.

Preferably, the fixture blocks are matched with the clamping grooves, and the number of the fixture blocks is three, and the number of the partition plates is three.

Compared with the prior art, the utility model has the beneficial effects that:

1) in the work, the internal temperature of the culture dish is transmitted to the inside of the air storage cylinder through the arranged heat transfer plate, so that the internal gas of the air storage cylinder expands, the push block is pushed to move downwards, the movable water adding column is further pushed to move downwards, the water adding hole is not blocked any more, then the water in the center ring enters the culture dish from the water adding hole, the culture dish is automatically added with water, and the living environment of the bacillus licheniformis is adjusted;

2) the utility model discloses a set up in the culture dish, in work, through angles such as the division board that sets up inside the culture dish, fixture block and draw-in groove joint simultaneously for the inside three region that forms of culture dish can carry out the isolated culture to bacillus licheniformis simultaneously then, improves cultivation efficiency.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model.

In the drawings:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the capsule structure of the present invention;

FIG. 3 is a schematic view of the structure of the culture dish of the present invention;

FIG. 4 is a schematic view of the internal structure of the culture dish of the present invention;

FIG. 5 is a schematic view of the internal structure of the active water column of the present invention.

In the figure: 1. a culture dish; 2. a culture dish base; 3. a dish cover; 4. a handle; 5. a clamping block; 6. a partition plate; 7. a card slot; 8. a temperature sensing mechanism; 801. an air cylinder; 802. a heat transfer plate; 803. a push block; 804. a spring; 9. a water adding mechanism; 901. a center ring; 902. a water filling hole; 903. a movable water adding column; 904. a baffle ring; 905. a through hole; 906. water adding holes are arranged; 907. a lower water adding hole; 908. floating blocks; 909. A water storage tank.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the first embodiment, as shown in fig. 1, fig. 2, fig. 3, fig. 4, and fig. 5, the present invention includes a culture dish 1, a culture dish base 2 is installed at the bottom end of the culture dish 1, a dish cover 3 is installed at the top end of the culture dish 1, a handle 4 is installed at the top end of the dish cover 3, a fixture block 5 is installed at the bottom end of the dish cover 3 at an equal angle, a temperature sensing mechanism 8 is installed at the middle portion of the bottom end of the dish cover 3, a partition plate 6 is installed inside the culture dish 1 at an equal angle, a clamping groove 7 is formed at the top end of the partition plate 6, and a water adding mechanism 9 is installed at the middle portion inside the culture dish 1.

In the second embodiment, the temperature sensing mechanism 8 includes an air cylinder 801 mounted to the bottom end of the dish cover 3, a heat transfer plate 802 mounted to the outside of the top end of the air cylinder 801, a push block 803 mounted to the inside of the air cylinder 801, and a spring 804 mounted between the bottom end of the air cylinder 801 and the top end of the push block 803.

In the third embodiment, the heat transfer plate 802 is made of aluminum material to improve the heat conduction efficiency.

Fourth embodiment, on the basis of the first embodiment, the watering mechanism 9 includes a center ring 901 installed inside the culture dish 1, a watering hole 902 is provided at an equal angle outside the center ring 901, a movable watering column 903 is installed inside the center ring 901, a baffle ring 904 is installed in the middle of the movable watering column 903, a through hole 905 is provided at the bottom end of the baffle ring 904 at an equal angle, a lower watering hole 907 is provided at the bottom end of the movable watering column 903, an upper watering hole 906 above the lower watering hole 907 is provided at the bottom end of the movable watering column 903, a floating block 908 below the lower watering hole 907 is installed at the bottom end of the movable watering column 903, and a water storage tank 909 is installed in the middle of the bottom end of the culture dish 1.

In the fifth embodiment, on the basis of the fourth embodiment, the movable water adding column 903 is hollow, and the top end of the movable water adding column 903 is above the water adding hole 902, so that water can be supplemented to the inside of the central ring 901 under the principle of the communicating vessel.

In the sixth embodiment, in addition to the fourth embodiment, the floating block 908 is disposed inside the water storage tank 909, the floating block 908 is engaged with the bottom end of the culture dish 1, and the floating block 908 can be adjusted back to the height and can be restrained.

Seventh embodiment, on the basis of embodiment one, fixture block 5 and draw-in groove 7 looks adaptation, fixture block 5 and division board 6 all are provided with threely, through angle settings such as the division board that sets up inside the culture dish, fixture block and draw-in groove joint simultaneously for the inside three regions that form of culture dish can carry out the isolated culture to bacillus licheniformis simultaneously then.

The working principle is as follows: during operation, at first add nutrient solution, moisture and bacterium sample in culture dish 1, cover dish lid 3 on 1 top of culture dish for fixture block 5 and draw-in groove 7 joint, dish lid 3 and culture dish 1 are fixed this moment, and ejector pad 803 bottom and activity add the contact of water column 903 top, constantly make up water in the storage water tank 909.

Constantly breed when the inside bacterium of culture dish 1 and produce a large amount of heats, then make the inside moisture evaporation of culture dish 1, make the bacterium breed the environment variation, this moment because the inside temperature of culture dish 1 risees the back, the heat spreads into in the gas receiver 801 through heat transfer plate 802, make the air in the gas receiver 801 thermal expansion, thereby make the inside atmospheric pressure increase of gas receiver 801 extrude ejector pad 803 downwards, and ejector pad 803 bottom and activity add the contact of water column 903 top, make ejector pad 803 move down and drive activity and add water column 903 downstream, then make fender ring 904 remove to add the water hole 902 below, the inside water of centre ring 901 enters into culture dish 1 through adding water hole 902 and carries out the moisture replenishment this moment.

In the downward movement process of the movable water adding column 903, the lower water adding hole 907 is moved from being closed to a position located inside the water storage tank 909, at this time, water inside the water storage tank 909 enters the movable water adding column 903 from the lower water adding hole 907, after the water inside the central ring 901 enters the culture dish 1, the temperature is reduced at this time, the air pressure inside the air cylinder 801 is reduced, under the action of the spring 804, the push block 803 moves upwards, at this time, after the movable water adding column 903 is not stressed due to the upper part, the floating block 908 floats upwards under the action of buoyancy, the lower water adding hole 907 is closed again, the upper water adding hole 906 returns to the central ring 901, under the influence of the principle of the communicating device, the water at the higher position of the movable water adding column 903 enters the central ring 901, the water inside the central ring 901 and the movable water adding column 903 are on the same horizontal plane, so that the water inside the central ring 901 is supplemented, and when the central ring 901 moves upwards, the stop ring 904 moves upward to isolate the interior of the center ring 901 from the interior of the culture dish 1, and waits for the next replenishment.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a high yield tetramethylpyrazine's bacillus licheniformis's isolated culture dish, includes culture dish (1), its characterized in that: culture dish base (2) are installed to the bottom of culture dish (1), and dish lid (3) are installed on the top of culture dish (1), and handle (4) are installed on the top of dish lid (3), and fixture block (5) are installed to angles such as the bottom of dish lid (3), and the mid-mounting of dish lid (3) bottom has temperature-sensing mechanism (8), and division board (6) are installed to angles such as the inside of culture dish (1), and draw-in groove (7) have been seted up on the top of division board (6), and culture dish (1) inside mid-mounting has water mechanism (9) of adding.

2. The separation culture dish of bacillus licheniformis for high yield of tetramethylpyrazine according to claim 1, characterized in that: the temperature sensing mechanism (8) comprises an air storage cylinder (801) arranged at the bottom end of the dish cover (3), a heat transfer plate (802) is arranged on the outer side of the top end of the air storage cylinder (801), a push block (803) is arranged inside the air storage cylinder (801), and a spring (804) is arranged between the bottom end of the air storage cylinder (801) and the top end of the push block (803).

3. The separation culture dish of bacillus licheniformis for high yield of tetramethylpyrazine according to claim 2, characterized in that: the heat transfer plate (802) is made of aluminum.

4. The separation culture dish of bacillus licheniformis for high yield of tetramethylpyrazine according to claim 1, characterized in that: water mechanism (9) including installing at inside center ring (901) of culture dish (1), water hole (902) have been seted up to angles such as the outside of center ring (901), the internally mounted of center ring (901) has activity to add water column (903), the mid-mounting of activity with water column (903) has fender ring (904), keep off angles such as the bottom of ring (904) and seted up through-hole (905), water hole (907) has been seted up down to the bottom of activity with water column (903), water hole (906) is added to the bottom of activity with water column (903) and seted up the last water hole (906) that is located down water hole (907) top, the bottom of activity with water column (903) is installed and is located the floating block (908) of adding water hole (907) below down, the mid-mounting of culture dish (1) bottom has storage water tank (909).

5. The separation culture dish of bacillus licheniformis with high tetramethylpyrazine yield according to the claim 4, characterized in that: the inside of activity water adding column (903) sets up to hollow, and the top of activity water adding column (903) is in the top of filling hole (902).

6. The separation culture dish of bacillus licheniformis with high tetramethylpyrazine yield according to the claim 4, characterized in that: the floating block (908) is arranged in the water storage tank (909), and the floating block (908) is clamped with the bottom end of the culture dish (1).

7. The separation culture dish of bacillus licheniformis for high yield of tetramethylpyrazine according to claim 1, characterized in that: the fixture blocks (5) are matched with the clamping grooves (7), and the number of the fixture blocks (5) is three, and the number of the partition plates (6) is three.