CN218249187U

CN218249187U - Ventilating filter of fermentation tank

Info

Publication number: CN218249187U
Application number: CN202222103567.4U
Authority: CN
Inventors: 高杨; 杨屹; 徐光生
Original assignee: Hefei Afana Biotechnology Co ltd
Current assignee: Hefei Afana Biotechnology Co ltd
Priority date: 2022-08-10
Filing date: 2022-08-10
Publication date: 2023-01-10
Anticipated expiration: 2032-08-10

Abstract

The utility model discloses a fermentation cylinder filter of ventilating, including the first end cover and the second end cover of mutual coincidence installation, the opposite side central authorities of first end cover and second end cover are connected with communicating pipe, and first end cover is installed and is cut off cell body and communicating pipe filtration membrane, installs the rubber ring in the second end cover, and when first end cover and second end cover coincide, rubber ring pressfitting filtration membrane avris is accomplished sealedly. First end cover and second end cover adopt polyethylene or metal material, and repeatable practicality, first end cover and second end cover can be opened and change filtration membrane, carry out high-pressure steam sterilization simultaneously, and the disinfection is more thorough, and the installation is convenient with the dismantlement, and the leakproofness is good.

Description

Ventilating filter of fermentation tank

Technical Field

The utility model belongs to the technical field of laboratory glassware, especially relate to a fermentation cylinder filter of ventilating.

Background

The current vaccine category, apart from inactivated antigens, is mostly mRNA vaccines, and the preparation process is roughly as follows: the gene recombinant strain is fermented, subjected to alkaline lysis, filtered and clarified to obtain a stock solution, the stock solution is purified by a chromatographic column to obtain a plasmid DNA template, the template is subjected to enzyme digestion and in vitro transcription to obtain mRNA, and finally the product is prepared by a Lipid Nanoparticle (LNP) technology.

Wherein, in the fermentation stage, the microorganism is a mass multiplication stage, aerobic microorganisms such as escherichia coli and the like are common, continuous ventilation is needed when the fermentation tube is cultured, in order to prevent the pollution effect of the miscellaneous source microorganisms in the ventilation gas, a ventilation filter is needed to be arranged in the middle section of the silicone tube at the air inlet end of the tank body, the existing ventilation filter is an integrated finished product and is made of polyethylene, the PTFE membrane is a filtering membrane with the diameter of 50mm, and the effective filtering area is only 20cm ² After sterilization, the possibility of contamination by repeated use is high, and the gas flux is insufficient for large-flux gas supply. Therefore, the utility model discloses improve according to current breather filter's characteristics, provide a big flux air cleaner of removable filter membrane, used repeatedly is not fragile, reduce cost.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a fermentation cylinder filter of ventilating improves according to the characteristics of the filter of ventilating now, provides a big flux air cleaner of removable filter membrane, and used repeatedly is not fragile, reduce cost.

In order to solve the technical problem, the utility model discloses a realize through following technical scheme:

a fermentation tank ventilation filter comprises a first end cover and a second end cover which are overlapped with each other, wherein both the corresponding overlapped sides of the first end cover and the second end cover are provided with a groove body, the center of the other side of the first end cover and the second end cover is connected with a communicating pipe, and the communicating pipe is communicated with the groove body and is used for being sleeved with an air pipe;

the utility model discloses a cell body, including first end cover, second end cover, rubber ring, first end cover and second end cover, first end cover installs the filtration membrane that cuts off cell body and communicating pipe, install the rubber ring in the second end cover, and first end cover and second end cover coincide, rubber ring pressfitting filtration membrane avris is accomplished sealedly.

Furthermore, the avris of first end cover has first limit wall, the avris of second end cover has second limit wall, second limit wall and threaded connection are cup jointed to first limit wall, have the step groove that holds first limit wall between second limit wall and the second end cover limit side.

Further, the second limit wall corresponds and has just seted up the ring channel to one side of first end cover, the rubber ring is the annular ring, and the cross-section is flat, rubber ring one side correspondence has the annular arris of card income ring channel, and the opposite side is used for extruding filtration membrane, makes filtration membrane and the laminating of first end cover.

Furthermore, one side of the rubber ring extrusion filtering membrane is provided with a contraction groove, and the section of the contraction groove is triangular.

Furthermore, the communicating parts of the first end cover and the second end cover and the corresponding communicating pipes are provided with funnel-type flaring designs.

The utility model discloses following beneficial effect has: first end cover and second end cover adopt polyethylene or metal material, and repeatedly practical, first end cover and second end cover can be opened and change filtration membrane, carry out high-pressure steam sterilization simultaneously, and the disinfection is more thorough, and the installation is convenient with the dismantlement, and the leakproofness is good.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used for describing the embodiments will be briefly described below.

FIG. 1: the utility model discloses cross-sectional structure schematic diagram under the mounted state.

FIG. 2: the utility model discloses structural schematic under the split state.

FIG. 3: the utility model discloses figure 2 has the structure sketch map who dissects.

In the drawings, the components represented by the respective reference numerals are listed below: the filter comprises a first end cover 1, a second end cover 2, a communication pipe 3, a filter membrane 4, a rubber ring 5, a first side wall 11, a second side wall 21, an annular groove 22, an annular ridge 51 and a contraction groove 52.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

As shown in fig. 1-3: the utility model provides a fermentation cylinder filter of ventilating, includes first end cover 1 and the second end cover 2 of mutual coincidence installation, and first end cover and second end cover are circular, and first end cover 1 and second end cover 2 correspond the coincidence side and all have the cell body, and the cell body is the circular recess of platykurtic, and the degree of depth is little, and the opposite side central authorities of first end cover 1 and second end cover 2 are connected with communicating pipe 3, have the barb design communicating pipe to dispose the strengthening rib. The communicating pipe 3 is communicated with the groove body and is used for being sleeved with an air pipe;

the first end cover 1 is provided with a filtering membrane 4 for separating the tank body from the communicating pipe, the second end cover 2 is provided with a rubber ring 5, and when the first end cover 1 and the second end cover 2 are superposed, the rubber ring 5 is pressed on the side of the filtering membrane to complete sealing. The filter medium is a PTFE filter membrane, and the hydrophobic polytetrafluoroethylene is a hydrophobic membrane material and can prevent water from flowing through and allow air or gas to filter. First end cover and second end cover adopt polyethylene or metal material, and repeatedly practicality, first end cover and second end cover can be opened and change filtration membrane, carry out high-pressure steam sterilization simultaneously, and the disinfection is more thorough.

The filter membrane can be used repeatedly after replacement, and high temperature and high pressure sterilization is required, and the sterilization is performed by high pressure steam with temperature of 120-130 deg.C for 20min. The ventilating filter is suitable for ventilating devices such as bioreactors, fermentation tanks, incubators and the like. It can also be used for filtering small amount of solvent.

The side of the first end cover 1 is provided with a first side wall 11, the side of the second end cover 2 is provided with a second side wall 21, the first side wall 11 is sleeved on the second side wall 21 and is in threaded connection, and a step groove for accommodating the first side wall is formed between the second side wall 21 and the side of the second end cover 2. After the first side wall is in threaded connection with the second end cover, the first side wall is just accommodated in the step groove, so that the side of the integral filter is in smooth transition without a bulge.

The annular groove 22 is formed in one side, opposite to the first end cover, of the second side wall 21, the rubber ring 5 is an annular ring, the cross section of the rubber ring is flat, one side of the rubber ring 5 is correspondingly provided with an annular ridge 51 clamped into the annular groove 22, and the other side of the rubber ring is used for extruding the filtering membrane 4, so that the filtering membrane 4 is attached to the first end cover. The second end cover is thick, is convenient for set up the ring channel, installs the rubber ring earlier during the installation, and the ring channel is gone into to the annular arris card that the rubber ring passes through integrative design, then first end cover and second end cover coincidence installation, the in-process of screwing, rubber ring pressfitting filtration membrane makes sealed avris.

One side of the rubber ring 5 extruding the filtering membrane 4 is provided with a contraction groove 52, and the section of the contraction groove 52 is triangular. When the rubber ring extrudes the filtering membrane, the rubber ring is further deformed to improve the extrusion sealing performance, and the shrinkage groove provides a rubber ring deformation space.

As shown in fig. 1: the communicating parts of the first end cover 1 and the second end cover 2 and the corresponding communicating pipe 3 are both provided with funnel type flaring designs. The air intake equivalent of the air intake and exhaust port is improved, and the filtering process is accelerated.

The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention.

Claims

1. The utility model provides a fermentation cylinder filter of ventilating which characterized in that:

the gas pipe connector comprises a first end cover (1) and a second end cover (2) which are overlapped and installed with each other, wherein the corresponding overlapped sides of the first end cover (1) and the second end cover (2) are respectively provided with a groove body, the centers of the other sides of the first end cover (1) and the second end cover (2) are connected with a communicating pipe (3), and the communicating pipe (3) is communicated with the groove bodies and is used for being sleeved with a gas pipe;

first end cover (1) is installed and is cut off cell body and communicating pipe filtration membrane (4), install rubber ring (5) in second end cover (2), when first end cover (1) and second end cover (2) coincide, rubber ring (5) pressfitting filtration membrane avris is accomplished sealedly.

2. The fermenter aeration filter of claim 1, wherein: the avris of first end cap (1) has first limit wall (11), the avris of second end cap (2) has second limit wall (21), second limit wall (21) and threaded connection are cup jointed in first limit wall (11), have the step groove that holds first limit wall between second limit wall (21) and second end cap (2) avris.

3. The fermenter aeration filter of claim 2, wherein: annular groove (22) have been seted up just to one side of first end cover to second limit wall (21) correspondence, rubber ring (5) are the annular ring, and the cross-section is flat, rubber ring (5) one side correspondence has annular arris (51) of card income annular groove (22), and the opposite side is used for extrusion filtration membrane (4), makes filtration membrane (4) and first end cover laminating.

4. The fermenter aeration filter of claim 3, wherein: one side of the rubber ring (5) extruding and filtering membrane (4) is provided with a contraction groove (52), and the section of the contraction groove (52) is triangular.

5. The fermenter aeration filter of claim 1, wherein: the communicating parts of the first end cover (1) and the second end cover (2) and the corresponding communicating pipes (3) are both provided with funnel type flaring designs.