CN218035980U - Sample filter equipment and sampling device thereof - Google Patents
Sample filter equipment and sampling device thereof Download PDFInfo
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- CN218035980U CN218035980U CN202221881412.7U CN202221881412U CN218035980U CN 218035980 U CN218035980 U CN 218035980U CN 202221881412 U CN202221881412 U CN 202221881412U CN 218035980 U CN218035980 U CN 218035980U
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Abstract
A sample filter device and sampling equipment thereof are provided, wherein the sample filter device comprises a tube body, a user end cover, an experiment end cover and a filter element; two ends of the tube body are open; the user end cover is used for plugging one end opening of the pipe body; the experimental end cover is used for plugging the opening at the other end of the pipe body; the filter element is positioned in the inner cavity of the tube body, is close to the experiment end cover and is used for filtering a liquid sample in the tube body; the filter element can move towards the user end cover under the action of external force. The filter element is preset in the filter device, and during use, the filter element is not required to be manually placed into the tube body after a sample is added, so that the sample pretreatment step is obviously simplified, and the sample filtration efficiency is improved.
Description
Technical Field
The utility model relates to a sample processing field, concretely relates to sample filter equipment and sampling equipment thereof.
Background
Among the prior art, when carrying out detections such as intestinal cancer, the sample treatment flow is as follows, and excrement sample is collected to the excrement and urine sampling pipe of sampling conventionality, and the back is collected to the sample, mixes on the vortex appearance and evenly vibrates the operation, obtains the excrement and urine supernatant through centrifugation or filterable mode after the mixing. Wherein, the centrifugation mode easily causes the suction head to block and pollute when absorbing the supernatant, thereby influencing the pretreatment efficiency.
The existing device for filtering through the filter element is complex to operate.
SUMMERY OF THE UTILITY MODEL
According to a first aspect, in one embodiment, there is provided a sample filtration device comprising a tube, a user end cap, a laboratory end cap, a filter element;
the two ends of the tube body are open;
the user end cover is used for plugging one end opening of the pipe body;
the experiment end cover is used for plugging the opening at the other end of the pipe body;
the filter element is positioned in the inner cavity of the tube body, is close to the experiment end cover and is used for filtering a liquid sample in the tube body;
the filter element is movable towards the user end cap under an external force.
In one embodiment, a shielding piece is arranged on the tube body and used for shielding the outer side wall of the experiment end cover.
In an embodiment, a clamping piece for clamping a clamp is arranged on the experiment end cover, and the clamp is clamped to the clamping piece, so that the experiment end cover is opened or locked.
In one embodiment, the inner bottom of the user end cap is provided with a protrusion towards the experimental end cap.
In one embodiment, the diameter of the protrusion gradually decreases in a direction toward the experimental end cap.
In one embodiment, the shielding member is provided with at least one through hole.
In one embodiment, the tube body is provided with a stop member for the opening edge of the user end cover to abut against.
In one embodiment, the user end cap and the laboratory end cap are screwed to the pipe body.
In one embodiment, the pore size of the micro-pores of the filter element is 10-500 μm.
In one embodiment, the density of the filter element is more than or equal to 20kg/m 3 。
In one embodiment, the height of the filter element is more than or equal to 5mm.
In one embodiment, the outer surface of the user end cover is provided with first anti-slip lines.
In one embodiment, the outer surface of the experimental end cover is provided with second anti-slip threads.
In one embodiment, a groove is formed in the top of the experiment end cover, and a clamping piece is arranged in the groove.
In one embodiment, the top of the snap is flush with the top of the experimental end cap.
In one embodiment, the pore size of the micro-pores of the filter element is 100-500 μm.
In one embodiment, the density of the filter element is 25-35 kg/m 3 。
In one embodiment, the height of the filter element is 5-20 mm.
In one embodiment, the inner diameter of the tube decreases from the side where the experimental end cap is located toward the side where the user end cap is located.
In one embodiment, the filter element is polyurethane, polyethylene, polypropylene, stainless steel, or ceramic.
In one embodiment, the pore size of the micro-pores of the filter element is 350-400 μm.
In one embodiment, the height of the filter element is 5-10 mm.
According to a second aspect, in an embodiment, there is provided a sampling device comprising the sample filtration apparatus of any one of the first aspects.
According to the sample filtering device and the sampling equipment thereof, the filter element is preset in the filtering device, and when the sample filtering device is used, the filter element does not need to be manually placed into the tube body after a sample is added, so that the sample pretreatment step is obviously simplified, and the sample filtering efficiency is improved.
Drawings
FIG. 1 is a schematic perspective view of a sample filtration device according to an embodiment;
FIG. 2 is a schematic view of a tube with end caps removed from both ends in one embodiment;
FIG. 3 is a schematic view of an embodiment of an outer structure of a user end cap;
FIG. 4 is a schematic view of an inner structure of a user end cap according to an embodiment;
FIG. 5 is a schematic diagram of an exemplary outer side structure of a test end cap;
FIG. 6 is a schematic view of the inner structure of an experimental end cap according to an embodiment;
fig. 7 is a schematic view of a filter element structure according to an embodiment.
Description of reference numerals:
1. a pipe body;
101. a shield;
102. a through hole;
103. a stopper;
2. a user end cap;
201. a first anti-skid pattern;
202. a protrusion;
3. an experimental end cap;
301. a snap-fit member;
302. a second anti-skid pattern;
303. a groove;
304. covering;
4. and (3) a filter element.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted in different instances or may be replaced by other materials, methods. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The ordinal numbers used herein for the components, such as "first," "second," etc., are used merely to distinguish between the objects described, and do not have any sequential or technical meaning. The terms "connected" and "coupled" when used herein, unless otherwise indicated, include both direct and indirect connections (couplings).
The utility model discloses mainly used intestinal cancer etc. detect, but not only restrict this usage.
The current filterable mode then needs to be accomplished with the help of the filter core, uses the filter core after, the suction head blocks up the condition and descends by a wide margin, can effectively get excrement and urine supernatant, but operation process is comparatively complicated, need uncap to add the filter core, also has the risk of pollution, and it is higher with the automatic cost of becoming of filter core scheme simultaneously. In view of the effectiveness but the complexity of the filter element scheme, the sampling device is specially designed, and the filter element is reserved in the sampling pipe, so that the step of uncovering and adding the filter element in the sample processing is omitted. The design of double-end cover has solved the intraductal problem of filter core preset effectively, and especially the cladding structure of body at laboratory end tube cap formation has avoided the problem that the user can uncap the loft wantonly completely, from the source end greatly improve user's sampling operation's uniformity. After this sampling pipe got into the laboratory, through shaking the mixing back, can directly uncap the extrusion filter core and obtain the supernatant to improve excrement and urine sample preliminary treatment efficiency by a wide margin.
For the extraction of the excrement DNA, two extraction strategies are mainly adopted, wherein one is the extraction in the form of excrement suspension liquid, and the other is the extraction in the form of excrement supernatant liquid; corresponding to the two extraction strategies, different pretreatment modes are provided respectively: the pretreatment mode corresponding to the suspension extraction is mixing, but the absorption is difficult and not accurate enough, and the pollution is very easy to cause in the cover-opening absorption process; the pretreatment of the supernatant form is a mode of centrifugation or filtration after uniform mixing, so that invalid residue components settle down, and cells exist in the supernatant, the purity of the DNA extracted by the mode is higher, but the process is more complicated due to the centrifugation or filter element filtration process, and the operation of opening a cover and adding a filter element is involved. In detection processes such as early intestinal cancer screening, solid-liquid separation is carried out on a fecal sample so as to absorb liquid containing relatively less impurities in a supernatant taking process, and the detection precision of the product is improved. The method adopted by the solid-liquid separation of the prior fecal sample mainly comprises the centrifugation of a centrifugal machine or the filtration of a filter element, and the main principle of the centrifugation is that the centrifugal force is generated by rotation to ensure that substances with different densities are settled in liquid at different rates; the main principle of filtration is to separate large-particle substances through the pore size of the filter element.
The existing solid-liquid separation method for detecting the fecal sample in the intestinal cancer comprises the following steps:
the first scheme is as follows: a high speed centrifuge is used. The centrifuge was started by placing the sample in the centrifuge rotor, which was accelerated to 4000 revolutions per minute for 3 minutes. During the high-speed operation of the rotor, fecal impurities in the intestinal cancer fecal sample settle at different rates due to different densities. The overall settling rate increases with increasing material density.
Scheme II: and opening the cover and adding the filter element.
The existing centrifugal equipment used in the solid-liquid separation of intestinal cancer excrement samples mainly comprises a Hunan instrument H2050R high-speed centrifugal machine.
Specification of a sample tube: shenzhen 10mL sampling tube (93 version) produced by Meidike.
The existing solid-liquid separation method for the fecal sample comprises the following specific implementation steps:
(1) And manually placing the uniformly mixed sample tubes containing the mixture of the excrement and the preservation solution into a rotor of the centrifuge in a centrosymmetric manner, and closing an upper cover of the centrifuge.
(2) The set centrifuge program was started at 4000 rpm, running for 3 minutes.
(3) After the program is finished, the upper cover of the centrifuge is opened, and the centrifuged sample tube is taken out manually and placed in a sample box.
The centrifugation method is complex to operate, solid-liquid separation is not thorough, and due to the diversity of human excrement contents, a lot of substances with density smaller than that of preservation solution are contained, and the substances are difficult to settle to the bottom of a sample tube in any way (the samples account for about 35% of the total samples), so that the possibility of liquid taking failure exists in the next step of supernatant taking operation.
The solid-liquid separation of the fecal sample for intestinal cancer detection by using the filter element in the prior art has the following defects:
according to the existing scheme, the cover needs to be opened after the sample is uniformly mixed, the filter element is placed in the sample, one-time cover opening is added in the sample pretreatment link, automation is complex to realize, and meanwhile, the pollution risk is increased.
In one embodiment, a sample storage tube for collecting fecal samples for intestinal cancer detection is provided, the storage tube is designed with double open covers, a filter element is reserved at one end, and after sampling is completed, a laboratory end tube cover (i.e. a laboratory end cover) can be directly opened to absorb supernatant without centrifugation or filter element adding operation.
In one embodiment, as shown in fig. 1, the experimental end of the tube 1 comprises an experimental end cap 3 and a ring of isolation layer (i.e. a covering structure, i.e. a shielding member 101) surrounding the outer side wall of the experimental end cap 3 and having a certain distance from the outer side wall, and the isolation layer is connected to the tube, so that the isolation layer is not unscrewed. The effect of isolation layer is used for distinguishing user's end and laboratory tube cap, prevents that the user from opening experiment end cover 3 because of the maloperation. When needing the manual work to open experiment end cover 3, use the cutter to cut the isolation layer open, wait that experiment end cover 3 exposes the back, can twist experiment end cover 3 through the hand, open this end cover.
In one embodiment, as shown in FIG. 1, a sampling tube for fecal sample collection is provided. The sampling tube is designed to be provided with two open ends and consists of a tube body 1, a user end cover 2, an experiment end cover 3 and a filter element 4. When the sampling pipe is assembled, the filter element 4 is firstly put in from the experimental end, and then the experimental end cover 3 is covered, due to the special structural design of the pipe body 1, a user cannot open the experimental end cover 3 without using a special tool, so that the independence and the stability of the structure and the function of the filter element are ensured; then, the sample preservation solution is added from the user end, and the user end cover 2 is covered to complete the assembly of the sampling tube.
In use, a user opens the user end cap 2 from the user end, adds a sample therefrom, closes the tube cap, and sends it to the testing laboratory. And at the laboratory end, the tube body is turned upside down to the laboratory end and is upwards, and a vortex instrument is used for carrying out uniform mixing operation. After the mixing, open experiment end cover 3 with laboratory instrument (specifically be the automatic anchor clamps of market purchase), the suction head is pressed filter core 4 downwards, and filter core 4 accomplishes and filters, can obtain the supernatant, and this supernatant can directly carry out follow-up extraction operation.
In an embodiment, the utility model discloses a mode at prefabricated filter core in the appearance intraductal has optimized the preliminary treatment link before the excrement and urine sample draws for need not to carry on too much preliminary treatment again after the sample is accomplished, can directly obtain excrement and urine supernatant, greatly improved sample treatment efficiency, also through reducing the link of uncapping, reduced the sample and polluted ground risk simultaneously, improved sample quality.
In an embodiment, the utility model relates to a behind the once die sinking of filter core and tubular product, low cost can be reduced by a wide margin, can reduce automatic research and development input simultaneously, is favorable to promoting the whole profit margin of company.
In one embodiment, as shown in fig. 1 and 2, a sample filtering device is provided, which includes a tube 1, a user end cap 2, an experimental end cap 3, and a filter element 4; two ends of the tube body 1 are open; the user end cover 2 is used for plugging one end opening of the pipe body 1; the experimental end cover 3 is used for plugging the opening at the other end of the tube body 1; the filter element 4 is positioned in the inner cavity of the tube body 1, the filter element 4 is close to the experimental end cover 3, and the filter element 4 is used for filtering liquid in the tube body 1; the filter element 4 can be moved towards the user end cap 2 by an external force. Usually, the filter element 4 is pressed down by a suction head of the sampling device, so that the filter element 4 moves towards the user end cover 2, the liquid sample in the tube body 1 is filtered, and the liquid between the filter element 4 and the experiment end cover 3 is the filtered liquid. The suction head of the sampling device can accurately sample the filtered liquid. The filter element 4 is arranged in the inner cavity of the tube body 1 in advance and is close to the experimental end cover 3, and the filter element 4 is manually placed into the tube body 1 before sample treatment, so that the operation is effectively simplified, and the pollution is reduced.
In one embodiment, the filter element 4 is pre-disposed at a port close to the experimental end cap 3 in an interference fit manner, so that liquid such as preservation liquid pre-filled between the filter element 4 and the user end cap 2 is prevented from overflowing to a side where the experimental end cap 3 is located.
In one embodiment, a proper amount of liquid such as preservation liquid can be filled in the cavity between the filter element 4 and the user end cap 2 in the tube body 1. The preservation solution is mainly used for maintaining the stability of a sample, inhibiting the growth of other microorganisms and maintaining the structural stability of cells and DNA in the cells.
As shown in fig. 1 and 2, a shielding member 101 is provided on the tube body 1 for shielding the outer side wall of the experimental end cap 3. The shield 101 is used to avoid the user opening the test end cap 3 by mistake. The shielding piece 101 is used as a part of the tube body 1 and is fixedly connected with the tube body 1, so that misoperation of a user is effectively avoided.
In one embodiment, the outer wall of the shielding element 101 is smooth, so that the user cannot directly unscrew the shielding element 101 by hand when using the shielding element, thereby further avoiding misoperation.
In an embodiment, the shielding member 101 is enclosed on the outer side wall of the experimental end cap 3, so that the user is effectively prevented from contacting the outer side wall of the experimental end cap 3, and misoperation is avoided.
In one embodiment, as shown in fig. 1, 5 and 6, the experiment end cap 3 is provided with an engaging piece 301 for engaging with a clamp, and the clamp is engaged with the engaging piece 301, so as to open or lock the experiment end cap 3.
The engaging member 301 is shaped to fit the clamp, and in one embodiment, the engaging member 301 may have an outer hexagonal structure for engaging with an inner hexagonal clamp, so as to unscrew the experimental cap 3. In another embodiment, the engaging member 301 may be a cross-shaped mechanism for engaging with a cross-shaped clamp. The shape of the engaging piece 301 is not limited, and may be various shapes to be engaged with the corresponding jig.
In one embodiment, as shown in fig. 4, the inner bottom of the user end cap 2 is provided with a protrusion 202 towards the experimental end cap 3. The diameter of the protrusion 202 gradually decreases in a direction toward the experimental end cap 3. Before opening user's end cover 2, body 1 is usually placed with user's end cover 2 up, but in the in-process of transportation or artificial getting and putting, liquid such as the liquid of preserving of pre-installation in body 1 can splash to the mouth of pipe edge that user's end cover 2 is located because of rocking, and the liquid that splashes to the mouth of pipe edge can follow arch 202 and flow back to the inner chamber of body 1 to avoid along mouth of pipe edge excessive.
In one embodiment, as shown in FIG. 4, the protrusion 202 may be tapered.
In one embodiment, as shown in fig. 1 and 2, the shielding member 101 is provided with at least one through hole 102. If the experiment end cover 3 needs to be manually opened due to the fact that the clamp is lost or equipment faults occur in the clamp used for automatically clamping the clamping piece 301, the shielding piece 101 can be cut from the tube body 1 along the through hole 102 by using a cutter, and after the shielding piece 101 is taken down, the experiment end cover 3 can be unscrewed by holding the outer wall of the experiment end cover 3.
In one embodiment, as shown in fig. 1 and 2, a plurality of spaced through holes 102 may be circumferentially disposed on the shield 101, and the through holes 102 may be flush with or close to the lower edge of the test end cap 103, so as to help the side wall of the test end cap 3 to be substantially the outer wall for the experimenter to hold and open the side wall of the test end cap 3 after opening the shield 101.
In one embodiment, as shown in fig. 1 and 2, the tube 1 is provided with a stopper 103 for the opening edge of the user end cap 2 to abut against. The stop part 103 helps to prevent the user end cap 2 from being excessively screwed towards the experimental end cap 3 to cause thread damage, and on the other hand, the opening edge of the user end cap 2 abuts against the stop part 103 to prevent liquid from overflowing.
In one embodiment, the user end cap 2 and the experimental end cap 3 are screwed to the tube 1. In an embodiment, the both ends lateral wall of body 1 is equipped with the external screw thread, and the inside wall of user end cover 2, experiment end cover 3 is equipped with the internal thread with aforementioned external screw thread assorted for user end cover 2, experiment end cover 3 can the quick shutoff port that corresponds.
In one embodiment, as shown in fig. 1, 3 and 4, the outer surface of the user end cap 2 is provided with a first anti-slip pattern 201.
In one embodiment, as shown in fig. 5 and 6, the outer surface of the experimental end cap 3 is provided with second anti-slip threads 302. The first anti-slip threads 201 and the second anti-slip threads 302 play an anti-slip role, and a user can conveniently hold the outer wall of the end cover by hand and open or screw the end cover.
In an embodiment, the first anti-skid thread 201 and the second anti-skid thread 302 may be a protrusion, a groove, or other shapes. In an embodiment, the first anti-skid thread 201 and the second anti-skid thread 302 can be in various shapes such as a straight line shape and a curved line shape.
In one embodiment, as shown in fig. 5, a groove 303 is formed on the top of the experimental end cap 3, and a locking member 301 is disposed in the groove 303. The groove 303 helps to reduce positional interference of the engaging member 301, and improves compactness.
In an embodiment, as shown in fig. 5, the top of the engaging member 301 is flush with the cap top 304 of the experimental cap 3, so that the tube 1 can be stably placed on a horizontal table when the cap top 304 of the experimental cap 3 faces downward.
In one embodiment, as shown in fig. 1, the inner diameter of the tube 1 gradually decreases from the side where the experimental end cap 3 is located toward the side where the user end cap 2 is located, the filter element 4 has a certain elastic deformation, and the gradually decreasing inner diameter of the tube 1 helps to fully filter the liquid sample in the tube 1, so as to prevent the liquid sample from flowing into the chamber between the filter element 4 and the experimental end cap 3 along the gap between the filter element 4 and the inner wall of the tube 1, without being filtered by the filter element 4.
In one embodiment, the filter element 4 is polyurethane, polyethylene or polypropylene. On one hand, the filter element 4 is used for liquid to pass through, the target cell tissue or nucleic acid passes through the filter element along with the liquid, and detection can be carried out after sampling, and on the other hand, the filter element 4 intercepts impurities in the liquid sample.
In one embodiment, the filter element 4 has a micro-hole for passing a target substance to be separated in the sample liquid, and the filter element is configured to slide along the inner wall of the tube 1 from the experimental end to the user end under the action of an external force to filter the sample liquid.
In one embodiment, the filter element 4 is used to filter impurities in the sample fluid. The particle size of substances (mainly impurities) except the target object is larger than the pore size of the micropores of the filter element and cannot pass through the micropores, and the target object can enter one side of the filter element close to the experimental end from the micropores on one side of the filter element close to the user end, so that the target object is separated, and the sample liquid on one side close to the experimental end hardly contains impurities.
In one embodiment, the target includes, but is not limited to, a cell.
In one embodiment, the cells include, but are not limited to, tumor cells.
In one embodiment, the tumor cells include, but are not limited to, colorectal cancerous tumor cells.
In one embodiment, the sample fluid includes, but is not limited to, a fecal sample fluid.
In one embodiment, the maximum diameter of the filter element is not larger than the inner diameter of the container for holding the sample liquid, or slightly larger than the inner diameter of the container. The filter element usually has a certain deformation, so that the outer wall of the filter element is tightly attached to the inner wall of the container, and sample liquid is prevented from flowing into the upper part of the filter element from a gap between the filter element and the inner wall of the container without being filtered by the filter element.
In one embodiment, the pore size of the pores of the filter element 4 is 10 to 500. Mu.m, preferably 100 to 500. Mu.m, more preferably 300 to 500. Mu.m. The pore size of the pores of the filter element includes, but is not limited to, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 60 μm, 70 μm, 80 μm, 90 μm, 100 μm, 150 μm, 200 μm, 250 μm, 300 μm, 350 μm, 450 μm, 500 μm.
If the pore diameter of the micropore is too small, target cell tissues or nucleic acid are difficult to permeate the filter element, so that the detection sensitivity of an experimental result is reduced; if the aperture is too big, then large granule impurity can see through the filter core, arouses the sample link suction head to block up and then leads to the sample failure.
In one embodiment, the density of the filter element 4 is more than or equal to 20kg/m 3 Preferably 25 to 35kg/m 3 More preferably 30kg/m 3 . The density of the filter element includes, but is not limited to, 25kg/m 3 、26kg/m 3 、27kg/m 3 、28kg/m 3 、29kg/m 3 、30kg/m 3 、31kg/m 3 、32kg/m 3 、33kg/m 3 、34kg/m 3 、25kg/m 3 。
In one embodiment, the filter element 4 is polyurethane, polyethylene, polypropylene, stainless steel or ceramic, and the material used to make the filter element is conventional and commercially available.
If the density of the filter element is too low, the filter element can float upwards and is difficult to take and place; if the density is too high, the radial elasticity of the filter cartridge will be reduced, possibly making it difficult for the filter cartridge to be pushed by the suction head into the user end of the tubular body 1.
In one embodiment, the sample added to the tube 1 is a liquid sample.
In one embodiment, as shown in FIG. 7, the cartridge 4 has a cylindrical portion.
The shape of filter core 4 is unrestricted, can be various shapes such as cylinder, cone, can make the inner wall that body 1 was hugged closely to the filter core slide to the user side, and the various shapes of filtering sample liquid all are applicable to the utility model discloses. A1 inner chamber of body for holding sample liquid is cylindrical usually, and consequently, the filter core is preferably the cylinder or the cylinder of bottom for the back taper, can effectively avoid the filter core to take place the skew in sample liquid to avoid causing the filtration incomplete.
In one embodiment, the height of the filter element is greater than or equal to 5mm, preferably 5-20 mm, and more preferably 5-10 mm.
In one embodiment, the diameter of the filter element is greater than or equal to 5mm, preferably 5-30 mm, and more preferably 10-20 mm.
In one embodiment, the diameter, height, etc. of the filter element are not limited, and can be designed according to actual requirements. The diameter of the filter element is slightly larger than the inner diameter of the pipe body 1, so that interference fit is realized.
In an embodiment, there is provided a sampling device comprising the sample filtration apparatus of any of the preceding embodiments. In one embodiment, the sampling device can be an automatic sampling device, which automatically controls the suction head to press down the filter element 4, completes the filtering, and then automatically and accurately samples for subsequent detection.
During the use, the one end at user end cover 2 place is earlier up, and the user opens user end cover 2 back, adds the liquid sample in to the inner chamber of body 1, then screws up user end cover 2, reverses body 1 to experiment end cover 3 up, uses the vortex appearance to carry out the mixing operation. After the mixing, open experiment end cover 3 with laboratory instrument (for example, the automatic anchor clamps of purchase), the suction head is pressed filter core 4 downwards, and filter core 4 accomplishes and filters, and the liquid that obtains between filter core 4 and the experiment end cover 3 is the liquid after through filter core 4 filtration, and the supernatant is, and this supernatant can directly carry out follow-up extraction operation.
In an embodiment, solid-liquid separation of the intestinal cancer detection excrement sample is realized by the filter element 4, and compared with a solid-liquid separation mode adopted by the existing centrifuge product, the solid-liquid separation efficiency of the filter element can be greatly improved, and substances with the density smaller than that of the preservation solution in the filtered sample solution are remarkably reduced.
Example 1
The sampling pipes shown in the figures 1 to 7 are compared with the existing centrifugation and manual filter element adding methods to carry out sample pretreatment detection, and the detection results are compared.
The material of the filter element is high-density polyurethane with the density of 30kg/m 3 . The polyurethane used to prepare the filter element in this example was purchased from san jowar, sanderi environmental materials, inc.
The filter element has a micropore diameter of 375 μm, a diameter of 14.2mm and a height of 10mm.
In this embodiment, the structure of body 1 of sampling pipe is cylindric, and the external diameter size of body 1 is 21mm, and highly is 95.57mm.
Group one: the results of sample collection and supernatant collection using the sampling tubes shown in FIGS. 1 to 7 are shown in Table 1.
TABLE 1
And a second group: the supernatant was taken by conventional tube sampling + centrifugation and the results are shown in Table 2.
TABLE 2
And (3) group III: the supernatant was obtained by filtration through a conventional sampling tube plus a filter cartridge, and the results are shown in Table 3.
TABLE 3
The conventional sampling tubes in the group two and the group three are 10mL sampling tubes (93) produced by commercial Shenzhen Medidaceae, and are of single-opening and single-end cap structures. And in the third group, the tube cover is opened, after sample adding, the filter element is manually placed into the inner cavity of the tube body from the sample adding port, the filter element is pressed downwards by the suction head, and then filtered supernatant is taken.
The results were analyzed as follows:
1. selection of a pretreatment mode: the success rate of the filter element mode is obviously higher than that of the centrifugal mode.
2. Use the utility model discloses a sampling pipe carries out excrement and urine sample collection and preliminary treatment gained testing result and uses uncap to add the filter core preliminary treatment mode and carry out the result identical that detects, but operating time and process are showing and are being less than the latter. Therefore, the utility model discloses under the prerequisite that does not influence the testing result, greatly improved excrement and urine sample pretreatment efficiency, saved a large amount of manpowers and time.
In an embodiment, the utility model discloses take the bi-polar design of uncapping, laboratory end and user side are independent to be separated, mutual noninterference, the filter core is reserved to the laboratory end to make the user can't open the laboratory end mouth of pipe under the condition with the help of the instrument through special body structure, guarantee the filter core and the integrality of function, the user accomplishes excrement and urine sample collection back through the user side, need not complicated operation and can obtain the supernatant that can directly be used for extracting rapidly high-efficiently, greatly improve excrement and urine sample preliminary treatment efficiency.
In one embodiment, the present invention provides a sampling tube for fecal sample preservation and pretreatment.
In one embodiment, the shape of the pipe body 1 is not limited, and the pipe body 1 having a cylindrical shape, a circular truncated cone shape, a rectangular parallelepiped shape, or the like is within the scope of the present invention.
In an embodiment, the size of the pipe body 1 is not limited, and may be designed as required, for example, the outer diameter of the pipe body 1 may be 10mm to 30mm, and the height may be 10mm to 200mm.
In one embodiment, the material of the filter element 4 may include, but is not limited to, any one of the existing materials such as polyurethane, polyethylene, polypropylene, stainless steel, and ceramic.
It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.
Claims (12)
1. A sample filtering device is characterized by comprising a pipe body (1), a user end cover (2), an experiment end cover (3) and a filter element (4);
two ends of the pipe body (1) are open;
the user end cover (2) is used for plugging one end opening of the pipe body (1);
the experiment end cover (3) is used for plugging the opening at the other end of the pipe body (1);
the filter element (4) is positioned in the inner cavity of the tube body (1), the filter element (4) is close to the experiment end cover (3), and the filter element (4) is used for filtering a liquid sample in the tube body (1);
the filter element (4) can move towards the user end cover (2) under the action of external force.
2. The sample filtration device according to claim 1, wherein the tubular body (1) is provided with a shield (101) for shielding the outer side wall of the experimental end cap (3).
3. The sample filtration device according to claim 1, wherein the laboratory end cap (3) is provided with a snap (301) for a clip to snap to the snap (301) to open or lock the laboratory end cap (3).
4. The sample filtration device as claimed in claim 1, wherein the inner bottom of the user end cap (2) is provided with a protrusion (202) towards the laboratory end cap (3).
5. The sample filtration device according to claim 4, wherein the diameter of the bulge (202) is gradually decreasing in a direction towards the laboratory end cap (3).
6. The sample filtration device according to claim 2, wherein the shutter (101) is provided with at least one through hole (102).
7. The sample filtration device according to claim 1, wherein the tube (1) is provided with a stop (103) against which an opening edge of the user end cap (2) abuts;
the user end cover (2) and the experiment end cover (3) are in threaded connection with the pipe body (1).
8. The sample filtration device as claimed in claim 1, wherein the outer surface of the user end cap (2) is provided with a first anti-slip texture (201);
the outer surface of the experiment end cover (3) is provided with second anti-skid grains (302);
a groove (303) is formed in the top of the experiment end cover (3), and a clamping piece (301) is arranged in the groove (303);
the top of the clamping piece (301) is flush with the top (304) of the experimental end cover (3).
9. The sample filtration device according to claim 1, wherein the inner diameter of the tubular body (1) decreases from the side where the laboratory end cap (3) is located towards the side where the user end cap (2) is located;
the filter element (4) is made of polyurethane, polyethylene, polypropylene, stainless steel or ceramic.
10. The sample filtration device according to claim 1, wherein the filter element (4) has a pore size of 10 to 500 μm;
the density of the filter element (4) is more than or equal to 20kg/m 3 。
11. The sample filtration device according to claim 1, wherein the filter element (4) has a pore size of the micropores of between 100 and 500 μm;
the density of the filter element (4) is 25-35 kg/m 3 。
12. A sampling device comprising the sample filtration apparatus of any one of claims 1 to 11.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221881412.7U CN218035980U (en) | 2022-07-20 | 2022-07-20 | Sample filter equipment and sampling device thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221881412.7U CN218035980U (en) | 2022-07-20 | 2022-07-20 | Sample filter equipment and sampling device thereof |
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| Publication Number | Publication Date |
|---|---|
| CN218035980U true CN218035980U (en) | 2022-12-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221881412.7U Active CN218035980U (en) | 2022-07-20 | 2022-07-20 | Sample filter equipment and sampling device thereof |
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| Country | Link |
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| CN (1) | CN218035980U (en) |
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