CN114832471A - Filter element and filtering device and method thereof - Google Patents
Filter element and filtering device and method thereof Download PDFInfo
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- CN114832471A CN114832471A CN202210555835.8A CN202210555835A CN114832471A CN 114832471 A CN114832471 A CN 114832471A CN 202210555835 A CN202210555835 A CN 202210555835A CN 114832471 A CN114832471 A CN 114832471A
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/11—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
- B01D29/31—Self-supporting filtering elements
- B01D29/35—Self-supporting filtering elements arranged for outward flow filtration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/01—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons
Abstract
A filter element and a filtering device and method thereof, the filter element is provided with micropores for the target substance to be separated in sample liquid to pass through, and the pore diameter of the micropores of the filter element is 10-500 mu m. The filter element can obviously reduce the substance residue with density less than that of the preservation solution in the sample solution, and obtain the high-purity filtered sample solution, thereby obviously reducing the abnormal rate of the sample detection result.
Description
Technical Field
The invention relates to the field of sample collection devices and methods, in particular to a filter element, a filter device and a method thereof.
Background
In early screening products of intestinal cancer, solid-liquid separation is carried out on a fecal sample in order to absorb liquid containing relatively few impurities in the process of taking supernate, and the detection accuracy of the products is improved. The main product used for solid-liquid separation of the existing fecal samples is a centrifuge, which is an electrical device, and the main principle is that substances with different densities are settled in liquid at different rates by generating centrifugal force through rotation.
The current intestinal cancer detects excrement and urine sample solid-liquid separation product includes:
a high-speed centrifuge. The centrifuge was started by placing the sample in the centrifuge rotor, which was accelerated to 4000 revolutions per minute for 3 minutes. During the high-speed operation of the rotor, fecal impurities in the intestinal cancer fecal sample settle at different rates due to different densities. The overall settling rate increases with increasing material density.
Products used by the existing intestinal cancer excrement sample solid-liquid separation technology are mainly a sample tube and a centrifuge, such as a Hunan instrument H2050R high-speed centrifuge.
The concrete implementation steps of the existing solid-liquid separation method for the fecal sample comprise:
and manually placing the uniformly mixed sample tubes containing the mixture of the excrement and the preservation solution into a rotor of the centrifuge in a centrosymmetric manner, and closing an upper cover of the centrifuge.
The set centrifuge program was started at 4000rpm, running for 3 minutes.
And after the program is finished, opening the upper cover of the centrifuge, and manually taking out the centrifuged sample tube and placing the centrifuged sample tube into a sample box.
The solid-liquid separation of the fecal sample for intestinal cancer detection by the existing centrifugal machine has the following defects:
the solid-liquid separation is not thorough. Due to the diversity of human excrement contents, which contain many substances with density less than that of the preservation solution, the substances are difficult to settle to the bottom of the sample tube (the samples account for about 35% of the total samples) in any centrifugation process, so that the supernatant taking operation in the next step has the possibility of liquid taking failure.
Disclosure of Invention
According to a first aspect, in one embodiment, there is provided a filter element having micropores through which a target substance to be separated in a sample liquid passes, the pore size of the micropores of the filter element being 10 to 500 μm.
According to a second aspect, an embodiment provides a filtering apparatus, comprising a filter element and a container for containing a sample liquid, wherein the filter element has micropores for passing a target substance to be separated in the sample liquid, and the filter element is used for sliding along the inner wall of the container to filter the sample liquid.
According to a third aspect, there is provided in one embodiment a method of filtering, comprising: putting the filter element of the first aspect into a container containing sample liquid, pressing the filter element downwards, allowing the sample liquid below the filter element in the container to enter the filter element from the lower surface of the filter element, flow through the micropores of the filter element, and then reach the upper part of the filter element, wherein the liquid above the filter element is the filtered sample liquid.
According to the filter element, the filter device and the method thereof, the filter element can obviously reduce the substance residue with density lower than that of the preservation solution in the sample solution, and obtain the high-purity filtered sample solution, thereby obviously reducing the abnormal rate of the sample detection result.
Drawings
FIG. 1 is a schematic view of a filter cartridge arrangement according to one embodiment;
FIG. 2 is a front view of a filter cartridge according to one embodiment;
FIG. 3 is a top view of a filter cartridge according to one embodiment;
FIG. 4 is a schematic view of a sampling tube according to an embodiment.
Description of reference numerals: 1. a filter element; 11. a cylindrical portion; 12. a lower surface; 13. an upper surface; 2. a pipe body; 21. a pipe orifice; 22. and (7) covering the tube.
Detailed Description
The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning.
As used herein, a "sampling tube" is a tubular means for holding and/or transporting a biological sample, which can typically be placed on a centrifuge apparatus for centrifugation. Herein, "sampling tube" and "sample tube" are used interchangeably.
According to a first aspect, in one embodiment, a filter element is provided, the filter element having micropores for passing a target substance to be separated in a sample liquid, and the pore size of the micropores of the filter element is 10 to 500 μm. The filter element can be sold as a separate product. The pore diameter of the micropores of the filter element is preferably 100 to 500 μm, more preferably 300 to 500 μm, more preferably 350 to 400 μm, and more preferably 375 μm. The pore size of the pores of the filter element includes, but is not limited to, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 60 μm, 70 μm, 80 μm, 90 μm, 100 μm, 125 μm, 150 μm, 175 μm, 200 μm, 225 μm, 250 μm, 275 μm, 300 μm, 325 μm, 350 μm, 375 μm, 450 μm, 475 μm, 500 μm.
In one embodiment, the material of the filter element includes, but is not limited to, polyurethane, polypropylene, or polyethylene, preferably polyurethane.
In one embodiment, the density of the filter element is more than or equal to 20kg/m 3 Preferably 25 to 35kg/m 3 More preferably 30kg/m 3 . The density of the filter element includes, but is not limited to, 20kg/m 3 、21kg/m 3 、22kg/m 3 、23kg/m 3 、24kg/m 3 、25kg/m 3 、26kg/m 3 、27kg/m 3 、28kg/m 3 、29kg/m 3 、30kg/m 3 、31kg/m 3 、32kg/m 3 、33kg/m 3 、34kg/m 3 、35kg/m 3 。
The filter element density is too low, so that the filter element can float upwards and is difficult to take and place; if the density is too high, the radial elasticity of the filter element is reduced, which may make it difficult for the filter element to be pushed into the bottom of the sampling tube by the suction head.
In one embodiment, the target includes, but is not limited to, a cell.
In one embodiment, the cells include, but are not limited to, tumor cells.
In one embodiment, the tumor cells include, but are not limited to, colorectal cancerous tumor cells.
In one embodiment, the sample fluid includes, but is not limited to, a fecal sample fluid.
In one embodiment, the maximum diameter of the filter element is not greater than the inner diameter of the container for holding the sample liquid, or slightly greater than the inner diameter of the container.
In one embodiment, the filter element is cylindrical.
In one embodiment, the height of the filter element is greater than or equal to 5mm, preferably 5-20 mm, and more preferably 5-10 mm.
In one embodiment, the diameter of the filter element is greater than or equal to 5mm, preferably 5-30 mm, and more preferably 10-20 mm.
According to a second aspect, in an embodiment, a filtering device comprises the filter element of any one of the first aspect and a container for containing a sample liquid, wherein the filter element has micropores for passing a target substance to be separated in the sample liquid, and the filter element is used for sliding along the inner wall of the container to filter the sample liquid.
In one embodiment, the filter element is used to filter impurities in a sample fluid. The particle size of the substances (mainly impurities) except the target object is larger than the pore size of the micropores of the filter element, the substances cannot pass through the micropores, the target object can enter the other side of the filter element from the micropores on one side of the filter element, so that the target object is separated, and the sample liquid on the other side hardly contains impurities.
In one embodiment, at least a portion of the outer wall of the filter element can be attached to and slide along the inner wall of the container holding the sample fluid.
In one embodiment, the pore diameter of the micro-pores of the filter element is 10-500 μm, preferably 100-500 μm, and more preferably 300-500 μm. The pore size of the pores of the filter element includes, but is not limited to, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 60 μm, 70 μm, 80 μm, 90 μm, 100 μm, 150 μm, 200 μm, 250 μm, 300 μm, 350 μm, 450 μm, 500 μm.
If the pore diameter of the micropores is too small, target cell tissues or nucleic acid are difficult to permeate through the filter element, so that the detection sensitivity of an experimental result is reduced; if the aperture is too big, then large granule impurity can see through the filter core, arouses the sample link suction head to block up and then leads to the sample failure.
In one embodiment, the material of the filter element includes, but is not limited to, polyurethane, polypropylene, polyethylene, stainless steel, or ceramic.
In one embodiment, the density of the filter element is more than or equal to 20kg/m 3 Preferably 25 to 35kg/m 3 More preferably 30kg/m 3 . The density of the filter element includes, but is not limited to, 25kg/m 3 、26kg/m 3 、27kg/m 3 、28kg/m 3 、29kg/m 3 、30kg/m 3 、31kg/m 3 、32kg/m 3 、33kg/m 3 、34kg/m 3 、25kg/m 3 。
If the density of the filter element is too low, the filter element can float upwards and is difficult to take and place; if the density is too high, the radial elasticity of the filter cartridge will be reduced, possibly making it difficult for the filter cartridge to be pushed by the suction head into the bottom of the sampling tube.
In one embodiment, the cartridge has a cylindrical portion. The shape of the filter element is not limited, and the filter element can be in various shapes such as a cylinder, a cone and the like, so that the filter element can slide down along the inner wall of the container in a manner of being tightly attached to the inner wall of the container, and various shapes of filtered sample liquid are suitable for the invention. A container inner chamber for holding sample liquid is cylindrical usually, and consequently, the filter core is preferably the cylinder or the cylinder that the bottom is the back taper, can effectively avoid the filter core to take place the skew in sample liquid, causes to filter incompletely.
In one embodiment, the target includes, but is not limited to, a cell.
In one embodiment, the cells include, but are not limited to, tumor cells.
In one embodiment, the tumor cells include, but are not limited to, colorectal cancerous tumor cells.
In one embodiment, the sample fluid includes, but is not limited to, a fecal sample fluid.
In one embodiment, the maximum diameter of the filter element is not greater than the inner diameter of the container for holding the sample liquid, or slightly greater than the inner diameter of the container. The filter element usually has certain deformation, makes the outer wall of the filter element cling to the inner wall of the container, and prevents sample liquid from flowing into the upper part of the filter element from the gap between the filter element and the inner wall of the container without being filtered by the filter element.
In one embodiment, the container for holding the sample liquid may be any existing container, and may be a sampling tube, and the shape, specification, model and manufacturer of the sampling tube are not limited, and any sampling tube is suitable for the present invention.
According to a third aspect, in an embodiment, there is provided a filtering method comprising: the filter element of any one of the first aspect is placed in a container containing sample liquid, the filter element is pressed downwards, the sample liquid positioned below the filter element in the container enters the filter element from the lower surface of the filter element, flows through the micropores of the filter element and then reaches the upper part of the filter element, and the liquid above the filter element is the filtered sample liquid.
In one embodiment, the method further comprises drawing a desired sample fluid from the fluid above the filter element.
In one embodiment, the control system controls the sampler to draw a desired sample liquid from the liquid above the filter element.
In one embodiment, the control system controls the filter element to enter the container containing the sample liquid, the filter element is pressed downwards, the sample liquid in the container below the filter element permeates from the lower surface of the filter element, flows through the micropores of the filter element and then reaches the upper part of the filter element, and the liquid above the filter element is the filtered sample liquid.
In one embodiment, the control system includes, but is not limited to, an embedded system, a Programmable Logic Controller (PLC), and the like.
In an embodiment, the invention provides a filter element for realizing solid-liquid separation of an excrement sample for intestinal cancer detection, compared with a solid-liquid separation mode adopted by the existing centrifuge product, the filter element can greatly improve the solid-liquid separation efficiency, and substances with the density smaller than that of a preservation solution in a filtered sample solution are obviously reduced.
In one embodiment, the filter element structure is mainly developed aiming at an automatic application scene, and large-particle impurities can be filtered by automatically grabbing the filter element and putting the filter element into a sampling pipe.
In the prior art, some filter elements are provided with multiple pores for fully mixing a sample and a preservation solution, and the filter element disclosed by the invention is of a porous structure, can filter large-particle impurities and is suitable for an automatic sampling scene.
Example 1
As shown in fig. 1, which is a schematic perspective view of a filter cartridge, the filter cartridge 1 is a cylinder, and has a cylindrical portion 11, a lower surface 12, and an upper surface 13.
Fig. 2 shows a front view of the filter element, and the height h of the filter element 1 can be 6-10 mm, which is only an exemplary example, and can be other heights.
Fig. 3 shows a top view of the filter element, the diameter d of the filter element 1 can be 10-15 mm.
Fig. 4 shows that sampling tube structure sketch map, body 2 have cylindrical inner chamber, are equipped with sample liquid in the inner chamber, and the top of body 2 is equipped with mouth of pipe 21 and is used for the tube cap 22 of the tight mouth of pipe 21 of lid, takes out behind the tube cap 22, puts into the inner chamber of body 2 with filter core 1 from mouth of pipe 21, and the inner wall to sampling tube 1 is hugged closely to the lateral wall of column portion 11, and handheld pipettor of pricking the suction head supports the filter core into the sampling tube bottom easily. The sample liquid enters the filter element from the lower surface 12 of the filter element 1 and then flows out from the upper surface 13, the liquid above the filter element 1 is the filtered sample liquid, and the structure and the corresponding filtering method obviously reduce the substance residue with the density smaller than the preservation liquid, thereby obviously improving the separation effect.
In this embodiment, a 10mL sampling tube (93) manufactured by meidcao biomedical science and technology ltd, shenzhen, is used for the subsequent filter element testing experiment. Without being limited thereto, the filter element of the present invention is applicable to any sampling tube.
Based on the drawing of the intestinal cancer sampling tube size, intestinal cancer tumor unicells and the tissue cell diameter, a filter structure is designed, and the structure has the following characteristics:
1. tumor cells and normal cells in the excrement are easy to permeate, and excrement impurities (mainly food residues) larger than or equal to the caliber of the suction head are not easy to permeate; the diameter of a single colorectal cancer tumor cell is about 10 mu m, a human intestinal exfoliated cell is generally a tissue formed by multiple cells, and the aperture of the filter element designed by the embodiment is larger than 10 mu m, so that the filter element can be smoothly penetrated by the tumor cell and normal cells in excrement.
2. This structure is easily put into the sampling pipe through manual work or automatic mode to realize easily that the filtration action is accomplished from the vertical removal of mouth of pipe to the socle.
3. The structure is simple, the production is easy, and the cost is low.
4. The caliber of a conventional 1000-microliter manual suction head is 500 micrometers, and the caliber of an automatic 1000-microliter suction head is 1000 micrometers; the diameter of the filter hole of the filter element is 10-500 micrometers, and the diameter of the filter hole is less than or equal to the caliber of the suction head, so that the suction head can sample filtered liquid conveniently.
5. The filter element of the embodiment can bear deformation to a certain degree, and is suitable for a sampling pipe cavity structure with an inverted boss with a large caliber and a gradually narrowed bottom.
6. The production process of the filter element material can select foaming, weaving, stamping, injection molding and the like, the manufacturing cost is sequentially foaming, stamping, weaving and injection molding from low to high, and the foaming material with the lowest cost is selected in the embodiment.
The filter core structure that this embodiment designed is the cylinder, designs not unidimensional, and whether the test meets the requirements, and the test sample is as follows:
TABLE 1 Filter element Specification Table
The density of the third and fourth batches of polyurethane was 20kg/m 3 The density of the fifth and sixth batches of high-density polyurethane is 30kg/m 3 。
The test experiment was performed as follows (only if the previous test passed the next test):
the first step of the test experiment is to test the filter element passing performance in the sampling pipe.
The test method comprises the following steps: and horizontally placing the filter element at the opening of a sampling pipe filled with a liquid sample, if the pipette which is held by a hand and is tied with a suction head can easily push the filter element into the bottom of the sampling pipe, judging the filter element to be qualified, and otherwise, judging the filter element to be unqualified. The step is the suitability test of the filter element and the sampling pipe.
And in the second step of test experiment, the filter element is tested for passing through the clear liquid in the excrement sample.
The test method comprises the following steps: and (3) pushing the filter element into a liquid sample of the sampling pipe, and observing the passing property of the liquid in the filter element, wherein if the clear liquid can completely pass within 1s, the passing property is qualified. Otherwise, if the filter element floats upwards to cause that the clear liquid is difficult to completely pass through the filter element, the filter element is judged to be unqualified.
And thirdly, testing the influence of the filter element on the final detection result of the intestinal cancer.
The test method comprises the following steps: the 540 samples were divided into three groups, namely a control group, an experimental group 1 and an experimental group 2, and 180 samples in each group were obtained. The samples are subpackaged in sampling tubes, the total volume of each sample is 5mL, and the solid volume of each sample is 1.5 mL. The centrifuge specification and parameters were set as follows: hunan apparatus H2050R, 4000rpm, 3 min. A liquid transfer device: an Ebende 1000. mu.L manual pipette. Automated sampling apparatus: huada zhi MGISTP-7000 automatic cup separating system.
The description of each group is as follows:
control group: without a filter element, centrifugation was performed, and the filtered supernatant was removed by a manual pipette.
Experimental group 1: adding a filter element, centrifuging, and automatically taking supernate.
Experimental group 2: adding a filter element, and automatically taking supernate without centrifugation.
And setting a control group, an experimental group 1 and an experimental group 2 according to the rules to perform a control experiment, and taking the final QPCR off-line data and the final result consistency rate of data analysis as a judgment index. The qualification criteria are as follows: in the data comparative analysis result of the experimental group 1 or the experimental group 2 and the control group, the strong positive consistency rate is more than or equal to 90%, the weak positive consistency rate is more than or equal to 75%, and the negative consistency rate is more than or equal to 90%.
TABLE 2 Filter test results
The first step test results:
the filter element is made of stainless steel, ceramic or polyethylene, and the filter element is unqualified in the first step. The test results of polypropylene, polyurethane and high-density polyurethane are qualified.
The second step of test results:
the filter core is made of polypropylene, polyurethane and high-density polyurethane, and the test result is qualified.
The third step is to test the result:
according to the test result, the qualified filter element specification is the high-density polyurethane filter element with the cylinder, the diameter of 14.2mm, the height of 10mm and the pore diameter of 375 mu m, and the filter element can be used as a filter material for subsequent experiments to carry out repeated test experiments.
The test experiments were repeated as follows:
the samples were subjected to two replicate experiments. First experiment of the sample: the original group is without filter element, centrifugation, manual sampling. The second experiment of the sample was divided into three groups, each group was treated as follows:
1) the first group was filter-cartridge filtered and then supernatant was taken automatically using MGISTP-7000.
2) The second group is that the filter element is used for filtration, then the centrifuge is used for centrifugation, and then the MGISTP-7000 is used for automatic supernatant taking.
3) The third group is that without filter element, centrifuge centrifuges, then the supernatant is taken manually.
The specific steps of filtering and subsequent automated sampling using a filter cartridge are as follows:
(1) and taking the sampling tube out of the sample box, and opening the tube cover.
(2) The tweezers are adopted to horizontally clamp the cylindrical surface at the position of 0-6 mm of the upper end surface of the filter element, the filter element is horizontally placed into the pipe orifice, the sinking depth is about 2-3 mm, and the tweezers do not contact the pipe orifice during operation.
(3) And covering a sampling pipe cap, screwing down, and completely propping the filter element into the sampling pipe.
(4) And placing the sampling pipe added with the filter element on a carrier of MGISTP-7000, and selecting corresponding parameters to start the equipment.
(5) And after the equipment is operated, obtaining a sample which is subjected to solid-liquid separation and takes 1mL of supernatant and is positioned in the deep-hole plate.
The intestinal cancer complete detection experiment of the filter element is subjected to sample repeatability tests of 800 cases, and the results are contrasted as follows:
TABLE 3 Filter element test results
As can be seen from Table 3, with the second group as a reference, the strong-positive coincidence rate, the weak-positive coincidence rate and the positive coincidence rate of the first group are all higher than those of the third group, which indicates that the automatic sampling improves the detection accuracy after the filter element is added.
The embodiment provides a filter core for solid-liquid separation of an intestinal cancer detection excrement sample. The specific information of the filter element is as follows:
the main materials are as follows: polyurethanes (polymers containing-NHCO-groups in the backbone are collectively referred to as polyurethane urethanes, simply polyurethanes). Specifically, the polyurethane is prepared by using polyester polyol (the mass ratio of the polyester polyol to the total raw materials is about 40%) and toluene diisocyanate (the mass ratio of the toluene diisocyanate to the total raw materials is about 35%) as main raw materials, and the rest 25% of the polyurethane is various auxiliary materials, including a tertiary ammonium compound, a foam stabilizer, water, a pigment, a tin catalyst and the like. The polyurethane used to prepare the filter element in this example was purchased from san jowar, sanderi environmental materials, inc.
The manufacturing process comprises the following steps: one-step foaming method and die cutting.
Material density: 30kg/m 3 (i.e., high density polyurethane).
Pore diameter of the micropores: 375 μm.
The applicable scene of this embodiment is intestinal cancer detection, carries out the solid-liquid separation before the branch cup to the excrement and urine sample that contains the preservative fluid, and the sample pipe that the filter core of this embodiment is suitable for is Shenzhen Meidike 10mL sampling tube-93 money. For sample tubes produced by other manufacturers, the filter element can be adapted by adjusting the shape and the size of the filter element. The sample tube can slide downwards along the inner wall of the sample tube under the micro deformation, so that the sample liquid is filtered.
The filter element realizes solid-liquid separation by the following steps:
1. in the biosafety cabinet, an experimenter takes out 1 sample tube containing the uniformly mixed fecal sample from the sample box and vertically places the sample tube on an operation table of the biosafety cabinet.
2. An experimenter holds the transparent bottle body of the 10mL sampling tube with one hand and keeps the bottom of the sampling tube horizontal, holds the bottle cap of the 10mL sampling tube with the other hand, and rotates the bottle cap counterclockwise for multiple times until the bottle cap is unscrewed, so that the unscrewed sampling tube cap is horizontally inverted on an operation table of a biological safety cabinet.
3. The hand holding the bottle body is kept still, the other hand takes the tweezers to clamp the cylindrical surface of the filter element (cylinder, outer diameter 14.2mm, height 10mm, material high-density polyurethane, foaming process), the height of the upper end surface of the filter element is not more than 5mm, the filter element is horizontally placed into the opening of the sampling tube, and the placement depth is that the distance between the lower section of the filter element and the opening of the sampling tube is more than 1mm and less than 3 mm.
4. After the tweezers are placed at the corresponding positions, the cover inverted on the operation table board of the biological safety cabinet is taken up, after the wrist is rotated to be placed right, the pipe cover is covered, meanwhile, the filter element protrudes out of the pipe orifice part of the sampling pipe and is abutted into the sampling pipe, and the pipe cover is rotated clockwise until the pipe cover is screwed down.
5. All sampling tubes were removed and placed on the carriage of an MGISTP-7000 automated cup dispensing apparatus manufactured by huada.
6. Selecting the type of the sampling pipe, the position of the sampling pipe, the type of the suction head, the position of the suction head, the type of the deep hole plate and the position of the deep hole plate on an interface of an upper computer, closing a window and starting a cup dividing program.
7. And after the equipment is operated, opening the equipment window and taking out the deep hole plate.
And finally, completely completing the solid-liquid separation of the fecal sample for intestinal cancer detection and finishing the supernatant taking operation.
In one embodiment, the filter element is arranged in the sampling pipe in a preposed mode, so that the problem of incomplete solid-liquid separation in the conventional centrifugal mode is solved, and the conversion of the one-time sampling abnormal rate of the intestinal cancer detection excrement sample from more than 35% to less than 1% is realized.
In one embodiment, the invention eliminates the problem that the conventional automatic cup separating system MGISTP-7000 can not avoid the blockage of the suction head by the suspended matters on the liquid surface of the excrement sample, successfully realizes the automation of the supernatant taking link, and improves the supernatant taking efficiency by 100 percent.
In one embodiment, the sampling tube taking and placing device is simple to operate, a centrifugation link adopted at present is omitted, the action that the sampling tube needs to be taken and placed frequently by manpower in a centrifugation mode is reduced, and the labor intensity of personnel is greatly reduced.
In one embodiment, the filter element used in the invention has low cost and easy acquisition, can greatly improve the quality and efficiency of material exchange with low cost, and is beneficial to reducing the sample liquid treatment cost.
The following table shows the off-machine results of manually taking the supernatant after centrifugation and the samples obtained by the automatic supernatant taking with the filter element in the example.
TABLE 4 off-line data results from manual supernatant extraction after centrifugation
| Sample numbering | Number of samples | Positive in primary examination | Positive rate | Number of failed samples | Failure rate |
| WH2201230001 | 90 | 18 | 20.0％ | 1 | 1.1％ |
| WH2201230002 | 90 | 22 | 24.4％ | 1 | 1.1％ |
| WH2201230003 | 90 | 22 | 24.4％ | 0 | 0.0％ |
| WH2201230005 | 90 | 22 | 24.4％ | 1 | 1.1％ |
| WH2201230006 | 90 | 25 | 27.8％ | 0 | 0.0％ |
| WH2201260004 | 90 | 24 | 26.7％ | 0 | 0.0％ |
| Total | 540 | 133 | 24.6％ | 3 | 0.6％ |
TABLE 5 off-line data results from automated supernatant extraction with filter element
| Sample plaitNumber (C) | Number of samples | Positive in primary examination | Positive rate | Number of failed samples | Failure rate |
| WH2201230001 | 90 | 30 | 33.3％ | 0 | 0.0％ |
| WH2201230002 | 90 | 30 | 33.3％ | 0 | 0.0％ |
| WH2201230003 | 90 | 30 | 33.3％ | 0 | 0.0％ |
| WH2201230005 | 90 | 30 | 33.3％ | 0 | 0.0％ |
| WH2201230006 | 90 | 30 | 33.3％ | 0 | 0.0％ |
| WH2201260004 | 90 | 30 | 33.3％ | 0 | 0.0％ |
| Total | 540 | 180 | 33.3％ | 0 | 0.0％ |
In tables 4 and 5, the number of failed samples is the number of samples in which sampling failed due to clogging of the tip.
As can be seen from the results in tables 4 and 5, the failure rate of the subsequent sequencing was significantly higher than that of the automatic supernatant taking with the filter element, without using the filter element, in the supernatant taking with centrifugation.
In the aspect of positive rate, the supernatant is taken manually after centrifugation, and the positive rate of the subsequent detection results fluctuates greatly and is poor in consistency. The positive rate of automatically taking supernatant by adding the filter element hardly fluctuates, and the consistency rate is high.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. Numerous simple deductions, modifications or substitutions may also be made by those skilled in the art in light of the present teachings.
Claims (10)
1. The filter element is characterized by comprising micropores for allowing a target substance to be separated in a sample liquid to pass through, wherein the pore diameter of the micropores of the filter element is 10-500 mu m.
2. The filter element according to claim 1, wherein the pore size of the pores of the filter element is 100 to 500 μm, preferably 300 to 500 μm, more preferably 350 to 400 μm, more preferably 375 μm.
3. The filter element of claim 1, wherein the material of the filter element comprises polyurethane, polypropylene, or polyethylene.
4. Filter element according to claim 1, wherein the density of the filter element is greater than or equal to 20kg/m 3 Preferably 25 to 35kg/m 3 More preferably 30kg/m 3 。
5. The filter cartridge of claim 1, wherein the filter cartridge is cylindrical;
preferably, the height of the filter element is more than or equal to 5mm, preferably 5-20 mm, and more preferably 5-10 mm;
preferably, the diameter of the filter element is more than or equal to 5mm, preferably 5-30 mm, and more preferably 10-20 mm.
6. A filter device, comprising the filter element of any one of claims 1 to 5 and a container for containing a sample liquid, wherein the filter element has micropores for passing a target substance to be separated in the sample liquid, and the filter element is configured to slide along an inner wall of the container to filter the sample liquid.
7. A filter device as claimed in claim 6, wherein at least part of the outer wall of the filter element is adapted to be snugly fitted to and slidable along the inner wall of a container containing the sample fluid.
8. Filter device according to claim 6, wherein the filter insert has a density of ≥ 20kg ≥m 3 Preferably 25 to 35kg/m 3 Preferably 30kg/m 3 ；
Preferably, the target comprises a cell;
preferably, the cells comprise tumor cells;
preferably, the tumor cells comprise colorectal cancerous tumor cells;
preferably, the sample fluid comprises a stool sample fluid;
preferably, the maximum diameter of the filter element is less than or equal to the inner diameter of a container for containing sample liquid, or slightly larger than the inner diameter of the container.
9. A method of filtering, comprising: the filter element of any one of claims 1 to 5 is placed in a container containing sample liquid, the filter element is pressed downwards, the sample liquid below the filter element in the container enters the filter element from the lower surface of the filter element, flows through the micropores of the filter element and then reaches the upper part of the filter element, and the liquid above the filter element is the filtered sample liquid.
10. The method of claim 9, further comprising drawing a desired sample fluid from the fluid above the filter element;
preferably, the control system controls the sampler to suck the required sample liquid from the liquid above the filter element;
preferably, the target comprises a cell;
preferably, the cells comprise tumor cells;
preferably, the tumor cells comprise colorectal cancerous tumor cells;
preferably, the sample fluid comprises a fecal sample fluid;
preferably, the maximum diameter of the filter element is less than or equal to the inner diameter of a container for containing sample liquid, or slightly larger than the inner diameter of the container.
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| US3512940A (en) * | 1968-12-30 | 1970-05-19 | Justin J Shapiro | Test tube filter device |
| US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
| US4643981A (en) * | 1983-11-09 | 1987-02-17 | Akzo N.V. | Pressure filtration system |
| US20010046452A1 (en) * | 2000-01-31 | 2001-11-29 | Roback John D. | Immunological assay system and method |
| US20090186341A1 (en) * | 2005-02-21 | 2009-07-23 | Hexal Aktiengesellschaft | Receptacle for the Separation of Tumor Cells |
| CN209221641U (en) * | 2015-09-02 | 2019-08-09 | 雅致有限公司 | Filter plant |
| CN210140589U (en) * | 2019-05-06 | 2020-03-13 | 广州市康立明生物科技有限责任公司 | Detection sample collection device |
| CN112827523A (en) * | 2021-01-08 | 2021-05-25 | 上海金鑫生物科技有限公司 | Ultrahigh molecular polyethylene filter containing activated carbon |
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2022
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3512940A (en) * | 1968-12-30 | 1970-05-19 | Justin J Shapiro | Test tube filter device |
| US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
| US4643981A (en) * | 1983-11-09 | 1987-02-17 | Akzo N.V. | Pressure filtration system |
| US20010046452A1 (en) * | 2000-01-31 | 2001-11-29 | Roback John D. | Immunological assay system and method |
| US20090186341A1 (en) * | 2005-02-21 | 2009-07-23 | Hexal Aktiengesellschaft | Receptacle for the Separation of Tumor Cells |
| CN209221641U (en) * | 2015-09-02 | 2019-08-09 | 雅致有限公司 | Filter plant |
| CN210140589U (en) * | 2019-05-06 | 2020-03-13 | 广州市康立明生物科技有限责任公司 | Detection sample collection device |
| CN112827523A (en) * | 2021-01-08 | 2021-05-25 | 上海金鑫生物科技有限公司 | Ultrahigh molecular polyethylene filter containing activated carbon |
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