CN217973213U - Cell culture device for imaging suspended organisms and microscopic imaging equipment - Google Patents
Cell culture device for imaging suspended organisms and microscopic imaging equipment Download PDFInfo
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- CN217973213U CN217973213U CN202221933314.3U CN202221933314U CN217973213U CN 217973213 U CN217973213 U CN 217973213U CN 202221933314 U CN202221933314 U CN 202221933314U CN 217973213 U CN217973213 U CN 217973213U
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Abstract
The utility model discloses a cell culture device and microscopic imaging equipment for being directed at suspended organism formation of image. The cell culture device comprises a culture dish body, wherein the culture dish body is provided with an accommodating cavity with an opening at the top, and the bottom of the accommodating cavity is provided with a slide for placing suspended organisms; the culture dish cover is covered on the top of the culture dish body to seal or open the top opening of the culture dish body; the top of the hanging bracket is hung on two sides of the inner wall of the culture dish body so that the bottom of the hanging bracket is close to the glass slide; a gauze is mounted on the bottom of the hanging bracket for compressing the suspended organisms between the gauze and the slide. The cell culture device compresses the suspended organisms between the gauze and the glass slide, so that the effect of fixing the suspended organisms can be achieved, the self-shaking of the suspended organisms is reduced, the stability of the suspended organisms in a culture solution is improved, and the microscopic imaging quality of the organisms is improved.
Description
Technical Field
The utility model relates to a microscopic imaging field of living cells especially relates to a cell culture device and microscopic imaging equipment for being directed at suspended organism formation of image.
Background
In the field of biological microscopic imaging, when experiments such as confocal microscopic imaging, fluorescence microscopic imaging and the like are carried out on suspended organisms including living cells and cell microspheres, the organisms need to be placed at a central through hole of a glass negative plate of a culture dish and then imaged by an imaging device.
In the conventional experiment of suspended cell imaging, one of the methods is to transfer cells onto a glass slide which is processed by gelatin, and after a series of processes, the glass slide is subjected to a mounting operation, and then the glass slide is placed under an imaging device to be observed, so that a required image is obtained. The seal is generally divided into a temporary seal and a permanent seal, and the temporary seal has the defects that the seal lacks gloss and the dyed background is difficult to remove after the seal; the permanent seal uses a seal agent which is a neutral gum capable of fading the background stain and increasing the gloss of the sheet, but has the disadvantage that the neutral gum is very sticky and is difficult to separate once sealed. In addition, when the organism is directly imaged on the confocal culture dish, in order to reduce the shaking of the organism itself and the imaging blur, the traditional method is to fix the cells by using a micro-needle or a method of extracting the culture solution, but the method has the disadvantages that the long-time observation cannot be performed and the organism is damaged in different degrees. Therefore, there is a need for a device that is both stable and simple to operate, and that can achieve clear imaging of suspended organisms.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the first technical problem that a cell culture device for being directed at suspended organism formation of image is provided to improve the stability of suspended organism in the culture solution, improve the microscopic image quality of organism.
Another technical problem to be solved by the present invention is to provide a microscopic imaging apparatus.
In order to realize the technical purpose, the utility model adopts the following technical scheme:
according to the utility model discloses an aspect of technical scheme provides a cell culture device for being directed at suspension organism formation of image, includes:
the culture dish comprises a culture dish body, wherein the culture dish body is provided with an accommodating cavity with an opening at the top, and a slide for placing suspended organisms is arranged at the bottom of the accommodating cavity;
the culture dish cover is covered on the top of the culture dish body to seal or open the top opening of the culture dish body;
the gauze support comprises a hanging support and a gauze, and the top of the hanging support is hung on two sides of the inner wall of the culture dish body so that the bottom of the hanging support is close to the glass slide; the gauze is mounted at the bottom of the hanging bracket for compressing the suspended organisms between the gauze and the slide.
Preferably, the culture dish body comprises:
the shell is provided with a containing cavity with an opening at the top, and a through hole with a preset shape is formed in the center of the bottom of the containing cavity;
the glass slide is pasted at the bottom of the shell so as to seal the through hole; wherein the shape of the slide matches the shape of the through hole.
Preferably, the outer wall of the top of the housing is provided with at least one hanging groove, the top of the hanging bracket is provided with at least one hook, and the hook is clamped with the hanging groove so that the hanging bracket is hung in the housing.
Preferably, the outer wall of the top of the shell is provided with a plurality of hanging grooves, and the hanging grooves are different in depth;
the hanging bracket can be hung in the hanging grooves with different depths so as to adjust the distance between the gauze and the slide.
Preferably, the outer wall of the top of the shell is provided with an annular limiting bulge, the inner wall of the culture dish cover is provided with an annular limiting clamping groove, and the annular limiting clamping groove is clamped with or separated from the annular limiting bulge to limit the relative position of the culture dish cover and the shell or relieve the position limitation.
Preferably, the glass slide, the through hole and the gauze are all circular, the diameter of the glass slide is larger than that of the through hole, and the diameter of the through hole is larger than that of the gauze.
Preferably, the housing is a plastic housing made of polystyrene and the slide is made of high transparent borosilicate glass.
Preferably, the diameter of the bottom surface of the shell is 32mm, the diameter of the through hole is 20mm, and the diameter of the slide is 26mm;
the thickness of culture dish body with the culture dish lid is 2mm, the thickness of slide is 0.17mm, the thickness of hanging support is 0.5mm.
Preferably, the screen is detachably mounted to the bottom of the hanging bracket.
According to the utility model discloses technical scheme's second aspect provides a microscopic imaging equipment, include:
the microscope is used for carrying out microscopic observation on suspended organisms;
the cell culture device is arranged at a preset position of the microscope;
and an image pickup unit provided at one side of the cell culture apparatus and used for image pickup.
Compared with the prior art, the utility model, beneficial effect as follows:
1. through compressing tightly the suspended organism between gauze and slide to can play the effect of fixed suspended organism, reduce the self of suspended organism and rock to a certain extent, improve the stability of suspended organism in the culture solution, improve the micro-imaging quality of organism.
2. Compared with the traditional mode of utilizing the micro-needle to draw the culture solution away to fix the suspended organism, the mode of adopting the gauze to fix the suspended organism in the embodiment can reduce the damage to the suspended organism, thereby prolonging the observation time and improving the observation effect.
Drawings
Fig. 1 is a schematic diagram of an overall structure of a cell culture apparatus for imaging suspended organisms according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an exploded structure of a cell culture apparatus for imaging suspended organisms according to an embodiment of the present invention;
fig. 3 is another schematic structural diagram of a housing according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a microscopic imaging apparatus according to an embodiment of the present invention.
In the drawings, each reference numeral denotes:
1. a culture dish body; 101. an accommodating chamber; 102. a through hole; 11. a housing; 12. glass slide; 111. hanging the groove; 112. an annular limiting bulge;
2. a culture dish cover;
3. a screen support; 31. a suspension bracket; 32. a screen; 301. hooking;
100. a microscope; 200. a cell culture device; 300. an image pickup unit.
Detailed Description
To make the objects, features and advantages of the present invention more obvious and understandable, the embodiments of the present invention are described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts all belong to the protection scope of the present invention.
As shown in fig. 1 and fig. 2, the cell culture apparatus for imaging suspended organisms provided by the embodiment of the present invention is mainly applied to biological microscopic imaging technology, and includes a culture dish body 1, a culture dish cover 2 and a gauze support 3.
The culture dish body 1 has a containing cavity 101 with an open top, and a slide 12 for placing suspended organisms is arranged at the bottom of the containing cavity 101. The culture dish cover 2 is covered on the top of the culture dish body 1 to seal or open the top opening of the culture dish body 1. As shown in fig. 2, the gauze frame 3 comprises a hanging frame 31 and a gauze 32, the top of the hanging frame 31 is hung on two sides of the inner wall of the culture dish body 1, so that the bottom of the hanging frame 31 is close to the slide 12; a screen 32 is mounted on the bottom of the hanging bracket 31 for compressing the suspended organisms between the screen 32 and the slide 12.
In this embodiment, in a specific use, a suspended organism is placed in the culture dish body 1 in advance for culture, and before imaging the suspended organism, first, the gauze 32 is aligned with the suspended organism to be imaged, the suspension holder 31 is moved toward the bottom of the accommodation chamber 101, and the suspended organism is pressed down to a position close to the bottom of the accommodation chamber 101; then, the plastic suspension bracket 31 is hung on the culture dish body 1, so that the gauze 32 can be stably positioned in the culture dish body 1; finally, the organism to be suspended is stabilized, and the organism can be observed through a microscope and can be imaged by an imaging part.
As shown in fig. 2, in the above embodiment, preferably, the culture dish body 1 includes the casing 11 and the slide 12. The housing 11 is a plastic housing made of polystyrene, the housing 11 has the accommodating cavity 101 with an open top, and a through hole 102 with a predetermined shape is opened at the center of the bottom of the accommodating cavity 101. The slide 12 is made of a high transparent borosilicate glass, and the slide 12 is attached to the bottom of the housing 11 so as to close the through hole 102. It will be appreciated that the shape of the slide matches the shape of the through-hole, and in this embodiment, the slide 12 and the through-hole 102 are both circular, and the diameter of the slide 12 is slightly larger, so that the through-hole 102 can be completely blocked. However, in other embodiments, the preset shape may also be an ellipse, a hexagon, or other shapes, and may be adaptively selected according to actual needs.
As shown in fig. 2, in the above embodiment, preferably, at least one hanging groove 111 is formed on an outer wall of the top of the housing 11, and at least one hook 301 is formed on the top of the hanging bracket 31, and the hook 301 is engaged with the hanging groove 111, so that the hanging bracket 31 is hung in the housing 11. Wherein, this hang recess 111 and couple 301's shape phase-match, in this embodiment, hang recess 111 and be squarely, couple 301 is square couple to can hang support 31 through the mutual block that hangs recess 111 and couple 301 hang in shell 11 on the one hand, on the other hand can avoid hanging support 31 and take place to rock after hanging the completion through the restriction of square shape, in order to improve holistic stability. It is understood that the shapes of the hanging groove 111 and the hook 301 may be adapted according to the need, and are not limited in particular.
In addition, in another embodiment, as shown in fig. 3, a plurality of hanging grooves 111 are formed on the outer wall of the top of the housing 11, and the hanging grooves 111 have different depths. Therefore, when the hanging bracket 31 needs to be installed, the hanging bracket 31 can be hung in hanging grooves 111 with different depths, so that the distance between the gauze 32 and the slide 12 can be adjusted to adapt to the sizes of different suspended microorganisms, and diversified observation requirements can be met. It will be appreciated that the plurality of hanging grooves 111 may be provided in pairs, that is, the hanging grooves 111 of the same depth are provided in pairs and symmetrically with respect to the central axis of the housing 11, thereby contributing to the improvement of the stability of the hanging.
As shown in fig. 2, in the above embodiment, an annular limiting protrusion 112 is disposed on an outer wall of the top of the housing 11, an annular limiting clamping groove (not shown) is disposed on an inner wall of the culture dish cover 2, and the annular limiting clamping groove is engaged with or separated from the annular limiting protrusion 112 to limit the relative position of the culture dish cover 2 and the housing 11 or to release the position limitation. Wherein, the internal diameter of this culture dish lid 2 slightly is greater than the external diameter of shell 11 to after culture dish lid 2 lock closure is at the top of shell 11, the spacing draw-in groove of annular can with the spacing protruding 112 looks block of annular, and make the inner wall of culture dish lid 2 laminate mutually with the top of culture dish body 1 just, in order to guarantee sealed effect. It can be understood that, in another embodiment, an annular limiting clamping groove may be formed in the outer shell 11, and an annular limiting protrusion is arranged on the inner wall of the culture dish cover 2, so as to achieve the same effect.
In the above embodiment, the slide 12, the through hole 102, and the gauze 32 are all circular, and the diameter of the slide 12 is larger than the diameter of the through hole 102, and the diameter of the through hole 102 is larger than the diameter of the gauze 32. Specifically, in this embodiment, the diameter of the bottom surface of the housing 11 is 32mm, the diameter of the through hole 102 is 20mm, and the diameter of the slide 12 is 26mm; the thickness of culture dish body 1 and culture dish lid 2 is 2mm, and the thickness of slide 12 is 0.17mm, and the thickness of hanging support 31 is 0.5mm. In this embodiment, the sizes of the components are configured to be the optimal sizes, which can form the optimal observation effect, and in other embodiments, the sizes can be adaptively adjusted according to different observation requirements.
In the above embodiment, it is preferable that the gauze 32 is detachably mounted to the bottom of the hanging bracket 31. Specifically, in this embodiment, the hanging bracket 31 is made of a plurality of rigid plastic sheets, which are commonly enclosed in a cylindrical shape, so that the gauze 32 can be enclosed at the bottom of the rigid plastic sheets and can be detached for cleaning or replacing the gauze 32. Further, the hooks 301 are provided on the top of two symmetrical rigid plastic sheets among the plurality of rigid plastic sheets, so as to be hung on the housing 11.
As shown in fig. 4, in addition to the cell culture apparatus provided in the above embodiment, the embodiment of the present invention further provides a microscopic imaging apparatus, which includes a microscope 100, the above cell culture apparatus 200, and an image capturing unit 300. Specifically, the microscope 100 is used for microscopic observation of suspended organisms; the cell culture apparatus 200 is disposed at a predetermined position of the microscope 100 for observation by the microscope; the image pickup unit 300 is provided at one side of the cell culture apparatus 200 for image pickup.
To sum up, the embodiment of the utility model provides a be used for to suspension organism imaging's cell culture device and microscopic imaging equipment compresses tightly between gauze 32 and slide 12 through with the suspension organism to can play fixed suspension organism's effect, reduce suspension organism's self to a certain extent and rock, improve the stability of suspension organism in the culture solution, improve the microscopic imaging quality of organism. In addition, compared with the traditional mode of using a microneedle to draw the culture solution away to fix the suspended organism, the mode of using the gauze 32 to fix the suspended organism in the embodiment can reduce the damage to the suspended organism, thereby prolonging the observation time and improving the observation effect.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Moreover, various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without being mutually inconsistent.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A cell culture apparatus for imaging suspended organisms, comprising:
the culture dish comprises a culture dish body, wherein the culture dish body is provided with an accommodating cavity with an opening at the top, and a slide for placing suspended organisms is arranged at the bottom of the accommodating cavity;
the culture dish cover is covered on the top of the culture dish body to seal or open the top opening of the culture dish body;
the top of the hanging bracket is hung on two sides of the inner wall of the culture dish body, so that the bottom of the hanging bracket is close to the glass slide; the gauze is mounted at the bottom of the hanging bracket for compressing the suspended organisms between the gauze and the slide.
2. The cell culture apparatus of claim 1, wherein the culture dish body comprises:
the shell is provided with an accommodating cavity with an opening at the top, and a through hole in a preset shape is formed in the center of the bottom of the accommodating cavity;
the glass slide is pasted at the bottom of the shell so as to seal the through hole; wherein the shape of the slide matches the shape of the through hole.
3. The cell culture apparatus of claim 2, wherein the outer wall of the top of the housing defines at least one hanging groove, and the top of the hanging bracket defines at least one hook that engages the hanging groove to suspend the hanging bracket within the housing.
4. The cell culture apparatus of claim 2, wherein the outer wall of the top of the housing defines a plurality of hanging grooves, and wherein the hanging grooves have different depths;
the hanging bracket can be hung in the hanging grooves with different depths so as to adjust the distance between the gauze and the slide.
5. The cell culture device according to claim 2, wherein an annular limiting protrusion is provided on an outer wall of the top of the housing, an annular limiting clamping groove is provided on an inner wall of the culture dish cover, and the annular limiting clamping groove is engaged with or separated from the annular limiting protrusion to limit a relative position of the culture dish cover and the housing or to release the position limitation.
6. The cell culture apparatus of claim 2, wherein the slide, the through hole, and the screen are circular, and wherein the diameter of the slide is greater than the diameter of the through hole, and wherein the diameter of the through hole is greater than the diameter of the screen.
7. The cell culture apparatus of claim 2, wherein the housing is a plastic housing made of polystyrene and the slide is made of high-transparency borosilicate glass.
8. The cell culture apparatus of claim 2, wherein the bottom surface of the housing has a diameter of 32mm, the through-hole has a diameter of 20mm, and the slide has a diameter of 26mm;
the thickness of culture dish body with the culture dish lid is 2mm, the thickness of slide is 0.17mm, the thickness of hanging support is 0.5mm.
9. The cell culture apparatus of claim 1, wherein the screen is removably mounted to the bottom of the hanging bracket.
10. A microscopic imaging apparatus, comprising:
the microscope is used for carrying out microscopic observation on suspended organisms;
the cell culture assembly of any one of claims 1-9, disposed in a predetermined position of the microscope;
and the image pickup part is arranged on one side of the cell culture device and is used for image pickup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221933314.3U CN217973213U (en) | 2022-07-22 | 2022-07-22 | Cell culture device for imaging suspended organisms and microscopic imaging equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221933314.3U CN217973213U (en) | 2022-07-22 | 2022-07-22 | Cell culture device for imaging suspended organisms and microscopic imaging equipment |
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| CN217973213U true CN217973213U (en) | 2022-12-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116694441A (en) * | 2023-06-27 | 2023-09-05 | 广东省农业科学院植物保护研究所 | Insect cell culture dish convenient to take slide |
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2022
- 2022-07-22 CN CN202221933314.3U patent/CN217973213U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116694441A (en) * | 2023-06-27 | 2023-09-05 | 广东省农业科学院植物保护研究所 | Insect cell culture dish convenient to take slide |
| CN116694441B (en) * | 2023-06-27 | 2024-03-08 | 广东省农业科学院植物保护研究所 | Insect cell culture dish convenient to take slide |
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