CN217973132U

CN217973132U - Closed nucleic acid extraction kit

Info

Publication number: CN217973132U
Application number: CN202220951438.8U
Authority: CN
Inventors: 章路平; 万莉平; 朱春森; 王弼陡; 马后师
Original assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Current assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Priority date: 2022-04-22
Filing date: 2022-04-22
Publication date: 2022-12-06
Anticipated expiration: 2032-04-22

Abstract

The utility model provides a closed nucleic acid extraction kit, include: a base; a reagent cartridge body; the reagent box body is provided with a plurality of cavities, a plurality of first flow channels and a plurality of second flow channels; the outlet of the first flow channel is provided with a closed structure; the top and the bottom of the cavity are respectively communicated with a first flow passage and a second flow passage; the cavity is used for containing a reagent or a sample; a sliding member slidably coupled to the reagent cartridge body; the sliding part is provided with a cavity and a concave cavity; one end of the concave cavity is used for being connected with the injector, and the other end of the concave cavity is communicated with one side of the cavity; the bottom wall of the cavity is provided with a first through hole; the reaction tube is connected to the reagent box body and is respectively communicated with a first flow passage and a second flow passage. Overall structure is small and exquisite, and the second runner that communicates each other, appearance chamber, cavity form a cavity jointly, and liquid transfer in-process nucleic acid is sealed inside the kit body, avoids taking place the aerosol and leaks and pollute external environment.

Description

Closed nucleic acid extraction kit

Technical Field

The utility model relates to a nucleic acid extraction kit technical field especially relates to a closed nucleic acid extraction kit.

Background

In molecular biology experiments, nucleic acid extraction is one of the most basic and important links, and the yield, purity and integrity of nucleic acid extraction is directly related to the success or failure of downstream nucleic acid detection, biological research or other new product development.

The extraction kit based on the paramagnetic particle method on the existing market extracts and is equipped with a plurality of cavities for hold required reagents in extraction processes such as lysate, washing liquid, eluant, when shifting the reagent, the cavity is opened, easily leads to the aerosol to pollute.

SUMMERY OF THE UTILITY MODEL

In order to achieve the above purpose, the utility model is realized by the following technical scheme.

The utility model provides a closed nucleic acid extraction kit, include:

a base to form a support structure;

a reagent cartridge body mounted to the base; the reagent box body is provided with a plurality of cavities, a plurality of first flow channels and a plurality of second flow channels; the outlet of the first flow channel is provided with a closed structure which is used for balancing the air pressure inside and outside the first flow channel and is not permeable to nucleic acid; the top and the bottom of the cavity are respectively communicated with the first flow passage and the second flow passage; the cavity is used for containing a reagent or a sample;

a slider slidably connected to the reagent cartridge body; the sliding part is provided with a cavity and a concave cavity; one end of the concave cavity is used for being connected with an injector, and the other end of the concave cavity is communicated with one side of the cavity; the bottom wall of the cavity is provided with a first through hole;

the reaction tube is connected to the reagent box body and is respectively communicated with the first flow channel and the second flow channel;

the sliding piece slides relative to the reagent box body, so that the first through hole is communicated with the corresponding second flow channel, and the reagent or the sample is sucked and spitted under the cooperation of the injector.

Preferably, the accommodating cavity is of a through groove structure; the first flow channel is positioned on the upper surface of the reagent box body and is open above the reagent box body; the second flow channel is positioned on the lower surface of the reagent box body, and the lower part of the second flow channel is open;

the upper openings of the accommodating cavity and the first flow channel are abutted against the lower surface of a first sealing element, and the first sealing element is provided with a plurality of third through holes to respectively form outlets of a plurality of first flow channels; the cavity and the opening below the second flow channel are abutted against the upper surface of the sealing bottom plate.

Preferably, one side of the sliding piece facing the first flow channel is provided with an extension part; the extension part is provided with at least one second through hole; the extension abuts the first seal upper surface;

when the first through hole communicates with the corresponding second flow passage, the third through hole corresponding to the second flow passage communicates with the second through hole.

Preferably, the reagent cartridge further comprises a pressing cover fixed to the reagent cartridge body and pressed against the sliding member and the upper surface of the first sealing member.

Preferably, the upper surface of the pressing cover is provided with a plurality of first positioning parts, and the sliding part is provided with a second positioning part; the second positioning part is matched with the first positioning part so as to verify the communication condition of the first through hole and the corresponding second flow channel.

Preferably, the sliding part is provided with a bump for bearing a pushing force to drive the sliding part to slide.

Preferably, the enclosing structure is a nucleic acid filter cotton or a sealing cover.

Preferably, a second sealing element is arranged between the second flow passage and the first through hole.

Preferably, one of the cavities is a sample position, and a sealing cover is arranged at an open position above the cavity.

Preferably, the sample site is located adjacent to one end of the reagent cartridge body.

Compared with the prior art, the beneficial effects of the utility model reside in that:

the utility model provides a closed nucleic acid draws kit, kit body overall structure is small and exquisite, and the leakproofness is good, and the second runner that communicates each other, appearance chamber, cavity form a cavity jointly to supply liquid to shift, liquid shift in-process nucleic acid is sealed inside the kit body, avoids taking place the aerosol and leaks and pollute external environment.

The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the specification, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings. The detailed description of the present invention is given by the following examples and the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention and do not constitute a limitation on the invention. In the drawings:

fig. 1 is a schematic diagram of an explosion structure of a kit body of the present invention;

fig. 2 is a schematic perspective view of the reagent cartridge of the present invention;

FIG. 3 is a bottom view of the reagent cartridge body of the present invention;

fig. 4 is a schematic perspective view of the sliding member of the present invention;

fig. 5 is a schematic perspective view of a second sealing member according to the present invention;

fig. 6 is a structural sectional view of the cartridge body of the present invention;

fig. 7 is a schematic perspective view of the reagent kit body of the present invention.

In the figure: 10. a kit body;

11. a base;

12. a reagent cartridge body; 121. a cavity; 1211. a first cavity; 1212. a second cavity; 1213. a third cavity; 1214. a fourth cavity; 1215. a fifth cavity; 1216. a sixth cavity; 1217. a seventh cavity; 1218. an eighth cavity; 1219. a ninth cavity; 122. a first flow passage; 123. a second flow passage; 124. a groove;

13. a slider; 131. a cavity; 1311. a first through hole; 132. a concave cavity; 133. an extension portion; 1331. a second through hole; 134. a second positioning portion; 135. a bump;

14. a reaction tube;

15. a first seal member; 151. a third through hole;

16. a gland; 161. a first positioning portion; 162. a yielding through groove; 163. a second abdication gap;

17. a second seal member; 171. a fourth via hole; 172. a boss portion;

18. a sealing cover;

19. and sealing the bottom plate.

Detailed Description

The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the annexed drawings, discloses a more detailed description of the present invention, which will enable those skilled in the art to make and use the present invention. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and generally are not intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.

Example 1

The utility model provides a closed nucleic acid extraction kit, as shown in fig. 1 to 7, including kit body 10, kit body 10 includes:

a base 11 to form a support structure;

a reagent cartridge 12 attached to the base 11; the reagent cartridge body 12 is provided with a plurality of cavities 121, a plurality of first flow channels 122 and a plurality of second flow channels 123; the outlet of the first flow channel 122 is provided with a closed structure for balancing the air pressure inside and outside the first flow channel 122 and preventing nucleic acid from permeating; the top and the bottom of the cavity 121 are respectively communicated with the first flow passage 122 and the second flow passage 123; the cavity 121 is used for accommodating a reagent or a sample;

a slider 13 slidably connected to the reagent cartridge body 12; the sliding part 13 is provided with a cavity 131 and a cavity 132; one end of the cavity 132 is used for connecting with a syringe, and the other end is communicated with one side of the cavity 131; the bottom wall of the cavity 131 is provided with a first through hole 1311;

the reaction tube 14 is connected to the reagent cartridge 12, and is respectively communicated with the first flow channel 122 and the second flow channel 123;

the sliding member 13 slides relative to the reagent cartridge 12, so that the first through hole 1311 communicates with the corresponding second flow channel 123, and the reagent or the sample is aspirated and spitted by the syringe.

In this embodiment, the kit body 10 includes a base 11, a reagent box 12, a sliding member 13, and a reaction tube 14, and with the cooperation of the syringe, the operations of pipetting, sucking, spitting, and mixing of the reagent are realized, and the operations of cracking, mixing, washing, and eluting are realized, thereby completing the nucleic acid extraction. The whole structure of the kit body 10 is small and exquisite, the sealing performance is good, the second flow channel 123, the containing cavity 121 and the cavity 131 which are communicated with each other form a cavity together for liquid transfer, nucleic acid is sealed inside the kit body 10 in the liquid transfer process, and the aerosol leakage is prevented from polluting the external environment.

In an embodiment, as shown in fig. 1, fig. 2, fig. 3, and fig. 6, the number of the cavities 121 is nine, and specifically includes a first cavity 1211, a second cavity 1212, a third cavity 1213, a fourth cavity 1214, a fifth cavity 1215, a sixth cavity 1216, a seventh cavity 1217, an eighth cavity 1218, and a ninth cavity 1219; the first cavity 1211 is for receiving a sample, i.e., a sample site; the second cavity 1212 is used for holding lysis solution; the third cavity 1213 is for containing a binding liquid; the fourth cavity 1214 is used for containing cleaning liquid a; a fifth chamber 1215 for holding magnetic beads; the sixth cavity 1216 is for containing a cleaning solution B; seventh volume 1217 contains the eluent; the eighth cavity 1218 is for holding a reactant; the ninth chamber 1219 is for containing water. The volume of the ninth chamber 1219 is maximized when the amount of water used in the nucleic acid extraction process is large. Further, ninth cavity 1219 is located on one side of slide 13, and first cavity 1211, second cavity 1212, third cavity 1213, fourth cavity 1214, fifth cavity 1215, sixth cavity 1216, seventh cavity 1217 and eighth cavity 1218 are located on the other side of slide 13.

Specifically, the pipetting principle is illustrated by cell lysis as an example. The cavity 132 is connected with a syringe, the sliding member 13 moves to a position where the first cavity 1211 is communicated with the cavity 131, the syringe absorbs liquid so that the sample in the first cavity 1211 enters the cavity 131, the sliding member 13 moves to a position where the second cavity 1212 is communicated with the cavity 131, the syringe spits the liquid, and the sample is spitted into the second cavity 1212 to be mixed with the lysis solution, so that lysis is achieved. Through the adjustment of the position of the sliding part 13, the transfer of different liquids is realized, and then the extraction of nucleic acid is realized. The operation of transferring the liquid is similar to the operation of cell lysis, and will not be described herein. When the fifth cavity 1215 is cleaned, since the fifth cavity 1215 contains magnetic beads, the cleaning is performed after the magnetic beads are attracted by an external magnetic attraction mechanism.

In one embodiment, as shown in fig. 2 and 6, the reagent cartridge body 12 is U-shaped, and the sliding member 13 is disposed in the U-shaped groove of the reagent cartridge body 12. The reagent box body 12 has a simple structure and is compactly assembled with the sliding part 13, which is beneficial to the miniaturization design of the reagent box body 10. The port of the second flow channel 123 is provided at the bottom of the reagent cartridge body 12 to facilitate alignment with the first through hole 1311 at the bottom of the cavity 131 of the slider 13.

In an embodiment, as shown in fig. 2 and 3, the cavity 121 is a through-slot structure; the first flow channel 122 is located on the upper surface of the reagent cartridge body 12 and is open above the reagent cartridge body; the second flow channel 123 is located on the lower surface of the reagent cartridge body 12 and is open below the lower surface;

as shown in fig. 1 and fig. 6, the accommodating cavity 121 and the first flow channel 122 are open above and abut against the lower surface of the first sealing element 15, and the first sealing element 15 is provided with a plurality of third through holes 151 to form a plurality of outlets of the first flow channel 122, respectively; the lower part of the cavity 121 and the second flow passage 123 is open to abut against the upper surface of the sealing bottom plate 19. Specifically, the structure design of the cavity 121, the first flow channel 122 and the second flow channel 123 facilitates processing and reduces the processing difficulty. A relatively closed cavity is formed by the first sealing element 15, the sealing bottom plate 19 and the accommodating cavity 121 together and is used for accommodating a reagent or a sample; a relatively closed air pressure balance channel is formed by the first sealing element 15 and the first flow passage 122; the sealing bottom plate 19 and the second flow channel 123 together form a relatively closed liquid transfer channel structure.

Further, as shown in fig. 1, 4, and 6, an extension 133 is disposed on one side of the sliding member 13 facing the first flow passage 122; the extending portion 133 is provided with at least one second through hole 1331; the extension 133 abuts against the upper surface of the first seal 15;

the third through holes 151 are respectively in one-to-one correspondence with and communicated with the outlets of the first flow channels 122; when the first through holes 1311 communicate with the respective second flow passages 123, the third through holes 151 corresponding to the second flow passages 123 communicate with the second through holes 1331. Specifically, the extension portion 133 and the first sealing element 15 are matched with the first flow channel 122 to form a structure of an exchange port between the first flow channel 122 and the external air, so that in the operation process of the syringe, a liquid transfer cavity formed by the first flow channel 122, the accommodating cavity 121, the second flow channel 123 and the cavity 131 can maintain balance of internal and external air pressures, so as to facilitate suction and pushing of the syringe. Further, the arrangement of the extension 133 and the first sealing member 15 can reduce the possibility of nucleic acids escaping from the first channel 122.

In a specific embodiment, as shown in fig. 1 and 6, the number of the cavities 121 is nine, the ninth cavity 1219 is located on one side of the sliding member 13, and the rest of the cavities are located on the other side of the sliding member 13, so that the two sides of the sliding member 13 are both provided with the extending portions 133, and the two first sealing members 15 are respectively matched with the cavities 121 located on the two sides of the sliding member 13, so as to ensure that the air pressure in the liquid drawing or pushing process is balanced after each cavity 121 is communicated with the cavity 131.

Further, the reagent cartridge body 10 further includes a pressing cover 16 fixed to the reagent cartridge body 12 and pressed against the upper surfaces of the slider 13 and the first sealing member 15. Specifically, the upper surface of the slider 13 is pressed by the pressing cover 16, so that the lower surface of the slider 13 is tightly pressed against the reagent cartridge body 12 to ensure the tightness of the place where the cavity 131 communicates with the second flow channel 123. Further, the upper surface of the first sealing member 15 is pressed by the pressing cover 16 to achieve the fitting of the first sealing member 15.

Further, the upper surface of the gland 16 is provided with a plurality of first positioning portions 161, and the sliding member 13 is provided with a second positioning portion 134; the second positioning portion 134 is matched with the first positioning portion 161 to verify the communication between the first through hole 1311 and the corresponding second flow channel 123. Specifically, the sliding of the sliding member 13 relative to the reagent cartridge 12 may be performed by an external mechanical force or a manual force, and by the mechanical force, the sliding distance of the sliding member 13 may be precisely controlled by the design of the control unit, and when the control unit is out of order, by observing the positioning conditions of the second positioning portion 134 and the first positioning portion 161, and by timing the abnormal conditions. When manual force is used, the accuracy of displacement of the sliding member 13 is improved by observing the positioning conditions of the second positioning portion 134 and the first positioning portion 161.

Further, the sliding member 13 is provided with a protrusion 135 for bearing a pushing force to drive the sliding member 13 to slide. Further, the pressing cover 16 is provided with a yielding through groove 162 for yielding the movement of the projection 135 of the slider 13.

In one embodiment, the enclosing structure is a nucleic acid filter cotton or a sealing cover. Specifically, the nucleic acid filter cotton has air permeability and is capable of preventing nucleic acids from passing through. The sealing cover can prevent nucleic acid from passing through and gas stored between the sealing cover and the reagent cartridge 12, so as to balance the gas pressure inside and outside the first flow channel 122.

In an embodiment, as shown in fig. 1 and 6, a second sealing member 17 is disposed between the second flow channel 123 and the first through hole 1311 to improve the tightness at the communication position between the second flow channel 123 and the first through hole 1311. The second sealing member 17 is provided with a plurality of fourth through holes 171 to allow the second flow channel 123 to communicate with the first through hole 1311.

Further, as shown in fig. 2, 5 and 6, the reagent cartridge body 12 is provided with a plurality of grooves 124, and a port of a second flow channel 123 is disposed in one groove 124; the bottom of the second sealing member 17 is provided with a plurality of protrusions 172, and a fourth through hole 171 is arranged in one of the protrusions 172; the protrusion 172 is engaged in the groove 124 to clamp the second sealing member 17 to the reagent cartridge 12, and to improve the sealing property of the connection portion of the fourth through hole 171 and the second flow channel 123.

In one embodiment, as shown in fig. 1 and 7, one of the cavities 121 is a sample position, a sealing cover 18 is disposed at an open position above the cavity, and the sealing cover 18 is opened to sample the sample, so as to facilitate sample loading.

In one embodiment, as shown in FIGS. 1 and 7, the sample site is located near one end of the reagent cartridge body 12 so as not to interfere with the assembly of the remaining components. In one embodiment, the cartridge body 10 includes a gland 16, the gland 16 is secured to the reagent cartridge body 12, and the sample site is positioned adjacent to an end of the reagent cartridge body 12 to allow for assembly of the gland 16 to the reagent cartridge body 12. Further, the gland 16 is provided with an out-of-way notch 163 to allow for the provision of the sealing cap 18.

In one embodiment, as shown in fig. 7, the cartridge body 10 has a flat structure, a small size, and a small occupied space.

In one embodiment, the kit body 10 is a disposable consumable structure, each reagent is pre-loaded in a corresponding cavity, when nucleic acid extraction is performed, a sample is added into the kit body 10 and then assembled with an injector, so that nucleic acid extraction can be performed, and the operation is simple and convenient. Further, the syringe may be an external accessory or may be assembled with the cavity 132 to form a disposable consumable structure.

In one embodiment, as shown in fig. 1 and 7, the reaction tube 14 is installed at one side of the reagent cartridge body 12 so that the reagent cartridge body 10 is engaged with the nucleic acid amplification device at one side thereof to perform a subsequent nucleic acid amplification operation.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way; the utility model can be smoothly implemented by the ordinary technicians in the industry according to the drawings and the above description; however, those skilled in the art should understand that the equivalent embodiments of the present invention are equivalent embodiments of the present invention, and that the changes, modifications and evolutions made by the above-disclosed technical contents are not departed from the technical scope of the present invention; meanwhile, any changes, modifications, evolutions, etc. of equivalent changes made to the above embodiments according to the essential technology of the present invention still belong to the protection scope of the technical solution of the present invention.

Claims

1. A closed nucleic acid extraction kit, characterized by comprising:

a base (11) to form a support structure;

a reagent cartridge (12) attached to the base (11); the reagent box body (12) is provided with a plurality of cavities (121), a plurality of first flow channels (122) and a plurality of second flow channels (123); the outlet of the first flow channel (122) is provided with a closed structure which is used for balancing the air pressure inside and outside the first flow channel (122) and is not permeable to nucleic acid; the top and the bottom of the cavity (121) are respectively communicated with the first flow passage (122) and the second flow passage (123); the cavity (121) is used for containing a reagent or a sample;

a slide (13) slidably connected to the reagent cartridge (12); the sliding piece (13) is provided with a cavity (131) and a cavity (132); one end of the cavity (132) is used for being connected with an injector, and the other end of the cavity is communicated with one side of the cavity (131); the bottom wall of the cavity (131) is provided with a first through hole (1311);

a reaction tube (14) connected to the reagent cartridge (12) and respectively communicating with the first flow channel (122) and the second flow channel (123);

the sliding piece (13) slides relative to the reagent box body (12) so that the first through hole (1311) is communicated with the corresponding second flow channel (123), and the reagent or the sample is sucked and spitted under the cooperation of the injector.

2. The closed nucleic acid extraction kit as claimed in claim 1, wherein the cavity (121) has a through-slot structure; the first flow channel (122) is positioned on the upper surface of the reagent box body (12) and is open above the reagent box body; the second flow channel (123) is positioned on the lower surface of the reagent box body (12) and is open below the reagent box body;

the accommodating cavity (121) and an opening above the first flow channel (122) are abutted to the lower surface of a first sealing element (15), and the first sealing element (15) is provided with a plurality of third through holes (151) to form outlets of the first flow channels (122) respectively; the lower parts of the accommodating cavity (121) and the second flow passage (123) are open and are abutted against the upper surface of the sealing bottom plate (19).

3. The closed nucleic acid extraction kit as claimed in claim 2, wherein the side of the sliding member (13) facing the first flow channel (122) is provided with an extension (133); the extension part (133) is provided with at least one second through hole (1331); the extension (133) abuts against the first seal (15) upper surface;

when the first through-holes (1311) communicate with the respective second flow passages (123), the third through-holes (151) corresponding to the second flow passages (123) communicate with the second through-holes (1331).

4. The closed nucleic acid extraction kit according to claim 2, further comprising a cover (16) fixed to the reagent cartridge body (12) and pressed against the upper surfaces of the slide member (13) and the first sealing member (15).

5. The closed nucleic acid extraction kit according to claim 4, wherein the upper surface of the cover (16) is provided with a plurality of first positioning portions (161), and the sliding member (13) is provided with a second positioning portion (134); the second positioning part (134) is matched with the first positioning part (161) so as to verify the communication condition of the first through hole (1311) and the corresponding second flow passage (123).

6. The closed nucleic acid extraction kit as claimed in any one of claims 1 to 5, wherein the sliding member (13) has a protrusion (135) for receiving a pushing force to slide the sliding member (13).

7. The closed nucleic acid extraction kit according to any one of claims 1 to 5, wherein the closed structure is a nucleic acid filter cotton or a sealing cover.

8. The closed nucleic acid extraction kit according to any one of claims 1 to 5, wherein a second sealing member (17) is provided between the second flow channel (123) and the first through hole (1311).

9. The closed nucleic acid extraction kit as claimed in any one of claims 1 to 5, wherein one of the chambers (121) is a sample site, and a sealing cover (18) is provided at an open upper portion thereof.

10. The closed nucleic acid extraction kit according to claim 9, wherein the sample site is disposed near one end of the reagent cartridge body (12).