CN218620840U - Amplification card box and nucleic acid detection equipment - Google Patents

Abstract

The utility model discloses an amplification card box and nucleic acid detection equipment, the amplification card box includes card box base, a plurality of amplification pipes and a plurality of cavity combination, the cavity combination includes first cavity, second cavity, first mixing cavity, second mixing cavity, third cavity and fourth cavity, the preserving fluid is deposited in the first cavity, the cleaning fluid is deposited in the second cavity, the lysate is deposited in the second mixing cavity, the third cavity is used for communicating the air supply, the eluent is deposited in the fourth cavity; the cartridge base is provided with a plurality of fluid path assemblies. Each cavity of the cavity combination is correspondingly switched on and off with each liquid path of the liquid path combination, so that sample liquid mixing, washing, residual liquid cleaning, elution and liquid discharging are realized, the extraction and detection efficiency is improved, and the waste liquid is stored in the first cavity, so that the structure utilization rate is improved. The amplification card box is designed to be provided with a plurality of chamber combinations and a plurality of liquid path combinations, so that a multi-channel parallel card box structure is obtained, and sample liquid can be synchronously processed. The utility model can be widely applied to the technical field of biomedical instruments.

Description

Amplification card box and nucleic acid detection equipment

Technical Field

The utility model relates to a biological medicine instrument technical field, in particular to increase card box and nucleic acid check out test set.

Background

In the biomolecule detection, the transfer and specific operation between liquids are involved, and in the transfer process, most of the methods adopt open suction, transfer or pouring among reagent tubes, which easily causes volatile toxic reagents to volatilize and diffuse to the outside of the container, pollutes the external environment and endangers the personal safety of operators.

In recent years, rapid and high-throughput detection becomes a new requirement for pathogen detection, the detection must be safe and rapid, and both traditional manual detection and existing automatic detection equipment inevitably cause external pollution to a use scene, so that specialized planning operation is required.

In addition, although the current commercialized nucleic acid detection equipment also has full-automatic detection equipment integrating extraction, amplification and detection, most of the equipment adopts a single-channel card box or completes high flux in a mode of overlapping the single-channel card boxes, so that the requirement of the nucleic acid detection equipment on high flux is difficult to meet, and the detection efficiency is not high.

SUMMERY OF THE UTILITY MODEL

In order to solve at least one of the above technical problems, the present invention provides an amplification cassette and a nucleic acid detecting apparatus, which adopt the following technical solutions.

The utility model provides an amplification card box includes card box base, a plurality of amplification pipes and a plurality of cavity combination, the cavity combination with the card box base is connected, the amplification pipe with the card box base is connected, the cavity combination includes first cavity, second cavity, first mixing chamber, second mixing chamber, third cavity and fourth cavity, save liquid can be deposited in the first cavity, the first cavity with the card box base is connected; the second chamber is connected with the cartridge base, washing liquid can be stored in the second chamber, and the second chamber is provided with a second plunger; the first mixing chamber is connected with the cartridge base, and is provided with a first mixing plunger; the second mixing chamber is connected with the card box base, lysate can be stored in the second mixing chamber, and a second mixing plunger is arranged in the second mixing chamber; the third chamber is connected with the card box base and is used for being communicated with a gas source; the fourth chamber is connected with the cassette base, eluent can be stored in the fourth chamber, and a fourth plunger is arranged in the fourth chamber; the card box base is provided with a plurality of liquid path combinations, each liquid path combination comprises a first liquid path, a second liquid path, a third liquid path and a fourth liquid path, the first liquid paths are respectively used for connecting the first cavity, the second cavity and the first mixing cavity, the second liquid paths are respectively used for connecting the first mixing cavity and the second mixing cavity, the third liquid paths are respectively used for connecting the second mixing cavity, the third cavity and the fourth cavity, the fourth liquid paths are connected with the amplification pipes, and the liquid paths in the liquid path combinations are respectively and synchronously controlled to be switched on and off through movably connected switch valves.

In some embodiments of the present invention, a magnetic element receiving area is disposed between the first mixing chamber and the second mixing chamber.

In some embodiments of the invention, the amplification cartridge comprises a magnetic element, the magnetic element being in the magnetic element receiving area can be close to or away from the magnetic attraction chamber of the cartridge base in a direction perpendicular to the surface of the cartridge base.

In some embodiments of the present invention, the chamber combination includes a fifth chamber, the fifth chamber with the cartridge base is connected, the fifth chamber is used for depositing mineral oil or gas, the fifth chamber is provided with a fifth plunger, the fifth chamber communicates the third fluid path, just the fifth chamber with but the connection of third fluid path break-make.

In some embodiments of the present invention, each of the switch valves is slidably connected to the cartridge base.

The utility model discloses a certain embodiment, two opposite sides of card box base are provided with respectively the liquid way combination, first liquid way the second liquid way the third liquid way is in respectively the relative bilateral symmetry of card box base sets up, be provided with a plurality of flow paths in the ooff valve, the flow path is in the side of ooff valve forms first intercommunication mouth and second intercommunication mouth, each first intercommunication mouth is in one or two are arranged into to the side of ooff valve, respectively the second intercommunication mouth is located respectively two opposite sides of ooff valve.

In some embodiments of the present invention, the amplification card box comprises a plurality of covers, and the covers are connected to the first chamber in a split or integrated manner.

In some embodiments of the present invention, a filter element is disposed in the cover.

In certain embodiments of the present invention, the third chamber is provided with a filter element.

In certain embodiments of the present invention, the second mixing plunger is configured with a travel control element that controls the distance of movement of the second mixing plunger.

In some embodiments of the present invention, the amplification cartridge comprises four chamber assemblies, four liquid path assemblies and four amplification tubes.

The utility model provides a nucleic acid detecting equipment includes the amplification card box.

The utility model provides a nucleic acid detection equipment includes the amplification card box, the amplification card box sets up to 12, the amplification pipe in the amplification card box distributes the overall arrangement according to 4 x 12's array, and arrange each the amplification card box into one row and set up in nucleic acid detection equipment; or the amplification cartridges are arranged in 24, the amplification tubes in the amplification cartridges are distributed according to an array of 4 x 12 x 2, and the amplification cartridges are symmetrically arranged in two rows in the nucleic acid detection device.

The utility model discloses but wide application in biomedical instrument technical field, the utility model discloses an embodiment has following beneficial effect at least:

each chamber in the chamber combination of the amplification card box is correspondingly switched on and off with each liquid path in the liquid path combination, so that sample liquid mixing, washing, residual liquid cleaning, elution and liquid discharging are continuously realized in a closed environment, the extraction and detection efficiency is improved, the generated waste liquid can be stored in the first chamber, and the structure utilization rate is improved.

The design of the amplification card box is provided with a plurality of cavity combinations and a plurality of liquid path combinations, and all the liquid path combinations are connected in parallel, thereby obtaining a card box structure with multiple channels connected in parallel, preventing series flow, synchronously processing multiple sample liquids and further improving the efficiency.

The switch valve is designed in the base of the card box and can slide to change positions, so that the on-off of each liquid path in the liquid path combination can be controlled, the liquid paths in the liquid path combinations can be controlled by the switch valve at the same time, and the operation is more convenient.

The liquid path combinations are symmetrically arranged on two opposite sides of the base of the card box and are mutually independent, so that liquid mixing is prevented, the mixing or flowing time of sample liquid in each liquid path combination is consistent, and the detection accuracy is ensured.

Drawings

The described and/or additional aspects and advantages of embodiments of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings. It should be noted that the embodiments shown in the following drawings are merely exemplary for explaining the present invention, and should not be construed as limiting the present invention.

FIG. 1 shows the structure of one embodiment of an amplification cartridge.

FIG. 2 is a block diagram of the amplification cartridge of FIG. 1, showing the respective on-off valves exploded from the cartridge base and the chambers combined into four.

FIG. 3 shows 12 amplification cassettes arranged in a 4 by 12 layout.

In FIG. 4, the 24 amplification cassettes are arranged symmetrically in two rows, indicated by the horizontal 4 x 12 x 2 layout, and indicated by the vertical 24.

Fig. 5 shows 24 amplification cassettes arranged in two rows symmetrically according to a 4 x 12 x 2 layout, shown as horizontal 8 and vertical 12.

Reference numerals:

1000. a cassette base;

1101. a first fluid path; 1102. a second fluid path; 1103. a third fluid path; 1104. a fourth fluid path;

1201. a first on-off valve; 1202. a second on-off valve; 1203. a third on-off valve; 1204. a fourth switching valve; 1205. a fifth on-off valve;

1301. a first mixing switching valve; 1302. a second mixing on-off valve;

2101. a first chamber; 2102. a second chamber; 2103. a third chamber; 2104. a fourth chamber; 2105. a fifth chamber;

2201. a first mixing chamber; 2202. a second mixing chamber;

2300. a cover body;

3000. and (4) amplifying the tube.

Detailed Description

Embodiments of the invention, examples of which are illustrated in the accompanying drawings, are described in detail below with reference to fig. 1 to 5, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary only for explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it should be understood that if the terms "center", "middle", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. are used to indicate an orientation or positional relationship based on that shown in the drawings, it is only for convenience of description and simplicity of description, and it is not intended to indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. Furthermore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The utility model relates to a nucleic acid detection equipment, nucleic acid detection equipment are including amplifying the card box, and the appearance liquid of nucleic acid is discharged after schizolysis, washing and elution in the card box of amplification to detect.

Other constitution and operation of the nucleic acid detecting apparatus are well known to those skilled in the art and will not be described in detail herein, and the structure of the amplification cartridge will be described below.

The utility model relates to an amplification card box, amplification card box include cartridge base 1000 and a plurality of cavity combination, and the cavity combination includes first cavity 2101, second cavity 2102, first mixed cavity 2201, second mixed cavity 2202, third cavity 2103 and fourth cavity 2104, specifically, first cavity 2101, second cavity 2102, first mixed cavity 2201, second mixed cavity 2202, third cavity 2103 and fourth cavity 2104 are connected with cartridge base 1000 respectively. Further, a preservation solution can be stored in the first chamber 2101, a throat swab stuck with a sample solution is placed in the first chamber 2101 and mixed with the preservation solution, a washing solution can be stored in the second chamber 2102, a lysate can be stored in the second mixing chamber 2202, magnetic beads are mixed in the lysate, the third chamber 2103 is used for communicating with an air source, and an eluent can be stored in the fourth chamber 2104.

The cartridge base 1000 is provided with a plurality of liquid path combinations, and the liquid path combination is arranged in realizing the intercommunication between each cavity in the cavity combination, and specifically, the liquid path combination includes first liquid path 1101, second liquid path 1102, third liquid path 1103 and fourth liquid path 1104, and each liquid path is formed through the processing of receiving a little, is the liquid path of micron level, and each liquid path in the liquid path combination can break-make respectively to realize the intercommunication between the different cavities. Furthermore, all the liquid path combinations are connected in parallel, and all the chamber combinations form a parallel and mutually independent card box structure through the corresponding liquid path combinations, so that the structure is compact, liquid mixing is avoided, a plurality of sample liquids of nucleic acid can be processed simultaneously, and the efficiency is improved.

The first liquid path 1101 is respectively used for connecting the first chamber 2101, the second chamber 2102 and the first mixing chamber 2201, specifically, the connection between the first chamber 2101 and the second chamber 2102 and the first liquid path 1101 can be respectively switched on and off, the second liquid path 1102 is respectively used for connecting the first mixing chamber 2201 and the second mixing chamber 2202, and the first mixing chamber 2201 can be switched to communicate with the first liquid path 1101 or the second liquid path 1102. It is understood that if the first mixing chamber 2201 is switched to communicate with the second liquid passage 1102, the first mixing chamber 2201 is disconnected from the first liquid passage 1101.

Of course, in the case where the first mixing chamber 2201 is switched to communicate with the first liquid path 1101, then the first mixing chamber 2201 is disconnected from the second liquid path 1102, at which time: if the first chamber 2101 is in communication with the first fluid path 1101 and the second chamber 2102 is disconnected from the first fluid path 1101, the first chamber 2101 is in communication with the first mixing chamber 2201 and fluid may flow between the first chamber 2101 and the first mixing chamber 2201; if the second chamber 2102 is in communication with the first fluid path 1101 and the first chamber 2101 is in communication with the first fluid path 1101, the second chamber 2102 is in communication with the first mixing chamber 2201 and fluid may flow between the second chamber 2102 and the first mixing chamber 2201.

The third liquid path 1103 is used for connecting the second mixing chamber 2202, the third chamber 2103, the fourth chamber 2104 and the fourth liquid path 1104, specifically, the second mixing chamber 2202 can be switched to communicate with the second liquid path 1102 or the third liquid path 1103, the third chamber 2103 can be connected with the third liquid path 1103 to be switched on and off, the third liquid path 1103 can be switched to communicate with the fourth chamber 2104 or the fourth liquid path 1104, and the fourth liquid path 1104 is used for discharging the processed sample liquid.

It is understood that in the case where the second mixing chamber 2202 is switched to communicate with the second liquid passage 1102, the second mixing chamber 2202 is disconnected from the third liquid passage 1103, and at this time, if the first mixing chamber 2201 is switched to communicate with the second liquid passage 1102, the second mixing chamber 2202 communicates with the first mixing chamber 2201.

Of course, if the second mixing chamber 2202 is switched to communicate with the third liquid path 1103 and the third liquid path 1103 is set to communicate with the third chamber 2103, the fourth chamber 2104 or the fourth liquid path 1104, the second mixing chamber 2202 communicates with the third chamber 2103, the fourth chamber 2104 or the fourth liquid path 1104 accordingly.

Further, the first mixing chamber 2201 is provided with a first mixing plunger, the second mixing chamber 2202 is provided with a second mixing plunger, the first mixing plunger and the second mixing plunger are pushed and pulled, the second chamber 2102 is provided with a second plunger which can push the washing liquid out of the second chamber 2102, the fourth chamber 2104 is provided with a fourth plunger which can push the washing liquid out of the fourth chamber 2104.

In some examples, the second plunger, the first mixing plunger, the second mixing plunger, and the fourth plunger move in an automated manner. Specifically, the second plunger, the first mixed plunger, the second mixed plunger and the fourth plunger are driven to move through the plurality of electric telescopic structures respectively, and the upper computer is used for controlling the work of each electric telescopic structure respectively. Of course, it is also possible to replace: driven hydraulically or pneumatically.

It will be appreciated that, corresponding to the chamber combination and the liquid path combination, the amplification cartridge comprises a plurality of amplification tubes 3000, the amplification tubes 3000 have reagents therein for detecting sample liquid, the amplification tubes 3000 are connected with the cartridge base 1000, specifically, the amplification tubes 3000 are connected with the lower side of the cartridge base 1000, and the lower side of the cartridge base 1000 is provided with an interface for mounting the amplification tubes 3000.

Further, the amplification tube 3000 is communicated with a fourth liquid path 1104, and the treated sample liquid is discharged to the amplification tube 3000 through the fourth liquid path 1104. Specifically, the processed sample solution remains in the second mixing chamber, and the sample solution is discharged to the amplification tube 3000 through the third liquid path 1103 and the fourth liquid path 1104 by pushing the second mixing plunger. It will be appreciated that the sample liquid is discharged in a fixed amount by controlling the stroke of the second mixing plunger. In some examples, the second mixing plunger is configured with a stroke control element that can control the distance of movement of the second mixing plunger.

As an embodiment, a magnetic element accommodating region is disposed between the first mixing chamber 2201 and the second mixing chamber 2202. Further, the amplification cartridge includes a magnetic element near the magnetic attracting chamber of the cartridge base 1000 for attracting and fixing the magnetic beads in the liquid. Specifically, the magnetic element may be close to or far from the magnetic attraction chamber of the cartridge base 1000 in a direction perpendicular to the surface of the cartridge base 1000 in the magnetic element accommodation region.

Of course, as an alternative, it is also possible to provide: the amplification cartridge does not have a magnetic element, but the user additionally purchases the magnetic element, reducing the production cost of the amplification cartridge.

As an embodiment, the amplification cartridge comprises a plurality of switch valves, the cartridge base 1000 is provided with a plurality of recessed mounting areas, the switch valves are arranged in the mounting areas, each switch valve is used for connecting or disconnecting each connection between the liquid path combination and the chamber combination, and the connection and disconnection at the corresponding position can be switched by switching the positions of the switch valves. Specifically, the open/close valve is provided with a flow path that forms a first communication port and a second communication port on a side surface of the open/close valve.

It can be understood that each switch valve is movably connected with the cartridge base 1000, and each liquid path in the liquid path combination is controlled to be on or off synchronously through the movably connected switch valve. Further, the respective switching valves are slidably connected to the cartridge base 1000, and particularly, the respective switching valves are slid in the cartridge base 1000 in an automatic manner. In some examples, the switch valves are driven to move by a plurality of electric telescopic structures, and the upper computer is used for controlling the work of each electric telescopic structure. Of course, it is also possible to replace: driven hydraulically or pneumatically.

The first chamber 2101 is switched to be connected with or disconnected from the first liquid path 1101 by a corresponding switching valve, the switching valve corresponding to the first chamber 2101 is set as a first switching valve 1201, and a first communication port in the first switching valve 1201 is used for communicating the first chamber 2101 and a second communication port is used for communicating the first liquid path 1101. It is understood that, by changing the position of the first switching valve 1201, the first communication port and the second communication port can be shifted or aligned with the communication positions of the first chamber 2101 and the first liquid passage 1101, respectively. With reference to the drawings, when the first switching valve 1201 moves to the left, the first chamber 2101 is communicated with the first fluid path 1101; when the first switching valve 1201 moves rightward, the first chamber 2101 is disconnected from the first fluid path 1101.

The second chamber 2102 is switched to be connected with or disconnected from the first liquid path 1101 through a corresponding switch valve, the switch valve corresponding to the second chamber 2102 is set as a second switch valve 1202, and a first communication port in the second switch valve 1202 is used for communicating the second chamber 2102 and the second communication port for communicating the first liquid path 1101. It is understood that, by changing the position of the second on-off valve 1202, the first communication port and the second communication port can be displaced from or aligned with the communication positions of the second chamber 2102 and the first liquid passage 1101, respectively. With reference to the drawings, when the second on-off valve 1202 moves to the left, the second chamber 2102 communicates with the first liquid path 1101; when the second switching valve 1202 moves rightward, the second chamber 2102 is disconnected from the first liquid path 1101.

The first mixing chamber 2201 is switched to communicate with the first liquid path 1101 or the second liquid path 1102 by a corresponding on-off valve, the on-off valve corresponding to the first mixing chamber 2201 is provided as a first mixing on-off valve 1301, and a first communication port in the first mixing on-off valve 1301 is used for communicating the first mixing chamber 2201 and a second communication port is used for communicating the first liquid path 1101 or the second liquid path 1102. It is understood that, by changing the position of the first mixing switching valve 1301, the second communication port can be switched to communicate with the first liquid passage 1101 or the second liquid passage 1102, and the first communication port is kept in communication with the first mixing chamber 2201. With reference to the drawings, when the first mixing switching valve 1301 is moved to the left, the first mixing chamber 2201 is communicated with the first liquid path 1101; when the first mixing switch valve 1301 moves to the right, the first mixing chamber 2201 communicates with the second liquid passage 1102.

The second mixing chamber 2202 is switched to communicate with the second liquid passage 1102 or the third liquid passage 1103 by a corresponding on-off valve, the on-off valve corresponding to the second mixing chamber 2202 is provided as a second mixing on-off valve 1302, and the first communication port of the second mixing on-off valve 1302 is used for communicating with the second mixing chamber 2202 and the second communication port is used for communicating with the second liquid passage 1102 or the third liquid passage 1103. It is understood that, by changing the position of the second mixing switch valve 1302, the second communication port can be switched to communicate with the second liquid passage 1102 or the third liquid passage 1103, and the first communication port is kept in communication with the second mixing chamber 2202. With reference to the figure, when the second mixing switching valve 1302 moves to the left, the second mixing chamber 2202 communicates with the second liquid passage 1102; when the second mixing switching valve 1302 moves rightward, the second mixing chamber 2202 communicates with the third liquid path 1103.

The third chamber 2103 is switched to be communicated with or disconnected from the third liquid path 1103 through a corresponding switch valve, the switch valve corresponding to the third chamber 2103 is set as a third switch valve 1203, and a first communication port in the third switch valve 1203 is used for communicating the third chamber 2103 and a second communication port is used for communicating the third liquid path 1103. It is understood that, by changing the position of the third switching valve 1203, the first communication port and the second communication port can be displaced from or aligned with the communication positions of the third chamber 2103 and the third liquid passage 1103, respectively. With reference to the figure, when the third switch valve 1203 moves left, the third chamber 2103 communicates with the third liquid path 1103; when the third switching valve 1203 moves rightward, the third chamber 2103 is disconnected from the third liquid path 1103.

The fourth chamber 2104 can be switched to communicate with the fourth chamber 2104 or the third liquid path 1103 communicates with the fourth liquid path 1104 through the corresponding switch valve, the switch valve corresponding to the fourth chamber 2104 is set as the fourth switch valve 1204, a second communication port in the fourth switch valve 1204 is communicated with the third liquid path 1103, and the first communication port is used for communicating with the fourth chamber 2104. It is understood that, when the position of the fourth switching valve 1204 is changed, and the first communication port and the fourth chamber 2104 are communicated with each other in a aligned manner, the second communication port and the fourth liquid path 1104 are disconnected from each other in a misaligned manner, and the third liquid path 1103 and the fourth chamber 2104 are communicated with each other; when the first communication port and the fourth chamber 2104 are displaced and disconnected from each other, the second communication port communicates with the third liquid passage 1103 and the fourth liquid passage 1104, respectively. With reference to the drawings, when the fourth switching valve 1204 moves to the left, the third fluid path 1103 communicates with the fourth chamber 2104; when the fourth switching valve 1204 moves rightward, the third liquid path 1103 communicates with the fourth liquid path 1104.

As an embodiment, the chamber combination comprises a fifth chamber 2105, the fifth chamber 2105 is connected with the cartridge base 1000, the fifth chamber 2105 is communicated with the third liquid path 1103, and the connection between the fifth chamber 2105 and the third liquid path 1103 can be switched on and off. The fifth chamber 2105 is used for storing mineral oil or gas, and after the processed sample liquid is discharged from the amplification card box to the amplification tube 3000, in order to improve the accuracy of quantitative extraction and prevent the sample liquid from remaining in the liquid path, the sample liquid remaining in the liquid path is pushed out to the amplification tube 3000 by using mineral oil or gas.

Further, the fifth chamber 2105 is provided with a fifth plunger for pushing out the mineral oil or gas in the fifth chamber 2105. In some examples, the fifth plunger moves in the fifth chamber 2105 in an automated manner. Specifically, the fifth plunger is driven to move through the electric telescopic structure, and the upper computer is used for controlling the work of the electric telescopic structure. Of course, it is also possible to replace: driven hydraulically or pneumatically.

The fifth chamber 2105 is switched to be connected to or disconnected from the third liquid path 1103 by a corresponding switching valve, and specifically, the switching valve corresponding to the fifth chamber 2105 is set as a fifth switching valve 1205. It will be appreciated that the fifth on/off valve 1205 is movably connected to the cartridge base 1000. Specifically, the fifth on-off valve 1205 is slidably connected to the cartridge base 1000. Further, the fifth switching valve 1205 slides in the cartridge base 1000 in an automatic manner. In some examples, the fifth switch valve 1205 is driven to move by the electric telescopic structure, and the upper computer is used to control the operation of the electric telescopic structure. Of course, it is also possible to replace: driven hydraulically or pneumatically.

The fifth opening/closing valve 1205 is provided with a flow path that forms a first communication port for communicating with the fifth chamber 2105 and a second communication port for communicating with the third liquid path 1103, on a side surface of the fifth opening/closing valve 1205. It is understood that, by changing the position of the fifth on-off valve 1205, the first communication port and the second communication port can be displaced from or aligned with the communication positions of the fifth chamber 2105 and the third liquid passage 1103, respectively.

It can be understood that, under the condition that the chamber combination and the liquid path combination are respectively provided with a plurality of chambers, the switch valve is used for synchronously controlling the on-off of the liquid paths in the liquid path combinations, and the same switch valve can simultaneously control the on-off of the same liquid path in different liquid path combinations. Specifically, each ooff valve is provided with a plurality of flow paths respectively, utilizes a set of ooff valve combination can a plurality of independent card box structures of simultaneous control, realizes independent multichannel amplification, satisfies the demand that each card box structure independently handled sample liquid.

In some examples, two opposite side surfaces of the cartridge base 1000 are respectively provided with a liquid path combination, and the first liquid path 1101, the second liquid path 1102 and the third liquid path 1103 are respectively symmetrically arranged on two opposite sides of the cartridge base 1000, so that on one hand, the on-off valve can conveniently control the on-off of a plurality of liquid path combinations at the same time, and on the other hand, the time for mixing or flowing samples in each liquid path combination is consistent, so that the transfer amount and the mixing degree of each sample can be ensured to be consistent, and the detection accuracy can be ensured. It can be understood that, on the switch valve, the second communication ports are located on two opposite side surfaces of the switch valve; and on the upper side surface of the switch valve, the first communication ports are arranged in a row. In this case, the chambers corresponding to the respective on-off valves are arranged in a row on the cartridge base 1000. Specifically, the first chambers 2101, the first mixing chambers 2201, and the second mixing chambers 2202 are arranged in a line on the cartridge base 1000, and the flow paths on the first switching valve 1201, the first mixing switching valve 1301, and the second mixing switching valve 1302 are arranged in a line.

Of course, as an alternative, it is also possible to provide: on the upper side of the switch valves, the first communication ports are arranged in two rows, in which case the chambers corresponding to the switch valves are arranged in two rows on the cartridge base 1000. Specifically, the second chambers 2102, the third chambers 2103, the fourth chambers 2104 and the fifth chambers 2105 are arranged in two rows on the cartridge base 1000, and the flow paths of the second switching valve 1202, the third switching valve 1203, the fourth switching valve 1204 and the fifth switching valve 1205 are arranged in two rows.

It is understood that the number of amplification tubes 3000 corresponds to the number of chamber combinations and liquid path combinations. Further, the amplification tubes 3000 are sequentially spaced in the axial direction of the cartridge base 1000, and the amplification tubes 3000 are arranged in a row. In this case, the length of the fourth liquid path 1104 connected to the amplification tube 3000 is not uniform, and the sample liquid in the liquid path can be discharged by the mineral oil or gas in the fifth chamber 2105 in order to eliminate quantitative variations in the liquid sampling.

With reference to the drawings, four chamber combinations and four liquid path combinations are provided to form a quadruplet cartridge, and correspondingly, four amplification tubes 3000 are provided. Further, two liquid path combinations are respectively arranged on two opposite side surfaces of the cartridge base 1000. Specifically, four first chambers 2101, four first mixing chambers 2201, and four second mixing chambers 2202 are arranged in a row on the cartridge base 1000, respectively, and accordingly, the first communication ports of the first switching valve 1201, the first mixing switching valve 1301, and the second mixing switching valve 1302 are arranged in a row on the upper side surface, respectively. The four second chambers 2102, the four third chambers 2103, the four fourth valves and the four fifth chambers 2105 are arranged in two rows on the cartridge base 1000, and the respective first communication ports of the second switching valve 1202, the third switching valve 1203, the fourth switching valve 1204 and the fifth switching valve 1205 are arranged in two rows on the upper side.

It can be understood that a single amplification cartridge can process four nucleic acid samples, and in the nucleic acid detection device, a plurality of quadruplet cartridges are combined, so that the automatic detection under the closed condition of the high-throughput cartridge can be conveniently realized, the detection quantity and the detection speed are improved, and the high-throughput detection is realized.

As an embodiment, the amplification cartridge comprises a cover 2300, and the cover 2300 is connected to the first chamber 2101 and the connection is detachable. It will be appreciated that to maintain the air pressure in the first chamber 2101 in equilibrium, the first cover 2300 is connected to a source of air, which is provided as an atmosphere or gas cylinder, and the cover 2300 is provided. Further, a filter element is disposed in the cover 2300 to filter the gas and improve the gas purity.

It is understood that, in the case where the first chamber 2101 is provided in plurality, accordingly, the cover 2300 is provided in plurality. Further, the covers 2300 are integrally joined to open and close the covers 2300 at the same time. With reference to the drawings, the cover bodies 2300 are integrally connected to the amplification cassette. As an alternative, it is of course also possible to provide: each cover 2300 is separately connected to each first chamber 2101.

As an embodiment, the third chamber 2103 is provided with a filter element, the air source connected to the third chamber 2103 is an atmosphere or a gas bottle, and the filter element is arranged at the opening of the third chamber 2103 to filter the gas, so that the gas purity is improved.

The following will supplement the description of the nucleic acid detecting apparatus with respect to the structure of the amplification cartridge.

In one embodiment, the number of the amplification cartridges is 12, the amplification cartridges have four chamber combinations and four liquid path combinations, the amplification tubes 3000 in the amplification cartridges are arranged in a 4 × 12 array, and the amplification cartridges are arranged in a row in the nucleic acid detecting apparatus. In this case, the cartridge is reasonable in layout, is in butt joint with a sample plate of the PCR instrument, and can realize detection of 48 independent samples.

In one embodiment, the number of the amplification cartridges is 24, the amplification cartridges have four chamber combinations and four liquid path combinations, the amplification tubes 3000 in the amplification cartridges are arranged in a 4 × 12 × 2 array, and the amplification cartridges are symmetrically arranged in two rows in the nucleic acid detecting apparatus. In this case, detection of 98 independent samples can be achieved.

The symmetrical arrangement mode has two types: one overall layout is that 3000 amplification tubes are 8 in the horizontal direction and 12 in the vertical direction; another overall layout is that the amplification tubes 3000 are 4 in the horizontal direction and 24 in the vertical direction.

Furthermore, by adding 24 amplification cassettes in any of the above symmetrical manners, detection of 192 independent samples can be achieved.

Based on the above detailed description of the structure of the amplification cartridge, the following description of the amplification method for processing a sample solution using the amplification cartridge is provided, and it should be noted that the following description is illustrative and not intended to limit the present invention in particular.

The amplification method adopts an automatic mode to control the on-off of the liquid paths and the chambers at all positions in the amplification card box, and in the flow of the amplification method, all liquid path components are connected in parallel and can simultaneously control the on-off of the same liquid path in all liquid path combinations by moving the switch valve.

The amplification method comprises the following steps: s1, adding a sample solution; s2, cracking and mixing, magnetically attracting, and discharging waste liquid; s3, removing liquid path residues; s4, washing, magnetically attracting and discharging waste liquid; s5, eluting and magnetically attracting; and S6, quantitatively discharging.

In step S1, the sample solution to be processed is put into the first chamber 2101 and premixed in the first mixing chamber 2201.

The throat swab is inserted into the first cavity 2101, the sample liquid adhered to the throat swab is mixed with the preservation liquid, and the cover 2300 is closed with the first cavity 2101.

The first switching valve 1201 moves to the left to communicate the first chamber 2101 with the first liquid path 1101; the first mixing switching valve 1301 is shifted left to communicate the first mixing chamber 2201 with the first liquid path 1101; the sample fluid is transferred to the first mixing chamber 2201 by pushing or pulling the first mixing plunger, which can facilitate mixing of the sample fluid and the preservation fluid.

In step S2, the lysis solution is mixed with the sample solution, the nucleic acid molecules in the sample solution are magnetically adsorbed, and the waste solution after lysis is transferred to the first chamber 2101.

The first mixing switching valve 1301 moves to the right, and the first mixing chamber 2201 is switched to communicate with the second liquid passage 1102; the second mixing switching valve 1302 moves to the left, and the second mixing chamber 2202 communicates with the second liquid passage 1102; the sample solution and the lysate are mixed by pushing and pulling the first mixing plunger and the second mixing plunger, and the sample solution mixed lysate flows back and forth between the first mixing chamber 2201 and the second mixing chamber 2202, thereby sufficiently performing lysis mixing.

The magnetic element is close to the magnetic suction chamber of the cartridge base 1000, and the magnetic element moves downwards in the magnetic element accommodating area to be close to the magnetic suction chamber of the cartridge base 1000; when the second mixing plunger is pushed, the liquid is transferred from the second mixing chamber 2202 to the first mixing chamber 2201, and the magnetic element adsorbs and fixes the magnetic beads during the flowing process of the sample liquid. It can be understood that the mixing speed is reduced during the process of adsorbing the fixed magnetic beads by the magnetic element.

The first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be communicated with the first liquid path 1101; the first switching valve 1201 moves to the left, and the first chamber 2101 is communicated with the first liquid path 1101; the lysed waste is transferred to the first chamber 2101 by pushing the first mixing plunger.

In step S3, the gas in the third chamber 2103 is used to clean the residual liquid in the first liquid path 1101 and the second liquid path 1102, and the residual liquid is transferred to the first chamber 2101.

The third switching valve 1203 moves left, and the third chamber 2103 is communicated with the third liquid path 1103; the second mixing switching valve 1302 moves to the right, and the second mixing chamber 2202 is switched to communicate with the third liquid path 1103; drawing gas from the third chamber 2103 to the second mixing chamber 2202 through the second mixing plunger; the third switching valve 1203 moves rightward, and the third chamber 2103 is disconnected from the third liquid path 1103.

The second mixing switching valve 1302 moves left and the second mixing chamber 2202 is switched to communicate with the second liquid passage 1102; the first mixing switching valve 1301 moves to the right, and the first mixing chamber 2201 is switched to be communicated with the second liquid path 1102; by pushing the second mixing plunger, gas is transferred from the second mixing chamber 2202 to the first mixing chamber 2201, which can clean up the residual liquid in the second liquid path 1102.

The first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be communicated with the first liquid path 1101; the first switching valve 1201 moves leftwards, and the first chamber 2101 is communicated with the first liquid path 1101; by pushing the first mixing plunger, gas is introduced from the first mixing chamber 2201 into the first chamber 2101, the gas can clean up the residual liquid in the first liquid path 1101.

In step S4, the nucleic acid molecules adsorbed by the magnetic beads are washed, and the waste solution after washing is transferred to the first chamber 2101.

The second switching valve 1202 moves to the left, and the second chamber 2102 communicates with the first liquid path 1101; the first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be communicated with the first liquid path 1101; the first plunger is pushed and the wash solution is transferred from the second chamber 2102 to the first mixing chamber 2201 by pulling the first mixing plunger.

The first mixing switching valve 1301 moves to the right, and the first mixing chamber 2201 is switched to be communicated with the second liquid path 1102; the second mixing switching valve 1302 moves to the left, and the second mixing chamber 2202 is switched to communicate with the second liquid passage 1102; the cleaning solution is caused to flow between the first mixing chamber 2201 and the second mixing chamber 2202 by pushing and pulling the first mixing plunger and the second mixing plunger. It will be appreciated that in this process, the magnetic element is remote from the magnetically attracted chamber of the cartridge base 1000 and the magnetic beads are suspended in the wash solution to facilitate processing of the nucleic acid molecules by the wash solution.

The magnetic element is close to the magnetic suction chamber of the cartridge base 1000, and the magnetic element moves downwards in the magnetic element accommodating area to be close to the magnetic suction chamber of the cartridge base 1000; the second mixing plunger is pushed, the first mixing plunger is pulled, the washing solution is transferred from the second mixing chamber 2202 to the first mixing chamber 2201, and in the flowing process of the liquid, the magnetic elements adsorb and fix the magnetic beads. It can be understood that the mixing speed is reduced during the process of adsorbing the fixed magnetic beads by the magnetic element.

The first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be communicated with the first liquid path 1101; the first switching valve 1201 moves to the left, and the first chamber 2101 is communicated with the first liquid path 1101; the washed waste is transferred to the first chamber 2101 by pushing the first mixing plunger.

In step S5, the nucleic acid molecules adsorbed by the magnetic beads are eluted, and the sample solution obtained by the elution is stored in the second mixing chamber 2202.

The second mixing switching valve 1302 moves to the right, and the second mixing chamber 2202 is switched to communicate with the third liquid path 1103; the fourth switching valve 1204 is moved leftwards, and the fourth chamber 2104 is switched to be communicated with the third liquid path 1103; pushing the fourth plunger and pulling the second mixing plunger, eluent is transferred from the fourth chamber 2104 to the second mixing chamber 2202.

The second mixing switching valve 1302 moves left and the second mixing chamber 2202 is switched to communicate with the second liquid passage 1102; the first mixing switching valve 1301 moves to the right, and the first mixing chamber 2201 is switched to be communicated with the second liquid path 1102; eluent is flowed between the first mixing chamber 2201 and the second mixing chamber 2202 by pushing and pulling the first mixing plunger and the second mixing plunger.

During the elution process, the magnetic element is far away from the magnetic suction chamber of the cartridge base 1000, and the magnetic beads are suspended in the sample solution; after the elution is completed, the magnetic element is close to the magnetic attraction chamber of the cartridge base 1000, the magnetic beads are adsorbed and fixed in the magnetic attraction chamber, and the sample solution after the eluent treatment is transferred to the second mixing chamber 2202.

In step S6, the sample solution obtained after elution is quantitatively discharged to the amplification tube 3000, and the sample solution remaining in the third liquid path 1103 and the fourth liquid path 1104 is cleaned up to the amplification tube 3000.

The second mixing switching valve 1302 moves to the right, and the second mixing chamber 2202 is switched to be communicated with the third liquid path 1103; the fourth switching valve 1204 moves left, and the fourth chamber 2104 is switched to communicate with the third liquid path 1103; the gas remaining in the third liquid path 1103 is transferred to the fourth chamber 2104 by pushing the second mixing plunger to fill the third liquid path 1103 with the sample liquid.

The fourth switching valve 1204 is moved rightward to switch the fourth liquid path 1104 to communicate with the third liquid path 1103, and a predetermined amount of sample liquid is extracted by pushing the second mixing plunger, and the sample liquid is discharged to the amplification tube 3000 via the third liquid path 1103 and the fourth liquid path 1104. It will be appreciated that by setting the distance of travel of the second mixing plunger, a quantitative discharge can be achieved.

The second mixing switching valve 1302 moves leftward, and the second mixing chamber 2202 is switched to communicate with the second liquid passage 1102, so that the second mixing chamber 2202 is disconnected from the third liquid passage 1103; the fifth switch valve 1205 moves to the left, and the fifth chamber 2105 is communicated with the third liquid path 1103; the fifth plunger is pushed, mineral oil or gas in the fifth chamber 2105 fills the third liquid path 1103 and the fourth liquid path 1104, and sample liquid in the third liquid path 1103 and the fourth liquid path 1104 is discharged, so that accurate quantitative liquid taking is realized.

It is understood that, during the washing in step S4, it may alternatively be designed that: the magnetic element is close to the magnetic attraction chamber of the cartridge base 1000, and the magnetic element keeps the magnetic beads attracted and fixed during the washing process.

In the description herein, references to the terms "one embodiment," "some examples," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" or the like, if any, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The embodiments of the present invention have been described in detail with reference to the drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the gist of the present invention within the knowledge of those skilled in the art.

In the description of the present invention, if the patent names "and" appear, "they refer to the relationship of" and "rather than" or ". For example, the patent names "a and B" illustrate that the claimed invention is: the technical scheme with the subject name of A and the technical scheme with the subject name of B.

Claims (13)

1. An amplification cartridge, comprising: including card box base (1000), a plurality of amplification pipe (3000) and a plurality of chamber combination, the chamber combination with card box base (1000) are connected, amplification pipe (3000) with card box base (1000) are connected, the chamber combination includes

A first chamber (2101) in which a preservation solution can be stored, the first chamber (2101) being connected to the cartridge base (1000);

a second chamber (2102), the second chamber (2102) being connected to the cartridge base (1000), a washing liquid being storable in the second chamber (2102), the second chamber (2102) being provided with a second plunger;

a first mixing chamber (2201), the first mixing chamber (2201) being connected with the cartridge base (1000), the first mixing chamber (2201) being provided with a first mixing plunger;

a second mixing chamber (2202), the second mixing chamber (2202) being connected with the cartridge base (1000), a lysate being storable in the second mixing chamber (2202), the second mixing chamber (2202) being provided with a second mixing plunger;

a third chamber (2103), wherein the third chamber (2103) is connected with the cartridge base (1000), and the third chamber (2103) is used for being communicated with a gas source;

a fourth chamber (2104) connected to the cartridge base (1000), an eluent being storable in the fourth chamber (2104), the fourth chamber (2104) being provided with a fourth plunger;

the cartridge base (1000) is provided with a plurality of liquid path combinations, each liquid path combination comprises a first liquid path (1101), a second liquid path (1102), a third liquid path (1103) and a fourth liquid path (1104), the first liquid path (1101) is respectively used for connecting the first chamber (2101), the second chamber (2102) and the first mixing chamber (2201), the second liquid path (1102) is respectively used for connecting the first mixing chamber (2201) and the second mixing chamber (2202), the third liquid path (1103) is respectively used for connecting the second mixing chamber (2202), the third chamber (2103) and the fourth chamber (2104), the fourth liquid path (1104) is connected with the amplification tube (3000), and the liquid paths in the liquid path combinations are respectively and synchronously controlled to be switched on and switched off through movably connected switching valves.

2. The amplification cartridge of claim 1, wherein: a magnetic element accommodating area is arranged between the first mixing chamber (2201) and the second mixing chamber (2202).

3. The amplification cartridge of claim 2, wherein: the amplification cartridge comprises a magnetic element that is movable in a direction perpendicular to the surface of the cartridge base (1000) in the magnetic element receiving region toward or away from the magnetic attraction chamber of the cartridge base (1000).

4. The amplification cartridge of claim 3, wherein: the chamber combination comprises a fifth chamber (2105), wherein the fifth chamber (2105) is connected with the cartridge base (1000), the fifth chamber (2105) is used for storing mineral oil or gas, a fifth plunger is arranged in the fifth chamber (2105), the fifth chamber (2105) is communicated with the third liquid path (1103), and the connection between the fifth chamber (2105) and the third liquid path (1103) can be switched on and off.

5. The amplification cartridge of claim 4, wherein: each switch valve is respectively connected with the cartridge base (1000) in a sliding manner.

6. The amplification cartridge of claim 5, wherein: the two opposite side surfaces of the cartridge base (1000) are respectively provided with the liquid path combination, the first liquid path (1101), the second liquid path (1102) and the third liquid path (1103) are symmetrically arranged on two opposite sides of the cartridge base (1000), a plurality of flow paths are arranged in the switch valve, first communication ports and second communication ports are formed in the side surfaces of the switch valve through the flow paths, the first communication ports are arranged in a row or two rows on the upper side surface of the switch valve, and the second communication ports are respectively located on two opposite side surfaces of the switch valve.

7. The amplification cartridge of claim 1, wherein: the amplification cartridge comprises a plurality of covers (2300), and the covers (2300) are connected with the first chamber (2101) in a split or integrated manner.

8. The amplification cartridge of claim 7, wherein: a filter element is arranged in the cover body (2300).

9. The amplification cartridge of any one of claims 1 to 8, wherein: the third chamber (2103) is provided with a filter element.

10. The amplification cartridge of any one of claims 1 to 6, wherein: the second mixing plunger is provided with a stroke control element that controls the distance of movement of the second mixing plunger.

11. The amplification cartridge of any one of claims 4 to 6, wherein: the amplification cartridge includes four of the chamber combinations, four of the fluid path combinations, and four of the amplification tubes (3000).

12. A nucleic acid detecting apparatus characterized in that: comprising an amplification cartridge according to any one of claims 1 to 11.

13. A nucleic acid detecting apparatus characterized in that: the amplification cartridge of claim 11, wherein the amplification cartridge is provided in 12, the amplification tubes (3000) in the amplification cartridge are arranged in a 4 x 12 array, and each amplification cartridge is arranged in a row in the nucleic acid detecting apparatus; or the amplification cartridges are arranged in 24, the amplification tubes (3000) in the amplification cartridges are distributed according to a 4 x 12 x 2 array, and the amplification cartridges are symmetrically arranged in two rows in the nucleic acid detecting apparatus.

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