CN217781174U - Disposable mechanical tidal cell culture device - Google Patents
Disposable mechanical tidal cell culture device Download PDFInfo
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- CN217781174U CN217781174U CN202221733867.4U CN202221733867U CN217781174U CN 217781174 U CN217781174 U CN 217781174U CN 202221733867 U CN202221733867 U CN 202221733867U CN 217781174 U CN217781174 U CN 217781174U
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Abstract
The utility model provides a disposable mechanical tidal cell culture device, which comprises a liquid storage bag, a lifting device, a tank body, a carrier and a cell carrier, wherein the carrier is arranged in the liquid storage bag and carries the cell carrier; the upper part of the liquid storage bag is provided with a vent hole; the lifting device comprises a lifting tray, the tray is positioned below the liquid storage bag and arranged in the tank body, and the tray can drive the bottom of the liquid storage bag to lift; the tank body is provided with a first connecting piece, and the liquid storage bag is provided with a second connecting piece; the liquid storage bag is arranged in the tank body, and the first connecting piece is connected with the second connecting piece; the elevating gear repeatedly usable that this technical scheme provided, aseptic effect has both been guaranteed and the cost is reduced as the stock solution container to disposable stock solution bag, and the stock solution bag is placed in jar internal assurance stock solution bag and is not destroyed, has improved the security, is connected stock solution bag and jar body through first connecting piece and second connecting piece, makes getting of stock solution bag put more convenient.
Description
Technical Field
The utility model relates to a cell culture field specifically relates to a disposable mechanical type morning and evening tides cell culture device.
Background
With the rapid development of the cell therapy industry, the use of tidal cell culture devices is widely used. The liquid storage container of the existing tidal cell culture device comprises a first cavity and a second cavity, wherein the first cavity is communicated with the second cavity, the first cavity can stretch up and down, cell culture solution is stored in the first cavity, and the second cavity is arranged above the first cavity; the device is placed on elevating gear, and when elevating gear upward movement, the first cavity of telescopic is compressed, and the inner space of first cavity diminishes, and the cell culture liquid in the first cavity gets into the second cavity, with the cell carrier submergence in the second cavity, and first cavity extends when elevating gear downstream, and the inner space grow of first cavity, cell culture liquid from the second cavity backward flow to first cavity inside, the cell carrier in the second cavity exposes from the cell culture liquid. The existing tidal cell culture device is high in production cost, needs to be cleaned and disinfected after being used every time, increases the workload of a user, and is difficult to ensure that the sterile cell culture device meets the sterile requirement after being disinfected, so that a disposable tidal cell culture device with low cost is needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a disposable mechanical type morning and evening tides cell culture device that disposable and stock solution container low in production cost.
In order to achieve the above purpose, the utility model provides a disposable mechanical tidal cell culture device, which comprises a liquid storage container, wherein the upper part of the liquid storage container is provided with an air vent; the lifting device comprises a lifting tray, the tray is positioned below the liquid storage container, and the tray can drive the liquid storage container to lift; the tank body is provided with a first connecting piece; the liquid storage container is a liquid storage bag, the liquid storage bag is made of flexible materials, and the liquid storage bag is provided with a second connecting piece; the liquid storage bag is arranged in the tank body, and the first connecting piece is connected with the second connecting piece; the tray is arranged in the tank body.
Therefore, the production cost of the liquid storage container is reduced as the liquid storage container is made of the liquid storage bag, the disposable liquid storage bag avoids the situation that the sterile environment of the liquid storage container does not reach the standard due to the fact that disinfection is not in place, the liquid storage bag is arranged in the tank body, the tank body can protect the liquid storage bag in all directions, the liquid storage bag is prevented from being punctured and damaged, the lifting device drives the liquid storage bag made of the flexible material to lift, the lifting of the liquid level of cell culture liquid in the liquid storage bag is achieved, and the tide effect is simulated; through first connecting piece and second connecting piece with the stock solution bag with jar body coupling, guaranteed the stability of stock solution bag at jar internal, also make things convenient for the stock solution bag to take out from jar internal.
According to the scheme, when the liquid storage bag is in a liquid storage state, the liquid storage part of the liquid storage bag is abutted to the inner wall surface of the tank body, and/or the tray supports the liquid storage bag.
Therefore, the liquid storage part of the liquid storage bag is abutted against the inner wall surface of the tank body, and the inner wall surface of the tank body can help the liquid storage bag bear the pressure of part of liquid in the liquid storage bag to the liquid storage bag, so that the reliability of the liquid storage bag is improved, and the liquid storage bag is prevented from being broken due to excessive liquid storage; the tray supports the liquid storage bag, so that most of force borne by the bottom of the liquid storage bag can be reduced, and the safety and the stability of the liquid storage bag are improved.
According to the scheme, the top of the tank body is provided with an opening; the tank body further comprises a top cover, and the top cover is detachably arranged on the opening.
Therefore, the top of the tank body is provided with an opening to facilitate taking and placing of the liquid storage bag, the detachable top cover can protect the liquid storage bag from the upper part of the tank body, and the tank cover top cover can be selected according to actual demands.
According to the scheme, the first connecting piece is arranged on the top cover; the first connecting piece is detachably connected with the second connecting piece; the liquid storage bag is hung on the top cover.
Therefore, the liquid storage bag is hung in the tank body through the first connecting piece on the top cover, the liquid storage bag and the top cover can be installed in place through the lifting device, the first connecting piece and the second connecting piece can be detachably connected, and the liquid storage bag is convenient to replace.
According to a further scheme, the lifting device further comprises a driving unit, and the driving unit drives the tray; the driving unit is arranged outside the tank body, and the tray is arranged inside the tank body.
Therefore, the tray is arranged in the tank body to drive the bottom of the liquid storage bag to lift, and the driving unit is arranged outside the tank body to facilitate overhauling and maintenance.
According to the scheme, the liquid storage bag is further provided with a water outlet which is arranged at the bottom of the liquid storage bag.
Therefore, after liquid in the liquid storage bag needs to be replaced or cell culture is finished, the water outlet can conveniently discharge the liquid in the liquid storage bag.
According to the scheme, the bottom of the liquid storage bag comprises a first cambered surface protruding downwards, and the water outlet is formed in the lowest point of the first cambered surface.
Therefore, the first cambered surface protruding downwards is matched with the water outlet arranged at the lowest point of the first cambered surface, so that the liquid in the liquid storage bag can be discharged to the maximum extent.
According to the scheme, the tray is further provided with a second cambered surface which is concave downwards, and the second cambered surface is matched with the first cambered surface.
Therefore, the cooperation of the second cambered surface and the first cambered surface can enable the tray to better share the pressure at the bottom of the liquid storage bag.
According to the scheme, the disposable mechanical tidal cell culture device further comprises a carrier and a liquid inlet pipe, wherein the carrier carries cell carriers; the liquid inlet pipe is communicated with the inside and the outside of the liquid storage bag; the liquid inlet pipe is connected with the carrier, the carrier is hung on the liquid inlet pipe, and the carrier is avoided from the pipe orifice of the liquid inlet pipe.
It is thus clear that, hang the carrier in stock solution container through the one end of feed liquor pipe, the feed liquor pipe both played the effect of carrying cell culture liquid and cell in the stock solution container, can play the effect of fixed carrier again, has consequently saved the consumptive material of fixed carrier, has reduced manufacturing cost, and the size of carrier can carry out the adaptability adjustment according to the user demand, satisfies multiple user demand.
According to a further aspect of the above solution, the carrier is a net structure or a porous structure.
It follows that the mesh or porous structure allows the cells to be in sufficient contact with the cell culture fluid in the reservoir.
Drawings
FIG. 1 is a schematic view of a disposable mechanical tidal cell culture device according to an embodiment of the present invention, in which a tray is in an initial position.
FIG. 2 is a schematic view showing the state where the bottom of the liquid storage bag is supported by the tray of the embodiment of the disposable mechanical tidal cell culture apparatus of the present invention.
The present invention will be further explained with reference to the drawings and examples.
Detailed Description
Referring to fig. 1, the disposable mechanical tidal cell culture device comprises a liquid storage bag 1, a tank 2, a carrier 3, a lifting device and a cell carrier 4. Wherein, a certain amount of cell culture solution 14 is filled in the liquid storage bag 1 in the using state, the carrier 3 is arranged at the upper part of the liquid storage bag 1, the carrier 3 is loaded with the cell carrier 4, and the cell carrier 4 is provided with cells to be cultured; the liquid storage bag 1 is arranged in the tank body 2, the lifting device drives the bottom of the liquid storage bag 1 to ascend and descend, when the lifting device drives the bottom of the liquid storage bag 1 to ascend, the liquid level of the cell culture solution 14 in the liquid storage bag 1 ascends, the carrier 3 is below the liquid level of the cell culture solution 14, when the lifting device descends, the liquid storage bag 1 naturally falls along with the lifting device under the action of gravity, the liquid level of the cell culture solution 14 descends, and the carrier 3 is above the liquid level of the cell culture solution 14.
The top of the liquid storage bag 1 is provided with two liquid inlets 151, the two liquid inlets 151 are symmetrically arranged, two-way liquid inlet connectors 152 are welded on the liquid inlets 151, the two-way liquid inlet connectors 152 are respectively connected with one ends of two second liquid inlet pipes 602 in the liquid storage bag 1, and the second liquid inlet pipes 602 are platinum vulcanized silicone pipes; in the outside of stock solution bag 1, first feed liquor pipe 601 is connected respectively to two-way feed liquor connectors 152, and platinum vulcanized silicone tube is chooseed for use to first feed liquor pipe 601, respectively is provided with a first pipe clamp 131 on two first feed liquor pipes 601, and when first pipe clamp 131 loosened, the first feed liquor pipe 601 of accessible was to filling cell culture solution in the stock solution bag 1, and when first pipe clamp 131 pressed from both sides tightly, the route between first feed liquor pipe 601 and the stock solution bag 1 was closed.
The top of stock solution bag 1 still is provided with vent 181, vent 181 department welding has vent connector 182, vent 181 is connected with the one end of blast pipe 7 through vent connector 182, the other end of blast pipe 7 is connected with the circular air cleaner 121 of first degerming, the circular air cleaner 121 of first degerming prevents that pollutants such as bacterium from getting into the internal environment of stock solution bag 1 internal contamination stock solution bag 1 from blast pipe 7, still be provided with third pipe clamp 133 on the blast pipe 7, the break-make of blast pipe 7 pipeline can be controlled to third pipe clamp 133. The top of stock solution bag 1 still symmetry is provided with 2 second connecting pieces 26, and second connecting piece 26 is the link of welding at the top of stock solution bag 1.
The bottom of the liquid storage bag 1 is provided with an air inlet 191 and a liquid outlet 201. The air inlet 191 is welded with an air inlet connector 192, the air inlet connector 192 is communicated with the inner space and the outer space of the liquid storage bag 1, the bubble generator 5 is arranged inside the liquid storage bag 1 and is connected with one end of the air inlet connector 192, the other end of the air inlet connector 192 is connected with one end of the air inlet pipe 8, the other end of the air inlet pipe 8 is connected with a second degerming circular air filter 191, the air inlet pipe 8 can convey various gases required by cell culture to the inside of the liquid storage bag 1 through the bubble generator 5, the various gases conveyed to the inside of the liquid storage bag 1 by the bubble generator 5 are decomposed into micro bubbles, the second degerming circular air filter 191 can filter the gases entering the liquid storage bag, the air inlet pipe 8 is provided with a fourth pipe clamp 134, the fourth pipe clamp 134 can control the on-off of the air inlet pipe 8, and the air inlet pipe 8 is made of platinum vulcanized silicone rubber; the liquid discharge port 201 is welded with a liquid discharge connector 202, the liquid discharge connector 202 is connected with a liquid discharge pipe 11 outside the liquid storage bag 1, a second pipe clamp 132 is arranged on the liquid discharge pipe 11, the second pipe clamp 132 can control the on-off of the liquid discharge pipe 11, the cell culture solution 14 in the liquid storage bag 1 can be discharged by opening the second pipe clamp 132, preferably, the bottom of the liquid storage bag 1 is set to be in the shape of a first cambered surface 21 protruding downwards, and the liquid discharge port 201 is arranged at the bottommost part of the first cambered surface 21 of the liquid storage bag 1.
The carrier 3 is arranged at the upper position inside the liquid storage bag 1, and a certain distance is reserved between the carrier 3 and the bottom of the liquid storage bag 1; the carrier 3 is a container with a net structure or a porous structure and has an internal space, preferably, the carrier 3 is a medical plastic net bag, the cell carrier 4 is a sheet-shaped carrier, and the cell carrier 4 is placed in the internal space of the carrier 3; the lower ends of the two second liquid inlet pipes 602 are respectively connected with two sides of the carrier 3, the pipe orifices of the two second liquid inlet pipes 602 face to avoid the carrier 3 and face to the bottom of the liquid storage bag 1, the carrier 3 is hung inside the liquid storage bag 1 by the two second liquid inlet pipes 602, and the cell culture solution 14 filled from the second liquid inlet pipes 602 avoids the carrier 3 and directly flows into the liquid storage bag 1.
An opening 23 is formed in the top of the tank body 2, a top cover 24 is arranged on the opening 23, the top cover 24 is detachably mounted on the opening 23, a first connecting piece 25 is arranged on the lower surface of the top cover 24, and the first connecting piece 25 is a hook; the bottom of the tank body 2 is provided with a third cambered surface which is sunken downwards. The bottom of the tank 2 is further provided with a first through hole 27, a second through hole 28 and a third through hole 22. The drain pipe 11 extends from the first through hole 27 to the outside of the tank 2; the intake pipe 8 extends to the outside of the can 2 through the third through hole 22. The bottom of the tank body 2 is provided with a support, and the support supports the tank body 2, so that a certain distance is reserved between the bottom of the tank body 2 and the ground.
The lifting device comprises a tray 10 and a cylinder 9 which are connected in sequence, and an expansion link 901 is arranged at the upper end of the cylinder 9. The tray 10 is provided with a second cambered surface 17 which is concave downwards, and the second cambered surface 17 is matched with the first cambered surface 21; the tray 10 is arranged inside the tank 2, the cylinder 9 is arranged outside the tank 2, and the expansion rod 901 penetrates through the second through hole 28 to connect the tray 10 and the cylinder 9 below the tank 2. The cylinder 9 is a driving unit of this embodiment, the cylinder 9 controls the telescopic rod 901 to perform telescopic motion in the vertical direction, and the telescopic rod 901 drives the tray 10 to perform lifting motion in the tank body 2.
Referring to fig. 1 and 2, when cells are cultured by using the disposable mechanical tidal cell culture device of the present embodiment, first, a liquid storage bag is hung in the tank 2 through the first connector 25 and the second connector 26; then, the second liquid inlet pipe 602 is used for filling the cell culture solution 14 and the cells into the liquid storage bag 1, when the carrier 3 is immersed in the cell culture solution 14, the filling of the cell culture solution 14 into the liquid storage bag 1 is stopped, and the first liquid inlet pipe 601 is clamped by using the first pipe clamp 131; at the moment, the bottom of the liquid storage bag 1 is abutted against the upper surface of the tray 10, the second cambered surface 17 is matched with the first cambered surface 21, and the tray 10 supports the liquid storage bag 1; the side wall surface of the liquid storage bag 1 abuts against the inner wall surface of the tank body 2. Meanwhile, gas required by cell culture such as oxygen, carbon dioxide and the like is introduced into the liquid storage bag 1 through the air inlet pipe 8, and the introduced gas is subjected to fine treatment on the gas through the micropore exhaust distribution head on the bubble generator 3 to form tiny bubbles, so that the growth of cells is facilitated. In the cell culture process, the tray 10 is located at the position shown in fig. 1 in the initial state, the air cylinder 9 drives the telescopic rod 901 to stretch, the telescopic rod 901 drives the tray 10 to do lifting motion in the cylinder body 2, when the tray 10 drives the bottom of the liquid storage bag 1 to lift, the liquid level of the cell culture solution 14 in the liquid storage bag 1 rises until the tray 10 moves to the position shown in fig. 2, and the carrier 3 is below the liquid level of the cell culture solution 14; when the tray 10 descends, the liquid storage bag 1 naturally falls back along with the tray 10 under the action of gravity, and the liquid level of the cell culture solution 14 descends, so that the cell tide type culture is realized; when the tray 10 is raised, a part of the gas in the liquid storage bag 1 is discharged from the gas discharge pipe 7, and when the tray 10 is lowered, the gas is replenished into the liquid storage bag 1 through the gas inlet pipe 8, so that the air pressure balance in the liquid storage bag 1 is maintained within a certain range. In summary, the disposable mechanical tidal cell culture device provided in this embodiment simulates a tidal cell culture environment, the production cost is reduced by using the liquid storage bag 1 as a liquid storage container, the tank 2 has a good protection effect on the liquid storage bag 1 disposed therein, and the inner wall of the tank 2 and the upper surface of the tray 10 share most of the pressure of the liquid in the liquid storage bag 1 on the bag body of the liquid storage bag 1; one end of the second liquid inlet pipe 602 hangs the carrier 3 in the liquid storage bag 1, so that the material for fixing the carrier 3 is saved; the mouth of pipe of second feed liquor pipe 602 is towards carrier 3, and cell culture solution 14 gets into the cell carrier 4 in the stock solution bag 1 the very first time after and waits the cell of cultivateing in the contact carrier 3, has improved the efficiency that cell absorbed cell culture solution 14, and cylinder 9 sets up in the outside of jar body 2 and has made things convenient for the maintenance of cylinder 9.
In some embodiments, the cells are adsorbed directly onto the carrier.
In some embodiments, the cells are adsorbed directly onto the inner wall of the reservoir bag.
Claims (10)
1. A disposable mechanical tidal cell culture device comprising:
a reservoir;
the vent hole is positioned at the upper part of the liquid storage container;
the lifting device comprises a lifting tray, the lifting tray is positioned below the liquid storage container, and the lifting tray can drive the liquid storage container to lift;
it is characterized in that the disposable mechanical tidal cell culture device further comprises:
the tank body is provided with a first connecting piece;
the liquid storage container is a liquid storage bag, the liquid storage bag is made of flexible materials, and the liquid storage bag is provided with a second connecting piece;
the liquid storage bag is arranged in the tank body, and the first connecting piece is connected with the second connecting piece;
the tray is arranged in the tank body.
2. The disposable mechanical tidal cell culture device of claim 1, wherein:
when the liquid storage bag is in a liquid storage state, the liquid storage part of the liquid storage bag is abutted against the inner wall surface of the tank body, and/or the tray supports the liquid storage bag.
3. The disposable mechanical tidal cell culture device of claim 1 or 2, wherein:
an opening is formed in the top of the tank body;
the tank body further comprises a top cover, and the top cover is detachably arranged on the opening.
4. The disposable mechanical tidal cell culture device of claim 3, wherein:
the first connecting piece is arranged on the top cover;
the first connecting piece is detachably connected with the second connecting piece;
the liquid storage bag is hung on the top cover.
5. The disposable mechanical tidal cell culture device of claim 1 or 2, wherein:
the lifting device further comprises a driving unit, and the driving unit drives the tray;
the drive unit is arranged outside the tank body, and the tray is arranged inside the tank body.
6. The disposable mechanical tidal cell culture device of claim 1 or 2, wherein:
the liquid storage bag is also provided with a water outlet which is arranged at the bottom of the liquid storage bag.
7. The disposable mechanical tidal cell culture device of claim 6, wherein:
the bottom of the liquid storage bag comprises a first cambered surface protruding downwards, and the water outlet is formed in the lowest point of the first cambered surface.
8. The disposable mechanical tidal cell culture device of claim 7, wherein:
the tray is provided with a second cambered surface which is sunken downwards, and the second cambered surface is matched with the first cambered surface.
9. The disposable mechanically tidal cell culture device of claim 1 or 2, further comprising:
a carrier carrying a cell carrier; the liquid inlet pipe is communicated with the inside and the outside of the liquid storage bag;
the feed liquor pipe with the carrier is connected, the feed liquor pipe is hung the carrier, the mouth of pipe of feed liquor pipe avoids the carrier.
10. The disposable mechanical tidal cell culture device of claim 9, wherein:
the carrier is of a net structure or a porous structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221733867.4U CN217781174U (en) | 2022-07-06 | 2022-07-06 | Disposable mechanical tidal cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221733867.4U CN217781174U (en) | 2022-07-06 | 2022-07-06 | Disposable mechanical tidal cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217781174U true CN217781174U (en) | 2022-11-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221733867.4U Active CN217781174U (en) | 2022-07-06 | 2022-07-06 | Disposable mechanical tidal cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217781174U (en) |
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2022
- 2022-07-06 CN CN202221733867.4U patent/CN217781174U/en active Active
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