CN210900802U - Tissue disinfection device special for tissue culture - Google Patents

Tissue disinfection device special for tissue culture Download PDF

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Publication number
CN210900802U
CN210900802U CN201921786958.2U CN201921786958U CN210900802U CN 210900802 U CN210900802 U CN 210900802U CN 201921786958 U CN201921786958 U CN 201921786958U CN 210900802 U CN210900802 U CN 210900802U
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tissue
disinfection
tissue culture
net bag
device special
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巫桂芬
陆启皇
陆杰铭
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Abstract

The utility model relates to a tissue disinfection device special for tissue culture, which is provided with an ultrasonic generator, can further remove dust and bacteria at hidden positions in tissues, can improve the disinfection effect, and has simple operation and high working efficiency; the upper cover is arranged, so that the harm of volatile toxic substances such as mercury bichloride and the like to human bodies can be prevented; the net bag is movably connected to the upper cover, so that the tissue for plant tissue culture can be conveniently taken and placed, the tissue does not need to be taken out and placed additionally, the operation steps are reduced, and the pollution risk is also reduced; after the disinfection is finished, the supporting rod is adjusted to move upwards, so that the net bag can be moved to the position above the disinfectant, the tissues in the net bag can be effectively prevented from being damaged by the disinfectant, meanwhile, the disinfectant in the net bag drops into the disinfection tank, and the disinfectant is prevented from dropping in the process of transferring the tissues in the disinfection process, so that the harm to a human body is avoided; and a liquid outlet is arranged, so that disinfectant waste liquid can be conveniently collected.

Description

Tissue disinfection device special for tissue culture
Technical Field
The utility model relates to a tissue culture disinfection apparatus technical field of plant especially relates to a dedicated tissue degassing unit is banked up with earth to group.
Background
The tissue culture of plants is a new asexual propagation technology developed in recent decades based on the theory that plant cells have totipotency. Tissue culture of plants, also called in vitro culture in a broad sense, refers to a technique of separating desired tissues, organs, cells or protoplasts, etc. from plant bodies, inoculating them on a culture medium containing various nutrients and plant hormones under aseptic conditions by aseptic manipulation to obtain regenerated whole plants or producing other products having economic value. The narrow definition refers to the regeneration plant obtained by culturing each part of plant tissue for tissue culture, such as cambium, parenchyma, mesophyll tissue, endosperm, and the like, and also refers to the culture of callus generated from each organ in the culture process, and the callus is redifferentiated to form the regeneration plant. The most important step in tissue culture of plants is the cleaning and disinfection of the explant.
At present, a disinfectant is usually added into containers such as beakers and the like to clean explants, and the cleaning method is not only insufficient in cleaning force, but also easy to splash the disinfectant to cause unnecessary waste.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model aims to provide a tissue disinfection device special for tissue culture with simple structure and strong practicability.
In order to achieve the above purpose, the technical scheme of the utility model is that: a tissue disinfection device special for tissue culture comprises a disinfection tank; the upper part of the disinfection tank is provided with an opening, and an upper cover is arranged on the upper part of the disinfection tank;
the upper surface of the upper cover is provided with a supporting rod for driving the upper cover to lift up and down, and the bottom surface of the upper cover is provided with a ring adjusting line;
a hanging ring is arranged on the loop adjusting line, a net bag is detachably hung below the hanging ring, and the net bag is arranged in the sterilizing tank;
an ultrasonic generator is arranged below or outside the disinfection tank, and an output port of the ultrasonic generator corresponds to the net bag;
the bottom of the disinfection tank is provided with a liquid outlet, and a valve is arranged on the liquid outlet.
Furthermore, the hanging ring is movably connected to the loop adjusting line.
Furthermore, a hook is arranged on the net bag.
Furthermore, the supporting rod is a telescopic rod.
Furthermore, a compression spring is arranged below the supporting rod, one end of the compression spring is connected with a gear, and the gear drives the compression spring to ascend and descend through the cooperation of a lead screw, so that the supporting rod is controlled to ascend and descend.
Furthermore, the ultrasonic generator is provided with an ultrasonic frequency modulation knob in a matching manner, and the frequency modulation range of the ultrasonic frequency modulation knob is 20kHz-50 kHz.
The ultrasonic generator is characterized by further comprising a base, wherein timing equipment and a power switch are arranged on the base, the power switch is provided with two gears, one gear controls a power supply of the timing equipment, and the other gear controls the power supply of the timing equipment and the power supply of the ultrasonic generator simultaneously.
Further, the disinfection tank body is made of glass materials, and scale marks are arranged on the disinfection tank body.
Further, the net bag is made of stainless steel materials.
The utility model adopts the above technical scheme's beneficial effect is: the utility model relates to a tissue disinfection device special for tissue culture, which is provided with an ultrasonic generator, can further remove dust and bacteria at hidden positions in tissues, can improve the disinfection effect, and has simple operation and high working efficiency; the upper cover is arranged, so that the harm of volatile toxic substances such as mercury bichloride and the like to human bodies can be prevented; the net bag is movably connected to the upper cover, so that the tissue for plant tissue culture can be conveniently taken and placed, the tissue does not need to be taken out and placed additionally, the operation steps are reduced, and the pollution risk is also reduced; after the disinfection is finished, the supporting rod is adjusted to move upwards, so that the net bag can be moved to the position above the disinfectant, the tissues in the net bag can be effectively prevented from being damaged by the disinfectant, meanwhile, the disinfectant in the net bag drops into the disinfection tank, and the disinfectant is prevented from dropping in the process of transferring the tissues in the disinfection process, so that the harm to a human body is avoided; and a liquid outlet is arranged, so that disinfectant waste liquid can be conveniently collected.
The utility model relates to a tissue disinfection device special for tissue culture, the hanging rings are movably connected on the loop adjusting line, the distance between the hanging rings can be adjusted according to the diameter of the net bag, and the tissue disinfection device is suitable for net bags with different diameters; the supporting rod is a telescopic rod, the lifting of the upper cover can be manually adjusted, and the practicability is high; the frequency modulation range of the ultrasonic frequency modulation knob is 20kHz-50kHz, the penetrating power under the frequency is strong, plant tissues with complicated surface shapes or blind holes are favorably cleaned, the cavitation noise is small, but the cavitation intensity is low, and the ultrasonic frequency modulation knob is suitable for the condition that the bonding force between the cleaning dirt and the surface of the cleaned tissues is weak; the disinfection tank is provided with scale marks, the volume of the added cleaning or disinfection solution can be estimated according to the scale marks, and the phenomenon that the excessive cleaning or disinfection solution overflows the outside of the disinfection tank to cause secondary pollution and injury is prevented; the sterilizing tank is made of glass materials, so that the visibility is high, and the condition in the sterilizing tank can be observed conveniently in real time; the net bag is made of stainless steel materials, and the service life is long.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
The present invention will be further described with reference to the following drawings and examples, which should not be construed as limiting the invention in any way.
As shown in FIG. 1, a tissue sterilizing apparatus for tissue culture comprises a sterilizing tank 1; the upper part of the disinfection tank 1 is provided with an opening, and the upper cover 3 is arranged on the disinfection tank, so that the harm of volatile toxic substances such as mercury bichloride to human bodies can be prevented;
the upper cover 3 is provided with the supporting rod 4 for driving the upper cover to lift up and down, the bottom surface of the upper cover is provided with the loop adjusting line 7, the supporting rod 4 is adjusted to move upwards after disinfection is finished, the net bag 9 can be moved to the position above disinfectant, the tissue in the net bag 9 can be effectively prevented from being damaged by the disinfectant, meanwhile, the disinfectant in the net bag 9 is dropped into the disinfection tank 1, and the disinfectant is prevented from dropping in the process of transferring the tissue in the disinfection process, so that the injury to a human body is caused;
the ring adjusting line 7 is provided with a hanging ring 8, a net bag 9 is detachably hung below the hanging ring 8, the net bag 9 is arranged in the sterilizing tank 1, the net bag 9 is movably connected to the upper cover 3, the tissue for plant tissue culture is conveniently taken and placed, the tissue does not need to be taken out and placed additionally, the operation steps are reduced, and the pollution risk is also reduced;
an ultrasonic generator 10 is arranged below or outside the disinfection tank 1, and an output port of the ultrasonic generator 10 corresponds to the net bag 9, so that dust and bacteria at a hidden part in a tissue can be further removed, the disinfection effect can be improved, the operation is simple, and the working efficiency is high;
the bottom of disinfection jar 1 is equipped with leakage fluid dram 12, be provided with the valve on leakage fluid dram 12, be convenient for collect antiseptic solution waste liquid.
Preferably, this embodiment link 8 swing joint in adjust the ring line 7 on, can adjust the distance between the link 8 according to string bag 9 diameter size, be applicable to the string bag 9 of different diameters.
Preferably, the string bag 9 of the present embodiment is provided with a hook, so that the string bag 9 can be conveniently hung on the hanging ring 8.
Preferably, this embodiment bracing piece 4 is the telescopic link, but the lift of manual regulation upper cover 3, and the practicality is strong.
Preferably, the ultrasonic wave generator 10 of the present embodiment is provided with the ultrasonic frequency modulation knob 11 in a matching manner, the frequency modulation range of the ultrasonic frequency modulation knob 11 is 20kHz to 50kHz, the penetrating power under the frequency is strong, plant tissues with complicated surface shapes or blind holes are preferably cleaned, the cavitation noise is small, but the cavitation intensity is low, and the ultrasonic frequency modulation knob is suitable for the condition that the bonding force between the cleaning dirt and the surface of the cleaned tissue is weak.
Preferably, the present embodiment further comprises a base 2, the base 2 is provided with a timing device 13 and a power switch 14, the power switch 14 is provided with two gears, wherein one gear controls the power supply of the timing device 13, and the other gear controls the power supply of the timing device 13 and the power supply of the ultrasonic generator 10 simultaneously.
Preferably, the tank body 1 of the disinfection tank of the embodiment is made of a glass material, and the disinfection tank body is provided with scale marks 15, so that the volume of cleaning or disinfection solution added can be estimated according to the scale marks 15, and the phenomenon that excessive cleaning or disinfection solution overflows the outside of the disinfection tank 1 to cause secondary pollution and injury is prevented; the disinfection tank 1 is made of glass materials, has high visibility, and is convenient for observing the conditions in the disinfection tank 1 in real time.
Preferably, the net bag 9 of the embodiment is made of stainless steel materials, and has long service life.
As another embodiment of this embodiment, the below of bracing piece 4 is provided with compression spring 5, compression spring 5's one end is connected with gear 6, gear 6 passes through the screw cooperation and drives compression spring 5 oscilaltion to control bracing piece 4 oscilaltion has avoided manual operation, and the practicality is strong.
The utility model relates to a use method of a tissue disinfection device special for tissue culture, which comprises the following steps: 1) closing a valve on the liquid outlet 12, and adding disinfectant into the disinfection tank 1; 2) placing the tissue to be disinfected into the net bag 9, placing the net bag 9 into the disinfection tank 1 and hanging the net bag on the hanging ring 8, and closing the upper cover 3; 3) turning on the ultrasonic generator 10; 4) after the disinfection is finished, the ultrasonic generator 10 is closed, and the net bag 9 is lifted to be above the liquid level of the disinfectant; 5) when no liquid in the net bag 9 continuously drops into the disinfection tank 1, the valve on the liquid outlet 12 is opened, and the liquid collecting bottle is used for collecting the disinfectant waste liquid.
The foregoing shows and describes the general principles and features of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (9)

1. A tissue disinfection device special for tissue culture is characterized by comprising a disinfection tank; the upper part of the disinfection tank is provided with an opening, and an upper cover is arranged on the upper part of the disinfection tank;
the upper surface of the upper cover is provided with a supporting rod for driving the upper cover to lift up and down, and the bottom surface of the upper cover is provided with a ring adjusting line;
a hanging ring is arranged on the loop adjusting line, a net bag is detachably hung below the hanging ring, and the net bag is arranged in the sterilizing tank;
an ultrasonic generator is arranged below or outside the disinfection tank, and an output port of the ultrasonic generator corresponds to the net bag;
the bottom of the disinfection tank is provided with a liquid outlet, and a valve is arranged on the liquid outlet.
2. The tissue disinfection device special for tissue culture according to claim 1, wherein the hanging ring is movably connected to the loop adjusting line.
3. The tissue disinfection device special for tissue culture according to claim 1, wherein the string bag is provided with a hook.
4. The tissue disinfection device special for tissue culture according to claim 1, wherein the support rod is a telescopic rod.
5. The tissue disinfection device special for tissue culture according to claim 1, wherein a compression spring is arranged below the support rod, one end of the compression spring is connected with a gear, and the gear drives the compression spring to move up and down through the cooperation of a lead screw, so that the support rod is controlled to move up and down.
6. The tissue disinfection device special for tissue culture as claimed in claim 1, wherein the ultrasonic generator is provided with an ultrasonic frequency modulation knob in a matching way, and the frequency modulation range of the ultrasonic frequency modulation knob is 20kHz-50 kHz.
7. The tissue disinfection device special for tissue culture according to claim 1, further comprising a base, wherein the base is provided with a timing device and a power switch, the power switch is provided with two gears, one gear controls the power of the timing device, and the other gear controls the power of the timing device and the power of the ultrasonic generator.
8. The tissue disinfection device special for tissue culture according to claim 1, wherein the disinfection tank body is made of glass material, and the disinfection tank body is provided with scale marks.
9. The tissue sterilizer special for tissue culture according to claim 1, wherein the net bag is made of stainless steel material.
CN201921786958.2U 2019-10-23 2019-10-23 Tissue disinfection device special for tissue culture Active CN210900802U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921786958.2U CN210900802U (en) 2019-10-23 2019-10-23 Tissue disinfection device special for tissue culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921786958.2U CN210900802U (en) 2019-10-23 2019-10-23 Tissue disinfection device special for tissue culture

Publications (1)

Publication Number Publication Date
CN210900802U true CN210900802U (en) 2020-07-03

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CN201921786958.2U Active CN210900802U (en) 2019-10-23 2019-10-23 Tissue disinfection device special for tissue culture

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113287523A (en) * 2021-06-08 2021-08-24 陈嘉裔 Intermittent fast-immersion succulent plant propagation and disinfection device and implementation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113287523A (en) * 2021-06-08 2021-08-24 陈嘉裔 Intermittent fast-immersion succulent plant propagation and disinfection device and implementation method thereof

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