CN217578922U - Device for in vitro culturing fertilized egg and blastocyst - Google Patents
Device for in vitro culturing fertilized egg and blastocyst Download PDFInfo
- Publication number
- CN217578922U CN217578922U CN202220966841.8U CN202220966841U CN217578922U CN 217578922 U CN217578922 U CN 217578922U CN 202220966841 U CN202220966841 U CN 202220966841U CN 217578922 U CN217578922 U CN 217578922U
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- Prior art keywords
- fixedly connected
- wall
- incubator
- baffle
- blastocyst
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- 210000002459 blastocyst Anatomy 0.000 title claims abstract description 19
- 238000000338 in vitro Methods 0.000 title claims description 15
- 238000012258 culturing Methods 0.000 title claims description 10
- 210000004907 gland Anatomy 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims abstract description 16
- 238000007789 sealing Methods 0.000 claims description 13
- 230000005611 electricity Effects 0.000 claims description 11
- 238000010030 laminating Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 11
- 238000003860 storage Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 235000013601 eggs Nutrition 0.000 description 20
- 210000002257 embryonic structure Anatomy 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000029803 blastocyst development Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a fertilized egg cultivates technical field, and discloses an external device of cultivateing fertilized egg and blastocyst stage embryo, including the incubator, the top fixedly connected with end cover of incubator, the center fixedly connected with support column of department of incubator, the bottom fixedly connected with power of support column, the inside set screw hole of end cover, the inner wall threaded connection of screw hole has the gland, the bottom fixedly connected with body of gland, the bottom of body extends to the bottom inner wall of incubator, the inside fixedly connected with constant temperature heating pipe of body, the bottom of constant temperature heating pipe extends to the bottom and the fixedly connected with connection inserted block of body. The utility model discloses can regulate and control the internal constant temperature of geminate transistors, improve the storage quality of embryonated egg, can play inseparable closed effect to culture apparatus when taking out the body simultaneously, guarantee to cultivate the environment.
Description
Technical Field
The utility model relates to a fertilized egg cultivates technical field, especially relates to an external device of cultivateing fertilized egg and blastocyst stage embryo.
Background
With the development of modern reproduction biotechnology and the gradual improvement of embryo in vitro production technology, the sheep embryo in vitro production technology has become an important way for accelerating the speed of variety breeding and excellent population propagation, but in the culture process of in vitro embryos obtained by in vitro fertilization or cloning, a development blocking phenomenon generally exists, namely, the embryos stop developing after a certain stage and degenerate, so that the blastocyst development rate of the in vitro embryos is far lower than that of in vivo embryos.
Through retrieval, the Chinese published patent No. CN110747160A discloses a method for culturing sheep fertilized eggs with high survival rate for in vitro culture of embryo bodies, which comprises the following steps: the method comprises the following steps of oocyte culture, semen treatment, in-vitro fertilization and fertilized ovum culture, wherein the fertilized ovum culture needs to be carried out in an incubator, and the incubator comprises an incubator body, a plurality of culture dishes arranged in the incubator body, and a lifting device used for independently lifting the culture dishes;
among the above-mentioned patent scheme, can not carry out constant temperature regulation and control to the body that holds the embryonated egg, can influence the storage quality of its inside embryo, and can not seal culture apparatus when taking out the body, can influence the culture environment. Therefore, the utility model designs a device for culturing fertilized eggs and embryos in the blastocyst stage in vitro.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving and can not carry out constant temperature regulation and control to the body that holds the embryonated egg among the prior art, can influence the storage quality of its inside embryo, and can not seal culture apparatus when taking out the body, can influence the problem of cultivateing the environment, and the device of an external culture embryonated egg and blastocyst embryonic stage embryo of proposition.
In order to realize the purpose, the utility model adopts the following technical scheme:
the utility model provides an external device of cultivateing embryonated egg and blastocyst stage embryo, includes the incubator, the top fixedly connected with end cover of incubator, the fixedly connected with support column is located at the center of incubator, the bottom fixedly connected with power of support column, the inside threaded hole that sets up of end cover, the inner wall threaded connection of screw hole has the gland, the bottom fixedly connected with body of gland, the bottom of body extends to the bottom inner wall of incubator, the inside fixedly connected with constant temperature heating pipe of body, the bottom of constant temperature heating pipe extends to the bottom and the fixedly connected with of body and connects the inserted block, the bottom inner wall of incubator is seted up flutedly, the inner wall fixedly connected with electricity connection plate of recess, the bottom fixedly connected with connecting wire of electricity connection plate, the connecting wire deviates from the one end of electricity connection plate and is connected with the power electricity.
Preferably, the inner wall of the gland above the pipe body is provided with a through hole, the inner wall of the top end of the through hole is provided with a sealing plug in an inserting manner, the side wall of the bottom end of the gland is fixedly connected with a sealing ring, and the inner wall of the pipe body is fixedly connected with a heat insulation pad.
Preferably, the inner wall that is located the left and right both sides of recess of incubator has been seted up respectively and has been accomodate the groove, two the inner wall in accomodating the groove is fixedly connected with spring, two respectively the spring is close to the one end fixedly connected with slide respectively of connecting the inserted block, two the one end that the slide deviates from the spring extends to the outside of accomodating the groove and compresses tightly the setting simultaneously on the lateral wall of connecting the inserted block.
Preferably, the top side walls of the two sliding plates are respectively provided with an inclined surface, and the bottom side wall of the connecting insertion block is provided with a chamfer angle matched with the sliding plate.
Preferably, the top inner wall fixedly connected with of incubator keeps off the ring, the body slides and sets up the inner wall at keeping off the ring, the bottom inner wall that keeps off the ring encircles and has seted up a plurality of draw-in grooves, the inner wall of draw-in groove rotates and is connected with the pivot, cup joint simultaneously on the axle wall of pivot and be provided with torsional spring and baffle, the one end fixed connection of torsional spring is at the inner wall that keeps off the ring, the other end and the baffle fixed connection of torsional spring, the bottom laminating setting of baffle is on the lateral wall of body.
Preferably, the top end of the baffle is fixedly connected with a sealing gasket, and the baffle is in a fan shape.
Preferably, the inner wall fixedly connected with bracing piece of incubator, the bracing piece is close to fixedly connected with support frame on the lateral wall of body, the support frame deviates from one side laminating setting of bracing piece on the pipe wall of body.
Compared with the prior art, the utility model provides an in vitro device of cultivateing embryonated egg and blastocyst stage embryo possesses following beneficial effect:
1. this device of external culture embryonated egg and blastocyst embryo, the inside that extends to the recess through the inserted block is connected with the electricity connection piece electricity, makes the constant temperature heating pipe circular telegram set up to can make the constant temperature heating pipe carry out the constant temperature heating, make the body around stable rise to the temperature that is fit for the embryonated egg and store, improve the storage quality of embryonated egg.
2. This device of external culture embryonated egg and blastocyst stage embryo rotates on the axle wall through the torsional drive baffle along the pivot, makes a plurality of baffles can align each other and paste tight setting to can seal the inner wall that keeps off the ring, can play inseparable protective effect to the inside cultivation environment of incubator.
The part that does not relate to among the device all is the same with prior art or can adopt prior art to realize, the utility model discloses can carry out constant temperature regulation and control to the internal portion of geminate transistors, improve the storage quality of embryonated egg, can play inseparable closed effect to culture apparatus when taking out the body simultaneously, guarantee to cultivate the environment.
Drawings
FIG. 1 is a schematic view showing the structure of an apparatus for in vitro culture of fertilized eggs and blastocyst;
FIG. 2 is an enlarged view of a portion A of FIG. 1;
FIG. 3 is an enlarged view of a portion B of FIG. 1;
FIG. 4 is a top view of the structure of the baffle of FIG. 3;
fig. 5 is a perspective view of the tube body in fig. 3.
In the figure: the device comprises an incubator 1, an end cover 2, a support column 3, a power supply 4, a gland 5, a pipe body 6, a constant-temperature heating pipe 7, a connection plug 8, an electric connection sheet 9, a connection lead 10, a sealing plug 11, a sealing ring 12, a spring 13, a sliding plate 14, a heat preservation pad 15, a baffle ring 16, a rotating shaft 17, a torsion spring 18, a baffle plate 19, a sealing pad 20, a support rod 21 and a support frame 22.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example one
Referring to fig. 1, fig. 2 and fig. 5, an in vitro device for culturing fertilized eggs and blastocyst embryos, including an incubator 1, a top fixedly connected with end cover 2 of the incubator 1, a center fixedly connected with support column 3 of the incubator 1, a bottom fixedly connected with power supply 4 of the support column 3, a threaded hole is provided in the end cover 2, an inner wall threaded connection of the threaded hole has a gland 5, a bottom fixedly connected with tube body 6 of the gland 5, a bottom end of the tube body 6 extends to an inner wall of the bottom of the incubator 1, an inside fixedly connected with constant temperature heating tube 7 of the tube body 6, a bottom end of the constant temperature heating tube 7 extends to the bottom end of the tube body 6 and is fixedly connected with a connection insert 8, a groove is provided in the inner wall of the bottom of the incubator 1, an inner wall fixedly connected with an electric connection sheet 9 of the groove, a bottom fixedly connected with a connection wire 10 of the electric connection sheet 9, one end of the connection wire 10 deviating from the electric connection sheet 9 is electrically connected with the power supply 4, a through hole is provided in the inner wall of the gland 5 above the tube body 6, a sealing plug 11 is inserted in the top inner wall of the through hole, a bottom end side wall of the gland 5 is fixedly connected with a sealing ring 12, and a heat preservation pad 15 is fixedly connected with the inner wall of the tube 6.
Place the embryonated egg in the inside of body 6, then insert sealing plug 11 tightly at the top inner wall that gland 5 is located through-hole department, thereby can extend body 6 to the inside of incubator 1 with the inner wall of gland 5 threaded connection in the screw hole, the connection inserted block 8 of body 6 bottom can extend to the inside and the electric connection piece 9 electricity of recess and be connected, thereby can make power 4 can transmit the electric energy to connecting inserted block 8 and constant temperature heating pipe 7 through wire 10 and electric connection piece 9, make constant temperature heating pipe 7 circular telegram set up, thereby can make constant temperature heating pipe 7 carry out the constant temperature heating, make the temperature that steadily risees to being fit for the embryonated egg to store around the body 6, thereby can improve the storage quality of embryonated egg, gland 5 and the inside leakproofness of incubator 1 can be guaranteed to sealing ring 12.
Example two
Referring to fig. 2, incubator 1 be located the recess left side, the groove has been seted up respectively to the inner wall of right both sides and has been accomodate the groove, two inner walls of accomodating the groove are fixedly connected with spring 13 respectively, two spring 13 are close to the one end of connecting inserted block 8 fixedly connected with slide 14 respectively, two slide 14 deviate from the one end of spring 13 and extend to the outside of accomodating the groove and compress tightly the setting simultaneously on the lateral wall of connecting inserted block 8, the inclined plane has been seted up respectively to the top lateral wall of two slides 14, the bottom lateral wall of connecting inserted block 8 seted up with slide 14 assorted bevel angle.
After body 6 shifted out to the outside of incubator 1, thereby spring 13 can make slide 14 compress tightly the setting each other with slide 14 ejecting outside to accomodating the groove, slide 14 adopts rubber to make, thereby can set up the top seal of recess, and can have certain insulating effect, prevent the condition of electric connection piece 9 made by the metal electric leakage, slide 14's top is provided with the inclined plane, and the bottom lateral wall of connecting inserted block 8 has seted up the chamfer angle, can not influence the inside of connecting inserted block 8 and pegging graft to the recess.
EXAMPLE III
Referring to fig. 3 and 4, the top inner wall fixedly connected with of incubator 1 keeps off ring 16, body 6 slides and sets up the inner wall that keeps off ring 16, the bottom inner wall that keeps off ring 16 encircles and has seted up a plurality of draw-in grooves, the inner wall of draw-in groove rotates and is connected with pivot 17, cup joint simultaneously on the axle wall of pivot 17 and be provided with torsional spring 18 and baffle 19, the one end fixed connection of torsional spring 18 is at the inner wall that keeps off ring 16, the other end and the 19 fixed connection of baffle 18, the bottom laminating of baffle 19 sets up on the lateral wall of body 6, the sealed pad 20 of top fixedly connected with of baffle 19, baffle 19 sets up to fan-shaped.
When body 6 shifted out behind the outside of incubator 1, the one end of torsional spring 18 can kick-back and make baffle 19 rotate on the axle wall of pivot 17, press close to the inner wall that sets up at the draw-in groove until the top of baffle 19, make a plurality of baffles 19 can align each other and paste tight setting, thereby can seal the inner wall that keeps off ring 16, prevent to take out behind incubator 1 outside at body 6, the inner wall of incubator 1 receives the pollution influence and cultivates the environment, can play inseparable protective effect to the cultivation environment of incubator 1 inside.
Example four
Referring to fig. 1, inner wall fixedly connected with bracing piece 21 of incubator 1, fixedly connected with support frame 22 on bracing piece 21 is close to the lateral wall of body 6, and support frame 22 deviates from one side laminating setting of bracing piece 21 on the pipe wall of body 6.
The above, only be the embodiment of the preferred of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, which are designed to be replaced or changed equally, all should be covered within the protection scope of the present invention.
Claims (7)
1. The utility model provides an in vitro device of cultivateing embryonated egg and blastocyst, includes incubator (1), its characterized in that: the utility model discloses a cultivation box, including incubator (1), top fixedly connected with end cover (2), the center department fixedly connected with support column (3) of incubator (1), the bottom fixedly connected with power (4) of support column (3), the inside threaded hole of end cover (2), the inner wall threaded connection of screw hole has gland (5), the bottom fixedly connected with body (6) of gland (5), the bottom inner wall of body (6) extends to incubator (1), the inside fixedly connected with constant temperature heating pipe (7) of body (6), the bottom of constant temperature heating pipe (7) extends to the bottom and the fixedly connected with of body (6) and connects inserted block (8), the bottom inner wall of incubator (1) is seted up flutedly, the inner wall fixedly connected with electricity connection piece (9) of recess, the bottom fixedly connected with connecting wire (10) of electricity connection piece (9), connecting wire (10) deviate from the one end and the power (4) electricity of electricity connection piece (9).
2. The apparatus for culturing fertilized egg and blastocyst according to claim 1, wherein: the inner wall that gland (5) are located body (6) top has seted up the through-hole, the grafting of the top inner wall of through-hole is provided with sealing plug (11), the bottom lateral wall fixedly connected with sealing ring (12) of gland (5), the inner wall fixedly connected with heat preservation pad (15) of body (6).
3. The apparatus for culturing fertilized egg and blastocyst according to claim 1, wherein: the inner wall that is located the left and right both sides of recess of incubator (1) has been seted up respectively and has been accomodate the groove, two the inner wall in accomodating the groove is fixedly connected with spring (13) respectively, two spring (13) are close to the one end difference fixedly connected with slide (14) of connecting inserted block (8), two the one end that slide (14) deviates from spring (13) extends to the outside of accomodating the groove and compresses tightly the setting simultaneously on the lateral wall of connecting inserted block (8).
4. The apparatus for culturing fertilized egg and blastocyst according to claim 3, wherein: inclined planes are respectively arranged on the side walls of the top ends of the two sliding plates (14), and chamfer angles matched with the sliding plates (14) are arranged on the side walls of the bottom ends of the connecting insertion blocks (8).
5. The device for in vitro culturing of the fertilized egg and the blastocyst embryo according to claim 1, wherein the inner wall of the top end of the incubator (1) is fixedly connected with a baffle ring (16), the tube body (6) is slidably arranged on the inner wall of the baffle ring (16), the inner wall of the bottom end of the baffle ring (16) is surrounded by a plurality of clamping grooves, the inner wall of each clamping groove is rotatably connected with a rotating shaft (17), the shaft wall of the rotating shaft (17) is simultaneously sleeved with a torsion spring (18) and a baffle (19), one end of the torsion spring (18) is fixedly connected to the inner wall of the baffle ring (16), the other end of the torsion spring (18) is fixedly connected with the baffle (19), and the bottom end of the baffle (19) is attached to the side wall of the tube body (6).
6. The apparatus for culturing fertilized egg and blastocyst according to claim 5, wherein: the top end of the baffle (19) is fixedly connected with a sealing gasket (20), and the baffle (19) is fan-shaped.
7. The apparatus for culturing fertilized egg and blastocyst according to claim 1, wherein: inner wall fixedly connected with bracing piece (21) of incubator (1), fixedly connected with support frame (22) on bracing piece (21) are close to the lateral wall of body (6), one side laminating that support frame (22) deviates from bracing piece (21) sets up on the pipe wall of body (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220966841.8U CN217578922U (en) | 2022-04-25 | 2022-04-25 | Device for in vitro culturing fertilized egg and blastocyst |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220966841.8U CN217578922U (en) | 2022-04-25 | 2022-04-25 | Device for in vitro culturing fertilized egg and blastocyst |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217578922U true CN217578922U (en) | 2022-10-14 |
Family
ID=83543083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220966841.8U Expired - Fee Related CN217578922U (en) | 2022-04-25 | 2022-04-25 | Device for in vitro culturing fertilized egg and blastocyst |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217578922U (en) |
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2022
- 2022-04-25 CN CN202220966841.8U patent/CN217578922U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221014 |
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| CF01 | Termination of patent right due to non-payment of annual fee |