CN217578853U - Magnetic frame of movable magnet - Google Patents

Abstract

The application relates to a magnetic frame of a movable magnet, which can move in a guide groove through the cooperation of the guide groove, an elastic part, a clamping plate and the guide rod, so that the clamping plate can clamp centrifuge tubes with different calibers, namely, a centrifuge tube clamping groove can clamp centrifuge tubes with different calibers, thereby improving the practicability of the magnetic frame; through set up the spout on perpendicular landing leg, set up mobilizable magnet piece in the spout, so, adsorb the magnetic bead that the magnet piece can not adsorb by removing the magnet piece, compare with prior art, reduced the loss of nucleic acid, and then improve the DNA content that the elution obtained, practice thrift the experimental time.

Description

Magnetic frame of movable magnet

Technical Field

The utility model relates to a biological gene detects and experimental field, especially involves a magnetic frame of portable magnet.

Background

The magnetic frame is a necessary device for extracting nucleic acid by a nano magnetic bead method. The nanometer magnetic bead adsorbs the target substance, concentrates the adsorption of nanometer magnetic bead through the magnetic frame and is close to the position of magnet in the centrifuging tube, can realize quick solid-liquid separation, gets rid of the purpose of impurity, makes things convenient for target substance to retrieve the collection. Magnetic racks are also currently used in related proteomics experiments using nanobeads.

The magnetic bead method is to lyse a sample with a lysis solution, and after the sample is lysed, nucleic acid molecules are specifically adsorbed to the surface of magnetic particles, while impurities such as proteins are not adsorbed and remain in the solution. After reacting for a certain time, separating the magnetic particles from the liquid under the action of a magnetic field, recovering the particles (namely the magnetic bead-DNA conjugate), washing with a washing solution to remove impurities which are not specifically adsorbed on the magnetic beads, and then eluting to obtain pure DNA.

At present, the magnetic force frame used in relevant experiments at home and abroad is a traditional I-shaped structure, the upper end of the magnetic force frame is a centrifugal tube clamping groove, the lower end of the magnetic force frame is a base with a counterweight, and magnets embedded in vertical supporting legs can adsorb magnetic beads to the side wall or the bottom of a centrifugal tube. The popularization and the application of the traditional structure bring convenience to the proteomics experiment operation. The structure has the following defects that reagents with different volumes can be added in the experimental process, and when a small amount of reagents are added due to the fixed magnet position, magnetic beads in the solution cannot be completely adsorbed on a centrifugal tube, so that the loss of nucleic acid is caused, and the content of DNA obtained by final elution is influenced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a movable magnet's magnetic force frame, it can retrieve the magnetic bead completely, reduces the loss, and the practicality is higher.

In order to achieve the above purpose, the utility model provides a following technical scheme: the utility model provides a portable magnet's magnetic force frame, the magnetic force frame includes the base and is used for installing the centrifuge tube cardboard perpendicular landing leg on the base, be provided with the spout on the perpendicular landing leg, the card has the magnet piece in the spout, the magnet piece can remove in the spout, be provided with between magnet piece and the spout and be used for with the magnet piece location is in location structure in the spout, be provided with a plurality of centrifuging tube draw-in grooves on the centrifuge tube cardboard, every be provided with at least one guide way on the inner wall of centrifuging tube draw-in groove, installation elastic component in the guide way, install the card in the centrifuging tube draw-in groove and hold the board, the card is held and is provided with the guide bar on the board, elastic component one end is connected on the guide bar, and the other end is connected in the guide way, the guide bar can remove in the guide way.

Furthermore, the spout is protruding type spout, be provided with on the magnet piece with protruding type spout complex T type fixture block, location structure includes certainly a plurality of locating holes of the inside wall of protruding type spout inwards sunken formation, a plurality of the locating hole extends along the length direction of protruding type spout, be provided with on the trailing flank of T type fixture block with one of them locating hole complex location bump.

Further, the contact surface that the centrifuging tube was held to the card is held sets up to the arcwall face in order to laminate the centrifuging tube.

Furthermore, be provided with the location fixed orifices on the base, the magnet piece has been inlayed to location fixed orifices bottom.

Furthermore, the centrifugal tube clamping groove and the positioning fixing hole are correspondingly arranged.

Further, the vertical leg is vertically connected with the base.

Further, the vertical leg is vertically connected to the centrifuge tube clamp plate.

Further, a balancing weight is arranged on the base.

The beneficial effects of the utility model reside in that: according to the centrifugal frame, the guide groove, the elastic piece, the clamping plate and the guide rod are matched, so that the guide rod can move in the guide groove, the clamping plate can clamp centrifugal tubes with different pipe diameters, namely, a centrifugal tube clamping groove can clamp centrifugal tubes with different pipe diameters, and therefore the practicability of the magnetic frame is improved; through set up the spout on perpendicular landing leg, set up mobilizable magnet piece in the spout, so, adsorb the magnetic bead that the magnet piece can not adsorb by removing the magnet piece, compare with prior art, reduced the loss of nucleic acid, and then improve the DNA content that the elution obtained, practice thrift the experimental time.

The above description is only an outline of the technical solution of the present invention, and in order to make the technical means of the present invention more clearly understood and to be implemented in accordance with the content of the specification, the following detailed description will be given of preferred embodiments of the present invention in conjunction with the accompanying drawings.

Drawings

Fig. 1 is a structural view of a magnetic frame of a movable magnet according to an embodiment of the present application.

FIG. 2 is an enlarged cross-sectional view of the neck of the centrifuge tube of FIG. 1.

Fig. 3 is an enlarged sectional view of the runner of the magnetic frame of fig. 1.

Fig. 4a is a front view of a magnet block according to an embodiment of the present application.

Fig. 4b is a side view of the magnet block shown in fig. 4 a.

Detailed Description

The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

Referring to fig. 1, the magnetic force frame of a portable magnet that this application a preferred embodiment shows includes base 2 and is used for installing centrifuge tube cardboard 1 perpendicular landing leg 4 on the base 2, be provided with spout 3 on perpendicular landing leg 4, the card has magnet piece 8 in the spout 3, magnet piece 8 can remove in the spout 3, be provided with between magnet piece 8 and the spout 3 for with magnet piece 8 is fixed a position location structure in the spout, be provided with a plurality of centrifuging tube draw-in grooves 5 on the centrifuge tube cardboard 1, every be provided with at least one guide way 10 on centrifuge tube draw-in groove 5's the inner wall, installation elastic component 12 in the guide way 10, install card clamping plate 11 in the centrifuging tube draw-in groove 5, the card is provided with guide bar 10 on clamping plate 11, elastic component 12 one end is connected on guide bar 10, and the other end is connected in guide way 10, guide bar 10 can remove in guide way 10.

In this embodiment, the vertical leg 4 is attached to the center of the centrifuge tube card 1 and base 2 to divide the magnetic rack into front and back sections. 1 centrifuging tube draw-in groove 5, 1 spout 3 and 1 location fixed orifices 6 are a set of setting that corresponds, the symmetries is provided with 6 sets of centrifuging tube draw-in grooves 5 around magnetic frame two parts, spout 3 and location fixed orifices 6, the spring is chooseed for use to elastic component 12, spring 12 is connected in the centre of guide bar 10, 10 one end of guide bar is connected and is held board 11, the other end of guide bar 10 can hold board 11 removal in order to drive the card at the inside removal of spring 12, thereby can hold the centrifuging tube that the pipe diameter is at 10.5mm (1.5 ml's centrifuging tube) to 17mm (15 ml's centrifuging tube).

Referring to fig. 3 to 4b, in this embodiment, the sliding groove 3 is a convex sliding groove, the magnet block 8 is provided with a T-shaped fixture block 81 engaged with the convex sliding groove 3, the positioning structure includes a plurality of positioning holes 31 formed by inward recessing from an inner side wall of the convex sliding groove 3, the plurality of positioning holes 31 extend along a length direction of the convex sliding groove 3, and a positioning protrusion 82 engaged with one of the positioning holes 31 is provided on a rear side surface of the T-shaped fixture block 81. The size of the T-shaped fixture block 81 is smaller than that of the convex chute 3, and the T-shaped fixture block can be pulled out to separate the positioning salient point 82 from the positioning hole 31, so that the T-shaped fixture block is moved to the next positioning hole 31 in the chute 3, and the positioning salient point 82 of the T-shaped fixture block is inserted into the positioning hole 31 to change the positioning of the magnet block.

In order to fix the centrifuging tube better, in this embodiment, the contact surface that the centrifuging tube was held to card 11 card of holding sets up to the arcwall face in order to laminate the centrifuging tube, is provided with location fixed orifices 6 on the base 2, and 6 bottoms in location fixed orifices are inlayed and are had magnet piece 7, and centrifuging tube draw-in groove 5 corresponds the setting with location fixed orifices 6.

Perpendicular landing leg 4 is connected with base 2 is perpendicular, and perpendicular landing leg 4 is connected with centrifuging tube cardboard 1 is perpendicular, is provided with the balancing weight on the base 2 not shown in the figure in order to increase the stability of magnetic frame.

The working principle of the magnetic frame is as follows: insert until the centrifuging tube bottom supports and holds in corresponding location fixed orifices by the centrifuging tube draw-in groove that will be equipped with the reagent, align with the magnet piece that location fixed orifices bottom set up, the magnet piece adsorbs the magnetic bead in the centrifuging tube, adsorb with the magnetic bead that the magnet piece that can not adsorb that sets up bottom the location fixed orifices through adjusting the position of magnet piece in protruding type spout, thereby reduce the nucleic acid loss, improve the DNA content that the elution obtained, thereby practice thrift the test time.

In conclusion: according to the centrifugal frame, the guide groove, the elastic piece, the clamping plate and the guide rod are matched, so that the guide rod can move in the guide groove, the clamping plate can clamp centrifugal tubes with different pipe diameters, namely, a centrifugal tube clamping groove can clamp centrifugal tubes with different pipe diameters, and therefore the practicability of the magnetic frame is improved; through set up the spout on perpendicular landing leg, set up mobilizable magnet piece in the spout, so, adsorb the magnetic bead that the magnet piece can not adsorb by removing the magnet piece, compare with prior art, reduced the loss of nucleic acid, and then improve the DNA content that the elution obtained, practice thrift the experimental time.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent several embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, variations and modifications can be made without departing from the spirit of the invention, and these are all within the scope of the invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims (8)

1. The utility model provides a portable magnet's magnetic frame, a serial communication port, the magnetic frame includes base (2) and is used for installing centrifuge tube cardboard (1) perpendicular landing leg (4) on base (2), be provided with spout (3) on perpendicular landing leg (4), card-held magnet piece (8) in spout (3), magnet piece (8) can remove in spout (3), be provided with between magnet piece (8) and spout (3) and be used for with magnet piece (8) location is in location structure in the spout, be provided with on centrifuge tube cardboard (1) and have a plurality of centrifuging tube draw-in grooves (5), every be provided with on centrifuge tube draw-in groove (5) the inner wall on centrifuging tube draw-in groove (5) at least one guide way (9), installation elastic component (12) in guide way (9), install in centrifuging tube draw-in groove (5) and hold flitch (11), be provided with guide bar (10) on centrifuging tube draw-in groove (11), elastic component (12) one end is connected on guide bar (10), and the other end is connected in guide way (9), guide bar (10) can block and remove in guide way (9).

2. The magnetic frame of a movable magnet according to claim 1, wherein the slide groove (3) is a convex slide groove, the magnet block (8) is provided with a T-shaped block (81) engaged with the convex slide groove (3), the positioning structure comprises a plurality of positioning holes (31) formed by inward recessing from an inner side wall of the convex slide groove (3), the plurality of positioning holes extend along a length direction of the convex slide groove (3), and a positioning protrusion (82) engaged with one of the positioning holes (31) is provided on a rear side surface of the T-shaped block (81).

3. The magnetic rack of movable magnet according to claim 1, wherein the contact surface of the chucking plate (11) for chucking the centrifugal tube is provided as an arc-shaped surface to fit the centrifugal tube.

4. The magnetic frame of a movable magnet according to claim 1, wherein the base (2) is provided with a positioning and fixing hole (6), and a magnet piece (7) is embedded in the bottom of the positioning and fixing hole (6).

5. The magnetic rack of movable magnet according to claim 4, characterized in that the centrifugal tube slot (5) is disposed corresponding to the positioning fixing hole (6).

6. The magnetic frame of a movable magnet according to claim 1, characterized in that said vertical leg (4) is connected vertically to said base (2).

7. The magnetic rack of movable magnets of claim 6, characterized in that the vertical leg (4) and the centrifuge tube card (1) are vertically connected.

8. The magnetic frame of a movable magnet according to claim 7, characterized in that a weight is provided on said base (2).

Images