CN217516649U - Automatic capping and capping device for PCR amplification plate - Google Patents

Automatic capping and capping device for PCR amplification plate Download PDF

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Publication number
CN217516649U
CN217516649U CN202221011552.9U CN202221011552U CN217516649U CN 217516649 U CN217516649 U CN 217516649U CN 202221011552 U CN202221011552 U CN 202221011552U CN 217516649 U CN217516649 U CN 217516649U
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CN
China
Prior art keywords
lifting
capping
plate
pin
gland
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CN202221011552.9U
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Chinese (zh)
Inventor
沈磊
刘雄杰
沈海东
陈任远
李源
王国强
刘中华
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Shanghai Shuoying Biotechnology Co ltd
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Jiangsu Bioperfectus Technologies Co ltd
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Priority to CN202221011552.9U priority Critical patent/CN217516649U/en
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Abstract

The utility model provides an automatic capping and capping device for a PCR amplification plate, which comprises a mounting frame, a first lifting component, a lifting frame, a second lifting component, a capping pin plate, a capping pin and a capping plate; the first lifting assembly is arranged on the mounting frame; the lifting frame is arranged on the first lifting assembly, and the first lifting assembly drives the lifting frame to lift; the second lifting assembly is arranged on the lifting frame, the binding pin plate is arranged on the second lifting assembly, the binding pin is arranged on the binding pin plate, the second lifting assembly drives the binding pin plate to lift, and the binding pin plate drives the binding pin to lift; the gland plate is arranged on the lifting frame, the gland pin penetrates through the gland plate, and the gland pin and the gland plate can slide relatively. The utility model discloses a gland lead screw motor with move back the cooperation of lid lead screw motor, combine to prick the alternately going up and down straggly of lid pin and gland board, realize pricking the automation of 96 hole silica gel covers and get, remove and the gland.

Description

Automatic capping and capping device for PCR amplification plate
Technical Field
The utility model relates to a check out test set technical field specifically, relates to an automatic capping device that adds of PCR amplification board.
Background
When nucleic acid detection is performed in a laboratory, reagents in a 96-well amplification plate are usually sealed by a membrane or manually pressing a 96-well silica gel cover 10 to seal the reagents in a 96-well amplification plate 11, which is time-consuming and labor-consuming, low in efficiency and possibly has the problem of no pressing.
The patent document with publication number CN106430047A discloses a capping system for PCR reaction tubes and a method for using the same, wherein the system realizes the up-and-down movement of a capping device through a ball screw, a driving wheel, a driven wheel, a connecting piece and a driving motor, because the PCR reaction tubes are placed on a reaction tube carrier, the tube caps of the PCR reaction tubes are placed in the capping device, and the capping device is located on the upper part of a bearing base, when the capping device moves downwards, the tube caps of the PCR reaction tubes can be stably capped on the PCR reaction tubes under the driving of the capping device, without manually and row-by-row compressing the tube caps of the PCR reaction tubes, the operation is convenient, and the capping is uniform. However, this patent document is different from the solution of the present application.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects in the prior art, the utility model aims at providing an automatic capping device for a PCR amplification plate.
According to the utility model, the automatic capping and capping device for the PCR amplification plate comprises a mounting frame, a first lifting component, a lifting frame, a second lifting component, a capping pin plate, a capping pin and a capping plate;
the first lifting assembly is arranged on the mounting frame; the lifting frame is arranged on the first lifting assembly, and the first lifting assembly drives the lifting frame to lift;
the second lifting assembly is arranged on the lifting frame, the binding pin plate is arranged on the second lifting assembly, the binding pin is arranged on the binding pin plate, the second lifting assembly drives the binding pin plate to lift, and the binding pin plate drives the binding pin to lift;
the gland plate is arranged on the lifting frame, the gland pin penetrates through the gland plate, and the gland pin and the gland plate can slide relatively.
Preferably, the first lifting assembly is a gland screw rod motor, and the gland screw rod motor drives the lifting frame to lift through a gland screw rod.
Preferably, the mounting rack is provided with a fixing plate, and the gland screw rod motor is arranged on the fixing plate.
Preferably, the second lifting assembly is a cap withdrawing screw rod motor, and the cap withdrawing screw rod motor drives the cap bundling pin plate to lift through the cap withdrawing screw rod.
Preferably, the lifting frame further comprises a guide assembly, the guide assembly is arranged on the mounting frame and the lifting frame, and the guide assembly is used for guiding the lifting frame in a lifting mode.
Preferably, the guide assembly comprises a lifting guide rod and a lifting guide block;
the lifting guide block is arranged on the mounting frame, a guide hole is formed in the lifting guide block, one end of the lifting guide rod is arranged on the lifting frame, and one end, far away from the linear bearing, of the lifting guide rod is located in the guide hole.
Preferably, the lifting guide rod comprises a guide rod and a linear bearing;
the linear bearing is arranged on the lifting frame, one end of the guide rod is connected and arranged on the linear bearing, and the other end of the guide rod is positioned in the guide hole.
Preferably, a lifting photoelectric switch is arranged on the mounting rack, a lifting light blocking sheet is arranged on the lifting rack, and the lifting photoelectric switch and the lifting light blocking sheet are matched for determining the lifting position of the lifting rack.
Preferably, the lifting frame is provided with a cover withdrawing photoelectric switch, the cover withdrawing light blocking plate is arranged on the cover binding pin plate, and the cover withdrawing photoelectric switch and the cover withdrawing light blocking plate are matched for confirming the position of the cover binding pin.
Preferably, the number of the binding studs is multiple, and the binding studs are uniformly arranged on the binding stud plate at intervals.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the utility model adopts the mutual cooperation of the gland screw rod motor and the cap withdrawing screw rod motor, and realizes the automatic bundling, moving and capping of the 96-hole silica gel cap by combining the cross lifting and staggering of the bundling pin and the gland plate;
2. the binding pin plate of the utility model is provided with a plurality of binding pins which are evenly distributed, thereby ensuring the flatness of the 96-hole silica gel cover in the moving process, ensuring 96 bulges on the 96-hole silica gel cover, aligning 96 reagent holes on the 96-hole amplification plate and ensuring the tightness of the PCR amplification process;
3. the utility model discloses a gland lead screw motor with move back the cooperation of lid lead screw motor, prick the lid pin and the gland board through the drive and go up and down, flexible in turn, realized getting the lid automatically, move lid, gland, move back the lid, realize providing the basis for PCR augmentations realizes automaticly.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic perspective view of an automatic capping device for a PCR amplification plate in an embodiment;
FIG. 2 is a front view of an automatic capping and capping apparatus for a PCR amplification plate in one embodiment;
FIG. 3 is a right side view of an automatic capping and capping apparatus for a PCR amplification plate in one embodiment;
FIG. 4 is a left side view of an automatic capping and capping apparatus for a PCR amplification plate in one embodiment;
FIG. 5 is a top view of an automatic capping apparatus for PCR amplification plates in one embodiment;
FIG. 6 is a bottom view of an automatic capping apparatus for a PCR amplification plate in one embodiment.
The figures show that:
196 hole silica gel covers 10 of mounting bracket
296-well amplification plate 11
Lifting light barrier 12 of gland screw rod motor 3
Gland screw 301 lifting photoelectric switch 13
Lifting frame 4 cover-removing photoelectric switch 14
Cover removing light-blocking sheet 15 of cover removing screw rod motor 5
Lifting guide rod 16 of cap removing screw rod 6
7 linear bearing 1601 of bundle cover pin board
Bundle cover pin 8 guide bar 1602
Gland plate 9 guide block 17
Detailed Description
The present invention will be described in detail with reference to the following embodiments. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that various changes and modifications can be made by one skilled in the art without departing from the spirit of the invention. All of which belong to the protection scope of the present invention.
Example 1:
as shown in fig. 1-6, this embodiment provides an automatic capping gland device that adds of PCR amplification board, including mounting bracket 1, first lifting unit 3, crane 4, second lifting unit 5, prick and cover pin board 7, prick and cover pin 8 and gland board 9, first lifting unit 3 sets up on mounting bracket 1, crane 4 sets up on first lifting unit 3, first lifting unit 3 drives crane 4 and goes up and down, second lifting unit 5 sets up on crane 4, prick and cover pin board 7 sets up on second lifting unit 5, prick and cover pin 8 and set up on prick and cover pin board 7, second lifting unit 5 drives prick and cover pin board 7 and goes up and down, prick and cover pin board 7 and drive and prick and cover pin 8 and go up and down, gland board 9 sets up on crane 4, prick and cover pin 8 and run through gland board 9, prick and cover pin 8 and gland board 9 between can relatively slide. The binding pintle 8 is provided in a plurality of numbers, and the binding pintle 8 is evenly arranged on the binding pintle plate 7 at intervals.
The mounting rack 1 is provided with a lifting photoelectric switch 13, the lifting frame 4 is provided with a lifting light barrier 12, the lifting photoelectric switch 13 and the lifting light barrier 12 are matched to determine the lifting position of the lifting frame 4, the lifting frame 4 is provided with a cover withdrawing photoelectric switch 14, the cover withdrawing light barrier 15 is arranged on the cover binding pin plate 7, and the cover withdrawing photoelectric switch 14 and the cover withdrawing light barrier 15 are matched to confirm the position of the cover binding pin 8.
The automatic capping and capping device that adds of PCR amplification board that this embodiment provided still includes the guide assembly, the guide assembly sets up on mounting bracket 1 and crane 4, the guide assembly is used for crane 4's lift direction, the guide assembly includes lift guide bar 16 and lift guide block 17, lift guide block 17 sets up on mounting bracket 1, be provided with the guiding hole on the lift guide block 17, lift guide bar 16's one end sets up on crane 4, lift guide bar 16 keeps away from linear bearing 1601's one end and is located the guiding hole, lift guide bar 16 includes guide bar 1602 and linear bearing 1601, linear bearing 1601 sets up on crane 4, the one end connection setting of guide bar 1602 is on linear bearing 1601, the other end of guide bar 1602 is located the guiding hole.
First lifting unit 3 is gland lead screw motor, and gland lead screw motor drives crane 4 through gland lead screw 301 and goes up and down, and mounting bracket 1 is provided with fixed plate 2, and gland lead screw motor sets up on fixed plate 2, and second lifting unit 5 is for moving back the lid lead screw motor, moves back the lid lead screw motor and drives bundle lid post cotter plate 7 through moving back lid lead screw 6 and go up and down.
The working principle is as follows:
taking a cover: the cover withdrawing screw rod motor is driven, when the cover binding pin 8 extends out of the lower part of the cover binding pin plate 7, the device integrally moves to the position above the 96-hole silica gel cover 10, the cover binding pin 8 is aligned to a pit on the 96-hole silica gel cover 10, the cover pressing screw rod motor is driven, the cover binding pin 8 is downwards inserted into the pit on the 96-hole silica gel cover 10 and is in interference fit with the pit, and the 96-hole silica gel cover 10 is taken up;
and (4) pressing and removing the cover: the device integrally moves to the position above the 96-hole amplification plate 11, the bulge below the 96-hole silica gel cover 10 is aligned to the pipe hole in the 96-hole amplification plate 11, the cover pressing screw rod motor is driven, the cover pressing plates 9 and the 96-hole silica gel cover 10 are downward at the same time, the 96-hole silica gel cover 10 is pressed on the 96-hole amplification plate 11, then the cover withdrawing screw rod motor is driven to be upward, the cover binding pin 8 is driven to retract upwards into the cover pressing plate 9, and the cover withdrawing function is realized.
Example 2:
those skilled in the art will understand this embodiment as a more specific description of embodiment 1.
As shown in fig. 1 to 6, the present embodiment provides an automatic capping and capping device for a PCR amplification plate, which includes a mounting frame 1, two lifting guide rods 16 fixed on the mounting frame 1 as a main body, and a capping screw rod motor installed on a motor fixing plate 2. The lifting frame 4 is installed on a linear bearing 1601 passing through the gland wire 301 and the two lifting guide rods 16. A cap withdrawing screw rod motor is arranged in the middle of the lifting frame 4, and a gland plate 9 is fixed at the bottom. And a cover binding pin plate 7 with a plurality of cover binding pins 8 is arranged at the bottom end of a cover removing screw rod 6 on the cover removing screw rod motor, and the cover binding pins 8 penetrate through a gland plate 9 to realize vertical guiding.
The mounting rack 1 is provided with a lifting photoelectric switch 13 which is matched with a lifting light barrier 12 fixed on the lifting rack 4 to determine the lifting position. The lifting frame 4 is provided with a cover withdrawing photoelectric switch 14 which is matched with a cover withdrawing light blocking sheet 15 on the cover binding pin plate 7 to determine the position of the cover binding pin 8 and realize the binding and withdrawing of the 96-hole silica gel cover 10.
The working principle is as follows:
taking a cover: the cover withdrawing screw rod motor is driven, when the cover binding pin 8 extends out of the lower part of the cover binding pin plate 7, the device integrally moves to the position above the 96-hole silica gel cover 10, the cover binding pin 8 is aligned to a pit on the 96-hole silica gel cover 10, the cover pressing screw rod motor is driven, the cover binding pin 08 is downwards inserted into the pit on the 96-hole silica gel cover 10 and is in interference fit with the pit, and the 96-hole silica gel cover 10 is taken up;
and (4) pressing and removing the cover: the device integrally moves to the position above the 96-hole amplification plate 11, the bulge below the 96-hole silica gel cover 10 is aligned to the pipe hole in the 96-hole amplification plate 11, the cover pressing screw rod motor is driven, the cover pressing plates 9 and the 96-hole silica gel cover 10 are downward at the same time, the 96-hole silica gel cover 10 is pressed on the 96-hole amplification plate 11, then the cover withdrawing screw rod motor is driven to be upward, the cover binding pin 8 is driven to retract upwards into the cover pressing plate 9, and the cover withdrawing function is realized.
The material of the 96-hole silica gel cover 10 determines the flexible state thereof, and increases the structural difficulty of automatic taking and placing. The device of this embodiment has set up a plurality of bundle lid pins 8 above bundle lid pin board 7, and evenly distributed, has guaranteed 96 hole silica gel lid 10 and at the roughness of removal in-process, guarantees 96 archs on the 96 hole silica gel lid 10, can both aim at 96 reagent holes on the 96 hole amplification board 11, has guaranteed the seal of PCR amplification process.
The utility model discloses a gland lead screw motor with move back the cooperation of lid lead screw motor, combine to prick the alternately going up and down of lid pin and gland board and fall by mistake, realize pricking the automation of 96 hole silica gel covers and get, remove and the gland.
In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.
The foregoing description of the specific embodiments of the invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.

Claims (10)

1. An automatic capping and capping device for a PCR amplification plate is characterized by comprising an installation frame (1), a first lifting component (3), a lifting frame (4), a second lifting component (5), a capping pin plate (7), a capping pin (8) and a capping plate (9);
the first lifting assembly (3) is arranged on the mounting frame (1); the lifting frame (4) is arranged on the first lifting component (3), and the first lifting component (3) drives the lifting frame (4) to lift;
the second lifting assembly (5) is arranged on the lifting frame (4), the binding pin plate (7) is arranged on the second lifting assembly (5), the binding pin (8) is arranged on the binding pin plate (7), the second lifting assembly (5) drives the binding pin plate (7) to lift, and the binding pin plate (7) drives the binding pin (8) to lift;
the gland plate (9) is arranged on the lifting frame (4), the gland pin (8) penetrates through the gland plate (9), and the gland pin (8) and the gland plate (9) can slide relatively.
2. The automatic capping and capping device for the PCR amplification plate of claim 1, wherein the first lifting component (3) is a capping screw motor, and the capping screw motor drives the lifting rack (4) to lift through a capping screw (301).
3. The automatic capping and capping device for PCR amplification plates according to claim 2, wherein the mounting rack (1) is provided with a fixing plate (2), and the capping screw motor is provided on the fixing plate (2).
4. The automatic capping and capping device for the PCR amplification plate according to claim 1, wherein the second lifting component (5) is a cap withdrawing screw rod motor, and the cap withdrawing screw rod motor drives the capping pin plate (7) to lift through a cap withdrawing screw rod (6).
5. The automatic capping and capping device for the PCR amplification plate according to claim 1, further comprising a guide assembly, wherein the guide assembly is arranged on the mounting rack (1) and the lifting rack (4), and the guide assembly is used for guiding the lifting rack (4) to lift.
6. The automatic capping and capping device for PCR amplification plates according to claim 5, wherein the guide assembly comprises an elevation guide rod (16) and an elevation guide block (17);
the lifting guide block (17) is arranged on the mounting rack (1), a guide hole is formed in the lifting guide block (17), one end of the lifting guide rod (16) is arranged on the lifting rack (4), and one end of the lifting guide rod (16) far away from the lifting rack (4) is located in the guide hole.
7. The automatic capping and capping apparatus for PCR amplification plates according to claim 6, wherein the elevation guide rod (16) comprises a guide rod (1602) and a linear bearing (1601);
the linear bearing (1601) is arranged on the lifting frame (4), one end of the guide rod (1602) is connected and arranged on the linear bearing (1601), and the other end of the guide rod (1602) is positioned in the guide hole.
8. The automatic capping and capping device for the PCR amplification plate according to claim 1, wherein a lifting photoelectric switch (13) is arranged on the mounting rack (1), a lifting light barrier (12) is arranged on the lifting rack (4), and the lifting photoelectric switch (13) and the lifting light barrier (12) are matched to determine the lifting position of the lifting rack (4).
9. The automatic capping and capping device for the PCR amplification plate according to claim 1, wherein the lifting frame (4) is provided with a cap withdrawing photoelectric switch (14), the capping pin plate (7) is provided with a cap withdrawing light-blocking sheet (15), and the cap withdrawing photoelectric switch (14) and the cap withdrawing light-blocking sheet (15) are matched to confirm the position of the capping pin (8).
10. The automatic capping and capping device for PCR amplification plates according to claim 1, wherein the capping pins (8) are provided in plurality, and the capping pins (8) are uniformly spaced on the capping pin plate (7).
CN202221011552.9U 2022-04-28 2022-04-28 Automatic capping and capping device for PCR amplification plate Active CN217516649U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202221011552.9U CN217516649U (en) 2022-04-28 2022-04-28 Automatic capping and capping device for PCR amplification plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202221011552.9U CN217516649U (en) 2022-04-28 2022-04-28 Automatic capping and capping device for PCR amplification plate

Publications (1)

Publication Number Publication Date
CN217516649U true CN217516649U (en) 2022-09-30

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ID=83371342

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202221011552.9U Active CN217516649U (en) 2022-04-28 2022-04-28 Automatic capping and capping device for PCR amplification plate

Country Status (1)

Country Link
CN (1) CN217516649U (en)

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GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20221115

Address after: Room 120, Building 18, No. 1625, Caobao Road, Minhang District, Shanghai, 200235

Patentee after: Shanghai Shuoying Biotechnology Co.,Ltd.

Address before: No. 837, Yaocheng Avenue, Taizhou, Jiangsu 225300

Patentee before: JIANGSU BIOPERFECTUS TECHNOLOGIES CO.,LTD.