CN217351392U - Experimental cavity device for vascular culture - Google Patents
Experimental cavity device for vascular culture Download PDFInfo
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- CN217351392U CN217351392U CN202122916810.XU CN202122916810U CN217351392U CN 217351392 U CN217351392 U CN 217351392U CN 202122916810 U CN202122916810 U CN 202122916810U CN 217351392 U CN217351392 U CN 217351392U
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Abstract
The utility model belongs to the biomedical engineering field, provides a blood vessel culture experimental cavity device capable of repeating high temperature sterilization, uses a blood vessel connecting pipe with adjustable length and a convenient blood vessel installation device, provides an experimental cavity device which can realize the in vitro simulated culture of blood vessels and can be used for simulating the blood flow stimulation to the blood vessels; the blood vessel assembly is realized through an external fixing device, so that the blood vessel is convenient to assemble and easy to implement, and the pollution risk during operation is reduced; meanwhile, the blood vessel is placed into the blood vessel culture cavity in a pressing-in mode.
Description
Technical Field
The utility model belongs to the biomedical engineering field relates to a vascular cultivation's for experiments laboratory chamber device, provides the technical scheme that can be used to the blood vessel simulation blood flow amazing, realizes the experimental chamber device of the external simulation cultivation of blood vessel.
Background
Experimental systems for blood vessel culture are available, but culture chambers are prone to contamination during the blood vessel assembly process.
The poor tightness of the operation of placing the blood vessel into the blood vessel cavity after the blood vessel is assembled leads to the circulation inside and outside the blood vessel.
Disclosure of Invention
In order to solve the above problems, there is provided a solution, a laboratory cavity apparatus for blood vessel culture; the blood vessel culture cavity comprises a blood vessel culture cavity 1, wherein the blood vessel culture cavity consists of a main body cavity 1-1 and a cavity cover 1-2, and a sealing strip is arranged on the cavity cover 1-2, so that the cavity cover and the main body cover are not sealed after being fixed; an internal circulation joint 1-4, an external circulation joint 1-5 of a blood vessel and a reserved joint are fixed outside the main body cavity; a pair of blood vessel joints 2 are fixed in the main body cavity 1-1, and each blood vessel joint 2 consists of a blood vessel sealing fixing piece 2-1, a blood vessel joint pressing piece (2-2), an elastic piece 2-3, an upper sealing ring 2-4 of the blood vessel sealing fixing piece and a blood vessel connecting pipe 2-5; when a blood vessel is loaded, the blood vessel is provided with two opposite blood vessel joints through a blood vessel assembling device 3, the blood vessel assembling device consists of a bottom plate 3-1 and a magnetic seat 3-2, the magnetic seat is absorbed on the bottom plate, the magnetic seat penetrates through an adjusting plate 3-3 to be connected with the blood vessel joints 2, the pair of blood vessel joints are inserted into a blood vessel culture cavity after the blood vessel is loaded or simulated, and then the blood vessel assembling device is taken down; covering a cavity cover; each joint on the blood vessel culture cavity main body is used for connecting an external internal circulation pipeline and an external circulation pipeline.
Furthermore, the materials used by the blood vessel culture cavity and the blood vessel joint are biocompatible materials, and the materials are PEEK or titanium alloy or stainless steel materials; the cavity cover material is PC or transparent glass high-temperature resistant material; the elastic piece is made of high-temperature and high-pressure materials.
Further, the main body cavity 1-1 is designed with a blood vessel joint 2 to realize the sealing between the blood vessel joint and the main body cavity in a press-in type mode.
Furthermore, the vascular joint 2 compresses the elastic part 2-3 by adjusting the vascular joint compressing part 2-2 to form compression fixation on the vascular connecting pipe 2-5, and when the vascular connecting pipe is not compressed and fixed, the vascular connecting pipe can be loosened by adjusting the vascular compressing part to adjust the extending length of the connecting pipe in the vascular joint; the end of the blood vessel connecting pipe 2-5 connected with the blood vessel is provided with a round opening and a dent for guiding and tightening the blood vessel; the wall thickness of the blood vessel connecting pipe wall is 0.1mm-0.5 mm.
Further, in the blood vessel assembling device, a magnetic piece is fixed in a magnetic base; the bottom plate is made of a magnetic adsorbable material, and meanwhile, the size of the bottom plate can ensure that the assembly is not overturned during the assembly of the blood vessel;
the two magnetic force seats penetrate through the adjusting plate to be connected with the blood vessel joint, one end of the adjusting plate can be rotatably adjusted, the other end of the adjusting plate can be rotatably adjusted and the distance between the magnetic force seats and the other end of the adjusting plate can be adjusted, so that the blood vessel can be conveniently assembled; the maximum distance of the magnetic base which can be adjusted by the bottom plate is the size of the blood vessel which is arranged in the main body cavity, and after the blood vessel is assembled, the blood vessel assembling device presses the blood vessel joint which is provided with the blood vessel into the main body cavity together.
Further, the blood vessel is inoculated into the internal and external circulation to carry out the circulation culture of the blood vessel, and the blood vessel is placed in a CO2 incubator or is kept at a constant temperature and the PH of the culture solution is stable in the culture environment to carry out the blood vessel culture.
The utility model relates to an experimental cavity device for vascular culture's beneficial effect is:
1. the invention greatly reduces the operation risk of pollution in the assembling process of the blood vessel;
2. the invention simplifies the sealing operation of placing the blood vessel into the blood vessel cavity after the blood vessel is assembled;
3. the invention makes it easy to place the blood vessel into the blood vessel culture cavity.
Therefore, the utility model provides a device of a blood vessel assembly and a blood vessel culture cavity for in vitro blood vessel culture.
Drawings
FIG. 1 is an illustration of an experimental cavity device for blood vessel culture
FIG. 2 is a diagram of a culture cavity of a blood vessel culture experimental cavity device
FIG. 3 is a main body cavity diagram of a culture cavity of a blood vessel culture experiment cavity device
FIG. 4 is a diagram of a vessel joint of a vessel culture experimental cavity device
FIG. 5 is a diagram of a blood vessel assembly device of a blood vessel culture experiment cavity device
FIG. 6 is a blood vessel assembly drawing of a blood vessel culture experiment cavity device
FIG. 7 is a blood vessel assembly drawing of a blood vessel culture experiment cavity device
FIG. 8 is a diagram of a blood vessel assembly device adjusting plate of a blood vessel culture experiment cavity device
FIG. 9 is a drawing of a vessel joint and vessel assembly into a vessel culture cavity of a vessel culture experimental cavity device
FIG. 10 is a diagram of a blood vessel culture cavity of a blood vessel culture experimental cavity device
FIG. 11 is a schematic view of a circulatory system of a vascular culture experimental cavity device.
Description of numbering: 1 blood vessel culture cavity, 1-1 blood vessel culture cavity is composed of main body cavity, 1-2 cavity covers, 1-3 blood vessel internal circulation joints, 1-4 blood vessel external circulation joints, 2 blood vessel joints, 2-1 blood vessel sealing fixing parts, 2-2 blood vessel joint pressing parts, 2-3 elastic parts, 2-4 sealing rings and 2-5 blood vessel connecting pipes; 3, a blood vessel assembling device, 3-1, a blood vessel assembling device and a blood vessel assembling method, wherein the blood vessel assembling device comprises a bottom plate, a 3-2 magnetic base, a 3-3 adjusting plate and 4 blood vessels; a guide B is indented, a pipe hole D is formed in the pipe hole C, a long hole F is formed in the pipe joint, a P pressure gauge is fixed on the pipe joint, and the distance of the pipe joint L in the blood vessel culture cavity is measured.
Detailed Description
A laboratory cavity device for blood vessel culture; the blood vessel culture cavity comprises a blood vessel culture cavity 1, wherein the blood vessel culture cavity consists of a main body cavity 1-1 and a cavity cover 1-2, and a sealing strip is arranged on the cavity cover 1-2, so that the cavity cover and the main body cover are not sealed after being fixed; an internal circulation joint 1-4, an extravascular circulation joint 1-5 and a reserved joint are fixed outside the cavity of the main body; a pair of blood vessel joints 2 are fixed in the main body cavity 1-1, and the blood vessel joints 2 consist of a blood vessel sealing fixing piece 2-1, a blood vessel joint pressing piece 2-2, an elastic piece 2-3, an upper sealing ring 2-4 of the blood vessel sealing fixing piece and a blood vessel connecting pipe 2-5; when a blood vessel is loaded, the blood vessel is provided with two opposite blood vessel joints through a blood vessel assembling device 3, the blood vessel assembling device consists of a bottom plate 3-1 and a magnetic seat 3-2, the magnetic seat is absorbed on the bottom plate, the magnetic seat penetrates through an adjusting plate 3-3 to be connected with the blood vessel joints 2, after the blood vessel is loaded or simulated on a pair of blood vessel joints, the blood vessel assembling device is inserted into a blood vessel culture cavity, and then taken down; covering a cavity cover; each joint on the blood vessel culture cavity main body is used for connecting an external internal circulation pipeline and an external circulation pipeline.
Further, the materials used by the blood vessel culture cavity and the blood vessel joint are biocompatible materials, and the materials are PEEK or titanium alloy or stainless steel materials; the cavity cover material is PC or transparent glass high-temperature resistant material; the elastic piece is made of a silica gel material.
Further, the main body cavity 1-1 is designed with a blood vessel joint 2 to realize the sealing between the blood vessel joint and the main body cavity in a press-in type mode.
Further, the blood vessel joint 2 is pressed and fixed on the blood vessel connecting pipe 2-5) by adjusting the blood vessel joint pressing piece 2-2 to press the elastic piece 2-3, when the blood vessel connecting pipe is not pressed and fixed, the blood vessel connecting pipe can be loosened by adjusting the blood vessel pressing piece, and the extending length of the connecting pipe in the blood vessel joint is adjusted; the end of the blood vessel connecting pipe 2-5 connected with the blood vessel is provided with a round opening and a dent for guiding and tightening the blood vessel; the wall thickness of the blood vessel connecting pipe wall is 0.1mm-0.5 mm.
Further, in the blood vessel assembling device, a magnetic piece is fixed in a magnetic base; the bottom plate is made of a magnetic adsorbable material, and meanwhile, the size of the bottom plate can ensure that the assembly is not overturned during blood vessel assembly; the two magnetic force seats penetrate through the adjusting plate to be connected with the blood vessel joint, one end of the adjusting plate can be rotatably adjusted, the other end of the adjusting plate can be rotatably adjusted and the distance between the magnetic force seats and the other end of the adjusting plate can be adjusted, so that the blood vessel can be conveniently assembled; the maximum distance of the magnetic base with the adjustable bottom plate is the size of the magnetic base arranged in the main body cavity, and after the blood vessel is assembled, the blood vessel assembly device presses the blood vessel joint with the blood vessel into the main body cavity together.
Further, the blood vessels are inoculated into the internal and external circulation for circulation culture, and then are placed in a CO2 incubator or kept at a constant temperature and the pH of the culture solution is stabilized in a culture environment.
Claims (5)
1. A blood vessel culture experiment cavity device is characterized by comprising a blood vessel culture cavity (1), wherein the blood vessel culture cavity consists of a main body cavity (1-1) and a cavity cover (1-2), and a sealing strip is arranged on the cavity cover (1-2) to ensure that the cavity cover and the main body cover are not sealed after being fixed; an internal circulation joint (1-4), an external circulation joint (1-5) of the blood vessel and a reserved joint are fixed outside the cavity of the main body; a pair of blood vessel joints (2) are fixed in the main body cavity (1-1), and each blood vessel joint (2) consists of a blood vessel sealing fixing piece (2-1), a blood vessel joint pressing piece (2-2), an elastic piece (2-3), an upper sealing ring (2-4) of the blood vessel sealing fixing piece and a blood vessel connecting pipe (2-5); when a blood vessel is loaded, the blood vessel is provided with two opposite blood vessel joints through a blood vessel assembling device (3), the blood vessel assembling device consists of a bottom plate (3-1) and a magnetic seat (3-2), the magnetic seat is attracted on the bottom plate, the magnetic seat penetrates through an adjusting plate (3-3) to be connected with the blood vessel joints (2), the pair of blood vessel joints are inserted into a blood vessel culture cavity after the blood vessel or a simulated blood vessel is loaded on the blood vessel joints, and then the blood vessel assembling device is taken down; covering a cavity cover; each joint on the blood vessel culture cavity main body is used for connecting an external internal circulation pipeline and an external circulation pipeline.
2. The vessel culture experimental cavity device of claim 1, wherein the vessel culture cavity and the vessel joint are made of biocompatible materials, such as PEEK, titanium alloy or stainless steel; the cavity cover material is PC or transparent glass high-temperature resistant material; the elastic piece is made of a material capable of realizing high temperature and high pressure.
3. The vessel culture experiment cavity device as claimed in claim 1, wherein the main body cavity (1-1) is designed with a vessel joint (2) to seal the main body cavity by pressing in.
4. The vessel culture experimental cavity device according to claim 1, characterized in that the vessel joint (2) is pressed and fixed to the vessel connecting pipe (2-5) by adjusting the vessel joint pressing member (2-2) to press the elastic member (2-3), and when the vessel connecting pipe is not pressed and fixed, the vessel connecting pipe can be loosened by adjusting the vessel pressing member to adjust the length of the connecting pipe extending out of the vessel joint; the end of the blood vessel connecting pipe (2-5) connected with the blood vessel is provided with a round opening and a dent for guiding and tightening the blood vessel; the wall thickness of the blood vessel connecting pipe wall is 0.1mm-0.5 mm.
5. The vessel culture experimental cavity device of claim 1, wherein a magnetic member is fixed in the magnetic base; the bottom plate is made of a magnetic adsorbable material, and the size of the bottom plate is required to ensure that the assembly is not overturned during the assembly of the blood vessel; the two magnetic force seats penetrate through the adjusting plate to be connected with the blood vessel joint, the magnetic force seats can be rotatably adjusted at one end of the adjusting plate, and can be rotatably adjusted at the other end and adjusted in distance from the magnetic force seats at the other end, so that the blood vessel can be conveniently assembled; the maximum distance of the magnetic base with the adjustable bottom plate is the size of the magnetic base arranged in the main body cavity, and after the blood vessel is assembled, the blood vessel assembly device presses the blood vessel joint with the blood vessel into the main body cavity together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122916810.XU CN217351392U (en) | 2021-11-25 | 2021-11-25 | Experimental cavity device for vascular culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122916810.XU CN217351392U (en) | 2021-11-25 | 2021-11-25 | Experimental cavity device for vascular culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217351392U true CN217351392U (en) | 2022-09-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122916810.XU Active CN217351392U (en) | 2021-11-25 | 2021-11-25 | Experimental cavity device for vascular culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217351392U (en) |
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2021
- 2021-11-25 CN CN202122916810.XU patent/CN217351392U/en active Active
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