CN114107049A - Experimental cavity device for blood vessel culture - Google Patents
Experimental cavity device for blood vessel culture Download PDFInfo
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- CN114107049A CN114107049A CN202111414058.7A CN202111414058A CN114107049A CN 114107049 A CN114107049 A CN 114107049A CN 202111414058 A CN202111414058 A CN 202111414058A CN 114107049 A CN114107049 A CN 114107049A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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Abstract
The invention belongs to the field of biomedical engineering, and provides a blood vessel culture experimental cavity device capable of achieving repeated high-temperature sterilization, which uses a blood vessel connecting pipe with adjustable length and a convenient blood vessel installation device, and provides an experimental cavity device for realizing in-vitro simulated culture of blood vessels and simulating blood flow stimulation on the blood vessels; the blood vessel assembly is realized through an external fixing device, so that the blood vessel is convenient to assemble and easy to implement, and the pollution risk during operation is reduced; meanwhile, the blood vessel is placed into the blood vessel culture cavity in a pressing-in mode.
Description
Technical Field
The invention belongs to the field of biomedical engineering, relates to an experimental cavity device for experimental blood vessel culture, and provides a technical scheme of an easy-to-operate experimental cavity device for in-vitro simulation culture of blood vessels.
Background
Experimental systems for blood vessel culture are available, but culture chambers are prone to contamination during the blood vessel assembly process.
The poor tightness of the operation of placing the blood vessel into the blood vessel cavity after the blood vessel is assembled leads to the circulation inside and outside the blood vessel.
Disclosure of Invention
The poor tightness of the operation of placing the blood vessel into the blood vessel cavity after the blood vessel is assembled leads to the circulation inside and outside the blood vessel.
In order to solve the above problems, there is provided a solution, a laboratory cavity apparatus for blood vessel culture; the blood vessel culture device comprises a blood vessel culture cavity (1), wherein the blood vessel culture cavity consists of a main body cavity (1-1) and a cavity cover (1-2), and a sealing strip is arranged on the cavity cover (1-2) to ensure that the cavity cover and the main body cover are not sealed after being fixed; an internal circulation joint (1-4), an external circulation joint (1-5) of the blood vessel and a reserved joint are fixed outside the cavity of the main body; a pair of blood vessel joints (2) are fixed in the main body cavity (1-1), and each blood vessel joint (2) consists of a blood vessel sealing fixing piece (2-1), a blood vessel joint pressing piece (2-2), an elastic piece (2-3), an upper sealing ring (2-4) of the blood vessel sealing fixing piece and a blood vessel connecting pipe (2-5); when a blood vessel is loaded, the blood vessel is provided with two opposite blood vessel joints through a blood vessel assembling device (3), the blood vessel assembling device comprises a bottom plate (3-1) and a magnetic seat (3-2), the magnetic seat is attracted on the bottom plate and penetrates through an adjusting plate (3-3) to be connected with the blood vessel joints (2), the pair of blood vessel joints are inserted into a blood vessel culture cavity after the blood vessel or a simulated blood vessel is loaded on the blood vessel joints, and then the blood vessel assembling device is taken down; covering a cavity cover; each joint on the blood vessel culture cavity main body is used for connecting an external internal circulation pipeline and an external circulation pipeline.
Furthermore, the blood vessel culture cavity and the blood vessel joint are made of biocompatible materials such as PEEK, titanium alloy and stainless steel; the cavity cover material is PC or transparent glass high-temperature resistant material; the elastic part is made of high-temperature and high-pressure materials, such as: silica gel.
Further, the main body cavity (1-1) is designed with a blood vessel joint (2) to realize sealing between the blood vessel joint and the main body cavity in a press-in type mode.
Furthermore, the blood vessel joint (2) compresses the elastic part (2-3) through adjusting the blood vessel joint compressing part (2-2) to form compressing and fixing on the blood vessel connecting pipe (2-5), and when the blood vessel connecting pipe is not compressed and fixed, the blood vessel connecting pipe can be loosened through adjusting the blood vessel compressing part to adjust the extending length of the connecting pipe in the blood vessel joint; the end of the blood vessel connecting pipe (2-5) connected with the blood vessel is provided with a round opening and a dent for guiding and tightening the blood vessel; the wall thickness of the blood vessel connecting pipe wall is 0.1mm-0.5 mm.
Further, in the blood vessel assembling device, a magnetic part is fixed in a magnetic base; the bottom plate is made of a magnetic adsorbable material, and the size of the bottom plate can ensure that the assembly is not turned over during blood vessel assembly;
the two magnetic force seats penetrate through the adjusting plate to be connected with the blood vessel joint, the magnetic force seats can be rotatably adjusted at one end of the adjusting plate, and can be rotatably adjusted at the other end and adjusted in distance from the magnetic force seats at the other end, so that the blood vessel can be conveniently assembled; the maximum distance of the magnetic base with the adjustable bottom plate is the size of the magnetic base arranged in the main body cavity, and after the blood vessel is assembled, the blood vessel assembly device presses the blood vessel joint with the blood vessel into the main body cavity together.
Further, the blood vessel is inoculated into the internal and external circulation to carry out the circulation culture of the blood vessel, and the blood vessel is placed in a CO2 incubator or is kept at a constant temperature and the PH of the culture solution is stable in the culture environment to carry out the blood vessel culture.
The experimental cavity device for blood vessel culture has the beneficial effects that:
1. the invention greatly reduces the operation risk of pollution in the assembling process of the blood vessel;
2. the invention simplifies the sealing operation of placing the blood vessel into the blood vessel cavity after the blood vessel is assembled;
3. the invention makes it easy to place the blood vessel into the blood vessel culture cavity.
The invention thus provides a novel vascular assembly and vascular culture chamber device for in vitro vascular culture.
Brief description of the drawings
FIG. 1 is an illustration of an exemplary laboratory device for vascular culture
FIG. 2 is a diagram of a culture chamber of an experimental chamber device for blood vessel culture
FIG. 3 is a main body cavity diagram of a culture cavity of an experimental cavity device for blood vessel culture
FIG. 4 is a diagram of a vessel junction of an experimental cavity device for blood vessel culture
FIG. 5 is a diagram of a blood vessel assembling device of an experimental cavity device for blood vessel culture
FIG. 6 is a blood vessel assembly drawing of a laboratory cavity device for blood vessel culture
FIG. 7 is a blood vessel assembly drawing of a laboratory cavity device for blood vessel culture
FIG. 8 is a diagram of an experimental chamber device adjusting plate for vascular culture
FIG. 9 is a diagram of the experimental cavity device vessel joint for vessel culture and the vessel assembly into the vessel culture cavity
FIG. 10 is a diagram of a blood vessel culture cavity of an experimental cavity device for blood vessel culture
FIG. 11 is a schematic view of a circulatory system using a laboratory vessel apparatus for blood vessel culture.
Illustration of the drawings: 1 blood vessel culture cavity, 1-1 blood vessel culture cavity is composed of main body cavity, 1-2 cavity covers, 1-3 blood vessel internal circulation joints, 1-4 blood vessel external circulation joints, 2 blood vessel joints, 2-1 blood vessel sealing fixing parts, 2-2 blood vessel joint pressing parts, 2-3 elastic parts, 2-4 sealing rings and 2-5 blood vessel connecting pipes; 3, a blood vessel assembling device, 3-1, a blood vessel assembling device and a blood vessel assembling method, wherein the blood vessel assembling device comprises a bottom plate, a 3-2 magnetic base, a 3-3 adjusting plate and 4 blood vessels; the guide A guides the distance of the vascular joint L in the vascular culture cavity, the indent B guides the vascular hole D, the long hole F, the vascular joint fixing clamp P and the pressure gauge L.
Detailed Description
Examples
A laboratory cavity device for blood vessel culture; the blood vessel culture device comprises a blood vessel culture cavity (1), wherein the blood vessel culture cavity consists of a main body cavity (1-1) and a cavity cover (1-2), and a sealing strip is arranged on the cavity cover (1-2) to ensure that the cavity cover and the main body cover are not sealed after being fixed; an internal circulation joint (1-4), an external circulation joint (1-5) of the blood vessel and a reserved joint are fixed outside the cavity of the main body; a pair of blood vessel joints (2) are fixed in the main body cavity (1-1), and each blood vessel joint (2) consists of a blood vessel sealing fixing piece (2-1), a blood vessel joint pressing piece (2-2), an elastic piece (2-3), an upper sealing ring (2-4) of the blood vessel sealing fixing piece and a blood vessel connecting pipe (2-5); when a blood vessel is loaded, the blood vessel is provided with two opposite blood vessel joints through a blood vessel assembling device (3), the blood vessel assembling device comprises a bottom plate (3-1) and a magnetic seat (3-2), the magnetic seat is attracted on the bottom plate and penetrates through an adjusting plate (3-3) to be connected with the blood vessel joints (2), the pair of blood vessel joints are inserted into a blood vessel culture cavity after the blood vessel or a simulated blood vessel is loaded on the blood vessel joints, and then the blood vessel assembling device is taken down; covering a cavity cover; each joint on the blood vessel culture cavity main body is used for connecting an external internal circulation pipeline and an external circulation pipeline.
Furthermore, the blood vessel culture cavity and the blood vessel joint are made of biocompatible materials such as PEEK, titanium alloy and stainless steel; the cavity cover material is PC or transparent glass high-temperature resistant material; the elastic part is made of high-temperature and high-pressure materials, such as: silica gel.
Further, the main body cavity (1-1) is designed with a blood vessel joint (2) to realize sealing between the blood vessel joint and the main body cavity in a press-in type mode.
Furthermore, the blood vessel joint (2) compresses the elastic part (2-3) through adjusting the blood vessel joint compressing part (2-2) to form compressing and fixing on the blood vessel connecting pipe (2-5), and when the blood vessel connecting pipe is not compressed and fixed, the blood vessel connecting pipe can be loosened through adjusting the blood vessel compressing part to adjust the extending length of the connecting pipe in the blood vessel joint; the end of the blood vessel connecting pipe (2-5) connected with the blood vessel is provided with a round opening and a dent for guiding and tightening the blood vessel; the wall thickness of the blood vessel connecting pipe wall is 0.1mm-0.5 mm.
Further, in the blood vessel assembling device, a magnetic part is fixed in a magnetic base; the bottom plate is made of a magnetic adsorbable material, and the size of the bottom plate can ensure that the assembly is not turned over during blood vessel assembly; the two magnetic force seats penetrate through the adjusting plate to be connected with the blood vessel joint, the magnetic force seats can be rotatably adjusted at one end of the adjusting plate, and can be rotatably adjusted at the other end and adjusted in distance from the magnetic force seats at the other end, so that the blood vessel can be conveniently assembled; the maximum distance of the magnetic base with the adjustable bottom plate is the size of the magnetic base arranged in the main body cavity, and after the blood vessel is assembled, the blood vessel assembly device presses the blood vessel joint with the blood vessel into the main body cavity together.
Further, the blood vessels are inoculated into the internal and external circulation for circulation culture, and then are placed in a CO2 incubator or kept at a constant temperature and the pH of the culture solution is stabilized in a culture environment.
Claims (5)
1. An experimental cavity device for blood vessel culture is characterized by comprising a blood vessel culture cavity (1), wherein the blood vessel culture cavity consists of a main body cavity (1-1) and a cavity cover (1-2), and a sealing strip is arranged on the cavity cover (1-2) to ensure that the cavity cover and the main body cover are not sealed after being fixed; an internal circulation joint (1-4), an external circulation joint (1-5) of the blood vessel and a reserved joint are fixed outside the cavity of the main body; a pair of blood vessel joints (2) are fixed in the main body cavity (1-1), and each blood vessel joint (2) consists of a blood vessel sealing fixing piece (2-1), a blood vessel joint pressing piece (2-2), an elastic piece (2-3), an upper sealing ring (2-4) of the blood vessel sealing fixing piece and a blood vessel connecting pipe (2-5); when a blood vessel is loaded, the blood vessel is provided with two opposite blood vessel joints through a blood vessel assembling device (3), the blood vessel assembling device comprises a bottom plate (3-1) and a magnetic seat (3-2), the magnetic seat is attracted on the bottom plate and penetrates through an adjusting plate (3-3) to be connected with the blood vessel joints (2), the pair of blood vessel joints are inserted into a blood vessel culture cavity after the blood vessel or a simulated blood vessel is loaded on the blood vessel joints, and then the blood vessel assembling device is taken down; covering a cavity cover; each joint on the blood vessel culture cavity main body is used for connecting an external internal circulation pipeline and an external circulation pipeline.
2. The experimental cavity device for blood vessel culture as claimed in claim 1, wherein the blood vessel culture cavity and the blood vessel joint are made of biocompatible materials such as PEEK, titanium alloy and stainless steel; the cavity cover material is PC or transparent glass high-temperature resistant material; the elastic part is made of high-temperature and high-pressure materials, such as: silica gel.
3. Laboratory cavity device for blood vessel culture according to claim 1, characterized in that the main body cavity (1-1) is designed with a blood vessel joint (2) to achieve sealing between the blood vessel joint and the main body cavity by means of press-in.
4. The laboratory cavity device for blood vessel culture according to claim 1, wherein the blood vessel joint (2) is formed to be fixed to the blood vessel connection pipe (2-5) by adjusting the blood vessel joint compressing member (2-2) to compress the elastic member (2-3), and when the blood vessel connection pipe is not fixed to be compressed, the blood vessel connection pipe can be released by adjusting the blood vessel compressing member to adjust the length of the connection pipe extending out of the blood vessel joint; the end of the blood vessel connecting pipe (2-5) connected with the blood vessel is provided with a round opening and a dent for guiding and tightening the blood vessel; the wall thickness of the blood vessel connecting pipe wall is 0.1mm-0.5 mm.
5. The laboratory cavity device for blood vessel culture according to claim 1, wherein a magnetic member is fixed in the magnetic base; the bottom plate is made of a magnetic adsorbable material, and the size of the bottom plate can ensure that the assembly is not turned over during blood vessel assembly; the two magnetic force seats penetrate through the adjusting plate to be connected with the blood vessel joint, the magnetic force seats can be rotatably adjusted at one end of the adjusting plate, and can be rotatably adjusted at the other end and adjusted in distance from the magnetic force seats at the other end, so that the blood vessel can be conveniently assembled; the maximum distance of the magnetic base with the adjustable bottom plate is the size of the magnetic base arranged in the main body cavity, and after the blood vessel is assembled, the blood vessel assembly device presses the blood vessel joint with the blood vessel into the main body cavity together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111414058.7A CN114107049A (en) | 2021-11-25 | 2021-11-25 | Experimental cavity device for blood vessel culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111414058.7A CN114107049A (en) | 2021-11-25 | 2021-11-25 | Experimental cavity device for blood vessel culture |
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| Publication Number | Publication Date |
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| CN114107049A true CN114107049A (en) | 2022-03-01 |
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| CN202111414058.7A Pending CN114107049A (en) | 2021-11-25 | 2021-11-25 | Experimental cavity device for blood vessel culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232750A (en) * | 2022-09-22 | 2022-10-25 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use thereof |
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2021
- 2021-11-25 CN CN202111414058.7A patent/CN114107049A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232750A (en) * | 2022-09-22 | 2022-10-25 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use thereof |
| CN115232750B (en) * | 2022-09-22 | 2023-02-24 | 海迈医疗科技(苏州)有限公司 | Sterile culture bag and method of use |
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