CN109370893A - Biological culture reaction unit - Google Patents
Biological culture reaction unit Download PDFInfo
- Publication number
- CN109370893A CN109370893A CN201811294073.0A CN201811294073A CN109370893A CN 109370893 A CN109370893 A CN 109370893A CN 201811294073 A CN201811294073 A CN 201811294073A CN 109370893 A CN109370893 A CN 109370893A
- Authority
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- Prior art keywords
- culture
- silica membrane
- gel silica
- lower platen
- pressure
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 111
- 239000012528 membrane Substances 0.000 claims abstract description 53
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 9
- 239000000499 gel Substances 0.000 description 35
- 210000004027 cell Anatomy 0.000 description 10
- 230000006835 compression Effects 0.000 description 9
- 238000007906 compression Methods 0.000 description 9
- 239000003921 oil Substances 0.000 description 5
- 239000000741 silica gel Substances 0.000 description 5
- 229910002027 silica gel Inorganic materials 0.000 description 5
- 108010066278 cabin-4 Proteins 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010066057 cabin-1 Proteins 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000001459 mortal effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of biological culture reaction units, including culture cabin, pressure-applying unit, the pressure-applying unit to be made of inner ram, top board, lower platen, pressure spring；The inner ram upper surface is fixedly connected with top board with bolt, inner ram lower end is socketed with lower platen, and inner ram lower part is equipped with boss, and lower platen through-hole upper end is equipped with bulge loop, lower platen cooperates axially position by bulge loop and inner ram lower boss, move together lower platen can with inner ram upwards；Pressure spring is socketed with outside inner ram, the both ends of pressure spring are connected with top board, lower platen respectively.Inner ram and lower platen are connected with the gel silica membrane pressure touching in culture cabin respectively, wherein, lower platen makes closely to seal between gel silica membrane and culture tank body upper surface, inner ram applies the pressure of setting by gel silica membrane to the intracorporal liquid of culture tank for compressing gel silica membrane and culture tank body upper surface.The present invention can be used for automatic biological reaction experiment instrument, and can reduce the pollution for carrying out autoreactor to greatest extent.
Description
Technical field
The present invention relates to a kind of bioreactor, especially a kind of biological culture reactor belongs to bioreactor technology
Field.
Background technique
Bioreactor is the experiment tool for cultivating tissue of biological cells, is provided daily 1 hour for cell tissue
High pressure culture environment, it is necessary to meet cell and tissue structrue need stringent aseptic condition (once contamination of cells tissue will be by
To irremediable a mortal blow, sterile this is cell culture basis), sufficient nutrient environment (including (various types of cells must for nutrition
Must biotic factor degradation rate it is fast) supply and waste removing), for meet biological tissue needs routinely change every other day
Liquid.
The integrated bioreactor of the existing circulatory system and compression system.Such as patent publication No. CN103589638B,
A kind of pneumatic self-loopa animal cell culture bioreactor and its application method, positioned at big pot bottom magnetic agitation system,
Heating system and control system, big tank upper end are provided with sealing cover, and big tank two sides are provided with the collet connecting with heating system, small
Tank upper end is provided with end socket and its lower end is provided with opening, and end socket and sealing cover are sealedly and fixedly connected by snorkel, snorkel
Upper end is pierced by sealing cover and is connected separately with vacuum pump and air pressure pump, and lower end is pierced by end socket and enters in canister.Such as
Patent publication No. CN102899250B discloses a kind of controlled hydraulic bioreactor for organizational project, the upper end of top cover
Portion is provided with controlled hydraulic system, and the lower part of tank body is provided with circumfusion system；Be provided with flexible diaphragm in tank body, it is flexible every
The cavity that tank body and top cover are formed is separated into culture chamber and pressurizing chamber by film；Using hydraulic pressurization mode and controllable unloading technology,
It can be achieved to realize a variety of stimulus modalities to real-time, the precision control of the key parameters such as Pressure stimulation intensity, frequency, action time
Conversion；Culture solution import and export is introduced in culture systems to realize circumfusion culture.
The circulatory system of the integrated bioreactor of the existing circulatory system and compression system, culture tank body and the bottle that reloads
It is linked by silicone tube, peristaltic pump provides circulation power.It causes and changes liquid and (change liquid within 2 weeks) not in time, culture environment is unfavorable for cell
Tissue cultures.It is high that the circulatory system of multicomponent causes cell tissue pollution rate when changing liquid.The link complexity of various pipes links
Increase pollution risk.The existing circulatory system and combining together of pressure system bioreactor, so that compression chamber is long-term
One group of experiment occupies for a long time, and reactor service efficiency is low.Integrated design is not easy to the observation on Growth to sample, integration
The tissue of design is placed on to be placed in sealed shell of tank for a long time, cannot accomplish the real-time observation to tissue.Integrated design causes every time
Pressurized operation include being turned off manually disengaging fluid valve, open compression system；Pressurization, which finishes, every time will also close compression system, beat
Drive liquid valve door into.It is complicated for operation, it is easy to produce human error, liquid is caused to reveal, once there is liquid leakage to will result in dirt
Dye.Integrated design not can be carried out routine and change liquid every other day, is not able to satisfy cell and tissue structrue environmental nutrient condition.Integration
Design, long-term cultivation in incubator will be woven in the group of culture by having resulted in bulky compression chamber, damp and hot ring in incubator
Border is caused each component aging fast, is reduced the stability of equipment, is increased the risk of pollution.Integrated design is so that groups of cells
It knits and is put into reactor, goes out from reactor etc. complicated for operation, and be the high operation of pollution risk.Integrated design, in order to
Reach internal pressurization cabin in the safety condition of high-pressure environment, reactor design weight is very big, is practical operation, carries very tired
Difficulty operates suffering.Integrated design needs uniformly to disappear to entire reactor all parts to reach sterile requirement
Poison, assembling, complicated assembling link need rigorous aseptic, and pollution, which occurs, in a link will lead to the failure of cell tissue, one
Changing design is exactly to pollute amplifier.For this reason, it may be necessary to design a kind of biological culture reactor, it can be used for the biology of high degree of automation
Reaction experiment instrument, and the pollution for carrying out autoreactor can be reduced to greatest extent.
Summary of the invention
The present invention is to provide for a kind of biological culture reaction unit, which can be used for automatic biological reaction experiment instrument,
And the pollution for carrying out autoreactor can be reduced to greatest extent.
To achieve the above object, the technical scheme is that a kind of biological culture reaction unit, including culture cabin plus
Component is pressed, the pressure-applying unit is made of inner ram, top board, lower platen, pressure spring；The inner ram upper surface is fixed with bolt
Top board is connected, inner ram lower end is socketed with lower platen, and inner ram lower part is equipped with boss, and lower platen through-hole upper end is equipped with convex
Ring, lower platen cooperate axially position by bulge loop and inner ram lower boss, move together lower platen can with inner ram upwards；
Pressure spring is socketed with outside inner ram, the both ends of pressure spring are connected with top board, lower platen respectively.Inner ram and lower platen respectively with
Cultivate the gel silica membrane pressure touching connection in cabin, wherein lower platen makes silica gel for compressing gel silica membrane and culture tank body upper surface
It is closely sealed between diaphragm and culture tank body upper surface, inner ram applies setting to the intracorporal liquid of culture tank by gel silica membrane
Pressure.
Further, the pressure-applying unit connects pressurizing device by top board.
Further, the pressurizing device is cylinder, oil cylinder, watches with one of motor or linear motor.
Further, the culture cabin is made of culture tank body, culture cover and gel silica membrane；The culture cover and culture
Tank body is connected through a screw thread, and cultivates and gel silica membrane is arranged between tank body and culture cover, passes through gel silica membrane by culture cover
It is pressed in the preliminary sealing formed between culture cover and culture tank body on culture tank body；When lower platen compresses gel silica membrane and culture
Behind tank body upper surface, pressing force can increase with the pressure of pressurization and be increased, and make tight gel silica membrane and training under spring force
It supports and forms circumferentially uniform close envelope between tank body upper surface.
Further, the culture tank body upper surface be equipped with multiple co-axial grooves rings, when gel silica membrane culture cover and
When lower platen downward pressure acts on, the gel silica membrane is caught in co-axial grooves ring.
Further, stainless billet is additionally provided between the culture cover and culture tank body, culture cover passes through stainless steel
Backing plate compresses connection gel silica membrane, makes gel silica membrane sealing surface stress more evenly, and extend gel silica membrane service life.
The beneficial effects of the present invention are:
The present invention uses pressure-applying unit, is acted on by lower platen and compresses gel silica membrane and culture tank body upper surface, makes silicon
It is closely sealed between glue film and culture tank body upper surface, inner ram applies pressure to culture tank body by gel silica membrane.Wherein, under
Pressing plate compresses gel silica membrane and culture tank body upper surface, and pressing force can increase with the pressure of pressurization oil cylinder and be increased, and in spring
When can ensure that gel silica membrane applies high pressure and pressure maintaining to culture tank body under power effect, tight gel silica membrane and culture tank body upper surface
Between form circumferentially uniform close envelope.
It cultivates cabin and uses stand alone type design, such that routine changes liquid operation every other day, independent culture cabin adds non-
The time of pressure can be with routine culture.Cabin is cultivated by replacement, the service efficiency of biological respinse experiment instrument can be improved.Cultivating cabin can
To carry out 24 hours pressurized operations.
It needs to cultivate cabin under pressure and fills liquid, liquid side is connected with the silica gel sake for cultivating cabin top cover
Touching.So that liquid need to only reach platform, reduces liquid and overflow compression chamber, culture cabin uses double-layer sealing structure, passes through culture
The threaded connection of cover and culture tank body, accomplishes tentatively to seal.Adding for gel silica membrane is awarded by the lower platen in pressure-applying unit
Means of press seals is sealed twice, is not overflowed with reaching the liquid when pressurizeing to compression chamber, accomplishes that safety asepsis operates.
Detailed description of the invention
Fig. 1 is biological culture reaction unit structural upright schematic diagram of the invention；
Fig. 2 is biological culture reaction unit structural front view of the invention；
Fig. 3 is biological culture reaction unit structure sectional view of the invention；
Fig. 4 is biological culture reaction unit and pressurization oil cylinder connection schematic diagram of the invention.
Specific embodiment
The invention will be further described with embodiment with reference to the accompanying drawing.
As shown in Figures 1 to 4, a kind of biological culture reaction unit, including culture cabin 4, pressure-applying unit 5, pressure-applying unit 5 by
Inner ram 5-2, top board 5-3, lower platen 5-4, pressure spring 5-5 composition.The upper surface inner ram 5-2 is fixedly connected with bolt 5-6
The lower end pressing plate 5-3, inner ram 5-2 is socketed with lower platen 5-4, and the lower part inner ram 5-2 is equipped with boss, on lower platen 5-4 through-hole
End is equipped with bulge loop, and lower platen 5-4 cooperates axially position by bulge loop and inner ram 5-2 lower boss, makes lower platen 5-4 can be with interior
Pressure head 5-2 is moved together upwards；Be socketed with pressure spring 5-5 outside inner ram 5-2, the both ends of pressure spring 5-5 respectively with top board 5-3, under
Pressing plate 5-4 is connected.Inner ram 5-2 and lower platen 5-4 is connected with the gel silica membrane 4-3 pressure touching in culture cabin respectively, wherein
Lower platen 5-4 makes on gel silica membrane 4-3 and culture tank body 4-1 for compressing gel silica membrane 4-3 and the culture upper surface tank body 4-1
It is closely sealed between end face, inner ram 5-2 applies the pressure of setting by liquid of the gel silica membrane 4-3 into culture tank body 4-1.
Pressure-applying unit 5 passes through inner ram 5-2 connection pressurizing device.Pressurizing device is cylinder, oil cylinder, watches with motor or straight line
Motor etc..As shown in figure 4, being fixedly connected between top board 5-3 and pressurization oil cylinder 6 by screw thread and nut.
Culture cabin 4 is made of culture tank body 4-1, culture cover 4-2 and gel silica membrane 4-3.Cultivate cover 4-2 and culture tank
Body 4-1 is connected through a screw thread, and cultivates and gel silica membrane 4-3 is arranged between tank body 4-1 and culture cover 4-2, by culture cover 4-
2 are pressed in the preliminary sealing formed between culture cover 4-2 and culture tank body 4-1 on culture tank body 4-1 by gel silica membrane 4-3.
After lower platen 5-4 compresses gel silica membrane 4-3 and the culture upper surface tank body 4-1, pressing force can increase with the pressure of pressurizing device
And increase, and can ensure that gel silica membrane 4-3 to culture tank body 4-1 applies high pressure and when pressure maintaining under spring force, tight silicon
Circumferentially uniform close envelope is formed between glue film 4-3 and the culture upper surface tank body 4-1.
In addition, the culture upper surface tank body 4-1 is equipped with multiple co-axial grooves rings, make gel silica membrane 4-3 in culture cover 4-2
Under the effect of lower platen 5-4 downward pressure, it is caught in co-axial grooves ring, when gel silica membrane 4-3 is by 10Mpa power, makes silica gel
Diaphragm 4-3 can not cause to leak to contract.Stainless billet is additionally provided between culture cover 4-2 and culture tank body 4-1
4-4, culture cover 4-2 compress gel silica membrane 4-3 by stainless billet 4-4, keep gel silica membrane 4-3 sealing surface stress more equal
It is even, and gel silica membrane 4-3 service life can be extended.
When cultivating the pressurization of cabin 1, culture tank body 4-1 fills liquid, the silica gel that liquid side and culture tank body 4-1 are covered
The inner face of thin slice 4-3 is in contact.So that liquid need to only reach tank body 4-1 mouthfuls of culture, reduces liquid and overflow compression chamber.Each
Culture cabin 4 is corresponding with a pressure-applying unit.
In use, first the culture tank body in culture cabin fills liquid, and allows liquid side and cultivate the silica gel of Cap for tin body
Sake is in contact, and screws culture cover, then culture cabin is put into the culture in training area in the cabinet of biological respinse experiment instrument
In the locating slot of cabin, starts biological respinse experiment instrument, move down inner ram and lower platen in pressure-applying unit and be pressed on training
On the gel silica membrane for supporting cabin, then, the controller of biological respinse experiment instrument is by the pressurized component of pressurizing device to gel silica membrane
Press the pressure set, to make to cultivate the application pressure in tank body, and carries out pressure maintaining according to set time.
Claims (6)
1. a kind of biological culture reaction unit, including culture cabin, pressure-applying unit, it is characterised in that: the pressure-applying unit is by internal pressure
Head, top board, lower platen, pressure spring composition；The inner ram upper surface is fixedly connected with top board, the socket of inner ram lower end with bolt
There is lower platen, and inner ram lower part is equipped with boss, lower platen through-hole upper end is equipped with bulge loop, and lower platen passes through under bulge loop and inner ram
Portion's boss cooperates axially position, move together lower platen can with inner ram upwards；It is socketed with pressure spring outside inner ram, the two of pressure spring
End is connected with top board, lower platen respectively.Inner ram and lower platen are connected with the gel silica membrane pressure touching in culture cabin respectively,
Wherein, lower platen makes tight between gel silica membrane and culture tank body upper surface for compressing gel silica membrane and culture tank body upper surface
Sealing, inner ram apply the pressure of setting by gel silica membrane to the intracorporal liquid of culture tank.
2. biological culture reaction unit according to claim 1, it is characterised in that: the pressure-applying unit is connected by top board
Connect pressurizing device.
3. biological culture reaction unit according to claim 2, it is characterised in that: the pressurizing device be cylinder, oil cylinder,
It watches with one of motor or linear motor.
4. biological culture reaction unit according to claim 1, it is characterised in that: the culture cabin is by culture tank body, training
Support cover and gel silica membrane composition；The culture cover is connected through a screw thread with culture tank body, and cultivates tank body and culture cover
Between gel silica membrane is set, by culture cover by gel silica membrane be pressed in culture tank body on is formed culture cover with cultivate tank body it
Between preliminary sealing；When lower platen compress gel silica membrane and culture tank body upper surface after, pressing force with pressurization pressure increase and
Increase, and makes to be formed circumferentially uniform close envelope under spring force between tight gel silica membrane and culture tank body upper surface.
5. biological culture reaction unit according to claim 4, it is characterised in that: the culture tank body upper surface is equipped with
Multiple co-axial grooves rings, when gel silica membrane is when cultivating cover and lower platen downward pressure acts on, the gel silica membrane is caught in together
In axis groove ring.
6. biological culture reaction unit according to claim 4 or 5, it is characterised in that: the culture cover and culture tank
Stainless billet is additionally provided between body, culture cover compresses connection gel silica membrane by stainless billet, seals gel silica membrane
Face stress more evenly, and extends gel silica membrane service life.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811294073.0A CN109370893A (en) | 2018-11-01 | 2018-11-01 | Biological culture reaction unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811294073.0A CN109370893A (en) | 2018-11-01 | 2018-11-01 | Biological culture reaction unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109370893A true CN109370893A (en) | 2019-02-22 |
Family
ID=65396864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811294073.0A Pending CN109370893A (en) | 2018-11-01 | 2018-11-01 | Biological culture reaction unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109370893A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110538003A (en) * | 2019-08-30 | 2019-12-06 | 宁波健世生物科技有限公司 | Heart valve opening and closing detector |
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|---|---|---|---|---|
| CN201817494U (en) * | 2010-08-10 | 2011-05-04 | 中国人民解放军军事医学科学院卫生装备研究所 | Dynamic loading and circular perfusion bioreactor |
| KR20110121924A (en) * | 2010-05-03 | 2011-11-09 | (주)마이크로디지탈 | Pulse apparatus for culture medium and pulse type culture system |
| CN102899250A (en) * | 2012-10-22 | 2013-01-30 | 湖南大学 | Tissue engineering controllable hydraulic bioreactor |
| CN103555579A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Multifunctional bionic tissue culture device |
| CN107858285A (en) * | 2017-12-13 | 2018-03-30 | 刘延群 | Split type bioreactor |
| CN209568108U (en) * | 2018-11-01 | 2019-11-01 | 刘延群 | Biological culture reaction unit |
-
2018
- 2018-11-01 CN CN201811294073.0A patent/CN109370893A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110121924A (en) * | 2010-05-03 | 2011-11-09 | (주)마이크로디지탈 | Pulse apparatus for culture medium and pulse type culture system |
| CN201817494U (en) * | 2010-08-10 | 2011-05-04 | 中国人民解放军军事医学科学院卫生装备研究所 | Dynamic loading and circular perfusion bioreactor |
| CN102899250A (en) * | 2012-10-22 | 2013-01-30 | 湖南大学 | Tissue engineering controllable hydraulic bioreactor |
| CN103555579A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Multifunctional bionic tissue culture device |
| CN107858285A (en) * | 2017-12-13 | 2018-03-30 | 刘延群 | Split type bioreactor |
| CN209568108U (en) * | 2018-11-01 | 2019-11-01 | 刘延群 | Biological culture reaction unit |
Non-Patent Citations (1)
| Title |
|---|
| 陈学忠;侍才洪;李瑞欣;郭勇;李志宏;王亮;张西正;: "新型动态载荷与循环灌流生物反应器系统的设计", 医用生物力学, no. 05, pages 441 - 447 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110538003A (en) * | 2019-08-30 | 2019-12-06 | 宁波健世生物科技有限公司 | Heart valve opening and closing detector |
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