CN109280623A - Biological respinse experiment instrument - Google Patents
Biological respinse experiment instrument Download PDFInfo
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- CN109280623A CN109280623A CN201811294060.3A CN201811294060A CN109280623A CN 109280623 A CN109280623 A CN 109280623A CN 201811294060 A CN201811294060 A CN 201811294060A CN 109280623 A CN109280623 A CN 109280623A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 28
- 239000002828 fuel tank Substances 0.000 claims abstract description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 74
- 239000000499 gel Substances 0.000 claims description 35
- 239000012528 membrane Substances 0.000 claims description 35
- 239000000377 silicon dioxide Substances 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 29
- 238000007789 sealing Methods 0.000 claims description 7
- 239000004973 liquid crystal related substance Substances 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 2
- 239000003086 colorant Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000003921 oil Substances 0.000 description 26
- 210000004027 cell Anatomy 0.000 description 12
- 230000006835 compression Effects 0.000 description 9
- 238000007906 compression Methods 0.000 description 9
- 230000008859 change Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 108010066278 cabin-4 Proteins 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010066057 cabin-1 Proteins 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001459 mortal effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of biological respinse experiment instrument, the control operating space in cabinet below training area is provided with control system, and lower part is equipped with hydraulic system and electric box in cabinet；Multiple culture cabins are placed in training area, each culture cabin connects a pressurization cylinder component, pressurization oil cylinder is mounted on the training area top of cabinet, the oil cylinder that pressurizes connects hydraulic system, hydraulic system connects control system, control system includes PLC controller, operation control panel, circuit board, pressure sensor, and the operation control panel is mounted on the mounting plate before the control operating space of cabinet.Hydraulic system includes fuel tank, high-pressure oil pump, reversal valve, pressure regulator valve, pressure gauge, the pressure sensor is arranged in pressure debugging culture cabin, and PLC controller is connected by signal wire, PLC controller control signal input attended operation control panel, output end is separately connected high-pressure oil pump, reversal valve, pressure regulator valve, and high-pressure oil pump connects pressurization oil cylinder with reversal valve by pressure regulator valve.
Description
Technical field
The present invention relates to a kind of bioreactor, especially a kind of biological respinse experiment instrument belongs to bioreactor technology
Field.
Background technique
Bioreactor is the experiment tool for cultivating tissue of biological cells, is provided daily 1 hour for cell tissue
High pressure culture environment, it is necessary to meet cell and tissue structrue need stringent aseptic condition (once contamination of cells tissue will be by
To irremediable a mortal blow, sterile this is cell culture basis), sufficient nutrient environment (including (various types of cells must for nutrition
Must biotic factor degradation rate it is fast) supply and waste removing), for meet biological tissue needs routinely change every other day
Liquid.
The integrated bioreactor of the existing circulatory system and compression system.Such as patent publication No. CN103589638B,
A kind of pneumatic self-loopa animal cell culture bioreactor and its application method, positioned at big pot bottom magnetic agitation system,
Heating system and control system, big tank upper end are provided with sealing cover, and big tank two sides are provided with the collet connecting with heating system, small
Tank upper end is provided with end socket and its lower end is provided with opening, and end socket and sealing cover are sealedly and fixedly connected by snorkel, snorkel
Upper end is pierced by sealing cover and is connected separately with vacuum pump and air pressure pump, and lower end is pierced by end socket and enters in canister.Such as
Patent publication No. CN102899250B discloses a kind of controlled hydraulic bioreactor for organizational project, the upper end of top cover
Portion is provided with controlled hydraulic system, and the lower part of tank body is provided with circumfusion system；Be provided with flexible diaphragm in tank body, it is flexible every
The cavity that tank body and top cover are formed is separated into culture chamber and pressurizing chamber by film；Using hydraulic pressurization mode and controllable unloading technology,
It can be achieved to realize a variety of stimulus modalities to real-time, the precision control of the key parameters such as Pressure stimulation intensity, frequency, action time
Conversion；Culture solution import and export is introduced in culture systems to realize circumfusion culture.
The circulatory system of the integrated bioreactor of the existing circulatory system and compression system, culture tank body and the bottle that reloads
It is linked by silicone tube, peristaltic pump provides circulation power.It causes and changes liquid and (change liquid within 2 weeks) not in time, culture environment is unfavorable for cell
Tissue cultures.It is high that the circulatory system of multicomponent causes cell tissue pollution rate when changing liquid.The link complexity of various pipes links
Increase pollution risk.The existing circulatory system and combining together of pressure system bioreactor, so that compression chamber is long-term
One group of experiment occupies for a long time, and reactor service efficiency is low.Integrated design is not easy to the observation on Growth to sample, integration
The tissue of design is placed on to be placed in sealed shell of tank for a long time, cannot accomplish the real-time observation to tissue.Integrated design causes every time
Pressurized operation include being turned off manually disengaging fluid valve, open compression system；Pressurization, which finishes, every time will also close compression system, beat
Drive liquid valve door into.It is complicated for operation, it is easy to produce human error, liquid is caused to reveal, once there is liquid leakage to will result in dirt
Dye.Integrated design not can be carried out routine and change liquid every other day, is not able to satisfy cell and tissue structrue environmental nutrient condition.Integration
Design, long-term cultivation in incubator will be woven in the group of culture by having resulted in bulky compression chamber, damp and hot ring in incubator
Border is caused each component aging fast, is reduced the stability of equipment, is increased the risk of pollution.Integrated design is so that groups of cells
It knits and is put into reactor, goes out from reactor etc. complicated for operation, and be the high operation of pollution risk.Integrated design, in order to
Reach internal pressurization cabin in the safety condition of high-pressure environment, reactor design weight is very big, is practical operation, carries very tired
Difficulty operates suffering.Integrated design needs uniformly to disappear to entire reactor all parts to reach sterile requirement
Poison, assembling, complicated assembling link need rigorous aseptic, and pollution, which occurs, in a link will lead to the failure of cell tissue, one
Changing design is exactly to pollute amplifier.Therefore, it is necessary to design a kind of high degree of automation, can simultaneously to the progress of multiple culture tank bodies not
With desired experiment, and the biological respinse experiment instrument for the pollution for carrying out autoreactor can be reduced to greatest extent.
Summary of the invention
The present invention is to provide for a kind of biological respinse experiment instrument, the biological respinse experiment instrument have high degree of automation, can
The experiment of different requirements is carried out to multiple culture tank bodies simultaneously, and the pollution for carrying out autoreactor can be reduced to greatest extent.
To achieve the above object, the technical scheme is that a kind of biological respinse experiment instrument, including cabinet, control system
System, hydraulic system, electric box, the cabinet top are equipped with training area, the control operation in cabinet below training area
Area is provided with control system, and lower part is equipped with hydraulic system and electric box in cabinet；Multiple culture cabins are placed in training area,
In one be pressure debugging culture cabin, each culture cabin connects a pressurization cylinder component, and the pressurization in pressurization cylinder component is oily
Cylinder is mounted on the training area top of cabinet, and pressurization oil cylinder connects hydraulic system, and hydraulic system connects control system, control system
System connects electric box with hydraulic system, and the control system includes PLC controller, operation control panel, circuit board, pressure sensing
Device, the operation control panel are mounted on the mounting plate before the control operating space of cabinet.Hydraulic system includes fuel tank, high pressure
Oil pump, reversal valve, pressure regulator valve, pressure gauge, the pressure sensor are arranged in pressure debugging culture cabin, and are connected by signal wire
PLC controller, PLC controller control signal input attended operation control panel are connect, output end is separately connected high-pressure oil pump, changes
To valve, pressure regulator valve, high-pressure oil pump connects pressurization oil cylinder with reversal valve by pressure regulator valve.
Further, training area bottom is equipped with culture cabin locating slot, and cultivates the corresponding pressurization oil cylinder of cabin locating slot
Component.
Further, watch window is equipped in the cabinet before training area, convenient for being seen in real time to cell tissue
It examines.
Further, the operation control panel is 10 cun of color liquid crystal touch screens, and operating on control panel, there is starting to press
Each culture tank can be set separately by pressure adjustment button in button, stop button, pressure adjustment button, time adjustment button
It is upper pressurization oil cylinder independent control pressure value: 0-10Mpa, and by time adjustment button adjust pressurization oil cylinder pressure maintaining when
Between: 0-99999s.
Further, the pressurization cylinder component includes pressurization oil cylinder, inner ram, top board, lower platen, pressure spring；It is described to add
The piston rod of compressing cylinder is fixedly connected with top board, and top board is fixedly connected with inner ram with bolt, and inner ram lower end is socketed with pushing
Plate, inner ram are socketed with pressure spring outside, and the both ends of pressure spring are connected with top board, lower platen respectively；Inner ram and lower platen point
It is not connected with the gel silica membrane pressure touching in culture cabin, wherein lower platen makes for compressing gel silica membrane and culture tank body upper surface
It is closely sealed between gel silica membrane and culture tank body upper surface, inner ram applies pressure to culture tank body by gel silica membrane.
Further, the culture cabin is made of culture tank body, culture cover and gel silica membrane, the culture cover and culture
Tank body is connected through a screw thread, and cultivates and gel silica membrane is arranged between tank body and culture cover, passes through gel silica membrane by culture cover
It is pressed in the preliminary sealing formed between culture cover and culture tank body on culture tank body.
Further, in use, first the culture tank body in culture cabin fills liquid, and make liquid side and cultivate the silicon of Cap for tin body
Glue sake is in contact, and screws culture cover, then culture cabin is put into the culture cabin locating slot in training area in cabinet, starts
Hydraulic system makes inner ram and lower platen in pressurization cylinder component move down and be pressed on the gel silica membrane in culture cabin,
Then, the pressure that PLC controller sets gel silica membrane pressure by HYDRAULIC CONTROL SYSTEM pressurization cylinder component, to make to cultivate
Liquid in tank body applies pressure, and carries out pressure maintaining according to set time.
The beneficial effects of the present invention are:
Biological respinse experiment instrument of the invention carries out pressurization and pressure maintaining to culture cabin using pressurization cylinder component, and uses
The pressure that PLC controller sets gel silica membrane pressure by HYDRAULIC CONTROL SYSTEM pressurization cylinder component, to make to cultivate tank body
In liquid apply pressure, and carry out pressure maintaining according to set time, the size to culture cabin applied force can be precisely controlled, continue
It is time, safer, efficiently, the experiment of the biological respinse suitable for all kinds of culture cabins, and have with high degree of automation, operation
The features such as facilitating.
In addition, being acted on by lower platen using pressurization cylinder component and compressing gel silica membrane and culture tank body upper surface, made
It is closely sealed between gel silica membrane and culture tank body upper surface, inner ram applies pressure to culture tank body by gel silica membrane.Wherein,
Lower platen compresses gel silica membrane and culture tank body upper surface, and pressing force can increase with the pressure of pressurization oil cylinder and be increased, and in bullet
When can ensure that gel silica membrane applies high pressure and pressure maintaining to culture tank body under spring force effect, tight gel silica membrane and culture tank body upper end
Close envelope between face.
It cultivates cabin and uses stand alone type design, such that routine changes liquid operation every other day, independent culture cabin adds non-
The time of pressure can be with routine culture.Cabin is cultivated by replacement, the service efficiency of biological respinse experiment instrument can be improved.Cultivating cabin can
To carry out 24 hours pressurized operations.Cell tissue is carried out in real time by the way that the watch window in cabinet before training area is convenient
Observation, understands cell growth status in time.The pressurization lid in culture cabin can be can also be used as disposable sterilized with autoclave sterilization
Consumptive material, disinfection is convenient, comprehensive guarantee gnotobasis.
It needs to cultivate cabin under pressure and fills liquid, liquid side is connected with the silica gel sake for cultivating cabin top cover
Touching.So that liquid need to only reach platform, reduces liquid and overflow compression chamber, culture cabin uses double-layer sealing structure, passes through culture
The threaded connection of cover and culture tank body, accomplishes tentatively to seal.Gel silica membrane is awarded by the lower platen in pressurization cylinder component
Pressurization, sealed, do not overflowed with reaching the liquid when pressurizeing to compression chamber twice, accomplish safety asepsis operate.
Detailed description of the invention
Fig. 1 is biological respinse experiment instrument structural upright schematic diagram of the invention；
Fig. 2 is biological respinse experiment instrument structural front view of the invention；
Fig. 3 is biological respinse experiment instrument structure sectional view of the invention；
Fig. 4 is biological respinse experiment instrument control system functional block diagram of the invention；
Fig. 5 is culture cabin and pressurization cylinder component (in addition to the oil cylinder that pressurizes) structural upright schematic diagram；
Fig. 6 is culture cabin and pressurization cylinder component (in addition to the oil cylinder that pressurizes) structure sectional view；
Fig. 7 is the workflow block diagram of biological respinse experiment instrument of the invention.
Specific embodiment
The invention will be further described with embodiment with reference to the accompanying drawing.
As shown in Figures 1 to 6, a kind of biological respinse experiment instrument, including it is cabinet 1, control system 2, hydraulic system 3, electrical
Case.1 top of cabinet is equipped with training area, and the control operating space in cabinet below training area is provided with control system 2, machine
Lower part is equipped with hydraulic system 3 and electric box in case 1.Multiple culture cabins 4 can be placed in training area, wherein matching a pressure tune
Examination culture cabin；Training area bottom is equipped with culture cabin locating slot, and each culture cabin 4 connects a pressurization cylinder component 5, pressurization
Pressurization oil cylinder 5-1 in cylinder component 5 is mounted on the training area top of cabinet 1, oil cylinder 5-1 connection hydraulic system 3 of pressurizeing,
Hydraulic system 3 connects control system 2, and control system 2 and hydraulic system 3 connect electric box, supply electricity to control system 2 by electric box
With hydraulic system 3.
Control system 2 includes PLC controller 2-1, operation control panel 2-2, circuit board, pressure sensor etc..Operation control
Panel 2-1 processed is mounted on the mounting plate before the control operating space of cabinet 1.Hydraulic system 3 includes fuel tank, high-pressure oil pump, changes
To valve, pressure regulator valve, pressure gauge etc..Pressure sensor is arranged in pressure debugging culture cabin, and connects PLC control by signal wire
Device 2-2, PLC controller 2-2 control signal input attended operation control panel 2-1, output end are separately connected high-pressure oil pump, change
To valve, pressure regulator valve, high-pressure oil pump connects pressurization oil cylinder 5-1 with reversal valve by pressure regulator valve.Operation control panel 2-1 is 10 cun of coloured silks
Dichroic liquid crystal touch screen.On operation control panel 2-1 there is start button, stop button, pressure adjustment button, time adjustment to press
Pressure value (the 0- of the independent control for the oil cylinder that pressurizes in each culture tank can be set separately by pressure adjustment button for button
10Mpa), and by time adjustment button the dwell time (0-99999s) of pressurization oil cylinder is adjusted.
The cylinder component 5 that pressurizes includes pressurization oil cylinder 5-1, inner ram 5-2, top board 5-3, lower platen 5-4, pressure spring 5-5.Add
The piston rod of compressing cylinder 5-1 is fixedly connected with top board 5-3, and top board 5-3 is fixedly connected with inner ram 5-2, inner ram with bolt 5-6
The lower end 5-2 is socketed with lower platen 5-4, is socketed with pressure spring 5-5 outside inner ram 5-2, the both ends of pressure spring 5-5 respectively with top board 5-3,
Lower platen 5-4 is connected.Inner ram 5-2 and lower platen 5-4 is connected with the gel silica membrane 4-3 pressure touching in culture cabin respectively,
In, lower platen 5-4 makes gel silica membrane 4-3 and culture tank body 4-1 for compressing gel silica membrane 4-3 and the culture upper surface tank body 4-1
It is closely sealed between upper surface, inner ram 5-2 applies pressure to culture tank body 4-1 by gel silica membrane 4-3.
Culture cabin 4 is made of culture tank body 4-1, culture cover 4-2 and gel silica membrane 4-3.Cultivate cover 4-2 and culture tank
Body 4-1 is connected through a screw thread, and cultivates and gel silica membrane 4-3 is arranged between tank body 4-1 and culture cover 4-2, by culture cover 4-2
The preliminary sealing formed between culture cover 4-2 and culture tank body 4-1 on culture tank body 4-1 is pressed in by gel silica membrane 4-3.
When cultivating the pressurization of cabin 1, culture tank body 4-1 fills liquid, the silica gel that liquid side and culture tank body 4-1 are covered
The inner face of thin slice 4-3 is in contact.So that liquid need to only reach tank body 4-1 mouthfuls of culture, reduces liquid and overflow compression chamber.
In use, first the culture tank body in culture cabin fills liquid, and allows liquid side and cultivate the silica gel of Cap for tin body
Sake is in contact, and screws culture cover, then culture cabin is put into the culture cabin locating slot in training area in cabinet, starts liquid
Pressure system makes inner ram and lower platen in pressurization cylinder component move down and be pressed on the gel silica membrane in culture cabin, so
Afterwards, the pressure that PLC controller sets gel silica membrane pressure by HYDRAULIC CONTROL SYSTEM pressurization cylinder component, to make culture tank
Liquid in body applies pressure, and carries out pressure maintaining according to set time.
Concrete application embodiment:
1) it is placed respectively in training area in cabinet there are four cabin is cultivated, pressurization oil cylinder group is connected on each culture cabin
Part, pressurize cylinder component in pressurization oil cylinder be can independent control the rectangular hydraulic cylinder of 50*50mm: cylinder diameter 50mm, bar diameter
30mm, stroke dimension 50mm, the movement speed of maximum pressure 21Mpa (system pressure) pressurization oil cylinder: 10-15mm/s.
2) control system uses PLC controller and 10 cun of color liquid crystal touch screens, can set four independent controls respectively
Pressure value (0-10Mpa), the dwell time (0-99999s), the dwell time, unit: second；Setting range: 1-99999s.And it sets
There are start button, stop button.
3) it is red that power switch, power supply indicator are set, hydraulic system starting, hydraulic system start indicator lamp on cabinet
(green), hydraulic closure system indicator light (yellow).
4) workflow: as shown in fig. 7, setting pressure (sectional sets 1-10 pressure value), dwell time (sectional
Set 1-10 time value), start button-oil cylinder starts movement to setup pressure value -- and keep pressure ± 5%-- to reach last
Setting time value-oil cylinder pressure release returns to origin (repeatable circulation setting value).
Claims (7)
1. a kind of biological respinse experiment instrument, including cabinet, control system, hydraulic system, electric box, it is characterised in that: the machine
Upper box part is equipped with training area, and the control operating space in cabinet below training area is provided with control system, under cabinet is interior
Portion is equipped with hydraulic system and electric box；Multiple culture cabins are placed in training area, one of them is pressure debugging culture cabin, often
A culture cabin connects a pressurization cylinder component, and the pressurization oil cylinder in the cylinder component that pressurizes is mounted in the training area of cabinet
Portion, pressurization oil cylinder connect hydraulic system, and the hydraulic system connects control system, and the control system connects electricity with hydraulic system
Gas tank, the control system include PLC controller, operation control panel, circuit board, pressure sensor, the operation control plane
Plate is mounted on the mounting plate before the control operating space of cabinet；The hydraulic system include fuel tank, high-pressure oil pump, reversal valve,
Pressure regulator valve, pressure gauge, the pressure sensor are arranged in pressure debugging culture cabin, and connect PLC controller by signal wire,
PLC controller control signal input attended operation control panel, output end are separately connected high-pressure oil pump, reversal valve, pressure regulator valve,
High-pressure oil pump connects pressurization oil cylinder with reversal valve by pressure regulator valve.
2. biological respinse experiment instrument according to claim 1, it is characterised in that: training area bottom is equipped with culture
Cabin locating slot, and cultivate the corresponding pressurization cylinder component of cabin locating slot.
3. biological respinse experiment instrument according to claim 1, it is characterised in that: set before training area in the cabinet
There is watch window, convenient for being observed in real time cell tissue.
4. biological respinse experiment instrument according to claim 1, it is characterised in that: the operation control panel is 10 cun of colours
Liquid crystal touch screen, operating has start button, stop button, pressure adjustment button, time adjustment button on control panel, passes through
The pressure value of the independent control for the oil cylinder that pressurizes in each culture tank: 0-10Mpa, Yi Jitong can be set separately in pressure adjustment button
Spend the dwell time that time regulation button adjusts pressurization oil cylinder: 0-99999s.
5. biological respinse experiment instrument according to claim 1, it is characterised in that: the pressurization cylinder component includes pressurization oil
Cylinder, inner ram, top board, lower platen, pressure spring；The piston rod of the pressurization oil cylinder is fixedly connected with top board, top board bolt
Be fixedly connected with inner ram, inner ram lower end is socketed with lower platen, be socketed with pressure spring outside inner ram, the both ends of pressure spring respectively with upper pressure
Plate, lower platen connect；The inner ram and lower platen are connected with the gel silica membrane pressure touching in culture cabin respectively, wherein under
Pressing plate is used to compress gel silica membrane and culture tank body upper surface, makes closely to seal between gel silica membrane and culture tank body upper surface, interior
Pressure head applies pressure to culture tank body by gel silica membrane.
6. biological respinse experiment instrument according to claim 1, it is characterised in that: the culture cabin is by culture tank body, culture
Cover and gel silica membrane composition, the culture cover and culture tank body be connected through a screw thread, and cultivate tank body with cultivate cover it
Between gel silica membrane is set, by culture cover by gel silica membrane be pressed in culture tank body on is formed culture cover and cultivate tank body between
Preliminary sealing.
7. -6 any biological respinse experiment instrument according to claim 1, it is characterised in that: in use, first in culture cabin
Culture tank body fills liquid, and liquid side is made to be in contact with the silica gel sake for cultivating Cap for tin body, screws culture cover, then will culture
Cabin is put into the culture cabin locating slot in training area in cabinet, starts hydraulic system, makes the inner ram in pressurization cylinder component
And lower platen is moved down and is pressed on the gel silica membrane in culture cabin, then, PLC controller is pressurizeed by HYDRAULIC CONTROL SYSTEM
Cylinder component to gel silica membrane pressure setting pressure, thus make cultivate tank body in liquid apply pressure, and by setting when
Between carry out pressure maintaining.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811294060.3A CN109280623A (en) | 2018-11-01 | 2018-11-01 | Biological respinse experiment instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811294060.3A CN109280623A (en) | 2018-11-01 | 2018-11-01 | Biological respinse experiment instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109280623A true CN109280623A (en) | 2019-01-29 |
Family
ID=65174771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811294060.3A Pending CN109280623A (en) | 2018-11-01 | 2018-11-01 | Biological respinse experiment instrument |
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| CN (1) | CN109280623A (en) |
Citations (8)
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| CN1800339A (en) * | 2005-09-29 | 2006-07-12 | 江苏大学 | Integral device for inoculating and culturing for edible mushroom liquid strain |
| CN201151719Y (en) * | 2007-12-24 | 2008-11-19 | 金灵桥 | High-pressure cell crusher |
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| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
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| CN107858285A (en) * | 2017-12-13 | 2018-03-30 | 刘延群 | Split type bioreactor |
| CN209227000U (en) * | 2018-11-01 | 2019-08-09 | 刘延群 | Biological respinse experiment instrument |
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| CN1800339A (en) * | 2005-09-29 | 2006-07-12 | 江苏大学 | Integral device for inoculating and culturing for edible mushroom liquid strain |
| CN201151719Y (en) * | 2007-12-24 | 2008-11-19 | 金灵桥 | High-pressure cell crusher |
| CN102899250A (en) * | 2012-10-22 | 2013-01-30 | 湖南大学 | Tissue engineering controllable hydraulic bioreactor |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN205443299U (en) * | 2015-12-23 | 2016-08-10 | 天津滨通生物科技有限公司 | Automatic extrusion control system of stem cell separator |
| CN107858285A (en) * | 2017-12-13 | 2018-03-30 | 刘延群 | Split type bioreactor |
| CN209227000U (en) * | 2018-11-01 | 2019-08-09 | 刘延群 | Biological respinse experiment instrument |
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