CN217351377U

CN217351377U - Common general application of sample low temperature box of molecular biology experiment

Info

Publication number: CN217351377U
Application number: CN202123113434.7U
Authority: CN
Inventors: 张松梅; 曹启龙; 梁少帅; 董永丽; 郝利利; 乔羽
Original assignee: Qingdao Haier Biotechnology Co ltd
Current assignee: Qingdao Haier Biotechnology Co ltd
Priority date: 2021-12-10
Filing date: 2021-12-10
Publication date: 2022-09-02
Anticipated expiration: 2031-12-10

Abstract

The utility model relates to a molecular biology experiment equipment technical field specifically is a general application of sample low-temperature box of common molecular biology experiment, including lower box, upper cover, low temperature module and refrigerator, lower box is connected with the upper cover, the upper cover forms the box with lower box is sealed, the inside of lower box is provided with the low temperature module, the low temperature module can be dismantled with lower box and be connected, the low temperature module includes unit application of sample module, keep apart through the insulating layer between the unit application of sample module, a plurality of unit application of sample modules are used for placing centrifuging tube, PCR pipe or cell culture board, singly unit application of sample module inside be provided with the refrigeration space, the refrigerator provides the cold source for the refrigeration space, the refrigerator passes through mains operated, the refrigerator can be dismantled with unit application of sample module as required and be connected, low temperature duration is long to applicable multiple experiment.

Description

Common general application of sample low temperature box of molecular biology experiment

Technical Field

The utility model belongs to the technical field of molecular biology experiment equipment, specifically say, the utility model relates to a general application of sample cryostat of common molecular biology experiment.

Background

In biological tests in biological laboratories, centrifuge tubes, freezing centrifuge tubes, PCR centrifuge tubes, and the like are commonly used to collect blood samples and other biological samples, and related works such as centrifugation, nucleic acid extraction, electrophoresis, protein determination, nucleic acid detection, and the like are performed, and all the works are completed in a low-temperature environment of 2-4 ℃. However, the current bracket for holding the sampling device generally has no low-temperature device, and the bracket needs to be repeatedly placed in a refrigerator or directly placed on an ice block to meet the requirement of the working environment for storing the sample at low temperature, so that the operation is troublesome and unsafe.

At present, ice is made by an ice making machine and stored in a foam box or an ice bucket to create a low-temperature environment or a single-function centrifuge tube frame made of a phase-change cold storage material is used.

SUMMERY OF THE UTILITY MODEL

The utility model aims at the problem that above-mentioned exists, the utility model provides a general application of sample application low-temperature box of common molecular biology experiment, but wide application in the conventional low temperature (4 ℃ -10 ℃) environmental operation experiment that needs of molecular biology laboratory, solved the PCR application of sample and joined in marriage liquid, the liquid is joined in marriage in the fluorescence ration PCR application of sample, the liquid is joined in marriage in the ELISA application of sample, WB application of sample is joined in marriage in experimental operation such as liquid and is deposited in the trash ice and easily emptys, pollute and use only the single or a small amount of centrifuging tube size of adaptation and join in marriage liquid aluminium system module and be not suitable for multiple type experiment operation problem.

In order to solve the above problem, the utility model provides a following technical scheme: the utility model provides a general application of sample cryostat of common molecular biology experiment, includes box, upper cover, low temperature module and refrigerator down, lower box be connected with the upper cover, upper cover and the sealed box that forms of lower box, the inside of lower box is provided with the low temperature module, the low temperature module can dismantle with lower box and be connected, the low temperature module include unit application of sample module, unit application of sample module between keep apart through the insulating layer, a plurality of unit application of sample modules are used for placing centrifuging tube, PCR pipe or cell culture board, singly unit application of sample module inside be provided with the refrigeration space, the refrigerator provides the cold source for the refrigeration space, the refrigerator passes through mains operated, the refrigerator can dismantle with unit application of sample module as required and be connected.

Furthermore, the low-temperature module is made of aluminum alloy.

Furthermore, the upper cover is hinged with the lower box body, a handle is arranged at the top of the upper cover, and the upper cover and the lower box body are fixed through a snap-fastener door lock.

Furthermore, a storage box is further arranged at the top of the upper cover, and a storage box door is further arranged on the storage box.

Furthermore, one side of the lower part of the lower box body is provided with an installation window communicated with the interior of the lower box body for a human hand to stretch into the interior space of the lower box body for installing the refrigerator, the lower box body is also provided with a magnetic suction door for sealing the installation window, and one side or multiple sides of the bottom of the lower box body are provided with heat dissipation holes for heat dissipation of a heat dissipation fan.

Furthermore, the bottom of lower box be provided with the rubber layer, the bottom rubber layer can increase frictional force and make the low-temperature box steadily place on the laboratory bench, can not lead to experimental operator's trace application of sample mistake or the extravagant experimental accident of reagent because of the low-temperature box removes.

The refrigerator comprises a heat radiation fan, a heat radiation fin, a semiconductor refrigeration piece and a cold guide piece, wherein the semiconductor refrigeration piece is electrically connected with a power supply, the cold radiation surface of the semiconductor refrigeration piece is attached to one surface of the cold guide piece, and the other surface of the cold guide piece is refrigerated towards the internal refrigeration space of the unit sample adding module; the heat dissipation surface of the semiconductor refrigeration piece is attached to one surface of the heat dissipation piece, the other surface of the heat dissipation piece is attached to the air inlet surface of the heat dissipation fan, and the heat dissipation fan is electrically connected with the power supply.

Further, the periphery of cold conducting sheet be provided with and lead a cold section of thick bamboo, the bottom of unit application of sample module be provided with the cold mouth that advances of inside refrigeration space intercommunication, advance cold mouth and lead a cold section of thick bamboo and peg graft, the top of leading a cold section of thick bamboo be provided with the collar, the collar on be provided with magnet, magnet adsorbs whole refrigerator in the bottom of unit application of sample module, the bottom of unit application of sample module is provided with the adsorbable metal zone of magnet.

Further, unit application of sample module include the unit refrigeration case, the unit refrigeration case divide into the centrifuging tube and deposits district and cell culture board and deposit the district, the top of the unit refrigeration case in centrifuging tube storage area is the roof, the inside of unit refrigeration case is provided with deflector and bottom plate, the roof, deflector and bottom plate are from last interval arrangement downwards in proper order, all run through on roof and the deflector and have vertical unanimous centrifuging tube to place the hole, the deflector of different centrifuging tube storage areas and the high nonconformity of bottom plate. The top of the unit refrigeration box of the cell culture plate storage area is a top plate which is a flat plate.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses a low temperature module combines with the refrigerator for the low temperature time of unit application of sample module prolongs greatly, and the low temperature module adopts the aluminum alloy material, and aluminum alloy material has that density is little, corrosion resistance is good, the aspect advantage such as specific strength height and low cost, and the refrigerator further prolongs the low temperature time when low temperature module temperature will become high, can keep joining in marriage liquid in-process low temperature environment and furthest preserves active ingredient in the reaction liquid, prevents active ingredient degradation, degeneration and pollution scheduling problem.

2. This low-temperature box fungible tradition ice bucket trash ice, simple and convenient, clean environmental protection, this unit application of sample module can use repeatedly, and the metal material can not be ageing, and is clean convenient, does not rust, and is lossless, can use for a long time, and special space metal material energy storage is quick, unlimited circulation freezes deposits, does not age and does not fade, heat conduction sensitivity, temperature homogeneity, especially provide better care to the accurate precious sensitive enzyme reagent of temperature homogeneity requirement.

3. A plurality of unit application of sample modules can be suitable for the liquid operation is joined in marriage to the low temperature of multiple molecular biological assay, can satisfy the centrifuging tube of depositing multiple size aperture.

4. The refrigerator provides the cold source for in the single unit application of sample module alone for refrigeration efficiency in the unit application of sample module is higher, separates with the insulating layer between the unit application of sample module, reduces the route of heat transfer, reduces dispersing of cold source.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic view of the usage state of the present invention;

FIG. 2 is a schematic view of the overall structure of the present invention;

fig. 3 is a right side view of the present invention;

fig. 4 is a cross-sectional view taken along a-a of fig. 3 according to the present invention;

FIG. 5 is a top view of the present invention with the upper cover removed;

fig. 6 is a cross-sectional view taken along line D-D of fig. 5 in accordance with the present invention;

fig. 7 is a schematic view of the refrigerator of the present invention.

Reference numerals are as follows: 1. a lower box body; 2. an upper cover; 3. a low temperature module; 4. a magnetic door; 5. installing a window; 6. a base; 7. a handle; 8. a storage box; 9. a snap-fastener door lock; 10. a refrigerator; 31. a cold inlet; 32. a unit sample adding module; 33. a thermal insulation layer; 34. a unit refrigeration box; 35. a guide plate; 36. a base plate; 37. a top plate; 38. placing a hole in a centrifuge tube; 101. a mounting ring; 102. a cold conducting sheet; 103. a semiconductor refrigeration sheet; 104. a cold conducting cylinder; 105. a heat sink; 106. a heat dissipation fan.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected or detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.

As shown in figures 1-7

Example one

The utility model provides a following technical scheme: a common sample-adding low-temperature box for molecular biology experiments comprises a lower box body 1, an upper cover 2, a low-temperature module 3 and a refrigerator 10, wherein the lower box body 1 is connected with the upper cover 2, the upper cover 2 and the lower box body 1 are sealed to form a box body, the low-temperature module 3 is arranged in the lower box body 1, the low-temperature module 3 is detachably connected with the lower box body 1, the low-temperature module 3 comprises unit sample-adding modules 32, the unit sample-adding modules 32 are isolated by heat insulation layers, a plurality of unit sample-adding modules 32 are used for placing centrifuge tubes, PCR tubes or cell culture plates, a single refrigerating space is arranged in each unit sample-adding module 32, each unit sample-adding module 32 comprises a unit refrigerating box 34, the lower part in each unit refrigerating box 34 is a refrigerating space, the upper part in each unit refrigerating box 34 is used for placing the space in each centrifuge tube, each unit box 34 is divided into a centrifuge tube storage area and a cell culture plate storage area, the top of the unit refrigeration case 34 in the centrifuging tube parking area is roof 37, and the inside of unit refrigeration case 34 is provided with deflector 35 and bottom plate 36, and roof 37, deflector 35 and bottom plate 36 are from the top down interval arrangement in proper order, all run through the vertical unanimous centrifuging tube on roof 37 and the deflector 35 and place the hole, and the height of the deflector 35 and the bottom plate 36 in different centrifuging tube parking areas is inconsistent. The top of the unit refrigeration cassette 34 of the cell culture plate storage area is a top plate 37, and the top plate 37 is a flat plate. The refrigerator 10 provides a cold source for a refrigerating space, the refrigerator 10 is powered by a power supply, the refrigerator 10 is detachably connected with the unit sample adding module 32 as required, the refrigerator 10 comprises a radiating fan 106, a radiating fin 105, a semiconductor refrigerating sheet 103 and a cold guide sheet 102, the radiating fin 105 and the cold guide sheet 102 are both in a grid type, the semiconductor refrigerating sheet 103 is electrically connected with the power supply, a cold radiating surface of the semiconductor refrigerating sheet 103 is attached and connected with one surface of the cold guide sheet 102, and the other surface of the cold guide sheet 102 is used for refrigerating the internal refrigerating space of the unit sample adding module 32; semiconductor refrigeration piece 103's cooling surface and the laminating of fin 105 one side, the another side of fin 105 and radiator fan 106's air inlet face laminating, radiator fan 106 be connected with the power electricity, the periphery of leading cold piece 102 be provided with and lead cold section of thick bamboo 104, unit application of sample module 32's bottom be provided with the cold mouth 31 of advancing of inside refrigeration space intercommunication, advance cold mouth 31 and lead cold section of thick bamboo 104 and peg graft, the top of leading cold section of thick bamboo 104 be provided with collar 101, collar 101 on be provided with magnet, magnet adsorbs whole refrigerator 10 in unit application of sample module 32's bottom, the bottom of unit application of sample module 32 is provided with the adsorbable metal area of magnet.

Furthermore, the low-temperature module 3 is made of aluminum alloy. The aluminum alloy material is widely applied to the industrial fields of aviation, aerospace, automobiles and the like, the aluminum alloy has good heat-conducting property, and a module treated by an anodic oxidation technology not only retains the rapid heat-conducting property, but also has enough corrosion resistance. The sample adding module is frozen in a refrigerator at minus 80 ℃ and minus 20 ℃ for 30 minutes, then taken out and recovered to 4 ℃ in about 60 minutes, and recovered to room temperature in about 120 minutes.

The upper cover 2 is hinged with the lower box body 1, a handle 7 is arranged at the top of the upper cover 2, and the upper cover 2 and the lower box body 1 are fixed through a snap-fastener door lock 9. The top of the upper cover 2 is also provided with a storage box 8 for placing experimental articles, and the storage box 8 is also provided with a storage box door. One side of the lower part of the lower box body 1 is provided with an installation window 5 communicated with the interior of the lower box body 1 for a human hand to extend into the interior space of the lower box body 1 for installing a refrigerator 10, and the lower box body 1 is also provided with a magnetic door 4 for sealing the installation window 5. One or more surfaces of the bottom of the lower case 1 are provided with heat dissipation holes for dissipating heat of the heat dissipation fan 106. The bottom of lower box 1 is provided with the rubber layer, and the bottom rubber layer can increase frictional force and make the low-temperature box steadily place on the laboratory bench, can not lead to experimental operator's trace application of sample mistake or the extravagant experimental accident of reagent because of the low-temperature box removes.

The use process comprises the following steps: when a molecular biology experiment needs low-temperature solution preparation, the commonly performed experiment is one or more of PCR (polymerase chain reaction) sample adding and solution preparation, fluorescence quantitative PCR sample adding and solution preparation, ELISA (enzyme-linked immunosorbent assay) sample adding and solution preparation, WB (WB) sample adding and solution preparation, and the like, firstly, a low-temperature module 3 is placed in a refrigerator for precooling, and then taken out and placed in a lower box body 1, the experiment is that a centrifuge tube or a cell culture plate needing low-temperature solution preparation is placed in a unit sample adding module 32 which needs to be placed, as shown in FIG. 5, a centrifuge tube storage area of 1.5ml, a centrifuge tube storage area of 2ml and a centrifuge tube storage area of 0.6ml are sequentially arranged at the upper left corner from top to bottom, and a centrifuge tube storage area of 15ml and a centrifuge tube storage area of 50ml are sequentially arranged at the upper right corner from left to right; the lower left corner is a 0.2ml PCR tube (or 8 rows of PCR tubes, PCR plates) storage area; the lower right corner is a cell culture plate storage area, the height of a guide plate 35 and a bottom plate 36 in each centrifuge tube storage area is different, and for example, the hole site diameter of a 1.5ml centrifuge tube storage area is 11mm by 24.81 mm; the diameter and depth of the hole site in the storage area of the 2ml centrifuge tube are 11mm and 28.86 mm; the diameter and depth of the hole site in the storage area of a 0.6ml centrifuge tube are 6mm and 20 mm; the diameter and depth of the hole site of the 15ml centrifuge tube storage area are 17mm and 53 mm; the diameter and depth of the hole site in the storage area of a 50ml centrifuge tube are 28.45mm and 50 mm; the hole site diameter and depth of the 0.2ml PCR tube storage area are 5.72mm and 13 mm; the length and width of the cell culture plate reservoir was 128mm and 85.2 mm.

After placing the centrifuge tube or the cell culture plate, if the cell culture plate needs to be transferred, the upper cover 2 is closed, the cell culture plate is fixed by the snap door lock 9, the cell culture plate is lifted and moved to a needed storage area through the handle 7, after the temperature of the low-temperature module 3 rises, the refrigerator 10 is additionally arranged at the bottom of a certain unit sample adding module 32 as required, the refrigerator is adsorbed at the bottom of the unit sample adding module 32 through the cooperation of the cold inlet 31 and the cold guide cylinder 104 and the magnet of the mounting ring 101, the power supply of the semiconductor refrigerating sheet 103 is turned on, refrigeration is carried out, and the low-temperature time is prolonged.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a general application of sample application low temperature box of common molecular biology experiment which characterized in that: comprises a lower box body (1), an upper cover (2), a low-temperature module (3) and a refrigerator (10), the lower box body (1) is connected with the upper cover (2), the upper cover (2) and the lower box body (1) form a box body in a sealing way, the lower box body (1) is internally provided with a low-temperature module (3), the low-temperature module (3) is detachably connected with the lower box body (1), the low-temperature module (3) comprises a unit sample adding module (32), the unit sample adding modules (32) are isolated by a heat insulation layer, a plurality of unit sample adding modules (32) are used for placing centrifuge tubes, PCR tubes or cell culture plates, a refrigerating space is arranged in a single unit sample adding module (32), the refrigerator (10) provides a cold source for the refrigerating space, the refrigerator (10) is powered by a power supply, the refrigerator (10) is detachably connected with the unit sample adding module (32) according to requirements.

2. The universal sample-adding low-temperature box for common molecular biology experiments according to claim 1, which is characterized in that: the low-temperature module (3) is made of aluminum alloy.

3. The universal sample-adding low-temperature box for common molecular biology experiments according to claim 2, which is characterized in that: the upper cover (2) is hinged with the lower box body (1), a handle (7) is arranged at the top of the upper cover (2), and the upper cover (2) is fixed with the lower box body (1) through a snap door lock (9).

4. The universal sample-adding low-temperature box for common molecular biology experiments according to claim 3, wherein: the top of the upper cover (2) is also provided with a storage box (8), and a storage box door is also arranged on the storage box (8).

5. The universal sample-adding low-temperature box for common molecular biology experiments according to claim 4, wherein: the novel heat dissipation box is characterized in that a mounting window (5) communicated with the interior of the lower box body (1) is formed in one surface of the lower portion of the lower box body (1), a magnetic door (4) used for sealing the mounting window (5) is further arranged on the lower box body (1), and heat dissipation holes are formed in one surface or multiple surfaces of the bottom of the lower box body (1) and used for dissipating heat of a heat dissipation fan (106).

6. The universal sample-adding low-temperature box for common molecular biology experiments according to any one of claims 1 to 5, wherein: the bottom of the lower box body (1) is provided with a rubber layer.

7. The universal sample-adding low-temperature box for common molecular biology experiments as claimed in claim 5, wherein: the refrigerator (10) comprises a cooling fan (106), a cooling fin (105), a semiconductor refrigerating sheet (103) and a cold guide sheet (102), wherein the semiconductor refrigerating sheet (103) is electrically connected with a power supply, the cold radiating surface of the semiconductor refrigerating sheet (103) is attached to one surface of the cold guide sheet (102), and the other surface of the cold guide sheet (102) is facing to the internal refrigerating space of the unit sample adding module (32) for refrigeration; the heat dissipation surface of the semiconductor refrigeration piece (103) is attached to one surface of the heat dissipation piece (105), the other surface of the heat dissipation piece (105) is attached to the air inlet surface of the heat dissipation fan (106), and the heat dissipation fan (106) is electrically connected with a power supply.

8. The universal sample-adding low-temperature box for common molecular biology experiments as claimed in claim 7, wherein: the periphery of cold conduction piece (102) be provided with and lead cold section of thick bamboo (104), the bottom of unit application of sample module (32) be provided with advance cold mouthful (31) with inside refrigeration space intercommunication, advance cold mouthful (31) and lead cold section of thick bamboo (104) and peg graft, the top of leading cold section of thick bamboo (104) be provided with collar (101), collar (101) on be provided with magnet, magnet adsorbs whole refrigerator (10) in the bottom of unit application of sample module (32), the bottom of unit application of sample module (32) is provided with the adsorbable metal area of magnet.

9. The universal sample-adding low-temperature box for common molecular biology experiments according to claim 8, wherein: unit application of sample module (32) including unit refrigeration case (34), unit refrigeration case (34) divide into the centrifuging tube and deposit the district with the cell culture board, the top of unit refrigeration case (34) in centrifuging tube storage area is roof (37), the inside of unit refrigeration case (34) is provided with deflector (35) and bottom plate (36), roof (37), deflector (35) and bottom plate (36) are from last downward interval arrangement in proper order, all run through on this roof (37) and deflector (35) and have vertical unanimous centrifuging tube to place the hole, the height of deflector (35) and bottom plate (36) in different centrifuging tube storage areas is inconsistent, the top of unit refrigeration case (34) in cell culture board storage area is roof (37), this roof (37) are dull and stereotyped.